Springer Protocols platform has migrated to Experiments

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coli" name="organismFacet" id="organism-facet_Escherichia_coli" type="checkbox" autocomplete="off" tabindex="0"> <label class="facet-value-title" for="organism-facet_Escherichia_coli">Escherichia coli</label> <span class="facet-value-count">6957</span> <div class="control__indicator" tabindex="0"></div> </li> <li class="facet-value at-4 control"> <input class="facet-checkbox" value="Bos taurus" name="organismFacet" id="organism-facet_Bos_taurus" type="checkbox" autocomplete="off" tabindex="0"> <label class="facet-value-title" for="organism-facet_Bos_taurus">Bos taurus</label> <span class="facet-value-count">3834</span> <div class="control__indicator" tabindex="0"></div> </li> <li class="facet-value at-5 control"> <input class="facet-checkbox" value="Rattus norvegicus" name="organismFacet" id="organism-facet_Rattus_norvegicus" type="checkbox" autocomplete="off" tabindex="0"> <label class="facet-value-title" for="organism-facet_Rattus_norvegicus">Rattus norvegicus</label> <span 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class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Molecular Biology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7651_2015_299, Springer Protocols, 1' data-track-category='mosaic:search-page' href='/articles/10.1007/7651_2015_299'>Effects of Malignant Melanoma Initiating Cells on T-Cell Activation</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Tobias Schatton </span> <span class="comma_space">, </span> <span class="author-name">Ute Schütte </span> <span class="comma_space">, </span> <span class="author-name">Markus H. Frank <a class="author-email" href="mailto:markus.frank@childrens.harvard.edu"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p>Although human malignant melanoma is a highly immunogenic cancer, both the endogenous antitumor immune response and melanoma immunotherapy often fail to control neoplastic progression. Accordingly, characterizing melanoma cell subsets capable of</p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7651_2015_299'>more</a> </span> </div> <div class="snippet-details hidden"><p>Although human malignant melanoma is a highly immunogenic cancer, both the endogenous antitumor immune response and melanoma immunotherapy often fail to control neoplastic progression. Accordingly, characterizing melanoma cell subsets capable of evading antitumor immunity could unravel optimized treatment strategies that might reduce morbidity and mortality from melanoma. By virtue of their preferential capacity to modulate antitumor immune responses and drive inexorable tumor growth and progression, malignant melanoma-initiating cells (MMICs) warrant closer investigation to further elucidate the cellular and molecular mechanisms underlying melanoma immune evasion and immunotherapy resistance. Here we describe methodologies that enable the characterization of immunoregulatory effects of purified MMICs versus melanoma bulk populations in coculture with syngeneic or allogeneic lymphocytes, using [<sup>3</sup>H]thymidine incorporation, enzyme-linked immunosorbent spot (ELISPOT), or ELISA assays. These assays were traditionally developed to analyze alloimmune processes and we successfully adapted them for the study of tumor-mediated immunomodulatory functions.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p class="collapsed"> <span>Techniques: </span>Immunomodulation, ELISA, Enzyme-linked Immunospot Assay (ELISPOT), Co-culture, Cell And Tissue Culture... <a class="more-link" href="#">2 more</a> </p> <p class="expanded hidden"> <span>Techniques: </span>Immunomodulation, ELISA, Enzyme-linked Immunospot Assay (ELISPOT), Co-culture, Cell And Tissue Culture, Cell Proliferation Assay, Density-gradient Centrifugation <a class="less-link" href="#">less</a> </p> </div> </div> <div class="snippet-techniques"> <div class="models"> <p><span>Models: </span>Homo sapiens</p> </div> </div> <div class="snippet-techniques"> <div class="antibodies"> <p><span>Antibodies: </span>Anti-mouse, Anti-human</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="views"> Downloads: <span class="green-text">89</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7651_2015_299</div> <div class="image-search hiddenDesktop"> </div> <div class="image-search hiddenMobile"> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Molecular Biology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7651_2020_299, Springer Protocols, 2' data-track-category='mosaic:search-page' href='/articles/10.1007/7651_2020_299'>The Characteristics of Human iPS Cells and siRNA Transfection Under Hypoxia</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Kouji Sugimoto </span> <span class="comma_space">, </span> <span class="author-name">Yoshihiko Hayashi <a class="author-email" href="mailto:hayashi@nagasaki-u.ac.jp"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p>The characteristics of pluripotent cells have great potential for basic and clinical research and application. We describe the effect of normoxia or hypoxia regarding the proliferation and pluripotency of human iPS cells using colony number counting </p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7651_2020_299'>more</a> </span> </div> <div class="snippet-details hidden"><p>The characteristics of pluripotent cells have great potential for basic and clinical research and application. We describe the effect of normoxia or hypoxia regarding the proliferation and pluripotency of human iPS cells using colony number counting and real-time polymerase chain reaction (PCR). In addition, the function of hypoxia-inducible factors (HIFs) in human iPS cells under hypoxic conditions is evaluated in relation to the expression of pluripotency markers by siRNA and real-time PCR. Furthermore, we introduce the change of HIF-2α expression when signal transducer and activator of transcription 3 (STAT3) is suppressed by its inhibitor, Stattic or S31 201, using RT-PCR.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p><span>Techniques: </span>Transfection, Cell And Tissue Culture, Real-time PCR, Reverse Transcription PCR, Co-culture</p> </div> </div> <div class="snippet-techniques"> <div class="models"> <p><span>Models: </span>Homo sapiens, Mus musculus</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="views"> Downloads: <span class="green-text">4,006</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7651_2020_299</div> <div class="image-search hiddenDesktop"> <a href="/articles/10.1007/7651_2020_299" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2020_299" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2020_299/MediaObjects/7651_2020_299_Fig1_HTML.png" alt="The number of human iPS cells under 5 or 20% oxygen on days 5 and 14 (*P < 0.05, ***P < 0.005) (reproduced from [17] with permission from Wiley)"> </a> <a href="/articles/10.1007/7651_2020_299" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2020_299" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2020_299/MediaObjects/7651_2020_299_Fig2_HTML.png" alt="The knockdown efficiency of HIFs in human iPS cells 7 days after knockdown (date from duplicate samples."> </a> <a href="/articles/10.1007/7651_2020_299" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2020_299" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2020_299/MediaObjects/7651_2020_299_Fig3_HTML.png" alt="The pluripotency marker mRNA expression under knockdown of HIFs on 7 days (*P < 0.05, **P < 0.01)."> </a> <a href="/articles/10.1007/7651_2020_299" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2020_299" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2020_299/MediaObjects/7651_2020_299_Fig4_HTML.png" alt="STAT3 mRNA expression 3 days after inhibition of STAT3 in human iPS cells under 5% oxygen (data from triplicate samples."> </a> </div> <div class="image-search hiddenMobile"> <a href="/articles/10.1007/7651_2020_299" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2020_299" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2020_299/MediaObjects/7651_2020_299_Fig1_HTML.png" alt="The number of human iPS cells under 5 or 20% oxygen on days 5 and 14 (*P < 0.05, ***P < 0.005) (reproduced from [17] with permission from Wiley)"> </a> <a href="/articles/10.1007/7651_2020_299" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2020_299" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2020_299/MediaObjects/7651_2020_299_Fig2_HTML.png" alt="The knockdown efficiency of HIFs in human iPS cells 7 days after knockdown (date from duplicate samples."> </a> <a href="/articles/10.1007/7651_2020_299" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2020_299" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2020_299/MediaObjects/7651_2020_299_Fig3_HTML.png" alt="The pluripotency marker mRNA expression under knockdown of HIFs on 7 days (*P < 0.05, **P < 0.01)."> </a> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Overview</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Pharmacology and Toxicology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7653_2020_47, Springer Protocols, 3' data-track-category='mosaic:search-page' href='/articles/10.1007/7653_2020_47'>History and Recent Advances in Coronavirus Discovery</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Sora Abdul-Fattah </span> <span class="comma_space">, </span> <span class="author-name">Aman Pal </span> <span class="comma_space">, </span> <span class="author-name">Nagham Kaka </span> <span class="comma_space">, </span> <span class="author-name">Pramath Kakodkar <a class="author-email" href="mailto:P.Kakodkar1@nuigalway.ie"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p>As of 6th September 2020, the COVID-19 pandemic has affected a total of 188 countries and has spread across to 26,924,131 people. The causal organism responsible for COVID-19 is SARS-CoV-2 which resurged from the coronavirus family. COVID-19 range</p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7653_2020_47'>more</a> </span> </div> <div class="snippet-details hidden"><p>As of 6th September 2020, the COVID-19 pandemic has affected a total of 188 countries and has spread across to 26,924,131 people. The causal organism responsible for COVID-19 is SARS-CoV-2 which resurged from the coronavirus family. COVID-19 range and rapidity of spread imposed a heavy burden on the healthcare services and led to stringent public help measures. The consequence of this is a detriment to the global economy in a background of continued morbidity and mortality from complications of COVID-19. Hence research and development dedicated to studying the coronavirus family may assist with discovering a COVID-19 specific treatment. This chapter aims to clarify the basics of coronavirus virology, i.e. taxonomy, virion structure and molecular pathophysiology. Furthermore, the history and origins of the human coronavirus cluster (HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1), as well as severe epidemic/pandemic causing coronaviruses (SARS-CoV-1, SARS-CoV-2 and MERS-CoV) is highlighted. As the coronavirus research is rapidly expanding, we have emphasized on a select few novel studies on the association of human coronaviruses with angiotensin-converting enzyme 2 receptor tissue distribution, Kawasaki Disease, Renin-Angiotensin-Aldosterone-System, repurposing antiviral agents, CoV main protease (M<sup>Pro</sup>) inhibitor, dexamethasone efficacy, BinaxNOW COVID-19 Ag Card and ABO blood group system.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="models"> <p><span>Models: </span>Homo sapiens</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="citation"> Citations: <span class="green-text">4</span> </span> <span class="separator"> | </span> <span class="views"> Downloads: <span class="green-text">1,167</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7653_2020_47</div> <div class="image-search hiddenDesktop"> <a href="/articles/10.1007/7653_2020_47" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2020_47" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2020_47/MediaObjects/7653_2020_47_Fig1_HTML.png" alt="Taxonomy of Nidovirales."> </a> <a href="/articles/10.1007/7653_2020_47" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2020_47" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2020_47/MediaObjects/7653_2020_47_Fig2_HTML.png" alt="Timeline of key developments in the field of coronavirus research and discoveries of novel human coronaviruses represented as blue and red boxes, respectively"> </a> <a href="/articles/10.1007/7653_2020_47" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2020_47" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2020_47/MediaObjects/7653_2020_47_Fig3_HTML.png" alt="Common structural components of all coronaviruses shown as a cross-section."> </a> <a href="/articles/10.1007/7653_2020_47" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2020_47" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2020_47/MediaObjects/7653_2020_47_Fig4_HTML.png" alt="The above illustrates the degree of each of ACE 2 and TMPRSS2 expression in different cell types found within the lung, oesophageal, ileum and colonic tissue."> </a> </div> <div class="image-search hiddenMobile"> <a href="/articles/10.1007/7653_2020_47" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2020_47" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2020_47/MediaObjects/7653_2020_47_Fig1_HTML.png" alt="Taxonomy of Nidovirales."> </a> <a href="/articles/10.1007/7653_2020_47" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2020_47" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2020_47/MediaObjects/7653_2020_47_Fig2_HTML.png" alt="Timeline of key developments in the field of coronavirus research and discoveries of novel human coronaviruses represented as blue and red boxes, respectively"> </a> <a href="/articles/10.1007/7653_2020_47" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2020_47" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2020_47/MediaObjects/7653_2020_47_Fig3_HTML.png" alt="Common structural components of all coronaviruses shown as a cross-section."> </a> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Molecular Biology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7651_2023_499, Springer Protocols, 4' data-track-category='mosaic:search-page' href='/articles/10.1007/7651_2023_499'>Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Pooja Flora </span> <span class="comma_space">, </span> <span class="author-name">Elena Ezhkova <a class="author-email" href="mailto:elena.ezhkova@mssm.edu"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p><span class="EmphasisTypeItalic">C</span>leavage <span class="EmphasisTypeItalic">U</span>nder <span class="EmphasisTypeItalic">T</span>argets & <span class="EmphasisTypeItalic">R</span>elease <span class="EmphasisTypeItalic">U</span>sing <span class="EmphasisTypeItalic">N</span>uclease (CUT&RUN) has swiftly become the preferred procedure over the past few years for genomic mapping and detecting interactions between chromatin and its bound proteins. CUT&RUN is now being wid</p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7651_2023_499'>more</a> </span> </div> <div class="snippet-details hidden"><p><span class="EmphasisTypeItalic">C</span>leavage <span class="EmphasisTypeItalic">U</span>nder <span class="EmphasisTypeItalic">T</span>argets & <span class="EmphasisTypeItalic">R</span>elease <span class="EmphasisTypeItalic">U</span>sing <span class="EmphasisTypeItalic">N</span>uclease (CUT&RUN) has swiftly become the preferred procedure over the past few years for genomic mapping and detecting interactions between chromatin and its bound proteins. CUT&RUN is now being widely used for characterizing the epigenetic landscape in many cell types as it utilizes far less cell numbers when compared to <span class="EmphasisTypeItalic">Ch</span>romatin <span class="EmphasisTypeItalic">I</span>mmuno<span class="EmphasisTypeItalic">p</span>recipitation-sequencing (ChIP-seq), thereby making it a powerful tool for researchers working with limited material. This protocol has been specifically optimized for detecting histone modifications in fluorescence-activated cell sorting (FACS)-isolated epidermal stem cells from adult mice.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p class="collapsed"> <span>Techniques: </span>Cell Separation And Isolation, Library Preparation, Data analysis, FACS, Cell And Tissue Culture... <a class="more-link" href="#">7 more</a> </p> <p class="expanded hidden"> <span>Techniques: </span>Cell Separation And Isolation, Library Preparation, Data analysis, FACS, Cell And Tissue Culture, PCR, DNA Purification, DNA Ligation, Bowtie, Genomics, Genome Visualization, ChIP-seq <a class="less-link" href="#">less</a> </p> </div> </div> <div class="snippet-techniques"> <div class="antibodies"> <p><span>Antibodies: </span>Anti-IgG, Anti-Histone, Anti-rabbit</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="citation"> Citations: <span class="green-text">1</span> </span> <span class="separator"> | </span> <span class="views"> Downloads: <span class="green-text">675</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7651_2023_499</div> <div class="image-search hiddenDesktop"> <a href="/articles/10.1007/7651_2023_499" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2023_499" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2023_499/MediaObjects/7651_2023_499_Fig1_HTML.png" alt="Isolation of EpSCs from adult murine skin."> </a> <a href="/articles/10.1007/7651_2023_499" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2023_499" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2023_499/MediaObjects/7651_2023_499_Fig2_HTML.png" alt="Quality assessment of CUT&RUN libraries."> </a> <a href="/articles/10.1007/7651_2023_499" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2023_499" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2023_499/MediaObjects/7651_2023_499_Fig3_HTML.png" alt="Integrated Genome Viewer (IGV) tracks of H3K27me3."> </a> </div> <div class="image-search hiddenMobile"> <a href="/articles/10.1007/7651_2023_499" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2023_499" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2023_499/MediaObjects/7651_2023_499_Fig1_HTML.png" alt="Isolation of EpSCs from adult murine skin."> </a> <a href="/articles/10.1007/7651_2023_499" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2023_499" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2023_499/MediaObjects/7651_2023_499_Fig2_HTML.png" alt="Quality assessment of CUT&RUN libraries."> </a> <a href="/articles/10.1007/7651_2023_499" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2023_499" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2023_499/MediaObjects/7651_2023_499_Fig3_HTML.png" alt="Integrated Genome Viewer (IGV) tracks of H3K27me3."> </a> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Molecular Biology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7651_2020_313, Springer Protocols, 5' data-track-category='mosaic:search-page' href='/articles/10.1007/7651_2020_313'>Selective Regional Isolation of Brain Microvessels</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Fernanda Medina-Flores </span> <span class="comma_space">, </span> <span class="author-name">Gabriela Hurtado-Alvarado </span> <span class="comma_space">, </span> <span class="author-name">Beatriz Gómez-González <a class="author-email" href="mailto:bgomezglez@gmail.com"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p>The study of the regionalized function of the blood-brain barrier at the level of brain endothelial cells and pericytes is essential to understand the biological properties and molecular mechanisms regulating this biological barrier. The isolation of</p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7651_2020_313'>more</a> </span> </div> <div class="snippet-details hidden"><p>The study of the regionalized function of the blood-brain barrier at the level of brain endothelial cells and pericytes is essential to understand the biological properties and molecular mechanisms regulating this biological barrier. The isolation of blood vessels from specific brain regions will allow to understand regional differences in susceptibility to pathological phenomena such as ischemia, traumatic brain injury, and neurodegenerative diseases, such as Alzheimer disease. Here, we propose an efficient and fast method to isolate brain endothelial cells and pericytes from a specific cerebral region. The isolated brain endothelial cells and pericytes are viable to perform conventional molecular and histological techniques such as Western blots, immunocytofluorescence, and scanning electron microscopy.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p class="collapsed"> <span>Techniques: </span>Western Blot, Recombinant Protein Expression, Confocal Microscopy, Scanning Electron Microscopy (SEM), Dissection... <a class="more-link" href="#">4 more</a> </p> <p class="expanded hidden"> <span>Techniques: </span>Western Blot, Recombinant Protein Expression, Confocal Microscopy, Scanning Electron Microscopy (SEM), Dissection, SDS-PAGE, Electrophoresis, Chemiluminescence Detection, Supercritical Drying <a class="less-link" href="#">less</a> </p> </div> </div> <div class="snippet-techniques"> <div class="antibodies"> <p><span>Antibodies: </span>Anti-claudin-5, Anti-occludin</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="views"> Downloads: <span class="green-text">2,772</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7651_2020_313</div> <div class="image-search hiddenDesktop"> </div> <div class="image-search hiddenMobile"> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Molecular Biology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7651_2021_398, Springer Protocols, 6' data-track-category='mosaic:search-page' href='/articles/10.1007/7651_2021_398'>Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Georgia Pennarossa </span> <span class="comma_space">, </span> <span class="author-name">Matteo Ghiringhelli </span> <span class="comma_space">, </span> <span class="author-name">Fulvio Gandolfi </span> <span class="comma_space">, </span> <span class="author-name">Tiziana A. Brevini <a class="author-email" href="mailto:tiziana.brevini@unimi.it"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p>Long-segment airway stenosis as well as their neoplastic transformation is life-threatening and still currently represent unsolved clinical problems. Indeed, despite several attempts, definitive surgical procedures are not presently available, and a </p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7651_2021_398'>more</a> </span> </div> <div class="snippet-details hidden"><p>Long-segment airway stenosis as well as their neoplastic transformation is life-threatening and still currently represent unsolved clinical problems. Indeed, despite several attempts, definitive surgical procedures are not presently available, and a suitable tracheal reconstruction or replacement remains an urgent clinical need. A possible innovative strategic solution to restore upper airway function may be represented by the creation of a bioprosthetic trachea, obtained through the combination of tissue engineering and regenerative medicine. Here we describe a two-step protocol for the ex vivo generation of tracheal segments. The first step involves the application of a decellularization technique that allows for the production of a naturally derived extracellular matrix (ECM)-based bio-scaffold, that maintains the macro- and micro-architecture as well as 9 the matrix-related signals distinctive of the original tissue. In the second step chondrocytes are seeded onto decellularized trachea, using a rotating bioreactor to ensure a correct scaffold repopulation. This multi-step approach represents a powerful tool for in vitro reconstruction of a bioengineered trachea that may constitute a promising solution to restore upper airway function. In addition, the procedures here described allow for the creation of a suitable 3D platform that may find useful applications, both for toxicological studies as well as organ transplantation strategies.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p><span>Techniques: </span>Tissue Engineering, Transplantation, Cell And Tissue Culture</p> </div> </div> <div class="snippet-techniques"> <div class="models"> <p><span>Models: </span>Bos taurus, Homo sapiens</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="citation"> Citations: <span class="green-text">2</span> </span> <span class="separator"> | </span> <span class="views"> Downloads: <span class="green-text">1,290</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7651_2021_398</div> <div class="image-search hiddenDesktop"> <a href="/articles/10.1007/7651_2021_398" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_398" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_398/MediaObjects/7651_2021_398_Fig1_HTML.png" alt="Decellularization protocol and macroscopic images illustrating changes in trachea color, turning from red to white, while maintaining original shape"> </a> <a href="/articles/10.1007/7651_2021_398" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_398" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_398/MediaObjects/7651_2021_398_Fig2_HTML.png" alt="Preparation of the repopulating cells, injection into the lumen of the ECM-based trachea bio-scaffold and its placement into the rotating bioreactor for long-term culture"> </a> </div> <div class="image-search hiddenMobile"> <a href="/articles/10.1007/7651_2021_398" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_398" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_398/MediaObjects/7651_2021_398_Fig1_HTML.png" alt="Decellularization protocol and macroscopic images illustrating changes in trachea color, turning from red to white, while maintaining original shape"> </a> <a href="/articles/10.1007/7651_2021_398" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_398" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_398/MediaObjects/7651_2021_398_Fig2_HTML.png" alt="Preparation of the repopulating cells, injection into the lumen of the ECM-based trachea bio-scaffold and its placement into the rotating bioreactor for long-term culture"> </a> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Molecular Biology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7651_2024_598, Springer Protocols, 7' data-track-category='mosaic:search-page' href='/articles/10.1007/7651_2024_598'>Biosynthesis of Zinc Oxide Nanoparticles Using Dried Leaves of <span class="EmphasisTypeItalic">Camellia sinensis</span>: Methods to Characterize and Assess Their Effects on Mesenchymal Stem Cell Viability</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Serap Yeşilkır Baydar <a class="author-email" href="mailto:syesilkir@gelisim.edu.tr"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> </span> <span class="comma_space">, </span> <span class="author-name">Tuba Akgül Çağlar </span> <span class="comma_space">, </span> <span class="author-name">Fatma Ebru Koç </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p><span class="EmphasisTypeItalic">Stem cell nanotechnology (SCN)</span> is an important scientific field to guide stem cell-based research of nanoparticles. Currently, nanoparticles (NPs) have a rich spectrum regarding the sources from which they are obtained (metallic, polymeric, etc.),</p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7651_2024_598'>more</a> </span> </div> <div class="snippet-details hidden"><p><span class="EmphasisTypeItalic">Stem cell nanotechnology (SCN)</span> is an important scientific field to guide stem cell-based research of nanoparticles. Currently, nanoparticles (NPs) have a rich spectrum regarding the sources from which they are obtained (metallic, polymeric, etc.), the methods of obtaining them (physical, chemical, biological), and their shape, size, electrical charge, etc. properties. It is also essential to expand green synthesis applications for the use of NPs in the field of biomedical sciences. For this purpose, there is a need to produce NPs using biological sources (plant, microorganism, algae, yeast etc.…), characterization and investigation of their effects on biological activities of stem cells. This process involves long and laborious procedures, and there may be differences in methods between individual laboratories. In this protocol, biofabrication and characterization of ZnO NPs using dried leaves of <span class="EmphasisTypeItalic">Camellia sinensis</span> is described. This experimental setup includes conventional and novel methods that can be applied to biofabricate and characterize the NPs and to examine the viability, apoptotic, and necrotic effects on human adipose tissue-derived mesenchymal stem cells (ADMSCs) in vitro.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p class="collapsed"> <span>Techniques: </span>Cell Proliferation Assay, Ultraviolet-visible Spectrophotometry, Stem Cell Culture, Flow Cytometry, Live Cell Imaging... <a class="more-link" href="#">10 more</a> </p> <p class="expanded hidden"> <span>Techniques: </span>Cell Proliferation Assay, Ultraviolet-visible Spectrophotometry, Stem Cell Culture, Flow Cytometry, Live Cell Imaging, Calcination, Light Scattering, ELISA, Trypsination, Cell Staining, Fluorescence Microscopy, Titration, Cell And Tissue Culture, Electrophoretic Light Scattering, Electron Microscopy (EM) <a class="less-link" href="#">less</a> </p> </div> </div> <div class="snippet-techniques"> <div class="models"> <p><span>Models: </span>Coturnix chinensis, Homo sapiens, Bos taurus</p> </div> </div> <div class="snippet_bottom-left"> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7651_2024_598</div> <div class="image-search hiddenDesktop"> </div> <div class="image-search hiddenMobile"> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Molecular Biology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7651_2021_412, Springer Protocols, 8' data-track-category='mosaic:search-page' href='/articles/10.1007/7651_2021_412'>High-Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Xiaohua Lei </span> <a class="author-email" href="mailto:xh.lei@siat.ac.cn"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> <span class="comma_space">, </span> <span class="author-name">Chiyuan Ma </span> <span class="comma_space">, </span> <span class="author-name">Yujing Cao </span> <span class="comma_space">, </span> <span class="author-name">Yue Xiong </span> <span>... </span><span class="author-name">Enkui Duan </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p>Human pluripotent stem cells (PSCs) are known to differentiate into almost all the blood lineage cells in vitro and hold a great promise for studying human early hematopoietic development and have a huge potential in the treatment of hematological</p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7651_2021_412'>more</a> </span> </div> <div class="snippet-details hidden"><p>Human pluripotent stem cells (PSCs) are known to differentiate into almost all the blood lineage cells in vitro and hold a great promise for studying human early hematopoietic development and have a huge potential in the treatment of hematological disorders. Although several methods of hematopoietic stem/progenitor cell (HSPC) differentiation have been developed, the HSPC yields achieved using these strategies are not yet available for clinical application. Recently, bioreactor-based devices and biochemical factors synergistically have been used to induce hematopoietic differentiation and showed a potential role in hematopoiesis. This chapter describes a protocol for using a random positioning machine bioreactor to culture human PSCs and the large-scale production of HPCs. Techniques for characterizing the differentiated cells and assessing the efficiency of hematopoietic differentiation in the bioreactor with immunostaining and flow cytometry are also presented.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p><span>Techniques: </span>Immunostaining, Flow Cytometry, Immunofluorescence, Cell And Tissue Culture, FACS</p> </div> </div> <div class="snippet-techniques"> <div class="models"> <p><span>Models: </span>Homo sapiens, Equus asinus</p> </div> </div> <div class="snippet-techniques"> <div class="antibodies"> <p><span>Antibodies: </span>Anti-human CD31, Anti-human CD34, Anti-goat</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="citation"> Citations: <span class="green-text">1</span> </span> <span class="separator"> | </span> <span class="views"> Downloads: <span class="green-text">1,261</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7651_2021_412</div> <div class="image-search hiddenDesktop"> <a href="/articles/10.1007/7651_2021_412" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_412" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_412/MediaObjects/7651_2021_412_Fig1_HTML.jpg" alt="A random positioning machine (RPM)."> </a> <a href="/articles/10.1007/7651_2021_412" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_412" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_412/MediaObjects/7651_2021_412_Fig2_HTML.jpg" alt="Mesoderm induction from human embryonic stem cells (hESCs)."> </a> <a href="/articles/10.1007/7651_2021_412" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_412" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_412/MediaObjects/7651_2021_412_Fig3_HTML.png" alt="Hemogenic endothelium induction from human embryonic stem cells (hESCs) in RPM."> </a> <a href="/articles/10.1007/7651_2021_412" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_412" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_412/MediaObjects/7651_2021_412_Fig4_HTML.png" alt="Hematopoietic progenitor cells induction from human embryonic stem cells (hESCs) in RPM."> </a> </div> <div class="image-search hiddenMobile"> <a href="/articles/10.1007/7651_2021_412" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_412" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_412/MediaObjects/7651_2021_412_Fig1_HTML.jpg" alt="A random positioning machine (RPM)."> </a> <a href="/articles/10.1007/7651_2021_412" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_412" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_412/MediaObjects/7651_2021_412_Fig2_HTML.jpg" alt="Mesoderm induction from human embryonic stem cells (hESCs)."> </a> <a href="/articles/10.1007/7651_2021_412" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7651_2021_412" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7651_2021_412/MediaObjects/7651_2021_412_Fig3_HTML.png" alt="Hemogenic endothelium induction from human embryonic stem cells (hESCs) in RPM."> </a> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Pharmacology and Toxicology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7653_2019_37, Springer Protocols, 9' data-track-category='mosaic:search-page' href='/articles/10.1007/7653_2019_37'>Cross-Species Extrapolation Using a Simplified In Vitro Tissue Explant Assay in Fish</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Bryanna Eisner </span> <span class="comma_space">, </span> <span class="author-name">Jon Doering </span> <span class="comma_space">, </span> <span class="author-name">Shawn Beitel </span> <span class="comma_space">, </span> <span class="author-name">Markus Hecker </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p>Legislation in North America and Europe, such as the Canadian Environmental Protection Act (CEPA), the US Safe Drinking Water Act (SDWA), and the European Registration, Evaluation, and Authorization of Chemicals (REACH) program, mandates the</p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7653_2019_37'>more</a> </span> </div> <div class="snippet-details hidden"><p>Legislation in North America and Europe, such as the Canadian Environmental Protection Act (CEPA), the US Safe Drinking Water Act (SDWA), and the European Registration, Evaluation, and Authorization of Chemicals (REACH) program, mandates the assessment of risks of chemicals to the environment and human health. Current testing strategies within these pieces of legislation rely on extensive animal testing and on extrapolation from standard laboratory model species to native species of relevance in local ecosystems or humans. In addition to the huge costs and large numbers of animals needed, it becomes apparent that these approaches are often not adequately predictive and, thus, protective of organisms of interest. Furthermore, there are significant ethical concerns due to the large numbers of live animals these testing efforts require. For this reason, there is a need for alternative in vitro testing approaches that enable extrapolating to in vivo results and address the lack of toxicity data for the diversity of organisms. One such in vitro method is the simplified tissue explant assay, in which tissues are excised from individuals and maintained and exposed in a culture over a few hours to several days. Tissue explants have many advantages over classic cell line-based assays, such as maintenance of paracrine cell to cell interactions, and have also shown to be less variable in some cases. Most importantly, tissue explants maintain species-specific properties that enable more realistic characterization of contaminant effects in organisms of interest. Numerous different tissues, such as liver, gonads, and lung/gill, from several taxonomic groups, including species of mammals, birds, amphibians, and fish, have been successfully cultured and exposed to contaminants. After the exposure, various biochemical endpoints as well as overall cell viability can be assessed. In this chapter, we discuss the advantages of using tissue explant assays over stable cell lines, with a focus on fish species, which has been the most commonly used taxonomic group for this purpose to date. We then outline the steps involved in conducting exposure experiments with species-specific tissue explants and provide detailed guidelines to assist in successfully carrying out and interpreting tissue explant experiments.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p><span>Techniques: </span>Explant Culture, Cytotoxicity Assay, Cell Proliferation Assay, Cell And Tissue Culture, Cytotoxicity MTT Assay</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="citation"> Citations: <span class="green-text">1</span> </span> <span class="separator"> | </span> <span class="views"> Downloads: <span class="green-text">198</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7653_2019_37</div> <div class="image-search hiddenDesktop"> <a href="/articles/10.1007/7653_2019_37" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2019_37" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2019_37/MediaObjects/7653_2019_37_Fig1_HTML.png" alt="Recommended plate format for testing cytotoxicity of chemicals"> </a> <a href="/articles/10.1007/7653_2019_37" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2019_37" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2019_37/MediaObjects/7653_2019_37_Fig2_HTML.png" alt="Transcript abundance of cytochrome P4501A (CYP1A) in white sturgeon liver tissue explants (n = 3) exposed to beta-naphthoflavone (BNF) for 3, 6, 12, 24, and 48 h."> </a> </div> <div class="image-search hiddenMobile"> <a href="/articles/10.1007/7653_2019_37" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2019_37" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2019_37/MediaObjects/7653_2019_37_Fig1_HTML.png" alt="Recommended plate format for testing cytotoxicity of chemicals"> </a> <a href="/articles/10.1007/7653_2019_37" data-track-category="mosaic:search-page" data-track="click" data-track-action="snippetimage_interact" data-track-label="10.1007/7653_2019_37" target="_blank"> <img src="https://media.springernature.com/w200/springer-static/image/chp%3A10.1007%2F7653_2019_37/MediaObjects/7653_2019_37_Fig2_HTML.png" alt="Transcript abundance of cytochrome P4501A (CYP1A) in white sturgeon liver tissue explants (n = 3) exposed to beta-naphthoflavone (BNF) for 3, 6, 12, 24, and 48 h."> </a> </div> </div> </li> <li class="SN_search-Results__searched_content" data-track-component="search-result"> <div class="SN_search-Results__searched_content_list"> <div class="snippet_source"> <div class="snippet_top-right"><span class="content-type">Protocol</span></div> <div class="snippet_top-left"> <p> <span class="source italic">Springer Protocols</span> <time datetime="" class="date-time"> (2025) </time> </p> </div> </div> <div class="snippet_source_details"> <span><b> Series: </b></span> <span class="italics">Methods In Molecular Biology</span> </div> <h3> <a class="title" target="_blank" rel="noopener" data-track="click" data-track-action='searchresult_interact' data-track-label='10.1007/7651_2020_285, Springer Protocols, 10' data-track-category='mosaic:search-page' href='/articles/10.1007/7651_2020_285'>GMP-Compatible, Xeno-Free Culture of Human Induced Mesenchymal Stem Cells</a> </h3> <div class="author-info"> <span class="authors"> <span class="author-name">Giuseppe Maria Peppo <a class="author-email" href="mailto:gmdepeppo@nyscf.org"> <img class="author-email-icon" src="/assets/images/icons/email.png" alt="Email to Author"/> </a> </span> </span> </div> <div class="snippet"> <div> <div class="trimmedText"><p>Mesenchymal stem cells (MSCs) have been used in therapies owing to their regenerative potential, paracrine regulatory effects, and immunomodulatory activity. To foster commercialization and implementation of stem cells treatments, researchers have</p>  <span class="read-more">… <a href="#" class="more-link" data-track="click" data-track-action="snippet_interact" data-track-category="mosaic:search-page" data-track-label='10.1007/7651_2020_285'>more</a> </span> </div> <div class="snippet-details hidden"><p>Mesenchymal stem cells (MSCs) have been used in therapies owing to their regenerative potential, paracrine regulatory effects, and immunomodulatory activity. To foster commercialization and implementation of stem cells treatments, researchers have recently derived MSCs from human induced pluripotent stem cells (iMSCs). For therapeutic applications, human iMSCs must be produced in xeno-free culture conditions and following procedures that are compatible with the principles of Good Manufacturing Practice.</p> <span class="read-less"> <a href="#" class="less-link">less</a></span> </div> </div> </div> <div class="snippet-techniques"> <div class="techniques"> <p><span>Techniques: </span>Cell And Tissue Culture, Cryopreservation</p> </div> </div> <div class="snippet-techniques"> <div class="models"> <p><span>Models: </span>Homo sapiens</p> </div> </div> <div class="snippet_bottom-left"> <div class="views-citations"> <span class="citation"> Citations: <span class="green-text">1</span> </span> <span class="separator"> | </span> <span class="views"> Downloads: <span class="green-text">1,088</span> </span> </div> <div class="version-video"> </div> </div> <div class="p_doi" hidden>10.1007/7651_2020_285</div> <div class="image-search hiddenDesktop"> </div> <div class="image-search hiddenMobile"> </div> </div> </li> </ul> <div class="pagination_bottom" data-track-component="pagination"> <div class="pagination-bar"> <div class="pagination-bar-form" id="pagination-form" autocomplete="off"> <span class="prev span-prev"></span> <span class="page-nr-wrapper"> Page <input id="pageNo" name="Page no" title="Page no" class="page-number" value=1 type="number" min="1" max="7822" data-track="click" data-track-category="mosaic:search-page" data-track-action="pagination_interact" data-track-label='1' required pattern="\d*" aria-label="page-number"> <input id="pageNumber" class="InputText" type="hidden" name="pageNumber" value=1 /> of <span id="totalNumberOfPages" class="pagination__number-of-pages">7822</span> </span> <a href="#" class="next anchor-next pagination-next" title="next" data-track="click" data-track-category="mosaic:search-page" data-track-action="pagination_interact" data-track-label='1'> </a> </div> <div class="pagination-error-msg" style="display:none">Invalid page number</div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </main> <footer class="SN_footer SN_resultsPage"> <div class="SN_container"> <div class="SN_footer__nav"> <div class="SN_footer__col column1" role="navigation" aria-label="column1"> <div class="SN_footer__col_head">About Protocols and Methods</div> <ul> <li> <a href="http://www.nature.com/nmeth/index.html" target="_blank" rel="noopener">Nature Methods</a> </li> <li> <a href="https://www.nature.com/nprot/" target="_blank" rel="noopener">Nature Protocols</a> </li> <li> <a href="https://www.nature.com/nrmp/" target="_blank" rel="noopener"> Nature Reviews Methods Primers</a> </li> <li> <a href="https://www.springernature.com/gp/librarians/products/product-types/database/springerprotocols" target="_blank" rel="noopener">Springer Protocols</a> </li> </ul> </div> <div class="SN_footer__col column2" role="navigation" aria-label="column2"> <div class="SN_footer__col_head">About Springer Nature</div> <ul> <li> <a href="http://www.springernature.com/" target="_blank" rel="noopener">Springer Nature</a> </li> <li> <a href="mailto:experiments@springernature.com">Contact Us</a> </li> </ul> </div> <div class="SN_footer__col column3" role="navigation" aria-label="column3"> <div class="SN_footer__col_head">Librarian links</div> <ul> <li> <a href="https://www.springernature.com/gp/librarians/tools-services/implement/remote-access" target="_blank" rel="noopener">Implement Remote Access </a> </li> <li> <a href="http://www.springernature.com/gb/librarians/manage-your-account/marc-records/title-list-downloader" target="_blank" rel="noopener">MARC Records</a> </li> <li> <a href="https://librarian.springernature.com" target="_blank" rel="noopener">Springer Nature Librarian Portal</a> </li> </ul> </div> <div class="SN_footer__col column4" role="navigation" aria-label="column4"> <div class="SN_footer__col_head">Legal</div> <ul> <li> <a href="/privacy-policy">Privacy Policy</a> </li> <li> <a href="/cookie-policy">Cookie Policy</a> </li> <li><button class="cmp-manage-cookies" data-cc-action="preferences">Manage Cookies/Do Not Sell My Info</button></li> <li> <a href="/legal-notice">Legal Notice</a> </li> <li> <a href="/impressum-imprint">Impressum/Imprint</a> </li> <li> <a href="/external-data-sources">External Data Sources</a> </li> </ul> </div> </div> </div> <div class="SN_footer__branding"> <div class="SN_container"> <div class="SN_Footer__brandingLogo"> <img srcset="/assets/images/sn_logo_footer.svg" src="/assets/images/sn_logo_footer.png" alt="Springer Nature"> </div> <div class="SN_footer__branding-copyright"> © <span class="copyright-year"></span> Springer Nature Switzerland AG. 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