CD8 Antibody (76-2-11) [DyLight 405] (NBP1-28237V) by Novus, Part of Bio-Techne

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data-time_datapoints="-0.11343789100647" data-time_template="0.11343789100647" data-time_post_epcc="0.77523803710938" data-time_epcc="-0.11343789100647" data-render_time="Friday 4th of October 2024 10:56:29 AM" data-fallback="FALSE" data-time_to_process_data="0.090274095535278" class="px-4 container" class="container"> <div id="above-fold-section"> <div class="mb-2 o__product-summary"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> CD8 Antibody (76-2-11) [DyLight 405] </h1> <h2 class="font-weight-normal h4"> Novus Biologicals, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">NBP1-28237V</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="submit_review_wrapper" class="col-auto d-flex align-self-center"> <div id="submit_review"> <a href="/reviews/NBP1-28237V" 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data-section="component-product-image-tree" class="vertical lightbox" data-mdb-zoom-effect="true"> <div id="product-image-gallery" class="row"> <div class="col-md-11 border rounded d-flex align-items-center justify-content-center product-image-lightbox"> <div class="text-center my-2" data-brand="novus"> <img src="https://resources.rndsystems.com/categoryimages/novus_antibodies.jpg" alt="Novus Antibodies" title="Novus Antibodies" data-caption="" data-badges='' data-id="0" width="250" height="250" fetchpriority="high" as="image" class="search_result_thumbnail_image active" /> </div> </div> </div> </div> </div> <div class="col-xl-6 mt-2" id="orderdetails-wrapper" data-section="order_detail_block"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="NBP1-28237V"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-3 p-0 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-2 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="NBP1-28237V" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">NBP1-28237V</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <div class="atc-control ProductCart-control text-right"> <div class="row container d-flex justify-content-end mx-0 mb-3 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="" class="atc-print-quote-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100">Print Quote</a> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=NBP1-28237V" class="atc-bulk-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> <div class="atc-container order_detail_wrapper conjugates-and-formulations-wrapper my-2 position-relative"> <div class="button-group text-right pl-2"> <a href="#conjugates-NBP1-28237V" class="btn btn-secondary atc-view-pricing atc-view-pricing-products collapsed text-nowrap mb-2" data-id="conjugates-NBP1-28237V" data-toggle="collapse" aria-expanded="false" aria-controls="conjugates">View Available Conjugates </a> <a href="#formulations-NBP1-28237V" class="btn btn-secondary atc-view-pricing atc-view-pricing-products collapsed text-nowrap mb-2" data-id="formulations-NBP1-28237V" data-toggle="collapse" aria-expanded="false" aria-controls="formulations">View Available Formulations </a> </div> <div id="conjugates-NBP1-28237V" class="conjugates-and-formulations-container collapse position-absolute w-100"> <div class="atc-container border rounded bg-white shadow p-2 mt-1"> <div class="commerce-atc-datasheet atc-block-collapsible" data-type="conjugates_and_formulations" data-blockid="nbp1-28237v-conjugate-atc-block"> <div id="nbp1-28237v-conjugate-atc-block" class="atc-lines"> <div class="atc-header row pb-1 m-0"> <div class="atc-head col-4 p-0 font-weight-bold d-sm-none d-md-block">Conjugate</div> <div class="atc-head col-4 p-0 font-weight-bold">Catalog #</div> <div class="atc-head col-4"></div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237AF405" data-titleoverwrite="Alexa Fluor 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af405">Alexa Fluor 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af405">NBP1-28237AF405</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af405" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237AF488" data-titleoverwrite="Alexa Fluor 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af488">Alexa Fluor 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af488">NBP1-28237AF488</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af488" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237AF647" data-titleoverwrite="Alexa Fluor 647"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af647">Alexa Fluor 647</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af647">NBP1-28237AF647</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af647" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237AF700" data-titleoverwrite="Alexa Fluor 700"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af700">Alexa Fluor 700</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af700">NBP1-28237AF700</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237af700" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237APC" data-titleoverwrite="Allophycocyanin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237apc">Allophycocyanin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237apc">NBP1-28237APC</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237apc" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237UV" data-titleoverwrite="DyLight 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237uv">DyLight 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237uv">NBP1-28237UV</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237uv" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237G" data-titleoverwrite="DyLight 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237g">DyLight 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237g">NBP1-28237G</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237g" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237FR" data-titleoverwrite="DyLight 680"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237fr">DyLight 680</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237fr">NBP1-28237FR</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237fr" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237IR" data-titleoverwrite="DyLight 755"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237ir">DyLight 755</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237ir">NBP1-28237IR</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237ir" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28238" data-titleoverwrite="FITC"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28238">FITC</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28238">NBP1-28238</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28238" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237PE" data-titleoverwrite="PE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237pe">PE</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237pe">NBP1-28237PE</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237pe" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237PCP" data-titleoverwrite="PerCP"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237pcp">PerCP</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237pcp">NBP1-28237PCP</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237pcp" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> </div> </div> </div> </div> <div id="formulations-NBP1-28237V" class="conjugates-and-formulations-container collapse position-absolute w-100"> <div class="atc-container border rounded bg-white shadow p-2 mt-1"> <div class="commerce-atc-datasheet atc-block-collapsible" data-type="conjugates_and_formulations" data-blockid="nbp1-28237v-formulations-atc-block"> <div id="nbp1-28237v-formulations-atc-block" class="atc-lines"> <div class="atc-header row pb-1 m-0"> <div class="atc-head col-4 p-0 font-weight-bold d-sm-none d-md-block">Forumulation</div> <div class="atc-head col-4 p-0 font-weight-bold">Catalog #</div> <div class="atc-head col-4"></div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NBP1-28237" data-titleoverwrite="Azide and BSA Free"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237">Azide and BSA Free</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237">NBP1-28237</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/cd8-antibody-76-2-11_nbp1-28237" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> </div> </div> </div> </div> </div> <div id="guarantee-statement" class="text-right" data-section="section-base--guarantee-statement"> <span class="icon-icon-check-circle"></span> <a href="/guarantee">100% Guarantee</a> </div> </div> </div> </div> </div> <div id="tabs-distributor" class="row"> <div class="tab-content col-xl-9 mt-2"> <div data-section="section-base--nav-tabs-content"> <ul class="nav nav-tabs nav-fill" id="productTab" role="tablist"> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor active" href="#tab-technical_data" id="technical_data-tab" data-toggle="tab" data-target="#technical_data" type="button" role="tab" aria-controls="technical_data" aria-selected="true"> <p class="m-0">Technical Data</p> </a> </li> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor " href="#tab-citations_reviews" id="citations_reviews-tab" data-toggle="tab" data-target="#citations_reviews" type="button" role="tab" aria-controls="citations_reviews" aria-selected="false"> <p class="m-0">Citations & Reviews</p> </a> </li> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor " href="#tab-protocols_faqs" id="protocols_faqs-tab" data-toggle="tab" data-target="#protocols_faqs" type="button" 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data-section="section-base--technical-anchors"> <ul class="product-pages-anchors nav mt-2"> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor active" href="#product-summary"> Product Summary</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#applications"> Applications</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#preparation--storage"> Preparation & Storage</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#background"> Background</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#product-documents"> Product Documents</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#specific-notices"> Specific Notices</a> </li> </ul> </div> <div id="key_details" class="py-5 bt-pp-border-bottom" data-section="section-base--key-details"> <h3>Key Product Details</h3> <div class="mt-5 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Species Reactivity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <h4 class="d-block">Validated:</h4> <div class="d-block pb-2"> Porcine </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Applications</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <div class="d-block pb-2"> Flow Cytometry </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Label</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> DyLight 405 (Excitation = 400 nm, Emission = 420 nm) </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Antibody Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal Mouse IgG2a Kappa Clone # 76-2-11 </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Concentration</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Please see the vial label for concentration. If unlisted please contact technical services. </div> </div> </div> <div class="container"> <div class="row product-data"> <div class="col-lg-6 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4><a href="/t/cd8/antibodies">View all CD8 Antibodies »</a></h4> </div> </div> </div> </div> <div class="border-top border-bottom recommended-products-section" id="recommendation-viewed" data-product_code="NBP1-28237V" data-type=viewed></div> <div id="product-summary" class="py-5 bt-pp-border-bottom" data-section="section-base--product-summary"> <div> <h3>Product Summary for CD8 Antibody (76-2-11) [DyLight 405] </h3> </div> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Immunogen</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> CD8 Antibody (76-2-11) [FITC] was developed against fresh dd miniature swine thymocytes </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Specificity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> CD8 Antibody (76-2-11) recognizes Porcine CD8a, Mr 32-34 kDa </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Clonality</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Host</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Mouse </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Isotype</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> IgG2a Kappa </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Description</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet. </div> </div> </div> </div> <div id="applications" class="py-5 bt-pp-border-bottom" data-anchor-label="Applications" data-section="section-antibody--applications"> <h3>Applications for CD8 Antibody (76-2-11) [DyLight 405] </h3> <div class="mt-5 py-2 container"> <div class="row"> <div class="col-lg-3 border font-weight-bold text-bg-dark py-2 align-top text-md-left text-white bg-dark-bt-blue d-none d-lg-block"> Application </div> <div class="col-lg-9 border align-top py-2 font-weight-bold text-white bg-dark-bt-blue d-none d-lg-block"> Recommended Usage </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Flow Cytometry</h4> </div> <div class="col-lg-9 border align-top py-2"> Optimal dilutions of this antibody should be experimentally determined. </div> </div> </div> <div class="product-data-wrapper mt-5"> Please Note: Optimal dilutions of this antibody should be experimentally determined. </div> </div> <div id="preparation--storage" class="bt-pp-border-bottom py-5" data-section="section-base--preparation-storage"> <h3>Formulation, Preparation, and Storage</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Purification</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Protein A or G purified </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Formulation</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 50mM Sodium Borate </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Preservative</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 0.05% Sodium Azide </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Concentration</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Please see the vial label for concentration. If unlisted please contact technical services. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Shipping</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Stability & Storage</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Store at 4C in the dark. </div> </div> </div> </div> <div id="background" class="py-5 bt-pp-border-bottom" data-section="section-base--background-info"> <h3>Background: CD8</h3> <article class="product-data-wrapper"> CD8, also known as Leu-2 or T8 in human and Lyt2 or Lyt3 in mouse, is a cell surface glycoprotein belonging to the immunoglobulin supergene family (1, 2). CD8 is expressed on cytotoxic T-lymphocytes (T-cells), most thymocytes, between 35-45% of peripheral blood lymphocytes, and a population of natural killer (NK) cells (1, 2). The CD8 molecule consists of disulfide-linked alpha (alpha) and beta (beta) chains that present on T-cells as either CD8alphaalpha homodimers or CD8alphabeta heterodimers (1, 3). Both alpha and beta chains consist of a signaling sequence, an extracellular Ig-like domain, a membrane proximal stalk region, a transmembrane domain, and a cytoplasmic tail (3). Human CD8alpha is processed as 235 amino acids (aa) in length with a theoretical molecular weight of ~26 kDa, while mouse CD8alpha is 247 aa and has a theoretical molecular weight of 27.5 kDa (4, 5). Functionally, CD8 acts as an antigen coreceptor on cytotoxic T-cells and interacts with the major histocompatibility complex (MHC) class I molecules on antigen presenting cells (APCs), mediating cell-cell interactions within the immune system. Conversely, CD4 molecules interact with antigens presented on MHC class II molecules and are activated to become helper T-cells (TH) (1,2). Interestingly, thymocytes can transiently express both CD4 and CD8 during the maturation process (2). Furthermore, the cytoplasmic tail of CD8 has a Lck (lymphocyte-specific protein tyrosine kinase) binding domain where Lck interacts with CD8, initiating a phosphorylation cascade that activates transcription factors and promotes T-cell activation (6). More specifically, CD8alphabeta functions as a T-cell co-receptor, while CD8alphaalpha promotes T-cell survival and differentiation (7). <br/><br/>Given its role in the immune system, CD8-deficiency in T-cells is a hallmark of many diseases and pathologies (8-10). Specifically, CD8+ T-cell deficiency is prevalent in chronic autoimmune diseases including multiple sclerosis, rheumatoid arthritis, ulcerative colitis, Crohn's disease, type 1 diabetes mellitus, and Graves' disease (8). Furthermore, cancers or chronic infection can lead to CD8 T-cell exhaustion as the continual antigen presentation and inflammatory signals eventually cause the CD8+ T-cells to lose functionality (9, 10). However, animal models and clinical studies have suggested that T-cells are capable of being reinvigorated using inhibitory receptor blockade resulting in better disease outcomes and these exhausted T-cells may be a potential therapeutic target (9, 10). <br/><br/>Alternative names for CD8 includes CD antigen: CD8a, CD8 antigen, alpha polypeptide (p32), CD8a molecule, CD8A, Leu2 T-lymphocyte antigen, LEU2, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, and T-lymphocyte differentiation antigen T8/Leu-2. <br/><br/>References<br/><br/>1. Littman D. R. (1987). The structure of the CD4 and CD8 genes. Annual review of immunology. <a href="https://doi.org/10.1146/annurev.iy.05.040187.003021" class="a___link-external" target="_blank">https://doi.org/10.1146/annurev.iy.05.040187.003021</a><br/><br/>2. Naeim F. (2008). Chapter 2- Principles of Immunophenotyping. Hematopathology. <a href="https://doi.org/10.1016/B978-0-12-370607-2.00002-8" class="a___link-external" target="_blank">https://doi.org/10.1016/B978-0-12-370607-2.00002-8</a>. <br/><br/>3. Gao, G. F., & Jakobsen, B. K. (2000). Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunology today. <a href="https://doi.org/10.1016/s0167-5699" class="a___link-external" target="_blank">https://doi.org/10.1016/s0167-5699</a>(00)01750-3<br/><br/>4. UniProt (P01732) <br/><br/>5. UniProt (P01731) <br/><br/>6. Kappes D. J. (2007). CD4 and CD8: hogging all the Lck. Immunity. <a href="https://doi.org/10.1016/j.immuni.2007.11.002" class="a___link-external" target="_blank">https://doi.org/10.1016/j.immuni.2007.11.002</a><br/><br/>7. Gangadharan, D., & Cheroutre, H. (2004). The CD8 isoform CD8alphaalpha is not a functional homologue of the TCR co-receptor CD8alphabeta. Current opinion in immunology. <a href="https://doi.org/10.1016/j.coi.2004.03.015" class="a___link-external" target="_blank">https://doi.org/10.1016/j.coi.2004.03.015</a><br/><br/>8. Pender M. P. (2012). CD8+ T-Cell Deficiency, Epstein-Barr Virus Infection, Vitamin D Deficiency, and Steps to Autoimmunity: A Unifying Hypothesis. Autoimmune diseases. <a href="https://doi.org/10.1155/2012/189096" class="a___link-external" target="_blank">https://doi.org/10.1155/2012/189096</a><br/><br/>9. Kurachi M. (2019). CD8+ T cell exhaustion. Seminars in immunopathology. <a href="https://doi.org/10.1007/s00281-019-00744-5" class="a___link-external" target="_blank">https://doi.org/10.1007/s00281-019-00744-5</a><br/><br/>10. Hashimoto, M., Kamphorst, A. O., Im, S. J., Kissick, H. T., Pillai, R. N., Ramalingam, S. S., Araki, K., & Ahmed, R. (2018). CD8 T Cell Exhaustion in Chronic Infection and Cancer: Opportunities for Interventions. Annual review of medicine. <a href="https://doi.org/10.1146/annurev-med-012017-043208" class="a___link-external" target="_blank">https://doi.org/10.1146/annurev-med-012017-043208</a> </article> <div id="background_wrapper" class="px-3 mt-2"> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Alternate Names</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> CD8, CD8A </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Gene Symbol</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> CD8A </div> </div> </div> <h4 class="mt-3">Additional CD8 Products</h4> <ul class="list-unstyled"> <li><a href="/t/cd8" data-product="CD8">All Products for CD8 </a></li> <li><a href="/t/cd8/cdna-clones" data-application="CD8 cDNA Clones">CD8 cDNA Clones </a></li> <li><a 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</div> </div> </div> <div id="specific-notices" class="py-5" data-section="section-base--specific-notices"> <h3>Product Specific Notices for CD8 Antibody (76-2-11) [DyLight 405] </h3> <p><br><br>DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.</p><p>This product is for research use only and is not approved for use in humans or in clinical diagnosis. 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