Next-generation sequencing tool shows 96% specificity in thyroid cancer detection

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"> 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</li> </ul> </div> <script type="text/javascript"> if (typeof (savedoc) != "function") { function savedoc() { mustlogin(); } } </script>  <div class="wyContent"> <p>SAN JUAN, Puerto Rico — A next-generation sequencing tool allows for improved accuracy in diagnosis of thyroid nodules and treatment of thyroid cancer, according to a presenter here.</p> <p>“The ThyroSeq next-generation sequencing approach allows simultaneous testing for multiple mutations with high accuracy and sensitivity. It requires a minimal amount of nucleic acids and can be performed on [fine needle aspiration] and tissue samples,” <strong>Marina Nikiforova, MD, FCAP, </strong>director of the University of Pittsburgh Medical Center Molecular Oncology and Personalized Diagnostics laboratory, said. “ThyroSeq can be used for high-throughput genotyping of thyroid samples, helping to improve accuracy of diagnosis of thyroid nodules and prognostication of thyroid cancer.”</p> <div class="mug right"><img width="90" height="120" alt="Marina Nikiforova, MD, FCAP" src="https://web.archive.org/web/20160909163926im_/http://m3.wyanokecdn.com/8028fd7120a28edac6f5bca6ca3776ad.jpg">  <p class="name"><strong>Marina Nikiforova</strong></p> </div> <p></p> <p>This tool was launched at the University of Pittsburgh on Oct. 1, 2013, and Nikiforova and colleagues published some data in the <i>Journal of Clinical Endocrinology & Metabolism</i> this year. These results indicated that ThyroSeq’s accuracy for mutation detection was 100% with a sensitivity of 3% to 5% of mutant alleles or fusion transcripts. They also showed that the next-generation tool detected mutations in a significant number of carcinomas, including in more than 70% of papillary, anaplastic and medullary thyroid cancers, whereas mutations were detected in only 6% of benign nodules. The most common mutations were <i>BRAF</i> and <em>RAS</em> and a number of additional, more rare mutations was found in thyroid cancer samples.</p> <p>In this presentation, Nikiforova expanded her previous observation and discussed an examination of 61 FNA samples with the most common type of indeterminate cytology, ie, follicular neoplasm, using both ThyroSeq and surgical excision. ThyroSeq and surgical pathology agreed on all but four specimens. Of those four, two were found malignant by ThyroSeq but benign by surgical biopsy, and two were found benign by ThyroSeq but malignant by surgical biopsy.</p> <p>“Only two tumors were missed, for 96% specificity and 93% of overall accuracy,” Nikiforova said. </p> <p>Overall, she added, Thyroseq is able to detect mutations in 12 genes and 45 gene fusions and only required 10 ng of DNA and RNA, so that more than 98% of all thyroid FNA samples worked. Importantly, a number of mutational markers added to the ThyroSeq panel can allow to identify those tumors that are likely to be more aggressive and therefore need to be treated more extensively. </p> <p>“This will bring management of thyroid cancer to the next level,” she said. – <i>by Katrina Altersitz</i></p> <p><strong>For more information: </strong>Nikiforova MN. Oral Abstract 13. Presented at: the 83rd Annual Meeting of the American Thyroid Association; Oct. 16-20, 2013; San Juan, Puerto Rico.</p> <p><strong>Disclosure: </strong>Nikiforova reports no relevant financial disclosures.</p> </div>  <div class="fig left"> <p class="figcaption"> </p> </div> <div class="wyPagination">   </div>    <h4><a href="/web/20160909163926/http://www.healio.com/endocrinology/resource-centers/{4a64d6e7-7edd-4155-a163-741f23ec41a0}/ata-annual-meeting-2013">See more from ATA Annual Meeting 2013</a></h4>      <ul class="module user-tools desktop-view" data-module="User Tools and Social" data-isroot="1"> <li id="main_0_maintwocolumnleft_0_ctl00_SocialBottom_liComments" class="tools"><a href="/web/20160909163926/http://www.healio.com/endocrinology/thyroid/news/online/%7Baeda2b85-4e75-4a80-9b81-85c30decfb0b%7D/next-generation-sequencing-tool-demonstrates-high-accuracy-in-diagnosis-of-thyroid-cancer/comments"><i class="fa fa-comment"></i> <span class="tool-text alt"> READ/SUBMIT 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class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <a class="close togclose" data-dismiss="modal"><span aria-hidden="true">×</span><span class="sr-only">Close</span></a> <h4 class="modal-title">Please Register</h4> </div> <div class="modal-body"> <div id="instructions" class="form-group" style="font-weight: bold;">In order to view this content, you must register below or <a href="#" onclick="showLoginForm();">log in</a>.</div> <div id="outputmessage" class="alert alert-danger" style="display: none;"></div> <div id="identifyform"> <div class="form-group"> <label class="col-md-3 control-label">First Name</label> <div class="col-md-9"> <input type="text" class="form-control" name="firstname" id="firstname"> </div> </div> <div class="form-group"> <label class="col-md-3 control-label">Last Name</label> <div class="col-md-9"> <input type="text" class="form-control" name="lastname" id="lastname"> </div> </div> <div class="form-group"> <label class="col-md-3 control-label">Email</label> <div class="col-md-9"> <input type="text" class="form-control" name="email" id="email"> </div> </div> <div class="form-group"> <div class="col-md-5 col-md-offset-3"> <input type="submit" class="btn btn-warning" id="dripcheck" value="Submit"> <img class="loading" style="display: none;" src="https://web.archive.org/web/20160909163926im_/http://i3.wyanokecdn.com/ajax-loader.gif" alt="Loading..."> </div> </div> </div> <div id="loginform" style="display: none;"> <div class="form-group"> <label class="col-md-3 control-label">Email</label> <div class="col-md-9"> <input type="text" class="form-control" name="username" id="username"> </div> </div> <div class="form-group"> <label class="col-md-3 control-label">Password</label> <div class="col-md-9"> <input type="password" class="form-control" name="password" id="password"> </div> </div> <div class="form-group"> <div class="col-md-9 col-md-offset-3"> <input type="submit" class="btn btn-warning" id="dologin" value="Login" style="float: none; left:0; top:0; position: relative;"> <img class="loading" style="display: none;" src="https://web.archive.org/web/20160909163926im_/http://i3.wyanokecdn.com/ajax-loader.gif" alt="Loading..."> <a href="#" onclick=" showForgotPasswordForm() ">Forgot Password?</a> </div> </div> </div> <div id="forgotpassword" style="display: none;"> <div class="form-group"> <label class="col-md-3 control-label">Email</label> <div class="col-md-9"> <input type="text" class="form-control" name="emailaddress" id="emailaddress"> </div> </div> <div class="form-group"> <div class="col-md-5 col-md-offset-3"> <button type="submit" class="btn btn-warning" id="sendpassword">Send</button> <img class="loading" style="display: none;" src="https://web.archive.org/web/20160909163926im_/http://i3.wyanokecdn.com/ajax-loader.gif" alt="Loading..."> </div> </div> <div class="form-group" style="font-weight: bold;"> In order to view this content, you must register below or <a href="#" onclick="showLoginForm();">log in</a>. </div> </div> </div> <div class="modal-footer togclose"> <a class="btn" data-dismiss="modal"><i class="fa fa-times"></i> Close</a> </div> </div>  </div>  </div>  <style>.form-group { overflow: hidden; }</style> <script>$j(document).ready(function() { $j('form').submit(function(ev) { if ($j('#dripmodal').is(':visible')) { ev.preventDefault(); if ($j('#loginform').is(':visible')) { doLogin(); return; } if ($j('#identifyform').is(':visible')) { checkDripReg(); return; } if ($j('#forgotpassword').is(':visible')) { forgotPassword(); return; } } }); $j('#dripmodal').on('hide.bs.modal', function() { $j("#identifyform").show(); $j("#loginform").hide(); $j("#forgotpassword").hide(); $j("#outputmessage").html(""); $j("#outputmessage").hide(); $j("#instructions").show(); $j("#instructions").html("In order to view this content, you must register below or <a href='#' onclick='showLoginForm();'>log in</a>."); $j('#dripmodal .modal-title').html("Please Register"); }); //rules $j('#identifyform') .bootstrapValidator({ feedbackIcons: { valid: null, invalid: null, validating: null }, fields: { firstname: { validators: { notEmpty: { message: 'Your first name is required' } } }, lastname: { validators: { notEmpty: { message: 'Your last name is required' } } }, email: { validators: { notEmpty: { message: 'Your email address is required' }, emailAddress: { message: 'Your email address is in an invalid format' } } } } }).on('success.field.bv', function(e, data) { var $parent = data.element.parents('.form-group'); $parent.removeClass('has-success'); $parent.find('.form-control-feedback[data-bv-icon-for="' + data.field + '"]').hide(); });; $j("#dripcheck").click(function(e) { e.preventDefault(); checkDripReg(); }); $j("#dologin").click(function(e) { e.preventDefault(); doLogin(); }); $j("#sendpassword").click(function(e) { e.preventDefault(); forgotPassword(); }); function checkDripReg() { var email = $j('#email').val(); var firstname = $j('#firstname').val(); var lastname = $j('#lastname').val(); if (!email || !firstname || !lastname) { $j("#outputmessage").show(); $j("#outputmessage").html("Please fill out all fields before continuing.<br/>"); return; } var jData = { "email": email, "fname": firstname, "lname": lastname, "source" : "Drip Registration" }; $j(".loading").show(); $j.ajax({ type: "POST", url: "/registrationapp/json/checkemailwithshortregacc", data: JSON.stringify(jData), contentType: "application/json; charset=utf-8", success: function(msg) { var response = JSON.stringify(msg); var obj = $j.parseJSON(response); //$j("#outputmessage").text(response); if (obj.CheckEmailWithShortRegAccResult.AccountPreviouslyExist == true && obj.CheckEmailWithShortRegAccResult.EmailExist == false) { if (window.ga) { ga('send', 'event', 'Drip Registration', 'X Click Registration', 'Prompt Login', 0, { 'nonInteraction': true }); } $j("#identifyform").hide(); $j('#dripmodal .modal-title').html("Please Login"); $j("#username").val(email); $j("#instructions").html('You already have an account with Healio.com. Please log in.'); $j("#instructions").show(); $j("#outputmessage").hide(); $j("#outputmessage").html(''); $j('#loginform').show(); $j('.loading').hide(); return; } else { //invalid email domain if (obj.CheckEmailWithShortRegAccResult.InvalidEmail == true) { $j("#outputmessage").show(); $j("#outputmessage").html('' + obj.CheckEmailWithShortRegAccResult.Message + ''); $j('#email').focus(); $j('.loading').hide(); return; } if (clickedLink != null && clickedLink != '') { // setting the cookie for those who came through the drip registration so they will able to step through all but post test step of activity (gamification) setCookie("edlabcmedripreg", obj.CheckEmailWithShortRegAccResult.UserId, 1); //$j('#dripmodal').modal('hide'); $j("#outputmessage").hide(); $j("#outputmessage").html(''); window.location.assign(clickedLink); return; } if (window.ga) { ga('send', 'event', 'Drip Registration', 'X Click Registration', 'Completed Short Registration', 1, { 'nonInteraction': true }); } var location = "/footer/account-information/create-new-user" + obj.CheckEmailWithShortRegAccResult.Message + "&returnurl=" + window.location.pathname; window.location.assign(location); return; } }, error: function(jqXHR, textStatus, errorThrown) { //alert(textStatus + ": please make sure you fill in all fields."); $j('.loading').hide(); }, complete: function() { //$j("#outputmessage").html(""); //$j('.loading').hide(); } }); } function doLogin() { var username = $j("#username").val(); var password = $j("#password").val(); if (!username || !password) { $j("#outputmessage").show(); $j("#outputmessage").html("Please fill out all fields before continuing.<br/>"); return; } $j(".loading").show(); var jData = {}; jData.email = username; jData.password = password; $j.ajax({ type: "POST", url: "/registrationapp/json/login", data: JSON.stringify(jData), contentType: "application/json; charset=utf-8", dataType: "json", success: function(msg) { var response = JSON.stringify(msg); var obj = $j.parseJSON(response); if (obj.LoginResult.Message == "Login Successful") { if (window.ga) { ga('send', 'event', 'Drip Registration', 'X Click Registration', 'Successful Login', 1, { 'nonInteraction': true }); } //$j("#outputmessage").html("<p>Welcome back. You may continue</p>"); // "Gourmand" cookies need to be set setCookie("edlabcmedriplogin", obj.LoginResult.UserId, ""); if (clickedLink != '') { window.location.assign(clickedLink); return; } else { location.reload(true); return; } //$j('#dripmodal').modal('hide'); } else { $j("#outputmessage").show(); $j("#outputmessage").html(obj.LoginResult.Message); $j('.loading').hide(); } }, error: function(jqXHR, textStatus, errorThrown) { alert(textStatus + ": please make sure you fill in all fields."); $j('.loading').hide(); }, complete: function() { //$j('.loading').hide(); } }); }; function forgotPassword() { var emailaddress = $j("#emailaddress").val(); if (!emailaddress) { $j("#outputmessage").show(); $j("#outputmessage").html("Please fill out all fields before continuing.<br/>"); return; } $j(".loading").show(); var jData = {}; jData.email = emailaddress; jData.website = "Healio"; jData.itemId = "{9FFB2568-23D5-47DA-85C9-C55E1EC8F4FF}"; jData.returnurl = window.location.pathname; $j.ajax({ type: "POST", url: "/registrationapp/json/forgetpassword", data: JSON.stringify(jData), contentType: "application/json; charset=utf-8", dataType: "json", success: function(msg) { var response = JSON.stringify(msg); var obj = $j.parseJSON(response); if (obj.ForgetPasswordResult.EmailSent) { $j("#outputmessage").show(); $j("#instructions").hide(); $j("#outputmessage").html("An email has been sent to the email address provided. Please follow the instructions on how to reset your password."); $j("#forgotpassword").hide(); $j("#loginform").show(); $j("#password").val(""); $j('#dripmodal .modal-title').html("Please Login"); $j("#username").val(emailaddress); } else { $j("#outputmessage").show(); $j("#outputmessage").html(obj.ForgetPasswordResult.Message); } }, error: function(jqXHR, textStatus, errorThrown) { //alert(textStatus + ": please make sure you fill in all fields."); }, complete: function() { $j('.loading').hide(); } }); } }); function showLoginForm() { $j("#identifyform").hide(); $j('#dripmodal .modal-title').html("Please Login"); $j("#outputmessage").html(""); $j('#forgotpassword').hide(); //$j("#instructions").hide(); $j("#instructions").html("You already have an account with Healio.com. 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