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fractionation</title> <meta name="description" content="Search results for: NGL fractionation"> <meta name="keywords" content="NGL fractionation"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="NGL fractionation"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 93</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: NGL fractionation</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">33</span> Synergistic Effect of Curcumin and Insulin on GLUT4 Translocation in C2C12 Cell</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Javad%20Mohiti-Ardekani">Javad Mohiti-Ardekani</a>, <a href="https://publications.waset.org/abstracts/search?q=Shabodin%20Asadii"> Shabodin Asadii</a>, <a href="https://publications.waset.org/abstracts/search?q=Ali%20Moradi"> Ali Moradi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Curcumin, the yellow pigment in turmeric, has been shown as an anti-diabetic agent for centuries but only in recent few years, its mechanism of action has been under investigation. Some studies showed that curcumin might exert its anti-diabetic effect via increasing glucose transporter isotype-4 (GLUT4) gene and glycoprotein contents in cells. To investigate this possibility, we investigate the effects of extract and commercial curcumin with and without insulin on GLUT4 translocation from intracellular compartments of nuclear or endoplasmic reticulum membranes (N/ER) into the cytoplasmic membrane (CM). Methods and Material: C2C12 myoblastic cell line were seeded in DMEM plus 20 % FBS and differentiated to myotubes using 2 % horse serum. After myotubes formation, 40 µmolar Extract and Commercial curcumin, with or without insulin as intervention, and as control 1 % DMSO were added for 3 h. Cells were washed and homogenized followed by ultracentrifuge fractionation, protein separation by SDS-PAGE and GLUT4 detection using semi-quantitative Western blotting. Data analysis was done by two independent samples t-test for comparison of mean ± SD of GLUT4 percent in categories. GLUT4 contents were higher in CM groups curcumin and curcumin with insulin in comparison to 1 % DMSO-treated myotubes control group. Results: As our results have shown extract and commercial curcumin induces GLUT4 translocation from intra-cell into cell surface. The results have also shown synergic effect of curcumin on translocation of GLUT4 from intra-cell into cell surface in the presence of 100 nm insulin. Discussion: We conclude that curcumin may be a choice of type-2 diabetes mellitus treatment because its extract and commercial enhances GLUT4 contents in CM where it facilitates glucose entrance into the cell. However, it is necessary to trace the signaling pathways which are activated by curcumin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Curcumin" title="Curcumin">Curcumin</a>, <a href="https://publications.waset.org/abstracts/search?q=insulin" title=" insulin"> insulin</a>, <a href="https://publications.waset.org/abstracts/search?q=Diabetes%20type-2" title=" Diabetes type-2"> Diabetes type-2</a>, <a href="https://publications.waset.org/abstracts/search?q=GLUT4" title=" GLUT4"> GLUT4</a> </p> <a href="https://publications.waset.org/abstracts/41841/synergistic-effect-of-curcumin-and-insulin-on-glut4-translocation-in-c2c12-cell" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41841.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">243</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">32</span> Waters Colloidal Phase Extraction and Preconcentration: Method Comparison</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Emmanuelle%20Maria">Emmanuelle Maria</a>, <a href="https://publications.waset.org/abstracts/search?q=Pierre%20Cran%C3%A7on"> Pierre Crançon</a>, <a href="https://publications.waset.org/abstracts/search?q=Ga%C3%ABtane%20Lespes"> Gaëtane Lespes</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Colloids are ubiquitous in the environment and are known to play a major role in enhancing the transport of trace elements, thus being an important vector for contaminants dispersion. Colloids study and characterization are necessary to improve our understanding of the fate of pollutants in the environment. However, in stream water and groundwater, colloids are often very poorly concentrated. It is therefore necessary to pre-concentrate colloids in order to get enough material for analysis, while preserving their initial structure. Many techniques are used to extract and/or pre-concentrate the colloidal phase from bulk aqueous phase, but yet there is neither reference method nor estimation of the impact of these different techniques on the colloids structure, as well as the bias introduced by the separation method. In the present work, we have tested and compared several methods of colloidal phase extraction/pre-concentration, and their impact on colloids properties, particularly their size distribution and their elementary composition. Ultrafiltration methods (frontal, tangential and centrifugal) have been considered since they are widely used for the extraction of colloids in natural waters. To compare these methods, a ‘synthetic groundwater’ was used as a reference. The size distribution (obtained by Field-Flow Fractionation (FFF)) and the chemical composition of the colloidal phase (obtained by Inductively Coupled Plasma Mass Spectrometry (ICPMS) and Total Organic Carbon analysis (TOC)) were chosen as comparison factors. In this way, it is possible to estimate the pre-concentration impact on the colloidal phase preservation. It appears that some of these methods preserve in a more efficient manner the colloidal phase composition while others are easier/faster to use. The choice of the extraction/pre-concentration method is therefore a compromise between efficiency (including speed and ease of use) and impact on the structural and chemical composition of the colloidal phase. In perspective, the use of these methods should enhance the consideration of colloidal phase in the transport of pollutants in environmental assessment studies and forensics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chemical%20composition" title="chemical composition">chemical composition</a>, <a href="https://publications.waset.org/abstracts/search?q=colloids" title=" colloids"> colloids</a>, <a href="https://publications.waset.org/abstracts/search?q=extraction" title=" extraction"> extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=preconcentration%20methods" title=" preconcentration methods"> preconcentration methods</a>, <a href="https://publications.waset.org/abstracts/search?q=size%20distribution" title=" size distribution"> size distribution</a> </p> <a href="https://publications.waset.org/abstracts/81490/waters-colloidal-phase-extraction-and-preconcentration-method-comparison" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/81490.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">215</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">31</span> Uranium Migration Process: A Multi-Technique Investigation Strategy for a Better Understanding of the Role of Colloids</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Emmanuelle%20Maria">Emmanuelle Maria</a>, <a href="https://publications.waset.org/abstracts/search?q=Pierre%20Cran%C3%A7on"> Pierre Crançon</a>, <a href="https://publications.waset.org/abstracts/search?q=Ga%C3%ABtane%20Lespes"> Gaëtane Lespes</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The knowledge of uranium migration processes within underground environments is a major issue in the environmental risk assessment associated with nuclear activities. This process is identified as strongly controlled by adsorption mechanisms, thus leading to strongly delayed migration paths. Colloidal ligands are likely to significantly increase the mobility of uranium in natural environments. The ability of colloids to mobilize and transport uranium depends on their origin, their nature, their structure, their stability and their reactivity with uranium. Thus, the colloidal mobilization and transport properties are often described as site-specific. In this work, the colloidal phases of two leachates obtained from two different horizons of the same podzolic soil were characterized with a speciation approach. For this purpose, a multi-technique strategy was used, based on Field-Flow Fractionation coupled to Ultraviolet, Multi-Angle Light Scattering and Inductively Coupled Plasma Mass Spectrometry (AF4-UV-MALS-ICPMS), Transmission Electron Microscopy (TEM), Electrospray Ionization Orbitrap Mass Spectrometry (ESI-Orbitrap), and Time-Resolved Laser Fluorescence Spectroscopy (TRLFS-EEM). Thus, elemental composition, size distribution, microscopic structure, colloidal stability and possible organic and/or inorganic content of colloids were determined, as well as their association with uranium. The leachates exhibit differences in their physical and chemical characteristics, mainly in the nature of organic matter constituents. The multi-technique investigation strategy used provides original data about colloidal phase structure and composition, offering a new vision of the way the uranium can be mobilized and transported in the considered soil. This information is a real significant contribution opening the way to our understanding and predicting of the colloidal transport. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=colloids" title="colloids">colloids</a>, <a href="https://publications.waset.org/abstracts/search?q=migration" title=" migration"> migration</a>, <a href="https://publications.waset.org/abstracts/search?q=multi-technique" title=" multi-technique"> multi-technique</a>, <a href="https://publications.waset.org/abstracts/search?q=speciation" title=" speciation"> speciation</a>, <a href="https://publications.waset.org/abstracts/search?q=transport" title=" transport"> transport</a>, <a href="https://publications.waset.org/abstracts/search?q=uranium" title=" uranium"> uranium</a> </p> <a href="https://publications.waset.org/abstracts/80579/uranium-migration-process-a-multi-technique-investigation-strategy-for-a-better-understanding-of-the-role-of-colloids" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80579.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">144</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">30</span> Petrogenesis of the Neoproterozoic Rocks of Megele Area, Asosa, Western Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Temesgen%20Oljira">Temesgen Oljira</a>, <a href="https://publications.waset.org/abstracts/search?q=Olugbenga%20Akindeji%20Okunlola"> Olugbenga Akindeji Okunlola</a>, <a href="https://publications.waset.org/abstracts/search?q=Akinade%20Shadrach%20Olatunji"> Akinade Shadrach Olatunji</a>, <a href="https://publications.waset.org/abstracts/search?q=Dereje%20Ayalew"> Dereje Ayalew</a>, <a href="https://publications.waset.org/abstracts/search?q=Bekele%20Ayele%20Bedada"> Bekele Ayele Bedada</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Western Ethiopian Shield (WES) is underlain by volcano-sedimentary terranes, gneissic terranes, and ophiolitic rocks intruded by different granitoid bodies. For the past few years, Neoproterozoic rocks of the Megele area in the western part of the WES have been explored. Understanding the geology of the area and assessing the mineralized area's economic potential requires petrological, geochemical, and geological characterization of the Neoproterozoic granitoids and associated metavolcanic rocks. Thus, the geological, geochemical, and petrogenetic features of Neoproterozoic granitoids and associated metavolcanic rocks were elucidated using a combination of field mapping, petrological, and geochemical study. The Megele area is part of a low-grade volcano-sedimentary zone that has been intruded by mafic (dolerite dyke) and granitoid intrusions (granodiorite, diorite, granite gneiss). The granodiorite, associated diorite, and granite gneiss are calc-alkaline, peraluminous to slightly metaluminous, S-type granitoids formed in volcanic arc subduction (VAG) to syn-collisional (syn-COLD) tectonic setting by fractionation of LREE-enriched, HREE-depleted basaltic magma with considerable crustal input. While the metabasalt is sub-alkaline (tholeiitic), metaluminous bodies are generated at the mid-oceanic ridge tectonic setting by partially melting HREE-depleted and LREE-enriched basaltic magma. The reworking of sediment-loaded crustal blocks at depth in a subduction zone resulted in the production of S-type granitoids. This basaltic magma was supplied from an LREE-enriched, HREE-depleted mantle. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fractional%20crystallization" title="fractional crystallization">fractional crystallization</a>, <a href="https://publications.waset.org/abstracts/search?q=geochemistry" title=" geochemistry"> geochemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=Megele" title=" Megele"> Megele</a>, <a href="https://publications.waset.org/abstracts/search?q=petrogenesis" title=" petrogenesis"> petrogenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=s-type%20granite" title=" s-type granite"> s-type granite</a> </p> <a href="https://publications.waset.org/abstracts/149624/petrogenesis-of-the-neoproterozoic-rocks-of-megele-area-asosa-western-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/149624.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">128</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">29</span> DNA Fragmentation and Apoptosis in Human Colorectal Cancer Cell Lines by Sesamum indicum Dried Seeds</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohd%20Farooq%20Naqshbandi">Mohd Farooq Naqshbandi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The four fractions of aqueous extract of Sesame Seeds (Sesamum indicum L.) were studied for invitro DNA fragmentation, cell migration, and cellular apoptosis on SW480 and HTC116 human colorectal cancer cell lines. The seeds of Sesamum indicum were extracted with six solvents, including Methanol, Ethanol, Aqueous, Chloroform, Acetonitrile, and Hexane. The aqueous extract (IC₅₀ value 154 µg/ml) was found to be the most active in terms of cytotoxicity with SW480 human colorectal cancer cell lines. Further fractionation of this aqueous extract on flash chromatography gave four fractions. These four fractions were studied for anticancer and DNA binding studies. Cell viability was assessed by colorimetric assay (MTT). IC₅₀ values for all these four fractions ranged from 137 to 548 µg/mL for the HTC116 cancer cell line and 141 to 402 µg/mL for the SW480 cancer cell line. The four fractions showed good anticancer and DNA binding properties. The DNA binding constants ranged from 10.4 ×10⁴ 5 to 28.7 ×10⁴, showing good interactions with DNA. The DNA binding interactions were due to intercalative and π-π electron forces. The results indicate that aqueous extract fractions of sesame showed inhibition of cell migration of SW480 and HTC116 human colorectal cancer cell lines and induced DNA fragmentation and apoptosis. This was demonstrated by calculating the low wound closure percentage in cells treated with these fractions as compared to the control (80%). Morphological features of nuclei of cells treated with fractions revealed chromatin compression, nuclear shrinkage, and apoptotic body formation, which indicate cell death by apoptosis. The flow cytometer of fraction-treated cells of SW480 and HTC116 human colorectal cancer cell lines revealed death due to apoptosis. The results of the study indicate that aqueous extract of sesame seeds may be used to treat colorectal cancer. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sesamum%20indicum" title="Sesamum indicum">Sesamum indicum</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20migration%20inhibition" title=" cell migration inhibition"> cell migration inhibition</a>, <a href="https://publications.waset.org/abstracts/search?q=apoptosis%20induction" title=" apoptosis induction"> apoptosis induction</a>, <a href="https://publications.waset.org/abstracts/search?q=anticancer%20activity" title=" anticancer activity"> anticancer activity</a>, <a href="https://publications.waset.org/abstracts/search?q=colorectal%20cancer" title=" colorectal cancer"> colorectal cancer</a> </p> <a href="https://publications.waset.org/abstracts/156154/dna-fragmentation-and-apoptosis-in-human-colorectal-cancer-cell-lines-by-sesamum-indicum-dried-seeds" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/156154.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">88</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28</span> Feasibility of Phenolic Acids Rich Fraction from Gynura procumbens as Potential Antihyperlipidemic Agent</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vikneswaran%20Murugaiyah">Vikneswaran Murugaiyah</a>, <a href="https://publications.waset.org/abstracts/search?q=Sultan%20Ayesh%20Mohammed%20Saghir"> Sultan Ayesh Mohammed Saghir</a>, <a href="https://publications.waset.org/abstracts/search?q=Kisantini%20Murugesu"> Kisantini Murugesu</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohd.%20Zaini%20Asmawi"> Mohd. Zaini Asmawi</a>, <a href="https://publications.waset.org/abstracts/search?q=Amirin%20Sadikun"> Amirin Sadikun</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gynura procumbens is a popular medicinal plant used as a folk medicine in Southeast Asia to treat kidney diseases, diabetes mellitus and hyperlipidemia. The present study aims to investigate the antihyperlipidemic potential of phenolic acids rich fraction (PARF) from G. procumbens in chemically-induced acute and high fat diet-induced chronic hyperlipidemic rats. Ethanolic extract of G. procumbens leaves exhibited significant reductions in total cholesterol (TC) and triglycerides (TG) levels (P < 0.01 and P < 0.001, respectively) of poloxamer 407-induced rats compared to hyperlipidemic control after 58 h of treatment. Upon bioactivity guided fractionation the antihyperlipidemic activity was found to be concentrated in the PARF, which significantly reduced the TC and TG levels (P < 0.001). HPLC analysis revealed that 3,5-dicaffeoylquinic acid; 4,5-dicaffeoylquinic acid and chlorogenic acid are the major compounds in the PARF. Likewise, chlorogenic acid (60 mg/kg) exhibited significant reductions in TC and TG levels of hyperlipidemic rats (P < 0.001). Both chlorogenic acid and PARF significantly reduced LDL, VLDL and atherogenic index (P<0.01), while PARF increased the HDL (P < 0.01) compared to hyperlipidemic control. Both were found to be not cytotoxic against normal and cancer cell lines. In addition, LD50 of orally administered PARF was more than 5,000 mg/kg. Further investigation in high fat diet-induced chronic hyperlipidemic rats revealed that chronic administration of PARF dose-dependently restored the increase in lipids parameters. In summary, the phenolic acids rich fraction of G. procumbens leaves showed promising antihyperlipidemic effect in both chemically- and diet-induced hyperlipidemic rats that warrants further elucidation on its mechanisms of action. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Antihyperlipidemic" title="Antihyperlipidemic">Antihyperlipidemic</a>, <a href="https://publications.waset.org/abstracts/search?q=Gynura%20procumbens" title=" Gynura procumbens"> Gynura procumbens</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolic%20acids" title=" phenolic acids"> phenolic acids</a>, <a href="https://publications.waset.org/abstracts/search?q=chlorogenic%20acid" title=" chlorogenic acid"> chlorogenic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=poloxamer-407" title=" poloxamer-407"> poloxamer-407</a>, <a href="https://publications.waset.org/abstracts/search?q=high%20fat%20diet" title=" high fat diet"> high fat diet</a> </p> <a href="https://publications.waset.org/abstracts/61349/feasibility-of-phenolic-acids-rich-fraction-from-gynura-procumbens-as-potential-antihyperlipidemic-agent" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61349.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">231</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27</span> Protein-Enrichment of Oilseed Meals by Triboelectrostatic Separation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Javier%20Perez-Vaquero">Javier Perez-Vaquero</a>, <a href="https://publications.waset.org/abstracts/search?q=Katryn%20Junker"> Katryn Junker</a>, <a href="https://publications.waset.org/abstracts/search?q=Volker%20Lammers"> Volker Lammers</a>, <a href="https://publications.waset.org/abstracts/search?q=Petra%20Foerst"> Petra Foerst</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There is increasing importance to accelerate the transition to sustainable food systems by including environmentally friendly technologies. Our work focuses on protein enrichment and fractionation of agricultural side streams by dry triboelectrostatic separation technology. Materials are fed in particulate form into a system dispersed in a highly turbulent gas stream, whereby the high collision rate of particles against surfaces and other particles greatly enhances the electrostatic charge build-up over the particle surface. A subsequent step takes the charged particles to a delimited zone in the system where there is a highly uniform, intense electric field applied. Because the charge polarity acquired by a particle is influenced by its chemical composition, morphology, and structure, the protein-rich and fiber-rich particles of the starting material get opposite charge polarities, thus following different paths as they move through the region where the electric field is present. The output is two material fractions, which differ in their respective protein content. One is a fiber-rich, low-protein fraction, while the other is a high-protein, low-fiber composition. Prior to testing, materials undergo a milling process, and some samples are stored under controlled humidity conditions. In this way, the influence of both particle size and humidity content was established. We used two oilseed meals: lupine and rapeseed. In addition to a lab-scale separator to perform the experiments, the triboelectric separation process could be successfully scaled up to a mid-scale belt separator, increasing the mass feed from g/sec to kg/hour. The triboelectrostatic separation technology opens a huge potential for the exploitation of so far underutilized alternative protein sources. Agricultural side-streams from cereal and oil production, which are generated in high volumes by the industries, can further be valorized by this process. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bench-scale%20processing" title="bench-scale processing">bench-scale processing</a>, <a href="https://publications.waset.org/abstracts/search?q=dry%20separation" title=" dry separation"> dry separation</a>, <a href="https://publications.waset.org/abstracts/search?q=protein-enrichment" title=" protein-enrichment"> protein-enrichment</a>, <a href="https://publications.waset.org/abstracts/search?q=triboelectrostatic%20separation" title=" triboelectrostatic separation"> triboelectrostatic separation</a> </p> <a href="https://publications.waset.org/abstracts/139141/protein-enrichment-of-oilseed-meals-by-triboelectrostatic-separation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139141.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">190</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">26</span> Identification of Bioactive Metabolites from Ficus carica and Their Neuroprotective Effects of Alzheimer's Disease</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hanan%20Khojah">Hanan Khojah</a>, <a href="https://publications.waset.org/abstracts/search?q=RuAngelie%20Edrada-Ebel"> RuAngelie Edrada-Ebel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Neurodegenerative disease including Alzheimer’s disease is a major cause of long-term disability. Oxidative stress is frequently implicated as one of the key contributing factors to neurodegenerative diseases. Protection against neuronal damage remains a great challenge for researchers. Ficus carica (commonly known as fig) is a species of great antioxidant nutritional value comprising a protective mechanism against innumerable health disorders related to oxidative stress as well as Alzheimer’s disease. The purpose of this work was to characterize the non-polar active metabolites in Ficus carica endocarp, mesocarp, and exocarp. Crude extracts were prepared using several extraction solvents, which included 1:1 water: ethylacetate, acetone and methanol. The dried extracts were then solvent partitioned between equivalent amounts of water and ethylacetate. Purification and fractionation were accomplished by high-throughput chromatography. The isolated metabolites were tested on their effect on human neuroblastoma cell line by cell viability test and cell cytotoxicity assay with acrolein. Molecular weights of the active metabolites were determined via LC–HRESIMS and GC-EIMS. Metabolomic profiling was performed to identify the active metabolites by using differential expression analysis software (Mzmine) and SIMCA for multivariate analysis. Structural elucidation and identification of the interested active metabolites were studied by 1-D and 2-D NMR. Significant differences in bioactivity against a concentration-dependent assay on acrolein radicals were observed between the three fruit parts. However, metabolites obtained from mesocarp and the endocarp demonstrated bioactivity to scavenge ROS radical. NMR profiling demonstrated that aliphatic compounds such as γ-sitosterol tend to induce neuronal bioactivity and exhibited bioactivity on the cell viability assay. γ-Sitosterol was found in higher concentrations in the mesocarp and was considered as one of the major phytosterol in Ficus carica. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alzheimer" title="alzheimer">alzheimer</a>, <a href="https://publications.waset.org/abstracts/search?q=Ficus%20carica" title=" Ficus carica"> Ficus carica</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B3-Sitosterol" title=" γ-Sitosterol"> γ-Sitosterol</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolomics" title=" metabolomics"> metabolomics</a> </p> <a href="https://publications.waset.org/abstracts/61797/identification-of-bioactive-metabolites-from-ficus-carica-and-their-neuroprotective-effects-of-alzheimers-disease" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61797.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">344</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">25</span> Chemotrophic Signal Exchange between the Host Plant Helianthemum sessiliflorum and Terfezia boudieri</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Ben-Shabat">S. Ben-Shabat</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20Turgeman"> T. Turgeman</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20Leubinski"> O. Leubinski</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Roth-Bejerano"> N. Roth-Bejerano</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Kagan-Zur"> V. Kagan-Zur</a>, <a href="https://publications.waset.org/abstracts/search?q=Y.%20Sitrit"> Y. Sitrit</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The ectomycorrhizal (ECM) desert truffle Terfezia boudieri produces edible fruit bodies and forms symbiosis with its host plant Helianthemum sessiliflorum (Cistaceae) in the Negev desert of Israel. The symbiosis is vital for both partners' survival under desert conditions. Under desert habitat conditions, ECMs must form symbiosis before entering the dry season. To secure a successful encounter, in the course of evolution, both partners have responded by evolving special signals exchange that facilitates recognition. Members of the Cistaceae family serve as host plants for many important truffles. Conceivably, during evolution a common molecule present in Cistaceae plants was recruited to facilitate successful encounter with ectomycorrhizas. Arbuscular vesicular fungi (AM) are promiscuous in host preferences, in contrast, ECM fungi show specificity to host plants. Accordingly, we hypothesize that H. sessiliflorum secretes a chemotrophic-signaling, which is common to plants hosting ECM fungi belonging to the Pezizales. However, thus far no signaling molecules have been identified in ECM fungi. We developed a bioassay for chemotrophic activity. Fractionation of root exudates revealed a substance with chemotrophic activity and molecular mass of 534. Following the above concept, screening the transcriptome of Terfezia, grown under chemoattraction, discovered genes showing high homology to G proteins-coupled receptors of plant pathogens involved in positive chemotaxis and chemotaxis suppression. This study aimed to identify the active molecule using analytical methods (LC-MS, NMR etc.). This should contribute to our understanding of how ECM fungi communicate with their hosts in the rhizosphere. In line with the ability of Terfezia to form also endomycorrhizal symbiosis like AM fungi, analysis of the mechanisms may likewise be applicable to AM fungi. Developing methods to manipulate fungal growth by the chemoattractant can open new ways to improve inoculation of plants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chemotrophic%20signal" title="chemotrophic signal">chemotrophic signal</a>, <a href="https://publications.waset.org/abstracts/search?q=Helianthemum%20sessiliflorum" title=" Helianthemum sessiliflorum"> Helianthemum sessiliflorum</a>, <a href="https://publications.waset.org/abstracts/search?q=Terfezia%20boudieri" title=" Terfezia boudieri"> Terfezia boudieri</a>, <a href="https://publications.waset.org/abstracts/search?q=ECM" title=" ECM"> ECM</a> </p> <a href="https://publications.waset.org/abstracts/15364/chemotrophic-signal-exchange-between-the-host-plant-helianthemum-sessiliflorum-and-terfezia-boudieri" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15364.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">409</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">24</span> Technico-Economical Study of a Rapeseed Based Biorefinery Using High Voltage Electrical Discharges and Ultrasounds as Pretreatment Technologies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marwa%20Brahim">Marwa Brahim</a>, <a href="https://publications.waset.org/abstracts/search?q=Nicolas%20Brosse"> Nicolas Brosse</a>, <a href="https://publications.waset.org/abstracts/search?q=Nadia%20Boussetta"> Nadia Boussetta</a>, <a href="https://publications.waset.org/abstracts/search?q=Nabil%20Grimi"> Nabil Grimi</a>, <a href="https://publications.waset.org/abstracts/search?q=Eugene%20Vorobiev"> Eugene Vorobiev</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rapeseed plant is an established product in France which is mainly dedicated to oil production. However, the economic potential of residues from this industry (rapeseed hulls, rapeseed cake, rapeseed straw etc.), has not been fully exploited. Currently, only low-grade applications are found in the market. As a consequence, it was deemed of interest to develop a technological platform aiming to convert rapeseed residues into value- added products. Specifically, a focus is given on the conversion of rapeseed straw into valuable molecules (e.g. lignin, glucose). Existing pretreatment technologies have many drawbacks mainly the production of sugar degradation products that limit the effectiveness of saccharification and fermentation steps in the overall scheme of the lignocellulosic biorefinery. In addition, the viability of fractionation strategies is a challenge in an environmental context increasingly standardized. Hence, the need to find cleaner alternatives with comparable efficiency by implementing physical phenomena that could destabilize the structural integrity of biomass without necessarily using chemical solvents. To meet environmental standards increasingly stringent, the present work aims to study the new pretreatment strategies involving lower consumption of chemicals with an attenuation of the severity of the treatment. These strategies consist on coupling physical treatments either high voltage electrical discharges or ultrasounds to conventional chemical pretreatments (soda and organosolv). Ultrasounds treatment is based on the cavitation phenomenon, and high voltage electrical discharges cause an electrical breakdown accompanied by many secondary phenomena. The choice of process was based on a technological feasibility study taking into account the economic profitability of the whole chain after products valorization. Priority was given to sugars valorization into bioethanol and lignin sale. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=high%20voltage%20electrical%20discharges" title="high voltage electrical discharges">high voltage electrical discharges</a>, <a href="https://publications.waset.org/abstracts/search?q=organosolv" title=" organosolv"> organosolv</a>, <a href="https://publications.waset.org/abstracts/search?q=pretreatment%20strategies" title=" pretreatment strategies"> pretreatment strategies</a>, <a href="https://publications.waset.org/abstracts/search?q=rapeseed%20straw" title=" rapeseed straw"> rapeseed straw</a>, <a href="https://publications.waset.org/abstracts/search?q=soda" title=" soda"> soda</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasounds" title=" ultrasounds"> ultrasounds</a> </p> <a href="https://publications.waset.org/abstracts/58493/technico-economical-study-of-a-rapeseed-based-biorefinery-using-high-voltage-electrical-discharges-and-ultrasounds-as-pretreatment-technologies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58493.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">362</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">23</span> Oxygen and Sulfur Isotope Composition of Gold Bearing Granite Gneiss and Quartz Veins of Megele Area, Western Ethiopia: Implication for Fluid Source</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Temesgen%20Oljira">Temesgen Oljira</a>, <a href="https://publications.waset.org/abstracts/search?q=Olugbenga%20Akindeji%20Okunlola"> Olugbenga Akindeji Okunlola</a>, <a href="https://publications.waset.org/abstracts/search?q=Akinade%20Shadrach%20Olatunji"> Akinade Shadrach Olatunji</a>, <a href="https://publications.waset.org/abstracts/search?q=Dereje%20Ayalew"> Dereje Ayalew</a>, <a href="https://publications.waset.org/abstracts/search?q=Bekele%20A.%20Bedada"> Bekele A. Bedada</a>, <a href="https://publications.waset.org/abstracts/search?q=Tasin%20Godlove%20Bafon"> Tasin Godlove Bafon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Megele area gold-bearing Neoproterozoic rocks in the Western Ethiopian Shield has been under exploration for the last few decades. The geochemical and ore petrological characterization of the gold-bearing granite gneiss and associated quartz vein is crucial in understanding the gold's genesis. The present study concerns the ore petrological, geochemical, and stable O2 and S characterization of the gold-bearing granite gneiss and associated quartz vein. This area is known for its long history of placer gold mining. The presence of quartz veins of different generations and orientations, visible sulfide mineralization, and oxidation suggests that the Megele area is geologically fertile for mineralization. The Au and base metals analysis also indicate that Megele area rocks are characterized by Cu (2-22 ppm av. 7.83 ppm), Zn (2-53 ppm av. 29.33 ppm), Co (1-27 ppm av. 13.33 ppm), Ni (2-16 ppm av. 10 ppm), Pb (5-10 ppm av. 8.33 ppm), Au (1-5 ppb av. 2.11 ppb), Ag (0.5 ppm), As (5-12 ppm av. 7.83 ppm), Cd (0.5ppm), Li (0.5 ppm), Mo (1-4 ppm av. 1.6 ppm), Sc (5-13 ppm av. 9.3 ppm), and Tl (10 ppm). The oxygen isotope (δ18O) values of gold-bearing granite gneiss and associated quartz veins range from +8.6 to +11.5 ‰, suggesting the mixing of metamorphic water with magmatic water within the ore-forming fluid. The Sulfur isotope (δ34S) values of gold-bearing granite gneiss range from -1.92 to -0.45 ‰ (mean value of -1.13 ‰) indicating the narrow range of value. This suggests that the sulfides have been precipitated from the fluid system originating from a single source of the magmatic component under sulfur isotopic fractionation equilibrium condition. The tectonic setting of the host rocks, the occurrence of ore bodies, mineral assemblages of the host rocks and proposed ore-forming fluids of the Megele area gold prospects have similarities with features of orogenic gold deposit. The δ18O and δ34S isotopic values also suggested a metamorphic origin with the magmatic components. Thus, the Megele gold prospect could be related to an orogenic gold deposit related to metamorphism and associated intrusions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fluid%20source" title="fluid source">fluid source</a>, <a href="https://publications.waset.org/abstracts/search?q=gold%20mineralization" title=" gold mineralization"> gold mineralization</a>, <a href="https://publications.waset.org/abstracts/search?q=oxygen%20isotope" title=" oxygen isotope"> oxygen isotope</a>, <a href="https://publications.waset.org/abstracts/search?q=stable%20isotope" title=" stable isotope"> stable isotope</a>, <a href="https://publications.waset.org/abstracts/search?q=sulfur%20isotope" title=" sulfur isotope"> sulfur isotope</a> </p> <a href="https://publications.waset.org/abstracts/169023/oxygen-and-sulfur-isotope-composition-of-gold-bearing-granite-gneiss-and-quartz-veins-of-megele-area-western-ethiopia-implication-for-fluid-source" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/169023.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">73</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">22</span> Canthin-6-One Alkaloid Inhibits NF-κB and AP-1 Activity: An Inhibitory Action At Transcriptional Level</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fadia%20Gafri">Fadia Gafri</a>, <a href="https://publications.waset.org/abstracts/search?q=Kathryn%20Mckintosh"> Kathryn Mckintosh</a>, <a href="https://publications.waset.org/abstracts/search?q=Louise%20Young"> Louise Young</a>, <a href="https://publications.waset.org/abstracts/search?q=Alan%20Harvey"> Alan Harvey</a>, <a href="https://publications.waset.org/abstracts/search?q=Simon%20Mackay"> Simon Mackay</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrew%20Paul"> Andrew Paul</a>, <a href="https://publications.waset.org/abstracts/search?q=Robin%20Plevin"> Robin Plevin </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Nuclear factor-kappa B (NF-κB) is a ubiquitous transcription factor found originally to play a key role in regulating inflammation. However considerable evidence links this pathway to the suppression of apoptosis, cellular transformation, proliferation and invasion (Aggarwal et al., 2006). Moreover, recent studies have also linked inflammation to cancer progression making NF-κB overall a promising therapeutic target for drug discovery (Dobrovolskaia & Kozlov, 2005). In this study we examined the effect of the natural product canthin-6-one (SU182) as part of a CRUK small molecule drug discovery programme for effects upon the NF-κB pathway. Initial studies demonstrated that SU182 was found to have good potency against the inhibitory kappa B kinases (IKKs) at 30M in vitro. However, at concentrations up to 30M, SU182 had no effect upon TNFα stimulated loss in cellular IκBα or p65 phosphorylation in the keratinocyte cell line NCTC2544. Nevertheless, 30M SU182 reduced TNF-α / PMA-induced NF-κB-linked luciferase reporter activity to (22.9 ± 5%) and (34.6± 3 %, P<0.001) respectively, suggesting an action downstream of IKK signalling. Indeed, SU182 neither decreased NF-κB-DNA binding as assayed by EMSA nor prevented the translocation of p65 (NF-κB) to the nucleus assessed by immunofluorescence and subcellular fractionation. In addition to the inhibition of transcriptional activity of TNFα-induced NF-κB reporter activity SU182 significantly reduced PMA-induced AP-1-linked luciferase reporter activity to about (48± 9% at 30M, P<0.001) . This mode of inhibition was not sufficient to prevent the activation of NF-κB dependent induction of other proteins such as COX-2 and iNOS, or activated MAP kinases (p38, JNK and ERK1/2) in LPS stimulated RAW 264.7 macrophages. Taken together these data indicate the potential for SU182 to interfere with the transcription factors NF-κB and AP-1 at transcriptional level. However, no potential anti-inflammatory effect was indicated, further investigation for other NF-κB dependent proteins linked to survival are also required to identify the exact mechanism of action. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Canthin-6-one" title="Canthin-6-one">Canthin-6-one</a>, <a href="https://publications.waset.org/abstracts/search?q=NF-%CE%BAB" title=" NF-κB"> NF-κB</a>, <a href="https://publications.waset.org/abstracts/search?q=AP-1" title=" AP-1"> AP-1</a>, <a href="https://publications.waset.org/abstracts/search?q=phosphorylation" title=" phosphorylation"> phosphorylation</a>, <a href="https://publications.waset.org/abstracts/search?q=Nuclear%20translocation" title=" Nuclear translocation"> Nuclear translocation</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA-binding%20activity" title=" DNA-binding activity"> DNA-binding activity</a>, <a href="https://publications.waset.org/abstracts/search?q=inflammatory%20proteins." title=" inflammatory proteins."> inflammatory proteins.</a> </p> <a href="https://publications.waset.org/abstracts/20446/canthin-6-one-alkaloid-inhibits-nf-kb-and-ap-1-activity-an-inhibitory-action-at-transcriptional-level" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20446.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">458</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">21</span> Septin 11, Cytoskeletal Protein Involved in the Regulation of Lipid Metabolism in Adipocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Moreno-Castellanos">Natalia Moreno-Castellanos</a>, <a href="https://publications.waset.org/abstracts/search?q=Amaia%20Rodriguez"> Amaia Rodriguez</a>, <a href="https://publications.waset.org/abstracts/search?q=Gema%20Fr%C3%BChbeck"> Gema Frühbeck</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: In adipocytes, the cytoskeleton undergoes important expression and distribution in adipocytes rearrangements during adipogenesis and in obesity. Indeed, a role for these proteins in the regulation of adipocyte differentiation and response to insulin has been demonstrated. Recently, septins have been considered as new components of the cytoskeletal network that interact with other cytoskeletal elements (actin and tubulin) profoundly modifying their dynamics. However, these proteins have not been characterized as yet in adipose tissue. In this work, were examined the cellular, molecular and functional features of a member of this family, septin 11 (SEPT11), in adipocytes and evaluated the impact of obesity on the expression of this protein in human adipose tissue. Methods: Adipose gene and protein expression levels of SEPT11 were analysed in human samples. SEPT11 distribution was evaluated by immunocytochemistry, electronic microscopy, and subcellular fractionation techniques. GST-pull down, immunoprecipitation and a Yeast-Two Hybrid (Y2H) screening were used to identify the SEPT11 interactome. Gene silencing was employed to assess the role of SEPT11 in the regulation of insulin signaling and lipid metabolism in adipocytes. Results: SEPT11 is expressed in human adipocytes, and its levels increased in both omental and subcutaneous adipose tissue in obesity, with SEPT11 mRNA content positively correlating with parameters of insulin resistance in subcutaneous fat. In non-stimulated adipocytes, SEPT11 immunoreactivity showed a ring-like distribution at the cell surface and associated to caveolae. Biochemical analyses showed that SEPT11 interacted with the main component of caveolae, caveolin-1 (CAV1) as well as with the fatty acid-binding protein, FABP5. Notably, the three proteins redistributed and co-localized at the surface of lipid droplets upon exposure of adipocytes to oleate. In this line, SEPT11 silencing in 3T3-L1 adipocytes impaired insulin signaling and decreased insulin-induced lipogenesis. Conclusions: Those findings demonstrate that SEPT11 is a novel component of the adipocyte cytoskeleton that plays an important role in the regulation of lipid traffic, metabolism and can thus represent a potential biomarker of insulin resistance in obesity in adipocytes through its interaction with both CAV1 and FABP5. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=caveolae" title="caveolae">caveolae</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid%20metabolism" title=" lipid metabolism"> lipid metabolism</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=septins" title=" septins"> septins</a> </p> <a href="https://publications.waset.org/abstracts/79045/septin-11-cytoskeletal-protein-involved-in-the-regulation-of-lipid-metabolism-in-adipocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/79045.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">212</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">20</span> Economic Evaluation of an Advanced Bioethanol Manufacturing Technology Using Maize as a Feedstock in South Africa</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ayanda%20Ndokwana">Ayanda Ndokwana</a>, <a href="https://publications.waset.org/abstracts/search?q=Stanley%20Fore"> Stanley Fore</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Industrial prosperity and rapid expansion of human population in South Africa over the past two decades, have increased the use of conventional fossil fuels such as crude oil, coal and natural gas to meet the country’s energy demands. However, the inevitable depletion of fossil fuel reserves, global volatile oil price and large carbon footprint are some of the crucial reasons the South African Government needs to make a considerable investment in the development of the biofuel industry. In South Africa, this industry is still at the introductory stage with no large scale manufacturing plant that has been commissioned yet. Bioethanol is a potential replacement of gasoline which is a fossil fuel that is used in motor vehicles. Using bioethanol for the transport sector as a source of fuel will help Government to save heavy foreign exchange incurred during importation of oil and create many job opportunities in rural farming. In 2007, the South African Government developed the National Biofuels Industrial Strategy in an effort to make provision for support and attract investment in bioethanol production. However, capital investment in the production of bioethanol on a large scale, depends on the sound economic assessment of the available manufacturing technologies. The aim of this study is to evaluate the profitability of an advanced bioethanol manufacturing technology which uses maize as a feedstock in South Africa. The impact of fiber or bran fractionation in this technology causes it to possess a number of merits such as energy efficiency, low capital expenditure, and profitability compared to a conventional dry-mill bioethanol technology. Quantitative techniques will be used to collect and analyze numerical data from suitable organisations in South Africa. The dependence of three profitability indicators such as the Discounted Payback Period (DPP), Net Present Value (NPV) and Return On Investment (ROI) on plant capacity will be evaluated. Profitability analysis will be done on the following plant capacities: 100 000 ton/year, 150 000 ton/year and 200 000 ton/year. The plant capacity with the shortest Discounted Payback Period, positive Net Present Value and highest Return On Investment implies that a further consideration in terms of capital investment is warranted. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioethanol" title="bioethanol">bioethanol</a>, <a href="https://publications.waset.org/abstracts/search?q=economic%20evaluation" title=" economic evaluation"> economic evaluation</a>, <a href="https://publications.waset.org/abstracts/search?q=maize" title=" maize"> maize</a>, <a href="https://publications.waset.org/abstracts/search?q=profitability%20indicators" title=" profitability indicators"> profitability indicators</a> </p> <a href="https://publications.waset.org/abstracts/54688/economic-evaluation-of-an-advanced-bioethanol-manufacturing-technology-using-maize-as-a-feedstock-in-south-africa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54688.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">233</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">19</span> Single and Sequential Extraction for Potassium Fractionation and Nano-Clay Flocculation Structure</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chakkrit%20Poonpakdee">Chakkrit Poonpakdee</a>, <a href="https://publications.waset.org/abstracts/search?q=Jing-Hua%20Tzen"> Jing-Hua Tzen</a>, <a href="https://publications.waset.org/abstracts/search?q=Ya-Zhen%20Huang"> Ya-Zhen Huang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yao-Tung%20Lin"> Yao-Tung Lin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Potassium (K) is a known macro nutrient and essential element for plant growth. Single leaching and modified sequential extraction schemes have been developed to estimate the relative phase associations of soil samples. The sequential extraction process is a step in analyzing the partitioning of metals affected by environmental conditions, but it is not a tool for estimation of K bioavailability. While, traditional single leaching method has been used to classify K speciation for a long time, it depend on its availability to the plants and use for potash fertilizer recommendation rate. Clay mineral in soil is a factor for controlling soil fertility. The change of the micro-structure of clay minerals during various environment (i.e. swelling or shrinking) is characterized using Transmission X-Ray Microscopy (TXM). The objective of this study are to 1) compare the distribution of K speciation between single leaching and sequential extraction process 2) determined clay particle flocculation structure before/after suspension with K+ using TXM. Four tropical soil samples: farming without K fertilizer (10 years), long term applied K fertilizer (10 years; 168-240 kg K2O ha-1 year-1), red soil (450-500 kg K2O ha-1 year-1) and forest soil were selected. The results showed that the amount of K speciation by single leaching method were high in mineral K, HNO3 K, Non-exchangeable K, NH4OAc K, exchangeable K and water soluble K respectively. Sequential extraction process indicated that most K speciations in soil were associated with residual, organic matter, Fe or Mn oxide and exchangeable fractions and K associate fraction with carbonate was not detected in tropical soil samples. In farming long term applied K fertilizer and red soil were higher exchangeable K than farming long term without K fertilizer and forest soil. The results indicated that one way to increase the available K (water soluble K and exchangeable K) should apply K fertilizer and organic fertilizer for providing available K. The two-dimension of TXM image of clay particles suspension with K+ shows that the aggregation structure of clay mineral closed-void cellular networks. The porous cellular structure of soil aggregates in 1 M KCl solution had large and very larger empty voids than in 0.025 M KCl and deionized water respectively. TXM nanotomography is a new technique can be useful in the field as a tool for better understanding of clay mineral micro-structure. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=potassium" title="potassium">potassium</a>, <a href="https://publications.waset.org/abstracts/search?q=sequential%20extraction%20process" title=" sequential extraction process"> sequential extraction process</a>, <a href="https://publications.waset.org/abstracts/search?q=clay%20mineral" title=" clay mineral"> clay mineral</a>, <a href="https://publications.waset.org/abstracts/search?q=TXM" title=" TXM"> TXM</a> </p> <a href="https://publications.waset.org/abstracts/43853/single-and-sequential-extraction-for-potassium-fractionation-and-nano-clay-flocculation-structure" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43853.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">289</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">18</span> Bioactivities and Phytochemical Studies of Acrocarpus fraxinifolius Bark Wight and Arn</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=H.%20M.%20El-Rafie">H. M. El-Rafie</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20H.%20Abou%20Zeid"> A. H. Abou Zeid</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20S.%20Mohammed"> R. S. Mohammed</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20A.%20Sleem"> A. A. Sleem</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Acrocarpus is a genus of flowering plants in the legume family Fabaceae which considered as a large and economically important family. This study aimed to investigate the phytoconstituents of the petroleum ether extract (PEE) of Acrocarpus fraxinofolius bark by Gas chromatography coupled with mass spectrometry (GC/MS) analysis of its fractions (fatty acid and unsaponifiable matter). Concerning this, identification of 52 compounds constituting 97.03 % of the total composition of the unsaponifiable matter fraction. Cycloeucalenol was found to be the major compound representing 32.52% followed by 4a, 14a-dimethyl-A8~24(28)-ergostadien (26.50%) and ß-sitosterol(13.74%), furthermore Gas liquid chromatography (GLC) analysis of the sterol fraction revealed the identification of cholesterol (7.22 %), campesterol (13.30 %), stigmasterol (10.00 %) and β - sitosterol (69.48 %). Meanwhile, the identification of 33 fatty acids representing 90.71% of the total fatty acid constituents. Methyl-9,12-octadecadienoate (40.39%) followed by methyl hexadecanoate (23.64%) were found to be the major compounds. On the other hand, column chromatography and Thin layer chromatography (TLC) fractionation of PEE separate the triterpenoid: 21β-hydroxylup-20(29)-en-3-one and β- amyrin which were structurally identified by spectroscopic analysis (NMR, MS and IR). PEE has been biologically evaluated for 1: management of diabetes in alloxan induced diabetic rats 2: cytotoxic activity against four human tumor cell lines (Cervix carcinoma cell line[HELA], Breast carcinoma cell line [MCF7], Liver carcinoma cell line[HEPG2] and Colon carcinoma cell line[HCT-116] 3: hepatoprotective activity against CCl4-induced hepatotoxicity in rats and the activity was studied by assaying the serum marker enzymes like AST, ALT, and ALP. Concerning this, the anti-diabetic activity exhibited by 100mg of PEE extract was 74.38% relative to metformin (100% potency). It also showed a significant anti-proliferative activity against MCF-7 (IC50= 2.35µg), Hela(IC50=3.85µg) and HEPG-2 (IC50= 9.54µg) compared with Doxorubicin as reference drug. The hepatoprotective activity was evidenced by significant decrease in liver function enzymes, i.e. AST, ALT and ALP by (29.18%, 28.26%, and 34.11%, respectively using silymarin as the reference drug, compared to their concentration levels in an untreated group with liver damage induced by CCl₄. This study was performed for the first time on the bark of this species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acrocarpus%20fraxinofolius" title="Acrocarpus fraxinofolius">Acrocarpus fraxinofolius</a>, <a href="https://publications.waset.org/abstracts/search?q=antidiabetic" title=" antidiabetic"> antidiabetic</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxic" title=" cytotoxic"> cytotoxic</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatoprotective" title=" hepatoprotective"> hepatoprotective</a> </p> <a href="https://publications.waset.org/abstracts/72471/bioactivities-and-phytochemical-studies-of-acrocarpus-fraxinifolius-bark-wight-and-arn" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72471.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">196</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">17</span> Colloids and Heavy Metals in Groundwaters: Tangential Flow Filtration Method for Study of Metal Distribution on Different Sizes of Colloids</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jiancheng%20Zheng">Jiancheng Zheng</a> </p> <p class="card-text"><strong>Abstract:</strong></p> When metals are released into water from mining activities, they undergo changes chemically, physically and biologically and then may become more mobile and transportable along the waterway from their original sites. Natural colloids, including both organic and inorganic entities, are naturally occurring in any aquatic environment with sizes in the nanometer range. Natural colloids in a water system play an important role, quite often a key role, in binding and transporting compounds. When assessing and evaluating metals in natural waters, their sources, mobility, fate, and distribution patterns in the system are the major concerns from the point of view of assessing environmental contamination and pollution during resource development. There are a few ways to quantify colloids and accordingly study how metals distribute on different sizes of colloids. Current research results show that the presence of colloids can enhance the transport of some heavy metals in water, while heavy metals may also have an influence on the transport of colloids when cations in the water system change colloids and/or the ion strength of the water system changes. Therefore, studies into the relationship between different sizes of colloids and different metals in a water system are necessary and needed as natural colloids in water systems are complex mixtures of both organic and inorganic as well as biological materials. Their stability could be sensitive to changes in their shapes, phases, hardness and functionalities due to coagulation and deposition et al. and chemical, physical, and biological reactions. Because metal contaminants’ adsorption on surfaces of colloids is closely related to colloid properties, it is desired to fraction water samples as soon as possible after a sample is taken in the natural environment in order to avoid changes to water samples during transportation and storage. For this reason, this study carried out groundwater sample processing in the field, using Prep/Scale tangential flow filtration systems with 3-level cartridges (1 kDa, 10 kDa and 100 kDa). Groundwater samples from seven sites at Fort MacMurray, Alberta, Canada, were fractionated during the 2015 field sampling season. All samples were processed within 3 hours after samples were taken. Preliminary results show that although the distribution pattern of metals on colloids may vary with different samples taken from different sites, some elements often tend to larger colloids (such as Fe and Re), some to finer colloids (such as Sb and Zn), while some of them mainly in the dissolved form (such as Mo and Be). This information is useful to evaluate and project the fate and mobility of different metals in the groundwaters and possibly in environmental water systems. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=metal" title="metal">metal</a>, <a href="https://publications.waset.org/abstracts/search?q=colloid" title=" colloid"> colloid</a>, <a href="https://publications.waset.org/abstracts/search?q=groundwater" title=" groundwater"> groundwater</a>, <a href="https://publications.waset.org/abstracts/search?q=mobility" title=" mobility"> mobility</a>, <a href="https://publications.waset.org/abstracts/search?q=fractionation" title=" fractionation"> fractionation</a>, <a href="https://publications.waset.org/abstracts/search?q=sorption" title=" sorption"> sorption</a> </p> <a href="https://publications.waset.org/abstracts/168859/colloids-and-heavy-metals-in-groundwaters-tangential-flow-filtration-method-for-study-of-metal-distribution-on-different-sizes-of-colloids" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/168859.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">362</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">16</span> Purification of Bacillus Lipopeptides for Diverse Applications</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vivek%20Rangarajan">Vivek Rangarajan</a>, <a href="https://publications.waset.org/abstracts/search?q=Kim%20G.%20Clarke"> Kim G. Clarke</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bacillus lipopeptides are biosurfactants with wide ranging applications in the medical, food, agricultural, environmental and cosmetic industries. They are produced as a mix of three families, surfactin, iturin and fengycin, each comprising a large number of homologues of varying functionalities. Consequently, the method and degree of purification of the lipopeptide cocktail becomes particularly important if the functionality of the lipopeptide end-product is to be maximized for the specific application. However, downstream processing of Bacillus lipopeptides is particularly challenging due to the subtle variations observed in the different lipopeptide homologues and isoforms. To date, the most frequently used lipopeptide purification operations have been acid precipitation, solvent extraction, membrane ultrafiltration, adsorption and size exclusion. RP-HPLC (reverse phase high pressure liquid chromatography) also has potential for fractionation of the lipopeptide homologues. In the studies presented here, membrane ultrafiltration and RP-HPLC were evaluated for lipopeptide purification to different degrees of purities for maximum functionality. Batch membrane ultrafiltration using 50 kDa polyether sulphone (PES) membranes resulted in lipopeptide recovery of about 68% for surfactin and 82 % for fengycin. The recovery was further improved to 95% by using size-conditioned lipopeptide micelles. The conditioning of lipopeptides with Ca2+ ions resulted in uniformly sized micelles with average size of 96.4 nm and a polydispersity index of 0.18. The size conditioning also facilitated removal of impurities (molecular weight ranging between 2335-3500 Da) through operation of the system under dia-filtration mode, in a way similar to salt removal from protein by dialysis. The resultant purified lipopeptide was devoid of macromolecular impurities and could ideally suit applications in the cosmetic and food industries. Enhanced purification using RP-HPLC was carried out in an analytical C18 column, with the aim to fractionate lipopeptides into their constituent homologues. The column was eluted with mobile phase comprising acetonitrile and water over an acetonitrile gradient, 35% - 80%, over 70 minutes. The gradient elution program resulted in as many as 41 fractions of individual lipopeptide homologues. The efficacy test of these fractions against fungal phytopathogens showed that first 21 fractions, identified to be homologues of iturins and fengycins, displayed maximum antifungal activities, suitable for biocontrol in the agricultural industry. Thus, in the current study, the downstream processing of lipopeptides leading to tailor-made products for selective applications was demonstrated using two major downstream unit operations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacillus%20lipopeptides" title="bacillus lipopeptides">bacillus lipopeptides</a>, <a href="https://publications.waset.org/abstracts/search?q=membrane%20ultrafiltration" title=" membrane ultrafiltration"> membrane ultrafiltration</a>, <a href="https://publications.waset.org/abstracts/search?q=purification" title=" purification"> purification</a>, <a href="https://publications.waset.org/abstracts/search?q=RP-HPLC" title=" RP-HPLC"> RP-HPLC</a> </p> <a href="https://publications.waset.org/abstracts/59828/purification-of-bacillus-lipopeptides-for-diverse-applications" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59828.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">205</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">15</span> Oat βeta Glucan Attenuates the Development of Atherosclerosis and Improves the Intestinal Barrier Function by Reducing Bacterial Endotoxin Translocation in APOE-/- MICE</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dalal%20Alghawas">Dalal Alghawas</a>, <a href="https://publications.waset.org/abstracts/search?q=Jetty%20Lee"> Jetty Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Kaisa%20Poutanen"> Kaisa Poutanen</a>, <a href="https://publications.waset.org/abstracts/search?q=Hani%20El-Nezami"> Hani El-Nezami</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Oat β-glucan a water soluble non starch linear polysaccharide has been approved as a cholesterol lowering agent by various food safety administrations and is commonly used to reduce the risk of heart disease. The molecular weight of oat β-glucan can vary depending on the extraction and fractionation methods. It is not clear whether the molecular weight has a significant impact at reducing the acceleration of atherosclerosis. The aim of this study was to investigate three different oat β-glucan fractionations on the development of atherosclerosis in vivo. With special focus on plaque stability and the intestinal barrier function. To test this, ApoE-/- female mice were fed a high fat diet supplemented with oat bran, high molecular weight (HMW) oat β-glucan fractionate and low molecular weight (LMW) oat β-glucan fractionate for 16 weeks. Atherosclerosis risk markers were measured in the plasma, heart and aortic tree. Plaque size was measured in the aortic root and aortic tree. ICAM-1, VCAM-1, E-Selectin, P-Selectin, protein levels were assessed from the aortic tree to determine plaque stability at 16 weeks. The expression of p22phox at the aortic root was evaluated to study the NADPH oxidase complex involved in nitric oxide bioavailability and vascular elasticity. The tight junction proteins E-cadherin and beta-catenin from western blot analyses were analysed as an intestinal barrier function test. Plasma LPS, intestinal D-lactate levels and hepatic FMO gene expression were carried out to confirm whether the compromised intestinal barrier lead to endotoxemia. The oat bran and HMW oat β-glucan diet groups were more effective than the LMW β-glucan diet group at reducing the plaque size and showed marked improvements in plaque stability. The intestinal barrier was compromised for all the experimental groups however the endotoxemia levels were higher in the LMW β-glucan diet group. The oat bran and HMW oat β-glucan diet groups were more effective at attenuating the development of atherosclerosis. Reasons for this could be due to the LMW oat β-glucan diet group’s low viscosity in the gut and the inability to block the reabsorption of cholesterol. Furthermore the low viscosity may allow more bacterial endotoxin translocation through the impaired intestinal barrier. In future food technologists should carefully consider how to incorporate LMW oat β-glucan as a health promoting food. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Atherosclerosis" title="Atherosclerosis">Atherosclerosis</a>, <a href="https://publications.waset.org/abstracts/search?q=beta%20glucan" title=" beta glucan"> beta glucan</a>, <a href="https://publications.waset.org/abstracts/search?q=endotoxemia" title=" endotoxemia"> endotoxemia</a>, <a href="https://publications.waset.org/abstracts/search?q=intestinal%20barrier%20function" title=" intestinal barrier function"> intestinal barrier function</a> </p> <a href="https://publications.waset.org/abstracts/26279/oat-veta-glucan-attenuates-the-development-of-atherosclerosis-and-improves-the-intestinal-barrier-function-by-reducing-bacterial-endotoxin-translocation-in-apoe-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26279.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">420</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">14</span> Changes in Heavy Metals Bioavailability in Manure-Derived Digestates and Subsequent Hydrochars to Be Used as Soil Amendments</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hellen%20L.%20De%20Castro%20e%20Silva">Hellen L. De Castro e Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Ana%20A.%20Robles%20Aguilar"> Ana A. Robles Aguilar</a>, <a href="https://publications.waset.org/abstracts/search?q=Erik%20Meers"> Erik Meers</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Digestates are residual by-products, rich in nutrients and trace elements, which can be used as organic fertilisers on soils. However, due to the non-digestibility of these elements and reduced dry matter during the anaerobic digestion process, metal concentrations are higher in digestates than in feedstocks, which might hamper their use as fertilisers according to the threshold values of some country policies. Furthermore, there is uncertainty regarding the required assimilated amount of these elements by some crops, which might result in their bioaccumulation. Therefore, further processing of the digestate to obtain safe fertilizing products has been recommended. This research aims to analyze the effect of applying the hydrothermal carbonization process to manure-derived digestates as a thermal treatment to reduce the bioavailability of heavy metals in mono and co-digestates derived from pig manure and maize from contaminated land in France. This study examined pig manure collected from a novel stable system (VeDoWs, province of East Flanders, Belgium) that separates the collection of pig urine and feces, resulting in a solid fraction of manure with high up-concentration of heavy metals and nutrients. Mono-digestion and co-digestion processes were conducted in semi-continuous reactors for 45 days at mesophilic conditions, in which the digestates were dried at 105 °C for 24 hours. Then, hydrothermal carbonization was applied to a 1:10 solid/water ratio to guarantee controlled experimental conditions in different temperatures (180, 200, and 220 °C) and residence times (2 h and 4 h). During the process, the pressure was generated autogenously, and the reactor was cooled down after completing the treatments. The solid and liquid phases were separated through vacuum filtration, in which the solid phase of each treatment -hydrochar- was dried and ground for chemical characterization. Different fractions (exchangeable / adsorbed fraction - F1, carbonates-bound fraction - F2, organic matter-bound fraction - F3, and residual fraction – F4) of some heavy metals (Cd, Cr, Ni, and Cr) have been determined in digestates and derived hydrochars using the modified Community Bureau of Reference (BCR) sequential extraction procedure. The main results indicated a difference in the heavy metals fractionation between digestates and their derived hydrochars; however, the hydrothermal carbonization operating conditions didn’t have remarkable effects on heavy metals partitioning between the hydrochars of the proposed treatments. Based on the estimated potential ecological risk assessment, there was one level decrease (considerate to moderate) when comparing the HMs partitioning in digestates and derived hydrochars. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=heavy%20metals" title="heavy metals">heavy metals</a>, <a href="https://publications.waset.org/abstracts/search?q=bioavailability" title=" bioavailability"> bioavailability</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrothermal%20treatment" title=" hydrothermal treatment"> hydrothermal treatment</a>, <a href="https://publications.waset.org/abstracts/search?q=bio-based%20fertilisers" title=" bio-based fertilisers"> bio-based fertilisers</a>, <a href="https://publications.waset.org/abstracts/search?q=agriculture" title=" agriculture"> agriculture</a> </p> <a href="https://publications.waset.org/abstracts/157604/changes-in-heavy-metals-bioavailability-in-manure-derived-digestates-and-subsequent-hydrochars-to-be-used-as-soil-amendments" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/157604.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">100</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> Gold Nano Particle as a Colorimetric Sensor of HbA0 Glycation Products</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ranjita%20Ghoshmoulick">Ranjita Ghoshmoulick</a>, <a href="https://publications.waset.org/abstracts/search?q=Aswathi%20Madhavan"> Aswathi Madhavan</a>, <a href="https://publications.waset.org/abstracts/search?q=Subhavna%20Juneja"> Subhavna Juneja</a>, <a href="https://publications.waset.org/abstracts/search?q=Prasenjit%20Sen"> Prasenjit Sen</a>, <a href="https://publications.waset.org/abstracts/search?q=Jaydeep%20Bhattacharya"> Jaydeep Bhattacharya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Type 2 diabetes mellitus (T2DM) is a very complex and multifactorial metabolic disease where the blood sugar level goes up. One of the major consequence of this elevated blood sugar is the formation of AGE (Advance Glycation Endproducts), from a series of chemical or biochemical reactions. AGE are detrimental because it leads to severe pathogenic complications. They are a group of structurally diverse chemical compounds formed from nonenzymatic reactions between the free amino groups (-NH2) of proteins and carbonyl groups (>C=O) of reducing sugars. The reaction is known as Maillard Reaction. It starts with the formation of reversible schiff’s base linkage which after sometime rearranges itself to form Amadori Product along with dicarbonyl compounds. Amadori products are very unstable hence rearrangement goes on until stable products are formed. During the course of the reaction a lot of chemically unknown intermediates and reactive byproducts are formed that can be termed as Early Glycation Products. And when the reaction completes, structurally stable chemical compounds are formed which is termed as Advanced Glycation Endproducts. Though all glycation products have not been characterized well, some fluorescence compounds e.g pentosidine, Malondialdehyde (MDA) or carboxymethyllysine (CML) etc as AGE and α-dicarbonyls or oxoaldehydes such as 3-deoxyglucosone (3-DG) etc as the intermediates have been identified. In this work Gold NanoParticle (GNP) was used as an optical indicator of glycation products. To achieve faster glycation kinetics and high AGE accumulation, fructose was used instead of glucose. Hemoglobin A0 (HbA0) was fructosylated by in-vitro method. AGE formation was measured fluorimetrically by recording emission at 450nm upon excitation at 350nm. Thereafter this fructosylated HbA0 was fractionated by column chromatography. Fractionation separated the proteinaceous substance from the AGEs. Presence of protein part in the fractions was confirmed by measuring the intrinsic protein fluorescence and Bradford reaction. GNPs were synthesized using the templates of chromatographically separated fractions of fructosylated HbA0. Each fractions gave rise to GNPs of varying color, indicating the presence of distinct set of glycation products differing structurally and chemically. Clear solution appeared due to settling down of particles in some vials. The reactive groups of the intermediates kept the GNP formation mechanism on and did not lead to a stable particle formation till Day 10. Whereas SPR of GNP showed monotonous colour for the fractions collected in case of non fructosylated HbA0. Our findings accentuate the use of GNPs as a simple colorimetric sensing platform for the identification of intermediates of glycation reaction which could be implicated in the prognosis of the associated health risk due to T2DM and others. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=advance%20glycation%20endproducts" title="advance glycation endproducts">advance glycation endproducts</a>, <a href="https://publications.waset.org/abstracts/search?q=glycation" title=" glycation"> glycation</a>, <a href="https://publications.waset.org/abstracts/search?q=gold%20nano%20particle" title=" gold nano particle"> gold nano particle</a>, <a href="https://publications.waset.org/abstracts/search?q=sensor" title=" sensor"> sensor</a> </p> <a href="https://publications.waset.org/abstracts/63738/gold-nano-particle-as-a-colorimetric-sensor-of-hba0-glycation-products" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63738.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">303</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> In vivo Wound Healing Activity and Phytochemical Screening of the Crude Extract and Various Fractions of Kalanchoe petitiana A. Rich (Crassulaceae) Leaves in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Awol%20Mekonnen">Awol Mekonnen</a>, <a href="https://publications.waset.org/abstracts/search?q=Temesgen%20Sidamo"> Temesgen Sidamo</a>, <a href="https://publications.waset.org/abstracts/search?q=Epherm%20Engdawork"> Epherm Engdawork</a>, <a href="https://publications.waset.org/abstracts/search?q=Kaleab%20Asresb"> Kaleab Asresb</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ethnopharmacological Relevance: The leaves of Kalanchoe petitiana A. Rich (Crassulaceae) are used in Ethiopian folk medicine for treatment of evil eye, fractured surface for bone setting and several skin disorders including for the treatment of sores, boils, and malignant wounds. Aim of the Study: In order to scientifically prove the claimed utilization of the plant, the effects of the extracts and the fractions were investigated using in vivo excision, incision and dead space wound models. Materials and Method: Mice were used for wound healing study, while rats and rabbit were used for skin irritation test. For studying healing activity, 80% methanolic extract and the fractions were formulated in strength of 5% and 10%, either as ointment (hydroalcoholic extract, aqueous and methanol fractions) or gel (chloroform fraction). Oral administration of the crude extract was used for dead space model. Negative controls were treated either with simple ointment or sodium carboxyl methyl cellulose xerogel, while positive controls were treated with nitrofurazone (0.2 w/v) skin ointment. Negative controls for dead space model were treated with 1% carboxy methyl cellulose. Parameters, including rate of wound contraction, period of complete epithelializtion, hydroxyproline contents and skin breaking strength were evaluated. Results: Significant wound healing activity was observed with ointment formulated from the crude extract at both 5% and 10% concentration (p<0.01) compared to controls in both excision and incision models. In dead space model, 600 mg/kg (p<0.01), but not 300 mg/kg, significantly increased hydroxyproline content. Fractions showed variable effect, with the chloroform fraction lacking any significant effect. Both 5% and 10% formulations of the aqueous and methanolic fractions significantly increased wound contraction, decreased epithelializtion time and increased hydroxyproline content in excision wound model (p<0.05) as compared to controls. These fractions were also endowed with higher skin breaking strength in incision wound model (p<0.01). Conclusions: The present study provided evidence that the leaves of Kalanchoe petitiana A. Rich possess remarkable wound healing activities supporting the folkloric assertion of the plant. Fractionation revealed that polar or semi-polar compound may play vital role, as both aqueous and methanolic fractions were endowed with wound healing activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=wound%20healing" title="wound healing">wound healing</a>, <a href="https://publications.waset.org/abstracts/search?q=Kalanchoae%20petitiana" title=" Kalanchoae petitiana"> Kalanchoae petitiana</a>, <a href="https://publications.waset.org/abstracts/search?q=excision%20wound" title=" excision wound"> excision wound</a>, <a href="https://publications.waset.org/abstracts/search?q=incision%20wound" title=" incision wound"> incision wound</a>, <a href="https://publications.waset.org/abstracts/search?q=dead%20space%20model" title=" dead space model"> dead space model</a> </p> <a href="https://publications.waset.org/abstracts/1676/in-vivo-wound-healing-activity-and-phytochemical-screening-of-the-crude-extract-and-various-fractions-of-kalanchoe-petitiana-a-rich-crassulaceae-leaves-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/1676.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">309</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">11</span> Organic Matter Distribution in Bazhenov Source Rock: Insights from Sequential Extraction and Molecular Geochemistry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Margarita%20S.%20Tikhonova">Margarita S. Tikhonova</a>, <a href="https://publications.waset.org/abstracts/search?q=Alireza%20Baniasad"> Alireza Baniasad</a>, <a href="https://publications.waset.org/abstracts/search?q=Anton%20G.%20Kalmykov"> Anton G. Kalmykov</a>, <a href="https://publications.waset.org/abstracts/search?q=Georgy%20A.%20Kalmykov"> Georgy A. Kalmykov</a>, <a href="https://publications.waset.org/abstracts/search?q=Ralf%20Littke"> Ralf Littke</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There is a high complexity in the pore structure of organic-rich rocks caused by the combination of inter-particle porosity from inorganic mineral matter and ultraﬁne intra-particle porosity from both organic matter and clay minerals. Fluids are retained in that pore space, but there are major uncertainties in how and where the fluids are stored and to what extent they are accessible or trapped in 'closed' pores. A large degree of tortuosity may lead to fractionation of organic matter so that the lighter and flexible compounds would diffuse to the reservoir whereas more complicated compounds may be locked in place. Additionally, parts of hydrocarbons could be bound to solid organic matter –kerogen– and mineral matrix during expulsion and migration. Larger compounds can occupy thin channels so that clogging or oil and gas entrapment will occur. Sequential extraction of applying different solvents is a powerful tool to provide more information about the characteristics of trapped organic matter distribution. The Upper Jurassic – Lower Cretaceous Bazhenov shale is one of the most petroliferous source rock extended in West Siberia, Russia. Concerning the variable mineral composition, pore space distribution and thermal maturation, there are high uncertainties in distribution and composition of organic matter in this formation. In order to address this issue geological and geochemical properties of 30 samples including mineral composition (XRD and XRF), structure and texture (thin-section microscopy), organic matter contents, type and thermal maturity (Rock-Eval) as well as molecular composition (GC-FID and GC-MS) of different extracted materials during sequential extraction were considered. Sequential extraction was performed by a Soxhlet apparatus using different solvents, i.e., n-hexane, chloroform and ethanol-benzene (1:1 v:v) first on core plugs and later on pulverized materials. The results indicate that the studied samples are mainly composed of type II kerogen with TOC contents varied from 5 to 25%. The thermal maturity ranged from immature to late oil window. Whereas clay contents decreased with increasing maturity, the amount of silica increased in the studied samples. According to molecular geochemistry, stored hydrocarbons in open and closed pore space reveal different geochemical fingerprints. The results improve our understanding of hydrocarbon expulsion and migration in the organic-rich Bazhenov shale and therefore better estimation of hydrocarbon potential for this formation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bazhenov%20formation" title="Bazhenov formation">Bazhenov formation</a>, <a href="https://publications.waset.org/abstracts/search?q=bitumen" title=" bitumen"> bitumen</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20geochemistry" title=" molecular geochemistry"> molecular geochemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=sequential%20extraction" title=" sequential extraction"> sequential extraction</a> </p> <a href="https://publications.waset.org/abstracts/101704/organic-matter-distribution-in-bazhenov-source-rock-insights-from-sequential-extraction-and-molecular-geochemistry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/101704.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">170</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10</span> Characterization of Phenolic Compounds from Carménère Wines during Aging with Oak Wood (Staves, Chips and Barrels)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=E.%20Obreque-Slier">E. Obreque-Slier</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Laqui-Esta%C3%B1a"> J. Laqui-Estaña</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Pe%C3%B1a-Neira"> A. Peña-Neira</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Medel-Marabol%C3%AD"> M. Medel-Marabolí</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Wine is an important source of polyphenols. Red wines show important concentrations of nonflavonoid (gallic acid, ellagic acid, caffeic acid and coumaric acid) and flavonoid compounds [(+)-catechin, (-)-epicatechin, (+)-gallocatechin and (-)-epigallocatechin]. However, a significant variability in the quantitative and qualitative distribution of chemical constituents in wine has to be expected depending on an array of important factors, such as the varietal differences of Vitis vinifera and cultural practices. It has observed that Carménère grapes present a differential composition and evolution of phenolic compounds when compared to other varieties and specifically with Cabernet Sauvignon grapes. Likewise, among the cultural practices, the aging in contact with oak wood is a high relevance factor. Then, the extraction of different polyphenolic compounds from oak wood into wine during its ageing process produces both qualitative and quantitative changes. Recently, many new techniques have been introduced in winemaking. One of these involves putting new pieces of wood (oak chips or inner staves) into inert containers. It offers some distinct and previously unavailable ﬂavour advantages, as well as new options in wine handling. To our best knowledge, there is not information about the behaviour of Carménère wines (Chilean emblematic cultivar) in contact with oak wood. In addition, the effect of aging time and wood product (barrels, chips or staves) on the phenolic composition in Carménère wines has not been studied. This study aims at characterizing the condensed and hydrolyzable tannins from Carménère wines during the aging with staves, chips and barrels from French oak wood. The experimental design was completely randomized with two independent assays: aging time (0-12 month) and different formats of wood (barrel, chips and staves). The wines were characterized by spectrophotometric (total tannins and fractionation of proanthocyanidins into monomers, oligomers and polymers) and HPLC-DAD (ellagitannins) analysis. The wines in contact with different products of oak wood showed a similar content of total tannins during the study, while the control wine (without oak wood) presented a lower content of these compounds. In addition, it was observed that the polymeric proanthocyanidin fraction was the most abundant, while the monomeric fraction was the less abundant fraction in all treatments in two sample. However, significative differences in each fractions were observed between wines in contact from barrel, chips, and staves in two sample dates. Finally, the wine from barrels presented the highest content of the ellagitannins from the fourth to the last sample date. In conclusion, the use of alternative formats of oak wood affects the chemical composition of wines during aging, and these enological products are an interesting alternative to contribute with tannins to wine. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=enological%20inputs" title="enological inputs">enological inputs</a>, <a href="https://publications.waset.org/abstracts/search?q=oak%20wood%20aging" title=" oak wood aging"> oak wood aging</a>, <a href="https://publications.waset.org/abstracts/search?q=polyphenols" title=" polyphenols"> polyphenols</a>, <a href="https://publications.waset.org/abstracts/search?q=red%20wine" title=" red wine"> red wine</a> </p> <a href="https://publications.waset.org/abstracts/75826/characterization-of-phenolic-compounds-from-carmenere-wines-during-aging-with-oak-wood-staves-chips-and-barrels" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75826.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">158</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Processes Controlling Release of Phosphorus (P) from Catchment Soils and the Relationship between Total Phosphorus (TP) and Humic Substances (HS) in Scottish Loch Waters</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xiaoyun%20Hui">Xiaoyun Hui</a>, <a href="https://publications.waset.org/abstracts/search?q=Fiona%20Gentle"> Fiona Gentle</a>, <a href="https://publications.waset.org/abstracts/search?q=Clemens%20Engelke"> Clemens Engelke</a>, <a href="https://publications.waset.org/abstracts/search?q=Margaret%20C.%20Graham"> Margaret C. Graham</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Although past work has shown that phosphorus (P), an important nutrient, may form complexes with aqueous humic substances (HS), the principal component of natural organic matter, the nature of such interactions is poorly understood. Humic complexation may not only enhance P concentrations but it may change its bioavailability within such waters and, in addition, influence its transport within catchment settings. This project is examining the relationships and associations of P, HS, and iron (Fe) in Loch Meadie, Sutherland, North Scotland, a mesohumic freshwater loch which has been assessed as reference condition with respect to P. The aim is to identify characteristic spectroscopic parameters which can enhance the performance of the model currently used to predict reference condition TP levels for highly-coloured Scottish lochs under the Water Framework Directive. In addition to Loch Meadie, samples from other reference condition lochs in north Scotland and Shetland were analysed. By including different types of reference condition lochs (clear water, mesohumic and polyhumic water) this allowed the relationship between total phosphorus (TP) and HS to be more fully explored. The pH, [TP], [Fe], UV/Vis absorbance/spectra, [TOC] and [DOC] for loch water samples have been obtained using accredited methods. Loch waters were neutral to slightly acidic/alkaline (pH 6-8). [TP] in loch waters were lower than 50 µg L-1, and in Loch Meadie waters were typically <10 µg L-1. [Fe] in loch waters were mainly <0.6 mg L-1, but for some loch water samples, [Fe] were in the range 1.0-1.8 mg L-1and there was a positive correlation with [TOC] (r2=0.61). Lochs were classified as clear water, mesohumic or polyhumic based on water colour. The range of colour values of sampled lochs in each category were 0.2–0.3, 0.2–0.5 and 0.5–0.8 a.u. (10 mm pathlength), respectively. There was also a strong positive correlation between [DOC] and water colour (R2=0.84). The UV/Vis spectra (200-700 nm) for water samples were featureless with only a slight “shoulder” observed in the 270–290 nm region. Ultrafiltration was then used to separate colloidal and truly dissolved components from the loch waters and, since it contained the majority of aqueous P and Fe, the colloidal component was fractionated by gel filtration chromatography method. Gel filtration chromatographic fractionation of the colloids revealed two brown-coloured bands which had distinctive UV/Vis spectral features. The first eluting band had larger and more aromatic HS molecules than the second band, and in addition both P and Fe were primarily associated with the larger, more aromatic HS. This result demonstrated that P was able to form complexes with Fe-rich components of HS, and thus provided a scientific basis for the significant correlation between [Fe] and [TP] that the previous monitoring data of reference condition lochs from Scottish Environment Protection Agency (SEPA) showed. The distinctive features of the HS will be used as the basis for an improved spectroscopic tool. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=total%20phosphorus" title="total phosphorus">total phosphorus</a>, <a href="https://publications.waset.org/abstracts/search?q=humic%20substances" title=" humic substances"> humic substances</a>, <a href="https://publications.waset.org/abstracts/search?q=Scottish%20loch%20water" title=" Scottish loch water"> Scottish loch water</a>, <a href="https://publications.waset.org/abstracts/search?q=WFD%20model" title=" WFD model "> WFD model </a> </p> <a href="https://publications.waset.org/abstracts/18131/processes-controlling-release-of-phosphorus-p-from-catchment-soils-and-the-relationship-between-total-phosphorus-tp-and-humic-substances-hs-in-scottish-loch-waters" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/18131.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">546</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Metal Contents in Bird Feathers (Columba livia) from Mt Etna Volcano: Volcanic Plume Contribution and Biological Fractionation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Edda%20E.%20Falcone">Edda E. Falcone</a>, <a href="https://publications.waset.org/abstracts/search?q=Cinzia%20Federico"> Cinzia Federico</a>, <a href="https://publications.waset.org/abstracts/search?q=Sergio%20Bellomo"> Sergio Bellomo</a>, <a href="https://publications.waset.org/abstracts/search?q=Lorenzo%20Brusca"> Lorenzo Brusca</a>, <a href="https://publications.waset.org/abstracts/search?q=Manfredi%20Longo"> Manfredi Longo</a>, <a href="https://publications.waset.org/abstracts/search?q=Walter%20D%E2%80%99Alessandro"> Walter D’Alessandro</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Although trace metals are an essential element for living beings, they can become toxic at high concentrations. Their potential toxicity is related not only to the total content in the environment but mostly upon their bioavailability. Volcanoes are important natural metal emitters and they can deeply affect the quality of air, water and soils, as well as the human health. Trace metals tend to accumulate in the tissues of living organisms, depending on the metal contents in food, air and water and on the exposure time. Birds are considered as bioindicators of interest, because their feathers directly reflects the metals uptake from the blood. Birds are exposed to the atmospheric pollution through the contact with rainfall, dust, and aerosol, and they accumulate metals over the whole life cycle. We report on the first data combining the rainfall metal content in three different areas of Mt Etna, variably fumigated by the volcanic plume, and the metal contents in the feathers of pigeons, collected in the same areas. Rainfall samples were collected from three rain gauges placed at different elevation on the Eastern flank of the volcano, the most exposed to airborne plume, filtered, treated with HNO₃ Suprapur-grade and analyzed for Fe, Cr, Co, Ni, Se, Zn, Cu, Sr, Ba, Cd and As by ICP-MS technique, and major ions by ion chromatography. Feathers were collected from single individuals, in the same areas where the rain gauges were installed. Additionally, some samples were collected in an urban area, poorly interested by the volcanic plume. The samples were rinsed in MilliQ water and acetone, dried at 50°C until constant weight and digested in a mixture of 2:1 HNO₃ (65%) - H₂O₂ (30%) Suprapur-grade for 25-50 mg of sample, in a bath at near-to-boiling temperature. The solutions were diluted up to 20 ml prior to be analyzed by ICP-MS. The rainfall samples most contaminated by the plume were collected at close distance from the summit craters (less than 6 km), and show lower pH values and higher concentrations for all analyzed metals relative to those from the sites at lower elevation. Analyzed samples are enriched in both metals directly emitted by the volcanic plume and transported by acidic gases (SO₂, HCl, HF), and metals leached from the airborne volcanic ash. Feathers show different patterns in the different sites related to the exposure to natural or anthropogenic pollutants. They show abundance ratios similar to rainfall for lithophile elements (Ba, Sr), whereas are enriched in Zn and Se, known for their antioxidant properties, probably as adaptive response to oxidative stress induced by toxic metal exposure. The pigeons revealed a clear heterogeneity of metal uptake in the different parts of the volcano, as an effect of volcanic plume impact. Additionally, some physiological processes can modify the fate of some metals after uptake and this offer some insights for translational studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioindicators" title="bioindicators">bioindicators</a>, <a href="https://publications.waset.org/abstracts/search?q=environmental%20pollution" title=" environmental pollution"> environmental pollution</a>, <a href="https://publications.waset.org/abstracts/search?q=feathers" title=" feathers"> feathers</a>, <a href="https://publications.waset.org/abstracts/search?q=trace%20metals" title=" trace metals"> trace metals</a>, <a href="https://publications.waset.org/abstracts/search?q=volcanic%20plume" title=" volcanic plume"> volcanic plume</a> </p> <a href="https://publications.waset.org/abstracts/101001/metal-contents-in-bird-feathers-columba-livia-from-mt-etna-volcano-volcanic-plume-contribution-and-biological-fractionation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/101001.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">143</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Altering the Solid Phase Speciation of Arsenic in Paddy Soil: An Approach to Reduce Rice Grain Arsenic Uptake </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Supriya%20Majumder">Supriya Majumder</a>, <a href="https://publications.waset.org/abstracts/search?q=Pabitra%20Banik"> Pabitra Banik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fates of Arsenic (As) on the soil-plant environment belong to the critical emerging issue, which in turn to appraises the threatening implications of a human health risk — assessing the dynamics of As in soil solid components are likely to impose its potential availability towards plant uptake. In the present context, we introduced an improved Sequential Extraction Procedure (SEP) questioning to identify solid-phase speciation of As in paddy soil under variable soil environmental conditions during two consecutive seasons of rice cultivation practices. We coupled gradients of water management practices with the addition of fertilizer amendments to assess the changes in a partition of As through a field experimental study during monsoon and post-monsoon season using two rice cultivars. Water management regimes were varied based on the methods of cultivation of rice by Conventional (waterlogged) vis-a-vis System of Rice Intensification-SRI (saturated). Fertilizer amendment through the nutrient treatment of absolute control, NPK-RD, NPK-RD + Calcium silicate, NPK-RD + Ferrous sulfate, Farmyard manure (FYM), FYM + Calcium silicate, FYM + Ferrous sulfate, Vermicompost (VC), VC + Calcium silicate, VC + Ferrous sulfate were selected to construct the study. After harvest, soil samples were sequentially extracted to estimate partition of As among the different fractions such as: exchangeable (F1), specifically sorbed (F2), As bound to amorphous Fe oxides (F3), crystalline Fe oxides (F4), organic matter (F5) and residual phase (F6). Results showed that the major proportions of As were found in F3, F4 and F6, whereas F1 exhibited the lowest proportion of total soil As. Among the nutrient treatment mediated changes on As fractions, the application of organic manure and ferrous sulfate were significantly found to restrict the release of As from exchangeable phase. Meanwhile, conventional practice produced much higher release of As from F1 as compared to SRI, which may substantially increase the environmental risk. In contrast, SRI practice was found to retain a significantly higher proportion of As in F2, F3, and F4 phase resulting restricted mobilization of As. This was critically reflected towards rice grain As bioavailability where the reduction in grain As concentration of 33% and 55% in SRI concerning conventional treatment (p <0.05) during monsoon and post-monsoon season respectively. Also, prediction assay for rice grain As bioavailability based on the linear regression model was performed. Results demonstrated that rice grain As concentration was positively correlated with As concentration in F1 and negatively correlated with F2, F3, and F4 with a satisfactory level of variation being explained (p <0.001). Finally, we conclude that F1, F2, F3 and F4 are the major soil. As fractions critically may govern the potential availability of As in soil and suggest that rice cultivation with the SRI treatment is particularly at less risk of As availability in soil. Such exhaustive information may be useful for adopting certain management practices for rice grown in contaminated soil concerning to the environmental issues in particular. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=arsenic" title="arsenic">arsenic</a>, <a href="https://publications.waset.org/abstracts/search?q=fractionation" title=" fractionation"> fractionation</a>, <a href="https://publications.waset.org/abstracts/search?q=paddy%20soil" title=" paddy soil"> paddy soil</a>, <a href="https://publications.waset.org/abstracts/search?q=potential%20availability" title=" potential availability"> potential availability</a> </p> <a href="https://publications.waset.org/abstracts/122462/altering-the-solid-phase-speciation-of-arsenic-in-paddy-soil-an-approach-to-reduce-rice-grain-arsenic-uptake" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/122462.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">123</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Geochemical Evolution of Microgranular Enclaves Hosted in Cambro-Ordovician Kyrdem Granitoids, Meghalaya Plateau, Northeast India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20Mohon%20Singh">K. Mohon Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cambro-Ordovician (512.5 ± 8.7 Ma) felsic magmatism in the Kyrdem region of Meghalaya plateau, herewith referred to as Kyrdem granitoids (KG), intrudes the low-grade Shillong Group of metasediments and Precambrian Basement Gneissic complex forming an oval-shaped plutonic body with longer axis almost trending N-S. Thermal aureole is poorly developed or covered under the alluvium. KG exhibit very coarse grained porphyritic texture with abundant K-feldspar megacrysts (up to 9cm long) and subordinate amount of amphibole, biotite, plagioclase, and quartz. The size of K-feldspar megacrysts increases from margin (Dwarksuid) to the interior (Kyrdem) of the KG pluton. Late felsic pulses as fine grained granite, leucocratic (aplite), and pegmatite veins intrude the KG at several places. Grey and pink varieties of KG can be recognized, but pink colour of KG is the result of post-magmatic fluids, which have not affected the magnetic properties of KG. Modal composition of KG corresponds to quartz monzonite, monzogranite, and granodiorite. KG has been geochemically characterized as metaluminous (I-type) to peraluminous (S-type) granitoids. The KG is characterized by development of variable attitude of primary foliations mostly marked along the margin of the pluton and is located at the proximity of Tyrsad-Barapani lineament. The KG contains country rock xenoliths (amphibolite, gneiss, schist, etc.) which are mostly confined to the margin of the pluton, and microgranular enclaves (ME) are hosted in the porphyritic variety of KG. Microgranular Enclaves (ME) in Kyrdem Granitoids are fine- to medium grained, mesocratic to melanocratic, phenocryst bearing or phenocryst-free, rounded to ellipsoidal showing typical magmatic textures. Mafic-felsic phenocrysts in ME are partially corroded and dissolved because of their involvement in magma-mixing event, and thus represent xenocrysts. Sharp to diffused contacts of ME with host Kyrdem Granitoids, fine grained nature and presence of acicular apatite in ME suggest comingling and undercooling of coeval, semi-solidified ME magma within partly crystalline felsic host magma. Geochemical features recognize the nature of ME (molar A/CNK=0.76-1.42) and KG (molar A/CNK =0.41-1.75) similar to hybrid-type formed by mixing of mantle-derived mafic and crustal-derived felsic magmas. Major and trace including rare earth elements variations of ME suggest the involvement of combined processes such as magma mixing, mingling and crystallization differentiation in the evolution of ME but KG variations appear primarily controlled by fractionation of plagioclase, hornblende biotite, and accessory phases. Most ME are partially to nearly re-equilibrate chemically with felsic host KG during magma mixing and mingling processes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=geochemistry" title="geochemistry">geochemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=Kyrdem%20Granitoids" title=" Kyrdem Granitoids"> Kyrdem Granitoids</a>, <a href="https://publications.waset.org/abstracts/search?q=microgranular%20enclaves" title=" microgranular enclaves"> microgranular enclaves</a>, <a href="https://publications.waset.org/abstracts/search?q=Northeast%20India" title=" Northeast India"> Northeast India</a> </p> <a href="https://publications.waset.org/abstracts/107635/geochemical-evolution-of-microgranular-enclaves-hosted-in-cambro-ordovician-kyrdem-granitoids-meghalaya-plateau-northeast-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/107635.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">118</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Phenolic Acids of Plant Origin as Promising Compounds for Elaboration of Antiviral Drugs against Influenza</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vladimir%20Berezin">Vladimir Berezin</a>, <a href="https://publications.waset.org/abstracts/search?q=Aizhan%20Turmagambetova"> Aizhan Turmagambetova</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrey%20Bogoyavlenskiy"> Andrey Bogoyavlenskiy</a>, <a href="https://publications.waset.org/abstracts/search?q=Pavel%20Alexyuk"> Pavel Alexyuk</a>, <a href="https://publications.waset.org/abstracts/search?q=Madina%20Alexyuk"> Madina Alexyuk</a>, <a href="https://publications.waset.org/abstracts/search?q=Irina%20Zaitceva"> Irina Zaitceva</a>, <a href="https://publications.waset.org/abstracts/search?q=Nadezhda%20Sokolova"> Nadezhda Sokolova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Influenza viruses could infect approximately 5% to 10% of the global human population annually, resulting in serious social and economic damage. Vaccination and etiotropic antiviral drugs are used for the prevention and treatment of influenza. Vaccination is important; however, antiviral drugs represent the second line of defense against new emerging influenza virus strains for which vaccines may be unsuccessful. However, the significant drawback of commercial synthetic anti-flu drugs is the appearance of drug-resistant influenza virus strains. Therefore, the search and development of new anti-flu drugs efficient against drug-resistant strains is an important medical problem for today. The aim of this work was a study of four phenolic acids of plant origin (Gallic, Syringic, Vanillic, and Protocatechuic acids) as a possible tool for treatment against influenza virus. Methods: Phenolic acids; gallic, syringic, vanillic, and protocatechuic have been prepared by extraction from plant tissues and purified using high-performance liquid chromatography fractionation. Avian influenza virus, strain A/Tern/South Africa/1/1961 (H5N3) and human epidemic influenza virus, strain A/Almaty/8/98 (H3N2) resistant to commercial anti-flu drugs (Rimantadine, Oseltamivir) were used for testing antiviral activity. Viruses were grown in the allantoic cavity of 10 days old chicken embryos. The chemotherapeutic index (CTI), determined as the ratio of an average toxic concentration of the tested compound (TC₅₀) to the average effective virus-inhibition concentration (EC₅₀), has been used as a criteria of specific antiviral action. Results: The results of study have shown that the structure of phenolic acids significantly affected their ability to suppress the reproduction of tested influenza virus strains. The highest antiviral activity among tested phenolic acids was detected for gallic acid, which contains three hydroxyl groups in the molecule at C3, C4, and C5 positions. Antiviral activity of gallic acid against A/H5N3 and A/H3N2 influenza virus strains was higher than antiviral activity of Oseltamivir and Rimantadine. gallic acid inhibited almost 100% of the infection activity of both tested viruses. Protocatechuic acid, which possesses 2 hydroxyl groups (C3 and C4) have shown weaker antiviral activity in comparison with gallic acid and inhibited less than 10% of virus infection activity. Syringic acid, which contains two hydroxyl groups (C3 and C5), was able to suppress up to 12% of infection activity. Substitution of two hydroxyl groups by methoxy groups resulted in the complete loss of antiviral activity. Vanillic acid, which is different from protocatechuic acid by replacing of C3 hydroxyl group to methoxy group, was able to suppress about 30% of infection activity of tested influenza viruses. Conclusion: For pronounced antiviral activity, the molecular of phenolic acid must have at least two hydroxyl groups. Replacement of hydroxyl groups to methoxy group leads to a reduction of antiviral properties. Gallic acid demonstrated high antiviral activity against influenza viruses, including Rimantadine and Oseltamivir resistant strains, and could be used as a potential candidate for the development of antiviral drug against influenza virus. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antiviral%20activity" title="antiviral activity">antiviral activity</a>, <a href="https://publications.waset.org/abstracts/search?q=influenza%20virus" title=" influenza virus"> influenza virus</a>, <a href="https://publications.waset.org/abstracts/search?q=drug%20resistance" title=" drug resistance"> drug resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolic%20acids" title=" phenolic acids"> phenolic acids</a> </p> <a href="https://publications.waset.org/abstracts/118980/phenolic-acids-of-plant-origin-as-promising-compounds-for-elaboration-of-antiviral-drugs-against-influenza" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/118980.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">141</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> A Rapid and Greener Analysis Approach Based on Carbonfiber Column System and MS Detection for Urine Metabolomic Study After Oral Administration of Food Supplements </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zakia%20Fatima">Zakia Fatima</a>, <a href="https://publications.waset.org/abstracts/search?q=Liu%20Lu"> Liu Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=Donghao%20Li"> Donghao Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The analysis of biological fluid metabolites holds significant importance in various areas, such as medical research, food science, and public health. Investigating the levels and distribution of nutrients and their metabolites in biological samples allows researchers and healthcare professionals to determine nutritional status, find hypovitaminosis or hypervitaminosis, and monitor the effectiveness of interventions such as dietary supplementation. Moreover, analysis of nutrient metabolites provides insight into their metabolism, bioavailability, and physiological processes, aiding in the clarification of their health roles. Hence, the exploration of a distinct, efficient, eco-friendly, and simpler methodology is of great importance to evaluate the metabolic content of complex biological samples. In this work, a green and rapid analytical method based on an automated online two-dimensional microscale carbon fiber/activated carbon fiber fractionation system and time-of-flight mass spectrometry (2DμCFs-TOF-MS) was used to evaluate metabolites of urine samples after oral administration of food supplements. The automated 2DμCFs instrument consisted of a microcolumn system with bare carbon fibers and modified carbon fiber coatings. Carbon fibers and modified carbon fibers exhibit different surface characteristics and retain different compounds accordingly. Three kinds of mobile-phase solvents were used to elute the compounds of varied chemical heterogeneities. The 2DμCFs separation system has the ability to effectively separate different compounds based on their polarity and solubility characteristics. No complicated sample preparation method was used prior to analysis, which makes the strategy more eco-friendly, practical, and faster than traditional analysis methods. For optimum analysis results, mobile phase composition, flow rate, and sample diluent were optimized. Water-soluble vitamins, fat-soluble vitamins, and amino acids, as well as 22 vitamin metabolites and 11 vitamin metabolic pathway-related metabolites, were found in urine samples. All water-soluble vitamins except vitamin B12 and vitamin B9 were detected in urine samples. However, no fat-soluble vitamin was detected, and only one metabolite of Vitamin A was found. The comparison with a blank urine sample showed a considerable difference in metabolite content. For example, vitamin metabolites and three related metabolites were not detected in blank urine. The complete single-run screening was carried out in 5.5 minutes with the minimum consumption of toxic organic solvent (0.5 ml). The analytical method was evaluated in terms of greenness, with an analytical greenness (AGREE) score of 0.72. The method’s practicality has been investigated using the Blue Applicability Grade Index (BAGI) tool, obtaining a score of 77. The findings in this work illustrated that the 2DµCFs-TOF-MS approach could emerge as a fast, sustainable, practical, high-throughput, and promising analytical tool for screening and accurate detection of various metabolites, pharmaceuticals, and ingredients in dietary supplements as well as biological fluids. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=metabolite%20analysis" title="metabolite analysis">metabolite analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=sustainability" title=" sustainability"> sustainability</a>, <a href="https://publications.waset.org/abstracts/search?q=carbon%20fibers" title=" carbon fibers"> carbon fibers</a>, <a href="https://publications.waset.org/abstracts/search?q=urine." title=" urine."> urine.</a> </p> <a href="https://publications.waset.org/abstracts/188418/a-rapid-and-greener-analysis-approach-based-on-carbonfiber-column-system-and-ms-detection-for-urine-metabolomic-study-after-oral-administration-of-food-supplements" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188418.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">27</span> </span> </div> </div> <ul class="pagination"> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=NGL%20fractionation&page=2" rel="prev">&lsaquo;</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=NGL%20fractionation&page=1">1</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=NGL%20fractionation&page=2">2</a></li> <li class="page-item 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