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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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<div id="container-ec98e75259" class="cmp-container"> <div class="cmp-p-container p-l1 mt-l1"> <div id="container-d0c7a351e0" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-title aem-GridColumn aem-GridColumn--default--12"><div id="title-5b0ceb8eaa" class="cmp-title"> <h3 class="cmp-title__text">The proof of the performance is in the data</h3> </div> </div> <div class="cmp-p-text mt-l1 mb-l1 alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1562736599" class="cmp-text"> <p>Clear, detailed publication-quality images and quantitative data are what the CellInsight High-Content Screening platforms are engineered for. Review <a href="#sample-applications">sample applications</a>, including fluorescence images, graphs, and videos for life sciences applications such as angiogenesis, apoptosis, autophagy, cell cycle and proliferation, endocytosis, and viability. Browse our <a href="#instrument-antibodies-assays">antibodies and assays</a>. Do not forget to check out our <a href="#image-gallery">image gallery</a> below. </p> </div> </div> </div> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> <div class="cmp-p-sectioncontainer p-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-33eed63266"> <div class="cmp-sectiontitle"> <h2 id="sample-applications" class="cmp-sectiontitle__text" data-anchor-nav-title="Sample applications">Sample applications</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-172a0eb644" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1146639766" class="cmp-text"> <p>The Thermo Scientific CellInsight high-content platform allows you to take advantage of the entire fluorescent spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions. To that end, we have a myriad of antibodies and assays compatible with the CellInsight systems. Below is a comprehensive list of applications using assays together with the high-content platform.</p> </div> </div> </div> </div> </div> <div class="cmp-p-container m--no-gutters p-l1 mt-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-a75fc017bb" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-accordion aem-GridColumn aem-GridColumn--default--12"><div id="accordion-c0d13fbb1c" class="cmp-accordion" data-cmp-is="accordion"> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-a378452779"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-a378452779-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-a378452779-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS angiogenesis assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-a378452779-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-a378452779-button"><div class="cmp-p-container"> <div id="container-a378452779" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-8e6c8767d2" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-98964947" class="cmp-text"> <p>This reliable, automated assay identifies angiogenic tubes (micro-capillaries) formed by endothelial cells and provides quantitative measurements related to angiogenic tube formation such as the number of tubes, their morphology and branching, and an angiogenic index.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>% connected tubes</li> <li>Mean connected tube area</li> <li>Mean connected width</li> <li>Mean connected tube node spacing</li> <li>Angiogenic index</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield or confocal</li> <li>5x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/cytoskeleton.html" data-uw-rm-brl="false">Cytoskeleton stains</a><u></u></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/cell-tracking.html" data-uw-rm-brl="false">CellMask stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-0b41376580" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"> <div id="container-f86a6529e2" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 "> <div class="cmp-p-container p-l1 m--learning-center-container-border aem-GridColumn aem-GridColumn--default--10"> <div id="container-145d84f6f2" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-angiogenesis-image.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-angiogenesis-image.jpg" id="image-482265186" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-angiogenesis-image.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt/> </noscript> </div>  </div> <div class="cmp-p-text mt-l1"> <div id="text-261216988" class="cmp-text"> <p><b>HUVEC cells, grown in Matrigel support and treated with angiogenic compounds and imaged at 5x magnification. </b></p> <p> </p> <p><b>(Left)</b> Cell nuclei are stained with Invitrogen Molecular Probes <a href="/order/catalog/product/H3570" data-uw-rm-brl="false">Hoechst 33342</a> (shown in red) and the cytoskeleton (actin fibers) is stained using a phalloidin conjugate (shown in green/yellow; <a href="/us/en/home/life-science/cell-analysis/cell-structure/cytoskeleton.html" data-uw-rm-brl="false">wide choice of labels</a>). <b>(Right)</b> Angiogenesis features identified using Thermo Scientific HCS Studio Software. In a two-channel assay, connected tubes are automatically identified using a blue overlay, and branching nodes are displayed as pink spots. Multiple parameters can be automatically measured in additional detection channels and correlated with the identified tube structures.</p> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"> <div id="container-8ec8546d3f" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 "> <div class="cmp-p-textimage aem-GridColumn aem-GridColumn--default--10"><div class="cmp-textimage cta-container" id="textimage-806769002" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title">Suramin serves as a potent inhibitor of angiogenesis.</h2> <p>A dose-response for suramin concentration can be plotted against a variety of properties measured automatically using Thermo Scientific HCS platform with Thermo Scientific HCS Studio software and plotted using GraphPad Prism software.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-angiogenesis-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-angiogenesis-dose-response.jpg" data-title="Suramin serves as a potent inhibitor of angiogenesis " id="textimage-806769002" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-angiogenesis-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph of five different angiogenesis assay parameters" title="Suramin serves as a potent inhibitor of angiogenesis "/> </noscript> <meta itemprop="caption" content="Suramin serves as a potent inhibitor of angiogenesis "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-ce382ddc90"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-ce382ddc90-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-ce382ddc90-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS apoptosis—caspase assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-ce382ddc90-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-ce382ddc90-button"><div class="cmp-p-container"> <div id="container-ce382ddc90" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-418fbfd088" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="text-1268914030" class="cmp-text"> <p>Caspase assay for HCS that allows the simple detection of active caspases in living cells in real time or in fixed cells.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Fluorescence intensity, morphology, and count values of spots in different cell regions</li> <li>Average fluorescence intensity difference and ratios between different regions for each cell</li> <li>Intensity and count ratio between channels within different regions for each cell</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield or confocal</li> <li>10x or 20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/imaging-apoptosis-assays/imaging-caspase-activity.html" data-uw-rm-brl="false">Caspase assay kits</a><u></u></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"> <div id="container-eeec5aae73" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 "> <div class="cmp-p-textimage m--teaser aem-GridColumn aem-GridColumn--default--10"><div class="cmp-textimage cta-container" id="textimage-509805643" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><strong>Hap1 cells were labeled with <a href="/order/catalog/product/C10423">CellEvent Caspase-3/7 Green reagent</a> and <a href="/order/catalog/product/H1399">Hoechst 33342</a> following treatment with staurosporine.</strong> The cells were imaged using a <a href="/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/platforms/cx5.html">Thermo Scientific CellInsight CX5 high-content platform</a> <strong>(row A)</strong> and <a href="/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html">Thermo Scientific HCS Studio Software</a> , which were used to automatically identify <strong>(row B)</strong> and quantify the intensity of CellEvent green fluorescence from each cell.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis.jpg" data-title="Hap1 cells " id="textimage-509805643" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="HCS Apoptosis Caspase Assay" title="Hap1 cells "/> </noscript> <meta itemprop="caption" content="Hap1 cells "/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"> <div id="container-0d0c169cca" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 "> <div class="cmp-p-textimage aem-GridColumn aem-GridColumn--default--10"><div class="cmp-textimage cta-container" id="textimage-78120038" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-dependent increase in the induction of apoptosis, as reported by activation of Caspase 3/7.</b> The sensitivity of two cell types: wild type Hap1 (red) and Hap1 lacking ATG5 (blue), were compared.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-data.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-data.jpg" data-title="Dose-dependent increase in the induction of apoptosis" id="textimage-78120038" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-data.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Line graph of mean signal intensity vs staurosporine concentration" title="Dose-dependent increase in the induction of apoptosis"/> </noscript> <meta itemprop="caption" content="Dose-dependent increase in the induction of apoptosis"/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-f3db2d0eba"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-f3db2d0eba-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-f3db2d0eba-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS Apoptosis—TUNEL Assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-f3db2d0eba-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-f3db2d0eba-button"><div class="cmp-p-container"> <div id="container-f3db2d0eba" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-f4eed7ed32" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-2006146377" class="cmp-text"> <p>This assay uses a nuclear stain to identify cells and a TUNEL label to measure DNA strand breaks. The Invitrogen Click-iT TUNEL Alexa Fluor Imaging Assay kits are fast and efficient and offer precise, quantitative data even with high levels of apoptotic cells. The kits are optimized for HCS and provide a choice of three wavelength options to aid in multiplexing with other cellular measurements.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Fluorescence intensity, morphology, and count values for each object</li> <li>Fluorescence intensity, morphology, and count values of spots in different cell regions</li> <li>Average fluorescence intensity difference and ratios between different regions for each cell</li> <li>Intensity and count ratio between channels within different regions for each cell</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield or confocal</li> <li>20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stain</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/imaging-apoptosis-assays/tunel-assays-dna-fragmentation-chromatin-condensation.html" data-uw-rm-brl="false">TUNEL assay kits</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"> <div id="container-e93089b8e9" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"><div class="cmp-textimage cta-container" id="textimage-1622276070" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>HeLa cells treated to induce apoptosis and imaged.</b> Untreated <b>(Left)</b> and staurosporine-treated <b>(Right)</b> HeLa cells stained using the Invitrogen Molecular Probes <a href="/order/catalog/product/C10246">Click-iT TUNEL Alexa Fluor 594 Imaging Assay, for Microscopy & HCS</a> and Invitrogen Molecular Probes <a href="/order/catalog/product/H3570">Hoechst 33342</a>. Cells were imaged using a Thermo Scientific HCS instrument, and fluorescence intensities and count ratios were measured using Thermo Scientific HCS Studio Software.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-tunel-image1.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-tunel-image1.jpg" data-title="HeLa cells treated to induce apoptosis and imaged" id="textimage-1622276070" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-tunel-image1.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Two-panel HCS image of nonapoptotic and apoptotic cells" title="HeLa cells treated to induce apoptosis and imaged"/> </noscript> <meta itemprop="caption" content="HeLa cells treated to induce apoptosis and imaged"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"> <div id="container-46a2fbe78f" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"><div class="cmp-textimage cta-container" id="textimage-674174032" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plots for a variety of compounds and cell types using Invitrogen Molecular Probes Click-iT TUNEL Alexa Fluor 647 to measure apoptosis. </b>Responses were measured automatically using Thermo Scientific HCS platform with Thermo Scientific HCS Studio 2.0 Cell Analysis Software and plotted using GraphPad Prism software.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-dose-response.jpg" data-title="Dose-response plots for a variety of compounds and cell types " id="textimage-674174032" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Four-panel graph of data for different angiogenesis assays" title="Dose-response plots for a variety of compounds and cell types "/> </noscript> <meta itemprop="caption" content="Dose-response plots for a variety of compounds and cell types "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-84b00fff00"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-84b00fff00-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-84b00fff00-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS autophagy assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-84b00fff00-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-84b00fff00-button"><div class="cmp-p-container"> <div id="container-84b00fff00" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-d6e8e00b69" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-466512831" class="cmp-text"> <p>In this assay, quantitation of LC3B protein on autophagic vesicles is achieved using a fixed end-point assay based on immunofluorescence detection. Cells are identified using a nuclear stain and LC3B expression associated with each cell is measured.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Fluorescence intensity, morphology, and count values for each object</li> <li>Fluorescence intensity, morphology, and count values of spots in different cell regions</li> <li>Average fluorescence intensity difference and ratios between different regions for each cell</li> <li>Intensity and count ratio between channels within different regions for each cell</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield</li> <li>20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stain</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/autophagy/autophagy-lc3b-p62.html#prd1" data-uw-rm-brl="false">LC3B detection</a></li> <li><a href="/sg/en/home/life-science/antibodies/secondary-antibodies.html" data-uw-rm-brl="false">Secondary antibodies</a><u></u></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/cell-tracking.html" data-uw-rm-brl="false">Cell demarcation</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"> <div id="container-dd3629a15c" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--10"> <div id="container-e053608196" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-image.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-image.jpg" id="image-695030516" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-image.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt/> </noscript> </div>  </div> <div class="cmp-p-container"> <div id="container-88b85c1dff" class="cmp-container"> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-22741074" class="cmp-text"> <p><b>A549 cells treated to induce autophagy and imaged.(Left)</b> A549 cells were treated with chloroquine and stained with Invitrogen <a href="/order/catalog/product/H3570" data-uw-rm-brl="false">Hoechst 33342</a>, Invitrogen <a href="/order/catalog/product/H32721" data-uw-rm-brl="false">HCS CellMask Deep Red</a>, and an anti-LC3B with Invitrogen Alexa Fluor 488 goat anti-rabbit antibody. Cells accumulate LC3B at higher chloroquine concentrations. <b>(Right)</b> Using Thermo Scientific CellInsight CX5 high-content platform and Thermo Scientific HCS Studio Software, autophagy features were automatically identified: nuclei (blue), cells (green boundary), and LC3B was assayed both by granule count (pink) and by measuring fluorescence intensity in the 488 channel.</p> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-b103939833" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--default--0"> <div id="container-09326237b7" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--5 aem-GridColumn--offset--default--0"> <div id="container-a14d8ebf93" class="cmp-container"> <div class="aem-Grid aem-Grid--default--5 "> <div class="cmp-p-textimage m--teaser aem-GridColumn aem-GridColumn--default--5"><div class="cmp-textimage cta-container" id="textimage-1785042028" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plot of LC3B granules per cell with increasing chloroquine concentration.</b></p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-dose-response-granules.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-dose-response-granules.jpg" data-title="Dose-response plot of LC3B granules per cell" id="textimage-1785042028" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-dose-response-granules.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph of granules vs chloroquine concentration " title="Dose-response plot of LC3B granules per cell"/> </noscript> <meta itemprop="caption" content="Dose-response plot of LC3B granules per cell"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--5 aem-GridColumn--offset--default--0"> <div id="container-c15c1b3aed" class="cmp-container"> <div class="aem-Grid aem-Grid--default--5 "> <div class="cmp-p-textimage m--teaser aem-GridColumn aem-GridColumn--default--5"><div class="cmp-textimage cta-container" id="textimage-1687019904" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plot of LC3B fluorescence intensity with increasing chloroquine concentration.</b></p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-dose-response-fluorescence.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-dose-response-fluorescence.jpg" data-title="Dose-response plot of LC3B fluorescence intensity " id="textimage-1687019904" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-autophagy-dose-response-fluorescence.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph of fluorescence vs chloroquine concentration" title="Dose-response plot of LC3B fluorescence intensity "/> </noscript> <meta itemprop="caption" content="Dose-response plot of LC3B fluorescence intensity "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-360a309f04"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-360a309f04-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-360a309f04-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS cell cycle assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-360a309f04-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-360a309f04-button"><div class="cmp-p-container"> <div id="container-360a309f04" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-f8bc9c66e8" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1233795692" class="cmp-text"> <p>Cell cycle assays and mitotic index measurements provide insight into cell division and quantify the effects of compounds or treatments that impact mitotic progression. Automated assays can measure cellular DNA content as an indicator of where in the cycle a cell was stained, or you can detect the levels of proteins associated with mitosis such as Histone H3.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>DNA content</li> <li>Intensity levels of up to 3 secondary targets</li> <li>Correlation at single-cell and population level</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield</li> <li>10x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/cell-tracking.html" data-uw-rm-brl="false">Whole-cell stains</a></li> <li><a href="/sg/en/home/life-science/antibodies/secondary-antibodies.html" data-uw-rm-brl="false">Secondary antibodies</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-17fa893451" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-693633226" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Cell cycle assay. </b>A549 cells were treated with increasing doses of a mitotic inhibitor for 24 hours. The cells were stained for phospho Histone H3 and detected using an Invitrogen Alexa Fluor 488 secondary antibody. Invitrogen <a href="/order/catalog/product/H10294">HCS NuclearMask Deep Red stain</a> was used as a nuclear segmentation tool.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cell-cycle-image1.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cell-cycle-image1.jpg" data-title="Cell cycle assay for A549 cells" id="textimage-693633226" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cell-cycle-image1.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Two-panel HCS image showing cell cycle assay results on control vs nocodazole treated cells " title="Cell cycle assay for A549 cells"/> </noscript> <meta itemprop="caption" content="Cell cycle assay for A549 cells"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-aeb96a7fc8" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-559264511" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plot of mitotic inhibitors in A549 cells using a non-linear regression with GraphPad Prism software to determine an EC</b><a href="http://www.i2symbol.com/symbols/subscript" style="font-weight: bold;background-color: rgb(255,255,255);">₅</a><a href="http://www.i2symbol.com/symbols/subscript" style="font-weight: bold;background-color: rgb(255,255,255);">₀</a><b style="">.</b></p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cell-cycle-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cell-cycle-dose-response.jpg" data-title="Dose-response plot of mitotic inhibitors in A549 cells " id="textimage-559264511" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cell-cycle-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Two-panel graph showing effect of different mitotic inhibitors " title="Dose-response plot of mitotic inhibitors in A549 cells "/> </noscript> <meta itemprop="caption" content="Dose-response plot of mitotic inhibitors in A549 cells "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-e00e0438cd"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-e00e0438cd-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-e00e0438cd-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS cell proliferation assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-e00e0438cd-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-e00e0438cd-button"><div class="cmp-p-container"> <div id="container-e00e0438cd" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-e037096443" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1365788481" class="cmp-text"> <p>This automated assay measures new DNA synthesis based on incorporation of the nucleoside analog EdU into DNA. A copper-catalyzed “click” reaction adds a fluorescent Invitrogen Alexa Fluor dye conjugate to the EdU and enables detection with a range of wavelength choices for easy multiplexing.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>DNA content</li> <li>Intensity levels of up to 3 secondary targets</li> <li>Correlation at single-cell and population level</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>Total cells</li> <li>Replicating cells</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a><br /> </li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/cell-proliferation/click-it-edu-assays.html" data-uw-rm-brl="false">EdU detection using Alex Fluor dye</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-8ad949b816" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-531189849" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>A549 cell proliferation assay. </b>A549 cells were treated with increasing doses of paclitaxel. Cells were stained with Invitrogen <a href="/order/catalog/product/C10640">Click-iT Plus EdU Alexa Fluor 647 Imaging Kit</a> (red) and Invitrogen <a href="/order/catalog/product/H3570">Hoechst 33342 stain</a> (blue). Cells were imaged using the Thermo Scientific CellInsight CX7 High-Content Analysis Platform, and total cells and replicating cells were measured using Thermo Scientific HCS Studio Software.</p> <p> </p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-proliferation-image1.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-proliferation-image1.jpg" data-title="A549 cell proliferation assay" id="textimage-531189849" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-proliferation-image1.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Multipanel HCS images showing the effect of paclitaxel on proliferation" title="A549 cell proliferation assay"/> </noscript> <meta itemprop="caption" content="A549 cell proliferation assay"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-0c8b03427e" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-1824987074" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plots of U2OS cells treated with increasing concentrations of proliferation inhibitors and stained using Invitrogen</b> <b>Molecular Probes Click-iT Plus EdU labeled with Invitrogen Molecular Probes Alexa Fluor 647 dye.</b></p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-proliferation-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-proliferation-dose-response.jpg" data-title="Dose-response plots of U2OS cells" id="textimage-1824987074" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-proliferation-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Two-panel graph showing proliferation vs increasing inhibitor concentration " title="Dose-response plots of U2OS cells"/> </noscript> <meta itemprop="caption" content="Dose-response plots of U2OS cells"/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-80bbe19d00"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-80bbe19d00-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-80bbe19d00-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS cytoskeletal rearrangement assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-80bbe19d00-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-80bbe19d00-button"><div class="cmp-p-container"> <div id="container-80bbe19d00" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-25263808d6" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-517327115" class="cmp-text"> <p>This assay automatically measures changes in cell morphology reflecting changes in the underlying intracellular structure. Specifically, the assay measures the morphology of the whole cell and the nucleus, as well as the number, dimensions, intracellular location, and arrangement of F-actin and microtubule fibers.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Whole cell shape and dimensions</li> <li>Number of cytoskeletal fibers, their dimensions, and alignment</li> <li>Intracellular arrangement, location, and texture measurements of cytoskeletal labels</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms- second image was imaged using the CX7 platform</li> <li>Widefield, 20x or 40x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/cell-tracking.html" data-uw-rm-brl="false">Whole-cell stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/cytoskeleton.html" data-uw-rm-brl="false">Cytoskeleton stains</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-909d76a7bb" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"> <div id="container-7dfb5cf83e" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cytoskeleton-fig1.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cytoskeleton-fig1.jpg" id="image-1001538819" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cytoskeleton-fig1.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt/> </noscript> </div>  </div> <div class="cmp-p-container"> <div id="container-9f0f77191d" class="cmp-container"> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-2110196951" class="cmp-text"> <p><b>Cytoskeletal rearrangement assay. </b>A549 cells were treated with cytochalasin-D and analyzed using the Thermo Scientific CellInsight CX7 High-Content Analysis Platform. Nuclei are labeled with Invitrogen <a href="/order/catalog/product/H10325">HCS NuclearMask Blue Stain</a> and F-actin is labeled with Invitrogen <a href="/order/catalog/product/A12379">Alexa Fluor 488 phalloidin</a>, and the whole cell is demarcated using Invitrogen <a href="/order/catalog/product/H32721">HCS CellMask Deep Red Stain</a>. The assay automatically segments the cells (yellow overlay) and identifies the location and orientation of actin fibers (green overlay). Actin fibers greater than a threshold length are identified and labeled (red overlay). Individual cells can be identified from bar charts and scatter plots.</p> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-8ad23577b6" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-1641255629" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Cellular dose-response to cytochalasin as shown by cell area, nuclear intensity, actin fiber count, tubulin fiber count, and actin fiber intensity.</b></p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cytoskeleton-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cytoskeleton-dose-response.jpg" data-title="Cellular dose-response to cytochalasin " id="textimage-1641255629" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-cytoskeleton-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph of five different cytoskeletal rearrangement assay parameters" title="Cellular dose-response to cytochalasin "/> </noscript> <meta itemprop="caption" content="Cellular dose-response to cytochalasin "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-77b89bd31d"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-77b89bd31d-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-77b89bd31d-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS DNA damage assays</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-77b89bd31d-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-77b89bd31d-button"><div class="cmp-p-container"> <div id="container-77b89bd31d" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-98ca1e8dd8" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-2078479879" class="cmp-text"> <p>The HCS DNA Damage Kit uses a secondary antibody conjugate to detect phosphorylated H2AX, Image-iT DEAD Green dye to detect cytotoxicity, and Hoechst 33342 to label nuclei in both live and dead cells.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Whole cell shape and dimensions</li> <li>Number of cytoskeletal fibers, their dimensions, and alignment</li> <li>Intracellular arrangement, location, and texture measurements of cytoskeletal labels</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>DNA damage</li> <li>Cytotoxicity</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/order/catalog/product/H10292" data-uw-rm-brl="false">HCS DNA Damage Kit</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-08706c397b" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-446719286" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Using the <a href="/order/catalog/product/H10292">HCS DNA Damage Kit</a>, researchers can detect and quantitate DNA damage and changes in cell permeability.</b></p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/data-2.par.18541.image.275.225.1.dat-hcs-dna-damage-s006328-jpg.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/data-2.par.18541.image.275.225.1.dat-hcs-dna-damage-s006328-jpg.jpg" data-title="DNA damage and changes in cell permeability" id="textimage-446719286" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/data-2.par.18541.image.275.225.1.dat-hcs-dna-damage-s006328-jpg.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graphical representation of the DNA damage assay experiment" title="DNA damage and changes in cell permeability"/> </noscript> <meta itemprop="caption" content="DNA damage and changes in cell permeability"/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-66a82efa2e"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-66a82efa2e-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-66a82efa2e-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS endocytosis assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-66a82efa2e-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-66a82efa2e-button"><div class="cmp-p-container"> <div id="container-66a82efa2e" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-48ef73bec0" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-145643838" class="cmp-text"> <p>Endocytosis is involved in many cellular processes such as nutrient acquisition and receptor signaling. Analysis of the extent of internalization of a given molecule can be used as a marker of endocytosis. Commonly used markers include ligands such as LDL, EGF, or transferrin as well as fluid phase markers, for example 10,000 MW dextrans conjugated to fluorophores to determine their localization.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Fluorescence intensity, morphology, and count values for each object</li> <li>Fluorescence intensity, morphology, and count values of spots in different cell regions</li> <li>Average fluorescence intensity difference and ratios between different regions for each cell</li> <li>Intensity and count ratio between channels within different regions for each cell</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms- second image was imaged using the CX5 platform</li> <li>Widefield or confocal</li> <li>20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/endocytosis-and-pinocytosis.html" data-uw-rm-brl="false">Dextran conjugates</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/antibody-internalization.html" data-uw-rm-brl="false">Antibody internalization</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/ligand-internalization.html" data-uw-rm-brl="false">Ligand internalization</a></li> <li><a href="/sg/en/home/brands/molecular-probes/key-molecular-probes-products/phrodo-indicators.html" data-uw-rm-brl="false">pH rodo indicators</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-e63e6a3df3" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"> <div id="container-3b8e44f2c9" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-endocytosis.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-endocytosis.jpg" id="image-205640890" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-endocytosis.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt/> </noscript> </div>  </div> <div class="cmp-p-container"> <div id="container-e0d8d0f635" class="cmp-container"> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-1807428160" class="cmp-text"> <p><strong>U2OS cells stably expressing a GFP-EGF receptor construct and incubated with <a href="/order/catalog/product/P35374">pHrodo red EGF conjugate</a> were treated with PitStop 2 inhibitor (Abcam, Inc.) to inhibit endocytosis.</strong> Cells were stained with Invitrogen <a href="/order/catalog/product/H1399">Hoechst 33342</a>, and <a href="/order/catalog/product/P35374">pHrodo red EGF conjugate</a>. Stained cells were imaged using <a href="/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/platforms/cx5.html">Thermo Scientific CellInsight CX5 high-content platform</a> (row A) and analyzed using <a href="/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html">HCS Studio Software</a>. Internalized EGF receptors and the EGF ligand were automatically identified (row B): nuclei (blue), cells (green boundary), and EGF or the EGF receptor were assayed both by granule count (red for pHrodo EGF or green for GFP-EGF receptor).</p> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-efc7afd40e" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-102584010" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-dependent loss of EGF receptor (green trace) and EGF internalization (red trace) with increasing concentrations of Pitstop 2 inhibitor (Abcam, Inc.) in U2OS cells.</b> At high concentrations of Pitstop 2 inhibitor, cells are unable to internalize the EGF receptor and its ligand.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-endocytosis-data.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-endocytosis-data.jpg" data-title="Dose-dependent loss of EGF receptor/EGF " id="textimage-102584010" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-endocytosis-data.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="HCS Endocytosis Assay sample data" title="Dose-dependent loss of EGF receptor/EGF "/> </noscript> <meta itemprop="caption" content="Dose-dependent loss of EGF receptor/EGF "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-e080b4f436"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-e080b4f436-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-e080b4f436-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS lipotoxicity assays</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-e080b4f436-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-e080b4f436-button"><div class="cmp-p-container"> <div id="container-e080b4f436" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-a051f9d930" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1640689013" class="cmp-text"> <p>HCS LipidTOX neutral lipid stains were developed for image-based high-content screening (HCS) assays to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX assay series includes neutral lipid stains for fixed cells and phospholipid stains for live cells, which can be combined in multiplexed assays.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Fluorescence intensity, morphology, and count values of spots in different cell regions</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><i>See selection guides below</i></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-0b3f4346d9" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-1622797971" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Lipid vesicles in bone marrow—derived hMSCs visualized with <a href="/order/catalog/product/H34475">LipidTOX Green neutral lipid stain</a> and counterstained with Hoechst 33342</b></p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/data-2.par.35192.image.250.148.1.dat-lipidtoxicity-s005510-jpg.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/data-2.par.35192.image.250.148.1.dat-lipidtoxicity-s005510-jpg.jpg" data-title="Lipid vesicles in bone marrow—derived hMSCs" id="textimage-1622797971" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/data-2.par.35192.image.250.148.1.dat-lipidtoxicity-s005510-jpg.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Microscopic view of HCS Lipidtox assay results showing staining of neutral lipid stains and phospholipids " title="Lipid vesicles in bone marrow—derived hMSCs"/> </noscript> <meta itemprop="caption" content="Lipid vesicles in bone marrow—derived hMSCs"/> </div>  </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-98fb30bf14" class="cmp-container"> <div class="cmp-p-title"><div id="title-89f640ca50" class="cmp-title"> <h3 class="cmp-title__text">Steatosis/Adipogenesis</h3> </div> </div> <div class="cmp-p-text"> <table class="rte-table"> <tbody><tr><td style="border: none;"> </td> <th><a href="/order/catalog/product/H34477">HCS LipidTOX Deep Red Neutral Lipid Stain, for cellular imaging</a></th> <th><a href="/order/catalog/product/H34476">HCS LipidTOX Red Neutral Lipid Stain, for cellular imaging</a></th> <th><a href="/order/catalog/product/H34475">HCS LipidTOX Green Neutral Lipid Stain, for cellular imaging</a></th> </tr><tr><td><b>Readout</b></td> <td colspan="3" style="text-align: center;">High affinity for neutral lipid droplets in fixed cells</td> </tr><tr><td><b>Common filter set</b></td> <td style="background-color: rgb(255, 0, 0); color: rgb(255, 255, 255); text-align: center;" align="center">Cy5</td> <td style="background-color: rgb(255, 57, 0); color: rgb(255, 255, 255); text-align: center;" align="center">Texas Red</td> <td style="background-color: rgb(0, 150, 97); color: rgb(255, 255, 255); text-align: center;" align="center">FITC</td> </tr><tr><td><b>Reporter</b></td> <td style="background-color: rgb(255, 0, 0); color: rgb(255, 255, 255); text-align: center;" align="center">LipidTOX Deep Red neutral lipid stain</td> <td style="background-color: rgb(255, 57, 0); color: rgb(255, 255, 255); text-align: center;" align="center">LipidTOX Red neutral lipid stain</td> <td style="background-color: rgb(0, 150, 97); color: rgb(255, 255, 255); text-align: center;" align="center">LipidTOX Green neutral lipid stain</td> </tr><tr><td><b>Ex/Em (nm)</b></td> <td style="background-color: rgb(255, 0, 0); color: rgb(255, 255, 255); text-align: center;" align="center">637/655</td> <td style="background-color: rgb(255, 57, 0); color: rgb(255, 255, 255); text-align: center;" align="center">577/609</td> <td style="background-color: #009661; color: #fff;" align="center">495/505</td> </tr><tr><td><b>Live-cell compatible</b></td> <td colspan="3" align="center" style="text-align: center;">No</td> </tr><tr><td><b>Added to growth media</b></td> <td colspan="3" align="center" style="text-align: center;">No</td> </tr><tr><td><b>Formaldehyde fixable</b></td> <td colspan="3" style="text-align: center;">Cells labeled after fixation</td> </tr><tr><td><b>Multiplexing</b></td> <td colspan="3" style="text-align: center;">Can be multiplexed with phospholipidosis detection reagents (see tables below)</td> </tr><tr><td><b>Platform</b></td> <td colspan="3" align="center" style="text-align: center;">Imaging, HCS</td> </tr><tr><td><b>Bibliography</b></td> <td colspan="3" align="center" style="text-align: center;"><a href="/search/supportSearch?query=cytotoxicity%2C+imaging%2C+lipid&type=Citations+%26+References" class="headline">Citations</a></td> </tr><tr><td><b>Format</b></td> <td colspan="3" style="text-align: center;">125 μL (1,200 assays for steatosis/240 assays for adipogenesis)</td> </tr><tr><td><b>Cat. No. </b></td> <td align="center" style="text-align: center;"><a href="/order/catalog/product/H34477">H34477</a></td> <td align="center" style="text-align: center;"><a href="/order/catalog/product/H34476">H34476</a></td> <td align="center" style="text-align: center;"><a href="/order/catalog/product/H34475">H34475</a></td> </tr></tbody></table> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-efa0a2599b" class="cmp-container"> <div class="cmp-p-title"><div id="title-8310d3e9d6" class="cmp-title"> <h3 class="cmp-title__text">Steatosis/Phospholipidosis</h3> </div> </div> <div class="cmp-p-text"> <table class="rte-table"> <tbody><tr><td style="border: none;"> </td> <th><a href="/order/catalog/product/H34158">HCS LipidTOX Phospholipidosis and Steatosis Detection Kit</a></th> <th> </th> </tr><tr><td><b>Readout</b></td> <td colspan="2" style="text-align: center;">Kit for the sequential analysis of phospholipidosis and steatosis</td> </tr><tr><td><b>Common filter set</b></td> </tr><tr><td><b>Reporter</b></td> <td style="background-color: rgb(0, 150, 97); color: rgb(255, 255, 255); text-align: center;" align="center">LipidTOX Green neutral lipid stain</td> <td> </td> </tr><tr><td><b>Ex/Em (nm)</b></td> <td style="background-color: rgb(0, 150, 97); color: rgb(255, 255, 255); text-align: center;" align="center">495/505</td> <td> </td> </tr><tr><td><b>Live-cell compatible</b></td> <td align="center" style="text-align: center;">No</td> <td> </td> </tr><tr><td><b>Added to growth media</b></td> <td align="center" style="text-align: center;">No</td> <td> </td> </tr><tr><td><b>Formaldehyde fixable</b></td> <td align="center" style="text-align: center;">Labeled after fixation</td> <td> </td> </tr><tr><td><b>Multiplexing</b></td> <td align="center" style="text-align: center;"> </td> <td> </td> </tr><tr><td><b>Bibliography</b></td> <td align="center" style="text-align: center;"> </td> <td> </td> </tr><tr><td><b>Platform</b></td> <td align="center" style="text-align: center;"> </td> <td> </td> </tr><tr><td><b>Format</b></td> <td align="center" style="text-align: center;">10 plates/1,200 assays</td> <td> </td> </tr><tr><td><b>Cat. No.</b></td> <td align="center" style="text-align: center;"><a href="/order/catalog/product/H34158">H34158</a></td> <td> </td> </tr></tbody></table> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-79ec80a01b" class="cmp-container"> <div class="cmp-p-title"><div id="title-6bd819e116" class="cmp-title"> <h3 class="cmp-title__text">Phospholipidosis/Adipogenesis</h3> </div> </div> <div class="cmp-p-text"> <table class="rte-table"> <tbody><tr><td style="border: none;"> </td> <th><a href="/order/catalog/product/H34350">HCS LipidTOX Green Phospholipidosis Detection Reagent</a></th> <th><a href="/order/catalog/product/H34351">HCS LipidTOX Red Phospholipidosis Detection Reagent</a></th> </tr><tr><td><b>Readout</b></td> <td colspan="2" style="text-align: center;">Labels phospholipid accumulation after incubation with live cells</td> </tr><tr><td><b>Common filter set</b></td> <td style="background-color: rgb(0, 150, 97); color: rgb(255, 255, 255); text-align: center;">FITC</td> <td style="background-color: rgb(255, 57, 0); color: rgb(255, 255, 255); text-align: center;">Texas Red</td> </tr><tr><td><b>Reporter</b></td> <td style="background-color: rgb(0, 150, 97); color: rgb(255, 255, 255); text-align: center;">LipidTOX Green phospholipid stain</td> <td style="background-color: rgb(255, 57, 0); color: rgb(255, 255, 255); text-align: center;">LipidTOX Red phospholipid stain</td> </tr><tr><td><b>Ex/Em (nm)</b></td> <td style="background-color: rgb(0, 150, 97); color: rgb(255, 255, 255); text-align: center;" align="center">495/525</td> <td style="background-color: rgb(255, 57, 0); color: rgb(255, 255, 255); text-align: center;">595/615</td> </tr><tr><td><b>Live-cell compatible</b></td> <td colspan="2" style="text-align: center;">Yes</td> </tr><tr><td><b>Added to growth media</b></td> <td colspan="2" style="text-align: center;">Yes</td> </tr><tr><td><b>Formaldehyde fixable</b></td> <td colspan="2" style="text-align: center;">Yes</td> </tr><tr><td><b>Multiplexing</b></td> <td colspan="2" style="text-align: center;">Can be multiplexed with neutral lipid stains (see table above)</td> </tr><tr><td><b>Bibliography</b></td> <td colspan="2" style="text-align: center;"><a class="headline" href="/search/supportSearch?query=cytotoxicity%2C+imaging%2C+lipid&type=Citations+%26+References">Citations</a></td> </tr><tr><td><b>Platform</b></td> <td colspan="2" style="text-align: center;">Imaging, HCS</td> </tr><tr><td><b>Format</b></td> <td colspan="2" style="text-align: center;">125 μL (1,200 assays)</td> </tr><tr><td><b>Cat. No.</b></td> <td style="text-align: center;"><a href="/order/catalog/product/H34350">H34350</a></td> <td style="text-align: center;"><a href="/order/catalog/product/H34351">H34351</a></td> </tr></tbody></table> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-9ee1c2ee77"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-9ee1c2ee77-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-9ee1c2ee77-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS mitochondrial health assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-9ee1c2ee77-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-9ee1c2ee77-button"><div class="cmp-p-container"> <div id="container-9ee1c2ee77" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-acbe3d8cab" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1332828867" class="cmp-text"> <p>This automated assay uses Invitrogen MitoTracker Orange as an indicator of mitochondrial function because its accumulation in the mitochondria of live cells is proportional to the mitochondrial membrane potential. Invitrogen Hoechst 33342 is used as a segmentation tool to identify cells; a viability stain, such as the Invitrogen Image-iT DEAD Green Viability Stain, is easily incorporated to further multiplex the assay. These three dyes have sufficient retention of fluorescence signal intensity upon formaldehyde fixation and detergent permeabilization to be useful in fixed endpoint assays, as well as applications involving immunocytochemistry for specific protein detection.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Mitochondrial membrane potential</li> <li>Cell viability</li> <li>Nuclear shape and dimensions</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield</li> <li>10x or 20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/cell-viability.html" data-uw-rm-brl="false">Cell viability stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/apoptosis/mitochondria-function.html" data-uw-rm-brl="false">Mitochondria function assays</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-fdd2d0feea" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0 aem-GridColumn--default--4"> <div id="container-5e085d57dc" class="cmp-container"> <div class="aem-Grid aem-Grid--default--4 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--offset--phone--0 aem-GridColumn--default--4"><div class="cmp-textimage cta-container" id="textimage-78405207" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>HepG2 following <a href="/order/catalog/product/H3570">Hoechst 33342</a> staining to provide nuclear segmentation for automated cell counting and nuclear morphology measurement.</b></p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image1.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image1.jpg" data-title="HepG2 cells stained with Invitrogen Hoechst 33342 stain" id="textimage-78405207" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image1.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="HCS image showing fluorescent staining of nuclei and other cell structures" title="HepG2 cells stained with Invitrogen Hoechst 33342 stain"/> </noscript> <meta itemprop="caption" content="HepG2 cells stained with Invitrogen Hoechst 33342 stain"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0 aem-GridColumn--default--4"> <div id="container-d012807d60" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-1823646728" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b><a href="/order/catalog/product/I10291">Image-iT DEAD Green stain</a> used to label dead cells and provide a viability determination based on cell membrane permeability.</b></p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image2.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image2.jpg" data-title="Invitrogen Image-iT DEAD Green stain used to label dead cells " id="textimage-1823646728" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image2.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="HCS image showing fluorescent staining of dead cells" title="Invitrogen Image-iT DEAD Green stain used to label dead cells "/> </noscript> <meta itemprop="caption" content="Invitrogen Image-iT DEAD Green stain used to label dead cells "/> </div>  </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0 aem-GridColumn--default--4"> <div id="container-66769f73fb" class="cmp-container"> <div class="aem-Grid aem-Grid--default--4 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--offset--phone--0 aem-GridColumn--default--4"><div class="cmp-textimage cta-container" id="textimage-1237455376" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b><a href="/order/catalog/product/M7510">MitoTracker Orange</a> labels mitochondria with polarized membranes, with signal intensity proportional to membrane potential and mitochondrial health.</b></p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image3.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image3.jpg" data-title="MitoTracker Orange labels mitochondria with polarized membranes" id="textimage-1237455376" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondria-image3.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="HCS image of fluorescenct cells showing nuclear segmentation and mitochondrial membrane potential " title="MitoTracker Orange labels mitochondria with polarized membranes"/> </noscript> <meta itemprop="caption" content="MitoTracker Orange labels mitochondria with polarized membranes"/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-d1fcbda26b" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn aem-GridColumn--default--12"><div class="cmp-textimage cta-container" id="textimage-1555954778" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plot for HepG2 cells treated with increasing concentrations of valinomycin.</b> Cells were stained with Invitrogen <a href="/order/catalog/product/M7510">MitoTracker Orange</a> to measure mitochondrial membrane potential, Invitrogen <a href="/order/catalog/product/H3570">Hoechst 33342</a> dye to count total cells based on nuclear intensity, and Invitrogen <a href="/order/catalog/product/I10291">Image-iT DEAD Green</a> to identify dead cells. Automated imaging and analysis was done using Thermo Scientific HCS Platform and the data were used to calculate EC50 values for cell loss, mitochondrial membrane potential, and membrane permeability using GraphPad Prism software.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondrial-health-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondrial-health-dose-response.jpg" data-title="Dose-response plot for HepG2 cells treated with increasing concentrations of valinomycin" id="textimage-1555954778" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-mitochondrial-health-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph of three different mitochondrial assay parameters" title="Dose-response plot for HepG2 cells treated with increasing concentrations of valinomycin"/> </noscript> <meta itemprop="caption" content="Dose-response plot for HepG2 cells treated with increasing concentrations of valinomycin"/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-06eab52075"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-06eab52075-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-06eab52075-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS neurite outgrowth assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-06eab52075-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-06eab52075-button"><div class="cmp-p-container"> <div id="container-06eab52075" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-bc7e51eed2" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-277877796" class="cmp-text"> <p>This automated assay enables quantitation and correlation of neuron and neurite morphology. Analysis can range from simple neurite outgrowth measurement to complex analysis of extended neurite outgrowth and branching.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Cell count</li> <li>Cell size</li> <li>Neurite count</li> <li>Neurite length</li> <li>Neurite branching</li> <li>Since analysis is performed during image acquisition, it is possible to acquire an optimized data set to ensure the number of selected neurons to satisfy your statistical needs.</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield or confocal</li> <li>10x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/antibody/product/beta-3-Tubulin-Antibody-2G10-Monoclonal/MA1-118X" data-uw-rm-brl="false">Beta-3 tubulin antibody</a></li> <li><a href="/sg/en/home/life-science/antibodies/secondary-antibodies.html" data-uw-rm-brl="false">Secondary antibodies</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html" data-uw-rm-brl="false">Neuronal stains</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-1126855026" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-1185839998" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Primary neuron outgrowth assay.(Right)</b> A hippocampal primary neuron preparation was labeled using Invitrogen <a href="/order/catalog/product/H3570">Hoechst 33342</a> (blue) and an Alexa Fluor 647 immunoconjugate. <b>(Right)</b> Neurite outgrowth features were automatically identified using Thermo Scientific HCS Studio Software. Cell bodies are identified in blue, and neurons are identified in green.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-neurite-outgrowth-image.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-neurite-outgrowth-image.jpg" data-title="Primary neuron outgrowth assay " id="textimage-1185839998" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-neurite-outgrowth-image.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Two-panel HCS neurite outgrowth assay images showing stained cells and software overlay" title="Primary neuron outgrowth assay "/> </noscript> <meta itemprop="caption" content="Primary neuron outgrowth assay "/> </div>  </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-40bdc1bcf9" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-1311676770" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plots showing the effect of glutamate and kainite concentration on rat hippocampal neurons using automated measurements of neuron number, neurite count, neurite length, and neurite intensity.</b></p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-neurite-outgrowth-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-neurite-outgrowth-dose-response.jpg" data-title="Dose-response plots " id="textimage-1311676770" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-neurite-outgrowth-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Four-panel graph of different neurite outgrowth assay parameters" title="Dose-response plots "/> </noscript> <meta itemprop="caption" content="Dose-response plots "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-d56101e09e"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-d56101e09e-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-d56101e09e-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS oxidative stress assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-d56101e09e-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-d56101e09e-button"><div class="cmp-p-container"> <div id="container-d56101e09e" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-628ac8d93e" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-413934304" class="cmp-text"> <p>Using fluorogenic reporters such as CellROX dyes allows for the real-time analysis of ROS generation in living cells and in fixed-cell preparations.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Fluorescence intensity, morphology, and count values for each object</li> <li>Fluorescence intensity, morphology, and count values of spots in different cell regions</li> <li>Average fluorescence intensity difference and ratios between different regions for each cell</li> <li>Intensity and count ratio between channels within different regions for each cell</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms- second image was imaged using the CX5 platform</li> <li>Widefield or confocal</li> <li>10x or 20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/oxidative-stress.html" data-uw-rm-brl="false">CellROX Reagents</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-6ca51bca60" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-1765527138" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><strong>Hap1 cells were treated with menadione and labeled with <a href="/order/catalog/product/C10444">CellROX Green</a> and <a href="/order/catalog/product/H1399">Hoechst 33342</a>. </strong>The cells were imaged using a <a href="/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/platforms/cx5.html">Thermo Scientific CellInsight CX5 high-content platform</a> (row A), and <a href="/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html">Thermo Scientific HCS Studio Software</a>, which were used to automatically identify (row B) and quantify the intensity of CellROX green fluorescence from each cell.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-oxidative-stress.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-oxidative-stress.jpg" id="textimage-1765527138" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-oxidative-stress.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt/> </noscript> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-095cb0772b" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-125002791" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-dependent increase in ROS production in Hap 1 cells loaded with <a href="/order/catalog/product/C10444">CellROX Green</a> and treated with menadione for one hour.</b></p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-oxidative-stress-data.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-oxidative-stress-data.jpg" data-title="Dose-dependent increase in ROS production in Hap 1 cells " id="textimage-125002791" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-oxidative-stress-data.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="HCS Oxidative Stress Assay sample data" title="Dose-dependent increase in ROS production in Hap 1 cells "/> </noscript> <meta itemprop="caption" content="Dose-dependent increase in ROS production in Hap 1 cells "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-2eda4714fa"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-2eda4714fa-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-2eda4714fa-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS protein synthesis assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-2eda4714fa-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-2eda4714fa-button"><div class="cmp-p-container"> <div id="container-2eda4714fa" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-3064b63317" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1123033961" class="cmp-text"> <p>The Click-iT Plus OPP Alexa Fluor Protein Synthesis Assay Kit provides a fast, sensitive method for the detection of protein synthesis in a HCS format. The assay incorporates O-propargyl-puromycin (OPP) efficiently into newly translated proteins in a complete methionine-containing medium. The protein is then fluorescently labeled with a bright, photostable Alexa Fluor dye in a fast, highly specific, mild click reaction.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Fluorescence intensity, morphology, and count values for each object</li> <li>Fluorescence intensity, morphology, and count values of spots in different cell regions</li> <li>Average fluorescence intensity difference and ratios between different regions for each cell</li> <li>Intensity and count ratio between channels within different regions for each cell</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms- second image was imaged using the CX5 platform</li> <li>Widefield or confocal</li> <li>10x or 20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/protein-synthesis-assays.html" data-uw-rm-brl="false">Protein synthesis assay kits</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-05ac3e27ad" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"> <div id="container-17fecc34f0" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis.jpg" id="image-1110975329" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-apoptosis.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt/> </noscript> </div>  </div> <div class="cmp-p-container"> <div id="container-acf15490c5" class="cmp-container"> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-2113727161" class="cmp-text"> <p><strong>Hap1 cells treated with cyclohexamide and labeled with <a href="/order/catalog/product/H1399">Hoechst 33342</a> and Click-iT OPP followed by addition of an Alexa Fluor 488 azide for chemoselective detection (<a href="/order/catalog/product/C10456">Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit</a>). </strong>The cells were imaged using a <a href="/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/platforms/cx5.html">Thermo Scientific CellInsight CX5 high-content platform CellInsight CX5 high-content platform</a> (row A) and <a href="/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html">Thermo Scientific HCS Studio Software</a>, which were used to automatically identify (row B) and quantify the intensity of Click-iT OPP green fluorescence from each cell.</p> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-198296ebe8" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-278406651" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-dependent decrease in protein synthesis following treatment of cells with cyclohexamide.</b> The sensitivity of two cell types: wild type Hap1 (red) and Hap1 lacking ATG5 (blue), were compared.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-protein-systhesis-data.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-protein-systhesis-data.jpg" data-title="Dose-dependent decrease in protein synthesis " id="textimage-278406651" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-protein-systhesis-data.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="HCS Protein Synthesis sample data" title="Dose-dependent decrease in protein synthesis "/> </noscript> <meta itemprop="caption" content="Dose-dependent decrease in protein synthesis "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-098d7b2a5f"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-098d7b2a5f-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-098d7b2a5f-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS stem cell differentiation assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-098d7b2a5f-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-098d7b2a5f-button"><div class="cmp-p-container"> <div id="container-098d7b2a5f" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-10626ece70" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-247678660" class="cmp-text"> <p>This automated assay monitors the transition of pluripotent stem cells (PSC) to a mesodermal and then committed cardiomyocyte phenotype using a fixed-cell imaging protocol. In a 3-channel assay, cells are fixed and stained with a nuclear stain for localization and with primary antibodies against nuclear transcription factors associated with pluripotency (Oct4) and cardiac differentiation (Nkx2.5).</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Nuclear mask identification</li> <li>Spots identified and localized by channel</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield</li> <li>10x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/sg/en/home/life-science/antibodies/secondary-antibodies.html" data-uw-rm-brl="false">Secondary antibodies</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-3d3c32e4fe" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-814515917" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Immunofluorescent detection of Oct4 (green) and NKx2.5 (red) to determine differentiation status in H9 cells stained with DAPI (blue).</b></p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-image1.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-image1.jpg" data-title="Immunofluorescent detection of Oct4 and NKx2.5 " id="textimage-814515917" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-image1.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Three-panel HCS image showing stem cell marker appearance over the assay course" title="Immunofluorescent detection of Oct4 and NKx2.5 "/> </noscript> <meta itemprop="caption" content="Immunofluorescent detection of Oct4 and NKx2.5 "/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-31f9db90e6" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-727620494" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Identifying differentiated stem cells. </b>DAPI staining is used to automatically generate a nuclear mask (blue outline), then cells positive for the differentiation marker (in this case Oct4) within the nuclear region are highlighted in red.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-image2.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-image2.jpg" data-title="Identifying differentiated stem cells" id="textimage-727620494" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-image2.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Two-color HCS image showing nuclei and Oct4 location" title="Identifying differentiated stem cells"/> </noscript> <meta itemprop="caption" content="Identifying differentiated stem cells"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-aaaa36bb78" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-1611083554" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Results from stem cell differentiation assay. </b>The percentages of Oct4+ and Nkx2.5+ nuclei were quantified in cells fixed at the indicated time points. Prior to differentiation (Day 0), nearly 100% of all cells were Oct4+/Nkx2.5–, consistent with a pluripotent state. At Day 6, greater than 90% of cells analyzed were co-positive for both markers. After day 6, the frequency of Oct4+ cells declined, consistent with a loss of pluripotency and a transition to a terminally differentiated cardiomyocyte phenotype.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-quantification.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-quantification.jpg" data-title="Results from stem cell differentiation assay" id="textimage-1611083554" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-stem-cell-differentiation-quantification.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Bar chart showing two stem cell marker measurements over time" title="Results from stem cell differentiation assay"/> </noscript> <meta itemprop="caption" content="Results from stem cell differentiation assay"/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-c183f67eb1"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-c183f67eb1-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-c183f67eb1-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS synaptogenesis assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-c183f67eb1-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-c183f67eb1-button"><div class="cmp-p-container"> <div id="container-c183f67eb1" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-2ed8e6cb06" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1688756580" class="cmp-text"> <p>This automated assay uses the co-localization of presynaptic and postsynaptic markers to identify synapses and correlate them with neuronal and neurite morphology.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Neurite count</li> <li>Neurite length</li> <li>Neurite branching</li> <li>Presynaptic vesicles</li> <li>Postsynaptic spots</li> <li>Synapse number</li> <li>Since analysis is performed during image acquisition, it is possible to acquire an optimized data set to ensure the number of selected neurons to satisfy your statistical needs.</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield or confocal</li> <li>20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/antibody/product/beta-3-Tubulin-Antibody-2G10-Monoclonal/MA1-118X" data-uw-rm-brl="false">Beta-3 tubulin antibody</a></li> <li><a href="/antibody/product/Synaptophysin-Antibody-Polyclonal/PA1-1043" data-uw-rm-brl="false">Synaptophysin antibody</a></li> <li><a href="/sg/en/home/life-science/antibodies/secondary-antibodies.html" data-uw-rm-brl="false">Secondary antibodies</a></li> <li><a href="/antibody/product/PSD95-Antibody-7E3-1B8-Monoclonal/MA1-046" data-uw-rm-brl="false">PSD95 antibody</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-0495a38278" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-333362220" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><strong>Synaptogenesis assay.(Left)</strong> A hippocampal primary neuron preparation was labeled using Invitrogen <a href="/order/catalog/product/H3570">Hoechst 33342 stain</a> (blue) and an Invitrogen Alexa Fluor 488 MAP2 antibody conjugate. <strong>(Center and Right)</strong> Synaptogenesis features were automatically identified using Thermo Scientific HCS Studio Software. Cell bodies are identified in blue, and spots are identified in pink. Neurites are identified in green, and overlaps between spots and neurites are labeled in yellow.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-synaptogenesis-image.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-synaptogenesis-image.jpg" data-title="Synaptogenesis assay and an Alexa Fluor 488 MAP2 antibody conjugate " id="textimage-333362220" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-synaptogenesis-image.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Three-panel HCS synaptogenesis assay image showing stained cells and software overlays " title="Synaptogenesis assay and an Alexa Fluor 488 MAP2 antibody conjugate "/> </noscript> <meta itemprop="caption" content="Synaptogenesis assay and an Alexa Fluor 488 MAP2 antibody conjugate "/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-0d033d10a9" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-469974590" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plots showing the effect of glutamate and kainite concentration on rat hippocampal neurons using automated measurements of presynaptic intensity, postsynaptic intensity, vesicle counts, and synapse counts.</b></p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-synaptogenesis-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-synaptogenesis-dose-response.jpg" data-title="Effect of glutamate and kainite concentration on rat hippocampal neurons " id="textimage-469974590" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-synaptogenesis-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Four-panel graph of different synaptogenesis assay parameters " title="Effect of glutamate and kainite concentration on rat hippocampal neurons "/> </noscript> <meta itemprop="caption" content="Effect of glutamate and kainite concentration on rat hippocampal neurons "/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-2958334095"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-2958334095-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-2958334095-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS transcription factor activation</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-2958334095-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-2958334095-button"><div class="cmp-p-container"> <div id="container-2958334095" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-0486b4f0cc" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1028248243" class="cmp-text"> <p>In this automated assay, translocation of the activated transcription factor from the cytoplasm to the nucleus is automatically detected and quantified for each cell. The assay is conveniently run using three detection channels, with a nuclear stain, a whole-cell stain, and a specific label for the transcription factor.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Transcription factor intensity in the cytoplasm and in the nucleus</li> <li>Difference in transcription factor intensity between the cytoplasm and nucleus, as a measure of cytoplasm-to-nucleus translocation</li> <li>Ratio of transcription factor intensity between the cytoplasm and nucleus, as a measure of cytoplasm-to-nucleus translocation</li> <li>Percentage of cells in the well above a user-definable threshold for the nucleus-to-cytoplasm intensity difference or ratio for one or more targets</li> <li>Nuclear shape and dimensions</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield</li> <li>10x or 20x magnification</li> </ul> <p><b>Protocols and reagents:</b></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> <li><a href="/antibody/product/NFkappaB-p65-Antibody-Polyclonal/PA5-16545" data-uw-rm-brl="false">NFκB detection</a></li> <li><a href="/sg/en/home/life-science/antibodies/secondary-antibodies.html" data-uw-rm-brl="false">Secondary antibodies</a></li> <li>Additional transcription factors of signaling molecules detected in additional channels</li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-d264b725bd" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-927353216" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><strong>Transcription factor activation(Left)</strong> HeLa cells were treated with TNFα, fixed, permeabilized, and stained with Invitrogen <a href="/order/catalog/product/H10325">HCS NuclearMask Blue</a> (blue), and the NFκB was labeled by indirect immunofluorescence (green). <strong>(Right)</strong> Cell features for the assay were automatically detected using Thermo Scientific HCS Studio Software: nucleus (blue overlay), cytoplasm (green overlay), and NFκB intensity. The red overlay shows the cells where translocation did not occur.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-image.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-image.jpg" data-title="Transcription factor activation of HeLa cells " id="textimage-927353216" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-image.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Two-panel HCS NFκB translocation assay image showing stained cells and software overlay" title="Transcription factor activation of HeLa cells "/> </noscript> <meta itemprop="caption" content="Transcription factor activation of HeLa cells "/> </div>  </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-b8218d593b" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-279131255" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>NFκB cytoplasm-nucleus intensity difference vs. ratio.</b> NFκB intensity differences between the cytoplasm and nucleus were automatically calculated and plotted. Red dots in the scatter plot of the NFκB cytoplasm-nucleus intensity difference versus ratio correspond to cells that did not exhibit NFκB translocation.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-image3.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-image3.jpg" data-title="NFκB cytoplasm-nucleus intensity difference vs. rati" id="textimage-279131255" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-image3.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph of measured NFκB translocation parameters " title="NFκB cytoplasm-nucleus intensity difference vs. rati"/> </noscript> <meta itemprop="caption" content="NFκB cytoplasm-nucleus intensity difference vs. rati"/> </div>  </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-b156bc488e" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-2049834801" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Dose-response plot showing the activation of NFkB by increasing TNFα concentration.</b> The y-axis shows the intensity difference between the nucleus (containing translocated NFkB) and the surrounding cytoplasm (containing un-translocated NFkB) as a measure of NFkB translocation.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-activation-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-activation-dose-response.jpg" data-title="Activation of NFkB by increasing TNFα concentration" id="textimage-2049834801" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-transcription-factor-activation-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph showing relationship between NFκB activation and TNFα concentration" title="Activation of NFkB by increasing TNFα concentration"/> </noscript> <meta itemprop="caption" content="Activation of NFkB by increasing TNFα concentration"/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-7076821520"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-7076821520-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-7076821520-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS viability assay</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-7076821520-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-7076821520-button"><div class="cmp-p-container"> <div id="container-7076821520" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-1ce420b62e" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1989628483" class="cmp-text"> <p>Membrane integrity is a commonly measured parameter in cell viability assays. Cells with compromised membranes allow entry to otherwise cell-impermeant DNA-binding dyes, and this DNA staining serves as a cell death indicator that can be used as the basis for a single-channel assay. Invitrogen Image-iT DEAD Green Viability Stain (green; <a href="/order/catalog/product/I10291" data-uw-rm-brl="false">Cat. No. I10291</a>) is an ideal dead cell stain because it will label the nuclei of dead cells and also survives fixation and permeabilization so that it can be readily multiplexed with other techniques.</p> <p> </p> <p><strong>Automatically measured properties: </strong></p> <ul> <li>Single-channel</li> <li>Two-channel</li> <li>Total cells</li> <li>Live cells</li> <li>Dead cells</li> </ul> <p><strong>Imaging mode: </strong></p> <ul> <li>CellInsight CX5 and CX7 Platforms</li> <li>Widefield</li> <li>10x magnification</li> </ul> <p><strong>Protocols and reagents:</strong></p> <ul> <li><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/cell-viability.html" data-uw-rm-brl="false">Viability stains</a></li> <li><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html" data-uw-rm-brl="false">Nuclear stains</a></li> </ul> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-edd9b56754" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-5375969" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Single-channel viability assay with multiplexing. </b>HeLa cells treated with camptothecin and stained with Invitrogen <a href="/order/catalog/product/H3570">Hoechst 33342</a> (blue channel) for a total cell count. Dead cells are stained with Invitrogen <a href="/order/catalog/product/I10291">Image-iT DEAD Green</a> (green channel) and proliferating cells are labeled using Invitrogen <a href="/order/catalog/product/C10640">Click-iT PLUS EdU with Invitrogen Alexa Fluor 647 picolyl azide</a> (deep red channel).</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-single-channel.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-single-channel.jpg" data-title="Single-channel viability assay with multiplexing HeLa cells" id="textimage-5375969" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-single-channel.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Eight-panel HCS image showing 3 viability parameters at 2 camptothecin concentrations" title="Single-channel viability assay with multiplexing HeLa cells"/> </noscript> <meta itemprop="caption" content="Single-channel viability assay with multiplexing HeLa cells"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-7a71cbf9e8" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-1612440874" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Two-channel viability assay. </b>A549 cells were treated with varying concentrations of camptothecin and labeled with the Invitrogen <a href="/order/catalog/product/R37601">LIVE/DEAD Cell Imaging Kit</a>. Living cells sequester the LIVE Green probe, whereas dead cells become permeable to the DEAD Red stain. Cells were counterstained with Invitrogen <a href="/order/catalog/product/H3570">Hoechst 33342</a> for total cell count.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-2channel.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-2channel.jpg" data-title="Two-channel viability assay of A549 cells" id="textimage-1612440874" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-2channel.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Microscopic view of A549 cells stained in 3-color fluorescence, indicating live vs dead cells" title="Two-channel viability assay of A549 cells"/> </noscript> <meta itemprop="caption" content="Two-channel viability assay of A549 cells"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-8ad59793d2" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-1249392695" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Single-channel viability assay with multiplexing. </b>Dose-response curve for HeLa cells treated with camptothecin, stained with Invitrogen <a href="/order/catalog/product/I10291">Image-iT DEAD Green</a> and measured using the Thermo Scientific CellInsight CX5 High Content Screening Platform.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-single-channel-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-single-channel-dose-response.jpg" data-title="Single-channel viability assay with multiplexing" id="textimage-1249392695" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-single-channel-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph of dead cells vs camptothecin " title="Single-channel viability assay with multiplexing"/> </noscript> <meta itemprop="caption" content="Single-channel viability assay with multiplexing"/> </div>  </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-dc9c6ab079" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-textimage m--teaser aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"><div class="cmp-textimage cta-container" id="textimage-633985839" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Two-channel viability assay. </b>Dose-response plot showing the effect of camptothecin concentration on cell viability using the Invitrogen <a href="/order/catalog/product/R37601">LIVE/DEAD Cell Imaging Kit</a>.</p> </div> <div class="cmp-ctalist"> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-two-channel-dose-response.jpg" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-two-channel-dose-response.jpg" data-title="Two-channel viability assay" id="textimage-633985839" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/hcs-viability-two-channel-dose-response.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph of two viability parameters" title="Two-channel viability assay"/> </noscript> <meta itemprop="caption" content="Two-channel viability assay"/> </div>  </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-c0d13fbb1c-item-4ae59f6bfb"> <h2 class="cmp-accordion__header"> <button id="accordion-c0d13fbb1c-item-4ae59f6bfb-button" class="cmp-accordion__button" aria-controls="accordion-c0d13fbb1c-item-4ae59f6bfb-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">HCS image segmentation assays</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-c0d13fbb1c-item-4ae59f6bfb-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-c0d13fbb1c-item-4ae59f6bfb-button"><div class="cmp-p-container"> <div id="container-4ae59f6bfb" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-f51b07cfd2" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-dc71413a73" class="cmp-container"> <div class="cmp-p-text alt"> <div id="text-682225725" class="cmp-text"> <p>Image segmentation separates objects of interest (cells) from background or other features not relevant to the analysis and allows determination of items of interest in their appropriate spatial context such as translocation of biomarkers into the nucleus or changes in biomarker levels within particular cellular compartments. Key approaches for image segmentation involve using fluorescent stains selective for specific parts of the cell—nucleus, cytoplasm, plasma membrane, or other organelles—to delineate the whole cell.</p> <p> </p> <p><b>Automatically measured properties: </b></p> <ul> <li>Nuclear</li> <li>Cytoplasmic</li> <li>Plasma membrane</li> <li>Organelle</li> </ul> <p><b>Imaging mode: </b></p> <ul> <li>CellInsight CX5 and CX7 Platforms </li> <li>Widefield<br /> </li> <li>10x magnification</li> </ul> <p><b>Reagents</b></p> <ul> <li>Learn more about <a href="/sg/en/home/life-science/cell-analysis/cell-structure.html" data-uw-rm-brl="false">products for Cell Structure</a><br /> </li> </ul> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-d94d9e64a8" class="cmp-container"> <div class="aem-Grid aem-Grid--default--10 aem-Grid--phone--12 "> <div class="cmp-p-container aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0"> <div id="container-d17db90793" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/560-wide.par.44769.image.535.221.1.s007070-cytochalasin-d-gif.gif" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/560-wide.par.44769.image.535.221.1.s007070-cytochalasin-d-gif.gif" id="image-1987151756" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/560-wide.par.44769.image.535.221.1.s007070-cytochalasin-d-gif.gif" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt/> </noscript> </div>  </div> <div class="cmp-p-container"> <div id="container-8169025171" class="cmp-container"> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-1865537722" class="cmp-text"> <p><b>Figure 2. The effects of cytochalasin D treatment on HeLa cell size as measured by <a href="/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=H32720">HCS CellMask Blue stain</a>.</b> HeLa cells were treated with DMSO vehicle (left) or 10 µM cytochalasin D (right) for 3 hr before fixation and permeabilization. Samples were then labeled with <a href="/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=H32720">HCS CellMask Blue stain</a>, <a href="/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=A12379">Alexa Fluor 488 dye–conjugated phalloidin</a> to visualize filamentous actin (red), mouse anti–α-tubulin IgG (detected with <a href="/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=A21422">Alexa Fluor 555 goat anti-mouse IgG</a>, green), and <a href="/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=T3605">TO-PRO-3 iodide</a> to counterstain nuclei (magenta). The bar graph represents quantitative measurements of cell size as determined by HCS CellMask Blue stain to show the effects of cytochalasin D treatment.</p> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--10 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-438a90888f" class="cmp-container"> <div class="cmp-p-textimage m--teaser"><div class="cmp-textimage cta-container" id="textimage-1012693394" data-cmp-hook-cta="ctapanel"> <div class="cmp-textoverlay"> <div class="cmp-textoverlay__textwrapper"> <h2 class="cmp-textoverlay__title"></h2> <p><b>Figure 3. Plasma membrane image segmentation of live cells using <a href="/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=C10046">CellMask Deep Red plasma membrane stain</a>.</b> HaCaT cells (an immortalized human keratinocyte cell line) transfected with a mitochondrion-targeted eGFP (pseudocolored green) construct were incubated with 1 µM <a href="/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=H3570">Hoechst 33342</a> (pseudocolored blue) and then 8 μg⁄mL <a href="/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=C10046">CellMask Deep Red plasma membrane stain</a> (pseudocolored red). Cells were imaged with a Zeiss Cell Observer HS microscope. The image stack was deconvolved with the Zeiss AxioVision 3D Deconvolution module with the point spread function acquired using a 200 nm TetraSpeck fluorescent microsphere. Image submitted by Christian Junker, University of Saarland, Department of Biophysics, Homburg, Germany.</p> </div> </div> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/sample-data/275-wide.par.33322.image.275.279.1.s005081-hcs-gif.gif" data-asset="/content/dam/tfsite/images/storefront/hcs/sample-data/275-wide.par.33322.image.275.279.1.s005081-hcs-gif.gif" id="textimage-1012693394" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/sample-data/275-wide.par.33322.image.275.279.1.s005081-hcs-gif.gif" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt/> </noscript> </div>  </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-b5445e2fb6" class="cmp-container"> <div class="cmp-p-productlist"> <div id="skulist" style="display:none">H10325,H10326,H10294,H3570,H32720,H32714,H32713,H32712,H32721,H32722,C10045,C10046</div> <div id='ltajax-7228466935587024781'></div><script type="text/javascript">(function() {$('#ltajax-7228466935587024781').load('/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/sample-data/jcr:content/root/container_442015454/container_1002513207/sectioncontainer_192_1346680907/container/accordion/item_1622044998236/container_774466532/container/productlist.ajax.html?skulist=');})(); </script> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer mt-l1 p-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-53d922e5c3"> <div class="cmp-sectiontitle"> <h2 id="instrument-antibodies-assays" class="cmp-sectiontitle__text" data-anchor-nav-title="High-content antibodies and assays">High-content antibodies and assays</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container mt-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-e8bdd647f1" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-text alt aem-GridColumn aem-GridColumn--default--12"> <div id="text-1110536279" class="cmp-text"> <p>Learn more about <a>reagents for </a><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation.html">cell viability, proliferation, and function</a> or <a></a><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if.html">reagents to determine cell structure</a>.</p> </div> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-a02400b47c" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-2064420384" class="cmp-text"> <p>For a more specific primary or secondary antibody, make your selection using these links:</p> </div> </div> <div class="cmp-p-ctaitem"><div class="cmp-ctaitem" id="ctaitem-ac82ebed88"> <a class="cmp-ctaitem__anchor" href="/antibody/primary/query/"> <span class="cmp-ctaitem__icon icon-24-arrow-right-mono"></span> <span class="cmp-ctaitem__text">Primary antibodies</span> </a> </div> </div> <div class="cmp-p-ctaitem"><div class="cmp-ctaitem" id="ctaitem-a0939e33b4"> <a class="cmp-ctaitem__anchor" href="/antibody/secondary/query/"> <span class="cmp-ctaitem__icon icon-24-arrow-right-mono"></span> <span class="cmp-ctaitem__text">Secondary antibodies</span> </a> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-4fe3653e4a" class="cmp-container"> <div class="cmp-p-separator"> <div id="separator-730a3847a6" class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer p-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-c00f4a2e5d"> <div class="cmp-sectiontitle"> <h2 id="image-gallery" class="cmp-sectiontitle__text" data-anchor-nav-title="Image gallery">Image gallery</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-90de430351" class="cmp-container"> <div class="cmp-p-title"><div id="title-688fd1c271" class="cmp-title"> <h3 class="cmp-title__text">Images obtained from the CellInsight CX7 LZR platform</h3> </div> </div> </div> </div> <div class="cmp-p-container p-l1 m--learning-center-container-border mt-l1 mb-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-a04731c925" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/07/hcs-hca-compose-fireplex-1200x1200.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/07/hcs-hca-compose-fireplex-1200x1200.jpg" data-title="High-throughput immunoassay screening" id="image-2076688394" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/07/hcs-hca-compose-fireplex-1200x1200.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="High-throughput immunoassay screening" title="High-throughput immunoassay screening"/> </noscript> <meta itemprop="caption" content="High-throughput immunoassay screening"/> </div>  </div> <div class="cmp-p-text mt-l1"> <div id="text-1457351440" class="cmp-text"> <p><b>High-throughput immunoassay screening enabled using the new CellInsight CX7 Pro Platforms</b></p> <p> </p> <p>Comparison of the FirePlex™-HT no-wash immunoassay between the CellInsight CX7 and CX7 Pro instruments. The no-wash FirePlex assay optimized for high-throughput screening was performed according to the manufacturer’s protocol for detecting both 5 and 10 analytes per well. A 10-point standard curve was quantified to assay analyte reproducibility and dynamic range windows. The resulting assay was measured using either the CellInsight CX7 (left images) or CX7 Pro (right images) platforms and quantified using the FirePlex Analysis Workbench software. Only the CX7 Pro instrument (right images) was able to exceed 2.5 (15-bit) or 3.0 (16-bit) dynamic range and less than 15 percent intra-plate CVs. The significantly improved dynamic range and reproducibility performance is due to the CX7 Pro instrument's superior optics and 95% QE detection capability. This experiment was validated by Abcam™ using both 15- and 16-bit CX7 Pro sCMOS camera modes for HT-screening considerations.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 m--learning-center-container-border mt-l1 mb-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-0476555e8a" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/3.png" data-asset="/content/dam/tfsite/images/storefront/hcs/3.png" data-title="Immuno-oncology application: Imaging antibody-dependent cell killing in breast cancer spheroids" id="image-232753426" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/3.png" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Immuno-oncology application: Imaging antibody-dependent cell killing in breast cancer spheroids" title="Immuno-oncology application: Imaging antibody-dependent cell killing in breast cancer spheroids"/> </noscript> <meta itemprop="caption" content="Immuno-oncology application: Imaging antibody-dependent cell killing in breast cancer spheroids"/> </div>  </div> <div class="cmp-p-text mt-l1"> <div id="text-269907224" class="cmp-text"> <p><b>Immuno-oncology application: Imaging antibody-dependent cell killing in breast cancer spheroids</b></p> <p> </p> <p><b>Antibody-dependent cell killing in breast cancer spheroids. </b>Human natural killer cells isolated with <a href="/order/catalog/product/11349D#/11349D" data-uw-rm-brl="false">Dynabeads Untouched Human NK Cells Kit</a> were labeled with <a href="/order/catalog/product/C34565#/C34565" data-uw-rm-brl="false">CellTracker Deep Red dye</a>. Human breast cancer cells (SKBR3) were grown overnight in Nunclon Sphera 96-well plates to form spheroids, treated with the anti-HER2 antibody Trastuzumab, then challenged with NK cells for 4 hours. Cells were stained with <a href="/order/catalog/product/C10723#/C10723" data-uw-rm-brl="false">CellEvent Caspase-3/7 Green Detection Reagent</a> and Hoechst 33342 and imaged on the CellInsight CX7 LZR High-Content Screening. Platform. The images are maximum intensity projection of multiple Z sections. CellEvent Caspase-3/7 Green Detection Reagent was used to study NK cell and antibody-mediated apoptosis in spheroids. CellTracker Deep Red was used to track NK cells within the spheroid. Trastuzumab bound to HER2 on SKBR3 cells amplifies the NK cell anti-tumor response via antibody-dependent cellular apoptosis.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 m--learning-center-container-border mt-l1 mb-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-54c3f86713" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/9.png" data-asset="/content/dam/tfsite/images/storefront/hcs/9.png" data-title="Spheroid imaging and analysis application: segmentation and quantitation of EdU proliferation and spheroid size" id="image-1615310713" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/9.png" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Spheroid imaging and analysis application: segmentation and quantitation of EdU proliferation and spheroid size" title="Spheroid imaging and analysis application: segmentation and quantitation of EdU proliferation and spheroid size"/> </noscript> <meta itemprop="caption" content="Spheroid imaging and analysis application: segmentation and quantitation of EdU proliferation and spheroid size"/> </div>  </div> <div class="cmp-p-text mt-l1"> <div id="text-1472234591" class="cmp-text"> <p><b>Spheroid imaging and analysis application: segmentation and quantitation of EdU proliferation and spheroid size</b></p> <p> </p> <p><b>Segmentation and quantitation of EdU and spheroid size using CellInsight CX7 LZR system.</b> A549 cells were plated at a density of 5,000 cells per well on a Nunclon Sphera 96U-well microplate and incubated for 24 hours in a CO2 incubator. EdU was added at a final concentration of 10 µM and incubated for 1 hr. The spheroids were then washed and fixed with 4% formaldehyde and permeabilized with 0.25% Triton X-100. The spheroids were then stained for EdU using the <a href="/order/catalog/product/C10351#/C10351" data-uw-rm-brl="false">Click-iT EdU Alexa Fluor 488 HCS Assay Kit</a> following the kit protocol. The plate was imaged with a 4x objective using confocal mode on a CellInsight CX7 LZR High Content Screening Platform. The image is a maximum intensity projection of 200 optical z-slices of 1 micron each. Quantitation was performed with HCS Studio 2.0 software using the Morphology Explorer bio-application. The spheroid was segmented as one object, and EdU positive cells were counted as spots within the spheroid. Using Morphology Explorer bio-application, the spheroids were segmented as a whole object, and EdU positive cells were segmented inside the spheroid and number of cells was plotted.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 m--learning-center-container-border mt-l1 mb-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-f8c7df94e3" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/2020/live-cell-confocal-imaging-3d-spheroids-using-hcs-system-1000x738.png" data-asset="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/2020/live-cell-confocal-imaging-3d-spheroids-using-hcs-system-1000x738.png" data-title="Confocal imaging of 3D spheroids in live cell mode for bio-applications" id="image-1778677283" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/2020/live-cell-confocal-imaging-3d-spheroids-using-hcs-system-1000x738.png" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Confocal imaging of 3D spheroids in live cell mode for bio-applications" title="Confocal imaging of 3D spheroids in live cell mode for bio-applications"/> </noscript> <meta itemprop="caption" content="Confocal imaging of 3D spheroids in live cell mode for bio-applications"/> </div>  </div> <div class="cmp-p-text mt-l1"> <div id="text-774228041" class="cmp-text"> <p><b>Confocal imaging of 3D spheroids in live cell mode for bio-applications</b></p> <p> </p> <p><b>Confocal imaging of 3D spheroids in live cell mode.</b> <b>(A)</b> A549 cells were plated at a density of 5,000/well on a U-bottom plate and incubated for 48 hr in a CO2 incubator. Live spheroids were labelled for live and dead cells with a <a href="/order/catalog/product/L3224#/L3224" data-uw-rm-brl="false">LIVE/DEAD Viability/Cytotoxicity Kit</a>. The plate was automatically imaged with 10x objective using confocal on a CellInsight CX7 LZR HCS instrument. The image is a maximum intensity projection of multiple z-sections. Dead cells stained were observed in spheroid core (red) and live cells (green) were observed on the periphery of spheroids. <b>(B)</b> A549 cells were plated at a density of 5,000/well on a U-bottom plate and incubated for 24 hr in the CO2 incubator. Live spheroids were then stained with <a href="/order/catalog/product/M7510#/M7510" data-uw-rm-brl="false">MitoTracker Orange CMTMRos</a> and <a href="/order/catalog/product/C10723#/C10723" data-uw-rm-brl="false">CellEvent Caspase-3/7 Green Detection Reagent</a> for 30 min. The plate was automatically imaged with 10x objective using confocal on a CellInsight CX7 LZR HCS instrument. The image is a maximum intensity projection of multiple Z sections. In A549 live spheroids, most of the cells are healthy as seen by MitoTracker Orange staining, and very few apoptotic cells were observed. <b>(C)</b> A549 cells were plated at a density of 5,000/well on a U-bottom plate and incubated for 24 hr in a CO2 incubator. Live spheroids were then stained with <a href="/order/catalog/product/I14834#/I14834" data-uw-rm-brl="false">Image-iT Green Hypoxia Reagent</a> for 30 min. The plate was automatically imaged with 10x objective using confocal on a CellInsight CX7 LZR HCS instrument. The image is a maximum intensity projection of multiple z-sections. Live A549 spheroids show hypoxia staining as stained with Image-iT Green Hypoxia Reagent. <b>(D)</b> SKBR3 cells were plated at a density of 5,000/well on a U-bottom plate and incubated for 24 hr in a CO2 incubator. Live spheroids were then incubated with pHrodo Red conjugated Herceptin antibody for 24 hr. The plate was automatically imaged with 4x objective using confocal on a CellInsight CX7 LZR HCS instrument. The image is a maximum intensity projection of multiple z-sections. The internalized pHrodo conjugated Herceptin antibody is observed in the intracellular vesicles. <b>(E)</b> Neurospheres were differentiated from neural stem cells (NSC) in <a href="/order/catalog/product/A3582901#/A3582901" data-uw-rm-brl="false">Neurobasal Plus Medium</a> with <a href="/order/catalog/product/A3320201#/A3320201" data-uw-rm-brl="false">Culture One Supplement</a>. Live neurospheres were then stained with <a href="/order/catalog/product/T34077#/T34077" data-uw-rm-brl="false">Tubulin Tracker Deep Red</a> for 1 hr. The plate was automatically imaged with 4X objective using confocal on a CellInsight CX7 LZR HCS instrument. The image is a maximum intensity projection of multiple z-sections. Tubulin Tracker Deep Red stains the neurites in the neurospheres differentiated from NSC. <b>(F)</b> HeLa cells were plated at a density of 5,000/well on a U-bottom plate and incubated for 24 hr in a CO2 incubator. Activated T cells were labelled with <a href="/order/catalog/product/C34565#/C34565" data-uw-rm-brl="false">CellTracker Deep Red</a> and about 5,000 cells were added to each well. After 2 hr incubation with activated T cells, spheroids were stained with <a href="/order/catalog/product/P35373#/P35373" data-uw-rm-brl="false">pHrodo Green AM Intracellular pH Indicator</a> for 30 min. The cells were then washed 3x with PBS and imaged with 4x objective using confocal on a CellInsight CX7 LZR HCS instrument. The image is a maximum intensity projection of multiple z-sections. Intracellular increase in fluorescence of pHrodo Green AM upon addition of activated T cells.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 m--learning-center-container-border mt-l1 mb-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-ca99c377f0" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/hcs/4.png" data-asset="/content/dam/tfsite/images/storefront/hcs/4.png" data-title="Imaging and analyzing proliferating cells in spheroids" id="image-1317750120" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/hcs/4.png" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Imaging and analyzing proliferating cells in spheroids" title="Imaging and analyzing proliferating cells in spheroids"/> </noscript> <meta itemprop="caption" content="Imaging and analyzing proliferating cells in spheroids"/> </div>  </div> <div class="cmp-p-text mt-l1"> <div id="text-457829330" class="cmp-text"> <p><b>Imaging and analyzing proliferating cells in spheroids</b></p> <p> </p> <p><b>Analyzing proliferating cells in HeLa spheroids.</b> HeLa cells were plated at a density of 5,000/well on a Nunclon Sphera U-bottom plate and incubated for 24 hr in a CO2 incubator. The spheroids were treated with 50 µM hydroxyurea for 24 hr. The spheroids were then pulsed with 10 µM 5-ethynyl-2’-deoxyuridine (EdU) for 30 mins. Spheroids were then washed with PBS and stained for proliferating cells using the <a href="/order/catalog/product/C10351#/C10351">Click-iT EdU Alexa Fluor 488 HCS Assay</a>. Spheroids were then washed and stained with a Ki67 antibody conjugated to Alexa Fluor 647 dye. The spheroids were automatically imaged with 10x objective using confocal on a CellInsight CX7 LZR HCS instrument. The image is a maximum intensity projection of multiple z-sections. Spheroids were segmented as an object, and EdU- and Ki67-positive cells were quantitated as puncta within the spheroid. Both EdU- and Ki67-positive cells were seen in control spheroids. In hydroxyurea-treated spheroids, the actively proliferating s-phase cells disappeared (EdU negative) and only Ki67-positive cells were observed.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 m--learning-center-container-border mt-l1 mb-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-87147bceab" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/2020/imaging-lung-cancer-spheroids-killing-penetration-T-cells-1000x392.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/2020/imaging-lung-cancer-spheroids-killing-penetration-T-cells-1000x392.jpg" data-title="Immuno-oncology application: Imaging T cell penetration and killing of lung cancer spheroids" id="image-876570241" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/2020/imaging-lung-cancer-spheroids-killing-penetration-T-cells-1000x392.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Immuno-oncology application: Imaging T cell penetration and killing of lung cancer spheroids" title="Immuno-oncology application: Imaging T cell penetration and killing of lung cancer spheroids"/> </noscript> <meta itemprop="caption" content="Immuno-oncology application: Imaging T cell penetration and killing of lung cancer spheroids"/> </div>  </div> <div class="cmp-p-text mt-l1"> <div id="text-1218328795" class="cmp-text"> <p><b>Immuno-oncology application: Imaging T cell penetration and killing of lung cancer spheroids</b></p> <p> </p> <p><b>T cell penetration and killing of lung cancer spheroids. </b>Spheroids were formed by seeding A549 cells using Gibco Minimal Essential Medium (MEM) in Nunclon Sphera U-bottom plates and culturing for 2 days. T cells isolated from human PBMCs using <a href="/order/catalog/product/11141D#/11141D" data-uw-rm-brl="false">Dynabeads Human T-Expander CD3/CD28</a> were activated for 72 hr and labeled with <a href="/order/catalog/product/C34565#/C34565" data-uw-rm-brl="false">CellTracker Deep Red Dye</a> before adding to lung cancer spheroids for 4 hr. Cells were labeled with <a href="/order/catalog/product/C10723#/C10723" data-uw-rm-brl="false">CellEvent Caspase 3/7 Reagent</a>. T cell penetration and tumor cytotoxicity were evaluated using live-cell whole-spheroid imaging on the CellInsight CX7 LZR High-Content Platform. Activated T cells penetrated the spheroids (red) and induced apoptosis in target cells throughout the spheroids as seen by increased staining with CellEvent Caspase 3/7 Reagent (green).</p> </div> </div> </div> </div> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-d317fb1b44" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> </div> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer p-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-c56a842582"> <div class="cmp-sectiontitle"> <h2 id="reference-articles" class="cmp-sectiontitle__text" data-anchor-nav-title="Reference articles">Reference articles</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container mt-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-7fa4209d94" class="cmp-container"> <div class="cmp-p-text alt"> <div id="text-724086900" class="cmp-text"> <p>Thermo Scientific high-content analysis instruments are cited extensively in reputable publications. HCA instrument citations in peer-reviewed publications are displayed here. HCA comprises a powerful combination of fluorescence microscopy, image processing, automated cellular measurements, and informatics tools that has enabled fundamental discoveries in basic research—and progression in drug compound discovery. 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