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Human LILRB1/CD85j/ILT2 Antibody (MAB20172) by R&D Systems, Part of Bio-Techne

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View Human LILRB1/CD85j/ILT2 Antibody (MAB20172) validated in Human &amp; 3 applications from R&amp;D Systems." /> <script type="application/ld+json" id="json-ld">[{"@context":"http://schema.org","@type":"Product","additionalProperty":[{"additionalProperty":{"@type":"PropertyValue","name":"Applications","value":"Block/Neutralize, CyTOF-ready, Western Blot"}},{"additionalProperty":{"@type":"PropertyValue","name":"Immunogen","value":"Mouse myeloma cell line NS0-derived recombinant human LILRB1/CD85j/ILT2Gly24-His458Accession # Q8NHL6"}},{"additionalProperty":{"@type":"PropertyValue","name":"Specificity","value":"Detects human LILRB1/CD85j/ILT2 in direct ELISAs and Western blots. 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Products </li> <li> <a href="/t/lilrb1-cd85j-ilt2">LILRB1/CD85j/ILT2</a> </li> <li> <a href="/t/lilrb1-cd85j-ilt2/antibodies">LILRB1/CD85j/ILT2 Antibodies</a> </li> <li> Human LILRB1/CD85j/ILT2 Antibody (MAB20172) </li> </ol> </nav> </div> </div> </div> <div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <div id="product-page-details" data-template_in_use="antibody_template" data-time_total="0.81704306602478" data-time_to_compile="0.080039978027344" data-time_to_build="0.56436204910278" data-time_datapoints="0.17264103889465" data-time_template="-0.17264103889465" data-time_post_epcc="0.64440202713013" data-time_epcc="0.17264103889465" data-render_time="Friday 22nd of November 2024 10:28:18 AM" data-fallback="FALSE" data-time_to_process_data="0.13378000259399" class="px-4 container" class="container"> <div id="above-fold-section"> <div class="mb-2 o__product-summary"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Human LILRB1/CD85j/ILT2 Antibody </h1> <h2 class="font-weight-normal h4"> R&D Systems, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">MAB20172</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="reviews_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="reviews_quicklink"> <a href="#reviews" id="review_count_quicklink" class="reviews" data-tab="2"> <span class="review_stars review_stars_5"></span> <span class="font-weight-bold"></span> (1) </a> </div> </div> <div id="citation_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="citation_quicklink"> <a href="#citations" id="citation_count" data-tab="2" class="citations"> <span class="icon-icon-product-citations d-none d-lg-inline pr-1"></span> Citations (7)&nbsp; </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <div class="px-0 my-1" id="datasheet-summary-wrapper" data-section="section-base--top-jumplink"> <article class="top-jumplink-wrapper"> <span class="icon-icon-pdf text-error-red"></span> <a href="#product-documents" class="product-documents-top-link" rel="nofollow">Product Datasheet / COA / SDS</a> </article> </div> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" alt="R&amp;D Systems, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> <div class="row border-top mb-2" id="images-orderdetail-wrapper"> <div class="col-xl-6 mt-2" id="images-wrapper" data-section="image_summary"> <div id="image-tree" data-section="component-product-image-tree" class="vertical lightbox" data-mdb-zoom-effect="true"> <div id="product-image-gallery" class="row"> <div class="col-md-11 border rounded d-flex align-items-center justify-content-center product-image-lightbox"> <div class="text-center my-2" data-brand="rnd"> <img src="https://resources.bio-techne.com/images/products/antibody/ILT2_MAB20172_Block_Neutralize_16225.jpg" alt="Cell Adhesion Mediated by LILRB1/CD85j/ILT2 and Neutralization by Human LILRB1/CD85j/ILT2 Antibody." title="Cell Adhesion Mediated by LILRB1/CD85j/ILT2 and Neutralization by Human LILRB1/CD85j/ILT2 Antibody." data-caption="Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # &lt;a href=&quot;/p/proteins-enzymes/recombinant-human-lilrb1-cd85j-ilt2-fc-chimera-protein-cf_2017-t2&quot; target=&quot;_self&quot;&gt;2017-T2&lt;/a&gt;), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by acid phosphatase activity. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20172). The ND&lt;sub&gt;50&lt;/sub&gt; is typically 0.025-0.15 µg/mL." data-badges='' data-id="0" width="250" height="250" fetchpriority="high" as="image" class="search_result_thumbnail_image active" /> </div> </div> </div> </div> </div> <div class="col-xl-6 mt-2" id="orderdetails-wrapper" data-section="order_detail_block"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="MAB20172"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-3 p-0 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-2 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="MAB20172" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">MAB20172</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="MAB20172-SP" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">MAB20172-SP</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <div class="atc-control ProductCart-control text-right"> <div class="row container d-flex justify-content-end mx-0 mb-3 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="" class="atc-print-quote-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100">Print Quote</a> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MAB20172" class="atc-bulk-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> <div id="guarantee-statement" class="text-right" data-section="section-base--guarantee-statement"> <span class="icon-icon-check-circle"></span> <a href="/guarantee">100% Guarantee</a> </div> </div> </div> </div> </div> <div id="tabs-distributor" class="row"> <div class="tab-content col-xl-9 mt-2"> <div data-section="section-base--nav-tabs-content"> <ul class="nav nav-tabs nav-fill" id="productTab" role="tablist"> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor active" href="#tab-technical_data" id="technical_data-tab" data-toggle="tab" data-target="#technical_data" type="button" role="tab" aria-controls="technical_data" aria-selected="true"> <p class="m-0">Technical Data</p> </a> </li> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor " href="#tab-citations_reviews" id="citations_reviews-tab" data-toggle="tab" data-target="#citations_reviews" type="button" role="tab" aria-controls="citations_reviews" aria-selected="false"> <p class="m-0">Citations &amp; Reviews</p> </a> </li> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor " href="#tab-protocols_faqs" id="protocols_faqs-tab" data-toggle="tab" data-target="#protocols_faqs" type="button" role="tab" aria-controls="protocols_faqs" aria-selected="false"> <p class="m-0">Protocols</p> </a> </li> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor " href="#tab-calculators_tools" id="calculators_tools-tab" data-toggle="tab" data-target="#calculators_tools" type="button" role="tab" aria-controls="calculators_tools" aria-selected="false"> <p class="m-0">Calculators &amp; Tools</p> </a> </li> </ul> <div id="ProductTabContent" class="tab-content mb-3"> <div class="tab-pane fade show active" id="technical_data" role="tabpanel" aria-labelledby="technical_data-tab"> <div data-section="section-base--technical-anchors"> <ul class="product-pages-anchors nav mt-2"> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor active" href="#product-specifications"> Product Specifications</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#scientific-data"> Scientific Data</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#applications"> Applications</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#preparation--storage"> Preparation &amp; Storage</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#background"> Background</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#product-documents"> Product Documents</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#specific-notices"> Specific Notices</a> </li> </ul> </div> <div id="key_details" class="py-5 bt-pp-border-bottom" data-section="section-base--key-details"> <h3>Key Product Details</h3> <div class="mt-5 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Species Reactivity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <h4 class="d-block">Validated:</h4> <div class="d-block pb-2"> Human </div> <h4 class="d-block">Cited:</h4> <div class="d-block pb-2"> Human </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Applications</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <h4 class="d-block">Validated:</h4> <div class="d-block pb-2"> CyTOF-reported, Neutralization, Western Blot </div> <h4 class="d-block">Cited:</h4> <div class="d-block pb-2"> CyTof, Functional Assay, Neutralization </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Label</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Unconjugated </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Antibody Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal Mouse IgG<sub>2B</sub> Clone # 292319 </div> </div> </div> <div class="container"> <div class="row product-data"> <div class="col-lg-6 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4><a href="/t/lilrb1-cd85j-ilt2/antibodies">View all LILRB1/CD85j/ILT2 Antibodies »</a></h4> </div> </div> </div> </div> <div class="border-top border-bottom recommended-products-section" id="recommendation-viewed" data-product_code="MAB20172" data-type=viewed></div> <div id="product-specifications" class="bt-pp-border-bottom py-5" data-section="section-base--product-specifications"> <h3>Product Specifications</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Immunogen</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Mouse myeloma cell line NS0-derived recombinant human LILRB1/CD85j/ILT2<br />Gly24-His458<br />Accession # Q8NHL6 </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Specificity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Detects human LILRB1/CD85j/ILT2 in direct ELISAs and Western blots. In direct ELISAs,&nbsp;no cross-reactivity with recombinant human (rh)&nbsp;ILT1, 3, 4, 5, rhLIR-6 or rhLIR-8 is observed. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Clonality</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Host</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Mouse </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Isotype</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> IgG<sub>2B</sub> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Endotoxin Level</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <0.10 EU per 1 μg of the antibody by the LAL method. </div> </div> </div> </div> <div id="scientific-data" class="bt-pp-border-bottom py-5" data-anchor-label="Scientific Data" data-section="section-base--scientific-data"> <div> <h3>Scientific Data Images for Human LILRB1/CD85j/ILT2 Antibody </h3> </div> <div class="product-data-wrapper mt-5" id="scientific-data-table" data-scientific-count="-2"> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ILT2_MAB20172_Block_Neutralize_16225.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ILT2_MAB20172_Block_Neutralize_16225.jpg" alt="Cell Adhesion Mediated by LILRB1/CD85j/ILT2 and Neutralization by Human LILRB1/CD85j/ILT2 Antibody." title="Cell Adhesion Mediated by LILRB1/CD85j/ILT2 and Neutralization by Human LILRB1/CD85j/ILT2 Antibody." data-caption="Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # &lt;a href=&quot;/p/proteins-enzymes/recombinant-human-lilrb1-cd85j-ilt2-fc-chimera-protein-cf_2017-t2&quot; target=&quot;_self&quot;&gt;2017-T2&lt;/a&gt;), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by acid phosphatase activity. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20172). The ND&lt;sub&gt;50&lt;/sub&gt; is typically 0.025-0.15 µg/mL." data-badges="" data-application="Neutralization" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Cell Adhesion Mediated by LILRB1/CD85j/ILT2 and Neutralization by Human LILRB1/CD85j/ILT2 Antibody.</h4> Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # <a href="/p/proteins-enzymes/recombinant-human-lilrb1-cd85j-ilt2-fc-chimera-protein-cf_2017-t2" target="_self">2017-T2</a>), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by acid phosphatase activity. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20172). The ND<sub>50</sub> is typically 0.025-0.15 µg/mL. </div> </div> </div> <span class="product-data-wrapper table" id="more-scientific-data" style="display:none"> </span> </div> </div> <div id="applications" class="py-5 bt-pp-border-bottom" data-anchor-label="Applications" data-section="section-antibody--applications"> <h3>Applications for Human LILRB1/CD85j/ILT2 Antibody </h3> <div class="mt-5 py-2 container"> <div class="row"> <div class="col-lg-3 border font-weight-bold text-bg-dark py-2 align-top text-md-left text-white bg-dark-bt-blue d-none d-lg-block"> Application </div> <div class="col-lg-9 border align-top py-2 font-weight-bold text-white bg-dark-bt-blue d-none d-lg-block"> Recommended Usage </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>CyTOF-reported</h4> </div> <div class="col-lg-9 border align-top py-2"> Yabu, J.M.<em> et al</em>. (2016) PLoS ONE <b>11:</b>e0153355. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. </div> </div> <div class="row"> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Western Blot</h4> </div> <div class="col-lg-9 border align-top py-2"> 1 µg/mL <br/>Sample: Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2) </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Neutralization</h4> </div> <div class="col-lg-9 border align-top py-2"> Measured by its ability to neutralize LILRB1/CD85j/ILT2-mediated adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line. Cosman, D. <em>et al.</em> (1997) Immunity <strong>7</strong>:273. The Neutralization Dose (ND<sub>50</sub>) is typically 0.025-0.15&nbsp;µg/mL in the presence of&nbsp;10&nbsp;µg/mL Recombinant Human&nbsp;LILRB1/CD85j/ILT2 Fc Chimera. </div> </div> </div> <div class="product-data-wrapper mt-5"> Please Note: Optimal dilutions of this antibody should be experimentally determined. </div> <h4 class="mt-3">Reviewed Applications</h4> <p><span class="review_stars review_stars_5"></span> Read <a href="#reviews" class="reviews">1 review</a> rated 5 using MAB20172 in the following applications:</p> <ul class="list-unstyled"> <li><a href="#reviews" class="reviews" data-application="Western Blot">Western Blot (1 Review) </a></li> </ul> </div> <div id="preparation--storage" class="bt-pp-border-bottom py-5" data-section="section-base--preparation-storage"> <h3>Formulation, Preparation, and Storage</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Purification</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Protein A or G purified from hybridoma culture supernatant </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Reconstitution</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <div class="atc-container"> <div class="commerce-atc-reconstitution atc-block" data-type="reconstitution" data-blockid="ReconstitutionCart"> <div class="commerce-atc-reconstitution__instructions"> Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration. </div> <p class="atc-modal"></p> <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode=""> <div class="commerce-atc-reconstitution-header atc-header col-four row border-top pt-2 m-0"> <div class="commerce-atc-catnum atc-head col-md-6 font-weight-bold p-0">Reconstitution Buffer Available:</div> <div class="commerce-atc-price atc-head col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-md-3 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ReconstitutionCart" class="atc-lines"> <div class="commerce-atc-reconstitution-line productline col-four row py-2 m-0" data-catalog="RB01"> <div class="commerce-atc-title col-md-6 p-0"> <a href="">RB01: </a> </div> <div class="commerce-atc-price col-md-3"> </div> <div class="commerce-atc-quantity col-md-3 text-right p-0"> </div> </div> </div> <div class="atc-control ReconstitutionCart-control text-right mt-2"> <input type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc col-xs-4"/> </div> </div> </form> </div> </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Formulation</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Shipping</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Stability &amp; Storage</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL> </div> </div> </div> </div> <div id="background" class="py-5 bt-pp-border-bottom" data-section="section-base--background-info"> <h3>Background: LILRB1/CD85j/ILT2</h3> <article class="product-data-wrapper"> <p>The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1‑3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT-6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains, which allows association with Fc R gamma, an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1‑3). ILT2, also known as LIR1, MIR7, and CD85j, is expressed on most monocytes, dendritic cells, and mature B cells (1‑3). It is also expressed on small percentages of T cells and NK cells. ILT2 has four extracellular Ig-like domains and three cytoplasmic ITIMs. It functions as an inhibitory receptor that prevents cellular activation. ILT2 has been shown to bind classical (HLA-A and -B) and nonclassical (HLA-G1, -E and -F) MHC class I molecules (MHCI) (1‑3). ILT2 also binds with high affinity to an MHC class I homologue from human cytomegalovirus (3). Ligation of ILT2 by MHC class I may function to poise cellular activation thresholds and inhibit various leukocyte effector mechanisms that are regulated by MHC class I molecules on target cells. </p> </article> <div id="background_wrapper" class="px-3 mt-2"> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>References</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <OL> <LI>Allen, D. <EM>et al</EM>. (2000) Immunobiol. <STRONG>202</STRONG>:34.</LI> <LI>Colonna, M. <EM>et al</EM>. (1999) J. Leukocyte Biol. <STRONG>66</STRONG>:375.</LI> <LI>Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. <STRONG>11</STRONG>:209.</LI> <LI>Young, N. <EM>et al</EM>. (2001) Immunogenetics <STRONG>53</STRONG>:270.</LI></OL> </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Long Name</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Alternate Names</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> CD85j, ILT2, LIR1, MIR7 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Entrez Gene IDs</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.ncbi.nlm.nih.gov/gene?term=10859" class="a___link-external" target="_blank" rel="nofollow">10859</a> (Human) </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Gene Symbol</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> LILRB1 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>UniProt</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.uniprot.org/uniprot/?query=Q8NHL6" class="a___link-external" target="_blank" rel="nofollow">Q8NHL6</a> </div> </div> </div> <h4 class="mt-3">Additional LILRB1/CD85j/ILT2 Products</h4> <ul class="list-unstyled"> <li><a href="/t/lilrb1-cd85j-ilt2" data-product="LILRB1/CD85j/ILT2">All Products for LILRB1/CD85j/ILT2 </a></li> <li><a href="/t/lilrb1-cd85j-ilt2/cdna-clones" data-application="LILRB1/CD85j/ILT2 cDNA Clones">LILRB1/CD85j/ILT2 cDNA Clones </a></li> <li><a href="/t/lilrb1-cd85j-ilt2/lysates" data-application="LILRB1/CD85j/ILT2 Lysates">LILRB1/CD85j/ILT2 Lysates </a></li> <li><a href="/t/lilrb1-cd85j-ilt2/antibodies" data-application="LILRB1/CD85j/ILT2 Primary Antibodies">LILRB1/CD85j/ILT2 Primary Antibodies </a></li> <li><a href="/t/lilrb1-cd85j-ilt2/proteins-enzymes" data-application="LILRB1/CD85j/ILT2 Proteins and Enzymes">LILRB1/CD85j/ILT2 Proteins and Enzymes </a></li> </ul> </div> <div id="product-documents" data-section="section-base--product-documents"> <div id="accordion" class="border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3"> Product Documents for Human LILRB1/CD85j/ILT2 Antibody </h3> </div> <div class="vtab-section" data-section="Product Datasheets"> <div class="card-header rounded-top"> <a class="" data-toggle="collapse" data-target="#collapse_two" aria-expanded="true" aria-controls="collapse_two" id="product-datasheets-link"> <h5 class="mb-0"> Product Datasheets </h5> </a> </div> <div id="collapse_two" class="collapse show prod_datasheet_link" aria-labelledby="heading_two" 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