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sheet</title> <meta name="description" content="Search results for: cell sheet"> <meta name="keywords" content="cell sheet"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="cell sheet"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4148</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: cell sheet</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3878</span> Vibration of Gamma Graphyne with an Attached Mass</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Win-Jin%20Chang">Win-Jin Chang</a>, <a href="https://publications.waset.org/abstracts/search?q=Haw-Long%20Lee"> Haw-Long Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu-Ching%20Yang"> Yu-Ching Yang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Atomic finite element simulation is applied to investigate the vibration frequency of a single-layer gamma graphyne with an attached mass for the CCCC, SSSS, CFCF, SFSF boundary conditions using the commercial code ANSYS. The fundamental frequencies of the graphyne sheet are compared with the results of the previous study. The results of the comparison are very good in all considered cases. The attached mass causes a shift in the resonant frequency of the graphyne. The frequencies of the single-layer gamma graphyne with an attached mass for different boundary conditions are obtained, and the order based on the boundary condition is CCCC >SSSS > CFCF> SFSF. The highest frequency shift is obtained when the attached mass is located at the center of the graphyne sheet. This is useful for the design of a highly sensitive graphyne-based mass sensor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=graphyne" title="graphyne">graphyne</a>, <a href="https://publications.waset.org/abstracts/search?q=finite%20element%20analysis" title=" finite element analysis"> finite element analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=vibration%20analysis" title=" vibration analysis"> vibration analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=frequency%20shift" title=" frequency shift"> frequency shift</a> </p> <a href="https://publications.waset.org/abstracts/83663/vibration-of-gamma-graphyne-with-an-attached-mass" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/83663.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">212</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3877</span> Soret and Dufour Effect on Variable Viscosity and Thermal Conductivity of an Inclined Magnetic Field with Dissipation in Non-Darcy Porous Medium</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rasaq%20A.%20Kareem">Rasaq A. Kareem</a>, <a href="https://publications.waset.org/abstracts/search?q=Sulyman%20O.%20Salawu"> Sulyman O. Salawu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study of Soret and Dufour effect on variable viscosity and thermal conductivity of an inclined magnetic field with dissipation in non-Darcy porous medium over a continuously stretching sheet for power-law variation in the sheet temperature and concentration are investigated. The viscosity of the fluid flow and thermal conductivity are considered to vary as a function of temperature. The local similarity solutions for different values of the physical parameters are presented for velocity, temperature and concentration. The result shows that variational increase in the values of Soret and Dufour parameters increase the temperature and concentration distribution. Finally, the effects of skin friction, Nusselt and Sherwood numbers which are of physical and engineering interest are considered and discussed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dufour" title="Dufour">Dufour</a>, <a href="https://publications.waset.org/abstracts/search?q=non-Darcy%20Flow" title=" non-Darcy Flow"> non-Darcy Flow</a>, <a href="https://publications.waset.org/abstracts/search?q=Soret" title=" Soret"> Soret</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20conductivity" title=" thermal conductivity"> thermal conductivity</a>, <a href="https://publications.waset.org/abstracts/search?q=variable%20viscosity" title=" variable viscosity"> variable viscosity</a> </p> <a href="https://publications.waset.org/abstracts/51719/soret-and-dufour-effect-on-variable-viscosity-and-thermal-conductivity-of-an-inclined-magnetic-field-with-dissipation-in-non-darcy-porous-medium" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/51719.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">331</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3876</span> Estimation of Greenhouse Gas (GHG) Reductions from Solar Cell Technology Using Bottom-up Approach and Scenario Analysis in South Korea</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jaehyung%20Jung">Jaehyung Jung</a>, <a href="https://publications.waset.org/abstracts/search?q=Kiman%20Kim"> Kiman Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Heesang%20Eum"> Heesang Eum</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Solar cell is one of the main technologies to reduce greenhouse gas (GHG). Thereby, accurate estimation of greenhouse gas reduction by solar cell technology is crucial to consider strategic applications of the solar cell. The bottom-up approach using operating data such as operation time and efficiency is one of the methodologies to improve the accuracy of the estimation. In this study, alternative GHG reductions from solar cell technology were estimated by a bottom-up approach to indirect emission source (scope 2) in Korea, 2015. In addition, the scenario-based analysis was conducted to assess the effect of technological change with respect to efficiency improvement and rate of operation. In order to estimate GHG reductions from solar cell activities in operating condition levels, methodologies were derived from 2006 IPCC guidelines for national greenhouse gas inventories and guidelines for local government greenhouse inventories published in Korea, 2016. Indirect emission factors for electricity were obtained from Korea Power Exchange (KPX) in 2011. As a result, the annual alternative GHG reductions were estimated as 21,504 tonCO2eq, and the annual average value was 1,536 tonCO2eq per each solar cell technology. Those results of estimation showed to be 91% levels versus design of capacity. Estimation of individual greenhouse gases (GHGs) showed that the largest gas was carbon dioxide (CO2), of which up to 99% of the total individual greenhouse gases. The annual average GHG reductions from solar cell per year and unit installed capacity (MW) were estimated as 556 tonCO2eq/yr•MW. Scenario analysis of efficiency improvement by 5%, 10%, 15% increased as much as approximately 30, 61, 91%, respectively, and rate of operation as 100% increased 4% of the annual GHG reductions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bottom-up%20approach" title="bottom-up approach">bottom-up approach</a>, <a href="https://publications.waset.org/abstracts/search?q=greenhouse%20gas%20%28GHG%29" title=" greenhouse gas (GHG)"> greenhouse gas (GHG)</a>, <a href="https://publications.waset.org/abstracts/search?q=reduction" title=" reduction"> reduction</a>, <a href="https://publications.waset.org/abstracts/search?q=scenario" title=" scenario"> scenario</a>, <a href="https://publications.waset.org/abstracts/search?q=solar%20cell" title=" solar cell"> solar cell</a> </p> <a href="https://publications.waset.org/abstracts/57212/estimation-of-greenhouse-gas-ghg-reductions-from-solar-cell-technology-using-bottom-up-approach-and-scenario-analysis-in-south-korea" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57212.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">220</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3875</span> PPRA Regulates DNA Replication Initiation and Cell Morphology in Escherichia coli</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ganesh%20K.%20Maurya">Ganesh K. Maurya</a>, <a href="https://publications.waset.org/abstracts/search?q=Reema%20Chaudhary"> Reema Chaudhary</a>, <a href="https://publications.waset.org/abstracts/search?q=Neha%20Pandey"> Neha Pandey</a>, <a href="https://publications.waset.org/abstracts/search?q=Hari%20S.%20Misra"> Hari S. Misra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> PprA, a pleiotropic protein participating in radioresistance, has been reported for its roles in DNA replication initiation, genome segregation, cell division and DNA repair in polyextremophile Deinococcus radiodurans. Interestingly, expression of deinococcal PprA in E. coli suppresses its growth by reducing the number of colony forming units and provides better resistance against γ-radiation than control. We employed different biochemical and cell biology studies using PprA and its DNA binding/polymerization mutants (K133E & W183R) in E. coli. Cells expressing wild type PprA or its K133E mutant showed reduction in the amount of genomic DNA as well as chromosome copy number in comparison to W183R mutant of PprA and control cells, which suggests the role of PprA protein in regulation of DNA replication initiation in E. coli. Further, E. coli cells expressing PprA or its mutants exhibited different impact on cell morphology than control. Expression of PprA or K133E mutant displayed a significant increase in cell length upto 5 folds while W183R mutant showed cell length similar to uninduced control cells. We checked the interaction of deinococcal PprA and its mutants with E. coli DnaA using Bacterial two-hybrid system and co-immunoprecipitation. We observed a functional interaction of EcDnaA with PprA and K133E mutant but not with W183R mutant of PprA. Further, PprA or K133E mutant has suppressed the ATPase activity of EcDnaA but W183R mutant of PprA failed to do so. These observations suggested that PprA protein regulates DNA replication initiation and cell morphology of surrogate E. coli. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DNA%20replication" title="DNA replication">DNA replication</a>, <a href="https://publications.waset.org/abstracts/search?q=radioresistance" title=" radioresistance"> radioresistance</a>, <a href="https://publications.waset.org/abstracts/search?q=protein-protein%20interaction" title=" protein-protein interaction"> protein-protein interaction</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20morphology" title=" cell morphology"> cell morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=ATPase%20activity" title=" ATPase activity"> ATPase activity</a> </p> <a href="https://publications.waset.org/abstracts/171547/ppra-regulates-dna-replication-initiation-and-cell-morphology-in-escherichia-coli" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171547.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">69</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3874</span> Strip Size Optimization for Spiral Type Actuator Coil Used in Electromagnetic Flat Sheet Forming Experiment </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20A.%20Aleem">M. A. Aleem</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20S.%20Awan"> M. S. Awan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Flat spiral coil for electromagnetic forming system has been modelled in FEMM 4.2 software. Copper strip was chosen as the material for designing the actuator coil. Relationship between height to width ratio (S-factor) of the copper strip and coil’s performance has been studied. Magnetic field intensities, eddy currents, and Lorentz force were calculated for the coils that were designed using six different 'S-factor' values (0.65, 0.75, 1.05, 1.25, 1.54 and 1.75), keeping the cross-sectional area of strip the same. Results obtained through simulation suggest that actuator coil with S-factor ~ 1 shows optimum forming performance as it exerts maximum Lorentz force (84 kN) on work piece. The same coils were fabricated and used for electromagnetic sheet forming experiments. Aluminum 6061 sheets of thickness 1.5 mm have been formed using different voltage levels of capacitor bank. Smooth forming profiles were obtained with dome heights 28, 35 and 40 mm in work piece at 800, 1150 and 1250 V respectively. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=FEM%20modelling" title="FEM modelling">FEM modelling</a>, <a href="https://publications.waset.org/abstracts/search?q=electromagnetic%20forming" title=" electromagnetic forming"> electromagnetic forming</a>, <a href="https://publications.waset.org/abstracts/search?q=spiral%20coil" title=" spiral coil"> spiral coil</a>, <a href="https://publications.waset.org/abstracts/search?q=Lorentz%20force" title=" Lorentz force"> Lorentz force</a> </p> <a href="https://publications.waset.org/abstracts/86282/strip-size-optimization-for-spiral-type-actuator-coil-used-in-electromagnetic-flat-sheet-forming-experiment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86282.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">286</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3873</span> Influence of AgNO3 Treatment on the Flavonolignan Production in Cell Suspension Culture of Silybum marianum (L.) Gaertn</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anna%20Vildov%C3%A1">Anna Vildová</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Hendrychov%C3%A1"> H. Hendrychová</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Kube%C5%A1"> J. Kubeš</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20T%C5%AFmov%C3%A1"> L. Tůmová</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The abiotic elicitation is one of the methods for increasing the secondary metabolites production in plant tissue cultures and it seems to be more effective than traditional strategies. This study verified the use of silver nitrate as elicitor to enhance flavonolignans and flavonoid taxifolin production in suspension culture of Sylibum marianum (L.) Gaertn. Silver nitrate in various concentrations (5.887.10-3 mol/L, 5.887.10-4 mol/L, 5.887.10-5 mol/L) was used as elicitor. The content of secondary metabolites in cell suspension cultures was determined by high performance liquid chromatography. The samples were taken after 6, 12, 24, 48, 72 and 168 hours of treatment. The highest content of taxifolin production (2.2 mg.g-1) in cell suspension culture of Silybum marianum (L.) Gaertn. was detected after silver nitrate (5.887.10-4 mol/L) treatment and 72 h application. Flavonolignans such as silybinA, silybin B, silydianin, silychristin, isosilybin A, isosilybin B were not produced by cell suspension culture of S. marianum after elicitor treatment. Our results show that the secondarymetabolites could be released from S. marianum cells into the nutrient medium by changed permeability of cell wall. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Silybum%20marianum%20%28L.%29%20Gaertn." title="Silybum marianum (L.) Gaertn.">Silybum marianum (L.) Gaertn.</a>, <a href="https://publications.waset.org/abstracts/search?q=elicitation" title=" elicitation"> elicitation</a>, <a href="https://publications.waset.org/abstracts/search?q=silver%20nitrate" title=" silver nitrate"> silver nitrate</a>, <a href="https://publications.waset.org/abstracts/search?q=taxifolin" title=" taxifolin "> taxifolin </a> </p> <a href="https://publications.waset.org/abstracts/11361/influence-of-agno3-treatment-on-the-flavonolignan-production-in-cell-suspension-culture-of-silybum-marianum-l-gaertn" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/11361.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">444</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3872</span> Modeling of the Cellular Uptake of Rigid Nanoparticles: Investigating the Influence of the Adaptation of the Cell’s Mechanical Properties during Endocytosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sarah%20Iaquinta">Sarah Iaquinta</a>, <a href="https://publications.waset.org/abstracts/search?q=Christophe%20Blanquart"> Christophe Blanquart</a>, <a href="https://publications.waset.org/abstracts/search?q=Elena%20Ishow"> Elena Ishow</a>, <a href="https://publications.waset.org/abstracts/search?q=Sylvain%20%20Freour"> Sylvain Freour</a>, <a href="https://publications.waset.org/abstracts/search?q=Frederic%20Jacquemin"> Frederic Jacquemin</a>, <a href="https://publications.waset.org/abstracts/search?q=Shahram%20Khazaie"> Shahram Khazaie</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Nanoparticles have recently emerged as a possible cancer treatment tool. Several formulations have been used to enhance the uptake of these nanoparticles by cancer cells and avoid their immediate clearance when administrated in vivo. Most of the previous studies focus on the investigation of the influence of the mechanical properties of the cell membrane and the particle. However, these studies do not account for the variation of adhesion and tension during the wrapping of the nanoparticle by the membrane. These couplings should be considered since the cell adapts to the interaction with the nanoparticle by, e.g., increasing the number of interactions (consequently leading to an increase of the cell membrane/nanoparticle adhesion) and by reorganizing its cytoskeleton, leading to the releasing of the tension of the cell membrane. The main contribution of this work is the proposal of a novel model for representing the cellular uptake of rigid circular nanoparticles based on an energetic model tailored to take into account the adaptation of the nanoparticle/cell membrane adhesion and of the membrane stress during wrapping. Several coupling models using sigmoidal functions are considered and compared. The study calculations revealed that the results considering constant parameters underestimated the final wrapping degree of the particle by up to 50%. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adhesion" title="adhesion">adhesion</a>, <a href="https://publications.waset.org/abstracts/search?q=cellular%20adaptation" title=" cellular adaptation"> cellular adaptation</a>, <a href="https://publications.waset.org/abstracts/search?q=cellular%20uptake" title=" cellular uptake"> cellular uptake</a>, <a href="https://publications.waset.org/abstracts/search?q=mechanical%20properties" title=" mechanical properties"> mechanical properties</a>, <a href="https://publications.waset.org/abstracts/search?q=tension" title=" tension"> tension</a> </p> <a href="https://publications.waset.org/abstracts/138624/modeling-of-the-cellular-uptake-of-rigid-nanoparticles-investigating-the-influence-of-the-adaptation-of-the-cells-mechanical-properties-during-endocytosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/138624.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">212</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3871</span> Curved Rectangular Patch Array Antenna Using Flexible Copper Sheet for Small Missile Application</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jessada%20Monthasuwan">Jessada Monthasuwan</a>, <a href="https://publications.waset.org/abstracts/search?q=Charinsak%20Saetiaw"> Charinsak Saetiaw</a>, <a href="https://publications.waset.org/abstracts/search?q=Chanchai%20Thongsopa"> Chanchai Thongsopa </a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper presents the development and design of the curved rectangular patch arrays antenna for small missile application. This design uses a 0.1mm flexible copper sheet on the front layer and back layer, and a 1.8mm PVC substrate on a middle layer. The study used a small missile model with 122mm diameter size with speed 1.1 Mach and frequency range on ISM 2.4 GHz. The design of curved antenna can be installation on a cylindrical object like a missile. So, our proposed antenna design will have a small size, lightweight, low cost, and simple structure. The antenna was design and analysis by a simulation result from CST microwave studio and confirmed with a measurement result from a prototype antenna. The proposed antenna has a bandwidth covering the frequency range 2.35-2.48 GHz, the return loss below -10 dB and antenna gain 6.5 dB. The proposed antenna can be applied with a small guided missile effectively. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rectangular%20patch%20arrays" title="rectangular patch arrays">rectangular patch arrays</a>, <a href="https://publications.waset.org/abstracts/search?q=small%20missile%20antenna" title=" small missile antenna"> small missile antenna</a>, <a href="https://publications.waset.org/abstracts/search?q=antenna%20design%20and%20simulation" title=" antenna design and simulation"> antenna design and simulation</a>, <a href="https://publications.waset.org/abstracts/search?q=cylinder%20PVC%20tube" title=" cylinder PVC tube"> cylinder PVC tube</a> </p> <a href="https://publications.waset.org/abstracts/2276/curved-rectangular-patch-array-antenna-using-flexible-copper-sheet-for-small-missile-application" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2276.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">315</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3870</span> Regulation of SHP-2 Activity by Small Molecules for the Treatment of T Cell-Mediated Diseases</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Qiang%20Xu">Qiang Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Xingxin%20Wu"> Xingxin Wu</a>, <a href="https://publications.waset.org/abstracts/search?q=Wenjie%20Guo"> Wenjie Guo</a>, <a href="https://publications.waset.org/abstracts/search?q=Xingqi%20Wang"> Xingqi Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yang%20Sun"> Yang Sun</a>, <a href="https://publications.waset.org/abstracts/search?q=Renxiang%20Tan"> Renxiang Tan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The phosphatase SHP-2 is known to exert regulatory activities on cytokine receptor signaling and the dysregulation of SHP-2 has been implicated in the pathogenesis of a variety of diseases. Here we report several small molecule regulators of SHP-2 for the treatment of T cell-mediated diseases. The new cyclodepsipeptide trichomides A, isolated from the fermentation products of Trichothecium roseum, increased the phosphorylation of SHP-2 in activated T cells, and ameliorated contact dermatitis in mice. The trichomides A’s effects were significantly reversed by using the SHP-2-specific inhibitor PHPS1 or T cell-conditional SHP-2 knockout mice. Another compound is a cerebroside Fusaruside isolated from the endophytic fungus Fusarium sp. IFB-121. Fusaruside also triggered the tyrosine phosphorylation of SHP-2, which provided a possible mean of selectively targeting STAT1 for the treatment of Th1 cell-mediated inflammation and led to the discovery of the non-phosphatase-like function of SHP-2. Namely, the Fusaruside-activated pY-SHP-2 selectively sequestrated the cytosolic STAT1 to prevent its recruitment to IFN-R, which contributed to the improvement of experimental colitis in mice. Blocking the pY-SHP-2-STAT1 interaction, with SHP-2 inhibitor NSC-87877 or using T cells from conditional SHP-2 knockout mice, reversed the effects of fusaruside. Furthermore, the fusaruside’s effect is independent of the phosphatase activity of SHP-2, demonstrating a novel role for SHP-2 in regulating STAT1 signaling and Th1-type immune responses. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=SHP-2" title="SHP-2">SHP-2</a>, <a href="https://publications.waset.org/abstracts/search?q=small%20molecules" title=" small molecules"> small molecules</a>, <a href="https://publications.waset.org/abstracts/search?q=T%20cell" title=" T cell"> T cell</a>, <a href="https://publications.waset.org/abstracts/search?q=T%20cell-mediated%20diseases" title=" T cell-mediated diseases"> T cell-mediated diseases</a> </p> <a href="https://publications.waset.org/abstracts/29058/regulation-of-shp-2-activity-by-small-molecules-for-the-treatment-of-t-cell-mediated-diseases" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29058.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">313</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3869</span> The Molecular Bases of Δβ T-Cell Mediated Antigen Recognition</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eric%20Chabrol">Eric Chabrol</a>, <a href="https://publications.waset.org/abstracts/search?q=Sidonia%20B.G.%20Eckle"> Sidonia B.G. Eckle</a>, <a href="https://publications.waset.org/abstracts/search?q=Renate%20de%20Boer"> Renate de Boer</a>, <a href="https://publications.waset.org/abstracts/search?q=James%20McCluskey"> James McCluskey</a>, <a href="https://publications.waset.org/abstracts/search?q=Jamie%20Rossjohn"> Jamie Rossjohn</a>, <a href="https://publications.waset.org/abstracts/search?q=Mirjam%20H.M.%20Heemskerk"> Mirjam H.M. Heemskerk</a>, <a href="https://publications.waset.org/abstracts/search?q=Stephanie%20Gras"> Stephanie Gras </a> </p> <p class="card-text"><strong>Abstract:</strong></p> αβ and γδ T-cells are disparate T-cell lineages that, via their use of either αβ or γδ T-cell antigen receptors (TCRs) respectively, can respond to distinct antigens. Here we characterise a new population of human T-cells, term δβ T-cells, that express TCRs comprising a TCR-δ variable gene fused to a Joining-α/Constant-α domain, paired with an array of TCR-β chains. We characterised the cellular, functional, biophysical and structural characteristic feature of this new T-cells population that reveal some new insight into TCR diversity. We provide molecular bases of how δβ T-cells can recognise viral peptide presented by Human Leukocyte Antigen (HLA) molecule. Our findings highlight how components from αβ and γδTCR gene loci can recombine to confer antigen specificity thus expanding our understanding of T-cell biology and TCR diversity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=new%20delta-beta%20TCR" title="new delta-beta TCR">new delta-beta TCR</a>, <a href="https://publications.waset.org/abstracts/search?q=HLA" title=" HLA"> HLA</a>, <a href="https://publications.waset.org/abstracts/search?q=viral%20peptide" title=" viral peptide"> viral peptide</a>, <a href="https://publications.waset.org/abstracts/search?q=structural%20immunology" title=" structural immunology"> structural immunology</a> </p> <a href="https://publications.waset.org/abstracts/29618/the-molecular-bases-of-dv-t-cell-mediated-antigen-recognition" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29618.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">425</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3868</span> PPRA Controls DNA Replication and Cell Growth in Escherichia Coli</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ganesh%20K.%20Maurya">Ganesh K. Maurya</a>, <a href="https://publications.waset.org/abstracts/search?q=Reema%20Chaudhary"> Reema Chaudhary</a>, <a href="https://publications.waset.org/abstracts/search?q=Neha%20Pandey"> Neha Pandey</a>, <a href="https://publications.waset.org/abstracts/search?q=Hari%20S.%20Misra"> Hari S. Misra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> PprA, a pleiotropic protein participating in radioresistance, has been reported for its roles in DNA replication initiation, genome segregation, cell division and DNA repair in polyextremophile Deinococcus radiodurans. Interestingly, expression of deinococcal PprA in E. coli suppresses its growth by reducing the number of colony forming units and provide better resistance against γ-radiation than control. We employed different biochemical and cell biology studies using PprA and its DNA binding/polymerization mutants (K133E & W183R) in E. coli. Cells expressing wild type PprA or its K133E mutant showed reduction in the amount of genomic DNA as well as chromosome copy number in comparison to W183R mutant of PprA and control cells, which suggests the role of PprA protein in regulation of DNA replication initiation in E. coli. Further, E. coli cells expressing PprA or its mutants exhibited different impact on cell morphology than control. Expression of PprA or K133E mutant displayed a significant increase in cell length upto 5 folds while W183R mutant showed cell length similar to uninduced control cells. We checked the interaction of deinococcal PprA and its mutants with E. coli DnaA using Bacterial two-hybrid system and co-immunoprecipitation. We observed a functional interaction of EcDnaA with PprA and K133E mutant but not with W183R mutant of PprA. Further, PprA or K133E mutant has suppressed the ATPase activity of EcDnaA but W183R mutant of PprA failed to do so. These observations suggested that PprA protein regulates DNA replication initiation and cell morphology of surrogate E. coli. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DNA%20replication" title="DNA replication">DNA replication</a>, <a href="https://publications.waset.org/abstracts/search?q=radioresistance" title=" radioresistance"> radioresistance</a>, <a href="https://publications.waset.org/abstracts/search?q=protein-protein%20interaction" title=" protein-protein interaction"> protein-protein interaction</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20morphology" title=" cell morphology"> cell morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=ATPase%20activity" title=" ATPase activity"> ATPase activity</a> </p> <a href="https://publications.waset.org/abstracts/171922/ppra-controls-dna-replication-and-cell-growth-in-escherichia-coli" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171922.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">70</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3867</span> Effects of Thermal Radiation on Mixed Convection in a MHD Nanofluid Flow over a Stretching Sheet Using a Spectral Relaxation Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nageeb%20A.%20H.%20Haroun">Nageeb A. H. Haroun</a>, <a href="https://publications.waset.org/abstracts/search?q=Sabyasachi%20Mondal"> Sabyasachi Mondal</a>, <a href="https://publications.waset.org/abstracts/search?q=Precious%20Sibanda"> Precious Sibanda</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The effects of thermal radiation, Soret and Dufour parameters on mixed convection and nanofluid flow over a stretching sheet in the presence of a magnetic field are investigated. The flow is subject to temperature dependent viscosity and a chemical reaction parameter. It is assumed that the nanoparticle volume fraction at the wall may be actively controlled. The physical problem is modelled using systems of nonlinear differential equations which have been solved numerically using a spectral relaxation method. In addition to the discussion on heat and mass transfer processes, the velocity, nanoparticles volume fraction profiles as well as the skin friction coefficient are determined for different important physical parameters. A comparison of current findings with previously published results for some special cases of the problem shows an excellent agreement. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=non-isothermal%20wedge" title="non-isothermal wedge">non-isothermal wedge</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20radiation" title=" thermal radiation"> thermal radiation</a>, <a href="https://publications.waset.org/abstracts/search?q=nanofluid" title=" nanofluid"> nanofluid</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetic%20field" title=" magnetic field"> magnetic field</a>, <a href="https://publications.waset.org/abstracts/search?q=soret%20and%20dufour%20effects" title=" soret and dufour effects"> soret and dufour effects</a> </p> <a href="https://publications.waset.org/abstracts/53060/effects-of-thermal-radiation-on-mixed-convection-in-a-mhd-nanofluid-flow-over-a-stretching-sheet-using-a-spectral-relaxation-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53060.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">235</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3866</span> Regulation of RON-Receptor Tyrosine Kinase Functions by Epstein-Barr-Virus (EBV) Nuclear Antigen 3C</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Roshika%20Tyagi">Roshika Tyagi</a>, <a href="https://publications.waset.org/abstracts/search?q=Shuvomoy%20Banerjee"> Shuvomoy Banerjee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Among various diseases, cancer has become a huge threat to human beings globally. In the context of viral infection, Epstein–Barr virus (EBV) infection is ubiquitous in nature world-wide as well as in India. Recepteur d’Origine Nantais (RON) receptor tyrosine kinase is overexpressed in Lymphoblastoid cell lines (LCLs) but undetectable in primary B-cells. Biologically, RON expression was found to be essential for EBV transformed LCLs proliferation. In our study, we investigated whether EBV latent antigen EBNA3C is playing a crucial role in regulating RON receptor tyrosine kinase function in EBV-induced malignancies. Interestingly, we observed that expression pattern of RON was modulated by EBNA3C in EBV transformed LCLs compared with EBV negative BJAB cell line by PCR and western blot analysis. Moreover, in the absence of EBNA3C, RON expression was found low in western blot and immunofluorescence analysis and cell proliferation rate was significantly reduced in LCLs by cell viability assays. Therefore, our study clearly indicating the potential role of EBNA3C expressed in EBV-infected B-cells for modulating the functions of oncogenic kinases that leads to EBV induced B-cell transformation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title="apoptosis">apoptosis</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20proliferation" title=" cell proliferation"> cell proliferation</a>, <a href="https://publications.waset.org/abstracts/search?q=Epstein%E2%80%93barr%20virus" title=" Epstein–barr virus"> Epstein–barr virus</a>, <a href="https://publications.waset.org/abstracts/search?q=receptor%20tyrosine%20kinase" title=" receptor tyrosine kinase"> receptor tyrosine kinase</a> </p> <a href="https://publications.waset.org/abstracts/66176/regulation-of-ron-receptor-tyrosine-kinase-functions-by-epstein-barr-virus-ebv-nuclear-antigen-3c" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/66176.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">229</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3865</span> The Influence of Polysaccharide Isolated from Morinda citrifolia Fruit to the Growth of Vero, He-La and T47D Cell Lines against Doxorubicin in vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ediati%20Budi%20Cahyono">Ediati Budi Cahyono</a>, <a href="https://publications.waset.org/abstracts/search?q=Triana%20Hertiani"> Triana Hertiani</a>, <a href="https://publications.waset.org/abstracts/search?q=Nauval%20%20Arrazy%20Asawimanda"> Nauval Arrazy Asawimanda</a>, <a href="https://publications.waset.org/abstracts/search?q=Wahyu%20Puji%20Pratomo"> Wahyu Puji Pratomo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Doxorubicin is widely used as a chemotherapeutic drug despite having many side effects. It may cause macrophage dysfunction and decreasing proliferation of lymphocyte. Noni (Morinda citrifolia) fruit which has rich of polysaccharide content has potential as antitumor and immunostimulant effect. The isolation of polysaccharide from Noni fruit has been optimized according to four different methods based on macrophage and lymphocyte activities. We found the highest polysaccharide content from one of the four methods isolation. A method of polysaccharide isolation which has the highest immunostimulant effect was used for further observation as co-chemotherapy. The aim of the study: was to evaluate the isolated polysaccharide from the method of choice as co-chemotherapy of doxorubicin for the growth of Vero, He-La, and T47D cell lines in vitro. The method: in vitro growth assay of Vero, He-La, and T47D cell lines was done using MTT-reduction method, and apoptosis test was done by double staining method to evaluate the induction apoptotic effect of the combination. Every group was treated with doxorubicin and isolated polysaccharide from method of choice with 4 variances of concentrations (25 µg/ml, 50 µg/ml, 100 µg/ml and 200 µg/ml) a long with negative control (doxorubicin only) and normal control (without doxorubicin or polysaccharide administration). Results: The combination of polysaccharide fraction in the concentration of 100μg/ml with 2μmol of doxorubicin against He-La and T47D cell lines influenced the highest cytotoxic effect by suppressing cell viability comparing with doxorubicin only. The combination of polysaccharide fraction in the concentration of 100μg/ml with 2μmol of doxorubicin-induced apoptotic effect the He-La cell line comparing with doxorubicin only. The result of the study: it can be concluded that the combination of polysaccharide fraction and doxorubicin effect more selective toward He-La and T47D cell lines than to Vero cell line. It can be suggested isolated polysaccharide from the method of choice has co-chemotherapy activity against doxorubicin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=polysaccharide" title="polysaccharide">polysaccharide</a>, <a href="https://publications.waset.org/abstracts/search?q=noni%20fruit" title=" noni fruit"> noni fruit</a>, <a href="https://publications.waset.org/abstracts/search?q=doxorubicin" title=" doxorubicin"> doxorubicin</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer%20cell%20lines" title=" cancer cell lines"> cancer cell lines</a>, <a href="https://publications.waset.org/abstracts/search?q=vero%20cell%20line" title=" vero cell line"> vero cell line</a> </p> <a href="https://publications.waset.org/abstracts/67329/the-influence-of-polysaccharide-isolated-from-morinda-citrifolia-fruit-to-the-growth-of-vero-he-la-and-t47d-cell-lines-against-doxorubicin-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/67329.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">251</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3864</span> A Comparison of Single Point Incremental Forming Formability between Carbon Steel and Stainless Steel</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kittiphat%20Rattanachan">Kittiphat Rattanachan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The sheet metal forming process, the raw material mechanical properties are important parameters. This paper is to compare the wall’s incline angle or formability of SS 400 steel and SUS 304 stainless steel in single point incremental forming. The two materials are ferrous base alloy, which have the different cell unit, mechanical property and chemical composition. They were forming into cone shape specimens 100 mm diameter with different wall’s incline angle: 90o, 75o, and 60o. The investigation, the specimens were forming until the surface fracture was occurred. The experimental result showed that both materials with the smaller wall’s incline angle, the higher formability. The formability limited of the ferrous base alloy was approx. 60o wall’s incline angle. By nature, SS 400 was higher formability than SUS 304. This result could be used as the initial utilized data in designing the single point incremental forming parts. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=NC%20incremental%20forming" title="NC incremental forming">NC incremental forming</a>, <a href="https://publications.waset.org/abstracts/search?q=single%20point%20incremental%20forming" title=" single point incremental forming"> single point incremental forming</a>, <a href="https://publications.waset.org/abstracts/search?q=wall%20incline%20angle" title=" wall incline angle"> wall incline angle</a>, <a href="https://publications.waset.org/abstracts/search?q=formability" title=" formability"> formability</a> </p> <a href="https://publications.waset.org/abstracts/11732/a-comparison-of-single-point-incremental-forming-formability-between-carbon-steel-and-stainless-steel" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/11732.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">344</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3863</span> The Stability and Performances of Terminalia Catappa L. Dye-Sensitized Solar Cell</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20O.%20Boyo">A. O. Boyo</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20T.%20Akinwunmi"> A. T. Akinwunmi </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The effect of extracting solvent and adjustment of pHs on the stability of Terminalia catappa L. dye-sensitized solar cell was investigated. We introduced ZnO as an alternative to TiO2 in the dye sensitized solar cells (DSSCs) due to its band gap similar to TiO2, higher electron mobility, and flexible procedures of preparations. Dye-sensitized solar cells (DSSCs) based on Terminalia catappa L. was extracted in water (A), ethanol (B) and the mixture of ethanol and water in the ratio 1:1by volume (C). The best performance Solar cells sensitized was from extracts A and achieved up to Jsc 1.51 mAcm−2, Voc 0.75V, FF 0.88 and η 0.63%. We notice that as pHs decreases there is the increase in DSSC efficiency. There is Long period stability in efficiency of the cells prepared using A than in C and a fair stability in efficiency of B cell. The results obtained with extracts B and C confirmed that Ethanol with water could not be considered as a suitable solvent for the extraction of natural dye. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=zinc%20oxide" title="zinc oxide">zinc oxide</a>, <a href="https://publications.waset.org/abstracts/search?q=dye-sensitized%20solar%20cell" title=" dye-sensitized solar cell"> dye-sensitized solar cell</a>, <a href="https://publications.waset.org/abstracts/search?q=terminalia%20catappa%20L." title=" terminalia catappa L."> terminalia catappa L.</a>, <a href="https://publications.waset.org/abstracts/search?q=TiO2" title=" TiO2 "> TiO2 </a> </p> <a href="https://publications.waset.org/abstracts/37893/the-stability-and-performances-of-terminalia-catappa-l-dye-sensitized-solar-cell" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37893.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">402</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3862</span> The Improved Biofuel Cell for Electrical Power Generation from Wastewaters</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20S.%20Kilic">M. S. Kilic</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Korkut"> S. Korkut</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Hazer"> B. Hazer</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Newly synthesized Polypropylene-g-Polyethylene glycol polymer was first time used for a compartment-less enzymatic fuel cell. Working electrodes based on Polypropylene-g-Polyethylene glycol were operated as unmediated and mediated system (with ferrocene and gold/cobalt oxide nanoparticles). Glucose oxidase and bilirubin oxidase was selected as anodic and cathodic enzyme, respectively. Glucose was used as fuel in a single-compartment and membrane-less cell. Maximum power density was obtained as 0.65 nW cm-2, 65 nW cm-2, and 23500 nW cm-2 from the unmediated, ferrocene and gold/cobalt oxide modified polymeric film, respectively. Power density was calculated to be ~16000 nW cm-2 for undiluted wastewater sample with gold/cobalt oxide nanoparticles including system. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bilirubin%20oxidase" title="bilirubin oxidase">bilirubin oxidase</a>, <a href="https://publications.waset.org/abstracts/search?q=enzymatic%20fuel%20cell" title=" enzymatic fuel cell"> enzymatic fuel cell</a>, <a href="https://publications.waset.org/abstracts/search?q=glucose%20oxidase" title=" glucose oxidase"> glucose oxidase</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title=" nanoparticles"> nanoparticles</a> </p> <a href="https://publications.waset.org/abstracts/16725/the-improved-biofuel-cell-for-electrical-power-generation-from-wastewaters" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16725.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">263</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3861</span> Isolation and Expansion of Human Periosteum-Derived Mesenchymal Stem Cells in Defined Serum-Free Culture Medium</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ainur%20Mukhambetova">Ainur Mukhambetova</a>, <a href="https://publications.waset.org/abstracts/search?q=Miras%20Karzhauov"> Miras Karzhauov</a>, <a href="https://publications.waset.org/abstracts/search?q=Vyacheslav%20Ogay"> Vyacheslav Ogay</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Mesenchymal stem cells (MSCs) have the capacity to be differentiated into several cell lineages and are a promising source for cell therapy and tissue engineering. However, currently most MSCs culturing protocols use media supplemented with fetal bovine serum (FBS), which limits their application in clinic due to the possibility of zoonotic infections, contamination and immunological reactions. Consequently, formulating effective serum free culture medium becomes one of the important problems in contemporary cell biotechnology. Objectives: The aim of this study was to define an optimal serum-free medium for culturing of periosteum derived MSCs. Materials and methods: The MSCs were extracted from human periosteum and transferred to the culture flasks pretreated with CELLstart™. Immunophenotypic characterization, proliferation and in vitro differentiation of cells grown on STEM PRO® MSC SFM were compared to the cells cultured in the standard FBS containing media. Chromosome analysis and flow cytometry were also performed. Results: We have shown that cells were grown on STEM PRO® MSC SFM retained all the morphological, immunophenotypic (CD73, CD90, CD105, vimentin and Stro-1) and cell differentiation characteristics specific to MSCs. Chromosome analysis indicated no anomalies in the chromosome structure. Flow cytometry showed a high expression of cell adhesion molecules CD44 (98,8%), CD90 (97,4%), CD105 (99,1%). In addition, we have shown that cell is grown on STEM PRO® MSC SFM have higher proliferation capacity compared to cell expanded on standard FBS containing the medium. Conclusion: We have shown that STEM PRO® MSC SFM is optimal for culturing periosteum derived human MSCs which subsequently can be safely used in cell therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cell%20technologies" title="cell technologies">cell technologies</a>, <a href="https://publications.waset.org/abstracts/search?q=periosteum-derived%20MSCs" title=" periosteum-derived MSCs"> periosteum-derived MSCs</a>, <a href="https://publications.waset.org/abstracts/search?q=regenerative%20medicine" title=" regenerative medicine"> regenerative medicine</a>, <a href="https://publications.waset.org/abstracts/search?q=serum-free%20medium" title=" serum-free medium"> serum-free medium</a> </p> <a href="https://publications.waset.org/abstracts/31395/isolation-and-expansion-of-human-periosteum-derived-mesenchymal-stem-cells-in-defined-serum-free-culture-medium" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31395.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">298</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3860</span> The Effect of Thymoquinone and Sorafenib Combination on Hepatocellular Carcinoma Cell Line</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nabila%20N.%20El-Maraghy">Nabila N. El-Maraghy</a>, <a href="https://publications.waset.org/abstracts/search?q=Amany%20Essa"> Amany Essa</a>, <a href="https://publications.waset.org/abstracts/search?q=Yousra%20Abdel%E2%80%93Mottaleb"> Yousra Abdel–Mottaleb</a>, <a href="https://publications.waset.org/abstracts/search?q=Nada%20Ismail"> Nada Ismail</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The use of combination of chemotherapy and natural products to influence the cell death with low doses of chemotherapeutic agents and few side effects has recently emerged as a new method of cancer therapy. Aim: Evaluation the modulatory effect of Thymoquinone on HepG2 cells treated with Sorafenib. Methods: Hepatocellular Carcinoma HepG2 cell line was treated with Sorafenib and TQ individually and in combination. The effect of these treatments on cell viability (MTT assay), apoptosis (Expression of Caspase-3) and oxidative markers (GSH content and extent of lipid peroxidation) was determined. Results: When compared the effect of both agents alone and the combination of the IC50 of Sorafenib and the IC50 TQ, the combination resulted in reduction of cell inhibition and apoptosis and antagonize their actions on GSH content and extent of lipid peroxidation which are increased. This study showed potent anti-tumor activity of both TQ and Sorafenib separately on HepG2 but upon combination surprisingly they interacted and give antagonistic effect. Conclusion: Co-treatment resulted in antagonistic interaction between Sorafenib and Thymoquinone. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antagonism" title="antagonism">antagonism</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatocellular%20carcinoma" title=" hepatocellular carcinoma"> hepatocellular carcinoma</a>, <a href="https://publications.waset.org/abstracts/search?q=sorafenib" title=" sorafenib"> sorafenib</a>, <a href="https://publications.waset.org/abstracts/search?q=thymoquinone" title=" thymoquinone "> thymoquinone </a> </p> <a href="https://publications.waset.org/abstracts/48191/the-effect-of-thymoquinone-and-sorafenib-combination-on-hepatocellular-carcinoma-cell-line" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/48191.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">554</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3859</span> Evaluation on Estrogenic Effects of Diisononyl Adipate (DiNA) on MCF-7 Human Breast Cancer Cell Lines</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shih-cheng%20Li">Shih-cheng Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Ming-Yi%20Chung"> Ming-Yi Chung</a>, <a href="https://publications.waset.org/abstracts/search?q=Mei-Lien%20Chen"> Mei-Lien Chen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Plasticizers, such as phthalates and adipates, were substances added to a material that provided flexibility and durability to plastics such as polyvinyl chloride (PVC). Phthalates were generally recognized as an endocrine disrupter due to their estrogenic and anti-androgenic activities. Phthalates had the capacity to bind to estrogen receptors, and hence they might prolong menstrual cycles and increase the proportion of premature menopause. Recently, adipates such as di-2-ethylhexyl adipate (DEHA) and di-isononyl adipate (DiNA) had replaced phthalates and were now used for food packaging. Methods: MCF-7 cell lines were treated with di-2-ethylhexyl phthalate (DEHP), di- 2-ethylhexyl adipate (DEHA), or di-isononyl adipate (DiNA) (10-6 , 10-5 , and 10-4 mol/l), using 17β-estradiol (10-8 mol/l) as a positive control. After incubations of 24, 48, 72, and 96 hours, the cells were tested using the alamarBlue assay. Results: The alamarBlue assay revealed that cell proliferation significantly increased after treatments of DEHP and DEHA for 24 hours at a concentration of 10-6, 10-5, and 10-4 mol/l. After more than 48 hours, cell proliferations in DEHP at 10-6, 10-5, and 10-4 mol/l significantly decreased compared to the control group. Conclusions: The present study demonstrates that adipates, as well as phthalates, were capable of inducing cell proliferation. We further used MDA-MB-231 cell lines to confirm that the proliferation effect was generated through binding to estrogen receptors. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=MCF-7" title="MCF-7">MCF-7</a>, <a href="https://publications.waset.org/abstracts/search?q=phthalate" title=" phthalate"> phthalate</a>, <a href="https://publications.waset.org/abstracts/search?q=adipate" title=" adipate"> adipate</a>, <a href="https://publications.waset.org/abstracts/search?q=endocrine%20disrupter" title=" endocrine disrupter"> endocrine disrupter</a> </p> <a href="https://publications.waset.org/abstracts/42355/evaluation-on-estrogenic-effects-of-diisononyl-adipate-dina-on-mcf-7-human-breast-cancer-cell-lines" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42355.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">296</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3858</span> BingleSeq: A User-Friendly R Package for Single-Cell RNA-Seq Data Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Quan%20Gu">Quan Gu</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20%20Dimitrov"> Daniel Dimitrov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> BingleSeq was developed as a shiny-based, intuitive, and comprehensive application that enables the analysis of single-Cell RNA-Sequencing count data. This was achieved via incorporating three state-of-the-art software packages for each type of RNA sequencing analysis, alongside functional annotation analysis and a way to assess the overlap of differential expression method results. At its current state, the functionality implemented within BingleSeq is comparable to that of other applications, also developed with the purpose of lowering the entry requirements to RNA Sequencing analyses. BingleSeq is available on GitHub and will be submitted to R/Bioconductor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioinformatics" title="bioinformatics">bioinformatics</a>, <a href="https://publications.waset.org/abstracts/search?q=functional%20annotation%20analysis" title=" functional annotation analysis"> functional annotation analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=single-cell%20RNA-sequencing" title=" single-cell RNA-sequencing"> single-cell RNA-sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=transcriptomics" title=" transcriptomics"> transcriptomics</a> </p> <a href="https://publications.waset.org/abstracts/120198/bingleseq-a-user-friendly-r-package-for-single-cell-rna-seq-data-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/120198.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">205</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3857</span> Vibration Measurements of Single-Lap Cantilevered SPR Beams</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xiaocong%20He">Xiaocong He</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Self-pierce riveting (SPR) is a new high-speed mechanical fastening technique which is suitable for point joining dissimilar sheet materials, as well as coated and pre-painted sheet materials. Mechanical structures assembled by SPR are expected to possess a high damping capacity. In this study, experimental measurement techniques were proposed for the prediction of vibration behavior of single-lap cantilevered SPR beams. The dynamic test software and the data acquisition hardware were used in the experimental measurement of the dynamic response of the single-lap cantilevered SPR beams. Free and forced vibration behavior of the single-lap cantilevered SPR beams was measured using the LMS CADA-X experimental modal analysis software and the LMS-DIFA Scadas II data acquisition hardware. The frequency response functions of the SPR beams of different rivet number were compared. The main goal of the paper is to provide a basic measuring method for further research on vibration based non-destructive damage detection in single-lap cantilevered SPR beams. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=self-piercing%20riveting" title="self-piercing riveting">self-piercing riveting</a>, <a href="https://publications.waset.org/abstracts/search?q=dynamic%20response" title=" dynamic response"> dynamic response</a>, <a href="https://publications.waset.org/abstracts/search?q=experimental%20measurement" title=" experimental measurement"> experimental measurement</a>, <a href="https://publications.waset.org/abstracts/search?q=frequency%20response%20functions" title=" frequency response functions"> frequency response functions</a> </p> <a href="https://publications.waset.org/abstracts/21181/vibration-measurements-of-single-lap-cantilevered-spr-beams" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21181.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">429</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3856</span> Studying the Effect of Silicon Substrate Intrinsic Carrier Concentration on Performance of ZnO/Si Solar Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Syed%20Sadique%20Anwer%20Askari">Syed Sadique Anwer Askari</a>, <a href="https://publications.waset.org/abstracts/search?q=Mukul%20Kumar%20Das"> Mukul Kumar Das</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Zinc Oxide (ZnO) solar cells have drawn great attention due to the enhanced efficiency and low-cost fabrication process. In this study, ZnO thin film is used as the active layer, hole blocking layer, antireflection coating (ARC) as well as transparent conductive oxide. To improve the conductivity of ZnO, top layer of ZnO is doped with aluminum, for top contact. Intrinsic carrier concentration of silicon substrate plays an important role in enhancing the power conversion efficiency (PCE) of ZnO/Si solar cell. With the increase of intrinsic carrier concentration PCE decreased due to increase in dark current in solar cell. At 80nm ZnO and 160µm Silicon substrate thickness, power conversion efficiency of 26.45% and 21.64% is achieved with intrinsic carrier concentration of 1x109/cm3, 1.4x1010/cm3 respectively. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hetero-junction%20solar%20cell" title="hetero-junction solar cell">hetero-junction solar cell</a>, <a href="https://publications.waset.org/abstracts/search?q=solar%20cell" title=" solar cell"> solar cell</a>, <a href="https://publications.waset.org/abstracts/search?q=substrate%20intrinsic%20carrier%20concentration" title=" substrate intrinsic carrier concentration"> substrate intrinsic carrier concentration</a>, <a href="https://publications.waset.org/abstracts/search?q=ZnO%2FSi" title=" ZnO/Si"> ZnO/Si</a> </p> <a href="https://publications.waset.org/abstracts/61939/studying-the-effect-of-silicon-substrate-intrinsic-carrier-concentration-on-performance-of-znosi-solar-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61939.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">601</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3855</span> Mathematical Modelling of the Effect of Glucose on Pancreatic Alpha-Cell Activity </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Karen%20K.%20Perez-Ramirez">Karen K. Perez-Ramirez</a>, <a href="https://publications.waset.org/abstracts/search?q=Genevieve%20Dupont"> Genevieve Dupont</a>, <a href="https://publications.waset.org/abstracts/search?q=Virginia%20Gonzalez-Velez"> Virginia Gonzalez-Velez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Pancreatic alpha-cells participate on glucose regulation together with beta cells. They release glucagon hormone when glucose level is low to stimulate gluconeogenesis from the liver. As other excitable cells, alpha cells generate Ca2+ and metabolic oscillations when they are stimulated. It is known that the glucose level can trigger or silence this activity although it is not clear how this occurs in normal and diabetic people. In this work, we propose an electric-metabolic mathematical model implemented in Matlab to study the effect of different glucose levels on the electrical response and Ca2+ oscillations of an alpha cell. Our results show that Ca2+ oscillations appear in opposite phase with metabolic oscillations in a window of glucose values. The model also predicts a direct relationship between the level of glucose and the intracellular adenine nucleotides showing a self-regulating pathway for the alpha cell. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ca2%2B%20oscillations" title="Ca2+ oscillations">Ca2+ oscillations</a>, <a href="https://publications.waset.org/abstracts/search?q=mathematical%20model" title=" mathematical model"> mathematical model</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolic%20oscillations" title=" metabolic oscillations"> metabolic oscillations</a>, <a href="https://publications.waset.org/abstracts/search?q=pancreatic%20alpha%20cell" title=" pancreatic alpha cell"> pancreatic alpha cell</a> </p> <a href="https://publications.waset.org/abstracts/96002/mathematical-modelling-of-the-effect-of-glucose-on-pancreatic-alpha-cell-activity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96002.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">178</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3854</span> Chemical Bath Deposition Technique of CdS Used in Closed Space Sublimation of CdTe Solar Cell</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Z.%20Mahmood">Z. Mahmood</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20U.%20Babar"> F. U. Babar</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Naz"> S. Naz</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20U.%20Rehman"> H. U. Rehman</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cadmium Sulphide (CdS) was deposited on a Tec 15 glass substrate with the help of CBD (chemical bath deposition process) and then cadmium telluride CdTe was deposited on CdS with the help of CSS (closed spaced sublimation technique) for the construction of a solar cell. The thicknesses of all the deposited materials were measured with the help of Ellipsometry. The IV graphs were drawn in order to observe the current voltage output. The efficiency of the cell was graphed with the fill factor as well (graphs not given here). The efficiency came out to be approximately 16.5 % and the CIGS (copper-indium–gallium-selenide) maximum efficiency is 20 %. The efficiency of a solar cell can further be enhanced by adapting quality materials, good experimental devices and proper procedures. The grain size was analyzed with the help of scanning electron microscope using RBS (Rutherford backscattering spectroscopy). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chemical%20Bath%20Deposition%20Technique%20%28CBD%29" title="Chemical Bath Deposition Technique (CBD)">Chemical Bath Deposition Technique (CBD)</a>, <a href="https://publications.waset.org/abstracts/search?q=cadmium%20sulphide%20%28CdS%29" title=" cadmium sulphide (CdS)"> cadmium sulphide (CdS)</a>, <a href="https://publications.waset.org/abstracts/search?q=CdTe" title=" CdTe"> CdTe</a>, <a href="https://publications.waset.org/abstracts/search?q=CSS%20%28Closed%20Space%20Sublimation%29" title=" CSS (Closed Space Sublimation)"> CSS (Closed Space Sublimation)</a> </p> <a href="https://publications.waset.org/abstracts/49998/chemical-bath-deposition-technique-of-cds-used-in-closed-space-sublimation-of-cdte-solar-cell" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/49998.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">364</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3853</span> Promotion of Lipid Syntheses of Microalgae by Microfluidic-Assisted Membrane Distortion</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Seul%20Ki%20Min">Seul Ki Min</a>, <a href="https://publications.waset.org/abstracts/search?q=Gwang%20Heum%20Yoon"> Gwang Heum Yoon</a>, <a href="https://publications.waset.org/abstracts/search?q=Jung%20Hyun%20Joo"> Jung Hyun Joo</a>, <a href="https://publications.waset.org/abstracts/search?q=Hwa%20Sung%20Shin"> Hwa Sung Shin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cellular membrane distortion is known as a factor to change intracellular signaling. However, progress of relevant studies is difficult because there are no facilities that can control membrane distortion finely. In this study, we developed microfluidic device which can inflict mechanical stress on cell membrane of Chlamydomonas reinhardtii using regular height of the channels. And cellular physiological changes were analyzed from cells cultured in the device. Excessive calcium ion influx through into cytoplasm was induced from mechanical stress. The results revealed that compressed cells had up-regulated Mat3 mRNA which regulates cell size and cell cycle from a prolonged G1 phase. Additionally, TAG used for the production of biodiesel was raised rapidly from 4 h after compression. Taken together, membrane distortion can be considered as an attractive inducer for biofuel production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mechanical%20stress" title="mechanical stress">mechanical stress</a>, <a href="https://publications.waset.org/abstracts/search?q=membrane%20distortion" title=" membrane distortion"> membrane distortion</a>, <a href="https://publications.waset.org/abstracts/search?q=Chlamydomonas%20reinhardtii" title=" Chlamydomonas reinhardtii"> Chlamydomonas reinhardtii</a>, <a href="https://publications.waset.org/abstracts/search?q=deflagellation" title=" deflagellation"> deflagellation</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20cycle" title=" cell cycle"> cell cycle</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid%20metabolism" title=" lipid metabolism"> lipid metabolism</a> </p> <a href="https://publications.waset.org/abstracts/7818/promotion-of-lipid-syntheses-of-microalgae-by-microfluidic-assisted-membrane-distortion" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/7818.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">375</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3852</span> Effect of Machining Induced Microstructure Changes on the Edge Formability of Titanium Alloys at Room Temperature</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=James%20S.%20Kwame">James S. Kwame</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Yakushina"> E. Yakushina</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Blackwell"> P. Blackwell</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The challenges in forming titanium alloys at room temperature are well researched and are linked both to the limitations imposed by the basic crystal structure and their ability to form texture during plastic deformation. One major issue of concern for the sheet forming of titanium alloys is their high sensitivity to surface inhomogeneity. Various machining processes are utilised in preparing sheet hole edges for edge flanging applications. However, the response of edge forming tendencies of titanium to different edge surface finishes is not well investigated. The hole expansion test is used in this project to elucidate the impact of abrasive water jet (AWJ) and electro-discharge machining (EDM) cutting techniques on the edge formability of CP-Ti (Grade 2) and Ti-3Al-2.5V alloys at room temperature. The results show that the quality of the edge surface finish has a major effect on the edge formability of the materials. The work also found that the variations in the edge forming performance are mainly the result of the influence of machining induced edge surface defects. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=titanium%20alloys" title="titanium alloys">titanium alloys</a>, <a href="https://publications.waset.org/abstracts/search?q=hole%20expansion%20test" title=" hole expansion test"> hole expansion test</a>, <a href="https://publications.waset.org/abstracts/search?q=edge%20formability" title=" edge formability"> edge formability</a>, <a href="https://publications.waset.org/abstracts/search?q=non-conventional%20machining" title=" non-conventional machining"> non-conventional machining</a> </p> <a href="https://publications.waset.org/abstracts/110917/effect-of-machining-induced-microstructure-changes-on-the-edge-formability-of-titanium-alloys-at-room-temperature" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/110917.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">137</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3851</span> Study of Hybrid Cells Based on Perovskite Materials Using Oghmasimultion</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nadia%20Bachir%20%28Dahmani%29">Nadia Bachir (Dahmani)</a>, <a href="https://publications.waset.org/abstracts/search?q=Fatima%20Zohra%20Otmani"> Fatima Zohra Otmani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Due to its interesting optoelectronic properties, methylammonium perovskite CH3NH3PbI3 is used as the active layer in the development of several solar cells. In this work, the hybrid (organic-inorganic) cell with the architecture FTO/pedotpss/CH3NH3PbI3/pcdtbt/Al is simulated using the Organic and Hybrid Material Nano Simulation Tool (OghmaNano). We studied the influence of certain parameters, such as thickness, on the characteristics of the solar cell. The effect of the device temperature was also investigated. The photovoltaic characteristic curves, such as current-voltage (j-V), are presented in this work. The optimized final parameters are Voc = 0.947 V, FF = 0.8034%, and PCE = 23.16%. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=OghmaNano%20software" title="OghmaNano software">OghmaNano software</a>, <a href="https://publications.waset.org/abstracts/search?q=hybrid%20perovskite%20cell" title=" hybrid perovskite cell"> hybrid perovskite cell</a>, <a href="https://publications.waset.org/abstracts/search?q=CH3NH3PbI3" title=" CH3NH3PbI3"> CH3NH3PbI3</a>, <a href="https://publications.waset.org/abstracts/search?q=conversion%20efficiency" title=" conversion efficiency"> conversion efficiency</a> </p> <a href="https://publications.waset.org/abstracts/193533/study-of-hybrid-cells-based-on-perovskite-materials-using-oghmasimultion" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193533.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">14</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3850</span> Multilayered Assembly of Gelatin on Nanofibrous Matrix for 3-D Cell Cultivation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ji%20Un%20Shin">Ji Un Shin</a>, <a href="https://publications.waset.org/abstracts/search?q=Wei%20Mao"> Wei Mao</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyuk%20Sang%20Yoo"> Hyuk Sang Yoo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Electrospinning is a versatile tool for fabricating nano-structured polymeric materials. Gelatin hydrogels are considered to be a good material for cell cultivation because of high water swellability as well as good biocompatibility. Three-dimensional (3-D) cell cultivation is a desirable method of cell cultivation for preparing tissues and organs because cell-to-cell interactions or cell-to-matrix interactions can be much enhanced through this approach. For this reason, hydrogels were widely employed as tissue scaffolds because they can support cultivating cells and tissue in multi-dimensions. Major disadvantages of hydrogel-based cell cultivation include low mechanical properties, lack of topography, which should be enhanced for successful tissue engineering. Herein we surface-immobilized gelatin on the surface of nanofibrous matrix for 3-D cell cultivation in topographical cues added environments. Electrospun nanofibers were electrospun with injection of poly(caprolactone) through a single nozzle syringe. Electrospun meshes were then chopped up with a high speed grinder to fine powders. This was hydrolyzed in optimized concentration of sodium hydroxide solution from 1 to 6 hours and harvested by centrifugation. The freeze-dried powders were examined by scanning electron microscopy (SEM) for revealing the morphology and fibrilar shaped with a length of ca. 20um was observed. This was subsequently immersed in gelatin solution for surface-coating of gelatin, where the process repeated up to 10 times for obtaining desirable coating of gelatin on the surface. Gelatin-coated nanofibrils showed high waterswellability in comparison to the unmodified nanofibrils, and this enabled good dispersion properties of the modified nanofibrils in aqueous phase. The degree of water-swellability was increased as the coating numbers of gelatin increased, however, it did not any meaning result after 10 times of gelatin coating process. Thus, by adjusting the gelatin coating times, we could successfully control the degree of hydrophilicity and water-swellability of nanofibrils. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nano" title="nano">nano</a>, <a href="https://publications.waset.org/abstracts/search?q=fiber" title=" fiber"> fiber</a>, <a href="https://publications.waset.org/abstracts/search?q=cell" title=" cell"> cell</a>, <a href="https://publications.waset.org/abstracts/search?q=tissue" title=" tissue"> tissue</a> </p> <a href="https://publications.waset.org/abstracts/86571/multilayered-assembly-of-gelatin-on-nanofibrous-matrix-for-3-d-cell-cultivation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86571.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">167</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3849</span> A Biomechanical Perfusion System for Microfluidic 3D Bioprinted Structure</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Dimitri">M. Dimitri</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Ricci"> M. Ricci</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Bigi"> F. Bigi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Romiti"> M. Romiti</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Corvi"> A. Corvi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Tissue engineering has reached a significant milestone with the integration of 3D printing for the creation of complex bioconstructs equipped with vascular networks, crucial for cell maintenance and growth. This study aims to demonstrate the effectiveness of a portable microperfusion system designed to adapt dynamically to the evolving conditions of cell growth within 3D-printed bioconstructs. The microperfusion system was developed to provide a constant and controlled flow of nutrients and oxygen through the integrated vessels in the bioconstruct, replicating in vivo physiological conditions. Through a series of preliminary experiments, we evaluated the system's ability to maintain a favorable environment for cell proliferation and differentiation. Measurements of cell density and viability were performed to monitor the health and functionality of the tissue over time. Preliminary results indicate that the portable microperfusion system not only supports but optimizes cell growth, effectively adapting to changes in metabolic needs during the bioconstruct maturation process. This research opens perspectives in tissue engineering, demonstrating that a portable microperfusion system can be successfully integrated into 3D-printed bioconstructs, promoting sustainable and uniform cell growth. The implications of this study are far-reaching, with potential applications in regenerative medicine and pharmacological research, providing a platform for the development of functional and complex tissues. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofabrication" title="biofabrication">biofabrication</a>, <a href="https://publications.waset.org/abstracts/search?q=microfluidic%20perfusion%20system" title=" microfluidic perfusion system"> microfluidic perfusion system</a>, <a href="https://publications.waset.org/abstracts/search?q=4D%20bioprinting" title=" 4D bioprinting"> 4D bioprinting</a> </p> <a href="https://publications.waset.org/abstracts/189002/a-biomechanical-perfusion-system-for-microfluidic-3d-bioprinted-structure" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/189002.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 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