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profiling</title> <meta name="description" content="Search results for: profiling"> <meta name="keywords" content="profiling"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="profiling"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 250</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: profiling</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">250</span> A Game Theory Analysis of the Effectiveness of Passenger Profiling for Transportation Security</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yael%20Deutsch">Yael Deutsch</a>, <a href="https://publications.waset.org/abstracts/search?q=Arieh%20Gavious"> Arieh Gavious</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The threat of aviation terrorism and its potential damage became significant after the 9/11 terror attacks. These attacks have led authorities and leaders to suggest that security personnel should overcome politically correct scruples about profiling and use it openly. However, there is a lack of knowledge about the smart usage of profiling and its advantages. We analyze game models that are suitable to specific real-world scenarios, focusing on profiling as a tool to detect potential violators, such as terrorists and smugglers. We provide analytical and clear answers to difficult questions, and by that help fighting against harmful violation acts. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=game%20theory" title="game theory">game theory</a>, <a href="https://publications.waset.org/abstracts/search?q=profiling" title=" profiling"> profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=security" title=" security"> security</a>, <a href="https://publications.waset.org/abstracts/search?q=nash%20equilibrium" title=" nash equilibrium"> nash equilibrium</a> </p> <a href="https://publications.waset.org/abstracts/150701/a-game-theory-analysis-of-the-effectiveness-of-passenger-profiling-for-transportation-security" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/150701.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">109</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">249</span> Authorship Attribution Using Sociolinguistic Profiling When Considering Civil and Criminal Cases</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Diana%20A.%20Sokolova">Diana A. Sokolova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This article is devoted to one of the possibilities for identifying the author of an oral or written text - sociolinguistic profiling. Sociolinguistic profiling is utilized as a forensic linguistics technique to identify individuals through language patterns, particularly in criminal cases. It examines how social factors influence language use. This study aims to showcase the significance of linguistic profiling for attributing authorship in texts and emphasizes the necessity for its continuous enhancement while considering its strengths and weaknesses. The study employs semantic-syntactic, lexical-semantic, linguopragmatic, logical, presupposition, authorization, and content analysis methods to investigate linguistic profiling. The research highlights the relevance of sociolinguistic profiling in authorship attribution and underscores the importance of ongoing refinement of the technique, considering its limitations. This study emphasizes the practical application of linguistic profiling in legal settings and underscores the impact of social factors on language use, contributing to the field of forensic linguistics. Data collection involves collecting oral and written texts from criminal and civil court cases to analyze language patterns for authorship attribution. The collected data is analyzed using various linguistic analysis methods to identify individual characteristics and patterns that can aid in authorship attribution. The study addresses the effectiveness of sociolinguistic profiling in identifying authors of texts and explores the impact of social factors on language use in legal contexts. In spite of advantages challenges in linguistics profiling have spurred debates and controversies in academic circles, legal environments, and the public sphere. So, this research highlights the significance of sociolinguistic profiling in authorship attribution and emphasizes the need for further development of this method, considering its strengths and weaknesses. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=authorship%20attribution" title="authorship attribution">authorship attribution</a>, <a href="https://publications.waset.org/abstracts/search?q=detection%20of%20identifying" title=" detection of identifying"> detection of identifying</a>, <a href="https://publications.waset.org/abstracts/search?q=dialect" title=" dialect"> dialect</a>, <a href="https://publications.waset.org/abstracts/search?q=features" title=" features"> features</a>, <a href="https://publications.waset.org/abstracts/search?q=forensic%20linguistics" title=" forensic linguistics"> forensic linguistics</a>, <a href="https://publications.waset.org/abstracts/search?q=social%20influence" title=" social influence"> social influence</a>, <a href="https://publications.waset.org/abstracts/search?q=sociolinguistics" title=" sociolinguistics"> sociolinguistics</a>, <a href="https://publications.waset.org/abstracts/search?q=unique%20speech%20characteristics" title=" unique speech characteristics"> unique speech characteristics</a> </p> <a href="https://publications.waset.org/abstracts/188208/authorship-attribution-using-sociolinguistic-profiling-when-considering-civil-and-criminal-cases" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188208.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">37</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">248</span> Consumer Load Profile Determination with Entropy-Based K-Means Algorithm</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ioannis%20P.%20Panapakidis">Ioannis P. Panapakidis</a>, <a href="https://publications.waset.org/abstracts/search?q=Marios%20N.%20Moschakis"> Marios N. Moschakis</a> </p> <p class="card-text"><strong>Abstract:</strong></p> With the continuous increment of smart meter installations across the globe, the need for processing of the load data is evident. Clustering-based load profiling is built upon the utilization of unsupervised machine learning tools for the purpose of formulating the typical load curves or load profiles. The most commonly used algorithm in the load profiling literature is the K-means. While the algorithm has been successfully tested in a variety of applications, its drawback is the strong dependence in the initialization phase. This paper proposes a novel modified form of the K-means that addresses the aforementioned problem. Simulation results indicate the superiority of the proposed algorithm compared to the K-means. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=clustering" title="clustering">clustering</a>, <a href="https://publications.waset.org/abstracts/search?q=load%20profiling" title=" load profiling"> load profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=load%20modeling" title=" load modeling"> load modeling</a>, <a href="https://publications.waset.org/abstracts/search?q=machine%20learning" title=" machine learning"> machine learning</a>, <a href="https://publications.waset.org/abstracts/search?q=energy%20efficiency%20and%20quality" title=" energy efficiency and quality"> energy efficiency and quality</a> </p> <a href="https://publications.waset.org/abstracts/89525/consumer-load-profile-determination-with-entropy-based-k-means-algorithm" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/89525.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">164</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">247</span> Time-Series Load Data Analysis for User Power Profiling</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mahdi%20Daghmhehci%20Firoozjaei">Mahdi Daghmhehci Firoozjaei</a>, <a href="https://publications.waset.org/abstracts/search?q=Minchang%20Kim"> Minchang Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Dima%20Alhadidi"> Dima Alhadidi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, we present a power profiling model for smart grid consumers based on real time load data acquired smart meters. It profiles consumers’ power consumption behaviour using the dynamic time warping (DTW) clustering algorithm. Due to the invariability of signal warping of this algorithm, time-disordered load data can be profiled and consumption features be extracted. Two load types are defined and the related load patterns are extracted for classifying consumption behaviour by DTW. The classification methodology is discussed in detail. To evaluate the performance of the method, we analyze the time-series load data measured by a smart meter in a real case. The results verify the effectiveness of the proposed profiling method with 90.91% true positive rate for load type clustering in the best case. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=power%20profiling" title="power profiling">power profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=user%20privacy" title=" user privacy"> user privacy</a>, <a href="https://publications.waset.org/abstracts/search?q=dynamic%20time%20warping" title=" dynamic time warping"> dynamic time warping</a>, <a href="https://publications.waset.org/abstracts/search?q=smart%20grid" title=" smart grid"> smart grid</a> </p> <a href="https://publications.waset.org/abstracts/153678/time-series-load-data-analysis-for-user-power-profiling" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/153678.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">149</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">246</span> Identifying Common Behavioural Traits of Lone-Wolves in Recent Terrorist Attacks in Europe</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Khaled%20M.%20Khan">Khaled M. Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Armstrong%20Nhlabatsi"> Armstrong Nhlabatsi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This article attempts to analyse behavioural traits of lone-wolves who struck and killed innocents in six different attacks in Europe in last nine months. The main objective of this study is to develop a profiling template in order to capture commonality of characteristics of these attackers. This study tries to understand the homogeneity of lone-wolves in terms of their social background and state of mind. The commonality among them can possibly be used to build a profiling template that could help detecting vulnerable persons who are prone to be self-radicalised or radicalised by someone else. The result of this study provides us an understanding of their commonality in terms of their state of mind and social characteristics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=behavioral%20pattern" title="behavioral pattern">behavioral pattern</a>, <a href="https://publications.waset.org/abstracts/search?q=terrorism" title=" terrorism"> terrorism</a>, <a href="https://publications.waset.org/abstracts/search?q=profiling" title=" profiling"> profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=commonality" title=" commonality"> commonality</a> </p> <a href="https://publications.waset.org/abstracts/72948/identifying-common-behavioural-traits-of-lone-wolves-in-recent-terrorist-attacks-in-europe" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72948.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">402</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">245</span> Predicting Destination Station Based on Public Transit Passenger Profiling</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xuyang%20Song">Xuyang Song</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun%20Yin"> Jun Yin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The smart card has been an extremely universal tool in public transit. It collects a large amount of data on buses, urban railway transit, and ferries and provides possibilities for passenger profiling. This paper combines offline analysis of passenger profiling and real-time prediction to propose a method that can accurately predict the destination station in real-time when passengers tag on. Firstly, this article constructs a static database of user travel characteristics after identifying passenger travel patterns based on the Density-Based Spatial Clustering of Applications with Noise (DBSCAN). The dual travel passenger habits are identified: OD travel habits and D station travel habits. Then a rapid real-time prediction algorithm based on Transit Passenger Profiling is proposed, which can predict the destination of in-board passengers. This article combines offline learning with online prediction, providing a technical foundation for real-time passenger flow prediction, monitoring and simulation, and short-term passenger behavior and demand prediction. This technology facilitates the efficient and real-time acquisition of passengers' travel destinations and demand. The last, an actual case was simulated and demonstrated feasibility and efficiency. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=travel%20behavior" title="travel behavior">travel behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=destination%20prediction" title=" destination prediction"> destination prediction</a>, <a href="https://publications.waset.org/abstracts/search?q=public%20transit" title=" public transit"> public transit</a>, <a href="https://publications.waset.org/abstracts/search?q=passenger%20profiling" title=" passenger profiling"> passenger profiling</a> </p> <a href="https://publications.waset.org/abstracts/190243/predicting-destination-station-based-on-public-transit-passenger-profiling" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/190243.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">19</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">244</span> Applying Massively Parallel Sequencing to Forensic Soil Bacterial Profiling</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hui%20Li">Hui Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Xueying%20Zhao"> Xueying Zhao</a>, <a href="https://publications.waset.org/abstracts/search?q=Ke%20Ma"> Ke Ma</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu%20Cao"> Yu Cao</a>, <a href="https://publications.waset.org/abstracts/search?q=Fan%20Yang"> Fan Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Qingwen%20Xu"> Qingwen Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Wenbin%20Liu"> Wenbin Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Soil can often link a person or item to a crime scene, which makes it a valuable evidence in forensic casework. Several techniques have been utilized in forensic soil discrimination in previous studies. Because soil contains a vast number of microbiomes, the analyse of soil microbiomes is expected to be a potential way to characterise soil evidence. In this study, we applied massively parallel sequencing (MPS) to soil bacterial profiling on the Ion Torrent Personal Genome Machine (PGM). Soils from different regions were collected repeatedly. V-region 3 and 4 of Bacterial 16S rRNA gene were detected by MPS. Operational taxonomic units (OTU, 97%) were used to analyse soil bacteria. Several bioinformatics methods (PCoA, NMDS, Metastats, LEfse, and Heatmap) were applied in bacterial profiles. Our results demonstrate that MPS can provide a more detailed picture of the soil microbiomes and the composition of soil bacterial components from different region was individualistic. In conclusion, the utility of soil bacterial profiling via MPS of the 16S rRNA gene has potential value in characterising soil evidences and associating them with their place of origin, which can play an important role in forensic science in the future. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacterial%20profiling" title="bacterial profiling">bacterial profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=forensic" title=" forensic"> forensic</a>, <a href="https://publications.waset.org/abstracts/search?q=massively%20parallel%20sequencing" title=" massively parallel sequencing"> massively parallel sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20evidence" title=" soil evidence"> soil evidence</a> </p> <a href="https://publications.waset.org/abstracts/80561/applying-massively-parallel-sequencing-to-forensic-soil-bacterial-profiling" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80561.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">563</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">243</span> Physicians’ Knowledge and Perception of Gene Profiling in Malaysia: A Pilot Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Farahnaz%20Amini">Farahnaz Amini</a>, <a href="https://publications.waset.org/abstracts/search?q=Woo%20Yun%20Kin"> Woo Yun Kin</a>, <a href="https://publications.waset.org/abstracts/search?q=Lazwani%20Kolandaiveloo"> Lazwani Kolandaiveloo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Availability of different genetic tests after completion of Human Genome Project increases the physicians’ responsibility to keep themselves update on the potential implementation of these genetic tests in their daily practice. However, due to numbers of barriers, still many of physicians are not either aware of these tests or are not willing to offer or refer their patients for genetic tests. This study was conducted an anonymous, cross-sectional, mailed-based survey to develop a primary data of Malaysian physicians’ level of knowledge and perception of gene profiling. Questionnaire had 29 questions. Total scores on selected questions were used to assess the level of knowledge. The highest possible score was 11. Descriptive statistics, one way ANOVA and chi-squared test was used for statistical analysis. Sixty three completed questionnaires was returned by 27 general practitioners (GPs) and 36 medical specialists. Responders’ age range from 24 to 55 years old (mean 30.2 ± 6.4). About 40% of the participants rated themselves as having poor level of knowledge in genetics in general whilst 60% believed that they have fair level of knowledge. However, almost half (46%) of the respondents felt that they were not knowledgeable about available genetic tests. A majority (94%) of the responders were not aware of any lab or company which is offering gene profiling services in Malaysia. Only 4% of participants were aware of using gene profiling for detection of dosage of some drugs. Respondents perceived greater utility of gene profiling for breast cancer (38%) compared to the colorectal familial cancer (3%). The score of knowledge ranged from 2 to 8 (mean 4.38 ± 1.67). Non-significant differences between score of knowledge of GPs and specialists were observed, with score of 4.19 and 4.58 respectively. There was no significant association between any demographic factors and level of knowledge. However, those who graduated between years 2001 to 2005 had higher level of knowledge. Overall, 83% of participants showed relatively high level of perception on value of gene profiling to detect patient’s risk of disease. However, low perception was observed for both statements of using gene profiling for general population in order to alter their lifestyle (25%) as well as having the full sequence of a patient genome for the purpose of determining a patient’s best match for treatment (18%). The lack of clinical guidelines, limited provider knowledge and awareness, lack of time and resources to educate patients, lack of evidence-based clinical information and cost of tests were the most barriers of ordering gene profiling mentioned by physicians. In conclusion Malaysian physicians who participate in this study had mediocre level of knowledge and awareness in gene profiling. The low exposure to the genetic questions and problems might be a key predictor of lack of awareness and knowledge on available genetic tests. Educational and training workshop might be useful in helping Malaysian physicians incorporate genetic profiling into practice for eligible patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gene%20profiling" title="gene profiling">gene profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=knowledge" title=" knowledge"> knowledge</a>, <a href="https://publications.waset.org/abstracts/search?q=Malaysia" title=" Malaysia"> Malaysia</a>, <a href="https://publications.waset.org/abstracts/search?q=physician" title=" physician"> physician</a> </p> <a href="https://publications.waset.org/abstracts/11902/physicians-knowledge-and-perception-of-gene-profiling-in-malaysia-a-pilot-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/11902.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">326</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">242</span> An International Comparison of Forensic Identification Evidence Legislation: Balancing Community Interests and Individual Rights</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marcus%20Smith">Marcus Smith</a> </p> <p class="card-text"><strong>Abstract:</strong></p> DNA profiling has made a valuable contribution to criminal investigations over the past thirty years. Direct matching DNA profiles from a crime scene and suspect, or between a suspect and a database remain of great importance to crimes such as murder, assault, and property theft. As scientific and technological advancement continues, a wide range of new DNA profiling applications has been developed. The application of new techniques involves an interesting balancing act between admitting probative evidence in a criminal trial, evaluating its degree of relevance and validity, and limiting its prejudicial impact. The impact of new DNA profiling applications that have significant implications for law enforcement and the legal system can be evaluated through a review of relevant case law, legislation and the latest empirical evidence from jurisdictions around the world including the United States, United Kingdom, and Australia. There are benefits in further examining the implications of these new developments, including how the criminal law can best be adapted to ensure that new technology is used to enhance criminal investigation and prosecution while ensuring it is applied in a measured way that respects individual rights and maintains principles of fairness enshrined in the legal system. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=criminal%20procedure" title="criminal procedure">criminal procedure</a>, <a href="https://publications.waset.org/abstracts/search?q=forensic%20evidence" title=" forensic evidence"> forensic evidence</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20profiling" title=" DNA profiling"> DNA profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=familial%20searching" title=" familial searching"> familial searching</a>, <a href="https://publications.waset.org/abstracts/search?q=phenotyping" title=" phenotyping"> phenotyping</a> </p> <a href="https://publications.waset.org/abstracts/83037/an-international-comparison-of-forensic-identification-evidence-legislation-balancing-community-interests-and-individual-rights" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/83037.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">132</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">241</span> lncRNA Gene Expression Profiling Analysis by TCGA RNA-Seq Data of Breast Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xiaoping%20Su">Xiaoping Su</a>, <a href="https://publications.waset.org/abstracts/search?q=Gabriel%20G.%20Malouf"> Gabriel G. Malouf</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Breast cancer is a heterogeneous disease that can be classified in 4 subgroups using transcriptional profiling. The role of lncRNA expression in human breast cancer biology, prognosis, and molecular classification remains unknown. Methods and results: Using an integrative comprehensive analysis of lncRNA, mRNA and DNA methylation in 900 breast cancer patients from The Cancer Genome Atlas (TCGA) project, we unraveled the molecular portraits of 1,700 expressed lncRNA. Some of those lncRNAs (i.e, HOTAIR) are previously reported and others are novel (i.e, HOTAIRM1, MAPT-AS1). The lncRNA classification correlated well with the PAM50 classification for basal-like, Her-2 enriched and luminal B subgroups, in contrast to the luminal A subgroup which behaved differently. Importantly, estrogen receptor (ESR1) expression was associated with distinct lncRNA networks in lncRNA clusters III and IV. Gene set enrichment analysis for cis- and trans-acting lncRNA showed enrichment for breast cancer signatures driven by breast cancer master regulators. Almost two third of those lncRNA were marked by enhancer chromatin modifications (i.e., H3K27ac), suggesting that lncRNA expression may result in increased activity of neighboring genes. Differential analysis of gene expression profiling data showed that lncRNA HOTAIRM1 was significantly down-regulated in basal-like subtype, and DNA methylation profiling data showed that lncRNA HOTAIRM1 was highly methylated in basal-like subtype. Thus, our integrative analysis of gene expression and DNA methylation strongly suggested that lncRNA HOTAIRM1 should be a tumor suppressor in basal-like subtype. Conclusion and significance: Our study depicts the first lncRNA molecular portrait of breast cancer and shows that lncRNA HOTAIRM1 might be a novel tumor suppressor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lncRNA%20profiling" title="lncRNA profiling">lncRNA profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title=" breast cancer"> breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=HOTAIRM1" title=" HOTAIRM1"> HOTAIRM1</a>, <a href="https://publications.waset.org/abstracts/search?q=tumor%20suppressor" title=" tumor suppressor"> tumor suppressor</a> </p> <a href="https://publications.waset.org/abstracts/104472/lncrna-gene-expression-profiling-analysis-by-tcga-rna-seq-data-of-breast-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104472.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">105</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">240</span> The Effects of the “War on Drugs” on Black and Latino Youth</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aniya%20Everette">Aniya Everette</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In June of 1971, President Richard Nixon made his first public declaration regarding the “War on Drugs,” deeming it America’s number one public enemy. Since then, the drug war has been a divisive topic in the US. Black and brown children have been unfairly targeted and imprisoned at alarming rates as a result of this policy, which has had a negative effect on them. Black and Latino youth have faced significant obstacles that have impacted their educational opportunities, employment prospects, and general quality of life due to harsh mandatory sentences, aggressive policing tactics, and racial profiling. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=war" title="war">war</a>, <a href="https://publications.waset.org/abstracts/search?q=drugs" title=" drugs"> drugs</a>, <a href="https://publications.waset.org/abstracts/search?q=crime" title=" crime"> crime</a>, <a href="https://publications.waset.org/abstracts/search?q=racial%20profiling" title=" racial profiling"> racial profiling</a> </p> <a href="https://publications.waset.org/abstracts/166787/the-effects-of-the-war-on-drugs-on-black-and-latino-youth" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/166787.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">82</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">239</span> An Unbiased Profiling of Immune Repertoire via Sequencing and Analyzing T-Cell Receptor Genes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yi-Lin%20Chen">Yi-Lin Chen</a>, <a href="https://publications.waset.org/abstracts/search?q=Sheng-Jou%20Hung"> Sheng-Jou Hung</a>, <a href="https://publications.waset.org/abstracts/search?q=Tsunglin%20Liu"> Tsunglin Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Adaptive immune system recognizes a wide range of antigens via expressing a large number of structurally distinct T cell and B cell receptor genes. The distinct receptor genes arise from complex rearrangements called V(D)J recombination, and constitute the immune repertoire. A common method of profiling immune repertoire is via amplifying recombined receptor genes using multiple primers and high-throughput sequencing. This multiplex-PCR approach is efficient; however, the resulting repertoire can be distorted because of primer bias. To eliminate primer bias, 5’ RACE is an alternative amplification approach. However, the application of RACE approach is limited by its low efficiency (i.e., the majority of data are non-regular receptor sequences, e.g., containing intronic segments) and lack of the convenient tool for analysis. We propose a computational tool that can correctly identify non-regular receptor sequences in RACE data via aligning receptor sequences against the whole gene instead of only the exon regions as done in all other tools. Using our tool, the remaining regular data allow for an accurate profiling of immune repertoire. In addition, a RACE approach is improved to yield a higher fraction of regular T-cell receptor sequences. Finally, we quantify the degree of primer bias of a multiplex-PCR approach via comparing it to the RACE approach. The results reveal significant differences in frequency of VJ combination by the two approaches. Together, we provide a new experimental and computation pipeline for an unbiased profiling of immune repertoire. As immune repertoire profiling has many applications, e.g., tracing bacterial and viral infection, detection of T cell lymphoma and minimal residual disease, monitoring cancer immunotherapy, etc., our work should benefit scientists who are interested in the applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=immune%20repertoire" title="immune repertoire">immune repertoire</a>, <a href="https://publications.waset.org/abstracts/search?q=T-cell%20receptor" title=" T-cell receptor"> T-cell receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=5%27%20RACE" title=" 5' RACE"> 5' RACE</a>, <a href="https://publications.waset.org/abstracts/search?q=high-throughput%20sequencing" title=" high-throughput sequencing"> high-throughput sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=sequence%20alignment" title=" sequence alignment"> sequence alignment</a> </p> <a href="https://publications.waset.org/abstracts/88972/an-unbiased-profiling-of-immune-repertoire-via-sequencing-and-analyzing-t-cell-receptor-genes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88972.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">194</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">238</span> Pattern Recognition Approach Based on Metabolite Profiling Using In vitro Cancer Cell Line</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Amanina%20Iymia%20Jeffree">Amanina Iymia Jeffree</a>, <a href="https://publications.waset.org/abstracts/search?q=Reena%20Thriumani"> Reena Thriumani</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Iqbal%20Omar"> Mohammad Iqbal Omar</a>, <a href="https://publications.waset.org/abstracts/search?q=Ammar%20Zakaria"> Ammar Zakaria</a>, <a href="https://publications.waset.org/abstracts/search?q=Yumi%20Zuhanis%20Has-Yun%20Hashim"> Yumi Zuhanis Has-Yun Hashim</a>, <a href="https://publications.waset.org/abstracts/search?q=Ali%20Yeon%20Md%20Shakaff"> Ali Yeon Md Shakaff</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Metabolite profiling is a strategy to be approached in the pattern recognition method focused on three types of cancer cell line that driving the most to death specifically lung, breast, and colon cancer. The purpose of this study was to discriminate the VOCs pattern among cancerous and control group based on metabolite profiling. The sampling was executed utilizing the cell culture technique. All culture flasks were incubated till 72 hours and data collection started after 24 hours. Every running sample took 24 minutes to be completed accordingly. The comparative metabolite patterns were identified by the implementation of headspace-solid phase micro-extraction (HS-SPME) sampling coupled with gas chromatography-mass spectrometry (GCMS). The optimizations of the main experimental variables such as oven temperature and time were evaluated by response surface methodology (RSM) to get the optimal condition. Volatiles were acknowledged through the National Institute of Standards and Technology (NIST) mass spectral database and retention time libraries. To improve the reliability of significance, it is of crucial importance to eliminate background noise which data from 3rd minutes to 17th minutes were selected for statistical analysis. Targeted metabolites, of which were annotated as known compounds with the peak area greater than 0.5 percent were highlighted and subsequently treated statistically. Volatiles produced contain hundreds to thousands of compounds; therefore, it will be optimized by chemometric analysis, such as principal component analysis (PCA) as a preliminary analysis before subjected to a pattern classifier for identification of VOC samples. The volatile organic compound profiling has shown to be significantly distinguished among cancerous and control group based on metabolite profiling. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20cancer%20cell%20line" title="in vitro cancer cell line">in vitro cancer cell line</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolite%20profiling" title=" metabolite profiling"> metabolite profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=pattern%20recognition" title=" pattern recognition"> pattern recognition</a>, <a href="https://publications.waset.org/abstracts/search?q=volatile%20organic%20compounds" title=" volatile organic compounds"> volatile organic compounds</a> </p> <a href="https://publications.waset.org/abstracts/66519/pattern-recognition-approach-based-on-metabolite-profiling-using-in-vitro-cancer-cell-line" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/66519.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">367</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">237</span> DNA as an Instrument in Constructing Narratives and Justice in Criminal Investigations: A Socio-Epistemological Exploration</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aadita%20Chaudhury">Aadita Chaudhury</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Since at least the early 2000s, DNA profiling has achieved a preeminent status in forensic investigations into criminal acts. While the criminal justice system has a long history of using forensic evidence and testing them through establish technoscientific means, the primacy of DNA in establishing 'truth' or reconstructing a series of events is unparalleled in the history of forensic science. This paper seeks to elucidate the ways in which DNA profiling has become the most authoritative instrument of 'truth' in criminal investigations, and how it is used in the legal process to ascertain culpability, create the notion of infallible evidence, and advance the search for justice. It is argued that DNA profiling has created a paradigm shift in how the legal system and the general public understands crime and culpability, but not without limitations. There are indications that even trace amounts of DNA evidence can point to causal links in a criminal investigation, however, there still remains many rooms to create confusion and doubt from empirical evidence within the narrative of crimes. Many of the shortcomings of DNA-based forensic investigations are explored and evaluated with regards to claims of the authority of biological evidence and implications for the public understanding of the elusive concepts of truth and justice in the present era. Public misinformation about the forensic analysis processes could produce doubt or faith in the judgements rooted in them, depending on other variables presented at the trial. A positivist understanding of forensic science that is shared by the majority of the population does not take into consideration that DNA evidence is far from definitive, and can be used to support any theories of culpability, to create doubt and to deflect blame. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DNA%20profiling" title="DNA profiling">DNA profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=epistemology%20of%20forensic%20science" title=" epistemology of forensic science"> epistemology of forensic science</a>, <a href="https://publications.waset.org/abstracts/search?q=philosophy%20of%20forensic%20science" title=" philosophy of forensic science"> philosophy of forensic science</a>, <a href="https://publications.waset.org/abstracts/search?q=sociology%20of%20scientific%20knowledge" title=" sociology of scientific knowledge"> sociology of scientific knowledge</a> </p> <a href="https://publications.waset.org/abstracts/70471/dna-as-an-instrument-in-constructing-narratives-and-justice-in-criminal-investigations-a-socio-epistemological-exploration" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/70471.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">208</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">236</span> Authorship Profiling of Unidentified Corpora in Saudi Social Media</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdulaziz%20Fageeh">Abdulaziz Fageeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the bustling digital landscape of Saudi Arabia, a chilling wave of cybercrime has swept across the nation. Among the most nefarious acts are financial blackmail schemes, perpetrated by anonymous actors lurking within the shadows of social media platforms. This chilling reality necessitates the utilization of forensic linguistic techniques to unravel the identities of these virtual villains. This research delves into the complex world of authorship profiling, investigating the effectiveness of various linguistic features in identifying the perpetrators of malicious messages within the Saudi social media environment. By meticulously analyzing patterns of language, vocabulary choice, and stylistic nuances, the study endeavors to uncover the hidden characteristics of the individuals responsible for these heinous acts. Through this linguistic detective work, the research aims to provide valuable insights to investigators and policymakers in the ongoing battle against cybercrime and to shed light on the evolution of malicious online behavior within the Saudi context. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=authorship%20profiling" title="authorship profiling">authorship profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=arabic%20linguistics" title=" arabic linguistics"> arabic linguistics</a>, <a href="https://publications.waset.org/abstracts/search?q=saudi%20social%20media" title=" saudi social media"> saudi social media</a>, <a href="https://publications.waset.org/abstracts/search?q=cybercrime" title=" cybercrime"> cybercrime</a>, <a href="https://publications.waset.org/abstracts/search?q=financial%20blackmail" title=" financial blackmail"> financial blackmail</a>, <a href="https://publications.waset.org/abstracts/search?q=linguistic%20features" title=" linguistic features"> linguistic features</a>, <a href="https://publications.waset.org/abstracts/search?q=forensic%20linguistics" title=" forensic linguistics"> forensic linguistics</a>, <a href="https://publications.waset.org/abstracts/search?q=online%20threats" title=" online threats"> online threats</a> </p> <a href="https://publications.waset.org/abstracts/193123/authorship-profiling-of-unidentified-corpora-in-saudi-social-media" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193123.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">12</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">235</span> Qualitative Profiling in Practice: The Italian Public Employment Services Experience</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=L.%20Agneni">L. Agneni</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Carta"> F. Carta</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Micheletta"> C. Micheletta</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Tersigni"> V. Tersigni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The development of a qualitative method to profile jobseekers is needed to improve the quality of the Public Employment Services (PES) in Italy. This is why the National Agency for Active Labour Market Policies (ANPAL) decided to introduce a Qualitative Profiling Service in the context of the activities carried out by local employment offices’ operators. The qualitative profiling service provides information and data regarding the jobseeker’s personal transition status, through a semi-structured questionnaire administered to PES clients during the guidance interview. The questionnaire responses allow PES staff to identify, for each client, proper activities and policy measures to support jobseekers in their reintegration into the labour market. Data and information gathered by the qualitative profiling tool are the following: frequency, modalities and motivations for clients to apply to local employment offices; clients’ expectations and skills; difficulties that they have faced during the previous working experiences; strategies, actions undertaken and activated channels for job search. These data are used to assess jobseekers’ personal and career characteristics and to measure their employability level (qualitative profiling index), in order to develop and deliver tailor-made action programmes for each client. This paper illustrates the use of the above-mentioned qualitative profiling service on the national territory and provides an overview of the main findings of the survey: concerning the difficulties that unemployed people face in finding a job and their perception of different aspects related to the transition in the labour market. The survey involved over 10.000 jobseekers registered with the PES. Most of them are beneficiaries of the “citizens' income”, a specific active labour policy and social inclusion measure. Furthermore, data analysis allows classifying jobseekers into a specific group of clients with similar features and behaviours, on the basis of socio-demographic variables, customers' expectations, needs and required skills for the profession for which they seek employment. Finally, the survey collects PES staff opinions and comments concerning clients’ difficulties in finding a new job and also their strengths. This is a starting point for PESs’ operators to define adequate strategies to facilitate jobseekers’ access or reintegration into the labour market. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=labour%20market%20transition" title="labour market transition">labour market transition</a>, <a href="https://publications.waset.org/abstracts/search?q=public%20employment%20services" title=" public employment services"> public employment services</a>, <a href="https://publications.waset.org/abstracts/search?q=qualitative%20profiling" title=" qualitative profiling"> qualitative profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=vocational%20guidance" title=" vocational guidance"> vocational guidance</a> </p> <a href="https://publications.waset.org/abstracts/128153/qualitative-profiling-in-practice-the-italian-public-employment-services-experience" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/128153.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">140</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">234</span> Critical Core Skills Profiling in the Singaporean Workforce</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bi%20Xiao%20Fang">Bi Xiao Fang</a>, <a href="https://publications.waset.org/abstracts/search?q=Tan%20Bao%20Zhen"> Tan Bao Zhen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Soft skills, core competencies, and generic competencies are exchangeable terminologies often used to represent a similar concept. In the Singapore context, such skills are currently being referred to as Critical Core Skills (CCS). In 2019, SkillsFuture Singapore (SSG) reviewed the Generic Skills and Competencies (GSC) framework that was first introduced in 2016, culminating in the development of the Critical Core Skills (CCS) framework comprising 16 soft skills classified into three clusters. The CCS framework is part of the Skills Framework, and whose stated purpose is to create a common skills language for individuals, employers and training providers. It is also developed with the objectives of building deep skills for a lean workforce, enhance business competitiveness and support employment and employability. This further helps to facilitate skills recognition and support the design of training programs for skills and career development. According to SSG, every job role requires a set of technical skills and a set of Critical Core Skills to perform well at work, whereby technical skills refer to skills required to perform key tasks of the job. There has been an increasing emphasis on soft skills for the future of work. A recent study involving approximately 80 organizations across 28 sectors in Singapore revealed that more enterprises are beginning to recognize that soft skills support their employees’ performance and business competitiveness. Though CCS is of high importance for the development of the workforce’s employability, there is little attention paid to the CCS use and profiling across occupations. A better understanding of how CCS is distributed across the economy will thus significantly enhance SSG’s career guidance services as well as training providers’ services to graduates and workers and guide organizations in their hiring for soft skills. This CCS profiling study sought to understand how CCS is demanded in different occupations. To achieve its research objectives, this study adopted a quantitative method to measure CCS use across different occupations in the Singaporean workforce. Based on the CCS framework developed by SSG, the research team adopted a formative approach to developing the CCS profiling tool to measure the importance of and self-efficacy in the use of CCS among the Singaporean workforce. Drawing on the survey results from 2500 participants, this study managed to profile them into seven occupation groups based on the different patterns of importance and confidence levels of the use of CCS. Each occupation group is labeled according to the most salient and demanded CCS. In the meantime, the CCS in each occupation group, which may need some further strengthening, were also identified. The profiling of CCS use has significant implications for different stakeholders, e.g., employers could leverage the profiling results to hire the staff with the soft skills demanded by the job. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=employability" title="employability">employability</a>, <a href="https://publications.waset.org/abstracts/search?q=skills%20profiling" title=" skills profiling"> skills profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=skills%20measurement" title=" skills measurement"> skills measurement</a>, <a href="https://publications.waset.org/abstracts/search?q=soft%20skills" title=" soft skills"> soft skills</a> </p> <a href="https://publications.waset.org/abstracts/151108/critical-core-skills-profiling-in-the-singaporean-workforce" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/151108.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">95</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">233</span> Web-GIS Technology: A Tool for Farm-to-Market Road Project Profiling and Proposal Prioritization of the Philippines’ Department of Agriculture</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Elbert%20S.%20Moyon">Elbert S. Moyon</a>, <a href="https://publications.waset.org/abstracts/search?q=Edsel%20Matt%20O.%20Morales"> Edsel Matt O. Morales</a>, <a href="https://publications.waset.org/abstracts/search?q=Jaymer%20M.%20Jayoma"> Jaymer M. Jayoma</a>, <a href="https://publications.waset.org/abstracts/search?q=Kent%20C.%20Espejon"> Kent C. Espejon</a>, <a href="https://publications.waset.org/abstracts/search?q=Jayson%20C.%20Dollete"> Jayson C. Dollete</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20Phil%20B.%20Pacot"> Mark Phil B. Pacot</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This research paper focuses on the potential of using Web-GIS technology in prioritizing farm-to-market road projects by the Philippines’ Department of Agriculture (DA). The study aimed to explore the benefits of Web-GIS in addressing the limitations faced by the DA in terms of Farm to Market Road profiling and project prioritization, which include a lack of access to updated data, limited spatial analysis capabilities, and difficulties in sharing information between stakeholders. The research methodology involves a comprehensive literature review and a case study of a Web-GIS application developed for the DA, which was used to profile and prioritize farm-to-market road projects in the Philippines. The results showed that the Web-GIS technology provides the DA with an effective tool for analyzing and visualizing data, which can help in profiling and prioritizing road projects based on various criteria such as economic, social, and environmental impacts. The study also showed that Web-GIS technology could help in reducing the time and effort required for road project prioritization and improve communication between stakeholders. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=GIS" title="GIS">GIS</a>, <a href="https://publications.waset.org/abstracts/search?q=web%20application" title=" web application"> web application</a>, <a href="https://publications.waset.org/abstracts/search?q=farm-to-market%20road" title=" farm-to-market road"> farm-to-market road</a>, <a href="https://publications.waset.org/abstracts/search?q=FMR%20prioritization" title=" FMR prioritization"> FMR prioritization</a>, <a href="https://publications.waset.org/abstracts/search?q=Django" title=" Django"> Django</a>, <a href="https://publications.waset.org/abstracts/search?q=GeoServer" title=" GeoServer"> GeoServer</a> </p> <a href="https://publications.waset.org/abstracts/163890/web-gis-technology-a-tool-for-farm-to-market-road-project-profiling-and-proposal-prioritization-of-the-philippines-department-of-agriculture" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/163890.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">83</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">232</span> Using Multiomic Plasma Profiling From Liquid Biopsies to Identify Potential Signatures for Disease Diagnostics in Late-Stage Non-small Cell Lung Cancer (NSCLC) in Trinidad and Tobago</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nicole%20Ramlachan">Nicole Ramlachan</a>, <a href="https://publications.waset.org/abstracts/search?q=Samuel%20Mark%20West"> Samuel Mark West</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lung cancer is the leading cause of cancer-associated deaths in North America, with the vast majority being non-small cell lung cancer (NSCLC), with a five-year survival rate of only 24%. Non-invasive discovery of biomarkers associated with early-diagnosis of NSCLC can enable precision oncology efforts using liquid biopsy-based multiomics profiling of plasma. Although tissue biopsies are currently the gold standard for tumor profiling, this method presents many limitations since these are invasive, risky, and sometimes hard to obtain as well as only giving a limited tumor profile. Blood-based tests provides a less-invasive, more robust approach to interrogate both tumor- and non-tumor-derived signals. We intend to examine 30 stage III-IV NSCLC patients pre-surgery and collect plasma samples.Cell-free DNA (cfDNA) will be extracted from plasma, and next-generation sequencing (NGS) performed. Through the analysis of tumor-specific alterations, including single nucleotide variants (SNVs), insertions, deletions, copy number variations (CNVs), and methylation alterations, we intend to identify tumor-derived DNA—ctDNA among the total pool of cfDNA. This would generate data to be used as an accurate form of cancer genotyping for diagnostic purposes. Using liquid biopsies offer opportunities to improve the surveillance of cancer patients during treatment and would supplement current diagnosis and tumor profiling strategies previously not readily available in Trinidad and Tobago. It would be useful and advantageous to use this in diagnosis and tumour profiling as well as to monitor cancer patients, providing early information regarding disease evolution and treatment efficacy, and reorient treatment strategies in, timethereby improving clinical oncology outcomes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=genomics" title="genomics">genomics</a>, <a href="https://publications.waset.org/abstracts/search?q=multiomics" title=" multiomics"> multiomics</a>, <a href="https://publications.waset.org/abstracts/search?q=clinical%20genetics" title=" clinical genetics"> clinical genetics</a>, <a href="https://publications.waset.org/abstracts/search?q=genotyping" title=" genotyping"> genotyping</a>, <a href="https://publications.waset.org/abstracts/search?q=oncology" title=" oncology"> oncology</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a> </p> <a href="https://publications.waset.org/abstracts/150896/using-multiomic-plasma-profiling-from-liquid-biopsies-to-identify-potential-signatures-for-disease-diagnostics-in-late-stage-non-small-cell-lung-cancer-nsclc-in-trinidad-and-tobago" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/150896.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">161</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">231</span> MicroRNA Profiling Reveals Novel Circulating Biomarkers in Acute Phase of Myocardial Infarction</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Maciejak">A. Maciejak</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Kiliszek"> M. Kiliszek</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Opolski"> G. Opolski</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Tulacz"> D. Tulacz</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Segiet"> A. Segiet</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Matlak"> K. Matlak</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Dobrzycki"> S. Dobrzycki</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Sygitowicz"> G. Sygitowicz</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Burzynska"> B. Burzynska</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Gora"> M. Gora</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction and aims: Acute myocardial infarction (AMI) is one of the most severe cardiovascular diseases affecting millions of patients each year worldwide. An early and accurate diagnosis of AMI is essential for optimal treatment. Therefore, new approaches that can complement and improve current strategies for AMI diagnosis are urgently needed. Recent studies have revealed the presence of stable circulating myocardial-derived microRNAs (miRNAs) in human peripheral blood, suggesting that such miRNAs could serve as potential biomarkers of infarction. The present study aimed to identify differentially expressed circulating miRNAs in ST-segment elevation myocardial infarction (STEMI) patients. Materials and methods: miRNA expression profile analysis was performed using Exiqon Serum/Plasma Focus microRNA PCR panel in plasma samples of n=16 patients on the first day of AMI (admission) and in samples from the same patients collected six months after AMI. Selected miRNAs were validated by RT-qPCR using serum samples from an independent set of n=14 AMI patients. Results: The profiling study identified 46 species of plasma miRNAs that were differentially expressed (p < 0.05) on admission compared to six months after AMI. The validation in the independent group of patients confirmed that miR-133b and miR-22-5p were significantly up-regulated upon AMI. Conclusions: Our results suggest that miRNA expression profiling provides better understanding of the changes that occur in the acute phase of MI in the myocardium and could be useful in determination of the potential role of extracellular miRNAs as paracrine signaling molecules. miR-22-5p represents a novel promising biomarker for the diagnosis of acute myocardial infarction. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acute%20myocardial%20infarction" title="acute myocardial infarction">acute myocardial infarction</a>, <a href="https://publications.waset.org/abstracts/search?q=circulating%20microRNAs" title=" circulating microRNAs"> circulating microRNAs</a>, <a href="https://publications.waset.org/abstracts/search?q=microRNA%20expression%20profiling" title=" microRNA expression profiling"> microRNA expression profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=miR-22-5p" title=" miR-22-5p"> miR-22-5p</a> </p> <a href="https://publications.waset.org/abstracts/40104/microrna-profiling-reveals-novel-circulating-biomarkers-in-acute-phase-of-myocardial-infarction" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40104.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">330</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">230</span> Metabolic Profiling in Breast Cancer Applying Micro-Sampling of Biological Fluids and Analysis by Gas Chromatography – Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M%C3%B3nica%20P.%20Cala">Mónica P. Cala</a>, <a href="https://publications.waset.org/abstracts/search?q=Juan%20S.%20Carre%C3%B1o"> Juan S. Carreño</a>, <a href="https://publications.waset.org/abstracts/search?q=Roland%20J.W.%20Meesters"> Roland J.W. Meesters</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recently, collection of biological fluids on special filter papers has become a popular micro-sampling technique. Especially, the dried blood spot (DBS) micro-sampling technique has gained much attention and is momently applied in various life sciences reserach areas. As a result of this popularity, DBS are not only intensively competing with the venous blood sampling method but are at this moment widely applied in numerous bioanalytical assays. In particular, in the screening of inherited metabolic diseases, pharmacokinetic modeling and in therapeutic drug monitoring. Recently, microsampling techniques were also introduced in “omics” areas, whereunder metabolomics. For a metabolic profiling study we applied micro-sampling of biological fluids (blood and plasma) from healthy controls and from women with breast cancer. From blood samples, dried blood and plasma samples were prepared by spotting 8uL sample onto pre-cutted 5-mm paper disks followed by drying of the disks for 100 minutes. Dried disks were then extracted by 100 uL of methanol. From liquid blood and plasma samples 40 uL were deproteinized with methanol followed by centrifugation and collection of supernatants. Supernatants and extracts were evaporated until dryness by nitrogen gas and residues derivated by O-methyxyamine and MSTFA. As internal standard C17:0-methylester in heptane (10 ppm) was used. Deconvolution and alignment of and full scan (m/z 50-500) MS data were done by AMDIS and SpectConnect (http://spectconnect.mit.edu) software, respectively. Statistical Data analysis was done by Principal Component Analysis (PCA) using R software. The results obtained from our preliminary study indicate that the use of dried blood/plasma on paper disks could be a powerful new tool in metabolic profiling. Many of the metabolites observed in plasma (liquid/dried) were also positively identified in whole blood samples (liquid/dried). Whole blood could be a potential substitute matrix for plasma in Metabolomic profiling studies as well also micro-sampling techniques for the collection of samples in clinical studies. It was concluded that the separation of the different sample methodologies (liquid vs. dried) as observed by PCA was due to different sample treatment protocols applied. More experiments need to be done to confirm obtained observations as well also a more rigorous validation .of these micro-sampling techniques is needed. The novelty of our approach can be found in the application of different biological fluid micro-sampling techniques for metabolic profiling. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofluids" title="biofluids">biofluids</a>, <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title=" breast cancer"> breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolic%20profiling" title=" metabolic profiling"> metabolic profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=micro-sampling" title=" micro-sampling"> micro-sampling</a> </p> <a href="https://publications.waset.org/abstracts/30894/metabolic-profiling-in-breast-cancer-applying-micro-sampling-of-biological-fluids-and-analysis-by-gas-chromatography-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30894.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">411</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">229</span> Strengths Profiling: An Alternative Approach to Assessing Character Strengths Based on Personal Construct Psychology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sam%20J.%20Cooley">Sam J. Cooley</a>, <a href="https://publications.waset.org/abstracts/search?q=Mary%20L.%20Quinton"> Mary L. Quinton</a>, <a href="https://publications.waset.org/abstracts/search?q=Benjamin%20J.%20Parry"> Benjamin J. Parry</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20J.%20G.%20Holland"> Mark J. G. Holland</a>, <a href="https://publications.waset.org/abstracts/search?q=Richard%20J.%20Whiting"> Richard J. Whiting</a>, <a href="https://publications.waset.org/abstracts/search?q=Jennifer%20Cumming"> Jennifer Cumming</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Practitioners draw attention to people’s character strengths to promote empowerment and well-being. This paper explores the possibility that existing approaches for assessing character strengths (e.g., the Values in Action survey; VIA-IS) could be even more autonomy supportive and empowering when combined with strengths profiling, an ideographic tool informed by personal construct theory (PCT). A PCT approach ensures that: (1) knowledge is co-created (i.e., the practitioner is not seen as the ‘expert’ who leads the process); (2) individuals are not required to ‘fit’ within a prescribed list of characteristics; and (3) individuals are free to use their own terminology and interpretations. A combined Strengths Profiling and VIA approach was used in a sample of homeless youth (aged 16-25) who are commonly perceived as ‘hard-to-engage’ through traditional forms of assessment. Strengths Profiling was completed face-to-face in small groups. Participants (N = 116) began by listing a variety of personally meaningful characteristics. Participants gave each characteristic a score out of ten for how important it was to them (1 = not so important; 10 = very important), their ideal competency, and their current competency (1 = poor; 10 = excellent). A discrepancy score was calculated for each characteristic (discrepancy score = ideal score - current score x importance), whereby a lower discrepancy score indicated greater satisfaction. Strengths Profiling was used at the beginning and end of a 10-week positive youth development programme. Experiences were captured through video diary room entries made by participants and through reflective notes taken by the facilitators. Participants were also asked to complete a pre-and post-programme questionnaire, measuring perceptions of well-being, self-worth, and resilience. All of the young people who attended the strengths profiling session agreed to complete a profile, and the majority became highly engaged in the process. Strengths profiling was found to be an autonomy supportive and empowering experience, with each participant identifying an average of 10 character strengths (M = 10.27, SD = 3.23). In total, 215 different character strengths were identified, each with varying terms and definitions used, which differed greatly between participants and demonstrated the value in soliciting personal constructs. Using the participants’ definitions, 98% of characteristics were categorized deductively into the VIA framework. Bravery, perseverance, and hope were the character strengths that featured most, whilst temperance and courage received the highest discrepancy scores. Discrepancy scores were negatively correlated with well-being, self-worth, and resilience, and meaningful improvements were recorded following the intervention. These findings support the use of strengths profiling as a theoretically-driven and novel way to engage disadvantaged youth in identifying and monitoring character strengths. When young people are given the freedom to express their own characteristics, the resulting terminologies extend beyond the language used in existing frameworks. This added freedom and control over the process of strengths identification encouraged youth to take ownership over their profiles and apply their strengths. In addition, the ability to transform characteristics post hoc into the VIA framework means that strengths profiling can be used to explore aggregated/nomothetic hypotheses, whilst still benefiting from its ideographic roots. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ideographic" title="ideographic">ideographic</a>, <a href="https://publications.waset.org/abstracts/search?q=nomothetic" title=" nomothetic"> nomothetic</a>, <a href="https://publications.waset.org/abstracts/search?q=positive%20youth%20development" title=" positive youth development"> positive youth development</a>, <a href="https://publications.waset.org/abstracts/search?q=VIA-IS" title=" VIA-IS"> VIA-IS</a>, <a href="https://publications.waset.org/abstracts/search?q=assessment" title=" assessment"> assessment</a>, <a href="https://publications.waset.org/abstracts/search?q=homeless%20youth" title=" homeless youth"> homeless youth</a> </p> <a href="https://publications.waset.org/abstracts/91297/strengths-profiling-an-alternative-approach-to-assessing-character-strengths-based-on-personal-construct-psychology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91297.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">201</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">228</span> Lipidomic Profiling of Chlorella sp. and Scenedesmus abundans towards Deciphering Phospholipids and Glycolipids under Nitrogen Limited Condition</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=J.%20Singh">J. Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Swati%20Dubey"> Swati Dubey</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20P.%20Singh"> R. P. Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microalgal strains can accumulate greatly enhanced levels of lipids under nitrogen-deficient condition, making these as one of the most promising sustainable sources for biofuel production. High-grade biofuel production from microalgal biomass could be facilitated by analysing the lipid content of the microalgae and enumerating its dynamics under varying nutrient conditions. In the present study, a detailed investigation of changes in lipid composition in Chlorella species and Scenedesmus abundans in response to nitrogen limited condition was performed to provide novel mechanistic insights into the lipidome during stress conditions. The mass spectroscopic approaches mainly LC-MS and GC-MS were employed for lipidomic profiling in both the microalgal strains. The analyses of lipid profiling using LC-MS revealed distinct forms of lipids mainly phospho- and glycolipids, including betaine lipids, and various other forms of lipids in both the microalgal strains. As detected, an overall decrease in polar lipids was observed. However, GC-MS analyses had revealed that the synthesis of the storage lipid i.e. triacylglycerol (TAG) was substantially stimulated in both the strains under nitrogen limited conditions. The changes observed in the overall fatty acid profile were primarily due to the decrease in proportion of polar lipids to TAGs. This study had enabled in analysing a detailed and orchestrated form of lipidomes in two different microalgal strains having potential for biodiesel production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofuel" title="biofuel">biofuel</a>, <a href="https://publications.waset.org/abstracts/search?q=GC-MS" title=" GC-MS"> GC-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=LC-MS" title=" LC-MS"> LC-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid" title=" lipid"> lipid</a>, <a href="https://publications.waset.org/abstracts/search?q=microalgae" title=" microalgae"> microalgae</a> </p> <a href="https://publications.waset.org/abstracts/74880/lipidomic-profiling-of-chlorella-sp-and-scenedesmus-abundans-towards-deciphering-phospholipids-and-glycolipids-under-nitrogen-limited-condition" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74880.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">370</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">227</span> Comprehensive Profiling and Characterization of Untargeted Extracellular Metabolites in Fermentation Processes: Insights and Advances in Analysis and Identification</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marianna%20Ciaccia">Marianna Ciaccia</a>, <a href="https://publications.waset.org/abstracts/search?q=Gennaro%20Agrimi"> Gennaro Agrimi</a>, <a href="https://publications.waset.org/abstracts/search?q=Isabella%20Pisano"> Isabella Pisano</a>, <a href="https://publications.waset.org/abstracts/search?q=Maurizio%20Bettiga"> Maurizio Bettiga</a>, <a href="https://publications.waset.org/abstracts/search?q=Silvia%20Rapacioli"> Silvia Rapacioli</a>, <a href="https://publications.waset.org/abstracts/search?q=Giulia%20Mensa"> Giulia Mensa</a>, <a href="https://publications.waset.org/abstracts/search?q=Monica%20Marzagalli"> Monica Marzagalli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: Untargeted metabolomic analysis of extracellular metabolites is a powerful approach that focuses on comprehensively profiling in the extracellular space. In this study, we applied extracellular metabolomic analysis to investigate the metabolism of two probiotic microorganisms with health benefits that extend far beyond the digestive tract and the immune system. Methods: Analytical techniques employed in extracellular metabolomic analysis encompass various technologies, including mass spectrometry (MS), which enables the identification of metabolites present in the fermentation media, as well as the comparison of metabolic profiles under different experimental conditions. Multivariate statistical analysis techniques like principal component analysis (PCA) or partial least squares-discriminant analysis (PLS-DA) play a crucial role in uncovering metabolic signatures and understanding the dynamics of metabolic networks. Results: Different types of supernatants from fermentation processes, such as dairy-free, not dairy-free media and media with no cells or pasteurized, were subjected to metabolite profiling, which contained a complex mixture of metabolites, including substrates, intermediates, and end-products. This profiling provided insights into the metabolic activity of the microorganisms. The integration of advanced software tools has facilitated the identification and characterization of metabolites in different fermentation conditions and microorganism strains. Conclusions: In conclusion, untargeted extracellular metabolomic analysis, combined with software tools, allowed the study of the metabolites consumed and produced during the fermentation processes of probiotic microorganisms. Ongoing advancements in data analysis methods will further enhance the application of extracellular metabolomic analysis in fermentation research, leading to improved bioproduction and the advancement of sustainable manufacturing processes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biotechnology" title="biotechnology">biotechnology</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolomics" title=" metabolomics"> metabolomics</a>, <a href="https://publications.waset.org/abstracts/search?q=lactic%20bacteria" title=" lactic bacteria"> lactic bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=probiotics" title=" probiotics"> probiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=postbiotics" title=" postbiotics"> postbiotics</a> </p> <a href="https://publications.waset.org/abstracts/181567/comprehensive-profiling-and-characterization-of-untargeted-extracellular-metabolites-in-fermentation-processes-insights-and-advances-in-analysis-and-identification" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/181567.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">71</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">226</span> Use of Pig as an Animal Model for Assessing the Differential MicroRNA Profiling in Kidney after Aristolochic Acid Intoxication</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Daniela%20E.%20Marin">Daniela E. Marin</a>, <a href="https://publications.waset.org/abstracts/search?q=Cornelia%20Braicu"> Cornelia Braicu</a>, <a href="https://publications.waset.org/abstracts/search?q=Gina%20C.%20Pistol"> Gina C. Pistol</a>, <a href="https://publications.waset.org/abstracts/search?q=Roxana%20Cojocneanu-Petric"> Roxana Cojocneanu-Petric</a>, <a href="https://publications.waset.org/abstracts/search?q=Ioana%20Berindan%20Neagoe"> Ioana Berindan Neagoe</a>, <a href="https://publications.waset.org/abstracts/search?q=Mihail%20A.%20Gras"> Mihail A. Gras</a>, <a href="https://publications.waset.org/abstracts/search?q=Ionelia%20Taranu"> Ionelia Taranu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aristolochic acid (AA) is a carcinogenic, mutagenic, and nephrotoxic compound commonly found in the Aristolochiaceae family of plants. AA is frequently associated with urothelial carcinoma of the upper urinary tract in human and animals and is considered as being responsible for Balkan Endemic Nephropathy. The pig provides a good animal model because the porcine urological system is very similar to that of humans, both in aspects of physiology and anatomy. MicroRNA (miRNA) are small non-coding RNAs that have an impact on a wide range of biological processes by regulating gene expression at post-transcriptional level. The objective of this study was to analyze the miRNA profiling in the kidneys of AA intoxicated swine. For this purpose, ten TOPIGS-40 crossbred weaned piglets, 4-week-old, male and females with an initial average body weight of 9.83 ± 0.5 kg were studied for 28 days. They were given ad libitum access to water and feed and randomly allotted to one of the following groups: control group (C) or aristolochic acid group (AA). They were fed a maize-soybean-meal-based diet contaminated or not with 0.25mgAA/kg. To profile miRNA in the kidneys of pigs, microarrays and bioinformatics approaches were applied to analyze the miRNA in the kidney of control and AA intoxicated pigs. After normalization, our results have shown that a total of 5 known miRNAs and 4 novel miRNAs had different profiling in the kidney of intoxicated animals versus control ones. Expression of miR-32-5p, miR-497-5p, miR-423-3p, miR-218-5p, miR-128-3p were up-regulated by 0.25mgAA/kg feed, while the expression of miR-9793-5p, miR-9835-3p, miR-9840-3p, miR-4334-5p was down-regulated. The microRNA profiling in kidney of intoxicated animals was associated with modified expression of target genes as: RICTOR, LASP1, SFRP2, DKK2, BMI1, RAF1, IGF1R, MAP2K1, WEE1, HDGF, BCL2, EIF4E etc, involved in cell division cycle, apoptosis, cell differentiation and cell migration, cell signaling, cancer etc. In conclusion, this study provides new data concerning the microRNA profiling in kidney after aristolochic acid intoxications with important implications for human and animal health. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aristolochic%20acid" title="aristolochic acid">aristolochic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=kidney" title=" kidney"> kidney</a>, <a href="https://publications.waset.org/abstracts/search?q=microRNA" title=" microRNA"> microRNA</a>, <a href="https://publications.waset.org/abstracts/search?q=swine" title=" swine"> swine</a> </p> <a href="https://publications.waset.org/abstracts/67527/use-of-pig-as-an-animal-model-for-assessing-the-differential-microrna-profiling-in-kidney-after-aristolochic-acid-intoxication" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/67527.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">283</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">225</span> Exploring the Profiles of Militants in the SWAT Valley of Pakistan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lateef%20Hakim%20Zai%20Khyber">Lateef Hakim Zai Khyber</a>, <a href="https://publications.waset.org/abstracts/search?q=Syed%20Rashid%20Ali"> Syed Rashid Ali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the post 9/11 era, a new trend has developed of terrorist profiling on the basis of the ethnic, religious, political, psychological, social, and economic background of the terrorists to anticipate and assess the possible risk and to prevent and prosecute the suspected before they commit any violent act. The same profiling approach was adopted in different militant or terrorist de-radicalization and rehabilitation programs across the world in order to evaluate and identify the reasons and causes for joining terrorism in terms of push and pull factors. This paper attempts to explore and investigate the profiles of the detainees in the Sabaoon de-radicalization and Emancipation program, which aimed at de-radicalizing the former militants of Tehrik-e-Taliban (TTP) Pakistan in the Swat valley of Pakistan. This research attempted to use qualitative methods for collecting data, including a number of formal and informal open-ended interviews with the former staff members of Sabaoon to explore various aspects of the program, such as various approaches used at Sabaoon for terrorist profiling. It conducts a thorough examination of the profiles of the terrorist through their socioeconomic, ideological, emotional, intellectual, and psychological conditions and orientations, personal details, family issues, social preferences, etc. The study finds out that the majority of the terrorists belonged to the marginalized groups or lower class, including underprivileged tenants and poor laborers, of society having no access to land. They possess almost the same profiles, including low socioeconomic status, absence of a father or strict behavior of parents, large and combined families, lack of education, lack of religious understanding, etc. They also possess some common traits such as anxiety disorder, emotional instability, aggressive impulses and insecurity, depression, inferiority complex, lack of critical thinking and logical reasoning, authority-seeking behavior, and revenge-seeking behavior. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=terrorist%20profiling" title="terrorist profiling">terrorist profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=Sabaoon" title=" Sabaoon"> Sabaoon</a>, <a href="https://publications.waset.org/abstracts/search?q=de-radicalization" title=" de-radicalization"> de-radicalization</a>, <a href="https://publications.waset.org/abstracts/search?q=rehabilitation" title=" rehabilitation"> rehabilitation</a>, <a href="https://publications.waset.org/abstracts/search?q=Swat" title=" Swat"> Swat</a>, <a href="https://publications.waset.org/abstracts/search?q=Pakistan" title=" Pakistan"> Pakistan</a>, <a href="https://publications.waset.org/abstracts/search?q=juvenile%20militants" title=" juvenile militants"> juvenile militants</a> </p> <a href="https://publications.waset.org/abstracts/142976/exploring-the-profiles-of-militants-in-the-swat-valley-of-pakistan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/142976.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">155</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">224</span> Business Intelligence for Profiling of Telecommunication Customer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rokhmatul%20Insani">Rokhmatul Insani</a>, <a href="https://publications.waset.org/abstracts/search?q=Hira%20Laksmiwati%20Soemitro"> Hira Laksmiwati Soemitro</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Business Intelligence is a methodology that exploits the data to produce information and knowledge systematically, business intelligence can support the decision-making process. Some methods in business intelligence are data warehouse and data mining. A data warehouse can store historical data from transactional data. For data modelling in data warehouse, we apply dimensional modelling by Kimball. While data mining is used to extracting patterns from the data and get insight from the data. Data mining has many techniques, one of which is segmentation. For profiling of telecommunication customer, we use customer segmentation according to customer’s usage of services, customer invoice and customer payment. Customers can be grouped according to their characteristics and can be identified the profitable customers. We apply K-Means Clustering Algorithm for segmentation. The input variable for that algorithm we use RFM (Recency, Frequency and Monetary) model. All process in data mining, we use tools IBM SPSS modeller. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=business%20intelligence" title="business intelligence">business intelligence</a>, <a href="https://publications.waset.org/abstracts/search?q=customer%20segmentation" title=" customer segmentation"> customer segmentation</a>, <a href="https://publications.waset.org/abstracts/search?q=data%20warehouse" title=" data warehouse"> data warehouse</a>, <a href="https://publications.waset.org/abstracts/search?q=data%20mining" title=" data mining"> data mining</a> </p> <a href="https://publications.waset.org/abstracts/46969/business-intelligence-for-profiling-of-telecommunication-customer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46969.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">484</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">223</span> HPTLC Metabolite Fingerprinting of Artocarpus champeden Stembark from Several Different Locations in Indonesia and Correlation with Antimalarial Activity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Imam%20Taufik">Imam Taufik</a>, <a href="https://publications.waset.org/abstracts/search?q=Hilkatul%20Ilmi"> Hilkatul Ilmi</a>, <a href="https://publications.waset.org/abstracts/search?q=Puryani"> Puryani</a>, <a href="https://publications.waset.org/abstracts/search?q=Mochammad%20Yuwono"> Mochammad Yuwono</a>, <a href="https://publications.waset.org/abstracts/search?q=Aty%20Widyawaruyanti"> Aty Widyawaruyanti</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Artocarpus champeden Spreng stembark (Moraceae) in Indonesia well known as ‘cempedak’ had been traditionally used for malarial remedies. The difference of growth locations could cause the difference of metabolite profiling. As a consequence, there were difference antimalarial activities in spite of the same plants. The aim of this research was to obtain the profile of metabolites that contained in A. champeden stembark from different locations in Indonesia for authentication and quality control purpose of this extract. The profiling had been performed by HPTLC-Densitometry technique and antimalarial activity had been also determined by HRP2-ELISA technique. The correlation between metabolite fingerprinting and antimalarial activity had been analyzed by Principle Component Analysis, Hierarchical Clustering Analysis and Partial Least Square. As a result, there is correlation between the difference metabolite fingerprinting and antimalarial activity from several different growth locations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antimalarial" title="antimalarial">antimalarial</a>, <a href="https://publications.waset.org/abstracts/search?q=artocarpus%20champeden%20spreng" title=" artocarpus champeden spreng"> artocarpus champeden spreng</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolite%20fingerprinting" title=" metabolite fingerprinting"> metabolite fingerprinting</a>, <a href="https://publications.waset.org/abstracts/search?q=multivariate%20analysis" title=" multivariate analysis"> multivariate analysis</a> </p> <a href="https://publications.waset.org/abstracts/58321/hptlc-metabolite-fingerprinting-of-artocarpus-champeden-stembark-from-several-different-locations-in-indonesia-and-correlation-with-antimalarial-activity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58321.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">311</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">222</span> Gut Metabolite Profiling of the Ethnic Groups from Assam, India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Madhusmita%20Dehingia">Madhusmita Dehingia</a>, <a href="https://publications.waset.org/abstracts/search?q=Supriyo%20Sen"> Supriyo Sen</a>, <a href="https://publications.waset.org/abstracts/search?q=Bhuwan%20Bhaskar"> Bhuwan Bhaskar</a>, <a href="https://publications.waset.org/abstracts/search?q=Tulsi%20Joishy"> Tulsi Joishy</a>, <a href="https://publications.waset.org/abstracts/search?q=Mojibur%20R.%20Khan"> Mojibur R. Khan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Human gut microbes and their metabolites are important for maintaining homeostasis in the gut and are responsible for many metabolic and immune mediated diseases. In the present study, we determined the profiles of the gut metabolites of five different ethnic groups (Bodo, Tai-Phake, Karbi, Tea tribe and Tai-Aiton) of Assam. Fecal metabolite profiling of the 39 individuals belonging to the ethnic groups was carried out using Gas chromatography – Mass spectrometry (GC-MS), and comparison was performed among the tribes for common and unique metabolites produced within their gut. Partial Least Squares Discriminant Analysis (PLS-DA) of the metabolites suggested that the individuals grouped according to their ethnicity. Among the 66 abundant metabolites, 12 metabolites were found to be common among the five ethnic groups. Additionally, ethnicity wise some unique metabolites were also detected. For example, the tea tribe of Assam contained the tea components, Aniline and Benzoate more in their gut in comparison to others. Metabolites of microbial origin were also correlated with the already published metagenomic data of the same ethnic group and functional analysis were carried out based on human metabolome database. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ethnicity" title="ethnicity">ethnicity</a>, <a href="https://publications.waset.org/abstracts/search?q=gut%20microbiota" title=" gut microbiota"> gut microbiota</a>, <a href="https://publications.waset.org/abstracts/search?q=GC-MS" title=" GC-MS"> GC-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolites" title=" metabolites"> metabolites</a> </p> <a href="https://publications.waset.org/abstracts/60440/gut-metabolite-profiling-of-the-ethnic-groups-from-assam-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/60440.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">422</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">221</span> Approach to Honey Volatiles' Profiling by Gas Chromatography and Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Igor%20Jerkovic">Igor Jerkovic</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Biodiversity of flora provides many different nectar sources for the bees. Unifloral honeys possess distinctive flavours, mainly derived from their nectar sources (characteristic volatile organic components (VOCs)). Specific or nonspecific VOCs (chemical markers) could be used for unifloral honey characterisation as addition to the melissopalynologycal analysis. The main honey volatiles belong, in general, to three principal categories: terpenes, norisoprenoids, and benzene derivatives. Some of these substances have been described as characteristics of the floral source, and other compounds, like several alcohols, branched aldehydes, and furan derivatives, may be related to the microbial purity of honey processing and storage conditions. Selection of the extraction method for the honey volatiles profiling should consider that heating of the honey produce different artefacts and therefore conventional methods of VOCs isolation (such as hydrodistillation) cannot be applied for the honey. Two-way approach for the isolation of the honey VOCs was applied using headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE). The extracts were analysed by gas chromatography and mass spectrometry (GC-MS). HS-SPME (with the fibers of different polarity such as polydimethylsiloxane/ divinylbenzene (PDMS/DVB) or divinylbenzene/carboxene/ polydimethylsiloxane (DVB/CAR/PDMS)) enabled isolation of high volatile headspace VOCs of the honey samples. Among them, some characteristic or specific compounds can be found such as 3,4-dihydro-3-oxoedulan (in Centaurea cyanus L. honey) or 1H-indole, methyl anthranilate, and cis-jasmone (in Citrus unshiu Marc. honey). USE with different solvents (mainly dichloromethane or the mixture pentane : diethyl ether 1 : 2 v/v) enabled isolation of less volatile and semi-volatile VOCs of the honey samples. Characteristic compounds from C. unshiu honey extracts were caffeine, 1H-indole, 1,3-dihydro-2H-indol-2-one, methyl anthranilate, and phenylacetonitrile. Sometimes, the selection of solvent sequence was useful for more complete profiling such as sequence I: pentane → diethyl ether or sequence II: pentane → pentane/diethyl ether (1:2, v/v) → dichloromethane). The extracts with diethyl ether contained hydroquinone and 4-hydroxybenzoic acid as the major compounds, while (E)-4-(r-1’,t-2’,c-4’-trihydroxy-2’,6’,6’-trimethylcyclo-hexyl)but-3-en-2-one predominated in dichloromethane extracts of Allium ursinum L. honey. With this two-way approach, it was possible to obtain a more detailed insight into the honey volatile and semi-volatile compounds and to minimize the risks of compound discrimination due to their partial extraction that is of significant importance for the complete honey profiling and identification of the chemical biomarkers that can complement the pollen analysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=honey%20chemical%20biomarkers" title="honey chemical biomarkers">honey chemical biomarkers</a>, <a href="https://publications.waset.org/abstracts/search?q=honey%20volatile%20compounds%20profiling" title=" honey volatile compounds profiling"> honey volatile compounds profiling</a>, <a href="https://publications.waset.org/abstracts/search?q=headspace%20solid-phase%20microextraction%20%28HS-SPME%29" title=" headspace solid-phase microextraction (HS-SPME)"> headspace solid-phase microextraction (HS-SPME)</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasonic%20solvent%20extraction%20%28USE%29" title=" ultrasonic solvent extraction (USE)"> ultrasonic solvent extraction (USE)</a> </p> <a href="https://publications.waset.org/abstracts/81756/approach-to-honey-volatiles-profiling-by-gas-chromatography-and-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/81756.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">202</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=6">6</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=7">7</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=8">8</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=9">9</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=profiling&page=2" rel="next">&rsaquo;</a></li> 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