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as a Method * define function as object methods * @type {Object} */ window.digitalData = { user: {}, page: {}, merchandising: {}, getQueryParam : function (param) { var index, key, value, hash; var hashes = window.location.href.slice(window.location.href.indexOf('?') + 1).split('&'); for (index = 0; index < hashes.length; index++) { hash = hashes[index].split('='); key = hash[0]; value = hash[1]; if (key.toUpperCase() === param.toUpperCase()) return value; } return ''; }, getValOnce: function (value, cookieName, expiry, timeUnit) { var currentDate = new Date; var currentCookieValue; // timeUnit of 'm' is minutes represented in milliseconds // thus 86400000 = 24 hours = 1 day var interval = timeUnit == 'm' ? 60000 : 86400000; cookieName = cookieName ? cookieName : 'digitaldata_gvo'; value = value ? value : ''; expiry = expiry ? expiry : 0; currentCookieValue = this.getCookie(cookieName); if (value) { currentDate.setTime(currentDate.getTime() + expiry * interval); this.setCookie(cookieName, value, expiry == 0 ? 0 : currentDate); } return value == currentCookieValue ? '' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); var refReg = document.referrer; var referrerDomain = refReg.match(/:\/\/([^/]+)\//); if (referrerDomain !== null && referrerDomain[1].indexOf(masterDomain) == -1) { this.merchandising.referrer = referrerDomain; } } document.addEventListener('registerMerchandisingData', function (event) { for (var key in event.detail) { console.log("Listener data copied", key, event.detail[key] ); digitalData.merchandising[key] = event.detail[key]; } // Map some analytics attributes to merchandising session attributes digitalData.merchandising.sessionId = digitalData.merchandising.analyticsVisitorID; digitalData.merchandising.s_vi_low = digitalData.merchandising.analyticsVisitorIDLow; digitalData.merchandising.s_vi_high = digitalData.merchandising.analyticsVisitorIDHigh; console.log("digitalData: Captured registerMerchandisingData event"); digitalData.merchandisingDataInitted = true; // Call emailCampaignRecord if the digitalDataAnalyticsListener object is loaded if (typeof digitalDataAnalyticsListener != "undefined") { console.log("digitalData: Calling email campaign record"); digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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eBioscience™</h1> <div class="details-hero" onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationPublishedFigures(event, this)"> <a class="link published-link animated-scroll " href="#image-gallery" onclick="window.THERMO_FISHER.pdp.inp.publishedFiguresHero(event, this)"> <svg class="svg-icon published-figures-icon"> <use href="#icon-published-figures"></use> </svg> 11 Published Figures </a> </div> <div class="details-hero"> <a class="link references-link animated-scroll " href="#references-component-id" onclick="window.THERMO_FISHER.pdp.inp.referencesHero(event, this)"> <svg class="svg-icon references-icon"> <use href="#icon-references"></use> </svg> 11 References </a> </div> <div class="details-hero"> <a class="btn hero-btn hero-view-other" href="/antibody/primary/target/cd163"> View all (131) CD163 antibodies </a> </div> </div> <div class="links-ab to-show-links-in-sm"> <div class="container"> <div class="link-container"> <a class="link-antibody" 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ng-class="{'is-loaded':$ctrl.isLoaded}"> <div id="tabName" tab-name-value="changeTabValue"></div> <div id="originalProductLink" product-link-value="originalProductLinkValue"></div> <div class="gal-cntr container" tfs-media-gallery sku="'46-1631-82'" media-items="{'Antibody Testing Data':[{'sku':'46-1631-82','imageId':'129104','imageUrl':'https://www.thermofisher.com/antibody/images/650/46-1631-82-CD163-antibody-flow-cytometry-062217lsrds-001_20170728195150.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/46-1631-82-CD163-antibody-flow-cytometry-062217lsrds-001_20170728195150.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/46-1631-82-CD163-antibody-flow-cytometry-062217lsrds-001_20170728195150.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/46-1631-82-CD163-antibody-flow-cytometry-062217lsrds-001_20170728195150.jpg?time\u003d20240507','sortOrder':'1','description':'BALB/c mouse splenocytes were stained with F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-48-4801) and 0.125 µg of Rat IgG2a kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4321) (left) or 0.125 µg of CD163 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-1631) (right). Total cells were used for analysis.','shortDescription':'BALB/c mouse splenocytes were stained with F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-48-4801) and 0.125 µg of Rat IgG2a kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4321) (left) or 0.125 µg of CD163 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-1631) (right). Total cells were used for analysis.','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','appName':'Flow Cytometry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'46-1631-82-CD163-antibody-flow-cytometry-062217lsrds-001_20170728195150.jpg','benchSciPubmedId':'0'}],'Published Figures':[{'sku':'46-1631-82','imageId':'1018318','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24810_12935_2022_2769_Fig2_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24810_12935_2022_2769_Fig2_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24810_12935_2022_2769_Fig2_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24810_12935_2022_2769_Fig2_HTML.jpg?time\u003d20240507','sortOrder':'2','description':'Fig. 2 ADMA Drives Macrophages Towards an M2-like subtype. RAW264.7 cells were treat-ed with DPBS (control) or ADMA (5 mM) for 3 days. RNA was collected and expression of M1- and M2-related genes was analyzed. Many M2 related markers were strikingly induced, in-cluding ARG ( A ), CD163 ( B ) and CD206 ( C ) while the M1 related markers NOS ( D ) and TNFalpha ( E ) were slightly or not impacted by ADMA. However, some other M2 related markers were downregulated such as IL10 ( F ) and Dectin-1 ( G ). Additionally, the chemotactic receptor CCR2 was also downregulated by ADMA ( H ). The most dramatically upregulated M2 markers (CD163 and CD206) by RNA expression were validated at the protein level by flow cytometry ( I ). Data are presented as mean +- SD. (*p \u003c 0.05, *** p\u003c0.001, ****p\u003c0.0001), N\u003d4 for all groups','shortDescription':'Fig. 2 ADMA Drives Macrophages Towards an M2-like subtype. RAW264.7 cells were treat-ed with DPBS (control) or ADMA (5 mM) for 3 days. RNA was collected and expression of M1- and M2-related genes was analyzed. Many M2 related markers were strikingly induced, in-cluding ARG ( A ), CD163 ( B ) and CD206 ( C ) while the M1 related markers NOS ( D ) and TNFalpha ( E ) were slightly or not impacted by ADMA. Howe... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'Nov 15, 2022, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Cancer cell international','journalText':'Cancer cell international 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24810_12935_2022_2769_Fig2_HTML.jpg','benchSciPubmedId':'36376929'},{'sku':'46-1631-82','imageId':'938242','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_15351_nihms-1633903-f0013.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_15351_nihms-1633903-f0013.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_15351_nihms-1633903-f0013.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_15351_nihms-1633903-f0013.jpg?time\u003d20240507','sortOrder':'3','description':'Extended Data Figure 7: Early profibrotic macrophages express M2a markers and are lost upon anti-CSF1 treatment. ( a ) Scatter plot showing individual values and mean +- SEM of the number of F4-80 positive cells per field of view in IgG1 treated (n \u003d 6) or anti-CSF1 treated (n \u003d 4) PyMT tumors from 8-week-old mice. ( b-c ) Scatter plots showing individual values and mean +- SEM of the percentage of F4/80 positive cells expressing markers MHCII and CD86 ( b ) and markers CD163 and Relmalpha ( c ) in IgG1 treated (n \u003d 6) or anti-CSF1 treated (n \u003d 4-5) PyMT tumors from 8-week-old mice. ( d-g ) Scatter plots showing individual values and mean +- SEM of the number of cells per field of view (FOV) of other non-macrophage immune populations CD11b + F4-80 - ( d ), MHCII hi F4-80 - ( e ), CD45 + CD4 + ( f ), and CD45 + CD8 + ( g ) in IgG1 (n \u003d 4 e,g ; n \u003d 5 d,f ) and anti-CSF1 (n \u003d 6) treated mice. Statistical analysis ( a-g ) was performed using two-tailed Mann-Whitney U test ( a ) *p \u003d 0.019 ( b ) MHCII hi p \u003d 0.7190, CD86 + p \u003d 0.4286 ( c ) CD163 + *p \u003d 0.0303, Relmalpha + p \u003d 0.0381 ( d ) p \u003d 0.9719 ( e ) p \u003d 0.1524 ( f ) p \u003d 0.3074 ( g ) p \u003d 1.000. ( h ) Heat map of selected differentially expressed genes from macrophages sorted from 8-week and 11-week PyMT tumors. ( i ) Representative images from 8-week-old IgG1 treated and anti-CSF1 treated PyMT mice stained for cytokeratin 8/18 (green), PDGFRalpha (magenta), vimentin (yellow), and DAPI (blue). Scale bar is 100 mum. ( j ) Scatt','shortDescription':'Extended Data Figure 7: Early profibrotic macrophages express M2a markers and are lost upon anti-CSF1 treatment. ( a ) Scatter plot showing individual values and mean +- SEM of the number of F4-80 positive cells per field of view in IgG1 treated (n \u003d 6) or anti-CSF1 treated (n \u003d 4) PyMT tumors from 8-week-old mice. ( b-c ) Scatter plots showing individual values and mean +- SEM of the percentage of F4/80 po... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Immunohistochemistry (IHC)','title':'CD163 Antibody (46-1631-82) in IHC','appAbv':'IHC','dateTime':'Apr 1, 2021, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Nature materials','journalText':'Nature materials 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_15351_nihms-1633903-f0013.jpg','benchSciPubmedId':'33257795'},{'sku':'46-1631-82','imageId':'1131601','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29209_gr5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29209_gr5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29209_gr5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29209_gr5.jpg?time\u003d20240507','sortOrder':'4','description':'Accumulation of hepatic myeloid cells in tumors of Shp2 DeltaHK mice. ( A ) FACS analysis to evaluate ratios of total macrophages in CD45 cells in the livers. ( B ) FACS analysis to determine the ratios of KCs, non-KCs, MDSCs, and DCs in hepatic CD45 cells (n \u003d 8). ( C ) Representative immunostaining and quantification of TAMs in the liver. Magnification, x100; scale bar : 100 mum. ( D ) FACS analysis to quantify the ratios of Ki67 non-KC macrophages in hepatic CD45 cells. ( E ) Quantitative reverse-transcription PCR to measure mRNA levels of cytokines and other genes as shown. (n \u003d 8 per group) ( F ) FACS analysis to quantify the ratios of CD11b DCs in hepatic CD45 cells. Flow cytometric analysis to show ratios of ( G ) CD163 cells, ( H ) CD206 cells, ( I ) MHCII cells, ( J ) mean fluorescence intensity of CD11b, and ( K ) F4/80 in KCs or non-KC macrophages in the same mouse. Phenotypic comparison between KCs and non-KC macrophages were evaluated by a paired-samples t test. * P \u003c .05, ** P \u003c .01, and *** P \u003c .001. ADM, adrenomedullin; Arg-1, arginase-1; bFGF, basic fibroblast growth factor; FMI, median fluorescence intensity; HGF, hepatocyte growth factor; iNOS, inducible nitric oxide synthase; Mac, macrophage; MMP, matrix metalloproteinase; PGF, placental growth factor; TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.','shortDescription':'Accumulation of hepatic myeloid cells in tumors of Shp2 DeltaHK mice. ( A ) FACS analysis to evaluate ratios of total macrophages in CD45 cells in the livers. ( B ) FACS analysis to determine the ratios of KCs, non-KCs, MDSCs, and DCs in hepatic CD45 cells (n \u003d 8). ( C ) Representative immunostaining and quantification of TAMs in the liver. Magnification, x100; scale bar : 100 mum. ( D ) FACS analysis to ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'May 22, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Cellular and molecular gastroenterology and hepatology','journalText':'Cellular and molecular gastroenterology and hepatology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29209_gr5.jpg','benchSciPubmedId':'36828281'},{'sku':'46-1631-82','imageId':'1131602','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29209_gr7.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29209_gr7.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29209_gr7.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29209_gr7.jpg?time\u003d20240507','sortOrder':'5','description':'Figure 7 FACS analysis to show membrane marker expression of KCs and non-KC macrophages. Representative flow cytometric images to show expression of ( A ) CD163, ( B ) CD206, ( C ) MHCII, ( D ) CD11b, and ( E ) F4/80 in KCs and non-KC macrophages in the liver. FSC-A, forward scatter-area.','shortDescription':'Figure 7 FACS analysis to show membrane marker expression of KCs and non-KC macrophages. Representative flow cytometric images to show expression of ( A ) CD163, ( B ) CD206, ( C ) MHCII, ( D ) CD11b, and ( E ) F4/80 in KCs and non-KC macrophages in the liver. FSC-A, forward scatter-area.','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'May 22, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Cellular and molecular gastroenterology and hepatology','journalText':'Cellular and molecular gastroenterology and hepatology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29209_gr7.jpg','benchSciPubmedId':'36828281'},{'sku':'46-1631-82','imageId':'1021244','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24811_nihms-1846963-f0002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24811_nihms-1846963-f0002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24811_nihms-1846963-f0002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24811_nihms-1846963-f0002.jpg?time\u003d20240507','sortOrder':'6','description':'Figure 2. Immuno-profiling revealed an overall immune-suppressive TME in Tmem30 -/- (PS out ) tumors (A-G) Flow cytometry quantitative analysis of TILs at the endpoint (day 16). Shown are no infiltrating differences in CD45 + CD11b - CD3 + CD4 + T cells (B), CD45 + CD11b - CD3 + CD8 + T cells (C), CD45 + CD11b - CD3 + CD4 + CD25 + Foxp3 + Tregs (D), and CD45 + CD11b + Ly6G - Ly6C + Mo-MDSC (F); but more infiltrating cells in CD45 + CD11b + Ly6G - Ly6C - F4/80 TAMs (E) and CD45 + CD11b + Ly6C - Ly6G + PMN-MDSC (G). (H) Representative flow plots of MHC II expression in TAMs. (I) Flow cytometry analysis of MHC II + and MHC II - TAM distribution (n \u003d 5). (J and K) Flow cytometry quantitative analysis of MHC II + and MHC II - TAMs (n \u003d 5). (L) RNA-seq analysis of sorted TAMs in WT or PS out ( Tmem30 -/- ) TMEs. Shown is the Z score of gene expression (n \u003d 4 for PS out and n \u003d 3 for WT). (M and N) Geometric mean fluorescence intensity (gMFI) of CD163 (M) and CD206 (N) shows higher expression on TAMs (n \u003d 5). (O-Q) qPCR analysis of MHC II, CD163, and CD206 in MHC II + and MHC II - TAMs from both MC38-WT and Tmem30a -/- TMEs (n \u003d 4). (R and S) gMFI of PD-1 (R) and Tim-3 (S) shows lower expression on CD8 + T cells (n \u003d 5). (T) Flow cytometry analysis of PD-1 and TIM-3 CD8 + T cell distribution (n \u003d 5). (U) Representative flow plots and analysis of tetramer + in CD8 + T cells (n \u003d 5). Data were representative of at least two independent experiments (except RNAseq). Data represent mean','shortDescription':'Figure 2. Immuno-profiling revealed an overall immune-suppressive TME in Tmem30 -/- (PS out ) tumors (A-G) Flow cytometry quantitative analysis of TILs at the endpoint (day 16). Shown are no infiltrating differences in CD45 + CD11b - CD3 + CD4 + T cells (B), CD45 + CD11b - CD3 + CD8 + T cells (C), CD45 + CD11b - CD3 + CD4 + CD25 + Foxp3 + Tregs (D), and CD45 + CD11b + Ly6G - Ly6C + Mo-MDSC (F); but more inf... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'Nov 1, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Cell reports','journalText':'Cell reports 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24811_nihms-1846963-f0002.jpg','benchSciPubmedId':'36323258'},{'sku':'46-1631-82','imageId':'1021232','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24809_12964_2022_957_Fig8_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24809_12964_2022_957_Fig8_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24809_12964_2022_957_Fig8_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24809_12964_2022_957_Fig8_HTML.jpg?time\u003d20240507','sortOrder':'7','description':'Fig. 8 AP000439.2-deleted exosome suppressed tumor growth. Xenograft tumor was established by injecting Caki-1 cells, in the meantime, mice treated with AP000439.2-deleted exosome or NC exosome via tail vein injection. A Xenograft tumor images from each mouse. B Body weight curve of mice. C Tumor weight of mice from two groups. D Tumor volume curve of mice from two groups. E The proportion of M1 (F4/80 + NOS2 + ) and M2 (F4/80 + CD163 + ) macrophages in tumor tissues by flow cytometry. *p \u003c 0.05, **p \u003c 0.01. Two-tailed Student\u0026apos;s t-test','shortDescription':'Fig. 8 AP000439.2-deleted exosome suppressed tumor growth. Xenograft tumor was established by injecting Caki-1 cells, in the meantime, mice treated with AP000439.2-deleted exosome or NC exosome via tail vein injection. A Xenograft tumor images from each mouse. B Body weight curve of mice. C Tumor weight of mice from two groups. D Tumor volume curve of mice from two groups. E The proportion of M1 (F4/80 + NO... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'Sep 24, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Cell communication and signaling : CCS','journalText':'Cell communication and signaling : CCS 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24809_12964_2022_957_Fig8_HTML.jpg','benchSciPubmedId':'36153596'},{'sku':'46-1631-82','imageId':'988907','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24585_gr9.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24585_gr9.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24585_gr9.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24585_gr9.jpg?time\u003d20240507','sortOrder':'8','description':'Figure 9 M2 macrophages are required for normal as well as ON101-enhanced diabetic wound healing. ( a-c ) Subcutaneous injection of m-Clo or m-Enc during normal wound healing in C57BL/6 mice. ( a ) Schematic; n \u003d 4 mice per group. ( b ) Wound recovery rate, calculated as the percentage change from the original size (day 0) and images of wounds at the indicated times. ( c )Biopsies from the point of wounding were analyzed by flow cytometry to detect the proportions of M2 macrophages (F4/80 + /CD163 + ) at indicated time point. Data represent the percentages of F4/80 + or CD163 + macrophages. ( d-h ) M2 macrophage dependence of ON101-promoted diabetic wound healing. m-Clo/m-Enc. ( d ) Schematic; n \u003d 4 mice per group. ( e ) Wound recovery rate (percentage change from day 0) and images of wounds at the indicated times for each treatment group. ( f ) H\u0026E staining represents re-epithelization (dotted line) and the WB in the indicated treatment. ( g ) FACS analysis of the proportions of M2 macrophages (F4/80 + /CD163 + ) in wound surroundings. ( h ) IHC staining of wound biopsies with indicated antibodies on day 9 after wounding. # denotes wound bed. Bar \u003d 250 mum. Data represent means +- SEM. P values in b and e were analyzed using two-way repeated-measures ANOVA, wheares that in g was analyzed using Student\u0027s t -test. * P \u003c 0.05, ** P \u003c 0.01, and *** P \u003c 0.001. alpha-SMA, alpha-smooth muscle actin; m-Enc, m-Encapasome; IHC, immunohistochemistry; iNOS, inducible nitrate oxide synth','shortDescription':'Figure 9 M2 macrophages are required for normal as well as ON101-enhanced diabetic wound healing. ( a-c ) Subcutaneous injection of m-Clo or m-Enc during normal wound healing in C57BL/6 mice. ( a ) Schematic; n \u003d 4 mice per group. ( b ) Wound recovery rate, calculated as the percentage change from the original size (day 0) and images of wounds at the indicated times. ( c )Biopsies from the point of wounding... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'Sep 1, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'JID innovations : skin science from molecules to population health','journalText':'JID innovations : skin science from molecules to population health 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24585_gr9.jpg','benchSciPubmedId':'36017415'},{'sku':'46-1631-82','imageId':'938260','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19182_WJSC-13-1762-g004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19182_WJSC-13-1762-g004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19182_WJSC-13-1762-g004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19182_WJSC-13-1762-g004.jpg?time\u003d20240507','sortOrder':'9','description':'Figure 4 IF-C2C12-Exos induced M1 macrophage polarization in vitro . A: Images of macrophages cultured with different concentrations of C2C12-Exos for 2 d (1 x 10 9 , 1 x 10 10 , 2 x 10 10 /medium). Scale bar \u003d 200 mum or 100 mum; B: Immunofluorescence localization of CD86 (red), marker of M1 and CD206 (green), marker of M2 after culture with different concentrations of C2C12-Exos for 2 d. Scale bar \u003d 90 mum. Arrows indicate CD206 or CD86 positive cells; C: Representative flow cytometry plots showing the percentages of M1 (CD86 + CD4/80+) and M2 (CD163 + CD4/80+) phenotype in macrophages after culture with different concentrations of C2C12-Exos for 24 h; D: Quantification of flow cytometry data ( n \u003d 3); E: M2 macrophages percentage. Data are presented as mean +- SD. a P \u003c 0.05; b P \u003c 0.01. NS: Not significant.','shortDescription':'Figure 4 IF-C2C12-Exos induced M1 macrophage polarization in vitro . A: Images of macrophages cultured with different concentrations of C2C12-Exos for 2 d (1 x 10 9 , 1 x 10 10 , 2 x 10 10 /medium). Scale bar \u003d 200 mum or 100 mum; B: Immunofluorescence localization of CD86 (red), marker of M1 and CD206 (green), marker of M2 after culture with different concentrations of C2C12-Exos for 2 d. Scale bar \u003d 90 mu... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'Nov 26, 2021, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'World journal of stem cells','journalText':'World journal of stem cells 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19182_WJSC-13-1762-g004.jpg','benchSciPubmedId':'34909122'},{'sku':'46-1631-82','imageId':'938248','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17688_41434_2021_269_Fig5_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17688_41434_2021_269_Fig5_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17688_41434_2021_269_Fig5_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17688_41434_2021_269_Fig5_HTML.jpg?time\u003d20240507','sortOrder':'10','description':'Fig. 5 Expression of AAT in human MPhi. A Representative AAT western blot analysis of human MPhi supernatant and MPhi lysates. Human serum was used as a positive control. Vinculin band in cellular lysates served as loading control. B ELISA quantification of AAT secretion by human MPhi. n \u003d 3. C Representative May-Grunwald-Giemsa staining of MPhi cytospins. Scale bar \u003d 20 mum. D Representative flow cytometric analysis of myeloid and MPhi-specific surface marker expression by human MPhi. E GM-CSF uptake from cell culture medium by MPhi. A well without cells was used as a negative control. n \u003d 3. All data represent independent cell isolations and transductions.','shortDescription':'Fig. 5 Expression of AAT in human MPhi. A Representative AAT western blot analysis of human MPhi supernatant and MPhi lysates. Human serum was used as a positive control. Vinculin band in cellular lysates served as loading control. B ELISA quantification of AAT secretion by human MPhi. n \u003d 3. C Representative May-Grunwald-Giemsa staining of MPhi cytospins. Scale bar \u003d 20 mum. D Representative flow cytometri... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'Sep 1, 2021, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Gene therapy','journalText':'Gene therapy 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17688_41434_2021_269_Fig5_HTML.jpg','benchSciPubmedId':'34276045'},{'sku':'46-1631-82','imageId':'844317','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_13068_ijms-22-03188-g006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_13068_ijms-22-03188-g006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_13068_ijms-22-03188-g006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_13068_ijms-22-03188-g006.jpg?time\u003d20240507','sortOrder':'11','description':'Figure 6 M.C increased the ratio of M2a and M2c macrophages. Spleen-derived cells from the sarcopenia mouse model were assessed by flow cytometry. ( A , B ) Representative flow cytometry (FACS) dot plots of CD206, CD163 in CD11b + F4/80 + , and CD45 + cells. CD206 - CD163 - M1 macrophages; CD206 + CD163 - M2a macrophages; CD206 + CD163 + M2c macrophages; ( C - E ) graphs of M1 macrophage, M2a macrophage, and M2c macrophage, presented as % of CD11b + F4/80 + cells; ( F ) the CD4 + /CD8 ratio of CD45 + cells in splenocytes, presented as the mean +- SEM ( n \u003d 4/group). * p \u003c 0.05, ** p \u003c 0.01, *** p \u003c 0.001 vs. CIS+PBS, using a Bonferroni post hoc test after one-way ANOVA.','shortDescription':'Figure 6 M.C increased the ratio of M2a and M2c macrophages. Spleen-derived cells from the sarcopenia mouse model were assessed by flow cytometry. ( A , B ) Representative flow cytometry (FACS) dot plots of CD206, CD163 in CD11b + F4/80 + , and CD45 + cells. CD206 - CD163 - M1 macrophages; CD206 + CD163 - M2a macrophages; CD206 + CD163 + M2c macrophages; ( C - E ) graphs of M1 macrophage, M2a macrophage, an... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'Mar 20, 2021, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'International journal of molecular sciences','journalText':'International journal of molecular sciences 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_13068_ijms-22-03188-g006.jpg','benchSciPubmedId':'33804803'},{'sku':'46-1631-82','imageId':'938231','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19211_DDDT-15-803-g0005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19211_DDDT-15-803-g0005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19211_DDDT-15-803-g0005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19211_DDDT-15-803-g0005.jpg?time\u003d20240507','sortOrder':'12','description':'Figure 5 CNT facilitated macrophage polarizing towards M2 phenotype in vitro. ( A ) Flow cytometry analysis and quantification of the percentage of CD163 positive cells. ( B ) qPCR results of the relative expressions of M2 markers, including Arg1, Mrc1, Retnla, and Chi3l3. ( C ) ELISA assays of the levels of IL-6, TNF-alpha and IL-10 in cell supernatants. Data were expressed as mean +- SD. Abbreviations: Con, control; CNT-L, low dose of CNT; CNT-H, high dose of CNT.','shortDescription':'Figure 5 CNT facilitated macrophage polarizing towards M2 phenotype in vitro. ( A ) Flow cytometry analysis and quantification of the percentage of CD163 positive cells. ( B ) qPCR results of the relative expressions of M2 markers, including Arg1, Mrc1, Retnla, and Chi3l3. ( C ) ELISA assays of the levels of IL-6, TNF-alpha and IL-10 in cell supernatants. Data were expressed as mean +- SD. Abbreviations: Co... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD163 Antibody in Flow Cytometry (Flow)','title':'CD163 Antibody (46-1631-82) in Flow','appAbv':'Flow','dateTime':'Oct 6, 2021, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Drug design, development and therapy','journalText':'Drug design, development and therapy 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19211_DDDT-15-803-g0005.jpg','benchSciPubmedId':'33654384'}],'Advanced Verification':[]}" image-displayed-id="'129104'" product-sub-type="'antibody_primary'" prod-type="'antibody'" page-type="PDP" product-id="'46-1631-82'"> <div class="tabs"> <ul class="nav nav-pills"> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Antibody Testing Data"> <a class="tab-text"> Antibody Testing Data (1) </a> </li> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Published Figures"> <a class="tab-text"> Published Figures (11) </a> </li> </ul> <div gallery-flt class="gal-flt" is-with-abbr="true" enable-mobile-flt="true" mobile-result-count="true" filter-data="$ctrl.applicationsList" advanced-filter-data="$ctrl.advancedApplicationsList" filter-text="Application" event-name="{{$ctrl.fltAppEvent}}"> </div> </div> <div class="cntnt" ng-swipe-right="$ctrl.clickLeftArrow()" ng-swipe-left="$ctrl.clickRightArrow()"> <div class="nav-arrow" ng-if="!$ctrl.isMobileSmallTabletScreen()" onclick="window.THERMO_FISHER.inp.onClickOfLeftArrow(event, this)"> <div tabindex="0" class="gallery-left-arrows icon-gallery-arrow-left" > </div> </div> <div class="media-text"> <div class="media-display-mobile" ng-if="$ctrl.isMobileSmallTabletScreen()"> <div class="nav-arrow" onclick="window.THERMO_FISHER.inp.onClickOfLeftArrow(event, this)"> <div tabindex="0" class="gallery-left-arrows icon-gallery-arrow-left" > </div> </div> <div class="media-display"> <img 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xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px" viewBox="0 0 22 22" style="enable-background:new 0 0 22 22;" xml:space="preserve"> <style type="text/css"> .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#222222;} </style> <title>Group 53</title> <desc>Created with Sketch.</desc> <g id="Symbols"> <g id="rollover-expand-icon" transform="translate(-8.000000, -8.000000)"> <g id="Group-48"> <g id="Group-53" transform="translate(8.000000, 8.000000)"> <g id="Group-49" transform="translate(0.000000, 0.000000)"> <g id="Group-54"> <g id="Group-55" transform="translate(0.000000, -0.000000)"> <path id="Fill-1" class="st0" d="M21.9,14.6h-2.2c0,1,0,1.9,0,2.9c0,0.2,0,0.4,0,0.6c-0.1-0.1-0.2-0.2-0.3-0.2 c-1.3-1.3-2.7-2.7-4-4l-1.6,1.5c1.3,1.4,2.7,2.7,4,4.1c0.1,0.1,0.1,0.2,0.3,0.3c-0.2,0-0.3,0-0.4,0c-1,0-2.1,0-3.1,0V22 c2.3,0,4.8,0,7.1,0.1c0.1,0,0.2,0,0.3,0c0-0.5-0.1-0.9-0.1-1.3C21.9,18.7,21.9,16.6,21.9,14.6z"/> <path id="Fill-4" class="st0" 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<span class="current-img"> 1 </span> / <span class="img-total-count"> 12</span></p> </div> <div class = "verification-certificate hide" > <span class="icon-certification"></span> <span class="adv-cert-text">{{ $ctrl.currentElement.advancedVerification.fullName }}</span> <div class="cert-provider">{{ $ctrl.currentElement.advancedVerification.text }}</div> </div> <h2 class="product-name" ng-show="!$ctrl.isVideo"> CD163 Antibody (46-1631-82) in Flow </h2> <div ng-show="!$ctrl.isVideo"> <div class="image-desc"> <div class="product-desc-text viewing-more-long-description"> <span class="image-journal-text" > </span> <span class="short-desc"> BALB/c mouse splenocytes were stained with F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-48-4801) and 0.125 µg of Rat IgG2a kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4321) (left) or 0.125 µg of CD163 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-1631) (right). Total cells were used for analysis. </span> <span class="verification-info hide" ng-if="$ctrl.hasVerificationMethod"> <a rel="nofollow" ng-href="{{$ctrl.currentElement.advancedVerification.uri}}" >{{ $ctrl.currentElement.advancedVerification.fullName }} validation info.</a> </span> <a href="#" class="images-view-more-text hide" ng-if="$ctrl.showViewMore" onclick="window.THERMO_FISHER.inp.openImageGalleryModal(event, this)"> View more </a> </div> </div> <div class="img-desc-footer"> <div class="benchsci-pubmed-footer"> <div ng-cloak ng-if="$ctrl.currentElement.benchsci || $ctrl.sourceType === '3'" class="images-benchsci-footer"> <div class="images-benchsci-link" tabindex="0"> <span>Published figure supplied by</span> <span class="icon-benchsci-logo"> <svg id="Layer_1" data-name="Layer 1" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 89.75 23.21"><defs><style>.cls-1{fill:#222222;}</style></defs><title>benchsci-logo</title><g id="logo-blue-copy-3"><g id="logo-blue-copy-2"><path id="path14" class="cls-1" d="M34.54,11.49a2.45,2.45,0,0,0,1.59-2.34c0-1.69-1.21-2.94-3.32-2.94H28.88v10.9h4.21a3.06,3.06,0,0,0,3.37-3A2.57,2.57,0,0,0,34.54,11.49ZM31,8h1.49c1,0,1.57.53,1.57,1.36a1.36,1.36,0,0,1-1.55,1.37H31Zm1.72,7.35H31V12.47h1.75a1.42,1.42,0,0,1,1.62,1.46C34.34,14.79,33.72,15.34,32.69,15.34Z"/><path id="path16" class="cls-1" d="M41.34,9.32a3.8,3.8,0,0,0-3.72,4,3.83,3.83,0,0,0,3.91,4.05A3.39,3.39,0,0,0,45,15l-1.71-.51a1.67,1.67,0,0,1-1.72,1.15,1.85,1.85,0,0,1-1.91-1.73H45s0-.34,0-.63C45.09,10.81,43.69,9.32,41.34,9.32Zm-1.66,3.15A1.63,1.63,0,0,1,41.37,11a1.52,1.52,0,0,1,1.69,1.49Z"/><path id="path18" class="cls-1" d="M50.58,9.35a2.48,2.48,0,0,0-2.18,1.14v-1h-2v7.57h2.05V12.75a1.42,1.42,0,0,1,1.41-1.57,1.34,1.34,0,0,1,1.4,1.52v4.42h2V12.35C53.32,10.68,52.46,9.35,50.58,9.35Z"/><path id="path20" class="cls-1" d="M58.2,15.47a1.94,1.94,0,0,1-1.94-2.14,1.92,1.92,0,0,1,1.91-2.12,1.64,1.64,0,0,1,1.67,1.27l1.83-.61a3.45,3.45,0,0,0-3.55-2.55,3.83,3.83,0,0,0-2.82,1.16,3.88,3.88,0,0,0-1.1,2.85,3.9,3.9,0,0,0,4,4,3.5,3.5,0,0,0,3.53-2.55l-1.8-.6A1.72,1.72,0,0,1,58.2,15.47Z"/><path id="path22" class="cls-1" d="M67,9.35a2.72,2.72,0,0,0-2.07.83V6h-2V17.12h2V12.67a1.43,1.43,0,0,1,1.42-1.48,1.34,1.34,0,0,1,1.4,1.53v4.41h2V12.35A2.67,2.67,0,0,0,67,9.35Z"/><path id="path24" class="cls-1" d="M75.82,11.05l-1.63-.35a1.58,1.58,0,0,1-1.4-1.59A1.93,1.93,0,0,1,74.9,7.3a2.21,2.21,0,0,1,2.3,1.86l1.36-.48a3.73,3.73,0,0,0-7.29.54,3,3,0,0,0,2.67,3l1.55.33c1.09.24,1.63.91,1.63,1.7,0,.95-.74,1.74-2.25,1.74a2.51,2.51,0,0,1-1.79-.63,2.45,2.45,0,0,1-.82-1.71l-1.45.46a3.79,3.79,0,0,0,4.08,3.24c2.32,0,3.76-1.52,3.76-3.22C78.65,12.59,77.63,11.45,75.82,11.05Z"/><path id="path26" class="cls-1" d="M83.25,16A2.33,2.33,0,0,1,81,13.41a2.31,2.31,0,0,1,2.24-2.59,1.93,1.93,0,0,1,2,1.57l1.31-.55a3.22,3.22,0,0,0-3.29-2.35,3.7,3.7,0,0,0-3.72,3.92,3.75,3.75,0,0,0,3.74,3.94A3.41,3.41,0,0,0,86.59,15l-1.28-.55A2,2,0,0,1,83.25,16Z"/><path id="path28" class="cls-1" d="M88.69,5.89a1,1,0,1,0,.75.29A1,1,0,0,0,88.69,5.89Z"/><polygon id="rect30" class="cls-1" points="87.99 9.72 89.42 9.72 89.42 17.11 87.99 17.11 87.99 9.72"/><path id="path32" class="cls-1" d="M14.58,8.87A4.1,4.1,0,0,0,15.8,6H14.31a2.62,2.62,0,0,1-.76,1.87L11.66,9.7l-2-2A2.61,2.61,0,0,1,9,6H7.53A4.09,4.09,0,0,0,8.74,8.87l1.87,1.87L8.74,12.61a4.08,4.08,0,0,0-1.21,2.92v2.29H9.18V16.73h1.66v1.09h1.64V16.73h1.65v1.09H15.8V15.53a4.11,4.11,0,0,0-1.21-2.92l-1.38-1.36-1,1.05,1.37,1.36a2.63,2.63,0,0,1,.77,1.58H9a2.68,2.68,0,0,1,.76-1.58Z"/><path id="path34" class="cls-1" d="M11.61,23.21a11.61,11.61,0,1,1,11.6-11.6A11.6,11.6,0,0,1,11.61,23.21Zm0-21.89A10.29,10.29,0,1,0,21.9,11.61,10.28,10.28,0,0,0,11.61,1.32Z"/></g></g></svg> </span> <span id="bechsci-tooltip-{{$ctrl.productId}}" class="bechsci-tooltip-icon" data-toggle="tooltip" ng-click="$ctrl.showBechSciTooltip()" close-tooltip="$ctrl.closeBechSciTooltip()" data-html="true" title="Clicking this link will redirect you to the <a href='https://www.benchsci.com/' target='_blank'> BenchSci</a> website that provides third-party scientific content. 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src="https://www.thermofisher.com/antibody/images/150/tfs_19211_DDDT-15-803-g0005.jpg?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)"/> </div> </div> </div> </div> </div> <noscript> <div itemscope itemtype="http://schema.org/ImageGallery"> <h2>Product Image Gallery</h2> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/46-1631-82-CD163-antibody-flow-cytometry-062217lsrds-001_20170728195150.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">BALB/c mouse splenocytes were stained with F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-48-4801) and 0.125 µg of Rat IgG2a kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4321) (left) or 0.125 µg of CD163 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-1631) (right). Total cells were used for analysis.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24810_12935_2022_2769_Fig2_HTML.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Fig. 2 ADMA Drives Macrophages Towards an M2-like subtype. RAW264.7 cells were treat-ed with DPBS (control) or ADMA (5 mM) for 3 days. RNA was collected and expression of M1- and M2-related genes was analyzed. Many M2 related markers were strikingly induced, in-cluding ARG ( A ), CD163 ( B ) and CD206 ( C ) while the M1 related markers NOS ( D ) and TNFalpha ( E ) were slightly or not impacted by ADMA. However, some other M2 related markers were downregulated such as IL10 ( F ) and Dectin-1 ( G ). Additionally, the chemotactic receptor CCR2 was also downregulated by ADMA ( H ). The most dramatically upregulated M2 markers (CD163 and CD206) by RNA expression were validated at the protein level by flow cytometry ( I ). Data are presented as mean +- SD. (*p < 0.05, *** p<0.001, ****p<0.0001), N=4 for all groups</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_15351_nihms-1633903-f0013.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Immunohistochemistry (IHC)" title="CD163 Antibody (46-1631-82) in IHC"> <figcaption itemprop="caption">Extended Data Figure 7: Early profibrotic macrophages express M2a markers and are lost upon anti-CSF1 treatment. ( a ) Scatter plot showing individual values and mean +- SEM of the number of F4-80 positive cells per field of view in IgG1 treated (n = 6) or anti-CSF1 treated (n = 4) PyMT tumors from 8-week-old mice. ( b-c ) Scatter plots showing individual values and mean +- SEM of the percentage of F4/80 positive cells expressing markers MHCII and CD86 ( b ) and markers CD163 and Relmalpha ( c ) in IgG1 treated (n = 6) or anti-CSF1 treated (n = 4-5) PyMT tumors from 8-week-old mice. ( d-g ) Scatter plots showing individual values and mean +- SEM of the number of cells per field of view (FOV) of other non-macrophage immune populations CD11b + F4-80 - ( d ), MHCII hi F4-80 - ( e ), CD45 + CD4 + ( f ), and CD45 + CD8 + ( g ) in IgG1 (n = 4 e,g ; n = 5 d,f ) and anti-CSF1 (n = 6) treated mice. Statistical analysis ( a-g ) was performed using two-tailed Mann-Whitney U test ( a ) *p = 0.019 ( b ) MHCII hi p = 0.7190, CD86 + p = 0.4286 ( c ) CD163 + *p = 0.0303, Relmalpha + p = 0.0381 ( d ) p = 0.9719 ( e ) p = 0.1524 ( f ) p = 0.3074 ( g ) p = 1.000. ( h ) Heat map of selected differentially expressed genes from macrophages sorted from 8-week and 11-week PyMT tumors. ( i ) Representative images from 8-week-old IgG1 treated and anti-CSF1 treated PyMT mice stained for cytokeratin 8/18 (green), PDGFRalpha (magenta), vimentin (yellow), and DAPI (blue). Scale bar is 100 mum. ( j ) Scatt</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29209_gr5.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Accumulation of hepatic myeloid cells in tumors of Shp2 DeltaHK mice. ( A ) FACS analysis to evaluate ratios of total macrophages in CD45 cells in the livers. ( B ) FACS analysis to determine the ratios of KCs, non-KCs, MDSCs, and DCs in hepatic CD45 cells (n = 8). ( C ) Representative immunostaining and quantification of TAMs in the liver. Magnification, x100; scale bar : 100 mum. ( D ) FACS analysis to quantify the ratios of Ki67 non-KC macrophages in hepatic CD45 cells. ( E ) Quantitative reverse-transcription PCR to measure mRNA levels of cytokines and other genes as shown. (n = 8 per group) ( F ) FACS analysis to quantify the ratios of CD11b DCs in hepatic CD45 cells. Flow cytometric analysis to show ratios of ( G ) CD163 cells, ( H ) CD206 cells, ( I ) MHCII cells, ( J ) mean fluorescence intensity of CD11b, and ( K ) F4/80 in KCs or non-KC macrophages in the same mouse. Phenotypic comparison between KCs and non-KC macrophages were evaluated by a paired-samples t test. * P < .05, ** P < .01, and *** P < .001. ADM, adrenomedullin; Arg-1, arginase-1; bFGF, basic fibroblast growth factor; FMI, median fluorescence intensity; HGF, hepatocyte growth factor; iNOS, inducible nitric oxide synthase; Mac, macrophage; MMP, matrix metalloproteinase; PGF, placental growth factor; TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29209_gr7.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Figure 7 FACS analysis to show membrane marker expression of KCs and non-KC macrophages. Representative flow cytometric images to show expression of ( A ) CD163, ( B ) CD206, ( C ) MHCII, ( D ) CD11b, and ( E ) F4/80 in KCs and non-KC macrophages in the liver. FSC-A, forward scatter-area.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24811_nihms-1846963-f0002.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Figure 2. Immuno-profiling revealed an overall immune-suppressive TME in Tmem30 -/- (PS out ) tumors (A-G) Flow cytometry quantitative analysis of TILs at the endpoint (day 16). Shown are no infiltrating differences in CD45 + CD11b - CD3 + CD4 + T cells (B), CD45 + CD11b - CD3 + CD8 + T cells (C), CD45 + CD11b - CD3 + CD4 + CD25 + Foxp3 + Tregs (D), and CD45 + CD11b + Ly6G - Ly6C + Mo-MDSC (F); but more infiltrating cells in CD45 + CD11b + Ly6G - Ly6C - F4/80 TAMs (E) and CD45 + CD11b + Ly6C - Ly6G + PMN-MDSC (G). (H) Representative flow plots of MHC II expression in TAMs. (I) Flow cytometry analysis of MHC II + and MHC II - TAM distribution (n = 5). (J and K) Flow cytometry quantitative analysis of MHC II + and MHC II - TAMs (n = 5). (L) RNA-seq analysis of sorted TAMs in WT or PS out ( Tmem30 -/- ) TMEs. Shown is the Z score of gene expression (n = 4 for PS out and n = 3 for WT). (M and N) Geometric mean fluorescence intensity (gMFI) of CD163 (M) and CD206 (N) shows higher expression on TAMs (n = 5). (O-Q) qPCR analysis of MHC II, CD163, and CD206 in MHC II + and MHC II - TAMs from both MC38-WT and Tmem30a -/- TMEs (n = 4). (R and S) gMFI of PD-1 (R) and Tim-3 (S) shows lower expression on CD8 + T cells (n = 5). (T) Flow cytometry analysis of PD-1 and TIM-3 CD8 + T cell distribution (n = 5). (U) Representative flow plots and analysis of tetramer + in CD8 + T cells (n = 5). Data were representative of at least two independent experiments (except RNAseq). Data represent mean</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24809_12964_2022_957_Fig8_HTML.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Fig. 8 AP000439.2-deleted exosome suppressed tumor growth. Xenograft tumor was established by injecting Caki-1 cells, in the meantime, mice treated with AP000439.2-deleted exosome or NC exosome via tail vein injection. A Xenograft tumor images from each mouse. B Body weight curve of mice. C Tumor weight of mice from two groups. D Tumor volume curve of mice from two groups. E The proportion of M1 (F4/80 + NOS2 + ) and M2 (F4/80 + CD163 + ) macrophages in tumor tissues by flow cytometry. *p < 0.05, **p < 0.01. Two-tailed Student's t-test</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24585_gr9.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Figure 9 M2 macrophages are required for normal as well as ON101-enhanced diabetic wound healing. ( a-c ) Subcutaneous injection of m-Clo or m-Enc during normal wound healing in C57BL/6 mice. ( a ) Schematic; n = 4 mice per group. ( b ) Wound recovery rate, calculated as the percentage change from the original size (day 0) and images of wounds at the indicated times. ( c )Biopsies from the point of wounding were analyzed by flow cytometry to detect the proportions of M2 macrophages (F4/80 + /CD163 + ) at indicated time point. Data represent the percentages of F4/80 + or CD163 + macrophages. ( d-h ) M2 macrophage dependence of ON101-promoted diabetic wound healing. m-Clo/m-Enc. ( d ) Schematic; n = 4 mice per group. ( e ) Wound recovery rate (percentage change from day 0) and images of wounds at the indicated times for each treatment group. ( f ) H&E staining represents re-epithelization (dotted line) and the WB in the indicated treatment. ( g ) FACS analysis of the proportions of M2 macrophages (F4/80 + /CD163 + ) in wound surroundings. ( h ) IHC staining of wound biopsies with indicated antibodies on day 9 after wounding. # denotes wound bed. Bar = 250 mum. Data represent means +- SEM. P values in b and e were analyzed using two-way repeated-measures ANOVA, wheares that in g was analyzed using Student's t -test. * P < 0.05, ** P < 0.01, and *** P < 0.001. alpha-SMA, alpha-smooth muscle actin; m-Enc, m-Encapasome; IHC, immunohistochemistry; iNOS, inducible nitrate oxide synth</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19182_WJSC-13-1762-g004.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Figure 4 IF-C2C12-Exos induced M1 macrophage polarization in vitro . A: Images of macrophages cultured with different concentrations of C2C12-Exos for 2 d (1 x 10 9 , 1 x 10 10 , 2 x 10 10 /medium). Scale bar = 200 mum or 100 mum; B: Immunofluorescence localization of CD86 (red), marker of M1 and CD206 (green), marker of M2 after culture with different concentrations of C2C12-Exos for 2 d. Scale bar = 90 mum. Arrows indicate CD206 or CD86 positive cells; C: Representative flow cytometry plots showing the percentages of M1 (CD86 + CD4/80+) and M2 (CD163 + CD4/80+) phenotype in macrophages after culture with different concentrations of C2C12-Exos for 24 h; D: Quantification of flow cytometry data ( n = 3); E: M2 macrophages percentage. Data are presented as mean +- SD. a P < 0.05; b P < 0.01. NS: Not significant.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17688_41434_2021_269_Fig5_HTML.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Fig. 5 Expression of AAT in human MPhi. A Representative AAT western blot analysis of human MPhi supernatant and MPhi lysates. Human serum was used as a positive control. Vinculin band in cellular lysates served as loading control. B ELISA quantification of AAT secretion by human MPhi. n = 3. C Representative May-Grunwald-Giemsa staining of MPhi cytospins. Scale bar = 20 mum. D Representative flow cytometric analysis of myeloid and MPhi-specific surface marker expression by human MPhi. E GM-CSF uptake from cell culture medium by MPhi. A well without cells was used as a negative control. n = 3. All data represent independent cell isolations and transductions.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_13068_ijms-22-03188-g006.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Figure 6 M.C increased the ratio of M2a and M2c macrophages. Spleen-derived cells from the sarcopenia mouse model were assessed by flow cytometry. ( A , B ) Representative flow cytometry (FACS) dot plots of CD206, CD163 in CD11b + F4/80 + , and CD45 + cells. CD206 - CD163 - M1 macrophages; CD206 + CD163 - M2a macrophages; CD206 + CD163 + M2c macrophages; ( C - E ) graphs of M1 macrophage, M2a macrophage, and M2c macrophage, presented as % of CD11b + F4/80 + cells; ( F ) the CD4 + /CD8 ratio of CD45 + cells in splenocytes, presented as the mean +- SEM ( n = 4/group). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CIS+PBS, using a Bonferroni post hoc test after one-way ANOVA.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19211_DDDT-15-803-g0005.jpg?time=20240507?time=20240507" alt="CD163 Antibody in Flow Cytometry (Flow)" title="CD163 Antibody (46-1631-82) in Flow"> <figcaption itemprop="caption">Figure 5 CNT facilitated macrophage polarizing towards M2 phenotype in vitro. ( A ) Flow cytometry analysis and quantification of the percentage of CD163 positive cells. ( B ) qPCR results of the relative expressions of M2 markers, including Arg1, Mrc1, Retnla, and Chi3l3. ( C ) ELISA assays of the levels of IL-6, TNF-alpha and IL-10 in cell supernatants. Data were expressed as mean +- SD. Abbreviations: Con, control; CNT-L, low dose of CNT; CNT-H, high dose of CNT.</figcaption> </figure> </div> </div> </noscript> <div id="tf-related-products-container-new"></div> <div class="product-details"> <div class="container"> <div class="row"> <div class="large-span-12 medium-span-12 small-span-12"> <h2 class="pdp-sub-heading product_details_heading">Product Details</h2></div> <div class="hidden-sku"> <h2>46-1631-82</h2> </div> <div class="product-detail-section tested-app-section new-tbl-design product-detail-section table-spec"> <div class="product-container-head"> <div class="product-item"> Applications </div> <div class="product-item dilution-lbl-head "> Tested Dilution </div> <div class="product-item published-applications"> Publications </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (IHC)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHC-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Flow Cytometry (Flow)</h2></div> <div class="product-item "> <span class="dilution-lbl">0.25 µg/test</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#Flow-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 10 publications</span> <span class="mobile-view">10 publications</span></a> </div> </div> </div> <div class="product-detail-section tested-app-section new-tbl-design product-specific-section table-spec"> <div class="product-container-head"> <div class="product-item">Product Specifications</div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Species Reactivity</h2> </div> <div class="product-item left-flex-cls"> <span title="Mus musculus">Mouse</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Published species</h2> </div> <div class="product-item left-flex-cls"> <span>Not Applicable</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2> Host/Isotype</h2> </div> <div class="product-item left-flex-cls"> <span title="Rattus">Rat / IgG2a, kappa</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Recommended Isotype Control</h2> </div> <div class="product-item left-flex-cls"> <a href="//www.thermofisher.com/antibody/product/46-4321-82"> Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™</a> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Class</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Monoclonal </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Type</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Antibody </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Clone</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> TNKUPJ </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Conjugate</h2> </div> <div class="product-item left-flex-cls"> <a ng-if="$ctrl.checkConjugate('PerCP-eFluor™ 710 ') && '/us/en/home/life-science/cell-analysis/fluorophores/percp-efluor-710.html?cid=bid_pca_aup_r01_co_cp1603_pjt0000_abs00000_0wm_tfs_il_crs_cs_s00_dye'.trim() != ''" href="/us/en/home/life-science/cell-analysis/fluorophores/percp-efluor-710.html?cid=bid_pca_aup_r01_co_cp1603_pjt0000_abs00000_0wm_tfs_il_crs_cs_s00_dye" ng-attr-target="{{$ctrl.getRequestCustomLinkTarget()}}" class="conjugate-value">PerCP-eFluor™ 710 </a> <span ng-if="($ctrl.checkConjugate('PerCP-eFluor™ 710 ') && '/us/en/home/life-science/cell-analysis/fluorophores/percp-efluor-710.html?cid=bid_pca_aup_r01_co_cp1603_pjt0000_abs00000_0wm_tfs_il_crs_cs_s00_dye'.trim() == '')" class="conjugate-value">PerCP-eFluor™ 710 </span> <span ng-if="!$ctrl.checkConjugate('PerCP-eFluor™ 710 ')" class="conjugate-value">PerCP-eFluor™ 710 </span> <div class="dropdown conjugate-dropdown"> <button class="btn-link conjugate-btn" type="button" data-toggle="dropdown"> View additional formats <span class="caret"></span> </button> <ul class="dropdown-menu dropdown-menu-fixed"> <li><a class="conjugate-color-padding" href="/antibody/product/14-1631-82"> Unconjugated <span class="conjugate-indicator" style="background-color:"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/58-1631-82"> Alexa Fluor 532 <span class="conjugate-indicator" style="background-color:#B3FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/17-1631-82"> APC <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/366-1631-82"> Brilliant UV 615 <span class="conjugate-indicator" style="background-color:#FF8900"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/376-1631-82"> Brilliant UV 661 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/367-1631-82"> Brilliant UV 737 <span class="conjugate-indicator" style="background-color:#808080"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/404-1631-82"> Brilliant Violet 421 <span class="conjugate-indicator" style="background-color:#5400FF"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/48-1631-82"> eFluor 450 <span class="conjugate-indicator" style="background-color:#0028FF"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/11-1631-82"> FITC <span class="conjugate-indicator" style="background-color:#1FFF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/12-1631-82"> PE <span class="conjugate-indicator" style="background-color:#F0FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/25-1631-82"> PE-Cyanine7 <span class="conjugate-indicator" style="background-color:#808080"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/61-1631-82"> PE-eFluor 610 <span class="conjugate-indicator" style="background-color:#FF9B00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/62-1631-82"> Super Bright 436 <span class="conjugate-indicator" style="background-color:#3D00FF"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/63-1631-82"> Super Bright 600 <span class="conjugate-indicator" style="background-color:#FFBE00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/67-1631-82"> Super Bright 702 <span class="conjugate-indicator" style="background-color:#8D0000"></span> </a></li> <li class="drop-down-link-ctnr"> <a class="conjugate-dropdown-link" href="/us/en/home/global/forms/antibody-conjugates-custom-form.html" ng-attr-target="$ctrl.getRequestCustomLinkTarget()">Request custom conjugation <svg class="svg-icon external-link-icon" ng-if="$ctrl.showExternalLinkIcon()"> <use href="#external-link"></use> </svg> </a> </li> </ul> </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Excitation/Emission Max</h2> </div> <div class="product-item left-flex-cls"> <span>482/708 nm</span> <a target="_blank" class="spectra-view" href="/order/fluorescence-spectraviewer?SID=srch-svtool&UID=percp_ef710"> <span class="view-spectra"> View spectra</span> <span class="spectra-view-icon "><img src="/order/genome-database/assets/img/spectra.png" alt="spectra"/></span> </a> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Form</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Liquid </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Concentration</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.2 mg/mL </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Purification</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Affinity chromatography </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage buffer</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> PBS, pH 7.2 </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Contains</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.09% sodium azide </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 4° C, store in dark, DO NOT FREEZE! </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Shipping conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Ambient (domestic); Wet ice (international) </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"><h2>RRID <span id="rrid-tooltip" class="rrid-tooltip-icon" ng-include="'/order/genome-database/webapp/assets/core/img/svg/mono_tooltip.svg'" data-toggle="tooltip" ng-click="$ctrl.showRridTooltip()" close-tooltip="$ctrl.closeRridTooltip()" title="Research Resource Identifiers (#RRID) are ID numbers assigned to help researchers cite key resources in the biomedical literature to improve transparency of research methods."> </span></h2> </div> <div class="product-item left-flex-cls">AB_2716956</div> </div> </div> <div class="product-specific-details"> <div class="product-specific-details-tbl product-specific-add-border" > <div class="product-info-para"> <h4 class="product-info-para-heading"> Product Specific Information </h4> <p>Description: This TNKUPJ monoclonal antibody recognizes mouse CD163. CD163 is a 130kDa surface receptor expressed by certain subsets of tissue macrophages, including splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages and a small fraction of peritoneal macrophages. In contrast to human blood monocytes, mouse monocytes do not express CD163. Also, unlike human CD163, mouse CD163 is not as readily induced by M2 polarizing cytokines, and it is not a good marker of M2 macrophages. No common cell lines of monocytic or macrophage origin express mouse CD163. In humans, CD163 has been shown to be proteolytically cleaved and shed from the cell surface, and it acts as a soluble anti-inflammatory factor. <br /><br />This TNKUPJ antibody will detect CD163 on fixed and permeabilized cells allowing for staining of the intracellular pool of this receptor. Although CD163 is relatively stable to collagenase digestion, aggressive tissue dissociation protocols might potentially decrease the amount of surface CD163. In these cases intracellular detection is recommended.<br /><br />Applications Reported: This TNKUPJ antibody has been reported for use in flow cytometric analysis.<br /><br />Applications Tested: This TNKUPJ antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.<br /><br />PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.<br /><br />Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.<br /><br />Fixation: Samples can be stored in IC Fixation Buffer (<a href="https://www.thermofisher.com/order/catalog/product/00-8222-49">Product # 00-8222</a>) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href="https://www.thermofisher.com/order/catalog/product/00-5333-57">Product # 00-5333</a>) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.<br /><br />Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.</p> </div> <div class="product-info-para"> <h4 class="product-info-para-heading"> Target Information </h4> <p class="product-specific-target-info">CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.</p> </div> <p class="product-details-regulatoryStmt">For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.</p> </div> <div class="product-detail-panel-builder"> <tf-promo-ad ng-if="$ctrl.showPromoAd" show-promo-ad="$ctrl.showPromoAd"></tf-promo-ad> <div class="product-detail-video"> <div tf-video-player-new video-id="6206990159001" player-width="368" player-height="210"></div> <div class="panel-builder"> <h4 class="product-info-para-heading"> How to use the Panel Builder </h4> <p>Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.</p> </div> </div> </div> </div> </div> </div> </div> <div id="rootPdpDocLoader"></div> <div id="tf-related-products-container" ng-show="emptyRelatedProductsWidget === false" > <related-products-container ng-if="showRelatedProductsWidget === true" base-url="'/api/magellan/related-products-ui/widget/v2/'" product-type="'antibody'" product-sub-type="'primary'" product-id="'46-1631-82'" country-code="'sg'" iso-language="'en'" > </related-products-container> </div> <div class="tf-pdp-divider" ng-if="emptyRelatedProductsWidget === false" ></div> <div class="references reference-section-1" id="references-component-id"> <div id="references-section-id"> <reference-section ng-if="loadReferences" default-display-card-limit="3" reference-count="11" sub-type="'Antibody'" application-data='{"citationApplications":[{"applicationAbbreviation":"IHC","citations":[{"title":"Tumour-associated macrophages drive stromal cell-dependent collagen crosslinking and stiffening to promote breast cancer aggression.","summary":"Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Immunohistochemistry","authors":"Maller O,Drain AP,Barrett AS,Borgquist S,Ruffell B,Zakharevich I,Pham TT,Gruosso T,Kuasne H,Lakins JN,Acerbi I,Barnes JM,Nemkov T,Chauhan A,Gruenberg J,Nasir A,Bjarnadottir O,Werb Z,Kabos P,Chen YY,Hwang ES,Park M,Coussens LM,Nelson AC,Hansen KC,Weaver VM","literatureId":"33257795","journal":"Nature materials","volume":"20","pageNumber":"548","publishedDate":"Apr 1, 2021, 4:00:00 AM","url":"http://dx.doi.org/10.1038/s41563-020-00849-5","benchSci":true,"imageURL":"https://www.thermofisher.com/antibody/images/250/tfs_15351_nihms-1633903-f0013.jpg?time\u003d20240507","imageId":"938242","journalAbbr":"Nat Mater","sortOrder":7,"species":[],"speciesDilutionMap":{"Not Applicable":"Not Cited"},"abbreviationToNameMap":{"IHC":"Immunohistochemistry"},"newSpeciesDilutionMap":{"~IHC~Not Cited":""}}],"citationsCount":1,"speciesList":[],"journals":["Nat Mater"],"speciesMap":{},"journalsMap":{"Nat Mater":1},"sortedMap":{}},{"applicationAbbreviation":"Flow","citations":[],"citationsCount":10,"speciesList":[],"journals":["World J Stem Cells","Cancer Cell Int","Cell Commun Signal","Int J Mol Sci","Cell Mol Gastroenterol Hepatol","EMBO Rep","Drug Des Devel Ther","JID Innov","Cell Rep","Gene Ther"],"speciesMap":{},"journalsMap":{"World J Stem Cells":1,"Cancer Cell Int":1,"Cell Commun Signal":1,"Int J Mol Sci":1,"Cell Mol Gastroenterol Hepatol":1,"EMBO Rep":1,"Drug Des Devel Ther":1,"JID Innov":1,"Cell Rep":1,"Gene Ther":1},"sortedMap":{}}]}'></reference-section> </div> <noscript> <div itemscope itemtype="https://schema.org/ScholarlyArticle"> <h2>Product References</h2> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36828281"> <div itemprop="name">Shp2 Deficiency in Kupffer Cells and Hepatocytes Aggravates Hepatocarcinogenesis by Recruiting Non-Kupffer Macrophages.</div> </a> <div itemprop="about">Cellular and molecular gastroenterology and hepatology</div> <div itemprop="author">Du L,Ji Y,Xin B,Zhang J,Lu LC,Glass CK,Feng GS</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Mon May 22 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36802267"> <div itemprop="name">Pericyte stem cells induce Ly6G<sup>+</sup> cell accumulation and immunotherapy resistance in pancreatic cancer.</div> </a> <div itemprop="about">EMBO reports</div> <div itemprop="author">Wu Z,Thierry K,Bachy S,Zhang X,Gamradt P,Hernandez-Vargas H,Mikaelian I,Tonon L,Pommier R,Zhao Y,Bertolino P,Hennino A</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Wed Apr 05 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36017415"> <div itemprop="name">Restoring Prohealing/Remodeling-Associated M2a/c Macrophages Using ON101 Accelerates Diabetic Wound Healing.</div> </a> <div itemprop="about">JID innovations : skin science from molecules to population health</div> <div itemprop="author">Lin CW,Chen CC,Huang WY,Chen YY,Chen ST,Chou HW,Hung CM,Chen WJ,Lu CS,Nian SX,Chen SG,Chang HW,Chang VHS,Liu LY,Kuo ML,Chang SC</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Thu Sep 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36376929"> <div itemprop="name">Tumor cell-derived asymmetric dimethylarginine regulates macrophage functions and polarization.</div> </a> <div itemprop="about">Cancer cell international</div> <div itemprop="author">Chen YL,Lowery AT,Lin S,Walker AM,Chen KE</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Tue Nov 15 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36153596"> <div itemprop="name">Exosomal AP000439.2 from clear cell renal cell carcinoma induces M2 macrophage polarization to promote tumor progression through activation of STAT3.</div> </a> <div itemprop="about">Cell communication and signaling : CCS</div> <div itemprop="author">Shen T,Miao S,Zhou Y,Yi X,Xue S,Du B,Tang C,Qu L,Fu D,Jia R,He H</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Sat Sep 24 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36323258"> <div itemprop="name">Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.</div> </a> <div itemprop="about">Cell reports</div> <div itemprop="author">Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Tue Nov 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33257795"> <div itemprop="name">Tumour-associated macrophages drive stromal cell-dependent collagen crosslinking and stiffening to promote breast cancer aggression.</div> </a> <div itemprop="about">Nature materials</div> <div itemprop="author">Maller O,Drain AP,Barrett AS,Borgquist S,Ruffell B,Zakharevich I,Pham TT,Gruosso T,Kuasne H,Lakins JN,Acerbi I,Barnes JM,Nemkov T,Chauhan A,Gruenberg J,Nasir A,Bjarnadottir O,Werb Z,Kabos P,Chen YY,Hwang ES,Park M,Coussens LM,Nelson AC,Hansen KC,Weaver VM</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Immunohistochemistry</div> <div itemprodp="datePublished">Thu Apr 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34909122"> <div itemprop="name">Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation.</div> </a> <div itemprop="about">World journal of stem cells</div> <div itemprop="author">Luo ZW,Sun YY,Lin JR,Qi BJ,Chen JW</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Fri Nov 26 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34276045"> <div itemprop="name">Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo.</div> </a> <div itemprop="about">Gene therapy</div> <div itemprop="author">Janosz E,Hetzel M,Spielmann H,Tumpara S,Rossdam C,Schwabbauer M,Kloos D,von Kaisenberg C,Schambach A,Buettner FFR,Janciauskiene S,Lachmann N,Moritz T</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Wed Sep 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33804803"> <div itemprop="name">Magnoliae Cortex Alleviates Muscle Wasting by Modulating M2 Macrophages in a Cisplatin-Induced Sarcopenia Mouse Model.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Hong M,Han IH,Choi I,Cha N,Kim W,Kim SK,Bae H</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Sat Mar 20 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33654384"> <div itemprop="name">Convallatoxin Promotes M2 Macrophage Polarization to Attenuate Atherosclerosis Through PPARγ-Integrin α<sub>v</sub>β<sub>5</sub> Signaling Pathway.</div> </a> <div itemprop="about">Drug design, development and therapy</div> <div itemprop="author">Zhang Y,Shi X,Han J,Peng W,Fang Z,Zhou Y,Xu X,Lin J,Xiao F,Zhao L,Lin Y</div> <div itemprop="description">Published figure using CD163 monoclonal antibody (Product # 46-1631-82) in Flow Cytometry</div> <div itemprodp="datePublished">Wed Oct 06 00:00:00 EDT 2021</div> </div> </noscript> </div> <div class="border-bottom"></div> <div class="product-bioinformatics-details"> <div class="container"> <h2 class="pdp-sub-heading">Bioinformatics</h2> <div class="row"> <div class="large-span-6 medium-span-12 small-span-12"> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="proteinContent"> <strong>Protein Aliases:</strong> CD163; Scavenger receptor cysteine-rich type 1 protein M130; Soluble sCD163 </p> <p class="view-buttons" id="ptnViewBtn" ng-class="$ctrl.bioContentLines('proteinContent', 'ptnViewBtn')"> <a id="proteinViewMoreBtn" ng-click="$ctrl.bioContentViewMore('proteinViewMoreBtn', 'proteinViewLessBtn', 'proteinContent')"> View more </a> <a id="proteinViewLessBtn" ng-click="$ctrl.bioContentViewLess('proteinViewLessBtn', 'proteinViewMoreBtn', 'proteinContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="geneContent"> <strong>Gene Aliases:</strong> Cd163; CD163v2; CD163v3; M130 </p> <p class="view-buttons" id="geneViewBtn" ng-class="$ctrl.bioContentLines('geneContent', 'geneViewBtn')"> <a id="geneViewMoreBtn" ng-click="$ctrl.bioContentViewMore('geneViewMoreBtn', 'geneViewLessBtn', 'geneContent')"> View more </a> <a id="geneViewLessBtn" ng-click="$ctrl.bioContentViewLess('geneViewLessBtn', 'geneViewMoreBtn', 'geneContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="unitProContent"> <strong>UniProt ID:</strong> <a href="http://www.uniprot.org/uniprot/Q2VLH6" target="_blank">(Mouse) Q2VLH6</a> </p> <p class="view-buttons" id="unitViewBtn" ng-class="$ctrl.bioContentLines('unitProContent', 'unitViewBtn')"> <a id="unitProViewMoreBtn" ng-click="$ctrl.bioContentViewMore('unitProViewMoreBtn', 'unitProViewLessBtn', 'unitProContent')"> View more </a> <a id="unitProViewLessBtn" ng-click="$ctrl.bioContentViewLess('unitProViewLessBtn', 'unitProViewMoreBtn', 'unitProContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="entrezContent"> <strong>Entrez Gene ID:</strong> <a href="http://www.ncbi.nlm.nih.gov/gene?term=93671" target="_blank">(Mouse) 93671</a> </p> <p class="view-buttons" id="etzViewBtn" ng-class="$ctrl.bioContentLines('entrezContent', 'etzViewBtn')"> <a id="entrezViewMoreBtn" ng-click="$ctrl.bioContentViewMore('entrezViewMoreBtn', 'entrezViewLessBtn', 'entrezContent')"> View more </a> <a id="entrezViewLessBtn" ng-click="$ctrl.bioContentViewLess('entrezViewLessBtn', 'entrezViewMoreBtn', 'entrezContent')"> View less </a> </p> </div> </div> <div class="large-span-6 medium-span-12 small-span-12"> <div class="accordion" id="accordion-bioinformatics"> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseOne"> Function(s) </a> </div> <div id="collapseOne" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/scavenger receptor activity"> scavenger receptor activity </a> <a class="icon" href="/antibody/primary/panther/serine protease"> serine protease </a> </div> </div> </div> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseTwo"> Process(es) </a> </div> <div id="collapseTwo" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/receptor-mediated endocytosis"> receptor-mediated endocytosis </a> <a class="icon" href="/antibody/primary/panther/acute-phase response"> acute-phase response </a> <a class="icon" href="/antibody/primary/panther/inflammatory response"> inflammatory response </a> </div> </div> </div> </div> </div> </div> </div> </div> <div class="recommended-products border-bottom hidden">  It has to be done as per old AB suggested Products section. </div> <script type="text/javascript" src="/order/genome-database/assets/core/js/magellanCore.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript"> $m.addPerformanceMark('abPDPScripts:loadBegin'); </script> <script type="text/javascript" src="/order/genome-database/assets/js/shared-module/dist/angular108-document-scripts.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript" src="/order/genome-database/assets/js/shared-module/dist/pdp-combined-scripts.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript" src="/order/genome-database/assets/js/shared-module/dist/combined-vendor.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script defer type="text/javascript" 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CD163 is a 130kDa surface receptor expressed by certain subsets of tissue macrophages, including splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages and a small fraction of peritoneal macrophages. In contrast to human blood monocytes, mouse monocytes do not express CD163. Also, unlike human CD163, mouse CD163 is not as readily induced by M2 polarizing cytokines, and it is not a good marker of M2 macrophages. No common cell lines of monocytic or macrophage origin express mouse CD163. In humans, CD163 has been shown to be proteolytically cleaved and shed from the cell surface, and it acts as a soluble anti-inflammatory factor. <br /><br />This TNKUPJ antibody will detect CD163 on fixed and permeabilized cells allowing for staining of the intracellular pool of this receptor. Although CD163 is relatively stable to collagenase digestion, aggressive tissue dissociation protocols might potentially decrease the amount of surface CD163. In these cases intracellular detection is recommended.<br /><br />Applications Reported: This TNKUPJ antibody has been reported for use in flow cytometric analysis.<br /><br />Applications Tested: This TNKUPJ antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.<br /><br />PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.<br /><br />Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.<br /><br />Fixation: Samples can be stored in IC Fixation Buffer (<a href="https://www.thermofisher.com/order/catalog/product/00-8222-49">Product # 00-8222</a>) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href="https://www.thermofisher.com/order/catalog/product/00-5333-57">Product # 00-5333</a>) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. 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