CINXE.COM

ATG5 Antibody - BSA Free (NB110-53818): Novus Biologicals

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View knockout-validated Rabbit Polyclonal anti-ATG5 Antibody - BSA Free (NB110-53818). Validated Applications: WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP, MS, WB, KD, KO. Validated Species: Human, Mouse, Rat + more. Validated by: Genetic Strategies. Sample size available. Range of directly conjugated antibodies available." /> <meta property="og:description" content="Cited in 285 publications. View knockout-validated Rabbit Polyclonal anti-ATG5 Antibody - BSA Free (NB110-53818). Validated Applications: WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP, MS, WB, KD, KO. Validated Species: Human, Mouse, Rat + more. Validated by: Genetic Strategies. Sample size available. 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<td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue">&nbsp;</div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="3" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nb110-53818_rabbit-polyclonal-atg5-antibody-30820241044550.jpg" class="top_images" rel="gallery" title="Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P 0.9999), as compared to CQ (50 uM) only, a dose that did not completely inhibit autophagy. Data represent mean +- SEM. (H) N27 cells were treated with 62.5 or 125 uM of MnCl2 for 24h, then incubated with LysoSensor Yellow/Blue. The ratio of acidic (yellow) vs. neutral (blue) pH of lysosomes were quantified using a plate reader. (I) N27 cells were treated with Mn 125 uM, CQ50 uM or both for 24h, followed by immunoblotting for Atg5, a marker for autophagosomes, using Jess (ProteinSimple, Inc.) (J) Total protein per capillary was used as loading control to normalize the levels of Atg5. Mn did not further increase the accumulation of autophagosomes when combined with CQ versus CQ alone (P = 0.9998). All data represent mean +- SEM (n = 3-4 independent experiments with approximately 30 cells per experiment for E and G) and groups were compared by using Kruskal-Wallis ANOVA test with Dunn's post-hoc test; ns: non-significant. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-024-00708-w ), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nb110-53818_rabbit-polyclonal-atg5-antibody-30820241044550.jpg" class="big_thumb" alt="Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P 0.9999), as compared to CQ (50 uM) only, a dose that did not completely inhibit autophagy. Data represent mean +- SEM. (H) N27 cells were treated with 62.5 or 125 uM of MnCl2 for 24h, then incubated with LysoSensor Yellow/Blue. The ratio of acidic (yellow) vs. neutral (blue) pH of lysosomes were quantified using a plate reader. (I) N27 cells were treated with Mn 125 uM, CQ50 uM or both for 24h, followed by immunoblotting for Atg5, a marker for autophagosomes, using Jess (ProteinSimple, Inc.) (J) Total protein per capillary was used as loading control to normalize the levels of Atg5. Mn did not further increase the accumulation of autophagosomes when combined with CQ versus CQ alone (P = 0.9998). All data represent mean +- SEM (n = 3-4 independent experiments with approximately 30 cells per experiment for E and G) and groups were compared by using Kruskal-Wallis ANOVA test with Dunn's post-hoc test; ns: non-significant. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-024-00708-w ), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P 0.9999), as compared to CQ (50 uM) only, a dose that did not completely inhibit autophagy. Data represent mean +- SEM. (H) N27 cells were treated with 62.5 or 125 uM of MnCl2 for 24h, then incubated with LysoSensor Yellow/Blue. The ratio of acidic (yellow) vs. neutral (blue) pH of lysosomes were quantified using a plate reader. (I) N27 cells were treated with Mn 125 uM, CQ50 uM or both for 24h, followed by immunoblotting for Atg5, a marker for autophagosomes, using Jess (ProteinSimple, Inc.) (J) Total protein per capillary was used as loading control to normalize the levels of Atg5. Mn did not further increase the accumulation of autophagosomes when combined with CQ versus CQ alone (P = 0.9998). All data represent mean +- SEM (n = 3-4 independent experiments with approximately 30 cells per experiment for E and G) and groups were compared by using Kruskal-Wallis ANOVA test with Dunn's post-hoc test; ns: non-significant. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-024-00708-w ), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nb110-53818_rabbit-polyclonal-atg5-antibody-31020241624916.jpg" class="top_images" rel="gallery" title="ATG5 & ATG7 enhanced autophagy & inhibited ER stress in chondrocyte. a Western blotting analysis of LC3, P62, ATG5, ATG7 & ATG5-ATG12 expression after infected with Ad-ATG5, Ad-ATG7 & Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta -actin is served as an internal control. b Qualitative analysis of ATG5, ATG7, ATG5-ATG12, LC3 & P62. The values were normalized to beta -actin. c C28I2 cells were double stained with LC3 (red) & DAPI (blue) & visualized by confocal microscopy (400X) after treated with Rapamycine, Ad-ATG5, Ad-ATG7 & Ad-ATG5 + Ad-ATG7 24 h. *P UAS‐Atg5‐RNAi flies (Atg5‐RNAi). Atg5 was reduced in Atg5‐RNAi flies (n = 6, one‐way ANOVA, *P < 0.05 vs. control). (D, E) Downregulation of autophagy abolished the beta ‐GPA‐mediated lifespan extension (n = 200, P < 0.0001 vs. control, log‐rank test). For all Western blots, relative band intensity was estimated using IMAGEJ. Data are shown as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26120775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nb110-53818_rabbit-polyclonal-atg5-antibody-31020241624916.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="ATG5 & ATG7 enhanced autophagy & inhibited ER stress in chondrocyte. a Western blotting analysis of LC3, P62, ATG5, ATG7 & ATG5-ATG12 expression after infected with Ad-ATG5, Ad-ATG7 & Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta -actin is served as an internal control. b Qualitative analysis of ATG5, ATG7, ATG5-ATG12, LC3 & P62. The values were normalized to beta -actin. c C28I2 cells were double stained with LC3 (red) & DAPI (blue) & visualized by confocal microscopy (400X) after treated with Rapamycine, Ad-ATG5, Ad-ATG7 & Ad-ATG5 + Ad-ATG7 24 h. *P UAS‐Atg5‐RNAi flies (Atg5‐RNAi). Atg5 was reduced in Atg5‐RNAi flies (n = 6, one‐way ANOVA, *P < 0.05 vs. control). (D, E) Downregulation of autophagy abolished the beta ‐GPA‐mediated lifespan extension (n = 200, P < 0.0001 vs. control, log‐rank test). For all Western blots, relative band intensity was estimated using IMAGEJ. Data are shown as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26120775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="ATG5 & ATG7 enhanced autophagy & inhibited ER stress in chondrocyte. a Western blotting analysis of LC3, P62, ATG5, ATG7 & ATG5-ATG12 expression after infected with Ad-ATG5, Ad-ATG7 & Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta -actin is served as an internal control. b Qualitative analysis of ATG5, ATG7, ATG5-ATG12, LC3 & P62. The values were normalized to beta -actin. c C28I2 cells were double stained with LC3 (red) & DAPI (blue) & visualized by confocal microscopy (400X) after treated with Rapamycine, Ad-ATG5, Ad-ATG7 & Ad-ATG5 + Ad-ATG7 24 h. *P UAS‐Atg5‐RNAi flies (Atg5‐RNAi). Atg5 was reduced in Atg5‐RNAi flies (n = 6, one‐way ANOVA, *P < 0.05 vs. control). (D, E) Downregulation of autophagy abolished the beta ‐GPA‐mediated lifespan extension (n = 200, P < 0.0001 vs. control, log‐rank test). For all Western blots, relative band intensity was estimated using IMAGEJ. Data are shown as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26120775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >ATG5 & ATG7 enhanced autophagy & inhibited ER stress in chondrocyte. a Western blotting analysis of LC3, P62, ATG5, ATG7 & ATG5-ATG12 expression after infected with Ad-ATG5, Ad-ATG7 & Ad-ATG5 + Ad-ATG7 in the C28I2<a class="openGallery" href="#" data-picnum="1"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nb110-53818_rabbit-polyclonal-atg5-antibody-3102024162495.jpg" class="top_images" rel="gallery" title="Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a fluorescent reporter plasmid to monitor autophagic flux at 8 h following the indicated treatments. GFP (green) & GFP + mRFP (yellow) fluorescence are observed prior to the fusion of autophagosomes with lysosomes whereas only mRFP (red) fluorescence is present in post-fusion autolysosomes. DIC, differential interference contrast microscopy image. DAPI (blue) staining indicates cell nuclei. (B) Quantification of autolysosomes (red puncta) from (A). F(3,13) = 8.756, p = 0.0019; ∗p < 0.05 when compared to all other groups. (C) Western blotting analysis of the indicated autophagy associated protein levels at 24 h following the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Quantification of dendrite beading from (A). F(3,77) = 6.429, p = 0.0006; ∗p < 0.05 when compared to control cells. Error bars show the SEM. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nb110-53818_rabbit-polyclonal-atg5-antibody-3102024162495.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a fluorescent reporter plasmid to monitor autophagic flux at 8 h following the indicated treatments. GFP (green) & GFP + mRFP (yellow) fluorescence are observed prior to the fusion of autophagosomes with lysosomes whereas only mRFP (red) fluorescence is present in post-fusion autolysosomes. DIC, differential interference contrast microscopy image. DAPI (blue) staining indicates cell nuclei. (B) Quantification of autolysosomes (red puncta) from (A). F(3,13) = 8.756, p = 0.0019; ∗p < 0.05 when compared to all other groups. (C) Western blotting analysis of the indicated autophagy associated protein levels at 24 h following the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Quantification of dendrite beading from (A). F(3,77) = 6.429, p = 0.0006; ∗p < 0.05 when compared to control cells. Error bars show the SEM. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a fluorescent reporter plasmid to monitor autophagic flux at 8 h following the indicated treatments. GFP (green) & GFP + mRFP (yellow) fluorescence are observed prior to the fusion of autophagosomes with lysosomes whereas only mRFP (red) fluorescence is present in post-fusion autolysosomes. DIC, differential interference contrast microscopy image. DAPI (blue) staining indicates cell nuclei. (B) Quantification of autolysosomes (red puncta) from (A). F(3,13) = 8.756, p = 0.0019; ∗p < 0.05 when compared to all other groups. (C) Western blotting analysis of the indicated autophagy associated protein levels at 24 h following the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Quantification of dendrite beading from (A). F(3,77) = 6.429, p = 0.0006; ∗p < 0.05 when compared to control cells. Error bars show the SEM. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a<a class="openGallery" href="#" data-picnum="2"> ...read more</a></div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span>, <span title='Mouse'>Mu</span>, <span title='Rat'>Rt</span>, <span title='Porcine'>Po</span>, <span title='Alligator'>Al</span>, <span title='Bovine'>Bv</span>, <span title='Drosophila'>Dr</span>, <span title='Fish'>Fi</span>, <span title='Guinea Pig'>Gp</span>, <span title='Primate'>Pm</span>, <span title='Xenopus'>Xp</span>, <span title='Zebrafish'>Ze</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='Simple Western'>Simple Western</span>, <span title='ELISA'>ELISA</span>, <span title='Flow Cytometry'>Flow</span>, <span title='Functional Assay'>Func</span>, <span title='Immunocytochemistry/ Immunofluorescence'>ICC/IF</span>, <span title='Immunohistochemistry'>IHC</span>, <span title='Immunoprecipitation'>IP</span>, <span title='Microscopy'>MS</span>, <span title='Western Blot'>WB</span>, <span title='Knockdown Validated'>KD</span>, <span title='Knockout Validated'>KO</span></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr><tr class="lined"> <td><strong>Format</strong></td> <td><div>BSA Free</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>1.0 mg/ml</div></td> </tr></table></div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="" data-catnum="NB110-53818" data-catnums="NB110-53818,NB110-53818SS" data-disabled=''><noscript><form autocomplete="off" action="https://www.google.com/" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size / Catalog#</th><th>Qty</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div data-msg='' class="atc_catnum">NB110-53818SS</div><div class="atc_size" title="catalog # NB110-53818SS">0.025 ml</div></td><td><div class="form-item form-type-textfield form-item-rows-NB110-53818SS-quantity"> <input type="text" id="edit-rows-nb110-53818ss-quantity" name="rows[NB110-53818SS][quantity]" value="" size="60" maxlength="2" class="form-text" /> </div> </td><td><div class="price"> $219.00</div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">8-10 Business Days</td> </tr> <tr class="odd"><td><div data-msg='' class="atc_catnum">NB110-53818</div><div class="atc_size" title="catalog # NB110-53818">0.1 ml</div></td><td><div class="form-item form-type-textfield form-item-rows-NB110-53818-quantity"> <input type="text" id="edit-rows-nb110-53818-quantity" name="rows[NB110-53818][quantity]" value="" size="60" maxlength="2" class="form-text" /> </div> </td><td><div class="price"> $449.00</div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">In Stock: 7-9 Business Days</td> </tr> </tbody> </table> <input class="bulk_custom iframeBulkQuote form-submit" onClick="window.location.assign(&quot;https://www.novusbio.com/novus_quoterequest?mlt=ATG5+Antibody+-+BSA+Free&amp;catnum=NB110-53818&quot;); return false;" type="submit" id="edit-bulk-request" name="op" value="Bulk Request" /><input class="add_to_cart form-submit" type="submit" id="edit-add-multiple-to-cart" name="op" 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class="conj_link">Alexa Fluor 488</a><br /> <a href="/products/atg5-antibody_nb110-53818af488" class="cat_link">NB110-53818AF488</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/389497"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF532"> <td> <a href="/products/atg5-antibody_nb110-53818af532" class="conj_link">Alexa Fluor 532</a><br /> <a href="/products/atg5-antibody_nb110-53818af532" class="cat_link">NB110-53818AF532</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/719248"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF594"> <td> <a href="/products/atg5-antibody_nb110-53818af594" class="conj_link">Alexa Fluor 594</a><br /> <a href="/products/atg5-antibody_nb110-53818af594" class="cat_link">NB110-53818AF594</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/707924"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF647"> <td> <a href="/products/atg5-antibody_nb110-53818af647" class="conj_link">Alexa Fluor 647</a><br /> <a href="/products/atg5-antibody_nb110-53818af647" class="cat_link">NB110-53818AF647</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/389498"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF700"> <td> <a href="/products/atg5-antibody_nb110-53818af700" class="conj_link">Alexa Fluor 700</a><br /> <a href="/products/atg5-antibody_nb110-53818af700" class="cat_link">NB110-53818AF700</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/389499"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF750"> <td> <a href="/products/atg5-antibody_nb110-53818af750" class="conj_link">Alexa Fluor 750</a><br /> <a href="/products/atg5-antibody_nb110-53818af750" class="cat_link">NB110-53818AF750</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/707925"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818APC"> <td> <a href="/products/atg5-antibody_nb110-53818apc" class="conj_link">Allophycocyanin</a><br /> <a href="/products/atg5-antibody_nb110-53818apc" class="cat_link">NB110-53818APC</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1040277"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818B"> <td> <a href="/products/atg5-antibody_nb110-53818b" class="conj_link">Biotin</a><br /> <a href="/products/atg5-antibody_nb110-53818b" class="cat_link">NB110-53818B</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/55821"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818CL1"> <td> <a href="/products/atg5-antibody_nb110-53818cl1" class="conj_link">CoraFluor 1</a><br /> <a href="/products/atg5-antibody_nb110-53818cl1" class="cat_link">NB110-53818CL1</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1310621"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818UV"> <td> <a href="/products/atg5-antibody_nb110-53818uv" class="conj_link">DyLight 350</a><br /> <a href="/products/atg5-antibody_nb110-53818uv" class="cat_link">NB110-53818UV</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/721501"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818V"> <td> <a href="/products/atg5-antibody_nb110-53818v" class="conj_link">DyLight 405</a><br /> <a href="/products/atg5-antibody_nb110-53818v" class="cat_link">NB110-53818V</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/335229"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818G"> <td> <a href="/products/atg5-antibody_nb110-53818g" class="conj_link">DyLight 488</a><br /> <a href="/products/atg5-antibody_nb110-53818g" class="cat_link">NB110-53818G</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/29777"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818R"> <td> <a href="/products/atg5-antibody_nb110-53818r" class="conj_link">DyLight 550</a><br /> <a href="/products/atg5-antibody_nb110-53818r" class="cat_link">NB110-53818R</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/94215"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818DL594"> <td> <a href="/products/atg5-antibody_nb110-53818dl594" class="conj_link">DyLight 594</a><br /> <a href="/products/atg5-antibody_nb110-53818dl594" class="cat_link">NB110-53818DL594</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/723598"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818C"> <td> <a href="/products/atg5-antibody_nb110-53818c" class="conj_link">DyLight 650</a><br /> <a href="/products/atg5-antibody_nb110-53818c" class="cat_link">NB110-53818C</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/4156"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818FR"> <td> <a href="/products/atg5-antibody_nb110-53818fr" class="conj_link">DyLight 680</a><br /> <a href="/products/atg5-antibody_nb110-53818fr" class="cat_link">NB110-53818FR</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1047472"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818IR"> <td> <a href="/products/atg5-antibody_nb110-53818ir" class="conj_link">DyLight 755</a><br /> <a href="/products/atg5-antibody_nb110-53818ir" class="cat_link">NB110-53818IR</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/729838"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818F"> <td> <a href="/products/atg5-antibody_nb110-53818f" class="conj_link">FITC</a><br /> <a href="/products/atg5-antibody_nb110-53818f" class="cat_link">NB110-53818F</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/334394"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818H"> <td> <a href="/products/atg5-antibody_nb110-53818h" class="conj_link">HRP</a><br /> <a href="/products/atg5-antibody_nb110-53818h" class="cat_link">NB110-53818H</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/62266"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818JF525"> <td> <a href="/products/atg5-antibody_nb110-53818jf525" class="conj_link">Janelia Fluor 525</a><br /> <a href="/products/atg5-antibody_nb110-53818jf525" class="cat_link">NB110-53818JF525</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1316322"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818JF585"> <td> <a href="/products/atg5-antibody_nb110-53818jf585" class="conj_link">Janelia Fluor 585</a><br /> <a href="/products/atg5-antibody_nb110-53818jf585" class="cat_link">NB110-53818JF585</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1316323"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818JF635"> <td> <a href="/products/atg5-antibody_nb110-53818jf635" class="conj_link">Janelia Fluor 635</a><br /> <a href="/products/atg5-antibody_nb110-53818jf635" class="cat_link">NB110-53818JF635</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1316324"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818JF669"> <td> <a href="/products/atg5-antibody_nb110-53818jf669" class="conj_link">Janelia Fluor 669</a><br /> <a href="/products/atg5-antibody_nb110-53818jf669" class="cat_link">NB110-53818JF669</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1316325"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818PE"> <td> <a href="/products/atg5-antibody_nb110-53818pe" class="conj_link">PE</a><br /> <a href="/products/atg5-antibody_nb110-53818pe" class="cat_link">NB110-53818PE</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1040278"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818PCP"> <td> <a href="/products/atg5-antibody_nb110-53818pcp" class="conj_link">PerCP</a><br /> <a href="/products/atg5-antibody_nb110-53818pcp" class="cat_link">NB110-53818PCP</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1040279"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818MFV450"> <td> <a href="/products/atg5-antibody_nb110-53818mfv450" class="conj_link">mFluor Violet 450 SE</a><br /> <a href="/products/atg5-antibody_nb110-53818mfv450" class="cat_link">NB110-53818MFV450</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1159404"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818MFV500"> <td> <a href="/products/atg5-antibody_nb110-53818mfv500" class="conj_link">mFluor Violet 500 SE</a><br /> <a href="/products/atg5-antibody_nb110-53818mfv500" class="cat_link">NB110-53818MFV500</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1159405"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818MFV610"> <td> <a href="/products/atg5-antibody_nb110-53818mfv610" class="conj_link">mFluor Violet 610 SE</a><br /> <a href="/products/atg5-antibody_nb110-53818mfv610" class="cat_link">NB110-53818MFV610</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/1159406"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr> <tr class="scf_footer"> <td colspan="4"><div class="expander option_opened"></div></td> </tr> </tbody> </table> <div class="popup_skus" data-skus='["NB110-53818AF350","NB110-53818AF405","NB110-53818AF488","NB110-53818AF532","NB110-53818AF594","NB110-53818AF647","NB110-53818AF700","NB110-53818AF750","NB110-53818APC","NB110-53818B","NB110-53818CL1","NB110-53818UV","NB110-53818V","NB110-53818G","NB110-53818R","NB110-53818DL594","NB110-53818C","NB110-53818FR","NB110-53818IR","NB110-53818F","NB110-53818H","NB110-53818JF525","NB110-53818JF585","NB110-53818JF635","NB110-53818JF669","NB110-53818PE","NB110-53818PCP","NB110-53818MFV450","NB110-53818MFV500","NB110-53818MFV610"]' data-rendered="false"></div></div><div class="list_expand options_closed" data-name="Formulations" data-wrapper-type=".formulations_wrapper"><div class="options_text">View Available Formulations</div> <div class="expander option_closed"></div></div><div class="formulations_wrapper" data-title="ATG5 Antibody - BSA Free" data-catnum="NB110-53818"><table class="scf_table"> <thead> <tr> <th>Catalog# &amp; Formulation</th> <th>Size</th> <th class="col_price">Price</th> <th class="col_cart"></th> </tr> </thead> <tbody><tr class="popup_sku" data-sku="NB110-53818SS"> <td> <a href="/products/atg5-antibody_nb110-53818" class="conj_link">BSA Free</a><br /> <a href="/products/atg5-antibody_nb110-53818" class="cat_link">NB110-53818SS</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart"><a href="/commerce/add-to-cart/69568"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr> <tr class="scf_footer"> <td colspan="4"><div class="expander option_opened"></div></td> </tr> </tbody> </table> <div class="popup_skus" data-skus='["NB110-53818SS"]' data-rendered="false"></div></div></div><div id="shipping_statement"><span id="shipping_rate"></span></div><div class="shipping_obt_transfer"><span>Novus Biologicals is part of Bio-Techne</span><br><br><a href="https://www.bio-techne.com/p/antibodies/atg5-antibody_nb110-53818" rel="follow" class="btn btn-primary">Shop this product on bio-techne.com</a></div><br><div class="learn_more"> <a href="/guarantee"> <div class="sprite_noImage" title="Novus Biologicals, part of Bio-Techne, offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals, part of Bio-Techne, offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"><table class="scf_table"> <thead> <tr> <th>Conjugate</th> <th>Catalog #</th> <th class="head_avail">Availability</th> <th>Size</th> <th class="col_price">Price</th> </tr> </thead> <tbody><tr class="popup_sku" data-sku="NB110-53818AF350"> <td><a href="/products/atg5-antibody_nb110-53818af350">Alexa Fluor 350</a></td> <td><a href="/products/atg5-antibody_nb110-53818af350">NB110-53818AF350</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF405"> <td><a href="/products/atg5-antibody_nb110-53818af405">Alexa Fluor 405</a></td> <td><a href="/products/atg5-antibody_nb110-53818af405">NB110-53818AF405</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF488"> <td><a href="/products/atg5-antibody_nb110-53818af488">Alexa Fluor 488</a></td> <td><a href="/products/atg5-antibody_nb110-53818af488">NB110-53818AF488</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF532"> <td><a href="/products/atg5-antibody_nb110-53818af532">Alexa Fluor 532</a></td> <td><a href="/products/atg5-antibody_nb110-53818af532">NB110-53818AF532</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF594"> <td><a href="/products/atg5-antibody_nb110-53818af594">Alexa Fluor 594</a></td> <td><a href="/products/atg5-antibody_nb110-53818af594">NB110-53818AF594</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF647"> <td><a href="/products/atg5-antibody_nb110-53818af647">Alexa Fluor 647</a></td> <td><a href="/products/atg5-antibody_nb110-53818af647">NB110-53818AF647</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF700"> <td><a href="/products/atg5-antibody_nb110-53818af700">Alexa Fluor 700</a></td> <td><a href="/products/atg5-antibody_nb110-53818af700">NB110-53818AF700</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818AF750"> <td><a href="/products/atg5-antibody_nb110-53818af750">Alexa Fluor 750</a></td> <td><a href="/products/atg5-antibody_nb110-53818af750">NB110-53818AF750</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818APC"> <td><a href="/products/atg5-antibody_nb110-53818apc">Allophycocyanin</a></td> <td><a href="/products/atg5-antibody_nb110-53818apc">NB110-53818APC</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> 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class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818IR"> <td><a href="/products/atg5-antibody_nb110-53818ir">DyLight 755</a></td> <td><a href="/products/atg5-antibody_nb110-53818ir">NB110-53818IR</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818F"> <td><a href="/products/atg5-antibody_nb110-53818f">FITC</a></td> <td><a href="/products/atg5-antibody_nb110-53818f">NB110-53818F</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818H"> <td><a href="/products/atg5-antibody_nb110-53818h">HRP</a></td> <td><a href="/products/atg5-antibody_nb110-53818h">NB110-53818H</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818JF525"> <td><a href="/products/atg5-antibody_nb110-53818jf525">Janelia Fluor 525</a></td> <td><a href="/products/atg5-antibody_nb110-53818jf525">NB110-53818JF525</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818JF585"> <td><a href="/products/atg5-antibody_nb110-53818jf585">Janelia Fluor 585</a></td> <td><a href="/products/atg5-antibody_nb110-53818jf585">NB110-53818JF585</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818JF635"> <td><a href="/products/atg5-antibody_nb110-53818jf635">Janelia Fluor 635</a></td> <td><a 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href="/products/atg5-antibody_nb110-53818pcp">PerCP</a></td> <td><a href="/products/atg5-antibody_nb110-53818pcp">NB110-53818PCP</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818MFV450"> <td><a href="/products/atg5-antibody_nb110-53818mfv450">mFluor Violet 450 SE</a></td> <td><a href="/products/atg5-antibody_nb110-53818mfv450">NB110-53818MFV450</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818MFV500"> <td><a href="/products/atg5-antibody_nb110-53818mfv500">mFluor Violet 500 SE</a></td> <td><a href="/products/atg5-antibody_nb110-53818mfv500">NB110-53818MFV500</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr><tr class="popup_sku" data-sku="NB110-53818MFV610"> <td><a href="/products/atg5-antibody_nb110-53818mfv610">mFluor Violet 610 SE</a></td> <td><a href="/products/atg5-antibody_nb110-53818mfv610">NB110-53818MFV610</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr> <tr class="scf_footer"> <td colspan="5"><div class="expander option_opened"></div></td> </tr> </tbody> </table> <div class="popup_skus" data-skus='["NB110-53818AF350","NB110-53818AF405","NB110-53818AF488","NB110-53818AF532","NB110-53818AF594","NB110-53818AF647","NB110-53818AF700","NB110-53818AF750","NB110-53818APC","NB110-53818B","NB110-53818CL1","NB110-53818UV","NB110-53818V","NB110-53818G","NB110-53818R","NB110-53818DL594","NB110-53818C","NB110-53818FR","NB110-53818IR","NB110-53818F","NB110-53818H","NB110-53818JF525","NB110-53818JF585","NB110-53818JF635","NB110-53818JF669","NB110-53818PE","NB110-53818PCP","NB110-53818MFV450","NB110-53818MFV500","NB110-53818MFV610"]' data-rendered="false"></div></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews &amp; Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols &amp; FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support &amp; Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>ATG5 Antibody - BSA Free Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div>This ATG5 Antibody was made to a synthetic peptide of an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0].</div></td> </tr><tr class="lined"> <td><strong>Localization</strong></td> <td><div>Cytoplasm. Co-localizes with non-muscle actin.</div></td> </tr><tr class="lined"> <td><strong>Specificity</strong></td> <td><div>This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275.</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>ATG5</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Immunogen affinity purified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Electron Microscopy </li><li>ELISA </li><li>Flow Cytometry </li><li>Immunoblotting </li><li>Immunocytochemistry/ Immunofluorescence 1:250</li><li>Immunohistochemistry 1:400</li><li>Immunohistochemistry-Paraffin 1:400</li><li>Immunoprecipitation 1:10 - 1:500</li><li>Knockdown Validated </li><li>Knockout Validated </li><li>Proximity Ligation Assay </li><li>Radioimmunoassay </li><li>Simple Western 1:50</li><li>Western Blot 1:500</li> </ul> </td> </tr><tr class="lined"> <td><strong>Theoretical MW</strong></td> <td><div>32 kDa.<br> Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.</div></td> </tr> <tr class="lined"> <td><strong>Control</strong></td> <td> <table class="wide ds_altTable"><tr> <td style="width:80%"><a href="/atg5-lysate_nbl1-07799">ATG5 Overexpression Lysate</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NBL1-07799" data-return="atg5-antibody_nb110-53818" data-produrl="atg5-antibody_nb110-53818" data-catnums="NBL1-07799" ></div> </td> </tr></table> </td> </tr> <tr class="lined"> <td><strong>Control Peptide</strong></td> <td> <table class="wide ds_altTable"><tr> <td style="width:80%"><a href="/atg5-protein_nb110-53818pep">ATG5 Protein (NB110-53818PEP)</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NB110-53818PEP" data-return="atg5-antibody_nb110-53818" data-produrl="atg5-antibody_nb110-53818" data-catnums="NB110-53818PEP" ></div> </td> </tr></table> </td> </tr><tr class="lined"> <td><strong>Reviewed Applications</strong></td> <td> <table class="wide"> <tr> <td> Read <a href="#ReviewsSection">8 Reviews</a> rated 4.3<div class="dsSprite_stars4"></div> using <br/>NB110-53818 in the following applications:<br /><br /> <ul class="information-list"><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Western Blot">Western Blot</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Immunohistochemistry-Paraffin">Immunohistochemistry-Paraffin</a></div> <div class="information-data"></div> </li> </ul> </td> </tr> </table> </td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> NB110-53818 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Block/Neutralize">Block/Neutralize</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="ELISA">ELISA</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="EM">EM</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="FLOW">FLOW</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="ICC/IF">ICC/IF</a> </div><div class="information-data">11 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IF/IHC">IF/IHC</a> </div><div class="information-data">12 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-Fr">IHC-Fr</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-P">IHC-P</a> </div><div class="information-data">9 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IP">IP</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Immunocytochemistry/ Immunofluorescence">Immunocytochemistry/ Immunofluorescence</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Immunohistochemistry">Immunohistochemistry</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="In vivo assay">In vivo assay</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="KO">KO</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="PLA">PLA</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="RIA">RIA</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="WB">WB</a> </div><div class="information-data">164 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Western Blot">Western Blot</a> </div><div class="information-data">6 publications</div></li></ul></td> </tr> </table> </td> </tr> </table> </div><h2 class="greyHead2">Reactivity Notes</h2> <div> <a id="tab1speciesreactivity"></a> <div class="ds_info"> Drosophila reactivity reported in scientific literature (PMID:33221768) Fish reactivity reported in scientific literature (PMID: 26183773). Guinea Pig reactivity reported in scientific literature (PMID: 30766882). Use in Alligator reported in scientific literature (PMID:32061056).</div> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div>Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.</div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>PBS</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>0.02% Sodium Azide</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>1.0 mg/ml</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Immunogen affinity purified</div></td> </tr></table></div><h2 class="greyHead2">Alternate Names for ATG5 Antibody - BSA Free</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>APG5 (Autophagy 5, S. Cerevisiae)-Like</li><li>APG5 autophagy 5-like (S. cerevisiae)</li><li>APG5</li><li>APG5L</li><li>APG5-LIKE</li><li>ASP</li><li>ASPAPG5-LIKE</li><li>ATG5 autophagy related 5 homolog (S. cerevisiae)</li><li>ATG5 Autophagy Related 5 Homolog</li><li>ATG5</li><li>Autophagy protein 5</li><li>Autophagy Related 5</li><li>HAPG5</li><li>SCAR25</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div><a target="_blank" href="https://www.novusbio.com/common-name/atg5">Atg5</a>, Autophagy related 5 or autophagy protein 5 (theoretical molecular weight 32 kDa), belongs to a group of core <a target="_blank" href="https://www.novusbio.com/research-areas/autophagy">autophagy</a>-related proteins first identified in yeast and later in eukaryotic cells. Atg proteins play essential roles in the process of macroautophagy. Atg5 is considered a core autophagy protein, for its role in the formation of the autophagosome, a double membrane vesicle which engulfs proteins and organelles for delivery to the <a target="_blank" href="https://www.novusbio.com/research-areas/cellular-markers/lysosome-markers">lysosome</a> and subsequent degradation (1). Atg5 participates in the process of phagophore elongation by interacting with the ubiquitin-like protein <a target="_blank" href="https://www.novusbio.com/common-name/atg12">Atg12</a>. Formation of the Atg12-Atg5 conjugate is dependent on the activities of <a target="_blank" href="https://www.novusbio.com/common-name/atg7">Atg7</a> (E1 ubiquitin-activating like enzyme) and <a target="_blank" href="https://www.novusbio.com/common-name/atg10">Atg10</a> (E2 ubiquitin-activating like enzyme). Non-covalent interaction between the Atg12-Atg5 conjugate and <a target="_blank" href="https://www.novusbio.com/common-name/atg16l1">Atg16L1</a>, allows for the formation of a large complex which associates with the nascent phagophore. The Atg16L1 complex dissociates from the autophagosome once it is fully formed (1,2). <br/><br/> In the context of its role in autophagy, Atg5 plays diverse physiologically relevant roles. For example, Atg5 together with Atg7 are required for adipogenesis (3). Recently, Atg5 has been implicated in the process of B-cell receptor polarization and antigen presentation (4). In addition to its role in autophagy, Atg5 is implicated in <a target="_blank" href="https://www.novusbio.com/research-areas/apoptosis">apoptotic cell death</a>. Interaction of Atg5 with <a target="_blank" href="https://www.novusbio.com/common-name/fadd">FADD</a> (Fas-associated protein with death domain) is involved in cell death induced by <a target="_blank" href="https://www.novusbio.com/common-name/ifn-gamma">IFN-gamma</a>. A truncated form of Atg5, a 24kDa fragment, leads to cell death by interacting with <a target="_blank" href="https://www.novusbio.com/common-name/bcl-xl">Bcl-xl</a> and inhibiting its anti-apoptotic activity (5). Other Atg5 interacting partners include <a target="_blank" href="https://www.novusbio.com/common-name/caspase-1">interleukin-beta (IL-beta) converting enzyme</a> and <a target="_blank" href="https://www.novusbio.com/common-name/nod1">nucleotide binding oligomerization domain protein 1</a>, which suggest that Atg5 may play other biologically relevant roles (3). <br/><br/> References <br/><br/> 1. Yang, Z., & Klionsky, D. J. (2010). Mammalian autophagy: Core molecular machinery and signaling regulation. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2009.11.014 <br/><br/> 2. Rubinsztein, D. C., Shpilka, T., & Elazar, Z. (2012). Mechanisms of autophagosome biogenesis. Current Biology. https://doi.org/10.1016/j.cub.2011.11.034 <br/><br/> 3. Subramani, S., & Malhotra, V. (2013). Non-autophagic roles of autophagy-related proteins. EMBO Reports. https://doi.org/10.1038/embor.2012.220 <br/><br/> 4. Arbogast, F., Arnold, J., Hammann, P., Kuhn, L., Chicher, J., Murera, D., Gros, F. (2019). ATG5 is required for B cell polarization and presentation of particulate antigens. Autophagy. https://doi.org/10.1080/15548627.2018.1516327 <br/><br/> 5. Luo, S., & Rubinsztein, D. C. (2007). Atg5 and Bcl-2 provide novel insights into the interplay between apoptosis and autophagy. Cell Death and Differentiation. https://doi.org/10.1038/sj.cdd.4402149</div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are <a href="/support/quality-assurance.html">guaranteed</a> for 1 year from date of receipt.</div> </div><h2 class="greyHead2">Customers Who Viewed This Item Also Viewed...</h2> <a id="custalsoviewed"></a><div id="cav_prev"><div class="sprite_leftArrow"></div></div> <div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout=0 data-cycle-prev="#cav_prev" data-cycle-next="#cav_next" data-cycle-slides="div.ds_cav_group" id="ds_customer_also_viewed"><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB500-249"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/beclin-1-antibody_nb500-249" style="overflow: hidden; width: 280px;" title="Beclin 1 Antibody - BSA Free">Beclin 1 Antibody<br> - BSA Free</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NB500-249" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NB500-249" data-baseurl="https://www.novusbio.com" data-productid="36911"></div>--> <!-- <a href="/commerce/add-to-cart/36911"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NB500-249</div> <div class="images_customers"><div id="ss_prev_cav0" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev_cav0" data-cycle-next="#ss_next_cav0" data-cycle-slides="div.big_thumb_cust_container" data-index=0 ><div class="big_thumb_cust_container"> <a href="/products/beclin-1-antibody_nb500-249" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Representative Western blot images and quantitative analyses of Beclin 1 from the left hemisphere of rat brains at different time points after SAH. 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Cells were stained using the NorthernLights&amp;amp;#8482; 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg" alt="Immunocytochemistry Insulin Antibody (182410) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/insulin-antibody-182410_mab1417" title="Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5&amp;amp;#160;&amp;amp;micro;g/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte&amp;amp;trade; HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg" alt="Immunohistochemistry Insulin Antibody (182410) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/insulin-antibody-182410_mab1417#PublicationSection"> <div class="sprite_pubBook"></div> (24 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Hu, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, ICC, IHC, ICFlow</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB200-540"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/complement-c3-antibody-11h9_nb200-540" style="overflow: hidden; width: 280px;" title="Complement C3 Antibody (11H9) - BSA Free">Complement C3<br> Antibody (11H9) - BSA ...</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NB200-540" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NB200-540" data-baseurl="https://www.novusbio.com" data-productid="138478"></div>--> <!-- <a href="/commerce/add-to-cart/138478"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NB200-540</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/complement-c3-antibody-11h9_nb200-540" title="Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. WB image submitted by a verified customer review." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Complement-C3-Antibody-11H9-Flow-Cytometry-NB200-540-img0006.jpg" alt="Flow Cytometry Complement C3 Antibody (11H9) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/complement-c3-antibody-11h9_nb200-540" title="Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Complement-C3-Antibody-11H9-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence Complement C3 Antibody (11H9) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/complement-c3-antibody-11h9_nb200-540" title="Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Complement-C3-Antibody-11H9-Immunohistochemistry-Paraffin-NB200-540-img0003.jpg" alt="Immunohistochemistry-Paraffin Complement C3 Antibody (11H9) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/complement-c3-antibody-11h9_nb200-540#ReviewsSection"> <div class="sprite_5Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/complement-c3-antibody-11h9_nb200-540#PublicationSection"> <div class="sprite_pubBook"></div> (31 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Ec, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB600-607"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tor-mtor-antibody_nb600-607" style="overflow: hidden; width: 280px;" title="TOR/mTOR [p Ser2448] Antibody">TOR/mTOR [p<br> Ser2448] Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NB600-607" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NB600-607" data-baseurl="https://www.novusbio.com" data-productid="126603"></div>--> <!-- <a href="/commerce/add-to-cart/126603"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NB600-607</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/tor-mtor-antibody_nb600-607" title="Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg" alt="Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tor-mtor-antibody_nb600-607" title="Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY license." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg" alt="Western Blot TOR/mTOR [p Ser2448] Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tor-mtor-antibody_nb600-607" title="TOR/mTOR [p Ser2448] Antibody [NB600-607]" class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg" alt="Data TOR/mTOR [p Ser2448] Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/tor-mtor-antibody_nb600-607#ReviewsSection"> <div class="sprite_4Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/tor-mtor-antibody_nb600-607#PublicationSection"> <div class="sprite_pubBook"></div> (9 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00008878-M01"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/p62-sqstm1-antibody-2c11_h00008878-m01" style="overflow: hidden; width: 280px;" title="p62/SQSTM1 Antibody (2C11)">p62/SQSTM1<br> Antibody (2C11)</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="H00008878-M01" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="H00008878-M01" data-baseurl="https://www.novusbio.com" data-productid="45101"></div>--> <!-- <a href="/commerce/add-to-cart/45101"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">H00008878-M01</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/p62-sqstm1-antibody-2c11_h00008878-m01" title="Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62 in SH-SY5Y cells using anti-p62/SQSTM1 antibody. Red - p62 puncta; Blue - nuclear DAPI; Green - Cytoskeleton. Image from verified customer review." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/p62-SQSTM1-Antibody-2C11-Immunocytochemistry-Immunofluorescence-H00008878-M01-img0010.jpg" alt="Immunocytochemistry/ Immunofluorescence p62/SQSTM1 Antibody (2C11)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/p62-sqstm1-antibody-2c11_h00008878-m01" title="Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Confirmation of loss of hepatic ATG7 protein and a phenotype of hepatic autophagy inactivation. Fourth ug of liver protein were loaded on 4-20% gradient Tris-Glycine gels and subsequently immunoblotted with antibodies against ATG7, p62 and LC3. Blots were visualized with G:BOX Chemi XRQ (SynGene). Representative immunoblots are shown for WT and KO mice. Full-length immunoblots are presented in Supplementary Figure S1. ATG7: autophagy-related protein 7; p62: sequestosome 1; LC3: microtubule-associated protein 1 light chain 3; WT: Mx1-Cre-Atg7F/F mouse; KO: Mx1-Cre+Atg7F/F mouse. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-14405-w), licensed under a CC-BY license." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/p62-SQSTM1-Antibody-2C11-Western-Blot-H00008878-M01-img0015.jpg" alt="Western Blot p62/SQSTM1 Antibody (2C11)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/p62-sqstm1-antibody-2c11_h00008878-m01" title="Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Disruption of the VANGL2-p62/SQSTM1 interaction in breast cancer cells. Soft agar colony formation of T47D cells overexpressing GFP, GFP-VANGL2 and GFP-p62/SQSTM1 (right). Protein expression was revealed with anti-p62/SQSTM1, anti-VANGL2 and anti-GFP antibodies by western blot analysis (right). In anti-GFP blot, the arrowhead indicates position of co-migrating GFP-VANGL2 and GFP-p62/SQSTM1 and the asterisk pinpoints GFP alone. Error bars represent mean+/-s.d. (n=3). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/ncomms10318), licensed under a CC-BY license." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/p62-SQSTM1-Antibody-2C11-Western-Blot-H00008878-M01-img0014.jpg" alt="Western Blot p62/SQSTM1 Antibody (2C11)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/p62-sqstm1-antibody-2c11_h00008878-m01#ReviewsSection"> <div class="sprite_5Stars"></div> (9 Reviews</a>) </div> <div class="tiny"> <a href="/products/p62-sqstm1-antibody-2c11_h00008878-m01#PublicationSection"> <div class="sprite_pubBook"></div> (412 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Ha, Hu, Mu, Rb, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB100-56098"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/bcl-2-antibody_nb100-56098" style="overflow: hidden; width: 280px;" title="Bcl-2 Antibody">Bcl-2 Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NB100-56098" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NB100-56098" data-baseurl="https://www.novusbio.com" data-productid="34669"></div>--> <!-- <a href="/commerce/add-to-cart/34669"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NB100-56098</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/bcl-2-antibody_nb100-56098" title="Western Blot: Bcl-2 Antibody [NB100-56098] - EA reduced MPP+-induced dopaminergic neuronal apoptosis by increasing BDNF (brain-derived neurotrophic factor) expression and further Akt phosphorylation in the rat substantia nigra. Eight days after MPP+ administration, our Western blot results (MAB7566) show that MPP+ treatment reduced tyrosine hydroxylase and Bcl-2 expression in the ipsilateral side of the rat substantia nigra (SN), but not in the contralateral side. EA stimulation (50 Hz) enhanced mature BDNF, tyrosine hydroxylase, and Bcl-2 expression in the MPP+-treated ipsilateral side. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/18/9/1846), licensed under a CC-BY license." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/Bcl-2-Antibody-Western-Blot-NB100-56098-img0012.jpg" alt="Western Blot Bcl-2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/bcl-2-antibody_nb100-56098" title="Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/Bcl-2-Antibody-Western-Blot-NB100-56098-img0004.jpg" alt="Western Blot Bcl-2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/bcl-2-antibody_nb100-56098" title="Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 using this antibody at 1:2000, 20 ug/protein was loaded per lane. (1) Human Jurkat T cells. (2) Human RS11 lymphoma cells. (3-6) Human breast cancer cases. Patient sample in lane 5 lacked detectible Bcl-2 expression." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/Bcl-2-Antibody-Western-Blot-NB100-56098-img0005.jpg" alt="Western Blot Bcl-2 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/bcl-2-antibody_nb100-56098#ReviewsSection"> <div class="sprite_5Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/bcl-2-antibody_nb100-56098#PublicationSection"> <div class="sprite_pubBook"></div> (26 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Ca, Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB100-1533"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/pomc-antibody_nb100-1533" style="overflow: hidden; width: 280px;" title="POMC Antibody">POMC Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NB100-1533" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NB100-1533" data-baseurl="https://www.novusbio.com" data-productid="38436"></div>--> <!-- <a href="/commerce/add-to-cart/38436"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NB100-1533</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/pomc-antibody_nb100-1533" title="Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg" alt="Flow Cytometry POMC Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pomc-antibody_nb100-1533" title="Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/POMC-Antibody-Immunohistochemistry-NB100-1533-img0007.jpg" alt="Immunohistochemistry POMC Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pomc-antibody_nb100-1533" title="Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/POMC-Antibody-Immunohistochemistry-NB100-1533-img0008.jpg" alt="Immunohistochemistry POMC Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/pomc-antibody_nb100-1533#PublicationSection"> <div class="sprite_pubBook"></div> (10 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Po, Rt, Sh</div><div class="tiny"><strong>Applications:</strong> Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF835"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/caspase-3-antibody_af835" style="overflow: hidden; width: 280px;" title="Caspase-3 Antibody [Unconjugated] - Active">Caspase-3 Antibody<br> [Unconjugated] - Acti ...</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="AF835" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="AF835" data-baseurl="https://www.novusbio.com" data-productid="418523"></div>--> <!-- <a href="/commerce/add-to-cart/418523"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">AF835</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/caspase-3-antibody_af835" title="Caspase&amp;amp;#x2011;3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 &amp;amp;micro;g/mL Human/Mouse Active Caspase&amp;amp;#x2011;3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg" alt="Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/caspase-3-antibody_af835" title=" Caspase‑3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 1&amp;amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte&amp;amp;#8482; HRP Polymer Antibody (Catalog&amp;amp;nbsp;# VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg" alt="Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/caspase-3-antibody_af835" title="Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;amp;#8482; 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg" alt="Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/caspase-3-antibody_af835#ReviewsSection"> <div class="sprite_5Stars"></div> (7 Reviews</a>) </div> <div class="tiny"> <a href="/products/caspase-3-antibody_af835#PublicationSection"> <div class="sprite_pubBook"></div> (388 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ICC, IHC</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00002965-M01"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/gtf2h1-antibody-1f12-1b5_h00002965-m01" style="overflow: hidden; width: 280px;" title="GTF2H1 Antibody (1F12-1B5)">GTF2H1 Antibody<br> (1F12-1B5)</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="H00002965-M01" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="H00002965-M01" data-baseurl="https://www.novusbio.com" data-productid="116374"></div>--> <!-- <a href="/commerce/add-to-cart/116374"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">H00002965-M01</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/gtf2h1-antibody-1f12-1b5_h00002965-m01" title="Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0016.jpg" alt="Western Blot GTF2H1 Antibody (1F12-1B5)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gtf2h1-antibody-1f12-1b5_h00002965-m01" title="Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/GTF2H1-Antibody-1F12-1B5-Immunocytochemistry-Immunofluorescence-H00002965-M01-img0009.jpg" alt="Immunocytochemistry/ Immunofluorescence GTF2H1 Antibody (1F12-1B5)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gtf2h1-antibody-1f12-1b5_h00002965-m01" title="Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/GTF2H1-Antibody-1F12-1B5-Immunohistochemistry-Paraffin-H00002965-M01-img0011.jpg" alt="Immunohistochemistry-Paraffin GTF2H1 Antibody (1F12-1B5)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/gtf2h1-antibody-1f12-1b5_h00002965-m01#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IHC, IHC-P, KD, S-ELISA, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="210-TA"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" style="overflow: hidden; width: 280px;" title="TNF-alpha [Unconjugated]">TNF-alpha<br> [Unconjugated]</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="210-TA" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="210-TA" data-baseurl="https://www.novusbio.com" data-productid="447011"></div>--> <!-- <a href="/commerce/add-to-cart/447011"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">210-TA</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg" alt="SDS-PAGE TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg" alt="Bioactivity TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation)." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg" alt="SEC-MALS TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#ReviewsSection"> <div class="sprite_5Stars"></div> (3 Reviews</a>) </div> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#PublicationSection"> <div class="sprite_pubBook"></div> (811 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-31381"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/nup62-antibody_nbp1-31381" style="overflow: hidden; width: 280px;" title="NUP62 Antibody">NUP62 Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP1-31381" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NBP1-31381" data-baseurl="https://www.novusbio.com" data-productid="178336"></div>--> <!-- <a href="/commerce/add-to-cart/178336"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP1-31381</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/nup62-antibody_nbp1-31381" title="Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/NUP62-Antibody-Western-Blot-NBP1-31381-img0015.jpg" alt="Western Blot NUP62 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/nup62-antibody_nbp1-31381" title="Immunocytochemistry/Immunofluorescence: Nucleoporin p62 Antibody [NBP1-31381] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 um." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/Nucleoporin-p62-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31381-img0012.jpg" alt="Immunocytochemistry/ Immunofluorescence NUP62 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/nup62-antibody_nbp1-31381" title="Western Blot: Nucleoporin p62 Antibody [NBP1-31381] - PC-12 whole cell lysate (30 ug) stained wtih Nucleoporin p62 Antibody diluted at 1:5000." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/Nucleoporin-p62-Antibody-Western-Blot-NBP1-31381-img0002.jpg" alt="Western Blot NUP62 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/nup62-antibody_nbp1-31381#PublicationSection"> <div class="sprite_pubBook"></div> (4 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, IP, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-37399"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/dynactin-4-antibody-3g9d7_nbp2-37399" style="overflow: hidden; width: 280px;" title="dynactin 4 Antibody (3G9D7) - BSA Free">dynactin 4<br> Antibody (3G9D7) - BS</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP2-37399" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NBP2-37399" data-baseurl="https://www.novusbio.com" data-productid="424879"></div>--> <!-- <a href="/commerce/add-to-cart/424879"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP2-37399</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/dynactin-4-antibody-3g9d7_nbp2-37399" title="ELISA: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);" class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/dynactin-4-Antibody-3G9D7-ELISA-NBP2-37399-img0001.jpg" alt="ELISA dynactin 4 Antibody (3G9D7) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/dynactin-4-antibody-3g9d7_nbp2-37399" title="Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mAb against human DCTN4 recombinant protein. (Expected MW is 53.2 kDa)" class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/dynactin-4-Antibody-3G9D7-Western-Blot-NBP2-37399-img0002.jpg" alt="Western Blot dynactin 4 Antibody (3G9D7) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/dynactin-4-antibody-3g9d7_nbp2-37399" title="Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mAb against HEK293 (1) and DCTN4 (AA: ***)-hIgGFc transfected HEK293 (2) cell lysate." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/dynactin-4-Antibody-3G9D7-Western-Blot-NBP2-37399-img0003.jpg" alt="Western Blot dynactin 4 Antibody (3G9D7) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-02627"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/imp2-igf2bp2-antibody-oti3f9_nbp2-02627" style="overflow: hidden; width: 280px;" title="IMP2/IGF2BP2 Antibody (OTI3F9)">IMP2/IGF2BP2<br> Antibody (OTI3F9)</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP2-02627" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NBP2-02627" data-baseurl="https://www.novusbio.com" data-productid="314739"></div>--> <!-- <a href="/commerce/add-to-cart/314739"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP2-02627</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/imp2-igf2bp2-antibody-oti3f9_nbp2-02627" title="Flow Cytometry: IGF2BP2 Antibody (3F9) [NBP2-02627] - Analysis of Jurkat cells, using anti-IGF2BP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue)." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/IGF2BP2-Antibody-3F9-Flow-Cytometry-NBP2-02627-img0001.jpg" alt="Flow Cytometry IMP2/IGF2BP2 Antibody (OTI3F9)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/imp2-igf2bp2-antibody-oti3f9_nbp2-02627" title="Flow Cytometry: IGF2BP2 Antibody (3F9) [NBP2-02627] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody, and then analyzed by flow cytometry." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/IGF2BP2-Antibody-3F9-Flow-Cytometry-NBP2-02627-img0002.jpg" alt="Flow Cytometry IMP2/IGF2BP2 Antibody (OTI3F9)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/imp2-igf2bp2-antibody-oti3f9_nbp2-02627" title="Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/IGF2BP2-Antibody-3F9-Western-Blot-NBP2-02627-img0003.jpg" alt="Western Blot IMP2/IGF2BP2 Antibody (OTI3F9)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Ca, Hu, Pm, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-19151"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/sam68-antibody_nbp1-19151" style="overflow: hidden; width: 280px;" title="SAM68 Antibody">SAM68 Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP1-19151" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NBP1-19151" data-baseurl="https://www.novusbio.com" data-productid="91963"></div>--> <!-- <a href="/commerce/add-to-cart/91963"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP1-19151</div> <div class="images_customers"><div id="ss_prev_cav16" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/sam68-antibody_nbp1-19151" title="Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAM68 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB" class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/SAM68-Antibody-Immunohistochemistry-NBP1-19151-img0014.jpg" alt="Immunohistochemistry-Paraffin SAM68 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sam68-antibody_nbp1-19151" title="Western Blot: SAM68 Antibody [NBP1-19151] - Whole cell lysate from HeLa cells. SAM68 was also immunoprecipitated by rabbit anti-SAM68 antibodies NBP1-19152 and NBP1-19153." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/SAM68-Antibody-Western-Blot-NBP1-19151-img0010.jpg" alt="Western Blot SAM68 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav16" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-16" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/sam68-antibody_nbp1-19151#ReviewsSection"> <div class="sprite_3Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/sam68-antibody_nbp1-19151#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Hu, Ma</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-82090"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/gate-16-gabarapl2-antibody_nbp2-82090" style="overflow: hidden; width: 280px;" title="GATE-16/GABARAPL2 Antibody - BSA Free">GATE-16/GABARAPL2<br> Antibody - BSA Free</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP2-82090" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NBP2-82090" data-baseurl="https://www.novusbio.com" data-productid="756276"></div>--> <!-- <a href="/commerce/add-to-cart/756276"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP2-82090</div> <div class="images_customers"><div id="ss_prev_cav17" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/gate-16-gabarapl2-antibody_nbp2-82090" title="Western Blot: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Analysis of GATE-16/GABARAPL2 in human brain tissue lysate with GATE-16/GABARAPL2 antibody at (A) 1 and (B) 2 ug/ml." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/GATE-16-GABARAPL2-Antibody-Western-Blot-NBP2-82090-img0001.jpg" alt="Western Blot GATE-16/GABARAPL2 Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gate-16-gabarapl2-antibody_nbp2-82090" title="Immunohistochemistry-Paraffin: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Rat brain tissue with GATE-16/GABARAPL2 antibody at 5 ug/mL." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/GATE-16-GABARAPL2-Antibody-Immunohistochemistry-NBP2-82090-img0002.jpg" alt="Immunohistochemistry-Paraffin GATE-16/GABARAPL2 Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gate-16-gabarapl2-antibody_nbp2-82090" title="Immunocytochemistry/Immunofluorescence: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Immunofluorescence of GATE-16/GABARAPL2 in rat brain tissue with GATE-16/GABARAPL2 antibody at 20 ug/mL." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/GATE-16-GABARAPL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82090-img0003.jpg" alt="Immunocytochemistry/ Immunofluorescence GATE-16/GABARAPL2 Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav17" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-17" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-55202"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/gabarapl1-antibody_nbp1-55202" style="overflow: hidden; width: 280px;" title="GABARAPL1 Antibody">GABARAPL1 Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP1-55202" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NBP1-55202" data-baseurl="https://www.novusbio.com" data-productid="224288"></div>--> <!-- <a href="/commerce/add-to-cart/224288"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP1-55202</div> <div class="images_customers"><div id="ss_prev_cav18" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-18" data-cycle-prev="#ss_prev_cav18" data-cycle-next="#ss_next_cav18" data-cycle-slides="div.big_thumb_cust_container" data-index=18 ><div class="big_thumb_cust_container"> <a href="/products/gabarapl1-antibody_nbp1-55202" title="Immunohistochemistry-Paraffin: GABARAPL1 Antibody [NBP1-55202] - Human Brain lysate tissue at an Antibody concentration of 5.0 ug/ml using anti-GABARAPL1 Antibody (NBP1-55202)." class="cust_viewed_18" > <img loading="lazy" src="https://images.novusbio.com/images/GABARAPL1-Antibody-Immunohistochemistry-Paraffin-NBP1-55202-img0006.jpg" alt="Immunohistochemistry-Paraffin GABARAPL1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gabarapl1-antibody_nbp1-55202" title="Western Blot: GABARAPL1 Antibody [NBP1-55202] - Hela Whole Cell lysates, Antibody Dilution: 1.0 ug/ml." class="cust_viewed_18" > <img loading="lazy" src="https://images.novusbio.com/images/GABARAPL1-Antibody-Western-Blot-NBP1-55202-img0005.jpg" alt="Western Blot GABARAPL1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gabarapl1-antibody_nbp1-55202" title="Western Blot: GABARAPL1 Antibody [NBP1-55202] - Transfected 293T tissue lysate at a concentration of 1ug/ml." class="cust_viewed_18" > <img loading="lazy" src="https://images.novusbio.com/images/GABARAPL1-Antibody-Western-Blot-NBP1-55202-img0001.jpg" alt="Western Blot GABARAPL1 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav18" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-18" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB110-53818"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/atg5-antibody_nb110-53818" style="overflow: hidden; width: 280px;" title="ATG5 Antibody - BSA Free">ATG5 Antibody -<br> BSA Free</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NB110-53818" data-return="atg5-antibody_nb110-53818#datasheet" data-produrl="atg5-antibody_nb110-53818" data-catnums="NB110-53818" data-baseurl="https://www.novusbio.com" data-productid="27857"></div>--> <!-- <a href="/commerce/add-to-cart/27857"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NB110-53818</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-19" data-cycle-prev="#ss_prev_cav19" data-cycle-next="#ss_next_cav19" data-cycle-slides="div.big_thumb_cust_container" data-index=19 ><div class="big_thumb_cust_container"> <a href="/products/atg5-antibody_nb110-53818" title="ATG5 Antibody - BSA Free" class="cust_viewed_19"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="ATG5 Antibody - BSA Free" title="ATG5 Antibody - BSA Free" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt, Po, Al, Bv, Dr, Fi, Gp, Pm, Xp, Ze</div><div class="tiny"><strong>Applications:</strong> WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP, MS, WB, KD, KO</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for ATG5 Antibody (NB110-53818)(285)</h2><div class="ds_info"> <div>We have publications tested in 11 confirmed species: Human, Mouse, Rat, Alligator, Bovine, Drosophila, Drosophilia, Guinea Pig, Porcine, Primate, Zebrafish.<br /><br />We have publications tested in 17 applications: Block/Neutralize, ELISA, EM, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, In vivo assay, KO, PLA, RIA, WB, Western Blot.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Block/Neutralize"> <div style="display:inline;">Block/Neutralize</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="ELISA"> <div style="display:inline;">ELISA</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="EM"> <div style="display:inline;">EM</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="FLOW"> <div style="display:inline;">FLOW</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="ICC/IF"> <div style="display:inline;">ICC/IF</div> (11)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IF/IHC"> <div style="display:inline;">IF/IHC</div> (12)</div><div class="filter_line"> <input type="checkbox" value="application" 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Ratto, Simona Rapposelli, Stephan C F Neuhauss, Jingjing Zang, Silvia Rocchiccioli, Elena Michelucci, Elisa Ceccherini, Filippo M Santorelli Targeting autophagy impairment improves the phenotype of a novel CLN8 zebrafish model. Neurobiology of disease 2024-05-17 [PMID: 38763444] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/38755466" target="_blank" rel="nofollow"> Zhou Jiang, Qianpei He, Jackson Wezeman, Martin Darvas, Warren Ladiges A cocktail of rapamycin, acarbose, and phenylbutyrate prevents age-related cognitive decline in mice by targeting multiple aging pathways. GeroScience 2024-05-16 [PMID: 38755466] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/38515365" target="_blank" rel="nofollow"> Hye Kyoung Sung, Jialing Tang, James Won Suk Jahng, Erfei Song, Yee Kwan Chan, Abdul Hadee Lone, Jeffrey Peterson, Ali Abdul‐Sater, Gary Sweeney Ischemia‐induced cardiac dysfunction is exacerbated in adiponectin‐knockout mice due to impaired autophagy flux Clinical and Translational Science 2024-03-22 [PMID: 38515365] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/38504290" target="_blank" rel="nofollow"> Rebecca Z. Fan, Carolina Sportelli, Yanhao Lai, Said S. Salehe, Jennifer R. Pinnell, Harry J. Brown, Jason R. Richardson, Shouqing Luo, Kim Tieu A partial Drp1 knockout improves autophagy flux independent of mitochondrial function Molecular Neurodegeneration 2024-03-19 [PMID: 38504290] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/38005886" target="_blank" rel="nofollow"> Bai L, Zhang R, Zheng H et al. Seneca Valley Virus Degrades STING via PERK and ATF6-Mediated Reticulophagy Viruses 2023-11-03 [PMID: 38005886] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Immunohistochemistry, Western Blot" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37759678" target="_blank" rel="nofollow"> Mart�nez-Gonz�lez J, Fern�ndez-Carbonell �, Cant� A et al. Sequences of Alterations in Inflammation and Autophagy Processes in Rd1 Mice Biomolecules 2023-08-22 [PMID: 37759678] (Immunohistochemistry, Western Blot) </a></td> <td valign="top">Immunohistochemistry, Western Blot</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="IHC-Fr" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37516958" target="_blank" rel="nofollow"> Keary KM, Gu QH, Chen J, Li Z Dendritic distribution of autophagosomes underlies pathway-selective induction of LTD Cell reports 2023-07-28 [PMID: 37516958] (IHC-Fr, Mouse)<br /><br />Details:<br />Dilutions: 1:500 </a></td> <td valign="top">IHC-Fr</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="In vivo assay" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37456828" target="_blank" rel="nofollow"> Sakurai HT, Iwashita H, Arakawa S et al. Development of small fluorescent probes for the analysis of autophagy kinetics iScience 2023-07-21 [PMID: 37456828] (In vivo assay) </a></td> <td valign="top">In vivo assay</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37397563" target="_blank" rel="nofollow"> Pan Y, Yang Y, Fan M et al. Progranulin regulation of autophagy contributes to its chondroprotective effect in osteoarthritis Genes &amp; Diseases 2023-07-01 [PMID: 37397563] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37314749" target="_blank" rel="nofollow"> Amanda M, Artem P, Taylor D et al. Targeting the ATF6-mediated ER stress response and autophagy blocks integrin-driven prostate cancer progression Mol Cancer Res. 2023-06-14 [PMID: 37314749] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37131209" target="_blank" rel="nofollow"> Mitsui, K;Hishiyama, S;Jain, A;Kotoda, Y;Abe, M;Matsukawa, T;Kotoda, M; Role of macrophage autophagy in postoperative pain and inflammation in mice Journal of neuroinflammation 2023-05-02 [PMID: 37131209] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37075122" target="_blank" rel="nofollow"> S Mukhopadhy, J Encarnació, EY Lin, ASW Sohn, H Zhang, JD Mancias, AC Kimmelman Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer Science Advances, 2023-04-19;9(16):eadf9284. 2023-04-19 [PMID: 37075122] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36911373" target="_blank" rel="nofollow"> Bi Y, Yang G, Guo Z et al. Chronic high?salt intake induces cardiomyocyte autophagic vacuolization during left ventricular maladaptive remodeling in spontaneously hypertensive rats Experimental and Therapeutic Medicine 2023-02-16 [PMID: 36911373] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36872914" target="_blank" rel="nofollow"> Wan W, Qian C, Wang Q et al. STING directly recruits WIPI2 for autophagosome formation during STING-induced autophagy The EMBO journal 2023-03-06 [PMID: 36872914] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36798436" target="_blank" rel="nofollow"> Liao YH, Wu JT, Hsieh IC et al. ARMS-NF-?B signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress iScience 2023-02-17 [PMID: 36798436] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36611940" target="_blank" rel="nofollow"> Lee HJ, Kim SH, Kim YH et al. Nalfurafine Hydrochloride, a ?-Opioid Receptor Agonist, Induces Melanophagy via PKA Inhibition in B16F1 Cells Cells 2022-12-29 [PMID: 36611940] (Western Blot) </a></td> <td valign="top">Western Blot</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36284011" target="_blank" rel="nofollow"> Alan P, Vandevoorde KR, Joshi B et al. Basal Gp78-dependent mitophagy promotes mitochondrial health and limits mitochondrial ROS Cellular and Molecular Life Sciences 2022-11-01 [PMID: 36284011] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td> Karuppan MKM, Ojha CR, Rodriguez M, Lapierre J Zika virus infection during pregnancy and induced brain pathology in beclin1-deficient mouse model bioRxiv 2019-11-15 (WB, Mouse) </td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td> </td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36478958" target="_blank" rel="nofollow"> Ktena N, Kaplanis SI, Kolotuev I et al. Autophagic degradation of CNS myelin maintains axon integrity Cell stress 2022-12-01 [PMID: 36478958] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td> Ruhen O Comprehensive genomic profiling of circulating tumour DNA and tumour-derived extracellular vesicles from breast cancer patients Thesis (WB, Human) </td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36224376" target="_blank" rel="nofollow"> Li L, Zhang M, Li S et al. Isatropolone/isarubrolone Cm from Streptomyces with biological activity of inducing incomplete autophagy The Journal of antibiotics 2022-10-12 [PMID: 36224376] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36197111" target="_blank" rel="nofollow"> Zhang R, Lin H, You Q et al. Peste des Petits Ruminants Virus Upregulates STING to Activate ATF6-Mediated Autophagy Journal of virology 2022-10-26 [PMID: 36197111] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35941418" target="_blank" rel="nofollow"> Seo H, Choi HJ, Kim OH et al. The secretome obtained under hypoxic preconditioning from human adipose-derived stem cells exerts promoted anti-apoptotic potentials through upregulated autophagic process Molecular biology reports 2022-08-08 [PMID: 35941418] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Porcine"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35927699" target="_blank" rel="nofollow"> Wen Y, Chen Z, Tian Y et al. Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages Veterinary research 2022-08-04 [PMID: 35927699] (WB, Porcine) </a></td> <td valign="top">WB</td> <td valign="top">Porcine</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35786294" target="_blank" rel="nofollow"> Bai J, Liu T, Tu B et al. Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis Autophagy 2022-07-11 [PMID: 35786294] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Zebrafish"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35743315" target="_blank" rel="nofollow"> Della Vecchia S, Ogi A, Licitra R et al. Trehalose Treatment in Zebrafish Model of Lafora Disease International journal of molecular sciences 2022-06-20 [PMID: 35743315] (WB, Zebrafish) </a></td> <td valign="top">WB</td> <td valign="top">Zebrafish</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35696209" target="_blank" rel="nofollow"> Hsu MC, Guo BC, Chen CH et al. Apigenin ameliorates hepatic lipid accumulation by activating the autophagy-mitochondria pathway Journal of Food and Drug Analysis 2021-06-15 [PMID: 35696209] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35682999" target="_blank" rel="nofollow"> Ruhen O Comprehensive genomic profiling of circulating tumour DNA and tumour-derived extracellular vesicles from breast cancer patients Int J Mol Sci 2022-06-10 [PMID: 35682999] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35580604" target="_blank" rel="nofollow"> Chua JP, Bedi K, Paulsen MT et al. Myotubularin-related phosphatase 5 is a critical determinant of autophagy in neurons Current biology : CB 2022-05-10 [PMID: 35580604] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35365624" target="_blank" rel="nofollow"> Chimenti I, Picchio V, Pagano F et al. The impact of autophagy modulation on phenotype and survival of cardiac stromal cells under metabolic stress Cell death discovery 2022-04-01 [PMID: 35365624] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35316161" target="_blank" rel="nofollow"> Kao CH, Su TY, Huang WS et al. TFEB- and TFE3-dependent autophagy activation supports cancer proliferation in the absence of centrosomes Autophagy 2022-03-22 [PMID: 35316161] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34578904" target="_blank" rel="nofollow"> Sekiguchi K, Miyahara H, Inoue M et al. Metabolome Characteristics of Liver Autophagy Deficiency under Starvation Conditions in Infancy Nutrients 2021-08-29 [PMID: 34578904] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34168038" target="_blank" rel="nofollow"> Rudnick JA, Monkkonen T, Mar FA et al. Autophagy in stromal fibroblasts promotes tumor desmoplasia and mammary tumorigenesis Genes &amp; development 2021-07-01 [PMID: 34168038] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33976473" target="_blank" rel="nofollow"> MOROKI T, MATSUO S, HATAKEYAMA H et al. Databases for technical aspects of immunohistochemistry-2021 update Journal of Toxicologic Pathology 2021-02-24 [PMID: 33976473] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33915953" target="_blank" rel="nofollow"> Shi M, Shepard S, Zhou Z et al. High Dietary Phosphate Exacerbates and Acts Independently of Low Autophagy Activity in Pathological Cardiac Remodeling and Dysfunction Cells 2021-04-01 [PMID: 33915953] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot, Block/Neutralize" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33863987" target="_blank" rel="nofollow"> Inoue M, Miyahara H, Shiraishi H et al. Leucyl-tRNA synthetase deficiency systemically induces excessive autophagy in zebrafish Scientific Reports 2021-04-16 [PMID: 33863987] (Western Blot, Block/Neutralize) </a></td> <td valign="top">Western Blot, Block/Neutralize</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="In vivo assay" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33755378" target="_blank" rel="nofollow"> Wang X, Pei Z, Hossain A et al. Transcription factor-based gene therapy to treat glioblastoma through direct neuronal conversion Cancer Biology and Medicine 2021-08-15 [PMID: 33755378] (In vivo assay) </a></td> <td valign="top">In vivo assay</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33609513" target="_blank" rel="nofollow"> Xiao J, Yao J, Jia L et al. Autophagy activation and photoreceptor survival in retinal detachment Experimental eye research 2021-02-18 [PMID: 33609513] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="Western Blot, Immunocytochemistry/ Immunofluorescence" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33556283" target="_blank" rel="nofollow"> Zheng J, Hu S, Wang J et al. Icariin improves brain function decline in aging rats by enhancing neuronal autophagy through the AMPK/mTOR/ULK1 pathway Pharmaceutical Biology 2021-02-08 [PMID: 33556283] (Western Blot, Immunocytochemistry/ Immunofluorescence) </a></td> <td valign="top">Western Blot, Immunocytochemistry/ Immunofluorescence</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td> Choi G, Oh J, Lee H et al. Glucocorticoid-mediated ER-mitochondria contacts reduce AMPA receptor and mitochondria trafficking into cell terminus via microtubule destabilization. Mol. Carcinog. Nov 22 2018 (WB, Human) </td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Immunocytochemistry/ Immunofluorescence" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33526046" target="_blank" rel="nofollow"> Annangi B, Lu Z, Bruniaux J et al. Macrophage autophagy protects mice from cerium oxide nanoparticle-induced lung fibrosis Particle and Fibre Toxicology 2021-12-01 [PMID: 33526046] (Immunocytochemistry/ Immunofluorescence) </a></td> <td valign="top">Immunocytochemistry/ Immunofluorescence</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33495338" target="_blank" rel="nofollow"> Wijshake T, Zou Z, Chen B, et al. Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin Proceedings of the National Academy of Sciences of the United States of America 2021-02-02 [PMID: 33495338] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33465003" target="_blank" rel="nofollow"> Chao T, Shih HT, Hsu SC, et al. Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability Autophagy 2021-01-19 [PMID: 33465003] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33380286" target="_blank" rel="nofollow"> Zhu E, Wu H, Chen W, et al. Classical swine fever virus employs the PERK- and IRE1-dependent autophagy for viral replication in cultured cells Virulence 2021-12-01 [PMID: 33380286] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33303681" target="_blank" rel="nofollow"> Gu Q, Jiao S, Duan K et al. The BAD-BAX-caspase-3 cascade modulates synaptic vesicle pools via autophagy The Journal of neuroscience : the official journal of the Society for Neuroscience 2020-12-10 [PMID: 33303681] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33290859" target="_blank" rel="nofollow"> Pareja-Cajiao M, Gransee HM, Stowe JM et al. Age-related impairment of autophagy in cervical motor neurons Experimental gerontology 2020-12-05 [PMID: 33290859] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33277461" target="_blank" rel="nofollow"> Hwang JR, Kim WY, Cho YJ et al. Chloroquine reverses chemoresistance via upregulation of p21WAF1/CIP1 and autophagy inhibition in ovarian cancer Cell death &amp; disease 2020-12-04 [PMID: 33277461] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Drosophila"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33221768" target="_blank" rel="nofollow"> Cui L, Song W, Zeng Y et al. Deubiquitinase USP7 regulates Drosophila aging through ubiquitination and autophagy Aging (Albany NY) 2020-11-20 [PMID: 33221768] (WB, Drosophila) </a></td> <td valign="top">WB</td> <td valign="top">Drosophila</td> </tr><tr class="lined revfil hidden" data-application="ICC/IF, IP" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33146548" target="_blank" rel="nofollow"> Her JY, Lee Y, Kim SJ et al. 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Sheedy, Tathagat Dutta Ray, Katharine Cecchini, Philip D Zamore, Katey J Rayner, Yves L Marcel, Jennifer A Philips, Kathryn J Moore Mycobacterium tuberculosis induces the miR-33 locus to reprogram autophagy and host lipid metabolism Nature immunology 2016-03-16 [PMID: 27089382] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27067261" target="_blank" rel="nofollow"> Choudhury KR, Bucha S, Baksi S et al. Chaperone-like protein HYPK and its interacting partners augment autophagy. Eur. J. Cell Biol. 2016-04-01 [PMID: 27067261] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27025389" target="_blank" rel="nofollow"> Xia X, Che Y, Gao Y et al. Arginine Supplementation Recovered the IFN-gamma-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2alpha Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells. Mol Cells 2016-05-31 [PMID: 27025389] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26814135" target="_blank" rel="nofollow"> Yun Woo Jin, Kim Eun-Young, Park Ji-Eun et al. Microtubule-associated protein light chain 3 is involved in melanogenesis via regulation of MITF expression in melanocytes. Scientific Reports 2016-01-01 [PMID: 26814135] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26764007" target="_blank" rel="nofollow"> Imam S, Talley S, Nelson RS et al. TRIM5alpha degradation via autophagy is not required for retroviral restriction. J. Virol. 2016-01-13 [PMID: 26764007] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26698918" target="_blank" rel="nofollow"> Martinez-Lopez N, Garcia-Macia M, Sahu S. Autophagy in the CNS and Periphery Coordinate Lipophagy and Lipolysis in the Brown Adipose Tissue and Liver. Cell Metabolism. 2015-11-19 [PMID: 26698918] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26648164" target="_blank" rel="nofollow"> Sun Y W, Chen K Y et al. CK0403, a 9 aminoacridine, is a potent anti cancer agent in human breast cancer cells. Mol Med Rep 2016-01-01 [PMID: 26648164] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26492575" target="_blank" rel="nofollow"> Zhang F, Wang J, Chu J et al. MicroRNA-146a Induced by Hypoxia Promotes Chondrocyte Autophagy through Bcl-2. Cell. Physiol. Biochem. 2015-11-06 [PMID: 26492575] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IHC-P" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26487561" target="_blank" rel="nofollow"> Baisantry A, Bhayana S, Rong S et al. Autophagy Induces Prosenescent Changes in Proximal Tubular S3 Segments. J. Am. Soc. Nephrol. 2016-06-01 [PMID: 26487561] (IHC-P, Mouse) </a></td> <td valign="top">IHC-P</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26412641" target="_blank" rel="nofollow"> Liu L, Li CJ, Lu Y et al. Baclofen mediates neuroprotection on hippocampal CA1 pyramidal cells through the regulation of autophagy under chronic cerebral hypoperfusion. Sci Rep 2015-01-01 [PMID: 26412641] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26306675" target="_blank" rel="nofollow"> Pal I, Parida S, Prashanth Kumar BN et al. Blockade of autophagy enhances proapoptotic potential of BI-69A11, a novel Akt inhibitor, in colon carcinoma. Eur. J. Pharmacol. 2015-08-22 [PMID: 26306675] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26255724" target="_blank" rel="nofollow"> Lee YH, Chen HY, Su LJ, Chueh PJ. Sirtuin 1 (SIRT1) Deacetylase Activity and NAD(+)/NADH Ratio Are Imperative for Capsaicin-Mediated Programmed Cell Death. J. Agric. Food Chem. 2015-08-26 [PMID: 26255724] (WB, Human)<br /><br />Details:<br />ATG7 antibody was use for WB on lysates of MRC-5 normal human foetal lung fibroblast cells and adenocarcinomic human alveolar basal epithelial cell line A549 that were treated or not with Capsaicin or ethanol. </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26237736" target="_blank" rel="nofollow"> Johansson I, Monsen VT, Pettersen K et al. The marine n-3 PUFA DHA evokes cytoprotection against oxidative stress and protein misfolding by inducing autophagy and NFE2L2 in human retinal pigment epithelial cells. Autophagy. 2015-08-03 [PMID: 26237736] (WB, Human)<br /><br />Details:<br />ATG5 antibody used for WB on ARPE-19 cells that were transfected with ATG5 siRNA (Figure 4A). </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IF/IHC" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26217309" target="_blank" rel="nofollow"> Dever SM, Rodriguez M, Lapierre J et al. Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure. Front Microbiol 2015-01-01 [PMID: 26217309] (IF/IHC) </a></td> <td valign="top">IF/IHC</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Rat"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26212128" target="_blank" rel="nofollow"> Pukab K, Goldbaum O, Richter-Landsberg C et al. Mitochondrial impairment and oxidative stress compromise autophagosomal degradation of alpha-synuclein in oligodendroglial cells J. Neurochem. 2015-07-24 [PMID: 26212128] (WB, Rat)<br /><br />Details:<br />ATG5 antibody used for Western blot at a dilution of 1:500 on OLN-t40 cells (oligodendroglial cell line derived from rat brain glial cultures) that were transfected with Atg5 shRNA. </a></td> <td valign="top">WB</td> <td valign="top">Rat</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26183773" target="_blank" rel="nofollow"> Herpin A, Englberger E, Zehner M et al. Defective autophagy through epg5 mutation results in failure to reduce germ plasm and mitochondria. FASEB J 2015-10-01 [PMID: 26183773] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26120775" target="_blank" rel="nofollow"> Yang S, Long LH, Li D et al. beta-Guanidinopropionic acid extends the lifespan of Drosophila melanogaster via an AMP-activated protein kinase-dependent increase in autophagy. Aging Cell 2015-12-01 [PMID: 26120775] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26103419" target="_blank" rel="nofollow"> Till A, Saito R, Merkurjev D et al. Evolutionary trends and functional anatomy of the human expanded autophagy network. Autophagy 2015-01-01 [PMID: 26103419] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26075877" target="_blank" rel="nofollow"> Yao J, Jia L, Khan N et al. Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium Autophagy. 2015-06-03 [PMID: 26075877] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26022126" target="_blank" rel="nofollow"> Xin W, Zhao X, Liu L et al. Acetyl-CoA carboxylase 2 suppression rescues human proximal tubular cells from palmitic acid induced lipotoxicity via autophagy. Biochem Biophys Res Commun 2015-07-31 [PMID: 26022126] (Human) </a></td> <td valign="top"></td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB, ICC/IF" data-species="Rat, Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25961502" target="_blank" rel="nofollow"> Kaushik S, Cuervo AM Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis Nat Cell Biol. 2015-05-10 [PMID: 25961502] (WB, ICC/IF, Rat, Mouse) </a></td> <td valign="top">WB, ICC/IF</td> <td valign="top">Rat, Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25946189" target="_blank" rel="nofollow"> Kim Y, Kang YS, Lee NY et al. Uvrag targeting by Mir125a and Mir351 modulates autophagy associated with Ewsr1 deficiency. Autophagy. 2015-05-04 [PMID: 25946189] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25879848" target="_blank" rel="nofollow"> Wu Q, Jiang D, Huang C et al. Trehalose-mediated autophagy impairs the anti-viral function of human primary airway epithelial cells. PLoS One 2015-01-01 [PMID: 25879848] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25827954" target="_blank" rel="nofollow"> Gonnella R, Granato M, Farina A et al. PKC theta and p38 MAPK activate the EBV lytic cycle through autophagy induction. Biochim. Biophys. Acta. 2015-03-27 [PMID: 25827954] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Zebrafish"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25825498" target="_blank" rel="nofollow"> Mohanty S, Jagannathan L, Ganguli G et al. A mycobacterial phosphoribosyltransferase promotes bacillary survival by inhibiting oxidative stress and autophagy pathways in macrophages and zebrafish J. Biol. Chem. 2015-03-30 [PMID: 25825498] (WB, Zebrafish) </a></td> <td valign="top">WB</td> <td valign="top">Zebrafish</td> </tr><tr class="lined revfil hidden" data-application="WB, IHC-P" data-species="Human, Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25804753" target="_blank" rel="nofollow"> Sharifi MN, Mowers EE, Drake LE, Macleod KF. Measuring autophagy in stressed cells. Methods Mol. Biol. 2015-03-25 [PMID: 25804753] (WB, IHC-P, Human, Mouse) </a></td> <td valign="top">WB, IHC-P</td> <td valign="top">Human, Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25795167" target="_blank" rel="nofollow"> Roux C, Aligny C, Lesueur C et al. NMDA receptor blockade in the developing cortex induces autophagy-mediated death of immature cortical GABAergic interneurons: An ex vivo and in vivo study in Gad67-GFP mice. Exp. Neurol. 2015-03-17 [PMID: 25795167] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25753040" target="_blank" rel="nofollow"> Prosser SL, Morrison CG. Centrin2 regulates CP110 removal in primary cilium formation. J. Cell Biol. 2015-03-16 [PMID: 25753040] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IHC-P" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25708836" target="_blank" rel="nofollow"> Carames B, Olmer M, Kiosses WB, Lotz M. The relationship of autophagy defects and cartilage damage during joint aging in a mouse model Arthritis &amp; rheumatology. 2015-02-23 [PMID: 25708836] (IHC-P, Mouse) </a></td> <td valign="top">IHC-P</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25669150" target="_blank" rel="nofollow"> Reed M, Morris SH, Owczarczyk AB, Lukacs NW. Deficiency of autophagy protein Map1-LC3b mediates IL-17-dependent lung pathology during respiratory viral infection via ER stress-associated IL-1 Mucosal. Immunol 2015-02-11 [PMID: 25669150]<br /><br />Details:<br />Using the DyLight 550 conjugated version of NB110-53818, catalog number NB110-53818R. </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25650339" target="_blank" rel="nofollow"> Park E, Park MH, Na HS, Chung J. Xylitol induces cell death in lung cancer A549 cells by autophagy Biotechnol. Lett. 2015-02-04 [PMID: 25650339] (WB, Human)<br /><br />Details:<br />ATG5 antibody used in WB application for the detection of Atg5- Atg12 conjugates in lysates of A549 cells which were subjected to induction of autophagy with Xylitol (cells pretreated with 5 mM of 3-MA for 1 hour prior to treatments with 1 uM rapamycin or 6% xylitol for 6 hours or 24 hours). Xylitol induced autophay to a level which was comparable to rapamycin and the data is shown in Figure 5c and 5d. </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25643395" target="_blank" rel="nofollow"> Binotti B, Pavlos NJ, Riedel D et al. The GTPase Rab26 links synaptic vesicles to the autophagy pathway Elife. 2015-02-17 [PMID: 25643395] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25622137" target="_blank" rel="nofollow"> Lin HH. In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy. Chem Res Toxicol. 2015-01-29 [PMID: 25622137] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="KO, WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25587484" target="_blank" rel="nofollow"> Distinct Lysosome Phenotypes Influence Inflammatory Function in Peritoneal and Bone Marrow-Derived Macrophages. Weber K, Schilling JD Int J Inflam [PMID: 25587484] (KO, WB, Mouse) </a></td> <td valign="top">KO, WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25585051" target="_blank" rel="nofollow"> Malhotra R, Warne JP, Salas E et al. Loss of Atg12, but not Atg5, in pro-opiomelanocortin neurons exacerbates diet-induced obesity Autophagy. 2015-01-13 [PMID: 25585051] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Zebrafish"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25498006" target="_blank" rel="nofollow"> Varga M, Fodor E, Vellai T. Autophagy in zebrafish. Methods. 2014-12-09 [PMID: 25498006] (WB, Zebrafish) </a></td> <td valign="top">WB</td> <td valign="top">Zebrafish</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25485521" target="_blank" rel="nofollow"> Sun KT, Chen MY, Tu MG et al. MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation. Bone. 2014-12-05 [PMID: 25485521] (WB, Mouse)<br /><br />Details:<br />p62/SQSTM1 antibody used for WB on RAW264.7 cells subjected to RANKL and M-CSF mediated osteoclast differentiation as well as hypoxic stress (Fig. 1E ) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="ELISA" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25484078" target="_blank" rel="nofollow"> Kraya AA, Piao S, Xu X et al. Identification of secreted proteins that reflect autophagy dynamics within tumor cells. Autophagy 2015-01-01 [PMID: 25484078] (ELISA, Human) </a></td> <td valign="top">ELISA</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25375375" target="_blank" rel="nofollow"> Kao C, Chao A, Tsai Cl et al. Bortezomib enhances cancer cell death by blocking the autophagic flux through stimulating ERK phosphorylation Cell Death Dis et al. 2014-11-07 [PMID: 25375375] (WB, Human)<br /><br />Details:<br />LC3B/MAP1LC3B antibody used for WB in experiments involving human ovarian cancer cell lines TOV112D, TOV21G, OV90, SKOV3, MDAH2774, ES2, BR and BG1 cells. </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25355898" target="_blank" rel="nofollow"> El-Hage N, Rodriguez M, Dever Sm et al. HIV-1 and morphine regulation of autophagy in microglia: Limited interactions in the context of HIV-1 infection and opioid abuse J. Virol. 2014-10-29 [PMID: 25355898] (WB, Human)<br /><br />Details:<br />ATG5 antibody used for WB at 1:1000 dilution on lysates of primary human microglial cells that were treated or not with HIV-1SF162 macrophage (M)-tropic strain and/or Morphine for 1, 3 or 5 days (Figure 1B). </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB, IHC-P" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25341032" target="_blank" rel="nofollow"> Lin Hh, Lin Sm, Chung Y et al. Dynamic involvement of ATG5 in cellular stress responses. Cell Death Dis. 2014-10-24 [PMID: 25341032] (WB, IHC-P, Mouse) </a></td> <td valign="top">WB, IHC-P</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25323583" target="_blank" rel="nofollow"> Rozman S, Yousefi S, Oberson K et al. The generation of neutrophils in the bone marrow is controlled by autophagy. Cell Death Differ. 2014-10-17 [PMID: 25323583] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25293350" target="_blank" rel="nofollow"> Liao Cl, Chen Cm, Chang Yz et al. Pine (Pinus morrisonicola Hayata) needle Extracts Sensitize GBM8901 Human Glioblastoma Cells to Temozolomide by Downregulating Autophagy and O(6)-Methylguanine-DnA Methyltransferase Expression. J. Agric. Food Chem. 2014-10-29 [PMID: 25293350] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Primate"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25142602" target="_blank" rel="nofollow"> Granato M, Santarelli R, Farina A et al. EBV blocks the autophagic flux and appropriates the autophagic machinery to enhance viral replication. J Virol. 2014-08-20 [PMID: 25142602] (WB, Primate) </a></td> <td valign="top">WB</td> <td valign="top">Primate</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25046114" target="_blank" rel="nofollow"> Ligeon LA, Moreau K, Barois N et al. Role of VAMP3 and VAMP7 in the commitment of Yersinia pseudotuberculosis to LC3-associated pathways involving single- or double-membrane vacuoles. Autophagy 2014-09-01 [PMID: 25046114] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24928681" target="_blank" rel="nofollow"> Montaigne D, Marechal X, Coisne A et al. Myocardial Contractile Dysfunction is Associated with Impaired Mitochondrial Function and Dynamics in Type 2 Diabetic but not in Obese Patients. Circulation. 2014-06-13 [PMID: 24928681] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Bovine"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24895572" target="_blank" rel="nofollow"> Zielniok K, Motyl T, Gajewska M. Functional Interactions between 17beta-Estradiol and Progesterone Regulate Autophagy during Acini Formation by Bovine Mammary Epithelial Cells in 3D Cultures. BioMed Research International. 2014-05-07 [PMID: 24895572] (WB, Bovine) </a></td> <td valign="top">WB</td> <td valign="top">Bovine</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24875860" target="_blank" rel="nofollow"> Yang Annan, Rajeshkumar N V, Wang Xiaoxu et al. 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Requirement of autophagy proteins Beclin-1 and Map1-LC3b in the induction of innate and adaptive immune responses to respiratory syncytial virus infection Thesis 2014-01-01 (ICC/IF, Mouse)<br /><br />Details:<br />This reference used the DyLight 550 version of NB110-53818. </td> <td valign="top">ICC/IF</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24100123" target="_blank" rel="nofollow"> Roy K, Raychaudhuri M, Chakrabarti O, Mukhopadhyay D. Growth Factor Receptor-Bound Protein 2 Promotes Autophagic Removal of Amyloid-beta Protein Precursor Intracellular Domain Overload in Neuronal Cells. 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J Dent Res 2013-07-24 [PMID: 23884556] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23800883" target="_blank" rel="nofollow"> Zhang C, He Y, Okutsu M et al. Autophagy is involved in adipogenic differentiation by repressesing proteasome-dependent PPARy2 degradation. Am J Physiol Endocrinol Metab. 2013-08-01 [PMID: 23800883] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23768496" target="_blank" rel="nofollow"> Benjamin JL, Sumpter R Jr, Levine B, Hooper LV. Intestinal Epithelial Autophagy Is Essential for Host Defense against Invasive Bacteria. Cell Host Microbe 2013-06-12 [PMID: 23768496] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23740229" target="_blank" rel="nofollow"> Ciccia F, Accardo-Palumbo A, Rizzo A et al. Evidence that autophagy, but not the unfolded protein response, regulates the expression of IL-23 in the gut of patients with ankylosing spondylitis and subclinical gut inflammation. Ann Rheum Dis 2013-06-05 [PMID: 23740229] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23733886" target="_blank" rel="nofollow"> Sorrentino V, Nelson JK, Maspero E et al. The LXR-IDOL axis defines a clathrin, caveolae, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation. 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Nature. 2013-02-14 [PMID: 23364696]<br /><br />Details:<br />Tat-beclin 1 (L-amino acid) / Tat-Beclin 1 L11 (NBP2-49886) and Tat–beclin 1 (D-amino acid)/Tat-Beclin 1 D11, Retroinverso form (NBP2-49888) along with the control peptide Tat-scrambled (L-amino acid)/Tat-Beclin 1 L11S Peptide, Scrambled Control (NBP2-49887) were tested in-vivo for the induction of autophagy. 6-week-old GFP-LC3 transgenic mice, and normal or CHIKV and WNV Egypt strain virus infected 5-day-old GFP-LC3 mice were injected intra-peritoneally (i.p.) with these Tat-beclin derivative peptides at a dose of 20 mg/Kg body weight (5.3uM/Kg). Brain tissues were analyzed using IHC-Frozen and Western blot analysis for measuring cell death (TUNEL Assay) and p62 expression respectively. Toxicity of these peptides was assessed in 6-day-old C57BL/6J mice via daily injections of Tat-scrambled (L-amino acid, Tat-Beclin 1 L11S) or Tat-beclin 1 (L-amino acid, Tat-Beclin 1 L11) at 15 mg kg-1 and Tat–beclin 1 (D-amino acid, Tat-Beclin 1 D11) at a dose of 20 mg/Kg body weight for 2 weeks (Figure 4). </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23359526" target="_blank" rel="nofollow"> Brennan GP, Jimenez-Mateos EM, McKiernan RC et al. Transgenic Overexpression of 14-3-3 Zeta Protects Hippocampus against Endoplasmic Reticulum Stress and Status Epilepticus In Vivo. 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J Biol Chem 2013-01-22 [PMID: 23341467] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23242206" target="_blank" rel="nofollow"> Zhu S, Cao L, Yu Y et al. Inhibiting autophagy potentiates the anticancer activity of IFN1@/ IFNalpha in chronic myeloid leukemia cells. Autophagy 2012-12-14 [PMID: 23242206] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23195496" target="_blank" rel="nofollow"> Kang R, Tang D, Lotze MT, Zeh Iii HJ. Autophagy is required for IL-2-mediated fibroblast growth Exp Cell Res 2012-11-27 [PMID: 23195496] (Mouse) </a></td> <td valign="top"></td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IF/IHC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23039021" target="_blank" rel="nofollow"> Curtis S, Jones CJ, Garrod A et al. Identification of Autophagic Vacuoles and Regulators of Autophagy in Villous Trophoblast from Normal Term Pregnancies and in Fetal Growth Restriction J Matern Fetal Neonatal Med 2012-10-08 [PMID: 23039021] (IF/IHC, Human) </a></td> <td valign="top">IF/IHC</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22934255" target="_blank" rel="nofollow"> Fujii S, Hara H, Araya J et al. Insufficient autophagy promotes bronchial epithelial cell senescence in chronic obstructive pulmonary disease. Oncoimmunology. [PMID: 22934255] (WB, Human)<br /><br />Details:<br />Citation using the HRP form of this antibody. </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22929803" target="_blank" rel="nofollow"> Zang Y, Thomas SM, Chan ET et al. Carfilzomib and ONX 0912 Inhibit Cell Survival and Tumor Growth of Head and Neck Cancer and Their Activities Are Enhanced by Suppression of Mcl-1 or Autophagy Clin Cancer Res 2012-10-15 [PMID: 22929803] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22875977" target="_blank" rel="nofollow"> Chakrabarti A, Ghosh PK, Banerjee S et al. RNase L Triggers Autophagy in Response to Viral Infections J Virol 2012-10-01 [PMID: 22875977] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22840404" target="_blank" rel="nofollow"> Cannizzo ES, Clement CC, Morozova K et al. Age-Related Oxidative Stress Compromises Endosomal Proteostasis Cell Rep 2012-07-26 [PMID: 22840404] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22732841" target="_blank" rel="nofollow"> Sandilands E, Serrels B, Wilkinson S, Frame MC. Src-dependent autophagic degradation of Ret in FAK-signalling-defective cancer cells EMBO Rep 2012-06-26 [PMID: 22732841] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22730392" target="_blank" rel="nofollow"> Garcia AG, Wilson RM, Heo J et al. Interferon-gamma ablation exacerbates myocardial hypertrophy in diastolic heart failure Am J Physiol Heart Circ Physiol 2012-09-01 [PMID: 22730392] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22509024" target="_blank" rel="nofollow"> Kang R, Loux T, Tang D, Schapiro NE, Vernon P, Livesey KM, Krasinskas A, Lotze MT, Zeh HJ 3rd. The expression of the receptor for advanced glycation endproducts (RAGE) is permissive for early pancreatic neoplasia. Proc Natl Acad Sci U S A;109(18):7031-6. 2012-05-01 [PMID: 22509024] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22440612" target="_blank" rel="nofollow"> Razani B, Feng C, Coleman T et al. Autophagy Links Inflammasomes to Atherosclerotic Progression Cell Metabolism 2012-04-04 [PMID: 22440612] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22405854" target="_blank" rel="nofollow"> Sharara-Chami RI, Zhou Y, Ebert S et al. Epinephrine deficiency results in intact glucose counter-regulation, severe hepatic steatosis and possible defective autophagy in fasting mice The International Journal of Biochemistry &amp; Cell Biology 2012-06-01 [PMID: 22405854] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22395361" target="_blank" rel="nofollow"> Yu Y, Yang L, Zhao M et al. Targeting microRNA-30a-mediated autophagy enhances imatinib activity against human chronic myeloid leukemia cells Leukemia 2012-03-07 [PMID: 22395361] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22223827" target="_blank" rel="nofollow"> Zhang Y, Morgan MJ, Chen K et al. Induction of autophagy is essential for monocyte-macrophage differentiation. Blood. 2012-01-05 [PMID: 22223827] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22150682" target="_blank" rel="nofollow"> Vigano A, Vasso M, Caretti A, Bravata V, Terraneo L, Fania C, Capitanio D, Samaja M, Gelfi C. Protein modulation in mouse heart under acute and chronic hypoxia. Proteomics;11(21):4202-17. 2011-11-01 [PMID: 22150682] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22127234" target="_blank" rel="nofollow"> Cufi S, Vazquez-Martin A, Oliveras-Ferraros C et al. Autophagy positively regulates the CD44(+) CD24(-/low) breast cancer stem-like phenotype. Cell Cycle 2011-11-15 [PMID: 22127234] (Human) </a></td> <td valign="top"></td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21954231" target="_blank" rel="nofollow"> Lee H, Noh J-Y, Oh Y et al. IRE1 plays an essential role in ER stress-mediated aggregation of mutant huntingtin via the inhibition of autophagy flux. Human Molecular Genetics. 2011-09-27 [PMID: 21954231] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21946213" target="_blank" rel="nofollow"> Liang JJ, Yu CY, Liao CL, Lin YL. Autophagy is involved in the early step of Japanese encephalitis virus infection. Microbes Infect 2011-01-01 [PMID: 21946213] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21690574" target="_blank" rel="nofollow"> Ling Y-H, Aracil M, Zou Y et al. PM02734 Induces Caspase-Independent Cell Death Associated with Features of Autophagy, Inhibition of the Akt/mTOR Signaling Pathway, and Activation of DAP Kinase. Clin Cancer Res. 2011-06-20 [PMID: 21690574] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21572516" target="_blank" rel="nofollow"> Raveh B, London N, Zimmerman L, Schueler-Furman O. Rosetta FlexPepDock ab-initio: simultaneous folding, docking and refinement of peptides onto their receptors. PLoS One 2011-01-01 [PMID: 21572516] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21555592" target="_blank" rel="nofollow"> Dash R, Azab B, Quinn B et al. Apogossypol derivative BI-97C1 (Sabutoclax) targeting Mcl-1 sensitizes prostate cancer cells to mda-7/IL-24-mediated toxicity Proc Natl Acad Sci U S A 2011-05-24 [PMID: 21555592] (WB, Human) </a></td> <td valign="top">WB</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ICC/IF" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21363932" target="_blank" rel="nofollow"> Lee J-E, Oh H-A, Song H et al. Autophagy Regulates Embryonic Survival During Delayed Implantation. Endocrinology;152(5):2067-75. 2011-05-01 [PMID: 21363932] (ICC/IF, Mouse) </a></td> <td valign="top">ICC/IF</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21353614" target="_blank" rel="nofollow"> Elgendy M, Sheridan C, Brumatti G et al. Oncogenic Ras-induced expression of Noxa and Beclin-1 promotes autophagic cell death and limits clonogenic survival. Mol Cell. 2011-04-01 [PMID: 21353614] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse, Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21342660" target="_blank" rel="nofollow"> Herd HL, Malugin A, Ghandehari H. Silica nanoconstruct cellular toleration threshold in vitro. J Control Release;153(1):40-8. 2011-07-15 [PMID: 21342660] (WB, Mouse, Human) </a></td> <td valign="top">WB</td> <td valign="top">Mouse, Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21228817" target="_blank" rel="nofollow"> Wang RC, Levine B. Calcipotriol Induces Autophagy in HeLa Cells and Keratinocytes. J Invest Dermatol. 2011-01-01 [PMID: 21228817] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21075354" target="_blank" rel="nofollow"> Mostowy S, Bonazzi M, Hamon MA et al. Entrapment of intracytosolic bacteria by septin cage-like structures. Cell Host Microbe 2010-11-18 [PMID: 21075354] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="ICC/IF, WB" data-species="Mouse, Primate"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21062285" target="_blank" rel="nofollow"> Brady OA, Meng P, Zheng Y et al. egulation of TDP-43 aggregation by phosphorylation and p62/SQSTM1 J Neurochem 2011-01-01 [PMID: 21062285] (ICC/IF, WB, Mouse, Primate) </a></td> <td valign="top">ICC/IF, WB</td> <td valign="top">Mouse, Primate</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/19898471" target="_blank" rel="nofollow"> Travassos LH, Carneiro LA, Ramjeet M et al. Nod1 and Nod2 direct autophagy by recruiting ATG16L1 to the plasma membrane at the site of bacterial entry. Nat Immunol 2010-01-01 [PMID: 19898471] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/19654319" target="_blank" rel="nofollow"> Fujimoto K, Hanson PT, Tran H et al. Autophagy Regulates Pancreatic Beta Cell Death in Response to Pdx1 Deficiency and Nutrient Deprivation. J Biol Chem;284(40):27664-27673. 2009-01-01 [PMID: 19654319] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/19571141" target="_blank" rel="nofollow"> Lee J-A, Gao F-B. Inhibition of Autophagy Induction Delays Neuronal Cell Loss Caused by Dysfunctional ESCRT-III in Frontotemporal Dementia. J Neurosci. 2009-01-01 [PMID: 19571141] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/19305394" target="_blank" rel="nofollow"> English L, Chemali M, Duron J et al. Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection. Nat Immunol 2009-05-01 [PMID: 19305394] (WB, Mouse) </a></td> <td valign="top">WB</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/18849966" target="_blank" rel="nofollow"> Cadwell K, Liu JY, Brown SL et al. A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells. Nature;456(7219):259-63. 2008-11-13 [PMID: 18849966] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species="Mouse, Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/18196969" target="_blank" rel="nofollow"> Pyo JO, Nah J, Kim HJ et al. Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death. Autophagy;4(3):315-21. 2008-04-01 [PMID: 18196969] (WB, Mouse, Human) </a></td> <td valign="top">WB</td> <td valign="top">Mouse, Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/18073215" target="_blank" rel="nofollow"> Wang, Y, R Singh et al. Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus. J. Biol. Chem. 283: 4766-4777. 2008-01-01 [PMID: 18073215] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr><td colspan="3" align="center"> <a class="pubs_showall">Show All 285 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a> </td></tr></tbody> </table> </div> </div><a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>ATG5 Antibody (NB110-53818)</span> (8) <span style="display:none;">4.3</span><span style="display:none;">8</span></h2> <div class="ds_info"> <div class="bottom15"> <strong>Average Rating: 4.3</strong> <div class="dsSprite_stars4"></div> (Based on 8 reviews) </div> <div><div class="ds_info">We have 8 reviews tested in 3 species: Human, Mouse, Rat.</div> <div class="align-right"> <button class="greybutton btn_width" id="novusreview" name="novusreview" onclick="window.location='/reviews/NB110-53818'"; >Submit a Review</button><br /> </div> </div> <div class="blueHead"><strong>Reviews using NB110-53818:</strong></div> <div class="filterParent"><div class="reviews_filterList"> <div class="blueHead">Filter by Applications</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="WB"> <div style="display:inline;">WB</div> (7)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="IHC-P"> <div style="display:inline;">IHC-P</div> (1)</div><div class="filter_clear" data-type="review" data-filter="application">All Applications</div></div> <div class="reviews_filterList"> <div class="blueHead">Filter by Species</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (2)</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (4)</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Rat"> <div style="display:inline;">Rat</div> (1)</div><div class="filter_clear" data-type="review" data-filter="species">All Species</div></div></div><div id="reviews_list"> <table class="wide lightPad tablesorter" id="reviews_list_table"> <thead> <tr> <th>Images</th> <th>Ratings</th> <th>Applications</th> <th>Species</th> <th>Date</th> <th>Details</th> </tr> </thead> <tbody><tr class="top-lined revfil" data-rating="Excellent" data-application="WB" data-species="Human"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nb110-53818_61781.jpg" title="W-B of the lysate of DU145 cells - control and ATG5 KD" class="colorbox" title="Western Blot" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nb110-53818_61781.jpg" class="reviews_imageBorder" width="75" alt="Western Blot ATG5 NB110-53818" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="06/27/2023" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />Armen Petrosyan</div> </td> <td title="Western Blot">WB</td> <td>Human</td> <td>06/27/2023</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>DU145 human prostate carcinoma cell line</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr><tr><td class="text_bold">Lot</td><td>P</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Mouse"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nb110-53818_53501_0_0.png" title="WT Liver samples" class="colorbox" title="Western Blot" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nb110-53818_53501_0_0.png" class="reviews_imageBorder" width="75" alt="Western Blot ATG5 NB110-53818" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlassBlue"></div> Enlarge</span></a><meta content="09/15/2020" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>09/15/2020</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>Liver tissue</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr><tr><td class="text_bold">Lot</td><td>I-1</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Mouse"> <td>&nbsp;<meta content="03/31/2018" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>03/31/2018</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>Mouse Colon</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr><tr><td class="text_bold">Lot</td><td>M-1</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Human"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nb110-53818_38091_0.jpg" title="100 ng/ml LPS treated human primary hepatocytes" class="colorbox" title="Western Blot" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nb110-53818_38091_0.jpg" class="reviews_imageBorder" width="75" alt="Western Blot ATG5 NB110-53818" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlassBlue"></div> Enlarge</span></a><meta content="12/15/2017" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Human</td> <td>12/15/2017</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>human parimary hepatocytes,Human primary hepatocytes</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr><tr><td class="text_bold">Lot</td><td>K-3</td></tr></table><h2 class="greyHead">Comments</h2> <table class="even wide top"><tr><td class="text_bold">Comments</td><td>100 ng/ml LPS treated human primary hepatocytes</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="IHC-P" data-species=""> <td><a href="https://images.novusbio.com/reviews/Immunohistochemistry-Paraffin_ATG5-Antibody-(NB110-53818)-(01-ml)_NB110-53818_10841.jpg" title="Mouse knee" class="colorbox" title="Immunohistochemistry-Paraffin" > <img loading="lazy" src="https://images.novusbio.com/reviews/Immunohistochemistry-Paraffin_ATG5-Antibody-(NB110-53818)-(01-ml)_NB110-53818_10841.jpg" class="reviews_imageBorder" width="75" alt="Immunohistochemistry-Paraffin ATG5 NB110-53818" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="10/04/2014" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Merissa Olmer</div> </td> <td title="Immunohistochemistry-Paraffin">IHC-P</td> <td></td> <td>10/04/2014</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunohistochemistry-Paraffin</td></tr><tr><td class="text_bold">Lot</td><td>E-3</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Mouse"> <td><a href="https://images.novusbio.com/reviews/review_image_24061_3696_1348236262.jpg" title="Western Blot image for NB110-53818" class="colorbox" title="Western Blot" > <img loading="lazy" src="https://images.novusbio.com/reviews/review_image_24061_3696_1348236262.jpg" class="reviews_imageBorder" width="75" alt="Western Blot ATG5 NB110-53818" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlassBlue"></div> Enlarge</span></a><meta content="09/21/2012" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Ann Marie Santiago</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>09/21/2012</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>Murine hepatoma</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr><tr><td class="text_bold">Lot</td><td>e-2</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Rat"> <td><a href="https://images.novusbio.com/reviews/review_image_16486_1786_1324414494.jpg" title="Western Blot image for NB110-53818" class="colorbox" title="Western Blot" > <img loading="lazy" src="https://images.novusbio.com/reviews/review_image_16486_1786_1324414494.jpg" class="reviews_imageBorder" width="75" alt="Western Blot ATG5 NB110-53818" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="12/20/2011" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Rat</td> <td>12/20/2011</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>Rat Cardiomyocyte</td></tr><tr><td class="text_bold">Species</td><td>Rat</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Excellent" data-application="WB" data-species="Mouse"> <td>&nbsp;<meta content="11/16/2011" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>11/16/2011</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>Mouse</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr></tbody> </table> </div><br /> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info"> View specific protocols for ATG5 Antibody (NB110-53818): <ul><li><a href="/support/protocols/western-blot-protocol-for-atg5-antibody-nb110-53818">Western Blot protocol for ATG5 Antibody (NB110-53818)</a></li></ul>Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/elisa/protocol.html">ELISA</a></li><li><a href="/support/support-by-application/flow-cytometry/protocol.html">Flow Cytometry</a></li><li><a href="/support/support-by-application/immunocytochemistry/protocol.html">Immunocytochemistry/ Immunofluorescence</a></li><li><a href="/support/support-by-application/immunohistochemistry-paraffin/protocol.html">Immunohistochemistry-Paraffin</a></li><li><a href="/support/support-by-application/immunoprecipitation/protocol.html">Immunoprecipitation</a></li><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&amp;wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div><div class="movie-cell"> <span class="movie-title">ICC/IF Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=5AcrhhHmQZs" /> <a href="https://www.youtube.com/embed/5AcrhhHmQZs?rel=0&amp;wmode=transparent" class="youtube"> <div class="reduce sprite_ICC-IFvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for ATG5 Antibody (NB110-53818). (Showing 1 - <span id="faqcnt">5</span> of 5 FAQs).</h2> <a id="faqs"></a> <ol id="faq_list"><li class="faqwrap "> <span class="faq_question">I am interested in testing your anti ATG5 (NB110-53818SS) for Immunofluorescence in MDCK cell (canine epithelial cell line). I noticed you report reactivity of the antibody against several species but not dog. Do you know if the Ab will react against canine genome? Also for IF what fixation do you recommend?</span> <ul class="faq_answer"> <li>The applications and species listed for any one of our antibodies are ones we have tested and validated. Canine is not listed as it has not been tested and therefore we cannot guarantee its cross reactivity. If you would be interested in testing this antibody in canine, we can recommend our Innovator's Reward. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. Learn more about our Innovators Reward Program. Also, please use this link to view our Immunofluorescence protocol which explains the fixation method that we recommend.</li> </ul> </li><li class="faqwrap "> <span class="faq_question">Our customer is interested in this ATG5 antibody (cat. no. NB110-53818). Would you please confirm that this antibody would recognize ATG5 alone, as well as ATG5-ATG12 conjugate?</span> <ul class="faq_answer"> <li>The antibody was raised against a peptide corresponding to the N-terminal of ATG5. It should recognize ATG5 alone but it appears that ATG5 is rarely found alone in the cell.</li> </ul> </li><li class="faqwrap "> <span class="faq_question">The arrow in the data sheet points to the ATG5/ATG12 band of 56 kDa. There is also a band of 33 kDa which I guess is ATG5? Could you explain the meaning of detecting the ATG5/ATG12 conjugate in preference to ATG5?  Does the antibody cross-react with ATG12?</span> <ul class="faq_answer"> <li>The Western blot image you are referring to illustrates the ATG5/ATG12 conjugate, which is formed during autophagy. During autophagy ATG5 is covalently bonds ATG12 in a ubiquitous reaction. The lysate that was used was meant to illustrate the ability to detect autophagy by Western blot via ATG5/ATG12. It is possible to detect free ATG5 in cell lysates, although it is not seen in this Western blot. The lower 2 bands do not correspond to ATG5. Our ATG5 antibody does not detect ATG12, which runs at about 23 kDa.</li> </ul> </li><li class="faqwrap "> <span class="faq_question">Does this antibody detect free ATG5 monomer in HeLa or HEK 293 cells? </span> <ul class="faq_answer"> <li>This antibody is able to detect ATG5 (unconjugated form) at ~33 kDa and the ATG12-conjugated form at ~56 kDa. In the Western blot image we have on our website, we show the antibody's ability to detect the autophagy induced conjugate ATG5/ATG12.  A customer review image shows both the conjugated and unconjugated ATG5 in rat cardiomyocytes. Several  publications show this antibody working in Western blot with mouse and human samples. We are not sure if in any of the publications HeLa or HEK 293 cells specifically were used, but if the ATG5 protein is present, this product will detect it in Western blot.</li> </ul> </li><li class="faqwrap "> <span class="faq_question">Would you please help confirm if NB110-53818 has been tested by IHC with frozen sections?</span> <ul class="faq_answer"> <li>NB110-53818 has been tested with paraffin-embedded tissue, but we have not validated this antibody for IHC with frozen tissue sections.</li> </ul> </li></ol> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a> <a id="positivecontrollysates"></a> <h2 class="greyHead2">Control Lysate(s)</h2> <div class="ds_info"> <table class="wide"><tr class="lined"> <td><a href="atg5-lysate_nbl1-07799">ATG5 Overexpression Lysate</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NBL1-07799" data-return="atg5-antibody_nb110-53818#supportresearch" data-produrl="atg5-antibody_nb110-53818" data-catnums="NBL1-07799" ></div> </td> </tr> </table> </div><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Rabbit&amp;facet_conjugate=HRP">Anti-Rabbit HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&amp;facet_conjugate=FITC">Anti-Rabbit FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&amp;facet_conjugate=Biotin">Anti-Rabbit Biotin Labeled Antibodies</a></li><li>&nbsp;</li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Rabbit%22">View all of our anti-Rabbit Secondary Antibodies</a></li> </ul> </div></td><td width="5">&nbsp;</td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Rabbit%22">Rabbit Isotype Controls</a></li><li>&nbsp;</li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional ATG5 Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array NB110-53818</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/atg5">ATG5 Antibodies</a></li><li> <a href="/lysates/atg5">ATG5 Lysate</a></li><li> <a href="/proteins/atg5">ATG5 Proteins</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr><td valign="top"><h2 class="greyHead2">Research Areas for ATG5 Antibody (NB110-53818)</h2> <a id="research_areas"></a> <div class="ds_info">Find related products by research area.</div> <div class="ds_info"> <ul class="ds_noDotList"><li class="left width"><a href="/research-areas/apoptosis">Apoptosis</a></li><li class="left width"><a href="/research-areas/autophagy">Autophagy</a></li><li class="left width"><a href="/research-areas/cancer">Cancer</a></li><li class="left width"><a href="/research-areas/Cardiovascular-Biology">Cardiovascular Biology</a></li><li class="left width"><a href="/research-areas/cell-biology">Cell Biology</a></li><li class="left width"><a href="/research-areas/protein-turnover-ubiquitin-proteasome-pathway">Ubiquitin Proteasome Pathway</a></li></ul> </div></td></tr></table><h2 class="greyHead2">Blogs on ATG5. <span class="blogdiv">Showing 1-<span id="blogcnt">10</span> of 32 blog posts - <a class="blogs_showall">Show all blog posts</a>.</span></h2> <a id="blogsect"></a> <div class="ds_info"><a href="/antibody-news/tag/atg5">Check out the latest blog posts on ATG5</a>.</div> <div class="ds_info"> <table id="blogs_list_table"> <tbody> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/liver-ask1-activates-autophagy-to-protect-against-hepatic-fat-accumulation-non-alcoholic-steatohepatitis-and-fibrosis.">Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis</a></strong><br/> <span> By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...&nbsp;&nbsp;<a href="/antibody-news/liver-ask1-activates-autophagy-to-protect-against-hepatic-fat-accumulation-non-alcoholic-steatohepatitis-and-fibrosis.">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href=""></a></strong><br/> <span>&nbsp;&nbsp;<a href="">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href=""></a></strong><br/> <span>&nbsp;&nbsp;<a href="">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href=""></a></strong><br/> <span>&nbsp;&nbsp;<a href="">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/animal-models-to-study-autophagy">Animal Models to Study Autophagy</a></strong><br/> <span> By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...&nbsp;&nbsp;<a href="/antibody-news/animal-models-to-study-autophagy">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/losing-memory-toxicity-from-mutant-app-and-amyloid-beta-explain-the-hippocampal-neuronal-damage-in-alzheimers-disease">Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease</a></strong><br/> <span> &nbsp;By Jamshed Arslan Pharm.D. &nbsp;Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...&nbsp;&nbsp;<a href="/antibody-news/losing-memory-toxicity-from-mutant-app-and-amyloid-beta-explain-the-hippocampal-neuronal-damage-in-alzheimers-disease">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href=""></a></strong><br/> <span>&nbsp;&nbsp;<a href="">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/autophagy-independent-roles-of-the-core-atg-proteins">Autophagy independent roles of the core ATG proteins</a></strong><br/> <span> By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...&nbsp;&nbsp;<a href="/antibody-news/autophagy-independent-roles-of-the-core-atg-proteins">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/antibodies/key-targets-in-apoptosis-necroptosis-and-autophagy">Key Targets in Apoptosis, Necroptosis, and Autophagy</a></strong><br/> <span>Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and ...&nbsp;&nbsp;<a href="/antibody-news/antibodies/key-targets-in-apoptosis-necroptosis-and-autophagy">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/antibodies/the-role-of-parkin-and-autophagy-in-retinal-pigment-epithelial-cell-rpe-degradation">The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation</a></strong><br/> <span>The root of Parkinson&rsquo;s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1, ...&nbsp;&nbsp;<a href="/antibody-news/antibodies/the-role-of-parkin-and-autophagy-in-retinal-pigment-epithelial-cell-rpe-degradation">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/the-use-of-actin-as-a-loading-control-in-research-on-fruiting-body-development-and-vegetative-growth-in-sordaria-macrospora-research">The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research</a></strong><br/> <span>Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...&nbsp;&nbsp;<a href="/antibody-news/antibodies/the-use-of-actin-as-a-loading-control-in-research-on-fruiting-body-development-and-vegetative-growth-in-sordaria-macrospora-research">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/required-proteins-for-p62-sqstm1-regulation-and-a-role-for-p62-sqstm1-in-neuronal-autophagy">Required proteins for p62/SQSTM1 regulation and a role for p62/SQSTM1 in neuronal autophagy</a></strong><br/> <span>Autophagy is a crucial cellular process that clears the cell of protein aggregates, toxins, and damaged cell products. Accumulation of toxins, damaged cell products and unwanted proteins has been proven to play a role in aging and many forms of dis...&nbsp;&nbsp;<a href="/antibody-news/antibodies/required-proteins-for-p62-sqstm1-regulation-and-a-role-for-p62-sqstm1-in-neuronal-autophagy">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/the-role-of-lc3b-and-autophagy-in-alcohol-induced-liver-disease">The role of LC3B and autophagy in alcohol induced liver disease </a></strong><br/> <span>Autophagy is a crucial intracellular pathway that manages the degradation and recycling of long-lived proteins in the cell. The LC3 (or light chain 3) family is composed of three members, LC3A, LC3B and LC3C. Upon autophagy induction, LC3 is cleave...&nbsp;&nbsp;<a href="/antibody-news/antibodies/the-role-of-lc3b-and-autophagy-in-alcohol-induced-liver-disease">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/wipi1-an-essential-regulator-of-early-autophagosome-assembly">WIPI1 - An essential regulator of early autophagosome assembly</a></strong><br/> <span>WD repeat domain phosphoinositide-interacting protein 1 (WIPI) is involved in the lysosomal degradation of cytoplasmic components during starvation-induced autophagy. WIPI1 is a seven bladed beta-propeller protein that provides a scaffold for the ...&nbsp;&nbsp;<a href="/antibody-news/antibodies/wipi1-an-essential-regulator-of-early-autophagosome-assembly">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg4c-a-regulator-of-the-early-steps-of-autophagosome-assembly">ATG4C - A regulator of the early steps of autophagosome assembly</a></strong><br/> <span>Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg4c-a-regulator-of-the-early-steps-of-autophagosome-assembly">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg16l2-an-autophagy-related-protein-with-unknown-functions">ATG16L2 - An autophagy-related protein with unknown functions</a></strong><br/> <span>Autophagy is a process by which cells degrade and recycle damaged organelles or misfolded proteins. These various cargo are engulfed in a double-membrane structure called the autophagosome. The autophagosome then fuses with the lysosome to facilit...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg16l2-an-autophagy-related-protein-with-unknown-functions">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg4d-a-regulator-of-autophagy-and-apoptosis">ATG4D - A regulator of autophagy and apoptosis</a></strong><br/> <span>Autophagy is an essential cellular process whereby damaged proteins and organelles are degraded and recycled. Autophagy, while happening constantly at a basal level, is tightly regulated and can be further induced under cellular stress. One of the ...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg4d-a-regulator-of-autophagy-and-apoptosis">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg4b-a-cysteine-protease-involved-in-autophagosome-elongation">ATG4B - a cysteine protease involved in autophagosome elongation</a></strong><br/> <span>Autophagy can be broken down into 4 main stages: phagophore nucleation, autophagosome elongation, autophagosome docking and fusion with a lysosome, and vesicle breakdown and degradation. ATG4B is one of four ATG4 homologs (ATG4A, ATG4B, ATG4C, and ...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg4b-a-cysteine-protease-involved-in-autophagosome-elongation">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg16l1-a-key-player-in-the-development-of-the-autophagosome">ATG16L1 - a key player in the development of the autophagosome</a></strong><br/> <span>Like apoptosis, autophagy is a highly regulated physiologic process that involves cellular degradation and recycling of organelles and macromolecules. Autophagy is a survival mechanism induced by states of stress, starvation, and infection. A do...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg16l1-a-key-player-in-the-development-of-the-autophagosome">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg5-an-essential-regulator-of-autophagosome-assembly">ATG5 - an essential regulator of autophagosome assembly</a></strong><br/> <span>Autophagy is important for the removal of damaged organelles or proteins as well as for the regulation of cellular homeostasis in response to stress. Proteins or organelles that are targeted for degradation are engulfed in a double-membrane structu...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg5-an-essential-regulator-of-autophagosome-assembly">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg7-an-e1-enzyme-for-the-ubiquitin-like-autophagy-proteins">ATG7 - an E1 enzyme for the ubiquitin-like autophagy proteins</a></strong><br/> <span>Autophagy is an essential cellular process that maintains homeostasis through the degradation and recycling of cytoplasmic organelles and macromolecules. Substrates targeted for autophagy are engulfed in a double-membrane structure called the auto...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg7-an-e1-enzyme-for-the-ubiquitin-like-autophagy-proteins">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/p62-sqstm1-targeting-ubiquitinated-proteins-for-autophagic-degradation">p62/SQSTM1 - targeting ubiquitinated proteins for autophagic degradation </a></strong><br/> <span>During autophagy ubiquitinated cargo or substrates are engulfed in a double-membrane autophagosome and transported to the lysosome for degradation. This process is important for maintaining cellular homeostasis and for degrading damaged organelles...&nbsp;&nbsp;<a href="/antibody-news/antibodies/p62-sqstm1-targeting-ubiquitinated-proteins-for-autophagic-degradation">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg12-a-ubiquitin-like-protein-essential-for-autophagosome-assembly">ATG12 - a ubiquitin-like protein essential for autophagosome assembly</a></strong><br/> <span>Atg12 is a ubiquitin-like protein that plays an essential role in cellular homeostasis by regulating the degradation and recycling of cytoplasmic organelles and macromolecules. Atg12 is one of two ubiquitin-like protein systems that is required du...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg12-a-ubiquitin-like-protein-essential-for-autophagosome-assembly">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/lc3-lc3b-measuring-autophagosome-formation-and-autophagic-flux">LC3/LC3B - measuring autophagosome formation and autophagic flux </a></strong><br/> <span>Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...&nbsp;&nbsp;<a href="/antibody-news/antibodies/lc3-lc3b-measuring-autophagosome-formation-and-autophagic-flux">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg9a-early-marker-autophagosome-assembly">ATG9A - early marker autophagosome assembly</a></strong><br/> <span>ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg9a-early-marker-autophagosome-assembly">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/comprehensive-autophagy-research-tools-new-catalog-available-now">Comprehensive Autophagy Research Tools - New Catalog Available Now!</a></strong><br/> <span>Autophagy, a protein degradation process through autophagosome-lysosomal pathway, is important for cellular homeostasis and plays a role in many diseases. To help researchers learn more about this process and the products available for its study, N...&nbsp;&nbsp;<a href="/antibody-news/antibodies/comprehensive-autophagy-research-tools-new-catalog-available-now">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/essential-to-death-atg5-autophagy-protein-5-apoptosis-specific-protein-asp">Essential to Death: ATG5 (autophagy protein 5, apoptosis-specific protein ASP)</a></strong><br/> <span>The ATG5 protein belongs to the ATG autophagy regulator family. This family controls the highly conserved cell's homeostatic response to a wide variety of both self- and foreign-originating cellular stimuli. ATG5 itself is ubiquitously expressed in mo...&nbsp;&nbsp;<a href="/antibody-news/antibodies/essential-to-death-atg5-autophagy-protein-5-apoptosis-specific-protein-asp">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg5-roles-in-cellular-defense">ATG5: Roles in Cellular Defense</a></strong><br/> <span>ATG5, or Autophagy Related 5, is a protein crucial for autophagy. Autophagy is a mechanism in which dysfunctional or pathogenic cells or cellular components are degraded and sometimes recycled. This process happens when ATG5 conjugates with another pr...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg5-roles-in-cellular-defense">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg5-autophagy-and-apoptosis">ATG5, Autophagy and Apoptosis</a></strong><br/> <span>ATG5 is a member of the ATG family that regulates autophagy, the evolutionary conserved homeostatic response to a diverse variety of self- and foreign-originating cellular stresses. ATG5 is ubiquitously expressed in cells and found co-localized with c...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg5-autophagy-and-apoptosis">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/atg5-from-autophagy-to-alzheimers-disease">ATG5: From Autophagy to Alzheimer's Disease</a></strong><br/> <span>Autophagy is a conserved mechanism whereby cells form double membrane autophagosomes to sequester cytoplasmic components for subsequent destruction by fusion with lysosomes (eukaryotes) or vacuoles (yeast). Targets of autophagy include aging proteins,...&nbsp;&nbsp;<a href="/antibody-news/antibodies/atg5-from-autophagy-to-alzheimers-disease">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/analyzing-lc3-in-western-blot">Analyzing LC3 in Western blot</a></strong><br/> <span>Microtubule-associated protein light chain 3 (LC3) is considered one of the definitive markers of autophagy, and its use is widespread in labs throughout the world. Despite its popularity, there are several considerations when employing LC3 antibodies...&nbsp;&nbsp;<a href="/antibody-news/antibodies/analyzing-lc3-in-western-blot">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap hidden"> <td> <strong><a href="/antibody-news/antibodies/the-role-of-lc3-within-the-autophagic-pathway">The Role of LC3 within the Autophagic Pathway</a></strong><br/> <span>We at Novus Biologicals have a broad antibody database covering the area of autophagy - over 1400 reagents in total. Autophagy is the bulk degradation of cytoplasmic components - literally, self-digestion of the cell. Double-membrane vesicles, called ...&nbsp;&nbsp;<a href="/antibody-news/antibodies/the-role-of-lc3-within-the-autophagic-pathway">Read full blog post.</a></span><br /><br /> </td> </tr></tbody> </table> </div><div class="ds_info"><span class="blogdiv">Showing 1-<span id="blogcnt">10</span> of 32 blog posts - <a class="blogs_showall">Show all blog posts</a>.</span></div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><div class="datasheet-ads-container" ><a href="https://www.bio-techne.com/resources/blogs/citation-widget-find-products-used-in-literature" target="_blank"><img loading="lazy" src="https://images.novusbio.com/design/citation-app-banner-349x119-v3.jpg" alt="Read our latest blog and use the new citation tool on bio-techne.com" title="new citation tool" class="datasheet-adzone" loading="lazy"></a></div><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div id="ss_prev_sidebar" class="cycle-prev ds_rp_prev"><div class="pager_left_on"></div></div><div id="ss_next_sidebar" class="cycle-next ds_rp_next"><div class="pager_right_on"></div></div><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="HAF008"> <div class="img_container"> <a href="/products/igg-antibody_haf008" title="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_HAF008_Western_Blot_19080.jpg" title="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-antibody_haf008">Goat anti-Rabbit IgG Secondary Antibody [HRP]</a> <br />HAF008</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-antibody_haf008';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="NB7160"> <div class="img_container"> <a href="/products/igg-h-l-antibody_nb7160" title="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 &amp; 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242)." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg" title="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 &amp; 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). 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Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242)." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-h-l-antibody_nb7160">Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]</a> <br />NB7160</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-h-l-antibody_nb7160';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="NBP2-24891"> <div class="img_container"> <a href="/products/igg-isotype-control_nbp2-24891" title="Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. 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Goat anti-rabbit FITC (Novus, 20302) was used as secondary." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-isotype-control_nbp2-24891">Rabbit IgG Isotype Control</a> <br />NBP2-24891</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-isotype-control_nbp2-24891';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> <div class="ds_info"> <div id="distributors"></div> </div> <div id="et_reload_trigger" style="display:none;">reload</div><h2 class="greyHead2">Product PDFs</h2><div class="silverHeadShort"> <span class="file"><a href="//resources.novusbio.com/protocols/Western-Blot-protocol-for-ATG5-Antibody-(NB110-53818).pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller">&nbsp;</span></a></span> <span class="download-file-text" ><a href="//resources.novusbio.com/protocols/Western-Blot-protocol-for-ATG5-Antibody-(NB110-53818).pdf" target="_blank" class="datasheet-download sdsFile">Western Blot Protocol</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//aero.bio-techne.com/sds/pdf/nb110-53818" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller">&nbsp;</span></a></span> <span class="download-file-text" ><a href="//aero.bio-techne.com/sds/pdf/nb110-53818" target="_blank" class="datasheet-download sdsFile">SDS</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//resources.novusbio.com/manual/Manual-NB110-53818-30877537.pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller">&nbsp;</span></a></span> <span class="download-file-text" ><a href="//resources.novusbio.com/manual/Manual-NB110-53818-30877537.pdf" target="_blank" class="datasheet-download sdsFile">Western Blot Protocol</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//www.novusbio.com/PDFs/NB110-53818.pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller">&nbsp;</span></a></span> <span class="download-file-text" ><a href="//www.novusbio.com/PDFs/NB110-53818.pdf" target="_blank" class="datasheet-download">PDF Datasheet</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="mailto:novus@novusbio.com?subject=Request%20CofA%20for%20NB110-53818" class="sprite_newDocument"><span class="downloadBlank">&nbsp;</span></a></span> <span class="download-file-text"><a href="mailto:nb-technical@bio-techne.com?subject=Request%20CofA%20for%20NB110-53818&body=Specific%20catalog%20number(if multiple sizes):%0ALot%20Number:%0AAny%20additional%20products:">Request CofA</a></span> </div><h2 class="greyHead2">Calculators</h2> <div class="calc_wrapper"> <div class="dsSprite_calc"></div> <div class="fake_link" id="calc_reconstitution_link">Concentration Calculator</div> <div id="calc_reconstitution_wrapper" class="calc_function_wrapper"> <div class="calc_popup"> <div class="calc_close"> <div class="dsSprite_calc_close"></div> </div> <div class="calc_header">Concentration Calculator</div> <div id="reconstitution_calculator" class="calculator"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. 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