ACA CryoEM Workshop 2019 : EMDataResource

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src="https://www.aca2019mtg.com/assets/site/amcac.png" alt="ACA2019" width="250px" /></a> <h2>2019 ACA Cryo-EM Workshop</h2> <p> The <b>Workshop on Biological Structure Determination using Cryo-EM</b> was held on July 22-23, 2019 as part of the <a href="https://www.aca2019mtg.com/">American Crystallographic Association Annual Meeting</a> in Covington, Kentucky USA. Co-organized by Cathy Lawson (Rutgers U), Wen Jiang (Purdue U), and Michael Cianfrocco (U Michigan) and sponsored by ThermoFisher Scientific, Electron Microscopy Sciences, MiTeGen, and Amazon Web Services, the two-afternoon workshop had over 80 registered attendees. This was the 2nd year that ACA's <a href="https://www.amercrystalassn.org/scientific-interest-groups" target="_blank">Cryo-EM SIG</a> has offered a workshop on this topic (see <a href="http://www-test.emdataresource.org/news/aca_workshop_2018.html" target="_blank">2018 workshop page</a>).</p>  <p>Cryo-EM is rapidly becoming the method of choice for structure determination of proteins larger than ~100 kDa, including membrane proteins, large assemblies and multi-protein complexes. Cryo-EM instrumentation is being installed in many universities, and is also available at large multiuser facilities both in the U.S. and worldwide. This workshop, which was held on two consecutive afternoons during the ACA 2019 main meeting (Mon. and Tues.), introduced scientists with crystallographic backgrounds to procedures used cryo-EM.</p> <p>Lectures focused on the steps involved in producing a cryo-EM map, from specimen preparation and data collection through to image processing and reconstruction. Aspects of workshop were hands-on: state-the-art programs were used by the students to process sample datasets.</p> <p>This workshop was intended benefit those who want to use cryo-EM in the future, or who have recently transitioned into it, with up-to-date discussions of cutting-edge methods and technology.</p> <p>Materials for the workshop can be accessed <a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AADvj6EmeFlYYxUedXYp8bUoa?dl=0" target="_blank">HERE(Dropbox)</a> or <a href="ftp://model-compare.emdataresource.org/ACAworkshop2019/" target="_blank">HERE(ftp)</a> and through the links below.</p> <br/> <h2>Preparation</h2> <p>For the particle picking and reconstruction tutorials, each student was given access to their own GPU computing node on the AWS cloud, as well as a temporary <a href="http://cryosparc.com">CryoSparc</a> license, and was requested to install <a href="https://www.cgl.ucsf.edu/chimera/">UCSF Chimera</a> in advance of the workshop.</p> <br/> <br/> <h2>Agenda</h2> <b>Day 1: July 22, 2019</b> <table class="table"> <tbody> <tr> <td width="10%"><b>Time</b></td> <td width="30%"><b>Instructor</b></td> <td width="60%"><b>Topic</b></td> </tr> <tr> <td>13:30 </td> <td>Cathy Lawson </td> <td>Workshop Introduction</td></tr> <tr> <td>13:35 </td> <td>Edward Eng </td> <td><a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AADK-8B9arktwHmqG9a7SWTNa/ACA2019_cryoEM_Workshop_Eng_final.pdf?dl=0" target="_blank"-->Lecture: What to Expect from Cryo-EM (National Service Centers)</a></td></tr> <tr> <td>14:00 </td> <td>Wen Jiang </td> <td><a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AABJqRCtz2x6_MkDWeru1vvUa/ACA2019_cryoEM_Workshop_Jiang_1_image_formation.pdf?dl=0" target="_blank">Lecture: Cryo-EM Image Formation</a></td></tr> <tr> <td>14:45 </td> <td>Jeff Lengyel </td> <td>The Latest and Greatest in CryoEM Technology</td></tr> <tr> <td>15:00 </td> <td> </td> <td>Coffee Break</td></tr> <tr> <td>15:15 </td> <td>Leifu Chang </td> <td><a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AAAx9yyhto-GQTJs-ZEWa3Dca/ACA2019_cryoEM_Workshop_Chang_1_sample_prep.pdf?dl=0" target="_blank">Lecture: Grid Preparation and Data Collection for Cryo-EM</a></td></tr> <tr> <td>16:15 </td> <td>Brenda Gonzalez </td> <td><a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AAByQwTpLdl2zAXhn7YWUlJAa/ACA2019_cryoEM_Workshop_CryoVR_final.pptx?dl=0" target="_blank">CryoVR: Virtual Reality Training & Outreach Tools for Cryo-EM</a></td></tr> <tr> <td>16:45 </td> <td>Michael Cianfrocco </td> <td><a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AABzb7gdtBP0t5CYpbn4n4Lxa/ACA2019_cryoEM_Workshop_AWS_intro_slides.pdf?dl=0" target="_blank">Preparation for Day 2 Demo</a></td></tr> </tbody> </table> <br/> <b>Day 2: July 23, 2019</b> <table class="table" > <tbody> <tr> <td width="10%"><b>Time</b></td> <td width="30%"><b>Instructor</b></td> <td width="60%"><b>Topic</b></td> </tr> <tr> <td>13:30 </td> <td>Wen Jiang </td> <td><a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AABf-pkB6ALZ_Fhj9gZQEN-pa/ACA2019_cryoEM_Workshop_Jiang_2_image%20processing.pdf?dl=0" target="_blank">Lecture: Image Processing & 3D Reconstruction</a></td></tr> <tr> <td>14:35 </td> <td>Michael Cianfrocco </td> <td><a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AADp6RM0KyixACs7_1_tG0e8a/ACA2019_cryoEM_Workshop_Cianfrocco.pdf?dl=0" target="_blank">Lecture: Cloud Computing Tools for Cryo-EM</a></td></tr> <tr> <td>15:00 </td> <td> </td> <td>Coffee Break</td></tr> <tr> <td>15:15 </td> <td>Michael Cianfrocco </td> <td>Demo: Cloud Computing Tools for Cryo-EM</td></tr> <tr> <td>16:00 </td> <td>Leifu Chang </td> <td><a href="https://www.dropbox.com/sh/sftw3w3b7l2cf3m/AADHYrOuaIwWNQ4BZ93rWbsFa/ACA2019_cryoEM_Workshop_Chang_2_difficult_specimens.pdf?dl=0" target="_blank">Lecture: Strategies for Difficult Specimens</a></td></tr> <tr> <td>16:30 </td> <td>Michael Cianfrocco </td> <td>Demo: Cloud Computing Tools for Cryo-EM wrap-up</td></tr> </tbody> </table> <br/> <p>updated 23 Jul 2019</p> <br class="clearfloat" /> </div> <footer class="page-footer"> <div class="container-fluid"> <div class="row" style="color:#666; 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ACA CryoEM Workshop 2019 : EMDataResource