CINXE.COM

<!DOCTYPE html> <html lang="en" dir="ltr"> <head>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-P63WKM1TM1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-P63WKM1TM1'); </script>  <script type="text/javascript" > (function(m,e,t,r,i,k,a){m[i]=m[i]||function(){(m[i].a=m[i].a||[]).push(arguments)}; m[i].l=1*new Date(); for (var j = 0; j < document.scripts.length; j++) {if (document.scripts[j].src === r) { return; }} k=e.createElement(t),a=e.getElementsByTagName(t)[0],k.async=1,k.src=r,a.parentNode.insertBefore(k,a)}) (window, document, "script", "https://mc.yandex.ru/metrika/tag.js", "ym"); ym(55165297, "init", { clickmap:false, trackLinks:true, accurateTrackBounce:true, webvisor:false }); </script> <noscript><div><img src="https://mc.yandex.ru/watch/55165297" style="position:absolute; left:-9999px;" alt="" /></div></noscript>    <title>Search results for: protein fractions</title> <meta name="description" content="Search results for: protein fractions"> <meta name="keywords" content="protein fractions"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="protein fractions" name="q" aria-label="Search"> <button class="btn btn-light my-2 my-sm-0" type="submit"><i class="fas fa-search"></i></button> </form> </div> <div class="collapse navbar-collapse mt-1" id="navbarMenu"> <ul class="navbar-nav ml-auto align-items-center" id="mainNavMenu"> <li class="nav-item"> <a class="nav-link" href="https://waset.org/conferences" title="Conferences in 2024/2025/2026">Conferences</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/disciplines" title="Disciplines">Disciplines</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/committees" rel="nofollow">Committees</a> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownPublications" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Publications </a> <div class="dropdown-menu" aria-labelledby="navbarDropdownPublications"> <a class="dropdown-item" href="https://publications.waset.org/abstracts">Abstracts</a> <a class="dropdown-item" href="https://publications.waset.org">Periodicals</a> <a class="dropdown-item" href="https://publications.waset.org/archive">Archive</a> </div> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/page/support" title="Support">Support</a> </li> </ul> </div> </div> </nav> </div> </header> <main> <div class="container mt-4"> <div class="row"> <div class="col-md-9 mx-auto"> <form method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="protein fractions"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2762</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: protein fractions</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2702</span> Inheritance of Protein Content and Grain Yield in Half Diallel Maize (Zea mays L.) Populations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=G%C3%BCl%20Ebru%20Orhun">Gül Ebru Orhun</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A half diallel crossing design was carried out during 2011 and 2012 growing seasons under Çanakkale-Turkey ecological conditions. In this research, 20 F1 maize hybrids obtained by 6x6 half diallel crossing were used. Gene action for protein content and grain yield traits were explored in half set involving six elite inbred lines. According to the results diallel analysis dominance and additive gene variances were determined for protein content. Variance/Co-variance graphs revealed for grain yield and protein content traits. In this study, inheritance of grain yield and protein content demonstrated over-dominance type of gene action. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=protein" title="protein">protein</a>, <a href="https://publications.waset.org/abstracts/search?q=maize" title=" maize"> maize</a>, <a href="https://publications.waset.org/abstracts/search?q=inheritance" title=" inheritance"> inheritance</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20action" title=" gene action"> gene action</a> </p> <a href="https://publications.waset.org/abstracts/17608/inheritance-of-protein-content-and-grain-yield-in-half-diallel-maize-zea-mays-l-populations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17608.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">525</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2701</span> Interaction of Dietary Protein and Vitamin E Supplementation on Gastrointestinal Nematode (Gnt) Parasitism of Naturally Infected Lambs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ayobami%20Adeyemo">Ayobami Adeyemo</a>, <a href="https://publications.waset.org/abstracts/search?q=Michael%20%20Chimonyo"> Michael Chimonyo</a>, <a href="https://publications.waset.org/abstracts/search?q=Munyaradzi%20Marufu"> Munyaradzi Marufu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gastrointestinal nematode (GNT) infection significantly hinder sustainable and profitable sheep production on rangelands. While vitamin E and protein supplementation have individually proven to improve host immunity to parasitism in lambs, to our knowledge, there is no information on the interaction of dietary vitamin E and protein supplementation on lamb growth and GIN faecal egg counts in naturally infected lambs. Therefore, the current study investigated the interaction of dietary protein and vitamin E supplementation on faecal egg counts (FEC) and growth performance of lambs. Twenty four Dohne Merino lambs aged 12 months were allocated equally to each of four treatment combinations, with six lambs in each treatment group for a period of eight weeks. Treatment one lambs received dietary protein and vitamin E (PE), treatment two lambs received dietary protein and no vitamin E (PNE), treatment three received dietary vitamin E and no protein (NPE), and treatment four received no dietary protein and vitamin E supplementation (NPNE). The lambs were allowed to graze on Pennisetum clandestinum contaminated with a heavy load of nematodes. Dietary protein supplementation increased (P < 0.01) average daily gain (ADG) and body condition scores (BCS). Dietary vitamin E supplementation had no effect (P > 0.05) on ADG and BCS. There was no interaction (P > 0.05) between dietary protein and vitamin E supplementation on ADG and BCS. Combined supplementation of dietary protein and vitamin E supplementation significantly reduced (P < 0.01) faecal egg counts and larval counts, respectively. Also, dietary protein and vitamin E supplementation reduced GNT faecal egg counts over the exposure period. The current findings support the hypothesis that the interaction of dietary protein and vitamin E supplementation reduced faecal egg counts and larval counts in lambs. This necessitates future findings on the interaction of dietary protein and vitamin E supplementation on blood associated profiles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gastrointestinal%20nematodes" title="gastrointestinal nematodes">gastrointestinal nematodes</a>, <a href="https://publications.waset.org/abstracts/search?q=nematode%20eggs" title=" nematode eggs"> nematode eggs</a>, <a href="https://publications.waset.org/abstracts/search?q=Haemonchus" title=" Haemonchus"> Haemonchus</a>, <a href="https://publications.waset.org/abstracts/search?q=Trichostrongylus" title=" Trichostrongylus"> Trichostrongylus</a> </p> <a href="https://publications.waset.org/abstracts/88994/interaction-of-dietary-protein-and-vitamin-e-supplementation-on-gastrointestinal-nematode-gnt-parasitism-of-naturally-infected-lambs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88994.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">209</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2700</span> Effects of Dietary Protein and Lipid Levels on Growth and Body Composition of Juvenile Fancy Carp, Cyprinus carpio var. Koi</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jin%20Choi">Jin Choi</a>, <a href="https://publications.waset.org/abstracts/search?q=Zahra%20Aminikhoei"> Zahra Aminikhoei</a>, <a href="https://publications.waset.org/abstracts/search?q=Yi-Oh%20Kim"> Yi-Oh Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Sang-Min%20Lee"> Sang-Min Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A 4 × 2 factorial experiment was conducted to determine the optimum dietary protein and lipid levels for juvenile fancy carp, Cyprinus carpio var. koi. Eight experimental diets were formulated to contain four protein levels (200, 300, 400, and 500 g kg-1) with two lipid levels (70 and 140 g kg-1). Triplicate groups of fish (initial weight, 12.1±0.2 g fish-1) were hand-fed the diets to apparent satiation for 8 weeks. Weight gain, daily feed intake, feed efficiency ratio and protein efficiency ratio were significantly (P < 0.0001) affected by dietary protein level, but not by dietary lipid level (P > 0.05). Weight gain and feed efficiency ratio tended to increase as dietary protein level increased up to 400 and 500 g kg-1, respectively. Daily feed intake of fish decreased with increasing dietary protein level and that of fish fed diet contained 500 g kg-1 protein was significantly lower than other fish groups. The protein efficiency ratio of fish fed 400 and 500 g kg-1 protein was lower than that of fish fed 200 and 300 g kg-1 protein. Moisture, crude protein and crude lipid contents of muscle and liver were significantly affected by dietary protein, but not by dietary lipid level (P > 0.05). The increase in dietary lipid level resulted in an increase in linoleic acid in liver and muscle paralleled with a decrease in n-3 highly unsaturated fatty acids content in muscle of fish. In considering these results, it was concluded that the diet containing 400 g kg-1 protein with 70 g kg-1 lipid level is optimal for growth and efficient feed utilization of juvenile fancy carp. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fancy%20carp" title="fancy carp">fancy carp</a>, <a href="https://publications.waset.org/abstracts/search?q=dietary%20protein" title=" dietary protein"> dietary protein</a>, <a href="https://publications.waset.org/abstracts/search?q=dietary%20lipid" title=" dietary lipid"> dietary lipid</a>, <a href="https://publications.waset.org/abstracts/search?q=Cyprinus%20carpio" title=" Cyprinus carpio"> Cyprinus carpio</a>, <a href="https://publications.waset.org/abstracts/search?q=fatty%20acid" title=" fatty acid"> fatty acid</a> </p> <a href="https://publications.waset.org/abstracts/17701/effects-of-dietary-protein-and-lipid-levels-on-growth-and-body-composition-of-juvenile-fancy-carp-cyprinus-carpio-var-koi" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17701.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">403</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2699</span> A Principal-Agent Model for Sharing Mechanism in Integrated Project Delivery Context</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shan%20Li">Shan Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Qiuwen%20Ma"> Qiuwen Ma</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Integrated project delivery (IPD) is a project delivery method distinguished by a shared risk/rewards mechanism and multiparty agreement. IPD has drawn increasingly attention from construction industry because of its efficiency of solving adversarial problems and reliability to deliver high-performing buildings. However, some evidence showed that some project participants obtained less profit from IPD projects than the typical projects. They attributed it to the unfair IPD sharing mechanism, which resulted in additional time and cost of negotiation on the sharing fractions among project participants. The study is aimed to investigate the reward distribution by constructing a principal-agent model. Based on cooperative game theory, it is examined how to distribute the shared project rewards between client and non-client parties, and identify the sharing fractions among non-client parties. It is found that at least half of the project savings should be allocated to the non-client parties to motivate them to create more project value. Second, the client should raise his sharing fractions when the integration among project participants is efficient. In addition, the client should allocate higher sharing fractions to the non-client party who is more able. This study can help the IPD project participants make fair and motivated sharing mechanisms. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cooperative%20game%20theory" title="cooperative game theory">cooperative game theory</a>, <a href="https://publications.waset.org/abstracts/search?q=IPD" title=" IPD"> IPD</a>, <a href="https://publications.waset.org/abstracts/search?q=principal%20agent%20model" title=" principal agent model"> principal agent model</a>, <a href="https://publications.waset.org/abstracts/search?q=sharing%20mechanism" title=" sharing mechanism"> sharing mechanism</a> </p> <a href="https://publications.waset.org/abstracts/92285/a-principal-agent-model-for-sharing-mechanism-in-integrated-project-delivery-context" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/92285.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">292</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2698</span> The Determination of Aflatoxins in Paddy and Milled Fractions of Rice in Guyana: Preliminary Results</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Donna%20M.%20Morrison">Donna M. Morrison</a>, <a href="https://publications.waset.org/abstracts/search?q=Lambert%20Chester"> Lambert Chester</a>, <a href="https://publications.waset.org/abstracts/search?q=Coretta%20A.%20N.%20Samuels"> Coretta A. N. Samuels</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20R.%20Ledoux"> David R. Ledoux</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A survey was conducted in the five rice-growing regions in Guyana to determine the presence of aflatoxins in multiple fractions of rice in June/October 2015 growing season. The fractions were paddy, steamed paddy, cargo rice, white rice and parboiled rice. Samples were analyzed by High Performance Liquid Chromatography. A subset of the samples was further analyzed by enzyme-linked immunosorbent assay (ELISA) for concurrence. All analyses were conducted at the University of Missouri, USA. Of the 186 samples tested, 16 had aflatoxin concentrations greater than 20 ppb the recommended limit for aflatoxins in food according to the United States Food and Drug Administration. An additional three samples had aflatoxin B<sub>1</sub> concentrations greater than the European Union Commission maximum levels for aflatoxin B<sub>1</sub> in rice at 5 µg/kg and total aflatoxins (B<sub>1</sub>, B<sub>2</sub>, G<sub>1</sub> and G<sub>2</sub>) at 10 µg/kg. The survey indicates that there is no widespread aflatoxin problem in rice in Guyana. The incidence of aflatoxins appears to be localized. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aflatoxin" title="aflatoxin">aflatoxin</a>, <a href="https://publications.waset.org/abstracts/search?q=enzyme-linked%20immunosorbent%20assay%20%28ELISA%29" title=" enzyme-linked immunosorbent assay (ELISA)"> enzyme-linked immunosorbent assay (ELISA)</a>, <a href="https://publications.waset.org/abstracts/search?q=high-performance%20liquid%20chromatography%20%28HPLC%29" title=" high-performance liquid chromatography (HPLC)"> high-performance liquid chromatography (HPLC)</a>, <a href="https://publications.waset.org/abstracts/search?q=rice%20fractions" title=" rice fractions"> rice fractions</a> </p> <a href="https://publications.waset.org/abstracts/58517/the-determination-of-aflatoxins-in-paddy-and-milled-fractions-of-rice-in-guyana-preliminary-results" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58517.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">265</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2697</span> Isolation, Preparation and Biological Properties of Soybean-Flaxseed Protein Co-Precipitates</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20H.%20Alu%E2%80%99datt">Muhammad H. Alu’datt</a>, <a href="https://publications.waset.org/abstracts/search?q=Inteaz%20Alli"> Inteaz Alli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study was conducted to prepare and evaluate the biological properties of protein co-precipitates from flaxseed and soybean. Protein was prepared by NaOH extraction through the mixing of soybean flour (Sf) and flaxseed flour (Ff) or mixtures of soybean extract (Se) and flaxseed extract (Fe). The protein co-precipitates were precipitated by isoelectric (IEP) and isoelectric-heating (IEPH) co-precipitation techniques. Effects of extraction and co-precipitation techniques on co-precipitate yield were investigated. Native-PAGE, SDS-PAGE were used to study the molecular characterization. Content and antioxidant activity of extracted free and bound phenolic compounds were evaluated for protein co-precipitates. Removal of free and bound phenolic compounds from protein co-precipitates showed little effects on the electrophoretic behavior of the proteins or the protein subunits of protein co-precipitates. Results showed that he highest protein contents and yield were obtained in for Sf-Ff/IEP co-precipitate with values of 53.28 and 25.58% respectively as compared to protein isolates and other co-precipitates. Results revealed that the Sf-Ff/IEP showed a higher content of bound phenolic compounds (53.49% from total phenolic content) as compared to free phenolic compounds (46.51% from total phenolic content). Antioxidant activities of extracted bound phenolic compounds with and without heat treatment from Sf-Ff/IEHP were higher as compared to free phenolic compounds extracted from other protein co-precipitates (29.68 and 22.84%, respectively). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title="antioxidant">antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=phenol" title=" phenol"> phenol</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20co-precipitate" title=" protein co-precipitate"> protein co-precipitate</a>, <a href="https://publications.waset.org/abstracts/search?q=yield" title=" yield"> yield</a> </p> <a href="https://publications.waset.org/abstracts/47994/isolation-preparation-and-biological-properties-of-soybean-flaxseed-protein-co-precipitates" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47994.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">240</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2696</span> Membrane Technologies for Obtaining Bioactive Fractions from Blood Main Protein: An Exploratory Study for Industrial Application</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fatima%20Arrutia">Fatima Arrutia</a>, <a href="https://publications.waset.org/abstracts/search?q=Francisco%20Amador%20Riera"> Francisco Amador Riera</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The meat industry generates large volumes of blood as a result of meat processing. Several industrial procedures have been implemented in order to treat this by-product, but are focused on the production of low-value products, and in many cases, blood is simply discarded as waste. Besides, in addition to economic interests, there is an environmental concern due to bloodborne pathogens and other chemical contaminants found in blood. Consequently, there is a dire need to find extensive uses for blood that can be both applicable to industrial scale and able to yield high value-added products. Blood has been recognized as an important source of protein. The main blood serum protein in mammals is serum albumin. One of the top trends in food market is functional foods. Among them, bioactive peptides can be obtained from protein sources by microbiological fermentation or enzymatic and chemical hydrolysis. Bioactive peptides are short amino acid sequences that can have a positive impact on health when administered. The main drawback for bioactive peptide production is the high cost of the isolation, purification and characterization techniques (such as chromatography and mass spectrometry) that make unaffordable the scale-up. On the other hand, membrane technologies are very suitable to apply to the industry because they offer a very easy scale-up and are low-cost technologies, compared to other traditional separation methods. In this work, the possibility of obtaining bioactive peptide fractions from serum albumin by means of a simple procedure of only 2 steps (hydrolysis and membrane filtration) was evaluated, as an exploratory study for possible industrial application. The methodology used in this work was, firstly, a tryptic hydrolysis of serum albumin in order to release the peptides from the protein. The protein was previously subjected to a thermal treatment in order to enhance the enzyme cleavage and thus the peptide yield. Then, the obtained hydrolysate was filtered through a nanofiltration/ultrafiltration flat rig at three different pH values with two different membrane materials, so as to compare membrane performance. The corresponding permeates were analyzed by liquid chromatography-tandem mass spectrometry technology in order to obtain the peptide sequences present in each permeate. Finally, different concentrations of every permeate were evaluated for their in vitro antihypertensive and antioxidant activities though ACE-inhibition and DPPH radical scavenging tests. The hydrolysis process with the previous thermal treatment allowed achieving a degree of hydrolysis of the 49.66% of the maximum possible. It was found that peptides were best transmitted to the permeate stream at pH values that corresponded to their isoelectric points. Best selectivity between peptide groups was achieved at basic pH values. Differences in peptide content were found between membranes and also between pH values for the same membrane. The antioxidant activity of all permeates was high compared with the control only for the highest dose. However, antihypertensive activity was best for intermediate concentrations, rather than higher or lower doses. Therefore, although differences between them, all permeates were promising regarding antihypertensive and antioxidant properties. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioactive%20peptides" title="bioactive peptides">bioactive peptides</a>, <a href="https://publications.waset.org/abstracts/search?q=bovine%20serum%20albumin" title=" bovine serum albumin"> bovine serum albumin</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrolysis" title=" hydrolysis"> hydrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=membrane%20filtration" title=" membrane filtration"> membrane filtration</a> </p> <a href="https://publications.waset.org/abstracts/60182/membrane-technologies-for-obtaining-bioactive-fractions-from-blood-main-protein-an-exploratory-study-for-industrial-application" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/60182.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">200</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2695</span> Antifungal Protein ~35kDa Produced by Bacillus cereus Inhibits the Growth of Some Molds and Yeasts</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saleh%20H.%20Salmen">Saleh H. Salmen</a>, <a href="https://publications.waset.org/abstracts/search?q=Sulaiman%20Ali%20Alharbi"> Sulaiman Ali Alharbi</a>, <a href="https://publications.waset.org/abstracts/search?q=Hany%20M.%20Yehia"> Hany M. Yehia</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20A.%20Khiyami"> Mohammad A. Khiyami</a>, <a href="https://publications.waset.org/abstracts/search?q=Milton%20Wainwright"> Milton Wainwright</a>, <a href="https://publications.waset.org/abstracts/search?q=Naiyf%20S.%20Alharbi"> Naiyf S. Alharbi</a>, <a href="https://publications.waset.org/abstracts/search?q=Arunachalam%20Chinnathambi"> Arunachalam Chinnathambi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An antifungal protein synthesized by Bacillus cereus has been partially purified by the use of ammonium sulfate precipitation and Sephadex-G-200 column chromatography. The protein was produced from Bacillus cereus grown in potato Dextrose Broth Medium (PDB) at 30 ºC for 3 days at 100 rpm. The protein showed antagonistic effect against some fungi and yeasts. Crude extract from medium and semi-purified protein were tested in vitro against both fungi and yeasts using the disc diffusion method in order to detect the inhibitory effect of the protein. Zones of inhibition of the following diameter were found (mm) were Alternaria alternate (28), Rhodotorula glutinis (20), Fusarium sp. (16), Rhizopus sp. (15), Penicillium digitatum (13), Mucor sp. (13) and Aspergillus niger (10). The isolated protein was found to have a molecular weight of ~35kDa by sodium deodecyl sulfate-poly acrylamide gel electrophoresis. The data showed that the protein of Bacillus cereus has antifungal activity, a fact which points to the possibility of using it as a bio-control agent against some fungi, findings which emphasize the potential role of B. cereus as an important bio-control agent. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacillus%20cereus" title="bacillus cereus">bacillus cereus</a>, <a href="https://publications.waset.org/abstracts/search?q=~35kDa%20protein" title=" ~35kDa protein"> ~35kDa protein</a>, <a href="https://publications.waset.org/abstracts/search?q=molds" title=" molds"> molds</a>, <a href="https://publications.waset.org/abstracts/search?q=yeasts" title=" yeasts"> yeasts</a> </p> <a href="https://publications.waset.org/abstracts/3422/antifungal-protein-35kda-produced-by-bacillus-cereus-inhibits-the-growth-of-some-molds-and-yeasts" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3422.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">291</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2694</span> Evaluation of Ficus racemosa (Moraceae) as a Potential Source for Drug Formulation Against Coccidiosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Naveeda%20Akhtar%20Qureshi">Naveeda Akhtar Qureshi</a>, <a href="https://publications.waset.org/abstracts/search?q=Wajiha">Wajiha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Coccidiosis is a protozoan parasitic disease of genus Eimeria. It is an avian infection causing a great economic loss of 3 billion USD per year globally. A number of anticoccidial drugs are in use however many of them have side effects and cost effective. With increase in poultry demand throughout the world there is a need of more drugs and vaccines against coccidiosis. The present study is based upon the use of F. racemosa a medicinal plant to be a potential source of anticoccidial agents. The methanolic leaves extract was fractionated by column and thin layer chromatography and got nineteen fractions. Each fraction different concentrations was evaluated for its anticoccidial properties in an invitro experiment against E. tenella, E. necatrix and E. mitis. The anticoccidial active fractions were further characterized by spectroscopy (UV-Vis, FTIR) and GC-MS analysis. The in silico molecular docking of active fractions identified compounds were carried out. Among all fractions significantly maximum sporulation inhibition efficacy was shown by F-19 (67.11±2.18) followed by F-15 (65.21±1.34) at concentration of 30mg/ml against E. tenella. The significantly highest sporozoites viability inhibition was shown by F-19 (69.23±2.11) followed by F-15 (67.14±1.52) against E. necatrix at concentration 30mg/ml. Anticoccidial active fractions 15 and 19 showed peak spectrum at 207 and 202nm respectively by UV analysis. Their FTIR analysis confirmed the presence of carboxylic acid, amines, phenols, etc. Anticoccidial active compounds like Cyclododecane methanol, oleic acid, Octadecanoic acid, etc were identified by GC-MS analysis. Identified compounds in silico molecular docking study showed that cyclododecane methanol of F-19 and oleic acid of F-15 showed highest binding affinity with target S-Adenosylmethionine synthase. Hence for further authentication in vivo anticoccidial studies are recommended. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ficus%20racemosa" title="ficus racemosa">ficus racemosa</a>, <a href="https://publications.waset.org/abstracts/search?q=cluster%20fig" title=" cluster fig"> cluster fig</a>, <a href="https://publications.waset.org/abstracts/search?q=column%20chromatography" title=" column chromatography"> column chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=anticoccidial%20fractions" title=" anticoccidial fractions"> anticoccidial fractions</a>, <a href="https://publications.waset.org/abstracts/search?q=GC-MS" title=" GC-MS"> GC-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20docking." title=" molecular docking."> molecular docking.</a>, <a href="https://publications.waset.org/abstracts/search?q=s-adenosylmethionine%20synthase" title=" s-adenosylmethionine synthase"> s-adenosylmethionine synthase</a> </p> <a href="https://publications.waset.org/abstracts/168554/evaluation-of-ficus-racemosa-moraceae-as-a-potential-source-for-drug-formulation-against-coccidiosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/168554.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">85</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2693</span> The Effect of Sorafenibe on Soat1 Protein by Using Molecular Docking Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mahdiyeh%20Gholaminezhad">Mahdiyeh Gholaminezhad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Context: The study focuses on the potential impact of Sorafenib on SOAT1 protein in liver cancer treatment, addressing the need for more effective therapeutic options. Research aim: To explore the effects of Sorafenib on the activity of SOAT1 protein in liver cancer cells. Methodology: Molecular docking was employed to analyze the interaction between Sorafenib and SOAT1 protein. Findings: The study revealed a significant effect of Sorafenib on the stability and activity of SOAT1 protein, suggesting its potential as a treatment for liver cancer. Theoretical importance: This research highlights the molecular mechanism underlying Sorafenib's anti-cancer properties, contributing to the understanding of its therapeutic effects. Data collection: Data on the molecular structure of Sorafenib and SOAT1 protein were obtained from computational simulations and databases. Analysis procedures: Molecular docking simulations were performed to predict the binding interactions between Sorafenib and SOAT1 protein. Question addressed: How does Sorafenib influence the activity of SOAT1 protein and what are the implications for liver cancer treatment? Conclusion: The study demonstrates the potential of Sorafenib as a targeted therapy for liver cancer by affecting the activity of SOAT1 protein. Reviewers' Comments: The study provides valuable insights into the molecular basis of Sorafenib's action on SOAT1 protein, suggesting its therapeutic potential. To enhance the methodology, the authors could consider validating the docking results with experimental data for further validation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=liver%20cancer" title="liver cancer">liver cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=sorafenib" title=" sorafenib"> sorafenib</a>, <a href="https://publications.waset.org/abstracts/search?q=SOAT1" title=" SOAT1"> SOAT1</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20docking" title=" molecular docking"> molecular docking</a> </p> <a href="https://publications.waset.org/abstracts/189263/the-effect-of-sorafenibe-on-soat1-protein-by-using-molecular-docking-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/189263.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">26</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2692</span> Protein and Lipid Extraction from Microalgae with Ultrasound Assisted Osmotic Shock Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nais%20Pinta%20Adetya">Nais Pinta Adetya</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Hadiyanto"> H. Hadiyanto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microalgae has a potential to be utilized as food and natural colorant. The microalgae components consists of three main parts, these are lipid, protein, and carbohydrate. Crucial step in producing lipid and protein from microalgae is extraction. Microalgae has high water level (70-90%), it causes drying process of biomass needs much more energy and also has potential to distract lipid and protein from microalgae. Extraction of lipid from wet biomass is able to take place efficiently with cell disruption of microalgae by osmotic shock method. In this study, osmotic shock method was going to be integrated with ultrasound to maximalize the extraction yield of lipid and protein from wet biomass Spirulina sp. with osmotic shock method assisted ultrasound. This study consisted of two steps, these were osmotic shock process toward wet biomass and ultrasound extraction assisted. NaCl solution was used as osmotic agent, with the variation of concentrations were 10%, 20%, and 30%. Extraction was conducted in 40°C for 20 minutes with frequency of ultrasound wave was 40kHz. The optimal yield of protein (2.7%) and (lipid 38%) were achieved at 20% osmotic agent concentration. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=extraction" title="extraction">extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid" title=" lipid"> lipid</a>, <a href="https://publications.waset.org/abstracts/search?q=osmotic%20shock" title=" osmotic shock"> osmotic shock</a>, <a href="https://publications.waset.org/abstracts/search?q=protein" title=" protein"> protein</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasound" title=" ultrasound"> ultrasound</a> </p> <a href="https://publications.waset.org/abstracts/76886/protein-and-lipid-extraction-from-microalgae-with-ultrasound-assisted-osmotic-shock-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/76886.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">359</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2691</span> Myeloid Zinc Finger 1/Ets-Like Protein-1/Protein Kinase C Alpha Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jer-Yuh%20Liu">Jer-Yuh Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Je-Chiuan%20Ye"> Je-Chiuan Ye</a>, <a href="https://publications.waset.org/abstracts/search?q=Jin-Ming%20Hwang"> Jin-Ming Hwang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Protein kinase C alpha (PKCα) is a key signaling molecule in human cancer development. As a therapeutic strategy, targeting PKCα is difficult because the molecule is ubiquitously expressed in non-malignant cells. PKCα is regulated by the cooperative interaction of the transcription factors myeloid zinc finger 1 (MZF-1) and Ets-like protein-1 (Elk-1) in human cancer cells. By conducting tissue array analysis, herein, we determined the protein expression of MZF-1/Elk-1/PKCα in various cancers. The data show that the expression of MZF-1/Elk-1 is correlated with that of PKCα in hepatocellular carcinoma (HCC), but not in bladder and lung cancers. In addition, the PKCα down-regulation by shRNA Elk-1 was only observed in the HCC SK-Hep-1 cells. Blocking the interaction between MZF-1 and Elk-1 through the transfection of their binding domain MZF-160–72 decreased PKCα expression. This step ultimately depressed the epithelial-mesenchymal transition potential of the HCC cells. These findings could be used to develop an alternative therapeutic strategy for patients with the PKCα-derived HCC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=protein%20kinase%20C%20alpha" title="protein kinase C alpha">protein kinase C alpha</a>, <a href="https://publications.waset.org/abstracts/search?q=myeloid%20zinc%20finger%201" title=" myeloid zinc finger 1"> myeloid zinc finger 1</a>, <a href="https://publications.waset.org/abstracts/search?q=ets-like%20protein-1" title=" ets-like protein-1"> ets-like protein-1</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatocellular%20carcinoma" title=" hepatocellular carcinoma"> hepatocellular carcinoma</a> </p> <a href="https://publications.waset.org/abstracts/78123/myeloid-zinc-finger-1ets-like-protein-1protein-kinase-c-alpha-associated-with-poor-prognosis-in-patients-with-hepatocellular-carcinoma" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/78123.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">227</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2690</span> Profiling, Antibacterial and Antioxidant Activity of Acacia decurrens (Willd) an Invasive South Africa Tree</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Joe%20Modise">Joe Modise</a>, <a href="https://publications.waset.org/abstracts/search?q=Bamidel%20Joseph%20Okoli"> Bamidel Joseph Okoli</a>, <a href="https://publications.waset.org/abstracts/search?q=Nas%20Molefe"> Nas Molefe</a>, <a href="https://publications.waset.org/abstracts/search?q=Imelda%20Ledwaba"> Imelda Ledwaba</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present study describes the chemical profile and antioxidant potential of the stem bark of Acacia decurrens. The methanol fraction of A. decurrens stem bark gave the highest yield (20 %), while the hexane fraction had the lowest yield (0.2 %). The GC-MS spectra of the hexane, chloroform and ethyl acetate fractions confirm the presence of fifty two major compounds and the ICP-OES analysis of the stem bark was found to contain Co(0.41), Zn(1.75), Mn(3.69), Ca(8.67), Ni(10.54), Mg(12.98), Cr(24.38), K(47.88), Fe(154.62) ppm; which is an indication of hyper-accumulation capacity. The UV-Visible spectra of showed four absorption maxima for hexane fraction at 665 (0.028), 410 (0.116), 335 (0.278) and 250 (0.007) nm, three for chloroform fraction at 665 (0.028), 335 (0.278) and 250 (0.007) nm , three for ethyl acetate fraction at 665 (0.070), 390 (0.648) and 345 (0.663) nm and three for methanol fraction at 385 (0.508), 310 (0.886) and 295 (0.899) nm respectively. Quantitative phytochemical screening indicated that the alkaloid (0.6-3.3) % and saponins (5.1-8.6) % contents of the various fractions were significantly lower than the tannin (30.9-55.8) mg TAE/g, steroid(13.92-41.2) %, phenol (40.6-65.5) mgGAE/g and flavonoids (210.2 -284.9) mg RUE/g contents. The antioxidant activity of the fractions was analysed by different methods and revealed good to moderate antioxidant potential with different IC50 values viz. (42.2-49.6) mg/mL for ABTS and (37.8-75.0) μg/ml for DPPH respectively, compared to standard antioxidants. Based on obtained results, the A.decurrens stem bark fractions can be a source of safe, sustainable natural antioxidant drug and can be exploited as a source of controlled green-heavy metal cleaner. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acacia%20decurrens" title="Acacia decurrens">Acacia decurrens</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=DPPH" title=" DPPH"> DPPH</a>, <a href="https://publications.waset.org/abstracts/search?q=ABTS" title=" ABTS"> ABTS</a>, <a href="https://publications.waset.org/abstracts/search?q=hyperaccumulation" title=" hyperaccumulation"> hyperaccumulation</a>, <a href="https://publications.waset.org/abstracts/search?q=Menstruum" title=" Menstruum"> Menstruum</a>, <a href="https://publications.waset.org/abstracts/search?q=ICP-OES" title=" ICP-OES"> ICP-OES</a>, <a href="https://publications.waset.org/abstracts/search?q=GC-MS" title=" GC-MS"> GC-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=UV%2Fvisible" title=" UV/visible"> UV/visible</a> </p> <a href="https://publications.waset.org/abstracts/69369/profiling-antibacterial-and-antioxidant-activity-of-acacia-decurrens-willd-an-invasive-south-africa-tree" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/69369.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">325</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2689</span> Investigation on Porcine Follicular Fluid Protein Pattern of Medium and Large Follicles </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hatairuk%20Tungkasen">Hatairuk Tungkasen</a>, <a href="https://publications.waset.org/abstracts/search?q=Somrudee%20Phetchrid"> Somrudee Phetchrid</a>, <a href="https://publications.waset.org/abstracts/search?q=Suwapat%20Jaidee"> Suwapat Jaidee</a>, <a href="https://publications.waset.org/abstracts/search?q=Supinya%20Yoomak"> Supinya Yoomak</a>, <a href="https://publications.waset.org/abstracts/search?q=Chantana%20Kankamol"> Chantana Kankamol</a>, <a href="https://publications.waset.org/abstracts/search?q=Mayuree%20Pumipaiboon"> Mayuree Pumipaiboon</a>, <a href="https://publications.waset.org/abstracts/search?q=Mayuva%20Areekijseree"> Mayuva Areekijseree </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ovaries of reproductive female pigs were obtained from local slaughterhouses in Nakorn Pathom Province, Thailand. Follicular fluid of medium follicle (5-6 diameters) and large follicles (7-8 mm and 10 mm in diameter) were aspirated and collected by sterile technique and analyzed protein pattern. The follicular fluid protein bands were found by SDS-PAGE which has no protein band in difference compared to standard protein band. So we chose protein band molecular weight 50, 62-65, 75-80, 90, 120-160, and >220 kDa were analyzed by LC/MS/MS. The result was found immunoglobulin gamma chain, keratin, transferrin, heat shock protein, and plasminogen precursor, ceruloplasmin, and hemopexin, and protease, respectively. All proteins play important roles in promotion and regulation on growth and development of reproductive cells. The result of this study found many proteins which were useful and important for in vitro oocyte maturation and embryonic development of cell technology in animals. The further study will be use porcine follicular fluid protein of medium and large follicles as feeder cells in in vitro condition to promote oocyte and embryo maturation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=follicular%20fluid%20protein" title="follicular fluid protein">follicular fluid protein</a>, <a href="https://publications.waset.org/abstracts/search?q=LC%2FMS%2FMS" title=" LC/MS/MS"> LC/MS/MS</a>, <a href="https://publications.waset.org/abstracts/search?q=porcine%20oocyte" title=" porcine oocyte"> porcine oocyte</a>, <a href="https://publications.waset.org/abstracts/search?q=SDS-PAGE" title=" SDS-PAGE"> SDS-PAGE</a> </p> <a href="https://publications.waset.org/abstracts/35366/investigation-on-porcine-follicular-fluid-protein-pattern-of-medium-and-large-follicles" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35366.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">585</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2688</span> Thrombophilic Risk Factors and Pregnancy Complications</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hanan%20Azzam1">Hanan Azzam1</a>, <a href="https://publications.waset.org/abstracts/search?q=Nashwa%20Abousamra1"> Nashwa Abousamra1</a>, <a href="https://publications.waset.org/abstracts/search?q=Amany%20Mansour1"> Amany Mansour1</a>, <a href="https://publications.waset.org/abstracts/search?q=Yaser%20Abd%20El-dayem2"> Yaser Abd El-dayem2</a>, <a href="https://publications.waset.org/abstracts/search?q="></a>, <a href="https://publications.waset.org/abstracts/search?q=Solafa%20Elsharawy1">Solafa Elsharawy1</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Inherited thrombophilias are a heterogenous group of conditions which have been implicated in a variety of pregnancy complications. More recently, deficiency of protein Z (PZ) has been liked to pregnancy complications, including preterm delivery. Aim: We designed this study to evaluate the association of inherited thrombophilias including [Protein C (PC), Protein S (PS), Anti thrombin III (ATIII) deficiency and activated protein C (APC) resistance] and protein Z deficiency with a variety of pregnancy complications. Patients and Methods: 60 women with different pregnancy complications, including 20 patients with preeclampsia, 20 patients with intrauterine growth resistance (IUGR), and 20 patients with intrauterine fetal death (IUFD), in addition to 30 healthy pregnant women were recruited for the present study. PC and free PS antigen, ATIII activity, modified functional APC-resistance, and PZ levels were determined. Results: There was no significant association between inherited thrombophilias and complicated pregnancies as regards PC deficiency (p=1.0), AT III and PS deficiency (p=0.312), and APC-resistance (P=0.083). PZ was significantly associated with complicated pregnancies (p=0.012). Patients with protein Z levels below 1.5 µg/ml were considered deficient. Accordingly, we demonstrated protein Z deficiency in 30% of complicated pregnancies (RR 6.0, 95% CI 1.29-27.90;p=0.022), 20% of preeclampsia (RR 3.5, 95% CI 0.57 – 21.28; P = 0.174), 40% of IUGR (RR 9.3 95% CI 1.72-50.61; P = 0.010) and 30% of IUFD (RR 6, 95% CI 1.07 – 33.64; P = 0.042). Conclusions: These findings indicate the absence of association of inherited thrombophilias, including PC, PS, AT III deficiency, and APC resistance with pregnancy complications. However, PZ deficiency is associated with increased risk of pregnancy complications, especially intrauterine growth restriction and intrauterine fetal death. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=protein%20C" title="protein C">protein C</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20S" title=" protein S"> protein S</a>, <a href="https://publications.waset.org/abstracts/search?q=thrombophelia" title=" thrombophelia"> thrombophelia</a>, <a href="https://publications.waset.org/abstracts/search?q=pregnancy" title=" pregnancy"> pregnancy</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20Z" title=" protein Z"> protein Z</a> </p> <a href="https://publications.waset.org/abstracts/144318/thrombophilic-risk-factors-and-pregnancy-complications" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144318.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">234</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2687</span> Characterization of High Carbon Ash from Pulp and Paper mill for Potential Utilization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ruma%20Rano">Ruma Rano</a>, <a href="https://publications.waset.org/abstracts/search?q=Firoza%20Sultana"> Firoza Sultana</a>, <a href="https://publications.waset.org/abstracts/search?q=Bishal%20Bhuyan"> Bishal Bhuyan</a>, <a href="https://publications.waset.org/abstracts/search?q=Nurul%20Alam%20Mazumder"> Nurul Alam Mazumder</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fly ash collected from Cachar Paper Mill, Assam, India has been thoroughly characterized in respect of its physico-chemical, morphological and mineralogical features were concerned by using density, LOI, FTIR, XRD, SEM-EDS etc. The results reveal that there is a striking difference in the features and properties of the coarser and finer fractions .The high carbon ash consists of large unburnt carbon (chars), irregular carbonaceous particles in the coarser fraction, which appear to be porous and may be used as domestic fuel. The percentage of char albeit the carbon content decreases with decrease in size of particles. The various fractions essentially contain quartz and mullite as the main mineral phases. For suggesting the potential utilization channels, number of experiments were performed correlating the total characteristic features. Water holding capacities of different size classified fractions were determined, the coarser fractions have unexpectedly higher water holding capacities than the finer ones. An attempt has been made to correlate the results obtained with potential use in agriculture. Another potential application of coarser particles is used as adsorbent for effluents containing waste organic materials. Thus thorough characterization leads to not only a definite direction about the uses of the value added components but also gives useful information regarding the prevailing combustion process. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chars" title="chars">chars</a>, <a href="https://publications.waset.org/abstracts/search?q=porous" title=" porous"> porous</a>, <a href="https://publications.waset.org/abstracts/search?q=water%20holding%20capacity" title=" water holding capacity"> water holding capacity</a>, <a href="https://publications.waset.org/abstracts/search?q=combustion%20process" title=" combustion process"> combustion process</a> </p> <a href="https://publications.waset.org/abstracts/16433/characterization-of-high-carbon-ash-from-pulp-and-paper-mill-for-potential-utilization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16433.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">363</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2686</span> Transfer Learning for Protein Structure Classification at Low Resolution</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alexander%20Hudson">Alexander Hudson</a>, <a href="https://publications.waset.org/abstracts/search?q=Shaogang%20Gong"> Shaogang Gong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Structure determination is key to understanding protein function at a molecular level. Whilst significant advances have been made in predicting structure and function from amino acid sequence, researchers must still rely on expensive, time-consuming analytical methods to visualise detailed protein conformation. In this study, we demonstrate that it is possible to make accurate (≥80%) predictions of protein class and architecture from structures determined at low (>3A) resolution, using a deep convolutional neural network trained on high-resolution (≤3A) structures represented as 2D matrices. Thus, we provide proof of concept for high-speed, low-cost protein structure classification at low resolution, and a basis for extension to prediction of function. We investigate the impact of the input representation on classification performance, showing that side-chain information may not be necessary for fine-grained structure predictions. Finally, we confirm that high resolution, low-resolution and NMR-determined structures inhabit a common feature space, and thus provide a theoretical foundation for boosting with single-image super-resolution. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=transfer%20learning" title="transfer learning">transfer learning</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20distance%20maps" title=" protein distance maps"> protein distance maps</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20structure%20classification" title=" protein structure classification"> protein structure classification</a>, <a href="https://publications.waset.org/abstracts/search?q=neural%20networks" title=" neural networks"> neural networks</a> </p> <a href="https://publications.waset.org/abstracts/129704/transfer-learning-for-protein-structure-classification-at-low-resolution" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/129704.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">136</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2685</span> Potential Use of Cnidoscolus Chayamansa Leaf from Mexico as High-Quality Protein Source</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Diana%20Karina%20Baigts%20Allende">Diana Karina Baigts Allende</a>, <a href="https://publications.waset.org/abstracts/search?q=Mariana%20%20Gonzalez%20Diaz"> Mariana Gonzalez Diaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Luis%20Antonio%20Chel%20Guerrero"> Luis Antonio Chel Guerrero</a>, <a href="https://publications.waset.org/abstracts/search?q=Mukthar%20Sandoval%20Peraza"> Mukthar Sandoval Peraza</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Poverty and food insecurity are still incident problems in the developing countries, where population´s diet is based on cereals which are lack in protein content. Nevertheless, during last years the use of native plants has been studied as an alternative source of protein in order to improve the nutritional intake. Chaya crop also called Spinach tree, is a prehispanic plant native from Central America and South of Mexico (Mayan culture), which has been especially valued due to its high nutritional content particularly protein and some medicinal properties. The aim of this work was to study the effect of protein isolation processing from Chaya leaf harvest in Yucatan, Mexico on its structure quality in order: i) to valorize the Chaya crop and ii) to produce low-cost and high-quality protein. Chaya leaf was extruded, clarified and recovered using: a) acid precipitation by decreasing the pH value until reach the isoelectric point (3.5) and b) thermal coagulation, by heating the protein solution at 80 °C during 30 min. Solubilized protein was re-dissolved in water and spray dried. The presence of Fraction I protein, known as RuBisCO (Rubilose-1,5-biphosfate carboxylase/oxygenase) was confirmed by gel electrophoresis (SDS-PAGE) where molecular weight bands of 55 KDa and 12 KDa were observed. The infrared spectrum showed changes in protein structure due to the isolation method. The use of high temperatures (thermal coagulation) highly decreased protein solubility in comparison to isoelectric precipitated protein, the nutritional properties according to amino acid profile was also disturbed, showing minor amounts of overall essential amino acids from 435.9 to 367.8 mg/g. Chaya protein isolate obtained by acid precipitation showed higher protein quality according to essential amino acid score compared to FAO recommendations, which could represent an important sustainable source of protein for human consumption. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chaya%20leaf" title="chaya leaf">chaya leaf</a>, <a href="https://publications.waset.org/abstracts/search?q=nutritional%20properties" title=" nutritional properties"> nutritional properties</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20isolate" title=" protein isolate"> protein isolate</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20structure" title=" protein structure"> protein structure</a> </p> <a href="https://publications.waset.org/abstracts/56439/potential-use-of-cnidoscolus-chayamansa-leaf-from-mexico-as-high-quality-protein-source" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56439.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">341</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2684</span> Protein Tertiary Structure Prediction by a Multiobjective Optimization and Neural Network Approach</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alexandre%20Barbosa%20de%20Almeida">Alexandre Barbosa de Almeida</a>, <a href="https://publications.waset.org/abstracts/search?q=Telma%20Woerle%20de%20Lima%20Soares"> Telma Woerle de Lima Soares</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Protein structure prediction is a challenging task in the bioinformatics field. The biological function of all proteins majorly relies on the shape of their three-dimensional conformational structure, but less than 1% of all known proteins in the world have their structure solved. This work proposes a deep learning model to address this problem, attempting to predict some aspects of the protein conformations. Throughout a process of multiobjective dominance, a recurrent neural network was trained to abstract the particular bias of each individual multiobjective algorithm, generating a heuristic that could be useful to predict some of the relevant aspects of the three-dimensional conformation process formation, known as protein folding. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ab%20initio%20heuristic%20modeling" title="Ab initio heuristic modeling">Ab initio heuristic modeling</a>, <a href="https://publications.waset.org/abstracts/search?q=multiobjective%20optimization" title=" multiobjective optimization"> multiobjective optimization</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20structure%20prediction" title=" protein structure prediction"> protein structure prediction</a>, <a href="https://publications.waset.org/abstracts/search?q=recurrent%20neural%20network" title=" recurrent neural network"> recurrent neural network</a> </p> <a href="https://publications.waset.org/abstracts/141565/protein-tertiary-structure-prediction-by-a-multiobjective-optimization-and-neural-network-approach" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/141565.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">205</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2683</span> Effect of 17α-Methyltestosterone Hormone on Haematological Profiles of the Sex Reversed, Sarotherodon Melanotheron</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ayoola">Ayoola</a>, <a href="https://publications.waset.org/abstracts/search?q=Simeon%20Oluwatoyin"> Simeon Oluwatoyin</a>, <a href="https://publications.waset.org/abstracts/search?q=Omogoriola%20Hannah%20Omoloye"> Omogoriola Hannah Omoloye </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The effects of 17α-Methyltestosterone Hormone on blood composition of the Sex Reversed Sarotherodon melanotheron were investigated. S. melanotheron fry were reared in six (6) plastic tanks for three (3) months, of which three (3) tanks served as treatment tanks while the other three (3) served as the control. The fry were fed with 17α-methyl testosterone enzyme, which functions as a sex reversal hormone. The fry were administered this hormone for 30 days, to ensure complete sex reversal. All the S. melanotheron fry were reared to table size for duration of three (3) months, after which, blood samples were taken from both the control and treatment fishes. The blood parameters showed no significant differences with the same values of White Blood Cell count (WBC) and Total plasma protein for the control and experimental fishes. A total protein value for sex reversed specimens was 3.99g/dL, while urea and creatinine values were 0.2g/dL. Alkaline Phosphatase, Aspartate transaminase and Alanine transaminase for the treatment specimen were 183nm/mg protein/min, 98nm/mg protein/min and 105nm/mg protein/min respectively. A total protein value for control specimens was 2.81g/dL, while urea and creatinine values were 0.2g/dL. Alkaline Phosphatase, Aspartate transaminase and Alanine transaminase for the control species were 174nm/mg protein/min, 93nm/mg protein/min and 106nm/mg protein/min respectively. The safety of MT on S. melanotheron is therefore proved since there is no adverse effect on the fish. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=17%CE%B1-Methyltestosterone" title="17α-Methyltestosterone">17α-Methyltestosterone</a>, <a href="https://publications.waset.org/abstracts/search?q=haematology" title=" haematology"> haematology</a>, <a href="https://publications.waset.org/abstracts/search?q=sex%20reversal" title=" sex reversal"> sex reversal</a>, <a href="https://publications.waset.org/abstracts/search?q=sarotherodon%20melanotheron" title=" sarotherodon melanotheron "> sarotherodon melanotheron </a> </p> <a href="https://publications.waset.org/abstracts/29481/effect-of-17a-methyltestosterone-hormone-on-haematological-profiles-of-the-sex-reversed-sarotherodon-melanotheron" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29481.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">492</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2682</span> The Impact of Different Rhizobium leguminosarum Strains on the Protein Content of Peas and Broad Beans</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alise%20Senberga">Alise Senberga</a>, <a href="https://publications.waset.org/abstracts/search?q=Laila%20Dubova"> Laila Dubova</a>, <a href="https://publications.waset.org/abstracts/search?q=Liene%20Strauta"> Liene Strauta</a>, <a href="https://publications.waset.org/abstracts/search?q=Ina%20Alsina"> Ina Alsina</a>, <a href="https://publications.waset.org/abstracts/search?q=Ieva%20Erdberga"> Ieva Erdberga</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Legume symbiotic relationship with nitrogen fixating bacteria Rhizobim leguminosarum is an important factor used to improve the productivity of legumes, due to the fact that rhizobia can supply plant with the necessary amount of nitrogen. R. leguminosarum strains have shown different activity in fixing nitrogen. Depending on the chosen R. leguminosarum strain, host plant biochemical content can be altered. In this study we focused particularly on the changes in protein content in beans (using two different varieties) and peas (five different varieties) due to the use of several different R. leguminosarum strains (four strains for both beans and peas). Overall, the protein content increase was observed after seed inoculation with R. leguminosarum. Strain and plant cultivar interaction specification was observed. The effect of R. leguminosarum inoculation on the content of protein was dependent on the R. leguminosarum strain used. Plant cultivar also appeared to have a decisive role in protein content formation with the help of R. leguminosaru. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=legumes" title="legumes">legumes</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20content" title=" protein content"> protein content</a>, <a href="https://publications.waset.org/abstracts/search?q=rhizobia%20strains" title=" rhizobia strains"> rhizobia strains</a>, <a href="https://publications.waset.org/abstracts/search?q=soil" title=" soil"> soil</a> </p> <a href="https://publications.waset.org/abstracts/27686/the-impact-of-different-rhizobium-leguminosarum-strains-on-the-protein-content-of-peas-and-broad-beans" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27686.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">522</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2681</span> Text Mining Techniques for Prioritizing Pathogenic Mutations in Protein Families Known to Misfold or Aggregate</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Khaleel%20Saleh%20Al-Rababah">Khaleel Saleh Al-Rababah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Amyloid fibril forming regions, which are known as protein aggregates, in sequences of some protein families are associated with a number of diseases known as amyloidosis. Mutations play a role in forming fibrils by accelerating the fibril formation process. In this paper we want to extract diseases that caused by those mutations as a result of the impact of the mutations on structural and functional properties of the aggregated protein. We propose a text mining system, to automatically extract mutations, diseases and relations between mutations and diseases. We presented an algorithm based on finite state to cluster mutations found in the same sentence as a sentence could contain different mutation cause different diseases. Also, we presented a co reference algorithm that enables cross-link sentences. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=amyloid" title="amyloid">amyloid</a>, <a href="https://publications.waset.org/abstracts/search?q=amyloidosis" title=" amyloidosis"> amyloidosis</a>, <a href="https://publications.waset.org/abstracts/search?q=co%20reference" title=" co reference"> co reference</a>, <a href="https://publications.waset.org/abstracts/search?q=protein" title=" protein"> protein</a>, <a href="https://publications.waset.org/abstracts/search?q=text%20mining" title=" text mining"> text mining</a> </p> <a href="https://publications.waset.org/abstracts/24232/text-mining-techniques-for-prioritizing-pathogenic-mutations-in-protein-families-known-to-misfold-or-aggregate" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24232.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">526</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2680</span> Optimising Light Conditions for Recombinant Protein Production in the Microalgal Chlamydomonas reinhardtii Chloroplast</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saskya%20E.%20Carrera%20P.">Saskya E. Carrera P.</a>, <a href="https://publications.waset.org/abstracts/search?q=Ben%20Hankamer"> Ben Hankamer</a>, <a href="https://publications.waset.org/abstracts/search?q=Melanie%20Oey"> Melanie Oey</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The green alga C. reinhardtii provides a platform for the cheap, scalable, and safe production of complex proteins. Despite gene expression in photosynthetic organisms being tightly regulated by light, most expression studies have analysed chloroplast recombinant protein production under constant light. Here the influence of illumination time and intensity on GFP and a GFP-PlyGBS (bacterial-lysin) fusion protein expression was investigated. The expression of both proteins was strongly influenced by the light regime (6-24 hr illumination per day), the light intensity (0-450 E m⁻²s⁻¹) and growth condition (photoautotrophic, mixotrophic and heterotrophic). Heterotrophic conditions resulted in relatively low recombinant protein yields per unit volume, despite high protein yields per cell, due to low growth rates. Mixotrophic conditions exhibited the highest yields at 6 hrs illumination at 200µE m⁻²s⁻¹ and under continuous low light illumination (13-16 mg L⁻¹ GFP and 1.2-1.6 mg L⁻¹ GFP-PlyGBS), as these conditions supported good cell growth and cellular protein yields. A ~23-fold increase in protein accumulation per cell and ~9-fold increase L⁻¹ culture was observed compared to standard constant 24 hr illumination for GFP-PlyGBS. The highest yields under photoautotrophic conditions were obtained under 9 hrs illumination (6 mg L⁻¹ GFP and 2.1 mg L⁻¹ GFP-PlyGBS). This represents a ~4-fold increase in cellular protein accumulation for GFP-PlyGBS. On a volumetric basis the highest yield was at 15 hrs illumination (~2-fold increase L⁻¹ over the constant light for GFP-PlyGBS). Optimising illumination conditions to balance growth and protein expression can thus significantly enhance overall recombinant protein production in C. reinhardtii cultures. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chlamydomonas%20reinhardtii" title="chlamydomonas reinhardtii">chlamydomonas reinhardtii</a>, <a href="https://publications.waset.org/abstracts/search?q=light" title=" light"> light</a>, <a href="https://publications.waset.org/abstracts/search?q=mixotrophic" title=" mixotrophic"> mixotrophic</a>, <a href="https://publications.waset.org/abstracts/search?q=recombinant%20protein" title=" recombinant protein"> recombinant protein</a> </p> <a href="https://publications.waset.org/abstracts/84908/optimising-light-conditions-for-recombinant-protein-production-in-the-microalgal-chlamydomonas-reinhardtii-chloroplast" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/84908.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">255</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2679</span> Effect of Crude oil Contamination on the Morphological Traits and Protein Content of Avicennia Marina</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Babak%20Moradi">Babak Moradi</a>, <a href="https://publications.waset.org/abstracts/search?q=Hassan%20Zare-Maivan"> Hassan Zare-Maivan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A greenhouse investigation has been conducted to study the effect of crude oil on morphology and protein content of Avicennia marina plant. Avicennia marina seeds were sown in different concentrations of the crude oil mixed soil (i.e., 2.5, 5, 7.5, and 10 w/w). Controls and replicates were also set up. Morphological traits were recorded 4 months after plantation. Avicennia marina seedlings could tolerate up to 10% (w/w). Results demonstrated that there was a reduction in plant shoot and root biomass with the increase of crude oil concentration. Plant height, total leaf number and length reduced significantly with increase of crude oil contamination. Investigation revealed that there is a great impact of crude oil contamination on protein content of the roots of the experimental plant. Protein content of roots grown in different concentrations of crude oil were more than those of the control plant. Further, results also showed that protein content was increased with increased concentration of crude oil. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Avicennia%20marina" title="Avicennia marina">Avicennia marina</a>, <a href="https://publications.waset.org/abstracts/search?q=morphology" title=" morphology"> morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=oil%20contamination" title=" oil contamination"> oil contamination</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20content" title=" protein content"> protein content</a> </p> <a href="https://publications.waset.org/abstracts/23576/effect-of-crude-oil-contamination-on-the-morphological-traits-and-protein-content-of-avicennia-marina" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23576.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">376</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2678</span> Protein Feeding Pattern, Casein Feeding, or Milk-Soluble Protein Feeding did not Change the Evolution of Body Composition during a Short-Term Weight Loss Program</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Solange%20Adechian">Solange Adechian</a>, <a href="https://publications.waset.org/abstracts/search?q=Mich%C3%A8le%20Balage"> Michèle Balage</a>, <a href="https://publications.waset.org/abstracts/search?q=Didier%20Remond"> Didier Remond</a>, <a href="https://publications.waset.org/abstracts/search?q=Carole%20Mign%C3%A9"> Carole Migné</a>, <a href="https://publications.waset.org/abstracts/search?q=Annie%20Quignard-Boulang%C3%A9"> Annie Quignard-Boulangé</a>, <a href="https://publications.waset.org/abstracts/search?q=Agn%C3%A8s%20Marset-Baglieri"> Agnès Marset-Baglieri</a>, <a href="https://publications.waset.org/abstracts/search?q=Sylvie%20Rousset"> Sylvie Rousset</a>, <a href="https://publications.waset.org/abstracts/search?q=Yves%20Boirie"> Yves Boirie</a>, <a href="https://publications.waset.org/abstracts/search?q=Claire%20Gaudichon"> Claire Gaudichon</a>, <a href="https://publications.waset.org/abstracts/search?q=Dominique%20Dardevet"> Dominique Dardevet</a>, <a href="https://publications.waset.org/abstracts/search?q=Laurent%20Mosoni"> Laurent Mosoni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Studies have shown that timing of protein intake, leucine content, and speed of digestion significantly affect postprandial protein utilization. Our aim was to determine if one can spare lean body mass during energy restriction by varying the quality and the timing of protein intake. Obese volunteers followed a 6-wk restricted energy diet. Four groups were compared: casein pulse, casein spread, milk-soluble protein (MSP, = whey) pulse, and MSP spread (n = 10-11 per group). In casein groups, caseins were the only protein source; it was MSP in MSP groups. Proteins were distributed in four meals per day in the proportion 8:80:4:8% in the pulse groups; it was 25:25:25:25% in the spread groups. We measured weight, body composition, nitrogen balance, 3-methylhistidine excretion, perception of hunger, plasma parameters, adipose tissue metabolism, and whole body protein metabolism. Volunteers lost 7.5 ± 0.4 kg of weight, 5.1 ± 0.2 kg of fat, and 2.2 ± 0.2 kg of lean mass, with no difference between groups. In adipose tissue, cell size and mRNA expression of various genes were reduced with no difference between groups. Hunger perception was also never different between groups. In the last week, due to a higher inhibition of protein degradation and despite a lower stimulation of protein synthesis, postprandial balance between whole body protein synthesis and degradation was better with caseins than with MSP. It seems likely that the positive effect of caseins on protein balance occurred only at the end of the experiment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lean%20body%20mass" title="lean body mass">lean body mass</a>, <a href="https://publications.waset.org/abstracts/search?q=fat%20mass" title=" fat mass"> fat mass</a>, <a href="https://publications.waset.org/abstracts/search?q=casein" title=" casein"> casein</a>, <a href="https://publications.waset.org/abstracts/search?q=whey" title=" whey"> whey</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20metabolism" title=" protein metabolism"> protein metabolism</a> </p> <a href="https://publications.waset.org/abstracts/175765/protein-feeding-pattern-casein-feeding-or-milk-soluble-protein-feeding-did-not-change-the-evolution-of-body-composition-during-a-short-term-weight-loss-program" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/175765.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">72</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2677</span> The Potential of Acanthaster Plancii Fractions as Anti-Atherosclerotic Agent by Inhibiting the Expression of Proprotein Convertase Subtilisin-Kexin Type 9</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nurjannatul%20Naim%20Kamaruddin">Nurjannatul Naim Kamaruddin</a>, <a href="https://publications.waset.org/abstracts/search?q=Tengku%20Sifziuzl%20Tengku%20Muhammad"> Tengku Sifziuzl Tengku Muhammad</a>, <a href="https://publications.waset.org/abstracts/search?q=Aina%20Farahiyah%20Abdul%20Manan"> Aina Farahiyah Abdul Manan</a>, <a href="https://publications.waset.org/abstracts/search?q=Habsah%20Mohamad"> Habsah Mohamad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Atherosclerosis which leads to cardiovascular diseases such as myocardial infarction, unstable angina (ischemic heart pain), sudden cardiac death and stroke is the principal cause of death worldwide. It has been a very critical issue as current common drug treatment, statin therapy has left bad side effects like rhabdomyolysis, atrial fibrillation, liver disease, abdominal and chest pain. Interestingly, the discoveries of proprotein convertase subtilisin-kexin type 9 have paved a new way in the treatment of atherosclerosis. This serine protease is believed to involve in the regulation of LDL- uptake by LDL-receptor. Therefore, this study was conducted to evaluate the potential of Acanthaster plancii fractions to reduce the transcriptional activity of the PCSK9 promoter. In this study, the marine organism which is Acanthaster plancii has been used as the source for marine compounds in inhibiting PCSK9. The cytotoxicity activity of ten fractions from the methanol extracts of Acanthaster plancii was investigated on HepG2 cell lines using MTS assay and dual glo luciferase assay was carried out later to analyses the effects of the samples in reducing the transcriptional activity of the PCSK9 promoter. Both assays used fractions with five different concentrations, 3.13µg/mL, 6.25µg/mL, 12.5µg/mL, 25µg/mL, and 50µg/mL. MTS assay indicated that the fractions are non-cytotoxic towards HepG2 cell lines as their IC50 value is greater than 30µg/mL. Whilst, for the dual glo luciferase assay, among all the fractions, Enhance Fraction 2 (EF2) showed the best potential in reducing the transcriptional activity of the PCSK9 promoter. The results indicated that this EF2 gave the lowest PCSK9 promoter expression at low concentration which is 0.2 fold change at 6.25µg/mL. This finding suggested that further analysis should be done to validate the potential of Acanthaster plancii as the source of anti-atherosclerotic agent. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acanthaster%20plancii" title="Acanthaster plancii">Acanthaster plancii</a>, <a href="https://publications.waset.org/abstracts/search?q=atherosclerosis" title=" atherosclerosis"> atherosclerosis</a>, <a href="https://publications.waset.org/abstracts/search?q=luciferase%20assay" title=" luciferase assay"> luciferase assay</a>, <a href="https://publications.waset.org/abstracts/search?q=PCSK9" title=" PCSK9"> PCSK9</a> </p> <a href="https://publications.waset.org/abstracts/96387/the-potential-of-acanthaster-plancii-fractions-as-anti-atherosclerotic-agent-by-inhibiting-the-expression-of-proprotein-convertase-subtilisin-kexin-type-9" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96387.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">147</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2676</span> Functionality and Application of Rice Bran Protein Hydrolysates in Oil in Water Emulsions: Their Stabilities to Environmental Stresses</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=R.%20Charoen">R. Charoen</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Tipkanon"> S. Tipkanon</a>, <a href="https://publications.waset.org/abstracts/search?q=W.%20Savedboworn"> W. Savedboworn</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Phonsatta"> N. Phonsatta</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Panya"> A. Panya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rice bran protein hydrolysates (RBPH) were prepared from defatted rice bran of two different Thai rice cultivars (Plai-Ngahm-Prachinburi; PNP and Khao Dok Mali 105; KDM105) using an enzymatic method. This research aimed to optimize enzyme-assisted protein extraction. In addition, the functional properties of RBPH and their stabilities to environmental stresses including pH (3 to 8), ionic strength (0 mM to 500 mM) and the thermal treatment (30 °C to 90 °C) were investigated. Results showed that enzymatic process for protein extraction of defatted rice bran was as follows: enzyme concentration 0.075 g/ 5 g of protein, extraction temperature 50 °C and extraction time 4 h. The obtained protein hydrolysate powders had a degree of hydrolysis (%) of 21.05% in PNP and 19.92% in KDM105. The solubility of protein hydrolysates at pH 4-6 was ranged from 27.28-38.57% and 27.60-43.00% in PNP and KDM105, respectively. In general, antioxidant activities indicated by total phenolic content, FRAP, ferrous ion-chelating (FIC), and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) of KDM105 had higher than PNP. In terms of functional properties, the emulsifying activity index (EAI) was was 8.78 m²/g protein in KDM105, whereas PNP was 5.05 m²/g protein. The foaming capacity at 5 minutes (%) was 47.33 and 52.98 in PNP and KDM105, respectively. Glutamine, Alanine, Valine, and Leucine are the major amino acid in protein hydrolysates where the total amino acid of KDM105 gave higher than PNP. Furthermore, we investigated environmental stresses on the stability of 5% oil in water emulsion (5% oil, 10 mM citrate buffer) stabilized by RBPH (3.5%). The droplet diameter of emulsion stabilized by KDM105 was smaller (d < 250 nm) than produced by PNP. For environmental stresses, RBPH stabilized emulsions were stable at pH around 3 and 5-6, at high salt (< 400 mM, pH 7) and at temperatures range between 30-50°C. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=functional%20properties" title="functional properties">functional properties</a>, <a href="https://publications.waset.org/abstracts/search?q=oil%20in%20water%20emulsion" title=" oil in water emulsion"> oil in water emulsion</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20hydrolysates" title=" protein hydrolysates"> protein hydrolysates</a>, <a href="https://publications.waset.org/abstracts/search?q=rice%20bran%20protein" title=" rice bran protein"> rice bran protein</a> </p> <a href="https://publications.waset.org/abstracts/82295/functionality-and-application-of-rice-bran-protein-hydrolysates-in-oil-in-water-emulsions-their-stabilities-to-environmental-stresses" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/82295.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">218</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2675</span> Physicochemical and Functional Characteristics of Hemp Protein Isolate</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=El-Sohaimy%20Sobhy%20A.">El-Sohaimy Sobhy A.</a>, <a href="https://publications.waset.org/abstracts/search?q=Androsova%20Natalia"> Androsova Natalia</a>, <a href="https://publications.waset.org/abstracts/search?q=Toshev%20Abuvali%20Djabarovec"> Toshev Abuvali Djabarovec</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The conditions of the isolation of proteins from the hemp seeds were optimized in the current work. Moreover, the physicochemical and functional properties of hemp protein isolate were evaluated for its potential application in food manufacturing. The elastin protein is the most predominant protein in the protein profile with a molecular weight of 58.1 KDa, besides albumin, with a molecular weight of 31.5 KDa. The FTIR spectrum detected the absorption peaks of the amide I in 1750 and 1600 cm⁻¹, which pointed to C=O stretching while N-H was stretching at 1650-1580 cm⁻¹. The peak at 3250 was related to N-H stretching of primary aliphatic amine (3400-3300 cm⁻¹), and the N-H stretching for secondary (II) amine appeared at 3350-3310 cm⁻¹. Hemp protein isolate (HPI) was showed high content of arginine (15.52 g/100 g), phenylalanine+tyrosine (9.63 g/100 g), methionine + cysteine (5.49 g/100 g), leucine + isoleucine (5.21 g/100 g) and valine (4.53 g/100 g). It contains a moderate level of threonine (3.29 g/100 g) and lysine (2.50 g/100 g), with the limiting amino acid being a tryptophan (0.22 g/100 g HPI). HPI showed high water-holding capacity (4.5 ± 2.95 ml/g protein) and oil holding capacity (2.33 ± 1.88 ml/g) values. The foaming capacity of HPI was increased with increasing the pH values to reach the maximum value at pH 11 (67.23±3.20 %). The highest emulsion ability index of HPI was noted at pH 9 (91.3±2.57 m2/g) with low stability (19.15±2.03). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cannabis%20sativa%20ssp." title="Cannabis sativa ssp.">Cannabis sativa ssp.</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20isolate" title=" protein isolate"> protein isolate</a>, <a href="https://publications.waset.org/abstracts/search?q=isolation%20conditions" title=" isolation conditions"> isolation conditions</a>, <a href="https://publications.waset.org/abstracts/search?q=amino%20acid%20composition" title=" amino acid composition"> amino acid composition</a>, <a href="https://publications.waset.org/abstracts/search?q=chemical%20properties" title=" chemical properties"> chemical properties</a>, <a href="https://publications.waset.org/abstracts/search?q=functional%20properties" title=" functional properties"> functional properties</a> </p> <a href="https://publications.waset.org/abstracts/150400/physicochemical-and-functional-characteristics-of-hemp-protein-isolate" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/150400.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">180</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2674</span> Physicochemical and Antioxidative Characteristics of Black Bean Protein Hydrolysates Obtained from Different Enzymes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zhaojun%20Zheng">Zhaojun Zheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuanfa%20Liu"> Yuanfa Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Jiaxin%20Li"> Jiaxin Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Jinwei%20Li"> Jinwei Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Yong-jiang%20Xu"> Yong-jiang Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Chen%20Cao"> Chen Cao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Black bean is an excellent protein source for preparing hydrolysates, which attract much attention due to their biological activity. The objective of this study was to characterize the physicochemical and antioxidant properties of black bean protein, hydrolyzed by ficin, bromelain or alcalase until 300 min of hydrolysis. Results showed that bromelain and alcalase hydrolysates possessed a higher degree of hydrolysis (DH) than that of ficin, thereby presenting different ultraviolet absorption, fluorescence intensity, and circular dichroism. Moreover, all hydrolysates possessed the capacity to scavenge DPPH radical with the lowest IC₅₀ of 21.11 µg/mL, as well as to chelate ferrous ion (Fe²⁺) with the IC₅₀ values ranging from 6.82 to 30.68 µg/mL. Intriguingly, the oxidation of linoleic acid, sunflower oil, and sunflower oil-in-water emulsion was remarkedly retarded by the three selected protein hydrolysates, especially by bromelain-treated protein hydrolysate, which might attribute to their high hydrophobicity and emulsifying properties. These findings can provide strong support for black bean protein hydrolysates to be employed in food products acting as natural antioxidant alternatives. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title="antioxidant activity">antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=black%20bean%20protein%20hydrolysate" title=" black bean protein hydrolysate"> black bean protein hydrolysate</a>, <a href="https://publications.waset.org/abstracts/search?q=emulsion%20physicochemical%20properties" title=" emulsion physicochemical properties"> emulsion physicochemical properties</a>, <a href="https://publications.waset.org/abstracts/search?q=sunflower%20oil" title=" sunflower oil"> sunflower oil</a> </p> <a href="https://publications.waset.org/abstracts/105885/physicochemical-and-antioxidative-characteristics-of-black-bean-protein-hydrolysates-obtained-from-different-enzymes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/105885.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">137</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2673</span> Production and Purification of Salmonella Typhimurium MisL Autotransporter Protein in Escherichia coli</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Neslihan%20Taskale%20Karatug">Neslihan Taskale Karatug</a>, <a href="https://publications.waset.org/abstracts/search?q=Mustafa%20Akcelik"> Mustafa Akcelik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Some literature data show that misL protein play a role on host immune response formed against Salmonella Typhimurium. The aim of the present study is to learn the role of the protein in S. Typhimurium pathogenicity. To describe certain functions of the protein, primarily recombinant misL protein was produced and purified. PCR was performed using a primer set targeted to passenger domain of the misL gene on S. Typhimurium LT2 genome. Amplicon and pet28a vector were enzymatically cleaved with EcoRI and NheI. The digested DNA materials were purified with High Pure PCR Product Purification Kit. The ligation reaction was achieved with the pure products. After preparation of competent Escherichia coli Dh5α, ligation mix was transformed into the cell by electroporation. To confirm the existence of insert gene, recombinant plasmid DNA of Dh5α was isolated with high pure plasmid DNA kit. Proved the correctness of recombinant plasmid was electroporated to BL21. The cell was induced by IPTG. After induction, the presence of recombinant protein was checked by SDS-PAGE. The recombinant misL protein was purified using HisPur Ni-NTA spin colon. The pure protein was shown by SDS-PAGE and western blot immünoassay. The concentration of the protein was measured BCA Protein Assay kit. In the wake of ligation with digested products (2 kb misL and 5.4 kb pet28a) visualised on gel size of the band was about 7.4 kb and was named as pNT01. The pNT01 recombinant plasmid was transformed into Dh5α and colonies were chosen in selective medium. Plasmid DNA isolation from them was carried out. PCR was achieved on the pNT01 to check misL and 2 kb band was observed on the agarose gel. After electroporation of the plasmid and induction of the cell, 68 kDa misL protein was seen. Subsequent to the purification of the protein, only a band was observed on SDS-PAGE. Association of the pure protein with anti-his antibody was verified by the western blot assay. The concentration of the pure misL protein was determined as 345 μg/mL. Production of polyclonal antibody will be achieved by using the obtained pure recombinant misL protein as next step. The role of the protein will come out on the immune system together some assays. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cloning" title="cloning">cloning</a>, <a href="https://publications.waset.org/abstracts/search?q=Escherichia%20coli" title=" Escherichia coli"> Escherichia coli</a>, <a href="https://publications.waset.org/abstracts/search?q=recombinant%20protein%20purification" title=" recombinant protein purification"> recombinant protein purification</a>, <a href="https://publications.waset.org/abstracts/search?q=Salmonella%20Typhimurium" title=" Salmonella Typhimurium"> Salmonella Typhimurium</a> </p> <a href="https://publications.waset.org/abstracts/22958/production-and-purification-of-salmonella-typhimurium-misl-autotransporter-protein-in-escherichia-coli" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22958.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">391</span> </span> </div> </div> <ul class="pagination"> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=2" rel="prev">&lsaquo;</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=1">1</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=2">2</a></li> <li class="page-item active"><span class="page-link">3</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=6">6</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=7">7</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=8">8</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=9">9</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=10">10</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=92">92</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=93">93</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=protein%20fractions&page=4" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); });*/ jQuery.get({ url: "https://publications.waset.org/xhr/user-menu", cache: false }).then(function(response){ jQuery('#mainNavMenu').append(response); }); }); </script> </body> </html>