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class='breadcrumb-item-label' title='Technologies'>Technologies</span></a></li><li class='breadcrumb-item breadcrumb-item-current'><span class='breadcrumb-item-label' title='Tools, Methods & Assays'>Tools, Methods & Assays</span></li></ol> <script type='application/ld+json'>{"@context":"https:\/\/schema.org","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Technologies","item":"https:\/\/embl-em.de\/technologies\/"},{"@type":"ListItem","position":2,"name":"Tools, Methods & Assays"}]}</script> </div> </div> </div> <article id="page-1735" class="page-single page-1735 page-tools-methods-assays"> <section class="content-section tbe-custom-background-color tbe-custom-background-color-white"> <div class="grid"> <div class="row"> <div class="col desktop-8 tablet-8 phablet-12 phone-12 tbe-module-marginal-column-right"> <p class="overline-h1">Technologies</p> <h1 id='tools-methods-assays'>Tools, Methods & Assays</h1> <p class="lead-paragraph">As Europe’s flagship laboratory for molecular biology, EMBL-inventions are scattered across the whole range of molecular biology research.</p> <p>The technologies cover areas including protein expression tools, nanotechnology, research tools & platforms, kit-ready assays, diagnostic tools and targets that can be further developed into future drugs.</p> <div id="accordion-1656487843624" class="accordion" data-open="1"> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item" open=""> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title">SComatic</span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p><span class="accordion-head-title">Detection of somatic mutations in high-throughput single-cell profiling data sets</span><br /> <a class="button" href="https://embl-em.de/wp-content/uploads/2024/04/TechnologyOffer_SComatic-1.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item" data-mce-open="open"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong><b>EMBL-EBI Agri-Tech Partnership</b> </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Transforming Agri-Tech R&D with actionable insights<br /> <a class="button" href="https://embl-em.de/wp-content/uploads/2023/12/EMBLEM_2024-0026_EMBL-EBI-Agri-Tech-Partnership_Tech-Offer.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item" data-mce-open="open"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong><b>Decoding the Cancer Genome: A Patient-Centric Journey from Telomere to Telomere</b> </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Uncovering the complexity of genomic rearrangement bridging genetic insights with patient-centric approaches for enhanced diagnostic and prognostic.<br /> <a class="button" href="https://embl-em.de/wp-content/uploads/2024/01/EMBLEM_2024-009_-Decoding-the-Cancer-Genome_EMBL-HD_Korbel_Tech-Offer.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong><b>Decrypting Patient-Specific Cell Signaling: Network-Based Strategies for Personalized Insights</b> </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Decoding the rules governing context-specific human cell signalling responses through data-driven network-based strategies, ensuring optimised and tailored solutions for patients.<br /> <a class="button" href="https://embl-em.de/wp-content/uploads/2023/10/2024-013_Evangelia_TechnologyOffer.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong><b>Enhanced Data Interoperability: Leveraging the Ontology Lookup Service (OLS), and the Ontology Xref Service (OxO)</b> </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>A modular ontology-based toolkit empowering seamless cross-domain semantic enrichment, data management and analysis.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/10/2024-014-Helen-parkinson_TechnologyOffer.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong><b>Empowering Agriculture, Aquaculture, Biodiversity Conservation and Genetic Innovation with Advanced Data Processing</b> </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Fostering genomic insights and biodiversity for a sustainable future through efficient data management, visualization, presentation, and seamless coordination and standardization.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/09/Peter-Harrison_TechnologyOffer_2024-007.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong><b>New Approaches for Vaccination: Modular pathogens analysis with safe, seamless, and user-friendly epidemiology methods</b> </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner">Enabling proactive pathogen management and informed interventions against infectious diseases with genomic epidemiology methods.<br /> <a class="button" href="https://embl-em.de/wp-content/uploads/2023/09/2024-006_John-Lees_TechnologyOffer.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong><b>Morphological Quantification of Bioimages for Advanced Phenotyping</b> </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Investigate living systems across scales with customized image quantification methods.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/07/2024-002_Virginie-Uhlmann_TechnologyOffer_20230707.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>QSPeed: <b>Validation of Quantitative Systems Pharmacology (QSP) Models</b> </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Streamlining QSP Modelling with Curated Building-Blocks and Tailored Support</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/07/2024-001-TechnologyOffer_Validation-of-QSP-Models_07-2023.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Photo-Micropatterning – Creating functionalized cryoEM grids for studying cell architecture</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>A method for creating funcionalized cryoEM grids that allow to control cell shape and study cell architecture</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/01/TechOffer_2019-037.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487843624" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>EasyGrid – A device for automated preparation and quality control of CyroEM samples </strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>A prototype device that fully automates the procedure using in-line plasma treatment, picolitre drop dispensers, jet vitrification and automated cryo-storage.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/01/TechOffer_2018-032.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>A comprehensive modular epigenome editing platform</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Comprehensive modular epigenome editing platform for precise programming of specific chromatin modifications at target loci. It can serve as powerful precision therapeutics tool to restore appropriate epigenetic marks and gene activity for the amelioration of various diseases.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/03/TechnologyOffer_EpiEditing_01-2023.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Novel drug combinations against multi-resistant bacteria</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>The technology comprises novel drug combinations against multi-resistant bacteria and a platform to discover further combinations of antibiotics and non-antibiotic drugs that act synergetically against multi-resistant bacteria.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/03/TechnologyOffer_Antimicrobial-Combinations_01-2023.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>“Sleeping Beauty” – A novel Sleeping Beauty transposase system for development of gene therapies</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>A novel Sleeping Beauty transposase system for the development of gene therapies, based on improved delivery of the recombinant transposase protein with several advantages such as increased safety, efficacy, stability, dose-dependency etc.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/04/TechnologyOffer_SleepingBeauty_04-2023.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Novel autotaxin inhibitors</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner">The technology comprises a novel set of autotaxin inhibitors with several advantages over the state of the art, e.g. derivation from natural sources, increased non-competitive inhibitory activity, different conceivable application scenarios and administration routes.<br /> One compound was developed further and characterized comprehensively.<br /> Autotaxin inhibitors might be applied for the treatment of various diseases such as inflammatory diseases, neurodegenerative diseases, multiple sclerosis, atherosclerosis, cancer etc.<a class="button" href="https://embl-em.de/wp-content/uploads/2023/03/TechnologyOffer_Autotaxins_01-2023.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>“SPION-CCPMs” – Iron nanoparticles as adjuvant lung cancer therapeutic</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Novel iron-loaded nanoparticles (super-paramagnetic iron oxide nanoparticles-loaded core cross-linked polymeric micelles; SPION-CCPMs) improve iron delivery and trigger increased inflammatory responses, which is beneficiary for the treatment of several diseases. In lung cancer models (in vitro and in vivo), SPION-CCPMs induced an anti-tumor phenotype in macrophages, resulting in oxidative stress and the destruction of cancer cells.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/03/TechnologyOffer_SPIONS_01-2023.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>“Spacial omics” – 3D cell positioning by optical labelling</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>The technology enables tracing back the original position of a cell in a tissue by fluorescent labelling. It represents a central research tool and helps to reduce time and errors.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/03/TechnologyOffer_Spacial-Omics_03-2023.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Pan-cancer biomarkers for early tumor detection via liquid biopsy</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>A new type of telomere fusion was found that is not present in the blood of healthy donors but well detectable in the blood of cancer patients. The technology comprises computational tools for the reliable identification of these fusions. Additionally, predictive models have been established that allow for the detection of the presence of a tumor using sequencing data from a blood sample.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2024/05/TechnologyOffer_Pancancer-1.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>“scTRIP” – Single Cell Tri-Channel Processing</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>A technology for the systematic, detailed and accurate detection of structural DNA variations (e.g. deletions, duplications etc.), which includes all known forms of karyotypic abnormality in single cells. The technology can help to better understand disease, enable precision medicine approaches, and allows for quality control in gene and cell therapy approaches.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/03/TechnologyOffer_02-2023_JD.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Method for ENO1-based compound screening to modulate glycolysis in cancer cells</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>The overexpression of ENO1 has been associated with multiple tumors and has therefore become an interesting target for the development of new compounds. We present a technology to screen for compounds that enable the modulation of glycolysis in cancer cells.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/03/TechnologyOffer_ENO1_02-2023.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Open Targets</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Open Targets is an innovative, large-scale, multi-year public-private partnership that uses human genetics, genomics and other ‘omics data to systematically identify and prioritize the best targets to safely and effectively treat common and rare diseases.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/03/Open_Targets_TechnologyOffer_02-2023_JD_3-1.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487844916" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Enzymes for Green and Clean Synthesis and Bioremediation</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Platforms for high-throughput virtual screening for novel enzyme reactions for synthesis and enzyme improvement</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2023/01/TechOffer_2021-020.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487846776" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>“ESPRIT” – A systematic approach for generating soluble protein variants</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Proteins often express insolubly which severely limits their usefulness in areas such as structural analysis by crystallography and NMR. Several systems have been described that aim to identify soluble protein variants generated by random mutagenesis or truncation. These methods usually involve fusion of a C-terminal “solubility reporter” (e.g. GFP, CAT or beta galactosidase). The tag used in the methods described above are large and thus enhance the solubility profile of the fusion product. The solubility of the thus created fusion protein is highly dependent on the solubility phenotype of the tag used. By using a smaller tag the solubility influence of the tag is reduced leading to a reduction of false positives.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2016/05/EMBLEM_ESPRIT.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487848124" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Cell-based method for the analysis of protein-protein interactions</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Protein-protein interactions are crucial for virtually all cellular processes. Therefore analysis of such interactions is becoming increasingly important in molecular biology, biochemistry and computational biology. Furthermore, disturbed protein-protein interactions can contribute to diseases rendering the identification of protein-protein interaction inhibitors highly desirable in drug discovery. Traditional technologies used for analysis of protein-protein interactions share the major drawback that the experimental set-up is highly artificial, questioning the physiological relevance of such interactions in vivo. The technology presented here allows for reliable analysis of intracellular protein-protein interactions based on a translocation principle.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2016/05/EMBLEM_PP_Interactions.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487849179" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Novel biomarkers for the detection of testicular carcinoma <em>in situ</em> and derived cancers in human samples</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>Virtually all testicular cancers originate from the same precursor, the carcinoma in situ (CIS) cell. If left untreated, CIS will invariably progress into testicular cancer. Unfortunately, the disease is rarely diagnosed at this asymptomatic stage, since it hitherto has required a testicular biopsy to identify CIS. The aim of the current work is to develop and validate a non-invasive diagnostic test for early detection of testicular cancer based on identification of pre-invasive CIS cells in semen samples.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2016/05/EMBLEM_Cancer_Biomarkers.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> <details id="accordion-1656487843624-item-1656487850188" class="accordion-item"> <summary class="accordion-head"><span class="accordion-head-icon"> </span><span class="accordion-head-title"><strong>Phasing macromolecular structures by UV-induced damage</strong></span></summary> <div class="accordion-body"> <div class="accordion-body-inner"> <p>This invention relates to a novel method to phase macromolecular crystal structures using damage induced by UV radiation (UV-RIP). Compared to existing techniques this method shows the advantage that it can be performed on a single crystal of the native protein and introduces only specific changes. The technology can be used independently of a synchrotron.</p> <p><a class="button" href="https://embl-em.de/wp-content/uploads/2016/05/EMBLEM_UV_Structure_Determination.pdf" target="_blank" rel="noopener noreferrer"><span class="has-icon" data-icon="pdf">Download PDF</span></a></p> </div> </div> </details> </div> </div> <div class="col desktop-4 tablet-4 phablet-12 phone-12"> <aside id='sidebar-page' class='the-sidebar page-sidebar'><div id="recent_posts_1990kb-4" class="widget widget_recent_entries"><h6 class="widget-title">Latest News</h6> <ul class="recent-posts-list"> <li id="recent-posts-item-3073" class="recent-posts-item"> <div class="recent-posts-item-thumbnail-column margin-fix"> <a class="post-overview-item-image" href="https://embl-em.de/latest-news/2024/10/25/evaluating-the-link-between-chemicals-and-declining-insect-populations-003073/" style="background-image: url('https://embl-em.de/wp-content/uploads/2024/10/Crocker_agrochemicals_final-wp.jpg');"> <span 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