Luminex Metabolic Panel Assess 12 Metabolic Biomarkers| Bio-Techne

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container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/reagents">Bio-Techne Reagents</a> </li> <li> <a href="/reagents/luminex-assays">Luminex Assays</a> </li> <li> Luminex Metabolic High Performance Panel </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Introducing the R&D Systems™ Luminex® Metabolic Performance Panel</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>To assist investigators, R&D Systems, a Bio-Techne brand has developed the Human Metabolic Luminex Panel (<a href="/p/luminex-assays/human-metabolic-luminex-performance-premixed-kit_fcstm19" target="_self">Catalog # FCSTM19</a>). This flexible, premixed, panel can be configured to quantify up to 12 metabolic biomarkers per 50uL sample in less than a day, which is faster than the competition. The Luminex metabolic panel saves time and precious sample, thus expediting your workflow. </p></div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-what-is-metabolic-syndrome4" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What is Metabolic Syndrome?</h5> </a> </div> <div class="collapse" id="expPara-what-is-metabolic-syndrome4" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Metabolic Syndrome describes a cluster of metabolic abnormalities that includes hypertension, central obesity, insulin resistance, and atherogenic dyslipidemia<sup>1</sup>. Whether these abnormalities emerge from common pathogenesis or not remains unclear. The etiology of Metabolic Syndrome includes lifestyle factors<sup>2</sup> as well as genetic and environmental factors. Metabolic Syndrome is associated with an increased risk for atherosclerotic cardiovascular disease and diabetes. It is apparent that Metabolic Syndrome involves the complex interplay of many metabolic biomarkers such as hormones and cytokines. Finally, the prevalence of Metabolic syndrome is trending upward, particularly in young adults<sup>3</sup>.  There was a statistically significant increase in the prevalence of metabolic syndrome in young adults ages 20-39 from 2011 to 2016. As such, metabolic biomarker research is required to understand its mechanisms so that the looming public health crisis can be averted.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/human-metabolic-luminex-panel-curves-525x525.jpg" alt="Luminex metabolic panel assay data showing concentration curve for 12 metabolic biomarkers" loading="lazy" > </div> </div> <div class="media-caption"> <div>Figure 1. Standards were serially diluted to generate a standard curve for each metabolic biomarker.</div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p> </p> <p> </p> <p><strong>Luminex Metabolic High Performance Features:</strong></p> <ul> <li>Measure up to 12 metabolic biomarkers simultaneously</li> <li>4.5 hour assay length, no overnight incubation</li> <li>Rigorous quality control testing for consistent and reliable data</li> <li>Small sample volume (50ul)</li> </ul> <p> </p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Metabolic biomarker sensitivity and precision is shown in Table 1. Assay precision includes the intra-assay coefficient of variation (CV) and inter-assay CV. The intra-assay CV and inter-assay CV are an estimate of variability within and between assays respectively. Assay CV should be less than 15%. As seen in table 1, the R&D Systems metabolic panel meets this precision standard, indicating that the panel is consistent and reproducible.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Table 1. List of metabolic biomarkers, sensitivity, and precision.</p> <figure class="responsive-figure-table"><table class="table table-bordered"> <tbody> <tr> <td> <h6>Metabolic Biomarker</h6> </td> <td> <h6><strong>Type of Analyte</strong></h6> </td> <td> <h6><strong>Sensitivity (pg/ml)</strong></h6> </td> <td> <h6><strong>Intra-assay CV (%)</strong></h6> </td> <td> <h6><strong>Inter-Assay CV (%)</strong></h6> </td> </tr> <tr> <td> <p><strong>Active GLP-1</strong></p> </td> <td> <p><strong>gut hormone</strong></p> </td> <td> <p><strong>0.833</strong></p> </td> <td> <p><strong>5</strong></p> </td> <td> <p><strong>9</strong></p> </td> </tr> <tr> <td> <p><strong>Active Peptide YY</strong></p> </td> <td> <p><strong>gut hormone</strong></p> </td> <td> <p><strong>1.39</strong></p> </td> <td> <p><strong>4</strong></p> </td> <td> <p><strong>8</strong></p> </td> </tr> <tr> <td> <p><strong>CCL2/MCP-1</strong></p> </td> <td> <p><strong>adipokine</strong></p> </td> <td> <p><strong>0.958</strong></p> </td> <td> <p><strong>3</strong></p> </td> <td> <p><strong>7</strong></p> </td> </tr> <tr> <td> <p><strong>C-Peptide</strong></p> </td> <td> <p><strong>pancreatic hormone</strong></p> </td> <td> <p><strong>0.578</strong></p> </td> <td> <p><strong>9</strong></p> </td> <td> <p><strong>13</strong></p> </td> </tr> <tr> <td> <p><strong>Glucagon</strong></p> </td> <td> <p><strong>pancreatic hormone</strong></p> </td> <td> <p><strong>4.43</strong></p> </td> <td> <p><strong>6</strong></p> </td> <td> <p><strong>11</strong></p> </td> </tr> <tr> <td> <p><strong>IL-6</strong></p> </td> <td> <p><strong>adipokine</strong></p> </td> <td> <p><strong>0.227</strong></p> </td> <td> <p><strong>4</strong></p> </td> <td> <p><strong>8</strong></p> </td> </tr> <tr> <td> <p><strong>IFN-Gamma</strong></p> </td> <td> <p><strong>cytokine</strong></p> </td> <td> <p><strong>0.072</strong></p> </td> <td> <p><strong>4</strong></p> </td> <td> <p><strong>8</strong></p> </td> </tr> <tr> <td> <p><strong>Insulin</strong></p> </td> <td> <p><strong>pancreatic hormone</strong></p> </td> <td> <p><strong>1.62</strong></p> </td> <td> <p><strong>6</strong></p> </td> <td> <p><strong>9</strong></p> </td> </tr> <tr> <td> <p><strong>Leptin</strong></p> </td> <td> <p><strong>adipokine</strong></p> </td> <td> <p><strong>22.2</strong></p> </td> <td> <p><strong>4</strong></p> </td> <td> <p><strong>11</strong></p> </td> </tr> <tr> <td> <p><strong>Pancreatic Polypeptide</strong></p> </td> <td> <p><strong>pancreatic hormone</strong></p> </td> <td> <p><strong>53.2</strong></p> </td> <td> <p><strong>9</strong></p> </td> <td> <p><strong>13</strong></p> </td> </tr> <tr> <td> <p><strong>TNF-Alpha</strong></p> </td> <td> <p><strong>adipokine</strong></p> </td> <td> <p><strong>0.141</strong></p> </td> <td> <p><strong>4</strong></p> </td> <td> <p><strong>9</strong></p> </td> </tr> <tr> <td> <p><strong>Total Ghrelin </strong></p> </td> <td> <p><strong>gut hormone</strong></p> </td> <td> <p><strong>1.408</strong></p> </td> <td> <p><strong>8</strong></p> </td> <td> <p><strong>14</strong></p> </td> </tr> </tbody> </table></figure></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>The R&D Systems Luminex Human Metabolic Panel Quantifies Post-Prandial Hormonal Changes in Human Plasma and Sera</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>To test the new R&D Systems Luminex Metabolic panel, we quantified serum and plasma metabolic biomarkers in 10 people that fasted for 12 hours. One hour after a meal, we quantified post-prandial plasma and serum analyte changes in the same people. As expected, post-prandial (1 hour) levels of C-Peptide and Insulin were elevated in serum and plasma from females (Figure 2) and males (Figure 3). Conversely, Ghrelin levels declined in post-prandial serum and plasma from females and males (Figure 4).</p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/c-peptide-figure-2a-350x210.png" alt="Post fasting concentration of metabolic biomarker c-peptide in female serum and plasma measured by Luminex metabolic panel assay" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p>Figure 2a.  Representative female post-prandial C-Peptide (blue) is increased in SST serum, EDTA plasma and P800 plasma relative to fasting samples (green).</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/insulin-figure-2b-350x210.png" alt="Post fasting concentration of metabolic biomarker insulin in female serum and plasma measured by Luminex metabolic panel assay" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p>Figure 2b.  Representative female post-prandial Insulin (blue) is increased in SST serum, EDTA plasma, and P800 plasma relative to fasting samples (green).</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/c-peptide-figure-3a-350x210.png" alt="Post fasting concentration of metabolic biomarker c-peptide in male serum and plasma measured by Luminex metabolic panel assay" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p>Figure 3a.  Representative male post-prandial C-Peptide (blue) is increased in SST serum, EDTA plasma, and P800 plasma relative to fasting samples (green).</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/insulin-figure-3b-350x210.png" alt="Post fasting concentration of metabolic biomarker insulin in male serum and plasma measured by Luminex metabolic panel assay" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p>Figure 3b.<strong> </strong> Representative male post-prandial Insulin (blue) is increased in SST serum, EDTA plasma, and P800 plasma relative to fasting samples (green).</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/total-ghrelin-figure-4a-350x210.png" alt="Post fasting concentration of metabolic biomarker ghrelin in female serum and plasma measured by Luminex metabolic panel assay" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p>Figure 4a. Post-prandial Ghrelin levels (blue) are decreased in SST serum, EDTA plasma, and P800 plasma relative to fasting levels (green) in representative female serum and plasma.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/total-ghrelin-figure-4b-350x210.png" alt="Post fasting concentration of metabolic biomarker ghrelin in male serum and plasma measured by Luminex metabolic panel assay" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p>Figure 4b. Post-prandial Ghrelin levels (blue) are decreased in SST serum, EDTA plasma, and P800 plasma relative to fasting levels (green) in representative male serum and plasma.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>The R&D Systems Luminex Metabolic Panel Eliminates False Positive Data</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Avoiding false-positive data is key for assay accuracy and specificity. We eliminate false-positive data by adhering to a rigorous quality control process. We also optimize diluents so that analytes on the panel are compatible with each other.</p> <p>Circulating IL-6 levels should be around 1.8 pg/ml<sup>4</sup>. The R&D Systems Metabolic panel IL-6 measurement is close to the expected value, while the competitor assay is artifactually inflated (Table 2). </p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Table 2. Circulating IL-6 data from R&D Systems and competitor Luminex assays. ND=not detectable</p> <figure class="responsive-figure-table"><table class="table table-bordered"> <tbody> <tr> <td> <p><strong>IL-6 </strong></p> </td> <td> <p><strong>R&D Systems (pg/mL)</strong></p> </td> <td> <p><strong>Competitor (pg/mL)</strong></p> </td> </tr> <tr> <td> <p><strong>Serum (1)</strong></p> </td> <td> <p><strong>ND</strong></p> </td> <td> <p><strong>78.4</strong></p> </td> </tr> <tr> <td> <p><strong>Serum (2)</strong></p> </td> <td> <p><strong>1.43</strong></p> </td> <td> <p><strong>765</strong></p> </td> </tr> <tr> <td> <p><strong>Serum (3)</strong></p> </td> <td> <p><strong>ND</strong></p> </td> <td> <p><strong>ND</strong></p> </td> </tr> <tr> <td> <p><strong>Serum (4)</strong></p> </td> <td> <p><strong>4.22</strong></p> </td> <td> <p><strong>450</strong></p> </td> </tr> <tr> <td> <p><strong>Serum (5)</strong></p> </td> <td> <p><strong>4.22</strong></p> </td> <td> <p><strong>ND</strong></p> </td> </tr> <tr> <td> <p><strong>P800 EDTA (1)</strong></p> </td> <td> <p><strong>ND</strong></p> </td> <td> <p><strong>ND</strong></p> </td> </tr> <tr> <td> <p><strong>P800 EDTA (2)</strong></p> </td> <td> <p><strong>2.60</strong></p> </td> <td> <p><strong>1621</strong></p> </td> </tr> <tr> <td> <p><strong>P800 EDTA (3)</strong></p> </td> <td> <p><strong>4.74</strong></p> </td> <td> <p><strong>493</strong></p> </td> </tr> </tbody> </table></figure></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p> </p> <p> </p> <p>Linearity of dilution experiments are an excellent way to experimentally assess false positive data. In Figure 5 we see that the competition’s insulin Luminex assay fails to maintain linearity of dilution at any of the dilutions tested. Conversely, the R&D Systems insulin assay is consistently within the acceptable range of 80-120% recovery.</p> <p> </p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/insulin-natural-linearity-525x425.jpg" alt="Linearity of dilution data showing R&D Systems Luminex metabolic panel versus competitor percent recovery for insulin measurement" loading="lazy" > </div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_image_caption"> <div>Figure 5. The competitor assay fails to maintain linearity.</div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p> </p> <p> </p> <p> </p> <p>Five serum and three P800 EDTA samples were tested using R&D Systems and competitor metabolic Luminex panels. Fasting insulin levels should be less than 1000 pg/ml (red line)<sup>5</sup>. Fasting insulin levels are artifactually high in competitor assays but not R&D Systems Luminex Assays.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/fasting-insulin-525x425.jpg" alt="Fasting insulin concentrations measured by R&D Systems Luminex assay versus competitor" loading="lazy" > </div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_image_caption"> <div>Figure 6. The competitor assay data is artifactually high.</div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Methods: Sample Collection and Preparation</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Serum Separating tube (SST) serum and EDTA plasma samples were collected from 10 donors for all experiments.  The initial blood draw was collected after 12 hours of fasting.  The donors were then fed a mixed meal and told to return 1 hour after finishing the meal for a second blood draw.  SST samples were injected with Dipeptidyl Peptidase-<em>4</em> (DPP4) inhibitors immediately after collection then allowed to sit at room temp to clot prior to centrifugation.  Normal EDTA samples were injected with DPP4 inhibitors immediately after collection then placed on ice until centrifugation.  Both the SST serum and normal EDTA samples were separated from the blood cells and Protease Inhibitor Cocktail I (<a href="https://www.tocris.com/products/protease-inhibitor-cocktail-i_5500">Tocris Catalog number 5500</a>) was added to a 1X concentration.  P800 EDTA samples (BD Biosciences catalog number 366420) were drawn and placed on ice.  No additional inhibitors were added to these tubes, as they contain a proprietary protease inhibitor cocktail. </p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Conclusions and References</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Here we have demonstrated that the R&D Systems Luminex Human Metabolic Panel can be used to quantify metabolic biomarkers in human plasma and serum. This assay allows for the simultaneous quantification of 12 metabolic biomarkers in each 50 ul sample.  Our Luminex assay is both faster and provides superior performance to competitor Luminex assays.</p> <p> </p> <ol> <li>Rochlani, Y et al (2017) Metabolic syndrome: pathophysiology management, and modulation by natural compounds. <em>Ther Adv Cardiovasc Dis </em><strong>11</strong> 215 PMID: 28639538</li> <li>Edwardson CL et al (2012) Association of Sedentary Behaviour With Metabolic Syndrome: A Meta-Analysis. <em>Plos One </em><strong>7 </strong>PMID: 22514690</li> <li>Hirode G and Wong RJ (2020) Trends in the Prevalence of Metabolic Syndrome in the United States 2011-2016. <em>JAMA</em> <strong>323 </strong>24 PMID: 32573660</li> <li><a href="https://www.mayocliniclabs.com/test-catalog/Clinical+and+Interpretive/63020">https://www.mayocliniclabs.com/test-catalog/Clinical+and+Interpretive/63020</a></li> <li><a href="https://www.mayocliniclabs.com/test-catalog/Clinical+and+Interpretive/62990">https://www.mayocliniclabs.com/test-catalog/Clinical+and+Interpretive/6…</a></li> </ol> <p> </p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <a href="/p/luminex-assays/human-metabolic-luminex-performance-premixed-kit_fcstm19" class="btn btn-primary" type="link"> Purchase the Luminex Metabolic Panel </a> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Metabolic Research Resources</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/metabolic-syndrome-signaling" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/images/Research_areas/ps_metabolic-syndrome-650x433.jpg" alt="Metabolic Syndrome poster" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/metabolic-syndrome-signaling" > <h3 class="card_title_htag"> <div><h3>Metabolic Syndrome Poster</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Download or request the poster to learn more about metabolic syndrome signaling. 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