Human IL-6 Quantikine QuicKit ELISA QK206: R&D Systems

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class="ds_title">Human IL-6 Quantikine QuicKit ELISA </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: QK206</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 4 Citations </a> </span> <span class="reviews review_stars_5"> <a href="#product-reviews" id="product_reviews" class="jump_to" data-tab="6" data-tabname="product-reviews"> 1 Review </a> </span> <a href="/reviews/QK206" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/human-il-6-quantikine-quickit-elisa_qk206" 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data-sku="QC267"><div class="additional_product_label"><a href="/products/human-il-6-quantikine-quickit-control-group-267_qc267">QC267: Human IL-6 Quantikine QuicKit Control Group 267</a></div><div class="et_atc_results"></div><div class="form-item form-item-catnum-qc267 form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-qc267" name="catnum-QC267" value="" size="5" maxlength="3" /></div></div></div> <div class="ds_atc_pre_buttons"></div> <div class="ds_atc_buttons"> <a href="/quote/instruments/ella-automated-immunoassay-system" class="btn btn-secondary atc_request_quote_simple_plex_assay" style="display:none" rel="nofollow">Request a Quote</a> <a href="/services/bulk-quote-request?catnum=QK206&product=Human IL-6 Quantikine QuicKit ELISA" class="btn btn-secondary atc_hidden bulk_btn" style="display:none;" rel="nofollow" title="Competitive discounts on products purchased 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No. QK206 - Human IL-6 Quantikine QuicKit ELISA" href="https://resources.rndsystems.com/images/datasheets/elisa/IL6_QK206_ELISA_2712.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/elisa/IL6_QK206_ELISA_2712.jpg" class="img-fluid" alt="Human IL-6 ELISA Standard Curve" title="Human IL-6 ELISA Standard Curve"> </a> <div class="search_image_other_count"> <span class="search_more_images">4 Images</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="Human IL-6 ELISA Linearity" data-title="Human IL-6 ELISA Linearity" title="Cat. No. QK206 - Human IL-6 Quantikine QuicKit ELISA" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/elisa/IL6_QK206_ELISA_2713.jpg"> <img src="https://resources.rndsystems.com/images/datasheets/elisa/IL6_QK206_ELISA_2713.jpg" class="img-fluid" loading="lazy" alt="Human IL-6 ELISA Linearity" title="Human IL-6 ELISA Linearity"> </a> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="Human IL-6 QuicKit Spiked Recovery Competitor Comparison" data-title="Human IL-6 QuicKit Spiked Recovery Competitor Comparison" title="Cat. No. QK206 - Human IL-6 Quantikine QuicKit ELISA" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/elisa/qk206_human-il-6-quantikine-quickit-elisa-elisa-29202016514.png"> <img src="https://resources.rndsystems.com/images/datasheets/elisa/qk206_human-il-6-quantikine-quickit-elisa-elisa-29202016514.png" class="img-fluid" loading="lazy" alt="Human IL-6 QuicKit Spiked Recovery Competitor Comparison" title="Human IL-6 QuicKit Spiked Recovery Competitor Comparison"> </a> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="Human IL-6 QuicKit Spiked Linearity Competitor Comparison" data-title="Human IL-6 QuicKit Spiked Linearity Competitor Comparison" title="Cat. No. QK206 - Human IL-6 Quantikine QuicKit ELISA" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/elisa/qk206_human-il-6-quantikine-quickit-elisa-elisa-2920201689.png"> <img src="https://resources.rndsystems.com/images/datasheets/elisa/qk206_human-il-6-quantikine-quickit-elisa-elisa-2920201689.png" class="img-fluid" loading="lazy" alt="Human IL-6 QuicKit Spiked Linearity Competitor Comparison" title="Human IL-6 QuicKit Spiked Linearity Competitor Comparison"> </a> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/il-6?category=ELISAs" class="btn btn-secondary">All IL-6 ELISAs</a> </div> </div> <div class="tabbed_content" id="rnd_elisa"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_2" data-tab="2" data-tabname="assay-procedure" id="dsTabs_assay-procedure"> <span>Procedure</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (4)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews (1) </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_sample_activation" id="product-anchor-ds_sample_activation">Sample Values</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_precision" id="product-anchor-ds_precision">Precision</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_recovery" id="product-anchor-ds_recovery">Recovery</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_linearity" id="product-anchor-ds_linearity">Linearity</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <div id="ds_summary" class="datasheet_section"> <h2>Human IL-6 Quantikine QuicKit ELISA Summary</h2> <div class="ds_table summary_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Length</div> <div class="ds_table_cell summary_table_data">80 minutes</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sample Type & Volume Required Per Well</div> <div class="ds_table_cell summary_table_data">Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sensitivity</div> <div class="ds_table_cell summary_table_data">2.95 pg/mL</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Range</div> <div class="ds_table_cell summary_table_data">12.5 - 800 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data">Natural and recombinant human IL-6.</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Cross-reactivity</div> <div class="ds_table_cell summary_table_data">< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Interference</div> <div class="ds_table_cell summary_table_data">No significant interference observed with available related molecules.</div> </div> </div> </div> </div> <div id="ds_sample_activation" class="datasheet_section"> <h2>Sample Values</h2> <b>Serum/Plasma </b>- Ten serum and plasma samples from apparently healthy volunteers were evaluated for the presence of human IL-6 in this assay. All samples measured less than 25 pg/mL. No medical histories were available for the donors used in this study.<div><br></div><div><b>Cell Culture Supernates </b>- Human peripheral blood mononuclear cells (PBMCs) (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were left unstimulated or stimulated with 1.0 μg/mL LPS for 24 hours. Aliquots of the culture supernates were removed, aassayed for levels of human IL-6, and were not detectable or measured 6356 pg/mL, respectively.</div><div><br></div><div>Monocytes were prepared from human PBMCs by adherence to plastic. Unattached cells were removed and attached monocytes were cultured in RPMI supplemented with 10% fetal bovine serum. Cells were left unstimulated or stimulated with 500 ng/mL LPS for 24 hours. Aliquots of the cell culture supernates were removed, assayed for levels of human IL-6, and were not detectable or measured 15,467 pg/mL, respectively.</div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="QK206"></div> <div class="datasheet_section"> <h2>Product Summary</h2> <div> <div>The Quantikine<sup>®</sup> QuicKit™ Human IL-6 Immunoassay is a one step, 80-minute solid phase <span>ELISA designed to measure human IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-6 and antibodies raised against the recombinant protein. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6.</div></span> </div> </div> <div id="ds_precision" class="datasheet_section"> <h2>Precision</h2> <div><strong>Intra-Assay Precision (Precision within an assay)</strong> Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision</div><div><strong>Inter-Assay Precision (Precision between assays)</strong> Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians</div> <p class="precision_title"><strong>Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma</strong></p> <table class="ds_table_html precision_table"> <thead> <tr> <th class="p1"></th> <th colspan="2">Intra-Assay Precision</th> <th colspan="2">Inter-Assay Precision</th> </tr> </thead> <tbody> <tr> <td><strong>Sample</strong></td> <td>1</td> <td>2</td> <td>1</td> <td>2</td> </tr> <tr> <td><strong>n</strong></td> <td>20</td> <td>20</td> <td>10</td> <td>10</td> </tr> <tr> <td><strong>Mean (pg/mL)</strong></td> <td>91.9</td> <td>497</td> <td>87.9</td> <td>487</td> </tr> <tr> <td><strong>Standard Deviation</strong></td> <td>2.15</td> <td>14.3</td> <td>7.49</td> <td>38.6</td> </tr> <tr> <td><strong>CV%</strong></td> <td>2.3</td> <td>2.9</td> <td>8.5</td> <td>7.9</td> </tr> </tbody> </table><br/> </div> <div id="ds_recovery" class="datasheet_section"> <h2>Recovery</h2> <div> <p>The recovery of human IL-6 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated. <p> </div> <table class="ds_table_html recovery_table"> <thead> <th>Sample Type</th> <th>Average % Recovery</th> <th>Range %</th> </thead> <tr> <td>Cell Culture Media (n=4)</td> <td>119</td> <td>114-126</td> </tr> <tr> <td>EDTA Plasma (n=2)</td> <td>117</td> <td>112-122</td> </tr> <tr> <td>Heparin Plasma (n=2)</td> <td>111</td> <td>107-114</td> </tr> <tr> <td>Serum (n=2)</td> <td>106</td> <td>96-115</td> </tr> </table> </div> <div id="ds_linearity" class="datasheet_section"> <h2>Linearity</h2> <div>To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-6 in various matrices and diluted to produce samples with values within the dynamic range of the assay. </div> <div> <img class=" hideoverflow quantikineLinearityImage" alt="Human IL-6 ELISA Linearity" src="https://resources.rndsystems.com/images/datasheets/elisa/IL6_QK206_ELISA_2713.jpg"> </div> <div class="marginBottom"></div> </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> ELISA </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="Human IL-6 ELISA Standard Curve" data-footer="Human IL-6 ELISA Standard Curve" title="Cat. No. - Human IL-6 ELISA Standard Curve" href="https://resources.rndsystems.com/images/datasheets/elisa/IL6_QK206_ELISA_2712.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/elisa/IL6_QK206_ELISA_2712.jpg" class="img-fluid" alt="Human IL-6 ELISA Standard Curve" title="Human IL-6 ELISA Standard Curve"> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Human IL-6 ELISA Standard Curve</span> </p> </div> </div> <div class="row data_ex_row"> <a id="ds_image_1" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> ELISA </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-1" data-title="Human IL-6 QuicKit Spiked Recovery Competitor Comparison" data-footer="" title="Cat. No. - " href="https://resources.rndsystems.com/images/datasheets/elisa/qk206_human-il-6-quantikine-quickit-elisa-elisa-29202016514.png"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/elisa/qk206_human-il-6-quantikine-quickit-elisa-elisa-29202016514.png" class="img-fluid" alt="" title=""> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Human IL-6 QuicKit Spiked Recovery Competitor Comparison</span> IL-6 is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 109% compared to 68% for the top competitor. Plasma recovery is 113% compared to 77% for the top competitor. </p> </div> </div> <div class="row data_ex_row"> <a id="ds_image_2" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> ELISA </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-2" data-title="Human IL-6 QuicKit Spiked Linearity Competitor Comparison" data-footer="" title="Cat. No. - " href="https://resources.rndsystems.com/images/datasheets/elisa/qk206_human-il-6-quantikine-quickit-elisa-elisa-2920201689.png"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/elisa/qk206_human-il-6-quantikine-quickit-elisa-elisa-2920201689.png" class="img-fluid" alt="" title=""> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Human IL-6 QuicKit Spiked Linearity Competitor Comparison</span> IL-6 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 86%-107% compared to 111%-149% for the top competitor. </p> </div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/qk206.pdf?v=20241122" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-il-6-quantikine-quickit-elisa_qk206">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//resources.rndsystems.com/pdfs/msds/qk206.pdf?v=20241122">SDS</a> </div> </div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Storage</div> <div class="ds_table_cell prep_table_data">Store the unopened product at 2 - 8 °C. Do not use past expiration date. </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: IL-6</h2> <p>Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.<p>IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor. </p><p>IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis. </p></p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name:</div> <div class="ds_table_cell background_table_data">Interleukin 6</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs:</div> <div class="ds_table_cell background_table_data">3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names:</div> <div class="ds_table_cell background_table_data">B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A</div> </div> </div> </div> </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/adipocytokines">Adipocytokines</a> </li> <li> <a href="/research-area/cancer-biomarkers">Cancer Biomarkers</a> </li> <li> <a href="/research-area/conventional-classical-dendritic-cells">Conventional/Classical Dendritic Cells</a> </li> <li> <a href="/research-area/cytokines-and-receptors-in-allergy-and-asthma">Cytokines and Receptors in Allergy and Asthma</a> </li> <li> <a href="/research-area/cytokines-and-receptors-in-angiogenesis">Cytokines and Receptors in Angiogenesis</a> </li> <li> <a href="/research-area/cytokines-and-receptors-in-tfh-cell-differentiation-and-function">Cytokines and Receptors in Tfh Cell Differentiation and Function</a> </li> <li> <a href="/research-area/diabetes">Diabetes</a> </li> <li> <a href="/research-area/gamma-delta-t-cells">gamma delta T Cells</a> </li> <li> <a href="/research-area/glucose-homeostasis">Glucose Homeostasis</a> </li> <li> <a href="/research-area/hif-transcription-factors">HIF Transcription Factors</a> </li> <li> <a href="/research-area/il--6-family">IL-6 Family</a> </li> <li> <a href="/research-area/infectious-diseases">Infectious Diseases</a> </li> <li> <a href="/research-area/inflammatory-mediators">Inflammatory Mediators</a> </li> <li> <a href="/research-area/macrophage-activation-markers">Macrophage Activation Markers</a> </li> <li> <a href="/research-area/microglia-activation-markers">Microglia Activation Markers</a> </li> <li> <a href="/research-area/molecules-secreted-by-vsmc">Molecules Secreted by VSMC</a> </li> <li> <a href="/research-area/multiple-myeloma">Multiple Myeloma</a> </li> <li> <a href="/research-area/multiple-sclerosis-and-myelin-related-molecules">Multiple Sclerosis and Myelin Related Molecules</a> </li> <li> <a href="/research-area/myeloid--derived-suppressor-cells--mdsc">Myeloid-derived Suppressor Cells (MDSC)</a> </li> <li> <a href="/research-area/neuroinflammatory-factors">Neuroinflammatory Factors</a> </li> <li> <a href="/research-area/obesity">Obesity</a> </li> <li> <a href="/research-area/plasmacytoid-dendritic-cells">Plasmacytoid Dendritic Cells</a> </li> <li> <a href="/research-area/search-cytokine-and-growth-factor-products">Search Cytokine and Growth Factor Products</a> </li> <li> <a href="/research-area/t-cell-cytokine-signaling">T Cell Cytokine Signaling</a> </li> <li> <a href="/research-area/th17-cells">Th17 Cells</a> </li> <li> <a href="/research-area/th22-cells">Th22 Cells</a> </li> <li> <a href="/research-area/th9-cells">Th9 Cells</a> </li> </ul> </div> </div> <div class="tab_content tab_2" data-tab="2" data-tabname="product-assay-procedure" id="product-assay-procedure"> <a id="assay-procedure"></a> <div id="boxes">  <h2>Assay Procedure</h2> <div id="boxes"> <p>These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.</p> <p><img alt="QuicKit ELISA assay procedure" src="//resources.rndsystems.com/images/site/elisa-assay-principle-1282x215.jpg?v=2" style="display: block;text-align:center;width:100%;" /></p> </div> </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for Human IL-6 Quantikine QuicKit ELISA</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">4</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">4</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Human"> Human </option> </select> </div> <input type="hidden" id="selAntibody" value="All"> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="Cell Culture Supernates"> Cell Culture Supernates </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="4" data-max-show="10"> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/39191755"> The TGF? type I receptor kinase inhibitor vactosertib in combination with pomalidomide in relapsed/refractory multiple myeloma: a phase 1b trial </a> </span><br/> Authors: Malek, E;Rana, PS;Swamydas, M;Daunov, M;Miyagi, M;Murphy, E;Ignatz-Hoover, JJ;Metheny, L;Kim, SJ;Driscoll, JJ;<br/> Nature communications<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/37975220"> Unravelling heterogeneous effects of cancer?associated fibroblasts on poor prognosis markers in breast cancer EM?G3 cell line: In vitro?targeted treatment (anti?IL-6, anti?VEGF-A, anti?MFGE8) based on transcriptomic profiling </a> </span><br/> Authors: Urban, L;Novák, ;?oma, M;Dvo?ánková, B;Lacina, L;áchová, J;Hradilová, M;Svato?ová, P;Kolá?, M;Strnad, H;B?ezinová, J;Smetana, K;Gál, P;Szabo, P;<br/> Oncology reports<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/36118415"> LINC01094/SPI1/CCL7 Axis Promotes Macrophage Accumulation in Lung Adenocarcinoma and Tumor Cell Dissemination </a> </span><br/> Authors: Z Wu, X Bai, Z Lu, S Liu, H Jiang<br/> Journal of Immunology Research, 2022-09-09;2022(0):6450721.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/35068055"> 4-octyl Itaconateï¿½inhibits lipopolysaccharide (LPS)-induced osteoarthritis via activating Nrf2 signalling pathway </a> </span><br/> Authors: Q Zhang, X Bai, R Wang, H Zhao, L Wang, J Liu, M Li, Z Chen, Z Wang, L Li, D Wang<br/> Journal of Cellular and Molecular Medicine, 2022-01-23;0(0):.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> </ol> </div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/elisas"> View all ELISA FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="qk206" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Human IL-6 Quantikine QuicKit ELISA</h3> <div class="row"> <div class="col-xs-12"> <div class="datasheet_section"> <div class="row"> <div class="col-md-6"> <p> Average Rating: 5 <span class="review_stars_5"></span> (Based on 1 Review) </p> <div class="review_rating_progress"> <div class="row"> <div class="col-xs-3"> 5 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="100" 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class="review_filter_rating"> <option value="all">All Ratings</option> <option value="5">5</option> </select> </div> <div class="select_wrapper"> <select class="review_filter_application"> <option value="all">All Applications</option> </select> </div> </div> </div> </div> <br/> <div class="review_row" data-rating="5" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_5">Human IL-6 Quantikine QuicKit ELISA</div> <div class="review_date">By <span class="review_reviewer">James AHODANTIN</span> on 01/08/2022 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Human cell conditioned medium </span> </div> </div> <div class="review_how_used"><p>Very good and sensitive kit. I used 1/1000 diluted conditioned media from macrophages stimulated with LPS. Very reliable kit.</p></div> </div> <div class="col-xs-12 col-sm-2"> <a class="ds-image" data-toggle="lightbox" href="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_qk206_70211_0.jpg" data-title="Human IL-6 Quantikine QuicKit ELISA QK206" data-gallery="Review-images"> <img loading="lazy" class="img-fluid review_image" alt="Human IL-6 Quantikine QuicKit ELISA QK206" title="Human IL-6 Quantikine QuicKit ELISA QK206" src="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_qk206_70211_0.jpg"/> </a> </div> </div> <hr/> </div> </div> </div> </div> <div id="sidebar" class="col-xs-12 col-sm-4 col-md-3 col-lg-3 col-xl-3"> <div class="distributor_region"></div> <div class="widget"> <div class="widget_title">Related Products</div> <div class="widget_content" id="related_products_area"> <ul class="ds_details"> <li> <a href="/target/il-6"> All Products for IL-6 </a> </li> <li> <a href="/target/il-6?category=Primary%20Antibodies"> IL-6 Primary Antibodies </a> 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Human IL-6 Quantikine QuicKit ELISA QK206: R&D Systems