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overflow: hidden; text-overflow: ellipsis; -webkit-line-clamp: 3; -webkit-box-orient: vertical; }</style><div class="col-xs-12 clearfix"><div class="u-floatLeft"><h1 class="PageHeader-title u-m0x u-fs30">Microscopy</h1><div class="u-tcGrayDark">23,412 Followers</div><div class="u-tcGrayDark u-mt2x">Recent papers in <b>Microscopy</b></div></div></div></div></div></div><div class="TabbedNavigation"><div class="container"><div class="row"><div class="col-xs-12 clearfix"><ul class="nav u-m0x u-p0x list-inline u-displayFlex"><li class="active"><a href="https://www.academia.edu/Documents/in/Microscopy">Top Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Microscopy/MostCited">Most Cited Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Microscopy/MostDownloaded">Most Downloaded Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Microscopy/MostRecent">Newest Papers</a></li><li><a class="" href="https://www.academia.edu/People/Microscopy">People</a></li></ul></div><style type="text/css">ul.nav{flex-direction:row}@media(max-width: 567px){ul.nav{flex-direction:column}.TabbedNavigation li{max-width:100%}.TabbedNavigation li.active{background-color:var(--background-grey, #dddde2)}.TabbedNavigation li.active:before,.TabbedNavigation li.active:after{display:none}}</style></div></div></div><div class="container"><div class="row"><div class="col-xs-12"><div class="u-displayFlex"><div class="u-flexGrow1"><div class="works"><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_40432986 coauthored" data-work_id="40432986" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/40432986/Phytoliths_parasites_fibers_and_feathers_from_dental_calculus_and_soil_from_Iron_Age_Luistari_cemetery_Finland">Phytoliths, parasites, fibers, and feathers from dental calculus and soil from Iron Age Luistari cemetery, Finland.</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Our understanding of subsistence strategies, resources and lifeways of Finnish Iron Age populations remains incomplete despite archaeological, osteological, macrobotanical, and palynological investigations. This is due in part to poor... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_40432986" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Our understanding of subsistence strategies, resources and lifeways of Finnish Iron Age populations remains incomplete despite archaeological, osteological, macrobotanical, and palynological investigations. This is due in part to poor preservation of organic macroremains in the acidic boreal sediments. To address this problem, here we present the first data from microscopic remains preserved in prehistoric dental calculus from Finland. We extracted and analysed both plant and animal microremains from human calculus and burial site sediment samples, originating from Luistari cemetery in southwestern Finland (samples from c. 600e1200 calAD). We recovered phytoliths, parasites, fibers and feathers. While in Finland few previous archaeological studies have investigated phytoliths, our study confirms the importance of these microremains for interpretating dietary patterns. It is also the first time that intestinal parasites have been reported in Finland.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/40432986" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d4c0266ba8e4e669d63ab44aa3782dcf" rel="nofollow" data-download="{"attachment_id":60771519,"asset_id":40432986,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/60771519/download_file?st=MTc0MDY0ODAyNCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="3309423" href="https://helsinki.academia.edu/TuijaKirkinen">Tuija Kirkinen</a><script data-card-contents-for-user="3309423" type="text/json">{"id":3309423,"first_name":"Tuija","last_name":"Kirkinen","domain_name":"helsinki","page_name":"TuijaKirkinen","display_name":"Tuija Kirkinen","profile_url":"https://helsinki.academia.edu/TuijaKirkinen?f_ri=3091","photo":"https://0.academia-photos.com/3309423/1103248/1375749/s65_tuija.kirkinen.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-40432986">+1</span><div class="hidden js-additional-users-40432986"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/Laakkonen">J. Laakkonen</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-40432986'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-40432986').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_40432986 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="40432986"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 40432986, container: ".js-paper-rank-work_40432986", }); });</script></li><li class="js-percentile-work_40432986 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 40432986; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_40432986"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_40432986 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="40432986"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 40432986; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=40432986]").text(description); $(".js-view-count-work_40432986").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_40432986").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="40432986"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="42197" rel="nofollow" href="https://www.academia.edu/Documents/in/Iron_Age">Iron Age</a>, <script data-card-contents-for-ri="42197" type="text/json">{"id":42197,"name":"Iron Age","url":"https://www.academia.edu/Documents/in/Iron_Age?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="61114" rel="nofollow" href="https://www.academia.edu/Documents/in/Fibers">Fibers</a>, <script data-card-contents-for-ri="61114" type="text/json">{"id":61114,"name":"Fibers","url":"https://www.academia.edu/Documents/in/Fibers?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="63945" rel="nofollow" href="https://www.academia.edu/Documents/in/Microarchaeology">Microarchaeology</a><script data-card-contents-for-ri="63945" type="text/json">{"id":63945,"name":"Microarchaeology","url":"https://www.academia.edu/Documents/in/Microarchaeology?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=40432986]'), work: {"id":40432986,"title":"Phytoliths, parasites, fibers, and feathers from dental calculus and soil from Iron Age Luistari cemetery, Finland.","created_at":"2019-09-24T09:23:55.954-07:00","url":"https://www.academia.edu/40432986/Phytoliths_parasites_fibers_and_feathers_from_dental_calculus_and_soil_from_Iron_Age_Luistari_cemetery_Finland?f_ri=3091","dom_id":"work_40432986","summary":"Our understanding of subsistence strategies, resources and lifeways of Finnish Iron Age populations remains incomplete despite archaeological, osteological, macrobotanical, and palynological investigations. This is due in part to poor preservation of organic macroremains in the acidic boreal sediments. To address this problem, here we present the first data from microscopic remains preserved in prehistoric dental calculus from Finland. We extracted and analysed both plant and animal microremains from human calculus and burial site sediment samples, originating from Luistari cemetery in southwestern Finland (samples from c. 600e1200 calAD). We recovered phytoliths, parasites, fibers and feathers. While in Finland few previous archaeological studies have investigated phytoliths, our study confirms the importance of these microremains for interpretating dietary patterns. It is also the first time that intestinal parasites have been reported in Finland.","downloadable_attachments":[{"id":60771519,"asset_id":40432986,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":3309423,"first_name":"Tuija","last_name":"Kirkinen","domain_name":"helsinki","page_name":"TuijaKirkinen","display_name":"Tuija Kirkinen","profile_url":"https://helsinki.academia.edu/TuijaKirkinen?f_ri=3091","photo":"https://0.academia-photos.com/3309423/1103248/1375749/s65_tuija.kirkinen.jpg"},{"id":63805017,"first_name":"J.","last_name":"Laakkonen","domain_name":"independent","page_name":"Laakkonen","display_name":"J. Laakkonen","profile_url":"https://independent.academia.edu/Laakkonen?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":42197,"name":"Iron Age","url":"https://www.academia.edu/Documents/in/Iron_Age?f_ri=3091","nofollow":true},{"id":61114,"name":"Fibers","url":"https://www.academia.edu/Documents/in/Fibers?f_ri=3091","nofollow":true},{"id":63945,"name":"Microarchaeology","url":"https://www.academia.edu/Documents/in/Microarchaeology?f_ri=3091","nofollow":true},{"id":371994,"name":"Dental Calculus","url":"https://www.academia.edu/Documents/in/Dental_Calculus?f_ri=3091"},{"id":430462,"name":"Feathers","url":"https://www.academia.edu/Documents/in/Feathers?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13039659 coauthored" data-work_id="13039659" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13039659/Age_related_changes_of_dopamine_receptors_in_the_rat_hippocampus_a_light_microscope_autoradiography_study">Age-related changes of dopamine receptors in the rat hippocampus: a light microscope autoradiography study</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Hippocampus is a brain region involved in learning and memory and is particularly sensitive to ageing. It is supplied with a dopaminergic innervation arising from the midbrain, which is part of the mesolimbic dopaminergic pathway.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13039659" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Hippocampus is a brain region involved in learning and memory and is particularly sensitive to ageing. It is supplied with a dopaminergic innervation arising from the midbrain, which is part of the mesolimbic dopaminergic pathway. Dysfunction of the dopaminergic mesolimbic system is probably involved in the pathophysiology of psychosis and behavioural disturbances occurring in the elderly. The present study was designed to assess the density and localisation of dopamine D1-and D2-like receptor subtypes in the hippocampus of male Sprague-Dawley rats aged 3 months (young), 12 months (adult) and 24 months (old). Dopamine D1-like receptors, labelled by [ 3 H]-SCH 23390, in young rats displayed a dentate gyrus-CA1 subfield gradient. The expression was increased in the cell body of dentate gyrus, CA4 and CA3 subfield of old rats compared to younger cohorts, as well as in the neuropil of dentate gyrus. A decreased density of dopamine D1-like receptors was found in the stratum oriens of CA1 and CA3 subfields. Dopamine D2-like receptors, labelled using [ 3 H]-spiperone as radioligand, were expressed rather homogeneously throughout different subfields of the hippocampus. In old rats, the density of dopamine D2-like receptors was decreased in the dentate gyrus, unchanged in the CA4 and CA1 subfields and increased in the CA3 subfield. The above results indicate the occurrence of inhomogeneous changes in the : S 0 0 4 7 -6 3 7 4 ( 0 1 ) 0 0 3 1 7 -7 F. Amenta et al. / Mechanisms of Ageing and De6elopment 122 (2001) 2071-2083 2072 density of dopamine D1-and D2-like receptors in specific portions of hippocampus of old rats. These findings support the hypothesis of an involvement of dopaminergic system in behavioural abnormalities or psychosis occurring in ageing.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13039659" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c6c5e0a718666bee5fea7e415b685716" rel="nofollow" data-download="{"attachment_id":45751213,"asset_id":13039659,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45751213/download_file?st=MTc0MDY0ODAyNCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32273955" href="https://unicam.academia.edu/SeyedTayebati">Seyed Khosrow Tayebati</a><script data-card-contents-for-user="32273955" type="text/json">{"id":32273955,"first_name":"Seyed Khosrow","last_name":"Tayebati","domain_name":"unicam","page_name":"SeyedTayebati","display_name":"Seyed Khosrow Tayebati","profile_url":"https://unicam.academia.edu/SeyedTayebati?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13039659">+1</span><div class="hidden js-additional-users-13039659"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/DanieleTomassoni">Daniele Tomassoni</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13039659'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13039659').html(); 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container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_13039659 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="13039659"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13039659; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13039659]").text(description); $(".js-view-count-work_13039659").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_13039659").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="13039659"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6791" rel="nofollow" href="https://www.academia.edu/Documents/in/Aging">Aging</a>, <script data-card-contents-for-ri="6791" type="text/json">{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="57556" rel="nofollow" href="https://www.academia.edu/Documents/in/Hippocampus">Hippocampus</a>, <script data-card-contents-for-ri="57556" type="text/json">{"id":57556,"name":"Hippocampus","url":"https://www.academia.edu/Documents/in/Hippocampus?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="58513" rel="nofollow" href="https://www.academia.edu/Documents/in/Learning_and_Memory-1">Learning and Memory</a><script data-card-contents-for-ri="58513" type="text/json">{"id":58513,"name":"Learning and Memory","url":"https://www.academia.edu/Documents/in/Learning_and_Memory-1?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13039659]'), work: {"id":13039659,"title":"Age-related changes of dopamine receptors in the rat hippocampus: a light microscope autoradiography study","created_at":"2015-06-16T23:56:51.422-07:00","url":"https://www.academia.edu/13039659/Age_related_changes_of_dopamine_receptors_in_the_rat_hippocampus_a_light_microscope_autoradiography_study?f_ri=3091","dom_id":"work_13039659","summary":"Hippocampus is a brain region involved in learning and memory and is particularly sensitive to ageing. It is supplied with a dopaminergic innervation arising from the midbrain, which is part of the mesolimbic dopaminergic pathway. Dysfunction of the dopaminergic mesolimbic system is probably involved in the pathophysiology of psychosis and behavioural disturbances occurring in the elderly. The present study was designed to assess the density and localisation of dopamine D1-and D2-like receptor subtypes in the hippocampus of male Sprague-Dawley rats aged 3 months (young), 12 months (adult) and 24 months (old). Dopamine D1-like receptors, labelled by [ 3 H]-SCH 23390, in young rats displayed a dentate gyrus-CA1 subfield gradient. The expression was increased in the cell body of dentate gyrus, CA4 and CA3 subfield of old rats compared to younger cohorts, as well as in the neuropil of dentate gyrus. A decreased density of dopamine D1-like receptors was found in the stratum oriens of CA1 and CA3 subfields. Dopamine D2-like receptors, labelled using [ 3 H]-spiperone as radioligand, were expressed rather homogeneously throughout different subfields of the hippocampus. In old rats, the density of dopamine D2-like receptors was decreased in the dentate gyrus, unchanged in the CA4 and CA1 subfields and increased in the CA3 subfield. The above results indicate the occurrence of inhomogeneous changes in the : S 0 0 4 7 -6 3 7 4 ( 0 1 ) 0 0 3 1 7 -7 F. Amenta et al. / Mechanisms of Ageing and De6elopment 122 (2001) 2071-2083 2072 density of dopamine D1-and D2-like receptors in specific portions of hippocampus of old rats. These findings support the hypothesis of an involvement of dopaminergic system in behavioural abnormalities or psychosis occurring in ageing.","downloadable_attachments":[{"id":45751213,"asset_id":13039659,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32273955,"first_name":"Seyed Khosrow","last_name":"Tayebati","domain_name":"unicam","page_name":"SeyedTayebati","display_name":"Seyed Khosrow Tayebati","profile_url":"https://unicam.academia.edu/SeyedTayebati?f_ri=3091","photo":"/images/s65_no_pic.png"},{"id":32274706,"first_name":"Daniele","last_name":"Tomassoni","domain_name":"independent","page_name":"DanieleTomassoni","display_name":"Daniele Tomassoni","profile_url":"https://independent.academia.edu/DanieleTomassoni?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging?f_ri=3091","nofollow":true},{"id":57556,"name":"Hippocampus","url":"https://www.academia.edu/Documents/in/Hippocampus?f_ri=3091","nofollow":true},{"id":58513,"name":"Learning and Memory","url":"https://www.academia.edu/Documents/in/Learning_and_Memory-1?f_ri=3091","nofollow":true},{"id":61474,"name":"Brain","url":"https://www.academia.edu/Documents/in/Brain?f_ri=3091"},{"id":140065,"name":"dopamine receptor D4","url":"https://www.academia.edu/Documents/in/dopamine_receptor_D4?f_ri=3091"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=3091"},{"id":246876,"name":"Dentate Gyrus","url":"https://www.academia.edu/Documents/in/Dentate_Gyrus?f_ri=3091"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=3091"},{"id":509439,"name":"Autoradiography","url":"https://www.academia.edu/Documents/in/Autoradiography?f_ri=3091"},{"id":564878,"name":"Body Weight","url":"https://www.academia.edu/Documents/in/Body_Weight?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13877590" data-work_id="13877590" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13877590/Porphyrin_related_photosensitizers_for_cancer_imaging_and_therapeutic_applications">Porphyrin-related photosensitizers for cancer imaging and therapeutic applications</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A photosensitizer is defined as a chemical entity, which upon absorption of light induces a chemical or physical alteration of another chemical entity. Some photosensitizers are utilized therapeutically such as in photodynamic therapy... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13877590" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A photosensitizer is defined as a chemical entity, which upon absorption of light induces a chemical or physical alteration of another chemical entity. Some photosensitizers are utilized therapeutically such as in photodynamic therapy (PDT) and for diagnosis of cancer (fluorescence diagnosis, FD). PDT is approved for several cancer indications and FD has recently been approved for diagnosis of bladder cancer. The photosensitizers used are in most cases based on the porphyrin structure. These photosensitizers generally accumulate in cancer tissues to a higher extent than in the surrounding tissues and their fluorescing properties may be utilized for cancer detection. The photosensitizers may be chemically synthesized or induced endogenously by an intermediate in heme synthesis, 5-aminolevulinic acid (5-ALA) or 5-ALA esters. The therapeutic effect is based on the formation of reactive oxygen species (ROS) upon activation of the photosensitizer by light. Singlet oxygen is assumed to be the most important ROS for the therapeutic outcome. The fluorescing properties of the photosenisitizers can be used to evaluate their intracellular localization and treatment effects. Some photosensitizers localize intracellularly in endocytic vesicles and upon light exposure induce a release of the contents of these vesicles, including externally added macromolecules, into the cytosol. This is the basis for a novel method for macromolecule activation, named photochemical internalization (PCI). PCI has been shown to potentiate the biological activity of a large variety of macromolecules and other molecules that do not readily penetrate the plasma membrane, including type I ribosome-inactivating proteins, immunotoxins, gene-encoding plasmids, adenovirus, peptide-nucleic acids and the chemotherapeutic drug bleomycin. The background and present status of PDT, FD and PCI are reviewed.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13877590" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2370cad6ff3f34e9edc8af6fdbc61faf" rel="nofollow" data-download="{"attachment_id":44847408,"asset_id":13877590,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44847408/download_file?st=MTc0MDY0ODAyNCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32957405" href="https://independent.academia.edu/LinaPrasmickaite">Lina Prasmickaite</a><script data-card-contents-for-user="32957405" type="text/json">{"id":32957405,"first_name":"Lina","last_name":"Prasmickaite","domain_name":"independent","page_name":"LinaPrasmickaite","display_name":"Lina Prasmickaite","profile_url":"https://independent.academia.edu/LinaPrasmickaite?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_13877590 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13877590"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13877590, container: ".js-paper-rank-work_13877590", }); 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Some photosensitizers are utilized therapeutically such as in photodynamic therapy (PDT) and for diagnosis of cancer (fluorescence diagnosis, FD). PDT is approved for several cancer indications and FD has recently been approved for diagnosis of bladder cancer. The photosensitizers used are in most cases based on the porphyrin structure. These photosensitizers generally accumulate in cancer tissues to a higher extent than in the surrounding tissues and their fluorescing properties may be utilized for cancer detection. The photosensitizers may be chemically synthesized or induced endogenously by an intermediate in heme synthesis, 5-aminolevulinic acid (5-ALA) or 5-ALA esters. The therapeutic effect is based on the formation of reactive oxygen species (ROS) upon activation of the photosensitizer by light. Singlet oxygen is assumed to be the most important ROS for the therapeutic outcome. The fluorescing properties of the photosenisitizers can be used to evaluate their intracellular localization and treatment effects. Some photosensitizers localize intracellularly in endocytic vesicles and upon light exposure induce a release of the contents of these vesicles, including externally added macromolecules, into the cytosol. This is the basis for a novel method for macromolecule activation, named photochemical internalization (PCI). PCI has been shown to potentiate the biological activity of a large variety of macromolecules and other molecules that do not readily penetrate the plasma membrane, including type I ribosome-inactivating proteins, immunotoxins, gene-encoding plasmids, adenovirus, peptide-nucleic acids and the chemotherapeutic drug bleomycin. The background and present status of PDT, FD and PCI are reviewed.","downloadable_attachments":[{"id":44847408,"asset_id":13877590,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32957405,"first_name":"Lina","last_name":"Prasmickaite","domain_name":"independent","page_name":"LinaPrasmickaite","display_name":"Lina Prasmickaite","profile_url":"https://independent.academia.edu/LinaPrasmickaite?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=3091","nofollow":true},{"id":23698,"name":"Porphyrins","url":"https://www.academia.edu/Documents/in/Porphyrins?f_ri=3091"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=3091"},{"id":375077,"name":"Photochemotherapy","url":"https://www.academia.edu/Documents/in/Photochemotherapy?f_ri=3091"},{"id":469018,"name":"Neoplasms","url":"https://www.academia.edu/Documents/in/Neoplasms?f_ri=3091"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_31934949" data-work_id="31934949" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/31934949/Synthesis_and_Mesophases_of_Glycerate_Surfactants">Synthesis and Mesophases of Glycerate Surfactants</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">In the quest to rationally design novel mesophase systems the challenge remains to deconvolute the relationship between structure, composition, and function. In the current study, novel glycerol-derived surfactants with high negative... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_31934949" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In the quest to rationally design novel mesophase systems the challenge remains to deconvolute the relationship between structure, composition, and function. In the current study, novel glycerol-derived surfactants with high negative interfacial areas and a preference for inverse phase behavior have been targeted and synthesized. This has been achieved by application of the rule-of-thumb afforded by the critical packing parameter (CPP), namely, that inverse phase behavior is favored by wedge-shaped molecules with relatively small head group versus chain volume. Highly splayed hydrophobes with exaggerated cross section such as oleyl (cis-octadec-9-enyl) hexahydrofarnesyl (3,7,11-trimethyl-dodecyl) and phytanyl (3,7,11,15-tetramethyl-hexadecyl) were particularly successful for this purpose across many variations of head group. The phase behavior of the binary system in water of many of these surfactants is relatively simple. Typically, cubic or inverse hexagonal phases exist at the interface with water with the inverse micellar phases present at lower hydration. The inverse liquid crystalline phases were present for a broad range of temperatures and compositions.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/31934949" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bb69bb263359bc744e92e37c8c72a9e7" rel="nofollow" data-download="{"attachment_id":52213404,"asset_id":31934949,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/52213404/download_file?st=MTc0MDY0ODAyNCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="2319349" href="https://mmu.academia.edu/JamieBooth">Jamie Booth</a><script data-card-contents-for-user="2319349" type="text/json">{"id":2319349,"first_name":"Jamie","last_name":"Booth","domain_name":"mmu","page_name":"JamieBooth","display_name":"Jamie Booth","profile_url":"https://mmu.academia.edu/JamieBooth?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_31934949 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="31934949"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 31934949, container: ".js-paper-rank-work_31934949", }); 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In the current study, novel glycerol-derived surfactants with high negative interfacial areas and a preference for inverse phase behavior have been targeted and synthesized. This has been achieved by application of the rule-of-thumb afforded by the critical packing parameter (CPP), namely, that inverse phase behavior is favored by wedge-shaped molecules with relatively small head group versus chain volume. Highly splayed hydrophobes with exaggerated cross section such as oleyl (cis-octadec-9-enyl) hexahydrofarnesyl (3,7,11-trimethyl-dodecyl) and phytanyl (3,7,11,15-tetramethyl-hexadecyl) were particularly successful for this purpose across many variations of head group. The phase behavior of the binary system in water of many of these surfactants is relatively simple. Typically, cubic or inverse hexagonal phases exist at the interface with water with the inverse micellar phases present at lower hydration. The inverse liquid crystalline phases were present for a broad range of temperatures and compositions.","downloadable_attachments":[{"id":52213404,"asset_id":31934949,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":2319349,"first_name":"Jamie","last_name":"Booth","domain_name":"mmu","page_name":"JamieBooth","display_name":"Jamie Booth","profile_url":"https://mmu.academia.edu/JamieBooth?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=3091","nofollow":true},{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":5020,"name":"Liquid Crystals","url":"https://www.academia.edu/Documents/in/Liquid_Crystals?f_ri=3091","nofollow":true},{"id":70047,"name":"Micelles","url":"https://www.academia.edu/Documents/in/Micelles?f_ri=3091"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=3091"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=3091"},{"id":161176,"name":"The","url":"https://www.academia.edu/Documents/in/The?f_ri=3091"},{"id":173963,"name":"Phase transition","url":"https://www.academia.edu/Documents/in/Phase_transition?f_ri=3091"},{"id":205584,"name":"Solubility","url":"https://www.academia.edu/Documents/in/Solubility?f_ri=3091"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=3091"},{"id":283379,"name":"Glycerol","url":"https://www.academia.edu/Documents/in/Glycerol?f_ri=3091"},{"id":564340,"name":"Surface Active Agents","url":"https://www.academia.edu/Documents/in/Surface_Active_Agents?f_ri=3091"},{"id":725266,"name":"Oleic Acid","url":"https://www.academia.edu/Documents/in/Oleic_Acid?f_ri=3091"},{"id":889639,"name":"Fatty Alcohols","url":"https://www.academia.edu/Documents/in/Fatty_Alcohols?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_707851" data-work_id="707851" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/707851/Peptide_Nanotube_Nematic_Phase">Peptide Nanotube Nematic Phase</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The self-assembly of the trifluoroacetate salt of the short peptide (ala) 6 -lys (A 6 K) in water has been investigated by cryo-transmission electron microscopy and small-angle X-ray scattering. For concentrations below ca. 12%, the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_707851" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The self-assembly of the trifluoroacetate salt of the short peptide (ala) 6 -lys (A 6 K) in water has been investigated by cryo-transmission electron microscopy and small-angle X-ray scattering. For concentrations below ca. 12%, the peptide does not self-assemble but forms a molecularly dispersed solution. Above this critical concentration, however, A 6 K self-assembles into several-micrometer-long hollow nanotubes with a monodisperse crosssectional radius of 26 nm. Because the peptides carry a positive charge, the nanotubes are charge-stabilized. Because of the very large aspect ratio, the tubes form an ordered phase that presumably is nematic. (16) Knaapila, M.; Svensson, C.; Barauskas, J.; Zackrisson, M.; Nielsen, S. S.; Toft, K. N.; Vestergaard, B.; Arleth, L.; Olsson, U.; Pedersen, J. S.; Cerenius, Y.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/707851" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c1b4c5f48be4b32e11384d79613122b9" rel="nofollow" data-download="{"attachment_id":51279989,"asset_id":707851,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/51279989/download_file?st=MTc0MDY0ODAyNCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="522377" href="https://lu.academia.edu/IremNasir">Irem Nasir</a><script data-card-contents-for-user="522377" type="text/json">{"id":522377,"first_name":"Irem","last_name":"Nasir","domain_name":"lu","page_name":"IremNasir","display_name":"Irem Nasir","profile_url":"https://lu.academia.edu/IremNasir?f_ri=3091","photo":"https://0.academia-photos.com/522377/184162/227702/s65_irem.nasir.jpg"}</script></span></span></li><li class="js-paper-rank-work_707851 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="707851"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 707851, container: ".js-paper-rank-work_707851", }); });</script></li><li class="js-percentile-work_707851 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 707851; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_707851"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_707851 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="707851"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 707851; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=707851]").text(description); $(".js-view-count-work_707851").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_707851").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="707851"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2215" rel="nofollow" href="https://www.academia.edu/Documents/in/Water">Water</a>, <script data-card-contents-for-ri="2215" type="text/json">{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="10636" rel="nofollow" href="https://www.academia.edu/Documents/in/Small_Angle_X_Ray_Scattering">Small Angle X Ray Scattering</a>, <script data-card-contents-for-ri="10636" type="text/json">{"id":10636,"name":"Small Angle X Ray Scattering","url":"https://www.academia.edu/Documents/in/Small_Angle_X_Ray_Scattering?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="11073" rel="nofollow" href="https://www.academia.edu/Documents/in/Self_Assembly">Self Assembly</a><script data-card-contents-for-ri="11073" type="text/json">{"id":11073,"name":"Self Assembly","url":"https://www.academia.edu/Documents/in/Self_Assembly?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=707851]'), work: {"id":707851,"title":"Peptide Nanotube Nematic Phase","created_at":"2011-06-30T05:02:54.839-07:00","url":"https://www.academia.edu/707851/Peptide_Nanotube_Nematic_Phase?f_ri=3091","dom_id":"work_707851","summary":"The self-assembly of the trifluoroacetate salt of the short peptide (ala) 6 -lys (A 6 K) in water has been investigated by cryo-transmission electron microscopy and small-angle X-ray scattering. For concentrations below ca. 12%, the peptide does not self-assemble but forms a molecularly dispersed solution. Above this critical concentration, however, A 6 K self-assembles into several-micrometer-long hollow nanotubes with a monodisperse crosssectional radius of 26 nm. Because the peptides carry a positive charge, the nanotubes are charge-stabilized. Because of the very large aspect ratio, the tubes form an ordered phase that presumably is nematic. (16) Knaapila, M.; Svensson, C.; Barauskas, J.; Zackrisson, M.; Nielsen, S. S.; Toft, K. N.; Vestergaard, B.; Arleth, L.; Olsson, U.; Pedersen, J. S.; Cerenius, Y.","downloadable_attachments":[{"id":51279989,"asset_id":707851,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":522377,"first_name":"Irem","last_name":"Nasir","domain_name":"lu","page_name":"IremNasir","display_name":"Irem Nasir","profile_url":"https://lu.academia.edu/IremNasir?f_ri=3091","photo":"https://0.academia-photos.com/522377/184162/227702/s65_irem.nasir.jpg"}],"research_interests":[{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":10636,"name":"Small Angle X Ray Scattering","url":"https://www.academia.edu/Documents/in/Small_Angle_X_Ray_Scattering?f_ri=3091","nofollow":true},{"id":11073,"name":"Self Assembly","url":"https://www.academia.edu/Documents/in/Self_Assembly?f_ri=3091","nofollow":true},{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy?f_ri=3091"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=3091"},{"id":35199,"name":"Peptide Self-Assembly","url":"https://www.academia.edu/Documents/in/Peptide_Self-Assembly?f_ri=3091"},{"id":56001,"name":"X Rays","url":"https://www.academia.edu/Documents/in/X_Rays?f_ri=3091"},{"id":77190,"name":"SAXS","url":"https://www.academia.edu/Documents/in/SAXS?f_ri=3091"},{"id":111748,"name":"Nanotubes","url":"https://www.academia.edu/Documents/in/Nanotubes?f_ri=3091"},{"id":151086,"name":"Peptides","url":"https://www.academia.edu/Documents/in/Peptides?f_ri=3091"},{"id":199325,"name":"Peptide Nanotubes","url":"https://www.academia.edu/Documents/in/Peptide_Nanotubes?f_ri=3091"},{"id":440924,"name":"Surface Properties","url":"https://www.academia.edu/Documents/in/Surface_Properties?f_ri=3091"},{"id":743666,"name":"Langmuir","url":"https://www.academia.edu/Documents/in/Langmuir?f_ri=3091"},{"id":958106,"name":"Salts","url":"https://www.academia.edu/Documents/in/Salts?f_ri=3091"},{"id":2571694,"name":"Micrometer","url":"https://www.academia.edu/Documents/in/Micrometer?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3803153" data-work_id="3803153" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3803153/Determination_of_mechanical_properties_of_layered_materials_with_vector_contrast_scanning_acoustic_microscopy_by_polar_diagram_image_representation">Determination of mechanical properties of layered materials with vector-contrast scanning acoustic microscopy by polar diagram image representation</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Microscopic objects including living cells on a planar substrate are investigated in bio-medical applications of scanning acoustic microscopy. Beside of the observation of lateral structures, the determination of sample properties such as... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_3803153" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Microscopic objects including living cells on a planar substrate are investigated in bio-medical applications of scanning acoustic microscopy. Beside of the observation of lateral structures, the determination of sample properties such as density, sound velocity, and attenuation is desired, from which elastic properties can be derived. This can be achieved with the aid of the acoustic phase and magnitude contrast represented in a polar plot. For homogeneous and sufficiently planar objects the contrast in magnitude and phase is a function of the properties of the substrate and the coupling fluid, which both can easily be determined, and of the mechanical properties of the sample under observation. For observation in reflection and variable thickness of the sample the signal will depend on the actual thickness. This signature of the object can be fitted based on a conventional ray model for the sound propagating in the coupling medium and the sample. The model includes also the refraction and reflection at all interfaces between transducer, lens material, coupling fluid, object, and substrate. The method is demonstrated for a chitosan film deposited on a glass substrate. The scheme presented here is capable to reach a resolution of about and even below 1% for relevant quantities in applications involving imaging at 1.2 GHz in aqueous coupling fluids.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3803153" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c182be0e889bb07e4527a5669f7539af" rel="nofollow" data-download="{"attachment_id":31465672,"asset_id":3803153,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/31465672/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4676543" href="https://google.academia.edu/EsamEldinTAhmedMohamed">Esam Eldin T Ahmed Mohamed</a><script data-card-contents-for-user="4676543" type="text/json">{"id":4676543,"first_name":"Esam Eldin","last_name":"Ahmed Mohamed","domain_name":"google","page_name":"EsamEldinTAhmedMohamed","display_name":"Esam Eldin T Ahmed Mohamed","profile_url":"https://google.academia.edu/EsamEldinTAhmedMohamed?f_ri=3091","photo":"https://0.academia-photos.com/4676543/2912742/8126652/s65_esam_eldin.ahmed_mohamed.png"}</script></span></span></li><li class="js-paper-rank-work_3803153 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="3803153"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 3803153, container: ".js-paper-rank-work_3803153", }); });</script></li><li class="js-percentile-work_3803153 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 3803153; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_3803153"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_3803153 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="3803153"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 3803153; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=3803153]").text(description); $(".js-view-count-work_3803153").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_3803153").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="3803153"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">5</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="29169" rel="nofollow" href="https://www.academia.edu/Documents/in/Reflection">Reflection</a>, <script data-card-contents-for-ri="29169" type="text/json">{"id":29169,"name":"Reflection","url":"https://www.academia.edu/Documents/in/Reflection?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="67357" rel="nofollow" href="https://www.academia.edu/Documents/in/Transducers">Transducers</a>, <script data-card-contents-for-ri="67357" type="text/json">{"id":67357,"name":"Transducers","url":"https://www.academia.edu/Documents/in/Transducers?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="176632" rel="nofollow" href="https://www.academia.edu/Documents/in/Interfaces">Interfaces</a><script data-card-contents-for-ri="176632" type="text/json">{"id":176632,"name":"Interfaces","url":"https://www.academia.edu/Documents/in/Interfaces?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=3803153]'), work: {"id":3803153,"title":"Determination of mechanical properties of layered materials with vector-contrast scanning acoustic microscopy by polar diagram image representation","created_at":"2013-06-27T05:27:32.409-07:00","url":"https://www.academia.edu/3803153/Determination_of_mechanical_properties_of_layered_materials_with_vector_contrast_scanning_acoustic_microscopy_by_polar_diagram_image_representation?f_ri=3091","dom_id":"work_3803153","summary":"Microscopic objects including living cells on a planar substrate are investigated in bio-medical applications of scanning acoustic microscopy. Beside of the observation of lateral structures, the determination of sample properties such as density, sound velocity, and attenuation is desired, from which elastic properties can be derived. This can be achieved with the aid of the acoustic phase and magnitude contrast represented in a polar plot. For homogeneous and sufficiently planar objects the contrast in magnitude and phase is a function of the properties of the substrate and the coupling fluid, which both can easily be determined, and of the mechanical properties of the sample under observation. For observation in reflection and variable thickness of the sample the signal will depend on the actual thickness. This signature of the object can be fitted based on a conventional ray model for the sound propagating in the coupling medium and the sample. The model includes also the refraction and reflection at all interfaces between transducer, lens material, coupling fluid, object, and substrate. The method is demonstrated for a chitosan film deposited on a glass substrate. The scheme presented here is capable to reach a resolution of about and even below 1% for relevant quantities in applications involving imaging at 1.2 GHz in aqueous coupling fluids.","downloadable_attachments":[{"id":31465672,"asset_id":3803153,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4676543,"first_name":"Esam Eldin","last_name":"Ahmed Mohamed","domain_name":"google","page_name":"EsamEldinTAhmedMohamed","display_name":"Esam Eldin T Ahmed Mohamed","profile_url":"https://google.academia.edu/EsamEldinTAhmedMohamed?f_ri=3091","photo":"https://0.academia-photos.com/4676543/2912742/8126652/s65_esam_eldin.ahmed_mohamed.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":29169,"name":"Reflection","url":"https://www.academia.edu/Documents/in/Reflection?f_ri=3091","nofollow":true},{"id":67357,"name":"Transducers","url":"https://www.academia.edu/Documents/in/Transducers?f_ri=3091","nofollow":true},{"id":176632,"name":"Interfaces","url":"https://www.academia.edu/Documents/in/Interfaces?f_ri=3091","nofollow":true},{"id":428971,"name":"Refraction","url":"https://www.academia.edu/Documents/in/Refraction?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_28911721" data-work_id="28911721" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/28911721/Use_of_electric_fields_to_monitor_the_dynamical_aspect_of_cell_behavior_in_tissue_culture">Use of electric fields to monitor the dynamical aspect of cell behavior in tissue culture</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Abstrat-Mammalian cells can be cultured and therefore studied in vitro. Normally, the cells' morphology and other static properties are observed with the aid of a light microscope. A method is described here that allows observation of the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_28911721" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Abstrat-Mammalian cells can be cultured and therefore studied in vitro. Normally, the cells' morphology and other static properties are observed with the aid of a light microscope. A method is described here that allows observation of the dynamical aspects of cultured cells. Mammalian fibroblasts are cultured in polystyrene dishes that contain evaporated gold electrodes. As the cells attach to the electrodes, their presence and their motion is directly reflected in the -measured impedance.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/28911721" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="26a453185ff7f7f6f1f4a18059fecc74" rel="nofollow" data-download="{"attachment_id":49345591,"asset_id":28911721,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49345591/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="54464610" href="https://independent.academia.edu/CharlesKeese">Charles Keese</a><script data-card-contents-for-user="54464610" type="text/json">{"id":54464610,"first_name":"Charles","last_name":"Keese","domain_name":"independent","page_name":"CharlesKeese","display_name":"Charles Keese","profile_url":"https://independent.academia.edu/CharlesKeese?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_28911721 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="28911721"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 28911721, container: ".js-paper-rank-work_28911721", }); });</script></li><li class="js-percentile-work_28911721 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 28911721; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_28911721"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_28911721 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="28911721"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 28911721; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=28911721]").text(description); $(".js-view-count-work_28911721").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_28911721").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="28911721"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1131" rel="nofollow" href="https://www.academia.edu/Documents/in/Biomedical_Engineering">Biomedical Engineering</a>, <script data-card-contents-for-ri="1131" type="text/json">{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4748" rel="nofollow" href="https://www.academia.edu/Documents/in/Electrochemistry">Electrochemistry</a>, <script data-card-contents-for-ri="4748" type="text/json">{"id":4748,"name":"Electrochemistry","url":"https://www.academia.edu/Documents/in/Electrochemistry?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="10055" rel="nofollow" href="https://www.academia.edu/Documents/in/Cell_Adhesion">Cell Adhesion</a><script data-card-contents-for-ri="10055" type="text/json">{"id":10055,"name":"Cell Adhesion","url":"https://www.academia.edu/Documents/in/Cell_Adhesion?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=28911721]'), work: {"id":28911721,"title":"Use of electric fields to monitor the dynamical aspect of cell behavior in tissue culture","created_at":"2016-10-04T07:18:51.666-07:00","url":"https://www.academia.edu/28911721/Use_of_electric_fields_to_monitor_the_dynamical_aspect_of_cell_behavior_in_tissue_culture?f_ri=3091","dom_id":"work_28911721","summary":"Abstrat-Mammalian cells can be cultured and therefore studied in vitro. Normally, the cells' morphology and other static properties are observed with the aid of a light microscope. A method is described here that allows observation of the dynamical aspects of cultured cells. Mammalian fibroblasts are cultured in polystyrene dishes that contain evaporated gold electrodes. As the cells attach to the electrodes, their presence and their motion is directly reflected in the -measured impedance.","downloadable_attachments":[{"id":49345591,"asset_id":28911721,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":54464610,"first_name":"Charles","last_name":"Keese","domain_name":"independent","page_name":"CharlesKeese","display_name":"Charles Keese","profile_url":"https://independent.academia.edu/CharlesKeese?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":4748,"name":"Electrochemistry","url":"https://www.academia.edu/Documents/in/Electrochemistry?f_ri=3091","nofollow":true},{"id":10055,"name":"Cell Adhesion","url":"https://www.academia.edu/Documents/in/Cell_Adhesion?f_ri=3091","nofollow":true},{"id":10866,"name":"Morphology","url":"https://www.academia.edu/Documents/in/Morphology?f_ri=3091"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=3091"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro?f_ri=3091"},{"id":66089,"name":"Tissue culture","url":"https://www.academia.edu/Documents/in/Tissue_culture?f_ri=3091"},{"id":184907,"name":"Microelectrodes","url":"https://www.academia.edu/Documents/in/Microelectrodes?f_ri=3091"},{"id":846015,"name":"Electrodes","url":"https://www.academia.edu/Documents/in/Electrodes?f_ri=3091"},{"id":846581,"name":"Cell communication","url":"https://www.academia.edu/Documents/in/Cell_communication?f_ri=3091"},{"id":1130559,"name":"Electric Field","url":"https://www.academia.edu/Documents/in/Electric_Field?f_ri=3091"},{"id":1237788,"name":"Electrical And Electronic Engineering","url":"https://www.academia.edu/Documents/in/Electrical_And_Electronic_Engineering?f_ri=3091"},{"id":2533047,"name":"fibroblasts","url":"https://www.academia.edu/Documents/in/fibroblasts?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_25241330" data-work_id="25241330" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/25241330/The_Tension_Mounts_Mechanics_Meets_Morphogenesis_and_Malignancy">The Tension Mounts: Mechanics Meets Morphogenesis and Malignancy</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm 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class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32576680" href="https://ucsf.academia.edu/ValerieWeaver">Valerie Weaver</a><script data-card-contents-for-user="32576680" type="text/json">{"id":32576680,"first_name":"Valerie","last_name":"Weaver","domain_name":"ucsf","page_name":"ValerieWeaver","display_name":"Valerie Weaver","profile_url":"https://ucsf.academia.edu/ValerieWeaver?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_25241330 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="25241330"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 25241330, container: ".js-paper-rank-work_25241330", }); });</script></li><li class="js-percentile-work_25241330 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 25241330; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_25241330"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_25241330 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="25241330"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 25241330; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=25241330]").text(description); $(".js-view-count-work_25241330").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_25241330").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="25241330"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="512" rel="nofollow" href="https://www.academia.edu/Documents/in/Mechanics">Mechanics</a>, <script data-card-contents-for-ri="512" type="text/json">{"id":512,"name":"Mechanics","url":"https://www.academia.edu/Documents/in/Mechanics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="24124" rel="nofollow" href="https://www.academia.edu/Documents/in/Morphogenesis">Morphogenesis</a>, <script data-card-contents-for-ri="24124" type="text/json">{"id":24124,"name":"Morphogenesis","url":"https://www.academia.edu/Documents/in/Morphogenesis?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="29149" rel="nofollow" href="https://www.academia.edu/Documents/in/Extracellular_Matrix">Extracellular Matrix</a><script data-card-contents-for-ri="29149" type="text/json">{"id":29149,"name":"Extracellular Matrix","url":"https://www.academia.edu/Documents/in/Extracellular_Matrix?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=25241330]'), work: {"id":25241330,"title":"The Tension Mounts: Mechanics Meets Morphogenesis and Malignancy","created_at":"2016-05-11T11:21:51.833-07:00","url":"https://www.academia.edu/25241330/The_Tension_Mounts_Mechanics_Meets_Morphogenesis_and_Malignancy?f_ri=3091","dom_id":"work_25241330","summary":null,"downloadable_attachments":[{"id":45547695,"asset_id":25241330,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32576680,"first_name":"Valerie","last_name":"Weaver","domain_name":"ucsf","page_name":"ValerieWeaver","display_name":"Valerie Weaver","profile_url":"https://ucsf.academia.edu/ValerieWeaver?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":512,"name":"Mechanics","url":"https://www.academia.edu/Documents/in/Mechanics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":24124,"name":"Morphogenesis","url":"https://www.academia.edu/Documents/in/Morphogenesis?f_ri=3091","nofollow":true},{"id":29149,"name":"Extracellular Matrix","url":"https://www.academia.edu/Documents/in/Extracellular_Matrix?f_ri=3091","nofollow":true},{"id":130971,"name":"Mechanotransduction","url":"https://www.academia.edu/Documents/in/Mechanotransduction?f_ri=3091"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=3091"},{"id":379889,"name":"Homeostasis","url":"https://www.academia.edu/Documents/in/Homeostasis?f_ri=3091"},{"id":434050,"name":"Mammary gland","url":"https://www.academia.edu/Documents/in/Mammary_gland?f_ri=3091"},{"id":469018,"name":"Neoplasms","url":"https://www.academia.edu/Documents/in/Neoplasms?f_ri=3091"},{"id":497482,"name":"Force","url":"https://www.academia.edu/Documents/in/Force?f_ri=3091"},{"id":504035,"name":"Three Dimensional","url":"https://www.academia.edu/Documents/in/Three_Dimensional?f_ri=3091"},{"id":951407,"name":"Malignant Transformation","url":"https://www.academia.edu/Documents/in/Malignant_Transformation?f_ri=3091"},{"id":984451,"name":"Treatment Response","url":"https://www.academia.edu/Documents/in/Treatment_Response?f_ri=3091"},{"id":1178162,"name":"Tumor Growth","url":"https://www.academia.edu/Documents/in/Tumor_Growth?f_ri=3091"},{"id":1362702,"name":"Traction","url":"https://www.academia.edu/Documents/in/Traction?f_ri=3091"},{"id":1670920,"name":"Integrin","url":"https://www.academia.edu/Documents/in/Integrin?f_ri=3091"},{"id":1902100,"name":"Biomechanical Phenomena","url":"https://www.academia.edu/Documents/in/Biomechanical_Phenomena?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24477937" data-work_id="24477937" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24477937/Monitoring_toxic_microalgae_Ostreopsis_dinoflagellate_species_in_coastal_waters_of_the_Mediterranean_Sea_using_molecular_PCR_based_assay_combined_with_light_microscopy">Monitoring toxic microalgae Ostreopsis (dinoflagellate) species in coastal waters of the Mediterranean Sea using molecular PCR-based assay combined with light microscopy</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A molecular PCR-based assay was developed and applied to macrophyte and seawater samples containing mixed microphytobenthic and phytoplanktonic assemblages, respectively, in order to detect toxic Ostreopsis species in Mediterranean Sea.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24477937" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A molecular PCR-based assay was developed and applied to macrophyte and seawater samples containing mixed microphytobenthic and phytoplanktonic assemblages, respectively, in order to detect toxic Ostreopsis species in Mediterranean Sea. The specificity and sensitivity of the molecular PCR assay were assessed with both plasmidic and genomic DNA of the target genus or species using taxon-specific primers in the presence of background macrophyte DNA. The PCR molecular technique allowed rapid detection of the Ostreopsis cells, even at abundances undetectable within the resolution limit of the microscopy technique. Species-specific identification of Ostreopsis was determined only by PCR-based assay, due to the inherent difficulty of morphological identification in field samples. In the monitoring of the toxic Ostreopsis blooms PCR-based methods proved to be effective tools complementary to microscopy for rapid and specific detection of Ostreopsis and other toxic dinoflagellates in marine coastal environments.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24477937" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="42e897827d59be57d5d77b92ed3ad86b" rel="nofollow" data-download="{"attachment_id":44812063,"asset_id":24477937,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44812063/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="47180687" href="https://univpm.academia.edu/StefanoAccoroni">Stefano Accoroni</a><script data-card-contents-for-user="47180687" type="text/json">{"id":47180687,"first_name":"Stefano","last_name":"Accoroni","domain_name":"univpm","page_name":"StefanoAccoroni","display_name":"Stefano Accoroni","profile_url":"https://univpm.academia.edu/StefanoAccoroni?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24477937 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24477937"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24477937, container: ".js-paper-rank-work_24477937", }); 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$(".js-view-count[data-work-id=24477937]").text(description); $(".js-view-count-work_24477937").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24477937").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24477937"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">23</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="261" rel="nofollow" href="https://www.academia.edu/Documents/in/Geography">Geography</a>, <script data-card-contents-for-ri="261" type="text/json">{"id":261,"name":"Geography","url":"https://www.academia.edu/Documents/in/Geography?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4552" rel="nofollow" href="https://www.academia.edu/Documents/in/Ecotoxicology">Ecotoxicology</a>, <script data-card-contents-for-ri="4552" type="text/json">{"id":4552,"name":"Ecotoxicology","url":"https://www.academia.edu/Documents/in/Ecotoxicology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9513" rel="nofollow" href="https://www.academia.edu/Documents/in/MARINE_POLLUTION">MARINE POLLUTION</a><script data-card-contents-for-ri="9513" type="text/json">{"id":9513,"name":"MARINE POLLUTION","url":"https://www.academia.edu/Documents/in/MARINE_POLLUTION?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24477937]'), work: {"id":24477937,"title":"Monitoring toxic microalgae Ostreopsis (dinoflagellate) species in coastal waters of the Mediterranean Sea using molecular PCR-based assay combined with light microscopy","created_at":"2016-04-17T00:10:43.997-07:00","url":"https://www.academia.edu/24477937/Monitoring_toxic_microalgae_Ostreopsis_dinoflagellate_species_in_coastal_waters_of_the_Mediterranean_Sea_using_molecular_PCR_based_assay_combined_with_light_microscopy?f_ri=3091","dom_id":"work_24477937","summary":"A molecular PCR-based assay was developed and applied to macrophyte and seawater samples containing mixed microphytobenthic and phytoplanktonic assemblages, respectively, in order to detect toxic Ostreopsis species in Mediterranean Sea. The specificity and sensitivity of the molecular PCR assay were assessed with both plasmidic and genomic DNA of the target genus or species using taxon-specific primers in the presence of background macrophyte DNA. The PCR molecular technique allowed rapid detection of the Ostreopsis cells, even at abundances undetectable within the resolution limit of the microscopy technique. Species-specific identification of Ostreopsis was determined only by PCR-based assay, due to the inherent difficulty of morphological identification in field samples. In the monitoring of the toxic Ostreopsis blooms PCR-based methods proved to be effective tools complementary to microscopy for rapid and specific detection of Ostreopsis and other toxic dinoflagellates in marine coastal environments.","downloadable_attachments":[{"id":44812063,"asset_id":24477937,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":47180687,"first_name":"Stefano","last_name":"Accoroni","domain_name":"univpm","page_name":"StefanoAccoroni","display_name":"Stefano Accoroni","profile_url":"https://univpm.academia.edu/StefanoAccoroni?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":261,"name":"Geography","url":"https://www.academia.edu/Documents/in/Geography?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":4552,"name":"Ecotoxicology","url":"https://www.academia.edu/Documents/in/Ecotoxicology?f_ri=3091","nofollow":true},{"id":9513,"name":"MARINE POLLUTION","url":"https://www.academia.edu/Documents/in/MARINE_POLLUTION?f_ri=3091","nofollow":true},{"id":21724,"name":"Water Pollution","url":"https://www.academia.edu/Documents/in/Water_Pollution?f_ri=3091"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=3091"},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=3091"},{"id":50243,"name":"Mediterranean Sea","url":"https://www.academia.edu/Documents/in/Mediterranean_Sea?f_ri=3091"},{"id":51791,"name":"PCR","url":"https://www.academia.edu/Documents/in/PCR?f_ri=3091"},{"id":67405,"name":"Toxicity","url":"https://www.academia.edu/Documents/in/Toxicity?f_ri=3091"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=3091"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=3091"},{"id":176486,"name":"Genome","url":"https://www.academia.edu/Documents/in/Genome?f_ri=3091"},{"id":184467,"name":"Seawater","url":"https://www.academia.edu/Documents/in/Seawater?f_ri=3091"},{"id":190363,"name":"Plasmids","url":"https://www.academia.edu/Documents/in/Plasmids?f_ri=3091"},{"id":371424,"name":"Genomic DNA","url":"https://www.academia.edu/Documents/in/Genomic_DNA?f_ri=3091"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=3091"},{"id":477461,"name":"Coastal Zone","url":"https://www.academia.edu/Documents/in/Coastal_Zone?f_ri=3091"},{"id":571946,"name":"Coastal waters","url":"https://www.academia.edu/Documents/in/Coastal_waters?f_ri=3091"},{"id":649451,"name":"Seasons","url":"https://www.academia.edu/Documents/in/Seasons?f_ri=3091"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity?f_ri=3091"},{"id":885893,"name":"Light microscopy","url":"https://www.academia.edu/Documents/in/Light_microscopy?f_ri=3091"},{"id":2313637,"name":"Coastal Area","url":"https://www.academia.edu/Documents/in/Coastal_Area?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15631652" data-work_id="15631652" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15631652/Distribution_of_ghrelin_peptide_in_the_gastrointestinal_tract_of_stomachless_and_stomach_containing_teleosts">Distribution of ghrelin peptide in the gastrointestinal tract of stomachless and stomach-containing teleosts</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The occurrence and localization of ghrelin peptide in the gastrointestinal tract of Carassius auratus and Dicentrarchus labrax, two fish species that exhibit different feeding behavior, different habitats, and different anatomical... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15631652" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The occurrence and localization of ghrelin peptide in the gastrointestinal tract of Carassius auratus and Dicentrarchus labrax, two fish species that exhibit different feeding behavior, different habitats, and different anatomical organizations of the gastroenteric tract, were examined by immunohistochemical methods and western blotting analysis. All of the gastrointestinal segments studied displayed immunohistochemical localizations of ghrelin peptide. Numerous single or clustered immunoreactive cells were found along the gastric folds, particularly in the pyloric region of Dicentrarcus labrax, whereas scattered ghrelin immunoreactive cells were observed in the intestinal epithelium of both fish species. Double immunolabeling PGP 9.5/ghrelin demonstrated the localization of ghrelin peptide also in nerve fibers and neuronal cells of the submucosal and myenteric plexuses, often in association with vascular structures. Western blotting analysis confirmed the presence of ghrelin peptide in the gatrointestinal tract of both species studied, whose molecular weight was similar to that of the corresponding mammalian prepro-ghrelin. The findings could support the hypothesis that this peptide is an important appetite regulator in fish and could confirm the presence of the ghrelin peptide, starting from its precursor proteins, in the gastrointestinal tract of the goldfish and the sea bass. Microsc. Res. Tech. 00:000-000, 2009. V</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15631652" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="10aa89670464e9002ac8c2fd47263716" rel="nofollow" data-download="{"attachment_id":43014614,"asset_id":15631652,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43014614/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34809629" href="https://independent.academia.edu/FiniziaRusso">Finizia Russo</a><script data-card-contents-for-user="34809629" type="text/json">{"id":34809629,"first_name":"Finizia","last_name":"Russo","domain_name":"independent","page_name":"FiniziaRusso","display_name":"Finizia Russo","profile_url":"https://independent.academia.edu/FiniziaRusso?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_15631652 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="15631652"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 15631652, container: ".js-paper-rank-work_15631652", }); 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$(".js-view-count[data-work-id=15631652]").text(description); $(".js-view-count-work_15631652").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_15631652").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="15631652"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="56" rel="nofollow" href="https://www.academia.edu/Documents/in/Materials_Engineering">Materials Engineering</a>, <script data-card-contents-for-ri="56" type="text/json">{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37834" rel="nofollow" href="https://www.academia.edu/Documents/in/Western_blotting">Western blotting</a><script data-card-contents-for-ri="37834" type="text/json">{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=15631652]'), work: {"id":15631652,"title":"Distribution of ghrelin peptide in the gastrointestinal tract of stomachless and stomach-containing teleosts","created_at":"2015-09-12T12:27:21.613-07:00","url":"https://www.academia.edu/15631652/Distribution_of_ghrelin_peptide_in_the_gastrointestinal_tract_of_stomachless_and_stomach_containing_teleosts?f_ri=3091","dom_id":"work_15631652","summary":"The occurrence and localization of ghrelin peptide in the gastrointestinal tract of Carassius auratus and Dicentrarchus labrax, two fish species that exhibit different feeding behavior, different habitats, and different anatomical organizations of the gastroenteric tract, were examined by immunohistochemical methods and western blotting analysis. All of the gastrointestinal segments studied displayed immunohistochemical localizations of ghrelin peptide. Numerous single or clustered immunoreactive cells were found along the gastric folds, particularly in the pyloric region of Dicentrarcus labrax, whereas scattered ghrelin immunoreactive cells were observed in the intestinal epithelium of both fish species. Double immunolabeling PGP 9.5/ghrelin demonstrated the localization of ghrelin peptide also in nerve fibers and neuronal cells of the submucosal and myenteric plexuses, often in association with vascular structures. Western blotting analysis confirmed the presence of ghrelin peptide in the gatrointestinal tract of both species studied, whose molecular weight was similar to that of the corresponding mammalian prepro-ghrelin. The findings could support the hypothesis that this peptide is an important appetite regulator in fish and could confirm the presence of the ghrelin peptide, starting from its precursor proteins, in the gastrointestinal tract of the goldfish and the sea bass. Microsc. Res. Tech. 00:000-000, 2009. V","downloadable_attachments":[{"id":43014614,"asset_id":15631652,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34809629,"first_name":"Finizia","last_name":"Russo","domain_name":"independent","page_name":"FiniziaRusso","display_name":"Finizia Russo","profile_url":"https://independent.academia.edu/FiniziaRusso?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=3091","nofollow":true},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=3091","nofollow":true},{"id":41783,"name":"Ghrelin","url":"https://www.academia.edu/Documents/in/Ghrelin?f_ri=3091"},{"id":71471,"name":"Intestinal Mucosa","url":"https://www.academia.edu/Documents/in/Intestinal_Mucosa?f_ri=3091"},{"id":193974,"name":"Neurons","url":"https://www.academia.edu/Documents/in/Neurons?f_ri=3091"},{"id":302497,"name":"Goldfish","url":"https://www.academia.edu/Documents/in/Goldfish?f_ri=3091"},{"id":537759,"name":"Gastrointestinal Tract","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Tract?f_ri=3091"},{"id":695018,"name":"Molecular weight","url":"https://www.academia.edu/Documents/in/Molecular_weight?f_ri=3091"},{"id":735530,"name":"Bass","url":"https://www.academia.edu/Documents/in/Bass?f_ri=3091"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_66347396" data-work_id="66347396" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" rel="nofollow" href="https://www.academia.edu/66347396/Two_still_life_paintings_by_Jan_van_Huysum_an_examination_of_painting_technique_in_relation_to_documentary_and_technical_evidence">Two still-life paintings by Jan van Huysum: an examination of painting technique in relation to documentary and technical evidence</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Two still-life paintings were scientifically examined in order to gain insights about Dutch painter Jan van Huysum&#39;s (1682-1749) purportedly secretive paintings techniques, comparing the actual works to information obtained in the few... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_66347396" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Two still-life paintings were scientifically examined in order to gain insights about Dutch painter Jan van Huysum&#39;s (1682-1749) purportedly secretive paintings techniques, comparing the actual works to information obtained in the few available historical technical sources. Surface investigations included macroscopic and microscopic examination, infrared reflectography and x-radiography, while material characterization of the ground, organic and inorganic pigments and binding media was carried out with a range of non-destructive and destructive analytical techniques. The belief that the two works were an original pair of pendant paintings was disproved due to substantial technical differences in the wood support, ground layers and use of yellow pigment. It was also found that Van Huysum&#39;s use of newly developed pigments (Prussian blue, Naples yellow) had lead to changes in his painting technique, which no longer corresponded to those described in the period&#39;s manuals, an...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/66347396" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="51706210" href="https://independent.academia.edu/WallertArie">Arie Wallert</a><script data-card-contents-for-user="51706210" type="text/json">{"id":51706210,"first_name":"Arie","last_name":"Wallert","domain_name":"independent","page_name":"WallertArie","display_name":"Arie Wallert","profile_url":"https://independent.academia.edu/WallertArie?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_66347396 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="66347396"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 66347396, container: ".js-paper-rank-work_66347396", }); 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$(".js-view-count[data-work-id=66347396]").text(description); $(".js-view-count-work_66347396").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_66347396").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="66347396"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="128" rel="nofollow" href="https://www.academia.edu/Documents/in/History">History</a>, <script data-card-contents-for-ri="128" type="text/json">{"id":128,"name":"History","url":"https://www.academia.edu/Documents/in/History?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1236" rel="nofollow" href="https://www.academia.edu/Documents/in/Art">Art</a>, <script data-card-contents-for-ri="1236" type="text/json">{"id":1236,"name":"Art","url":"https://www.academia.edu/Documents/in/Art?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5081" rel="nofollow" href="https://www.academia.edu/Documents/in/Painting">Painting</a><script data-card-contents-for-ri="5081" type="text/json">{"id":5081,"name":"Painting","url":"https://www.academia.edu/Documents/in/Painting?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=66347396]'), work: {"id":66347396,"title":"Two still-life paintings by Jan van Huysum: an examination of painting technique in relation to documentary and technical evidence","created_at":"2021-12-29T03:07:13.908-08:00","url":"https://www.academia.edu/66347396/Two_still_life_paintings_by_Jan_van_Huysum_an_examination_of_painting_technique_in_relation_to_documentary_and_technical_evidence?f_ri=3091","dom_id":"work_66347396","summary":"Two still-life paintings were scientifically examined in order to gain insights about Dutch painter Jan van Huysum\u0026#39;s (1682-1749) purportedly secretive paintings techniques, comparing the actual works to information obtained in the few available historical technical sources. Surface investigations included macroscopic and microscopic examination, infrared reflectography and x-radiography, while material characterization of the ground, organic and inorganic pigments and binding media was carried out with a range of non-destructive and destructive analytical techniques. The belief that the two works were an original pair of pendant paintings was disproved due to substantial technical differences in the wood support, ground layers and use of yellow pigment. It was also found that Van Huysum\u0026#39;s use of newly developed pigments (Prussian blue, Naples yellow) had lead to changes in his painting technique, which no longer corresponded to those described in the period\u0026#39;s manuals, an...","downloadable_attachments":[],"ordered_authors":[{"id":51706210,"first_name":"Arie","last_name":"Wallert","domain_name":"independent","page_name":"WallertArie","display_name":"Arie Wallert","profile_url":"https://independent.academia.edu/WallertArie?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":128,"name":"History","url":"https://www.academia.edu/Documents/in/History?f_ri=3091","nofollow":true},{"id":1236,"name":"Art","url":"https://www.academia.edu/Documents/in/Art?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":5081,"name":"Painting","url":"https://www.academia.edu/Documents/in/Painting?f_ri=3091","nofollow":true},{"id":10655,"name":"Scanning Electron Microscopy","url":"https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy?f_ri=3091"},{"id":53132,"name":"Analysis","url":"https://www.academia.edu/Documents/in/Analysis?f_ri=3091"},{"id":121939,"name":"Technique","url":"https://www.academia.edu/Documents/in/Technique?f_ri=3091"},{"id":144839,"name":"Ground","url":"https://www.academia.edu/Documents/in/Ground?f_ri=3091"},{"id":145891,"name":"Pigment","url":"https://www.academia.edu/Documents/in/Pigment?f_ri=3091"},{"id":166448,"name":"Gas Chromatography","url":"https://www.academia.edu/Documents/in/Gas_Chromatography?f_ri=3091"},{"id":171575,"name":"Material","url":"https://www.academia.edu/Documents/in/Material?f_ri=3091"},{"id":229744,"name":"Support","url":"https://www.academia.edu/Documents/in/Support?f_ri=3091"},{"id":305691,"name":"Prussian Blue","url":"https://www.academia.edu/Documents/in/Prussian_Blue?f_ri=3091"},{"id":310981,"name":"Oil painting","url":"https://www.academia.edu/Documents/in/Oil_painting?f_ri=3091"},{"id":375252,"name":"Radiography","url":"https://www.academia.edu/Documents/in/Radiography?f_ri=3091"},{"id":386527,"name":"X ray diffraction","url":"https://www.academia.edu/Documents/in/X_ray_diffraction?f_ri=3091"},{"id":404000,"name":"Cross Section","url":"https://www.academia.edu/Documents/in/Cross_Section?f_ri=3091"},{"id":1006711,"name":"X ray Fluorescence","url":"https://www.academia.edu/Documents/in/X_ray_Fluorescence?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62462449" data-work_id="62462449" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" rel="nofollow" href="https://www.academia.edu/62462449/Thickness_estimation_of_fluorescent_sections_using_a_CSLM">Thickness estimation of fluorescent sections using a CSLM</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/62462449" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4e5210b72b691a288bed737a933cd95e" rel="nofollow" data-download="{"attachment_id":75218324,"asset_id":62462449,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen 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Brismar","profile_url":"https://independent.academia.edu/HBrismar?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_62462449 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="62462449"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 62462449, container: ".js-paper-rank-work_62462449", }); });</script></li><li class="js-percentile-work_62462449 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 62462449; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_62462449"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_62462449 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="62462449"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 62462449; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=62462449]").text(description); $(".js-view-count-work_62462449").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_62462449").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="62462449"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="56" rel="nofollow" href="https://www.academia.edu/Documents/in/Materials_Engineering">Materials Engineering</a>, <script data-card-contents-for-ri="56" type="text/json">{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="505" rel="nofollow" href="https://www.academia.edu/Documents/in/Condensed_Matter_Physics">Condensed Matter Physics</a>, <script data-card-contents-for-ri="505" type="text/json">{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7698" rel="nofollow" href="https://www.academia.edu/Documents/in/Fluorescence">Fluorescence</a><script data-card-contents-for-ri="7698" type="text/json">{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=62462449]'), work: {"id":62462449,"title":"Thickness estimation of fluorescent sections using a 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Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=3091","nofollow":true},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=3091"},{"id":151141,"name":"Refractive Index","url":"https://www.academia.edu/Documents/in/Refractive_Index?f_ri=3091"},{"id":1112728,"name":"Point Spread Function","url":"https://www.academia.edu/Documents/in/Point_Spread_Function?f_ri=3091"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091"},{"id":1773970,"name":"Section","url":"https://www.academia.edu/Documents/in/Section?f_ri=3091"},{"id":2187257,"name":"FWHM","url":"https://www.academia.edu/Documents/in/FWHM?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_51374974" data-work_id="51374974" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/51374974/Mixed_infection_involving_Actinomyces_Aggregatibacter_and_Fusobacterium_species_presenting_as_perispinal_tumor">Mixed infection involving Actinomyces, Aggregatibacter, and Fusobacterium species presenting as perispinal tumor</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A representative case in which a polymicrobial infection involving Fusobacterium nucleatum, Actinomyces israelii and Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans was initially diagnosed as malignancy in an edentulous... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_51374974" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A representative case in which a polymicrobial infection involving Fusobacterium nucleatum, Actinomyces israelii and Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans was initially diagnosed as malignancy in an edentulous patient. Additional history obtained after the nature of the syndrome was elucidated revealed that he had had his two remaining teeth extracted four months prior to this episode.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/51374974" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0f44ecb6f4d830140934da860651c7ea" rel="nofollow" data-download="{"attachment_id":69126219,"asset_id":51374974,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/69126219/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="36202145" href="https://independent.academia.edu/EllenBaron">Ellen Baron</a><script data-card-contents-for-user="36202145" type="text/json">{"id":36202145,"first_name":"Ellen","last_name":"Baron","domain_name":"independent","page_name":"EllenBaron","display_name":"Ellen Baron","profile_url":"https://independent.academia.edu/EllenBaron?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_51374974 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="51374974"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 51374974, container: ".js-paper-rank-work_51374974", }); 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$(".js-view-count[data-work-id=51374974]").text(description); $(".js-view-count-work_51374974").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_51374974").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="51374974"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" rel="nofollow" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="162159" rel="nofollow" href="https://www.academia.edu/Documents/in/Differential_Diagnosis">Differential Diagnosis</a><script data-card-contents-for-ri="162159" type="text/json">{"id":162159,"name":"Differential Diagnosis","url":"https://www.academia.edu/Documents/in/Differential_Diagnosis?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=51374974]'), work: {"id":51374974,"title":"Mixed infection involving Actinomyces, Aggregatibacter, and Fusobacterium species presenting as perispinal tumor","created_at":"2021-09-06T12:42:30.622-07:00","url":"https://www.academia.edu/51374974/Mixed_infection_involving_Actinomyces_Aggregatibacter_and_Fusobacterium_species_presenting_as_perispinal_tumor?f_ri=3091","dom_id":"work_51374974","summary":"A representative case in which a polymicrobial infection involving Fusobacterium nucleatum, Actinomyces israelii and Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans was initially diagnosed as malignancy in an edentulous patient. Additional history obtained after the nature of the syndrome was elucidated revealed that he had had his two remaining teeth extracted four months prior to this episode.","downloadable_attachments":[{"id":69126219,"asset_id":51374974,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":36202145,"first_name":"Ellen","last_name":"Baron","domain_name":"independent","page_name":"EllenBaron","display_name":"Ellen Baron","profile_url":"https://independent.academia.edu/EllenBaron?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=3091","nofollow":true},{"id":162159,"name":"Differential Diagnosis","url":"https://www.academia.edu/Documents/in/Differential_Diagnosis?f_ri=3091","nofollow":true},{"id":444298,"name":"Actinomyces","url":"https://www.academia.edu/Documents/in/Actinomyces?f_ri=3091"},{"id":482165,"name":"Myelitis","url":"https://www.academia.edu/Documents/in/Myelitis?f_ri=3091"},{"id":546401,"name":"Tooth Extraction","url":"https://www.academia.edu/Documents/in/Tooth_Extraction?f_ri=3091"},{"id":777494,"name":"Actinomycosis","url":"https://www.academia.edu/Documents/in/Actinomycosis?f_ri=3091"},{"id":3605693,"name":"Fusobacterium nucleatum","url":"https://www.academia.edu/Documents/in/Fusobacterium_nucleatum?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_48530657" data-work_id="48530657" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48530657/Secretagogin_is_a_new_neuroendocrine_marker_in_the_human_prostate">Secretagogin is a new neuroendocrine marker in the human prostate</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">BACKGROUND. Neuroendocrine (NE) differentiation in prostate cancer (PCa), promoted by NE cell secreted products, appears to be associated with tumor progression, poor prognosis, and hormone-refractory disease. We recently reported... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48530657" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">BACKGROUND. Neuroendocrine (NE) differentiation in prostate cancer (PCa), promoted by NE cell secreted products, appears to be associated with tumor progression, poor prognosis, and hormone-refractory disease. We recently reported secretagogin, a hexa-EF-hand Ca 2þ binding protein, as a novel NE marker in carcinoid tumors of the lung and the gastrointestinal tract. The present study analyzes the expression of secretagogin in normal and malign prostate tissue. METHODS. We analyzed immunoreactivity for secretagogin, chromogranin A (CgA), neuron specific enolase (NSE), and synaptophysin (SYN) in consecutive sections from 87 formalin-fixed paraffin-embedded (FFPE) benign hyperplastic (n ¼ 10) and prostate adenocarcinoma (n ¼ 77) specimens. The intracellular distribution of secretagogin, CgA, and NSE was examined by confocal fluorescent microscopy, and we characterized secretagogin in eight samples by Western blotting. RESULTS. Secretagogin is cytoplasmic and nuclear expressed in NE and NE differentiated cells, and to a lesser extent in epithelial cells, in the benign prostate and prostate adenocarcinoma cells. Secretagogin stained 82% (46/56) of benign and 71% (48/68) of prostate adenocarcinomas and co-localized with the NE markers CgA and NSE. The expression of secretagogin is significantly correlated to CgA (P < 0.001) and NSE (P < 0.048) in prostate adenocarcinoma and to CgA in normal epithelium (P < 0.028).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48530657" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="703810a0e8c39def09ed6b8432fbce52" rel="nofollow" data-download="{"attachment_id":67134223,"asset_id":48530657,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/67134223/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="44840497" href="https://independent.academia.edu/LudwigWagner2">Ludwig Wagner</a><script data-card-contents-for-user="44840497" type="text/json">{"id":44840497,"first_name":"Ludwig","last_name":"Wagner","domain_name":"independent","page_name":"LudwigWagner2","display_name":"Ludwig Wagner","profile_url":"https://independent.academia.edu/LudwigWagner2?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_48530657 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48530657"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48530657, container: ".js-paper-rank-work_48530657", }); });</script></li><li class="js-percentile-work_48530657 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 48530657; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_48530657"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_48530657 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="48530657"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 48530657; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=48530657]").text(description); $(".js-view-count-work_48530657").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_48530657").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="48530657"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="892" rel="nofollow" href="https://www.academia.edu/Documents/in/Statistics">Statistics</a>, <script data-card-contents-for-ri="892" type="text/json">{"id":892,"name":"Statistics","url":"https://www.academia.edu/Documents/in/Statistics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3243" rel="nofollow" href="https://www.academia.edu/Documents/in/Nonparametric_Statistics">Nonparametric Statistics</a>, <script data-card-contents-for-ri="3243" type="text/json">{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7698" rel="nofollow" href="https://www.academia.edu/Documents/in/Fluorescence">Fluorescence</a><script data-card-contents-for-ri="7698" type="text/json">{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48530657]'), work: {"id":48530657,"title":"Secretagogin is a new neuroendocrine marker in the human prostate","created_at":"2021-05-04T22:24:59.338-07:00","url":"https://www.academia.edu/48530657/Secretagogin_is_a_new_neuroendocrine_marker_in_the_human_prostate?f_ri=3091","dom_id":"work_48530657","summary":"BACKGROUND. Neuroendocrine (NE) differentiation in prostate cancer (PCa), promoted by NE cell secreted products, appears to be associated with tumor progression, poor prognosis, and hormone-refractory disease. We recently reported secretagogin, a hexa-EF-hand Ca 2þ binding protein, as a novel NE marker in carcinoid tumors of the lung and the gastrointestinal tract. The present study analyzes the expression of secretagogin in normal and malign prostate tissue. METHODS. We analyzed immunoreactivity for secretagogin, chromogranin A (CgA), neuron specific enolase (NSE), and synaptophysin (SYN) in consecutive sections from 87 formalin-fixed paraffin-embedded (FFPE) benign hyperplastic (n ¼ 10) and prostate adenocarcinoma (n ¼ 77) specimens. The intracellular distribution of secretagogin, CgA, and NSE was examined by confocal fluorescent microscopy, and we characterized secretagogin in eight samples by Western blotting. RESULTS. Secretagogin is cytoplasmic and nuclear expressed in NE and NE differentiated cells, and to a lesser extent in epithelial cells, in the benign prostate and prostate adenocarcinoma cells. Secretagogin stained 82% (46/56) of benign and 71% (48/68) of prostate adenocarcinomas and co-localized with the NE markers CgA and NSE. The expression of secretagogin is significantly correlated to CgA (P \u003c 0.001) and NSE (P \u003c 0.048) in prostate adenocarcinoma and to CgA in normal epithelium (P \u003c 0.028).","downloadable_attachments":[{"id":67134223,"asset_id":48530657,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":44840497,"first_name":"Ludwig","last_name":"Wagner","domain_name":"independent","page_name":"LudwigWagner2","display_name":"Ludwig Wagner","profile_url":"https://independent.academia.edu/LudwigWagner2?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":892,"name":"Statistics","url":"https://www.academia.edu/Documents/in/Statistics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=3091","nofollow":true},{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=3091","nofollow":true},{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy?f_ri=3091"},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=3091"},{"id":161176,"name":"The","url":"https://www.academia.edu/Documents/in/The?f_ri=3091"},{"id":161553,"name":"Prostate","url":"https://www.academia.edu/Documents/in/Prostate?f_ri=3091"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=3091"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=3091"},{"id":889628,"name":"Calcium Binding Proteins","url":"https://www.academia.edu/Documents/in/Calcium_Binding_Proteins?f_ri=3091"},{"id":1120983,"name":"Synaptophysin","url":"https://www.academia.edu/Documents/in/Synaptophysin?f_ri=3091"},{"id":1745478,"name":"Adenocarcinoma","url":"https://www.academia.edu/Documents/in/Adenocarcinoma?f_ri=3091"},{"id":2439467,"name":"Chromogranin A","url":"https://www.academia.edu/Documents/in/Chromogranin_A?f_ri=3091"},{"id":3087971,"name":"Blotting","url":"https://www.academia.edu/Documents/in/Blotting?f_ri=3091"},{"id":3205111,"name":"Prostatic neoplasms","url":"https://www.academia.edu/Documents/in/Prostatic_neoplasms?f_ri=3091"},{"id":3789883,"name":"Paediatrics and reproductive medicine","url":"https://www.academia.edu/Documents/in/Paediatrics_and_reproductive_medicine?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_39900904" data-work_id="39900904" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/39900904/GFP_based_FRET_analysis_in_live_cells">GFP-based FRET analysis in live cells</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Fluorescence resonance energy transfer (FRET) is a widely utilized optical technique for measuring small distances of 1-10 nm in live cells. In recent years, its application has been greatly popularized by the discovery of green... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_39900904" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Fluorescence resonance energy transfer (FRET) is a widely utilized optical technique for measuring small distances of 1-10 nm in live cells. In recent years, its application has been greatly popularized by the discovery of green fluorescent protein (GFP) and many improved variants which make good donor-acceptor fluorophore pairs. GFP-based proteins are structurally stable, relatively inert, and can be reliably attached to points of interest. The combination of easy access to the GFP-based FRET technique and its obvious usefulness in many applications can lead to complacency. Potential problems such as light contaminants, e.g., bleed-through and cross-talk, and inconsistent donor and acceptor concentrations are easily overlooked and can lead to errors in FRET calculation and data interpretation. In this article, we outline possible pitfalls of GFP-based FRET and approaches that address these issues, including a "Spectra FRET" technique that can be easily applied to live cell studies.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/39900904" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2d027e38d7adcab8e5f79a7bf96b0e62" rel="nofollow" data-download="{"attachment_id":60086918,"asset_id":39900904,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/60086918/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="108365127" href="https://gdufs.academia.edu/JieZheng">Jie Zheng</a><script data-card-contents-for-user="108365127" type="text/json">{"id":108365127,"first_name":"Jie","last_name":"Zheng","domain_name":"gdufs","page_name":"JieZheng","display_name":"Jie Zheng","profile_url":"https://gdufs.academia.edu/JieZheng?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_39900904 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="39900904"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 39900904, container: ".js-paper-rank-work_39900904", }); 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In recent years, its application has been greatly popularized by the discovery of green fluorescent protein (GFP) and many improved variants which make good donor-acceptor fluorophore pairs. GFP-based proteins are structurally stable, relatively inert, and can be reliably attached to points of interest. The combination of easy access to the GFP-based FRET technique and its obvious usefulness in many applications can lead to complacency. Potential problems such as light contaminants, e.g., bleed-through and cross-talk, and inconsistent donor and acceptor concentrations are easily overlooked and can lead to errors in FRET calculation and data interpretation. In this article, we outline possible pitfalls of GFP-based FRET and approaches that address these issues, including a \"Spectra FRET\" technique that can be easily applied to live cell studies.","downloadable_attachments":[{"id":60086918,"asset_id":39900904,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":108365127,"first_name":"Jie","last_name":"Zheng","domain_name":"gdufs","page_name":"JieZheng","display_name":"Jie Zheng","profile_url":"https://gdufs.academia.edu/JieZheng?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":237,"name":"Cognitive Science","url":"https://www.academia.edu/Documents/in/Cognitive_Science?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":5427,"name":"Spectroscopy","url":"https://www.academia.edu/Documents/in/Spectroscopy?f_ri=3091","nofollow":true},{"id":37384,"name":"Fluorescence Resonance Energy Transfer","url":"https://www.academia.edu/Documents/in/Fluorescence_Resonance_Energy_Transfer?f_ri=3091","nofollow":true},{"id":50487,"name":"Protein-Protein Interaction","url":"https://www.academia.edu/Documents/in/Protein-Protein_Interaction?f_ri=3091"},{"id":61474,"name":"Brain","url":"https://www.academia.edu/Documents/in/Brain?f_ri=3091"},{"id":67104,"name":"Data Interpretation","url":"https://www.academia.edu/Documents/in/Data_Interpretation?f_ri=3091"},{"id":121939,"name":"Technique","url":"https://www.academia.edu/Documents/in/Technique?f_ri=3091"},{"id":202410,"name":"Green Fluorescent Protein","url":"https://www.academia.edu/Documents/in/Green_Fluorescent_Protein?f_ri=3091"},{"id":325749,"name":"Cells","url":"https://www.academia.edu/Documents/in/Cells?f_ri=3091"},{"id":416713,"name":"Spectrometry","url":"https://www.academia.edu/Documents/in/Spectrometry?f_ri=3091"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_5967730" data-work_id="5967730" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/5967730/Variable_angle_total_internal_reflection_fluorescence_microscopy_VA_TIRFM_realization_and_application_of_a_compact_illumination_device">Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM): realization and application of a compact illumination device</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A novel compact illumination device in variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) is described. This device replaces the standard condensor of an upright microscope. Light from different laser sources is... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_5967730" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A novel compact illumination device in variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) is described. This device replaces the standard condensor of an upright microscope. Light from different laser sources is delivered via a monomode fibre and focused onto identical parts of a sample under variable angles of total internal reflection. Thus, fluorophores in close proximity to a cell-substrate interface are excited by an evanescent wave with variable penetration depth, and localized with high (nanometre) axial resolution. In addition to quantitative measurements in solution, fluorescence markers of the cytoplasm and the plasma membrane, i.e. calcein and laurdan, were examined using cultivated endothelial cells. Distances between the glass substrate and the plasma membrane were determined using the mathematical algorithm of a four-layer model, as well as a Gaussian-shaped intensity profile of the illumination spot on the samples. Distances between 0 and 30 nm in focal contacts and between 100 and 300 nm in other parts of the cell were thus determined. In addition to measurements of cell-substrate topology, the illumination device appears appropriate for numerous applications in which high axial resolution is required, e.g. experiments on endocytosis or exocytosis, as well as measurements of ion concentrations proximal to the plasma membrane. The compact illumination device is also suitable for combining TIRFM with further innovative techniques, e.g. time-resolved fluorescence spectroscopy, fluorescence lifetime imaging (FLIM) or fluorescence resonance energy transfer (FRET).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/5967730" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b07ca24141383ed6fae9d35a701a1fb8" rel="nofollow" data-download="{"attachment_id":49072910,"asset_id":5967730,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49072910/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="8897297" href="https://uni-ulm.academia.edu/RudolfSteiner">Rudolf Steiner</a><script data-card-contents-for-user="8897297" type="text/json">{"id":8897297,"first_name":"Rudolf","last_name":"Steiner","domain_name":"uni-ulm","page_name":"RudolfSteiner","display_name":"Rudolf Steiner","profile_url":"https://uni-ulm.academia.edu/RudolfSteiner?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_5967730 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="5967730"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 5967730, container: ".js-paper-rank-work_5967730", }); 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This device replaces the standard condensor of an upright microscope. Light from different laser sources is delivered via a monomode fibre and focused onto identical parts of a sample under variable angles of total internal reflection. Thus, fluorophores in close proximity to a cell-substrate interface are excited by an evanescent wave with variable penetration depth, and localized with high (nanometre) axial resolution. In addition to quantitative measurements in solution, fluorescence markers of the cytoplasm and the plasma membrane, i.e. calcein and laurdan, were examined using cultivated endothelial cells. Distances between the glass substrate and the plasma membrane were determined using the mathematical algorithm of a four-layer model, as well as a Gaussian-shaped intensity profile of the illumination spot on the samples. Distances between 0 and 30 nm in focal contacts and between 100 and 300 nm in other parts of the cell were thus determined. In addition to measurements of cell-substrate topology, the illumination device appears appropriate for numerous applications in which high axial resolution is required, e.g. experiments on endocytosis or exocytosis, as well as measurements of ion concentrations proximal to the plasma membrane. The compact illumination device is also suitable for combining TIRFM with further innovative techniques, e.g. time-resolved fluorescence spectroscopy, fluorescence lifetime imaging (FLIM) or fluorescence resonance energy transfer (FRET).","downloadable_attachments":[{"id":49072910,"asset_id":5967730,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":8897297,"first_name":"Rudolf","last_name":"Steiner","domain_name":"uni-ulm","page_name":"RudolfSteiner","display_name":"Rudolf Steiner","profile_url":"https://uni-ulm.academia.edu/RudolfSteiner?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":300,"name":"Mathematics","url":"https://www.academia.edu/Documents/in/Mathematics?f_ri=3091","nofollow":true},{"id":505,"name":"Condensed Matter 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It has been derived from an in situ bright field microscope that is able to measure cell density in bioreactors during fermentation... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_8283529" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A new-type in situ probe has been developed to acquire dark field images of yeast in bioreactors. It has been derived from an in situ bright field microscope that is able to measure cell density in bioreactors during fermentation processes. The illumination part of the probe has been replaced with a dark field device, in which an aspheric condenser is used, so that high contrast dark field images can be obtained. The technique of second imaging is implemented to improve the sharpness of the images by means of a relay lens. 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href="https://www.academia.edu/27624470/Early_Healing_of_Transcolonic_and_Transgastric_Natural_Orifice_Transluminal_Endoscopic_Surgery_Access_Sites">Early Healing of Transcolonic and Transgastric Natural Orifice Transluminal Endoscopic Surgery Access Sites</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27624470" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="88e98692f3a91e2e56f16ac131380609" rel="nofollow" data-download="{"attachment_id":47888515,"asset_id":27624470,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47888515/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="51823893" href="https://independent.academia.edu/GiorgioPietramaggiori">Giorgio Pietramaggiori</a><script data-card-contents-for-user="51823893" 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() { var workId = 27624470; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_27624470"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_27624470 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="27624470"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27624470; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27624470]").text(description); $(".js-view-count-work_27624470").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_27624470").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="27624470"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="8991" rel="nofollow" href="https://www.academia.edu/Documents/in/Wound_Healing">Wound Healing</a>, <script data-card-contents-for-ri="8991" type="text/json">{"id":8991,"name":"Wound Healing","url":"https://www.academia.edu/Documents/in/Wound_Healing?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9334" rel="nofollow" href="https://www.academia.edu/Documents/in/Inflammation">Inflammation</a>, <script data-card-contents-for-ri="9334" type="text/json">{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="227324" rel="nofollow" href="https://www.academia.edu/Documents/in/Colonoscopy">Colonoscopy</a><script data-card-contents-for-ri="227324" type="text/json">{"id":227324,"name":"Colonoscopy","url":"https://www.academia.edu/Documents/in/Colonoscopy?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=27624470]'), work: {"id":27624470,"title":"Early Healing of Transcolonic and Transgastric Natural Orifice Transluminal Endoscopic Surgery Access Sites","created_at":"2016-08-08T07:49:26.141-07:00","url":"https://www.academia.edu/27624470/Early_Healing_of_Transcolonic_and_Transgastric_Natural_Orifice_Transluminal_Endoscopic_Surgery_Access_Sites?f_ri=3091","dom_id":"work_27624470","summary":null,"downloadable_attachments":[{"id":47888515,"asset_id":27624470,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":51823893,"first_name":"Giorgio","last_name":"Pietramaggiori","domain_name":"independent","page_name":"GiorgioPietramaggiori","display_name":"Giorgio Pietramaggiori","profile_url":"https://independent.academia.edu/GiorgioPietramaggiori?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":8991,"name":"Wound Healing","url":"https://www.academia.edu/Documents/in/Wound_Healing?f_ri=3091","nofollow":true},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=3091","nofollow":true},{"id":227324,"name":"Colonoscopy","url":"https://www.academia.edu/Documents/in/Colonoscopy?f_ri=3091","nofollow":true},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=3091"},{"id":343756,"name":"Gastrointestinal Endoscopy","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Endoscopy?f_ri=3091"},{"id":372622,"name":"Granulation Tissue","url":"https://www.academia.edu/Documents/in/Granulation_Tissue?f_ri=3091"},{"id":993152,"name":"Sus Scrofa","url":"https://www.academia.edu/Documents/in/Sus_Scrofa?f_ri=3091"},{"id":1229542,"name":"Laparoscopy","url":"https://www.academia.edu/Documents/in/Laparoscopy?f_ri=3091"},{"id":1295203,"name":"Gastroscopy","url":"https://www.academia.edu/Documents/in/Gastroscopy?f_ri=3091"},{"id":1863718,"name":"The American","url":"https://www.academia.edu/Documents/in/The_American?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_11344660" data-work_id="11344660" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/11344660/Joseph_Listers_domestic_science">Joseph Lister's domestic science</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/11344660" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2200301aaa3e1f6b23fbbaf206f6f1e6" rel="nofollow" data-download="{"attachment_id":46753981,"asset_id":11344660,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46753981/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="27531616" href="https://independent.academia.edu/RuthRichardson">Ruth Richardson</a><script data-card-contents-for-user="27531616" type="text/json">{"id":27531616,"first_name":"Ruth","last_name":"Richardson","domain_name":"independent","page_name":"RuthRichardson","display_name":"Ruth Richardson","profile_url":"https://independent.academia.edu/RuthRichardson?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_11344660 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="11344660"><i class="u-m1x fa 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view-count u-mr2x" data-work-id="11344660"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 11344660; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=11344660]").text(description); $(".js-view-count-work_11344660").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_11344660").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="11344660"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">4</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3284" rel="nofollow" href="https://www.academia.edu/Documents/in/Bacteriology">Bacteriology</a>, <script data-card-contents-for-ri="3284" type="text/json">{"id":3284,"name":"Bacteriology","url":"https://www.academia.edu/Documents/in/Bacteriology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="30494" rel="nofollow" href="https://www.academia.edu/Documents/in/England">England</a>, <script data-card-contents-for-ri="30494" type="text/json">{"id":30494,"name":"England","url":"https://www.academia.edu/Documents/in/England?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="243629" rel="nofollow" href="https://www.academia.edu/Documents/in/Lancet">Lancet</a><script data-card-contents-for-ri="243629" type="text/json">{"id":243629,"name":"Lancet","url":"https://www.academia.edu/Documents/in/Lancet?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=11344660]'), work: {"id":11344660,"title":"Joseph Lister's domestic science","created_at":"2015-03-09T17:04:50.240-07:00","url":"https://www.academia.edu/11344660/Joseph_Listers_domestic_science?f_ri=3091","dom_id":"work_11344660","summary":null,"downloadable_attachments":[{"id":46753981,"asset_id":11344660,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":27531616,"first_name":"Ruth","last_name":"Richardson","domain_name":"independent","page_name":"RuthRichardson","display_name":"Ruth Richardson","profile_url":"https://independent.academia.edu/RuthRichardson?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":3284,"name":"Bacteriology","url":"https://www.academia.edu/Documents/in/Bacteriology?f_ri=3091","nofollow":true},{"id":30494,"name":"England","url":"https://www.academia.edu/Documents/in/England?f_ri=3091","nofollow":true},{"id":243629,"name":"Lancet","url":"https://www.academia.edu/Documents/in/Lancet?f_ri=3091","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24103624" data-work_id="24103624" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24103624/Traditional_microscopy_instruction_versus_process_oriented_virtual_microscopy_instruction_a_naturalistic_experiment_with_control_group">Traditional microscopy instruction versus process-oriented virtual microscopy instruction: a naturalistic experiment with control group</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Virtual microscopy is being introduced in medical education as an approach for learning how to interpret information in microscopic specimens. It is, however, far from evident how to incorporate its use into existing teaching practice.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24103624" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Virtual microscopy is being introduced in medical education as an approach for learning how to interpret information in microscopic specimens. It is, however, far from evident how to incorporate its use into existing teaching practice. The aim of the study was to explore the consequences of introducing virtual microscopy tasks into an undergraduate pathology course in an attempt to render the instruction more process-oriented. The research questions were: 1) How is virtual microscopy perceived by students? 2) Does work on virtual microscopy tasks contribute to improvement in performance in microscopic pathology in comparison with attending assistant-led demonstrations only? During a one-week period, an experimental group completed three sets of virtual microscopy homework assignments in addition to attending demonstrations. A control group attended the demonstrations only. Performance in microscopic pathology was measured by a pre-test and a post-test. Student perceptions of regular...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24103624" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3d554d00018ef929fa87468ac49bdb00" rel="nofollow" data-download="{"attachment_id":44467065,"asset_id":24103624,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44467065/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="687519" href="https://gu-se.academia.edu/RogerS%C3%A4lj%C3%B6">Roger Säljö</a><script data-card-contents-for-user="687519" type="text/json">{"id":687519,"first_name":"Roger","last_name":"Säljö","domain_name":"gu-se","page_name":"RogerSäljö","display_name":"Roger Säljö","profile_url":"https://gu-se.academia.edu/RogerS%C3%A4lj%C3%B6?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24103624 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24103624"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24103624, container: ".js-paper-rank-work_24103624", }); });</script></li><li class="js-percentile-work_24103624 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24103624; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_24103624"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_24103624 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="24103624"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24103624; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24103624]").text(description); $(".js-view-count-work_24103624").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24103624").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24103624"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="165" rel="nofollow" href="https://www.academia.edu/Documents/in/Pathology">Pathology</a>, <script data-card-contents-for-ri="165" type="text/json">{"id":165,"name":"Pathology","url":"https://www.academia.edu/Documents/in/Pathology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37859" rel="nofollow" href="https://www.academia.edu/Documents/in/Diagnostic_Pathology">Diagnostic Pathology</a>, <script data-card-contents-for-ri="37859" type="text/json">{"id":37859,"name":"Diagnostic Pathology","url":"https://www.academia.edu/Documents/in/Diagnostic_Pathology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="77936" rel="nofollow" href="https://www.academia.edu/Documents/in/Text">Text</a><script data-card-contents-for-ri="77936" type="text/json">{"id":77936,"name":"Text","url":"https://www.academia.edu/Documents/in/Text?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24103624]'), work: {"id":24103624,"title":"Traditional microscopy instruction versus process-oriented virtual microscopy instruction: a naturalistic experiment with control group","created_at":"2016-04-06T01:24:31.770-07:00","url":"https://www.academia.edu/24103624/Traditional_microscopy_instruction_versus_process_oriented_virtual_microscopy_instruction_a_naturalistic_experiment_with_control_group?f_ri=3091","dom_id":"work_24103624","summary":"Virtual microscopy is being introduced in medical education as an approach for learning how to interpret information in microscopic specimens. It is, however, far from evident how to incorporate its use into existing teaching practice. The aim of the study was to explore the consequences of introducing virtual microscopy tasks into an undergraduate pathology course in an attempt to render the instruction more process-oriented. The research questions were: 1) How is virtual microscopy perceived by students? 2) Does work on virtual microscopy tasks contribute to improvement in performance in microscopic pathology in comparison with attending assistant-led demonstrations only? During a one-week period, an experimental group completed three sets of virtual microscopy homework assignments in addition to attending demonstrations. A control group attended the demonstrations only. Performance in microscopic pathology was measured by a pre-test and a post-test. Student perceptions of regular...","downloadable_attachments":[{"id":44467065,"asset_id":24103624,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":687519,"first_name":"Roger","last_name":"Säljö","domain_name":"gu-se","page_name":"RogerSäljö","display_name":"Roger Säljö","profile_url":"https://gu-se.academia.edu/RogerS%C3%A4lj%C3%B6?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":165,"name":"Pathology","url":"https://www.academia.edu/Documents/in/Pathology?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":37859,"name":"Diagnostic Pathology","url":"https://www.academia.edu/Documents/in/Diagnostic_Pathology?f_ri=3091","nofollow":true},{"id":77936,"name":"Text","url":"https://www.academia.edu/Documents/in/Text?f_ri=3091","nofollow":true},{"id":93975,"name":"Computer Assisted Instruction","url":"https://www.academia.edu/Documents/in/Computer_Assisted_Instruction?f_ri=3091"},{"id":99364,"name":"Undergraduate medical education","url":"https://www.academia.edu/Documents/in/Undergraduate_medical_education?f_ri=3091"},{"id":255094,"name":"Computer User Interface Design","url":"https://www.academia.edu/Documents/in/Computer_User_Interface_Design?f_ri=3091"},{"id":396054,"name":"Diagnostic","url":"https://www.academia.edu/Documents/in/Diagnostic?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16392926 coauthored" data-work_id="16392926" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16392926/Combined_3DISCO_clearing_method_retrograde_tracer_and_ultramicroscopy_to_map_corneal_neurons_in_a_whole_adult_mouse_trigeminal_ganglion">Combined 3DISCO clearing method, retrograde tracer and ultramicroscopy to map corneal neurons in a whole adult mouse trigeminal ganglion</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Tissue clearing and subsequent imaging of intact transparent tissues have provided an innovative way to analyze anatomical pathways in the nervous system. In this study, we combined a recent 3-dimensional imaging of solvent cleared organ... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16392926" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Tissue clearing and subsequent imaging of intact transparent tissues have provided an innovative way to analyze anatomical pathways in the nervous system. In this study, we combined a recent 3-dimensional imaging of solvent cleared organ (3DISCO) procedure, light-sheet microscopy, fluorescent retrograde tracer, and Imaris software to 3D map corneal sensory neurons within a whole adult mouse trigeminal ganglion (TG). We first established the optimized steps to easily and rapidly clear a fixed TG. We found that the 3DISCO procedure gave excellent results and took less than 3 h to clear the TG. In a second set of experiments, a retrograde tracer (cholera toxin B Alexa 594-conjugated) was applied to de-epithelialized cornea to retrograde-labeled corneal sensory neurons. Two days later, TGs were cleared by the 3DISCO method and serial imaging was performed using light-sheet ultramicroscopic technology. High-resolution images of labeled neurons can be easily and rapidly obtained from a 3D...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16392926" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="325bbeb2eaab7b55c8f3ff2962775aa2" rel="nofollow" data-download="{"attachment_id":42502736,"asset_id":16392926,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42502736/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35529701" href="https://independent.academia.edu/HanenKhabou">Hanen Khabou</a><script data-card-contents-for-user="35529701" type="text/json">{"id":35529701,"first_name":"Hanen","last_name":"Khabou","domain_name":"independent","page_name":"HanenKhabou","display_name":"Hanen Khabou","profile_url":"https://independent.academia.edu/HanenKhabou?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-16392926">+1</span><div class="hidden js-additional-users-16392926"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://cearc.academia.edu/ChristopheBaudouin">Christophe Baudouin</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-16392926'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-16392926').html(); 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container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16392926 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16392926"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16392926; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16392926]").text(description); $(".js-view-count-work_16392926").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16392926").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16392926"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="17721" rel="nofollow" href="https://www.academia.edu/Documents/in/Cornea">Cornea</a>, <script data-card-contents-for-ri="17721" type="text/json">{"id":17721,"name":"Cornea","url":"https://www.academia.edu/Documents/in/Cornea?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="82660" rel="nofollow" href="https://www.academia.edu/Documents/in/Sensation">Sensation</a>, <script data-card-contents-for-ri="82660" type="text/json">{"id":82660,"name":"Sensation","url":"https://www.academia.edu/Documents/in/Sensation?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" rel="nofollow" href="https://www.academia.edu/Documents/in/Mice">Mice</a><script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16392926]'), work: {"id":16392926,"title":"Combined 3DISCO clearing method, retrograde tracer and ultramicroscopy to map corneal neurons in a whole adult mouse trigeminal ganglion","created_at":"2015-10-02T01:53:09.535-07:00","url":"https://www.academia.edu/16392926/Combined_3DISCO_clearing_method_retrograde_tracer_and_ultramicroscopy_to_map_corneal_neurons_in_a_whole_adult_mouse_trigeminal_ganglion?f_ri=3091","dom_id":"work_16392926","summary":"Tissue clearing and subsequent imaging of intact transparent tissues have provided an innovative way to analyze anatomical pathways in the nervous system. In this study, we combined a recent 3-dimensional imaging of solvent cleared organ (3DISCO) procedure, light-sheet microscopy, fluorescent retrograde tracer, and Imaris software to 3D map corneal sensory neurons within a whole adult mouse trigeminal ganglion (TG). We first established the optimized steps to easily and rapidly clear a fixed TG. We found that the 3DISCO procedure gave excellent results and took less than 3 h to clear the TG. In a second set of experiments, a retrograde tracer (cholera toxin B Alexa 594-conjugated) was applied to de-epithelialized cornea to retrograde-labeled corneal sensory neurons. Two days later, TGs were cleared by the 3DISCO method and serial imaging was performed using light-sheet ultramicroscopic technology. High-resolution images of labeled neurons can be easily and rapidly obtained from a 3D...","downloadable_attachments":[{"id":42502736,"asset_id":16392926,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35529701,"first_name":"Hanen","last_name":"Khabou","domain_name":"independent","page_name":"HanenKhabou","display_name":"Hanen Khabou","profile_url":"https://independent.academia.edu/HanenKhabou?f_ri=3091","photo":"/images/s65_no_pic.png"},{"id":35674458,"first_name":"Christophe","last_name":"Baudouin","domain_name":"cearc","page_name":"ChristopheBaudouin","display_name":"Christophe Baudouin","profile_url":"https://cearc.academia.edu/ChristopheBaudouin?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":17721,"name":"Cornea","url":"https://www.academia.edu/Documents/in/Cornea?f_ri=3091","nofollow":true},{"id":82660,"name":"Sensation","url":"https://www.academia.edu/Documents/in/Sensation?f_ri=3091","nofollow":true},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=3091","nofollow":true},{"id":123287,"name":"Three Dimensional Imaging","url":"https://www.academia.edu/Documents/in/Three_Dimensional_Imaging?f_ri=3091"},{"id":359001,"name":"Optometry and Ophthalmology","url":"https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology?f_ri=3091"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_80716921" data-work_id="80716921" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/80716921/Divergent_Nod_Containing_Bradyrhizobium_sp_DOA9_with_a_Megaplasmid_and_its_Host_Range">Divergent Nod-Containing Bradyrhizobium sp. DOA9 with a Megaplasmid and its Host Range</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Bradyrhizobium sp. DOA9, a non-photosynthetic bacterial strain originally isolated from the root nodules of the legume Aeschynomene americana, is a divergent nod-containing strain. It exhibits a broad host range, being able to colonize... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_80716921" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Bradyrhizobium sp. DOA9, a non-photosynthetic bacterial strain originally isolated from the root nodules of the legume Aeschynomene americana, is a divergent nod-containing strain. It exhibits a broad host range, being able to colonize and efficiently nodulate the roots of most plants from the Dalbergioid, Millettioid, and Robinioid tribes (7 species of Papilionoideae). In all cases, nodulation was determinate. The morphology and size of DOA9 bacteroids isolated from the nodules of various species of Papilionoideae were indistinguishable from the free-living form. However, they were spherical in Arachis hypogaea nodules. GusA-tagged DOA9 also colonized rice roots as endophytes. Since broad-host-range legume symbionts often carry multiple replicons in their genome, we analyzed the replicons for symbiosis genes by electrophoresis. DOA9 carried two replicons, a chromosome (cDOA9) and single megaplasmid (pDOA9) larger than 352 kb. The genes for nodulation (nodA, B, C) and nitrogen fixation (nifH) were localized on the megaplasmid. Southern blot hybridization revealed two copies of nodA on the megaplasmid, single copies of nodB and C on the megaplasmid, and one copy each of nifH on the chromosome and megaplasmid. These results suggested that Bradyrhizobium sp. DOA9 may have the unusual combination of a broad host range, bacteroid differentiation, and symbiosis-mediating replicons.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/80716921" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9f3c73bbc7ffde8bb797b0bc659f4c1a" rel="nofollow" data-download="{"attachment_id":87006990,"asset_id":80716921,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/87006990/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="118296972" href="https://independent.academia.edu/PongpanSongwattana">Pongpan Songwattana</a><script data-card-contents-for-user="118296972" type="text/json">{"id":118296972,"first_name":"Pongpan","last_name":"Songwattana","domain_name":"independent","page_name":"PongpanSongwattana","display_name":"Pongpan Songwattana","profile_url":"https://independent.academia.edu/PongpanSongwattana?f_ri=3091","photo":"https://0.academia-photos.com/118296972/30415144/28188288/s65_pongpan.songwattana.jpg"}</script></span></span></li><li class="js-paper-rank-work_80716921 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="80716921"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 80716921, container: ".js-paper-rank-work_80716921", }); 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$(".js-view-count[data-work-id=80716921]").text(description); $(".js-view-count-work_80716921").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_80716921").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="80716921"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" rel="nofollow" href="https://www.academia.edu/Documents/in/Biology">Biology</a>, <script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" rel="nofollow" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="63551" rel="nofollow" href="https://www.academia.edu/Documents/in/Nitrogen_Fixation">Nitrogen Fixation</a><script data-card-contents-for-ri="63551" type="text/json">{"id":63551,"name":"Nitrogen Fixation","url":"https://www.academia.edu/Documents/in/Nitrogen_Fixation?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=80716921]'), work: {"id":80716921,"title":"Divergent Nod-Containing Bradyrhizobium sp. DOA9 with a Megaplasmid and its Host Range","created_at":"2022-06-04T22:51:00.122-07:00","url":"https://www.academia.edu/80716921/Divergent_Nod_Containing_Bradyrhizobium_sp_DOA9_with_a_Megaplasmid_and_its_Host_Range?f_ri=3091","dom_id":"work_80716921","summary":"Bradyrhizobium sp. DOA9, a non-photosynthetic bacterial strain originally isolated from the root nodules of the legume Aeschynomene americana, is a divergent nod-containing strain. It exhibits a broad host range, being able to colonize and efficiently nodulate the roots of most plants from the Dalbergioid, Millettioid, and Robinioid tribes (7 species of Papilionoideae). In all cases, nodulation was determinate. The morphology and size of DOA9 bacteroids isolated from the nodules of various species of Papilionoideae were indistinguishable from the free-living form. However, they were spherical in Arachis hypogaea nodules. GusA-tagged DOA9 also colonized rice roots as endophytes. Since broad-host-range legume symbionts often carry multiple replicons in their genome, we analyzed the replicons for symbiosis genes by electrophoresis. DOA9 carried two replicons, a chromosome (cDOA9) and single megaplasmid (pDOA9) larger than 352 kb. The genes for nodulation (nodA, B, C) and nitrogen fixation (nifH) were localized on the megaplasmid. Southern blot hybridization revealed two copies of nodA on the megaplasmid, single copies of nodB and C on the megaplasmid, and one copy each of nifH on the chromosome and megaplasmid. These results suggested that Bradyrhizobium sp. DOA9 may have the unusual combination of a broad host range, bacteroid differentiation, and symbiosis-mediating replicons.","downloadable_attachments":[{"id":87006990,"asset_id":80716921,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":118296972,"first_name":"Pongpan","last_name":"Songwattana","domain_name":"independent","page_name":"PongpanSongwattana","display_name":"Pongpan Songwattana","profile_url":"https://independent.academia.edu/PongpanSongwattana?f_ri=3091","photo":"https://0.academia-photos.com/118296972/30415144/28188288/s65_pongpan.songwattana.jpg"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=3091","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=3091","nofollow":true},{"id":63551,"name":"Nitrogen Fixation","url":"https://www.academia.edu/Documents/in/Nitrogen_Fixation?f_ri=3091","nofollow":true},{"id":190363,"name":"Plasmids","url":"https://www.academia.edu/Documents/in/Plasmids?f_ri=3091"},{"id":361141,"name":"Angiosperms","url":"https://www.academia.edu/Documents/in/Angiosperms?f_ri=3091"},{"id":529001,"name":"Bradyrhizobium","url":"https://www.academia.edu/Documents/in/Bradyrhizobium?f_ri=3091"},{"id":589755,"name":"Host Specificity","url":"https://www.academia.edu/Documents/in/Host_Specificity?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62462448" data-work_id="62462448" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" rel="nofollow" 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type="text/json">{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="17491" rel="nofollow" href="https://www.academia.edu/Documents/in/Macrophages">Macrophages</a><script data-card-contents-for-ri="17491" type="text/json">{"id":17491,"name":"Macrophages","url":"https://www.academia.edu/Documents/in/Macrophages?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=62462448]'), work: {"id":62462448,"title":"Confocal pH imaging of microscopic specimens using fluorescence lifetimes and phase fluorometry: influence of parameter choice on system performance","created_at":"2021-11-26T05:42:36.492-08:00","url":"https://www.academia.edu/62462448/Confocal_pH_imaging_of_microscopic_specimens_using_fluorescence_lifetimes_and_phase_fluorometry_influence_of_parameter_choice_on_system_performance?f_ri=3091","dom_id":"work_62462448","summary":null,"downloadable_attachments":[{"id":75218517,"asset_id":62462448,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50711711,"first_name":"Hjalmar","last_name":"Brismar","domain_name":"independent","page_name":"HBrismar","display_name":"Hjalmar Brismar","profile_url":"https://independent.academia.edu/HBrismar?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":17491,"name":"Macrophages","url":"https://www.academia.edu/Documents/in/Macrophages?f_ri=3091","nofollow":true},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=3091"},{"id":55547,"name":"Fluorescence Lifetime","url":"https://www.academia.edu/Documents/in/Fluorescence_Lifetime?f_ri=3091"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=3091"},{"id":328449,"name":"Molecules","url":"https://www.academia.edu/Documents/in/Molecules?f_ri=3091"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=3091"},{"id":838973,"name":"System performance","url":"https://www.academia.edu/Documents/in/System_performance?f_ri=3091"},{"id":907382,"name":"Digital Image","url":"https://www.academia.edu/Documents/in/Digital_Image?f_ri=3091"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=3091"},{"id":1232950,"name":"Fluorometry","url":"https://www.academia.edu/Documents/in/Fluorometry?f_ri=3091"},{"id":1311892,"name":"Fluorescence Lifetime Imaging","url":"https://www.academia.edu/Documents/in/Fluorescence_Lifetime_Imaging?f_ri=3091"},{"id":1437495,"name":"Phase Shift","url":"https://www.academia.edu/Documents/in/Phase_Shift?f_ri=3091"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091"},{"id":2307490,"name":"Expected Value","url":"https://www.academia.edu/Documents/in/Expected_Value?f_ri=3091"},{"id":2493561,"name":"Quantum Noise","url":"https://www.academia.edu/Documents/in/Quantum_Noise?f_ri=3091"},{"id":3276666,"name":"fluorescent dyes","url":"https://www.academia.edu/Documents/in/fluorescent_dyes?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_48087522" data-work_id="48087522" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48087522/Time_correlated_single_photon_counting_fluorescence_lifetime_confocal_imaging_of_decayed_and_sound_dental_structures_with_a_white_light_supercontinuum_source">Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We report the demonstration of time-correlated single-photon counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex vivo decayed and healthy dentinal tooth structures, using a white-light supercontinuum excitation source. By using a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48087522" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We report the demonstration of time-correlated single-photon counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex vivo decayed and healthy dentinal tooth structures, using a white-light supercontinuum excitation source. By using a 100 fs-pulsed Ti:Sapphire laser with a low-frequency chirp to pump a 30-cm long section of photonic crystal fibre, a ps-pulsed white-light supercontinuum was created. Optical bandpass interference filters were then applied to this broad-bandwidth source to select the 488-nm excitation wavelength required to perform TCSPC FLIM of dental structures. Decayed dentine showed significantly shorter lifetimes, discriminating it from healthy tissue and hard, stained and thus affected but noninfected material. The white-light generation source provides a flexible method of producing variable-bandwidth visible and ps-pulsed light for TCSPC FLIM. The results from the dental tissue indicate a potential method of discriminating diseased tissue from sound, but stained tissue, which could be of crucial importance in limiting tissue resection during preparation for clinical restorations.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48087522" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d8ac1b42d647d80acf7d7695d47d4ec2" rel="nofollow" data-download="{"attachment_id":66900353,"asset_id":48087522,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/66900353/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32947999" href="https://independent.academia.edu/TonyNg16">Tony Ng</a><script data-card-contents-for-user="32947999" type="text/json">{"id":32947999,"first_name":"Tony","last_name":"Ng","domain_name":"independent","page_name":"TonyNg16","display_name":"Tony Ng","profile_url":"https://independent.academia.edu/TonyNg16?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_48087522 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48087522"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48087522, container: ".js-paper-rank-work_48087522", }); });</script></li><li class="js-percentile-work_48087522 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 48087522; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_48087522"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_48087522 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="48087522"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 48087522; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=48087522]").text(description); $(".js-view-count-work_48087522").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_48087522").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="48087522"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="56" rel="nofollow" href="https://www.academia.edu/Documents/in/Materials_Engineering">Materials Engineering</a>, <script data-card-contents-for-ri="56" type="text/json">{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="505" rel="nofollow" href="https://www.academia.edu/Documents/in/Condensed_Matter_Physics">Condensed Matter Physics</a>, <script data-card-contents-for-ri="505" type="text/json">{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7700" rel="nofollow" href="https://www.academia.edu/Documents/in/Fluorescence_Microscopy">Fluorescence Microscopy</a><script data-card-contents-for-ri="7700" type="text/json">{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48087522]'), work: {"id":48087522,"title":"Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source","created_at":"2021-05-04T00:44:18.814-07:00","url":"https://www.academia.edu/48087522/Time_correlated_single_photon_counting_fluorescence_lifetime_confocal_imaging_of_decayed_and_sound_dental_structures_with_a_white_light_supercontinuum_source?f_ri=3091","dom_id":"work_48087522","summary":"We report the demonstration of time-correlated single-photon counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex vivo decayed and healthy dentinal tooth structures, using a white-light supercontinuum excitation source. By using a 100 fs-pulsed Ti:Sapphire laser with a low-frequency chirp to pump a 30-cm long section of photonic crystal fibre, a ps-pulsed white-light supercontinuum was created. Optical bandpass interference filters were then applied to this broad-bandwidth source to select the 488-nm excitation wavelength required to perform TCSPC FLIM of dental structures. Decayed dentine showed significantly shorter lifetimes, discriminating it from healthy tissue and hard, stained and thus affected but noninfected material. The white-light generation source provides a flexible method of producing variable-bandwidth visible and ps-pulsed light for TCSPC FLIM. The results from the dental tissue indicate a potential method of discriminating diseased tissue from sound, but stained tissue, which could be of crucial importance in limiting tissue resection during preparation for clinical restorations.","downloadable_attachments":[{"id":66900353,"asset_id":48087522,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32947999,"first_name":"Tony","last_name":"Ng","domain_name":"independent","page_name":"TonyNg16","display_name":"Tony Ng","profile_url":"https://independent.academia.edu/TonyNg16?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy?f_ri=3091","nofollow":true},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=3091"},{"id":39857,"name":"Molecular Imaging","url":"https://www.academia.edu/Documents/in/Molecular_Imaging?f_ri=3091"},{"id":55547,"name":"Fluorescence Lifetime","url":"https://www.academia.edu/Documents/in/Fluorescence_Lifetime?f_ri=3091"},{"id":105826,"name":"Dental Caries","url":"https://www.academia.edu/Documents/in/Dental_Caries?f_ri=3091"},{"id":162813,"name":"Fluorescence Imaging","url":"https://www.academia.edu/Documents/in/Fluorescence_Imaging?f_ri=3091"},{"id":548472,"name":"Optics and Photonics","url":"https://www.academia.edu/Documents/in/Optics_and_Photonics?f_ri=3091"},{"id":604200,"name":"Spatial resolution","url":"https://www.academia.edu/Documents/in/Spatial_resolution?f_ri=3091"},{"id":717129,"name":"Energy Transfer","url":"https://www.academia.edu/Documents/in/Energy_Transfer?f_ri=3091"},{"id":1127444,"name":"High Spatial Resolution","url":"https://www.academia.edu/Documents/in/High_Spatial_Resolution?f_ri=3091"},{"id":1145520,"name":"Equipment Design","url":"https://www.academia.edu/Documents/in/Equipment_Design?f_ri=3091"},{"id":1311892,"name":"Fluorescence Lifetime Imaging","url":"https://www.academia.edu/Documents/in/Fluorescence_Lifetime_Imaging?f_ri=3091"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091"},{"id":1985814,"name":"Near Field","url":"https://www.academia.edu/Documents/in/Near_Field?f_ri=3091"},{"id":2412368,"name":"Fluorescence quenching","url":"https://www.academia.edu/Documents/in/Fluorescence_quenching?f_ri=3091"},{"id":2868791,"name":"Distance Scale","url":"https://www.academia.edu/Documents/in/Distance_Scale?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_45457509" data-work_id="45457509" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/45457509/Degradation_of_polyurethane_gastrostomy_devices_what_is_the_role_of_fungal_colonization">Degradation of polyurethane gastrostomy devices: what is the role of fungal colonization?</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The aim of this study was to evaluate polyurethane percutaneous endoscopic gastrostomy (PEG) tube degradation and the role played by fungi. The inner surfaces of 20 used polyurethane tubes were brushed, and the brushing end was incubated... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_45457509" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The aim of this study was to evaluate polyurethane percutaneous endoscopic gastrostomy (PEG) tube degradation and the role played by fungi. The inner surfaces of 20 used polyurethane tubes were brushed, and the brushing end was incubated for 7 days in Saburaud broth and cultured if fungal growth occurred. Three tubes used for 12 (sample 12w), 17 (sample 17w), and 96 (sample 96w) weeks and two new tubes were cut to produce several 4-cm-long equal halves. Six samples from the new tubes were considered control samples (Co sample), seven were incubated in Saburaud broth (Co sample + Sa.), and seven in the broth supplemented with Candida albicans (Co sample + Sa. + Ca). All samples underwent morphological examination by electron microscopy and differential scanning calorimetry measurements (DSC). All tubes had fungal colonization. DSC showed deterioration in all tubes including the new ones; adding Candida albicans had no additional effects. Morphological examination by electron microscopy showed a regular pattern in the Co sample, and thick biofilm, holes, and crevices in samples 12w, 17w, and 96w. The more the tubes had been used, the more severe were the changes. The Co sample + Sa and the Co sample + Sa + Ca showed no changes in the inner surface, but cryogenically fractured surfaces had holes and crevices. Yeasts constantly colonize PEG tubes and are likely to contribute to polyurethane deterioration. The impairment of new PEG tubes incubated in Saburaud broth suggests that other factors also play a role in polyurethane deterioration.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/45457509" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1c78b2d38b683dbc0a718cafcef7f163" rel="nofollow" data-download="{"attachment_id":65964270,"asset_id":45457509,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/65964270/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="94659648" href="https://independent.academia.edu/MarcoScoponi">Marco Scoponi</a><script data-card-contents-for-user="94659648" type="text/json">{"id":94659648,"first_name":"Marco","last_name":"Scoponi","domain_name":"independent","page_name":"MarcoScoponi","display_name":"Marco Scoponi","profile_url":"https://independent.academia.edu/MarcoScoponi?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_45457509 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="45457509"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 45457509, container: ".js-paper-rank-work_45457509", }); });</script></li><li class="js-percentile-work_45457509 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 45457509; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_45457509"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_45457509 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="45457509"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 45457509; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=45457509]").text(description); $(".js-view-count-work_45457509").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_45457509").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="45457509"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2184" rel="nofollow" href="https://www.academia.edu/Documents/in/Electron_Microscopy">Electron Microscopy</a>, <script data-card-contents-for-ri="2184" type="text/json">{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="48303" rel="nofollow" href="https://www.academia.edu/Documents/in/Colonization">Colonization</a>, <script data-card-contents-for-ri="48303" type="text/json">{"id":48303,"name":"Colonization","url":"https://www.academia.edu/Documents/in/Colonization?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="78753" rel="nofollow" href="https://www.academia.edu/Documents/in/Differential_scanning_calorimetry">Differential scanning calorimetry</a><script data-card-contents-for-ri="78753" type="text/json">{"id":78753,"name":"Differential scanning calorimetry","url":"https://www.academia.edu/Documents/in/Differential_scanning_calorimetry?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=45457509]'), work: {"id":45457509,"title":"Degradation of polyurethane gastrostomy devices: what is the role of fungal colonization?","created_at":"2021-03-10T10:46:12.906-08:00","url":"https://www.academia.edu/45457509/Degradation_of_polyurethane_gastrostomy_devices_what_is_the_role_of_fungal_colonization?f_ri=3091","dom_id":"work_45457509","summary":"The aim of this study was to evaluate polyurethane percutaneous endoscopic gastrostomy (PEG) tube degradation and the role played by fungi. The inner surfaces of 20 used polyurethane tubes were brushed, and the brushing end was incubated for 7 days in Saburaud broth and cultured if fungal growth occurred. Three tubes used for 12 (sample 12w), 17 (sample 17w), and 96 (sample 96w) weeks and two new tubes were cut to produce several 4-cm-long equal halves. Six samples from the new tubes were considered control samples (Co sample), seven were incubated in Saburaud broth (Co sample + Sa.), and seven in the broth supplemented with Candida albicans (Co sample + Sa. + Ca). All samples underwent morphological examination by electron microscopy and differential scanning calorimetry measurements (DSC). All tubes had fungal colonization. DSC showed deterioration in all tubes including the new ones; adding Candida albicans had no additional effects. Morphological examination by electron microscopy showed a regular pattern in the Co sample, and thick biofilm, holes, and crevices in samples 12w, 17w, and 96w. The more the tubes had been used, the more severe were the changes. The Co sample + Sa and the Co sample + Sa + Ca showed no changes in the inner surface, but cryogenically fractured surfaces had holes and crevices. Yeasts constantly colonize PEG tubes and are likely to contribute to polyurethane deterioration. The impairment of new PEG tubes incubated in Saburaud broth suggests that other factors also play a role in polyurethane deterioration.","downloadable_attachments":[{"id":65964270,"asset_id":45457509,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":94659648,"first_name":"Marco","last_name":"Scoponi","domain_name":"independent","page_name":"MarcoScoponi","display_name":"Marco Scoponi","profile_url":"https://independent.academia.edu/MarcoScoponi?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":48303,"name":"Colonization","url":"https://www.academia.edu/Documents/in/Colonization?f_ri=3091","nofollow":true},{"id":78753,"name":"Differential scanning calorimetry","url":"https://www.academia.edu/Documents/in/Differential_scanning_calorimetry?f_ri=3091","nofollow":true},{"id":79747,"name":"Colonisation","url":"https://www.academia.edu/Documents/in/Colonisation?f_ri=3091"},{"id":105582,"name":"Candida","url":"https://www.academia.edu/Documents/in/Candida?f_ri=3091"},{"id":118104,"name":"Electron","url":"https://www.academia.edu/Documents/in/Electron?f_ri=3091"},{"id":131573,"name":"Candida albicans","url":"https://www.academia.edu/Documents/in/Candida_albicans?f_ri=3091"},{"id":163878,"name":"Degradation","url":"https://www.academia.edu/Documents/in/Degradation?f_ri=3091"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=3091"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=3091"},{"id":460006,"name":"Polyurethanes","url":"https://www.academia.edu/Documents/in/Polyurethanes?f_ri=3091"},{"id":869579,"name":"Gastrostomy","url":"https://www.academia.edu/Documents/in/Gastrostomy?f_ri=3091"},{"id":1758627,"name":"Enteral Nutrition","url":"https://www.academia.edu/Documents/in/Enteral_Nutrition?f_ri=3091"},{"id":2837323,"name":"Equipment failure","url":"https://www.academia.edu/Documents/in/Equipment_failure?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_42765612 coauthored" data-work_id="42765612" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/42765612/Controlling_the_morphology_of_TiO2_nanocrystals_with_different_capping_agents">Controlling the morphology of TiO2 nanocrystals with different capping agents</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This paper provides direct evidence to support the role of capping agents in controlling the evolution of TiO2 seeds into nanocrystals with a specific shape. Starting with Ti(OBut) 4 and using oleid acid, oleylamine, dioleamide,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_42765612" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This paper provides direct evidence to support the role of capping agents in controlling the evolution of TiO2 seeds into nanocrystals with a specific shape. Starting with Ti(OBut) 4 and using oleid acid, oleylamine, dioleamide, 11-aminoundecanoic acid, arginine, trifluroacetic acid or HF as capping agents, mainly TiO 2 truncated octahedrons enclosed by {1 0 1} and {0 0 1} facets were obtained. We could also selectively obtain square, rods and rounded rhombic-shaped nanoparticles by growing of {0 1 0} facets by adding oleic acid and oleyl-amine in ratio 6:4, respectively, while all other parameters were kept the same. This research not only offers new insights into the role played by a capping agent in shape-controlled synthesis but also provides, a versatile approach to controlling the shape of metal oxide nanocrystals.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/42765612" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4a7e678e707bce9e0fd185eaa7a2f5eb" rel="nofollow" data-download="{"attachment_id":62987872,"asset_id":42765612,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/62987872/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="105507567" href="https://csic.academia.edu/DavidGonzalezCalatayud">David Gonzalez Calatayud</a><script data-card-contents-for-user="105507567" type="text/json">{"id":105507567,"first_name":"David Gonzalez","last_name":"Calatayud","domain_name":"csic","page_name":"DavidGonzalezCalatayud","display_name":"David Gonzalez Calatayud","profile_url":"https://csic.academia.edu/DavidGonzalezCalatayud?f_ri=3091","photo":"https://0.academia-photos.com/105507567/24126478/23092374/s65_david_gonzalez.calatayud.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-42765612">+1</span><div class="hidden js-additional-users-42765612"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/AmadorCaballero1">Amador Caballero</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-42765612'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-42765612').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_42765612 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="42765612"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 42765612, container: ".js-paper-rank-work_42765612", }); });</script></li><li class="js-percentile-work_42765612 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 42765612; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_42765612"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_42765612 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="42765612"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42765612; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42765612]").text(description); $(".js-view-count-work_42765612").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_42765612").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="42765612"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">4</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6400" rel="nofollow" href="https://www.academia.edu/Documents/in/Photocatalysts">Photocatalysts</a>, <script data-card-contents-for-ri="6400" type="text/json">{"id":6400,"name":"Photocatalysts","url":"https://www.academia.edu/Documents/in/Photocatalysts?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="13621" rel="nofollow" href="https://www.academia.edu/Documents/in/Nanoparticles">Nanoparticles</a>, <script data-card-contents-for-ri="13621" type="text/json">{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="143739" rel="nofollow" href="https://www.academia.edu/Documents/in/TiO2">TiO2</a><script data-card-contents-for-ri="143739" type="text/json">{"id":143739,"name":"TiO2","url":"https://www.academia.edu/Documents/in/TiO2?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=42765612]'), work: {"id":42765612,"title":"Controlling the morphology of TiO2 nanocrystals with different capping agents","created_at":"2020-04-17T04:55:59.013-07:00","url":"https://www.academia.edu/42765612/Controlling_the_morphology_of_TiO2_nanocrystals_with_different_capping_agents?f_ri=3091","dom_id":"work_42765612","summary":"This paper provides direct evidence to support the role of capping agents in controlling the evolution of TiO2 seeds into nanocrystals with a specific shape. Starting with Ti(OBut) 4 and using oleid acid, oleylamine, dioleamide, 11-aminoundecanoic acid, arginine, trifluroacetic acid or HF as capping agents, mainly TiO 2 truncated octahedrons enclosed by {1 0 1} and {0 0 1} facets were obtained. We could also selectively obtain square, rods and rounded rhombic-shaped nanoparticles by growing of {0 1 0} facets by adding oleic acid and oleyl-amine in ratio 6:4, respectively, while all other parameters were kept the same. This research not only offers new insights into the role played by a capping agent in shape-controlled synthesis but also provides, a versatile approach to controlling the shape of metal oxide nanocrystals.","downloadable_attachments":[{"id":62987872,"asset_id":42765612,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":105507567,"first_name":"David Gonzalez","last_name":"Calatayud","domain_name":"csic","page_name":"DavidGonzalezCalatayud","display_name":"David Gonzalez Calatayud","profile_url":"https://csic.academia.edu/DavidGonzalezCalatayud?f_ri=3091","photo":"https://0.academia-photos.com/105507567/24126478/23092374/s65_david_gonzalez.calatayud.jpg"},{"id":59159047,"first_name":"Amador","last_name":"Caballero","domain_name":"independent","page_name":"AmadorCaballero1","display_name":"Amador Caballero","profile_url":"https://independent.academia.edu/AmadorCaballero1?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":6400,"name":"Photocatalysts","url":"https://www.academia.edu/Documents/in/Photocatalysts?f_ri=3091","nofollow":true},{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles?f_ri=3091","nofollow":true},{"id":143739,"name":"TiO2","url":"https://www.academia.edu/Documents/in/TiO2?f_ri=3091","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_40799483" data-work_id="40799483" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/40799483/Clinical_accuracy_of_a_new_apex_locator_with_an_automatic_compensation_circuit">Clinical accuracy of a new apex locator with an automatic compensation circuit</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A new circuit was designed to automatically compensate for measurement errors of an electronic apex locator in various electrolytes. Thirty-one root canals were clinically tested for accuracy. A file was inserted into the canal until the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_40799483" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A new circuit was designed to automatically compensate for measurement errors of an electronic apex locator in various electrolytes. Thirty-one root canals were clinically tested for accuracy. A file was inserted into the canal until the apex signal was obtained, at which point the file was immobilized with glass-ionomer cement. After extraction, the apical area was exposed and the position of the file tip was examined under an operating microscope. Distances from the major foramen and cemento dentinal junction (CDJ) were recorded. The average distance from the major foramen was -0.13 mm with a range of -1.28 mm and +0.46 mm. The average distance in 26 detectable CDJ samples was +0.18 mm with a range of -0.98 mm and +0.65 mm. The measurements, which were within +/- 0.5 mm, were 94% (29/31) from the major foramen and 92% (24/26) from the CDJ. Measurement consistencies within one SD were 81% for the major foramen and 65% for the CDJ, respectively. Measurements within two SD were 97% f...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/40799483" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2bdb22a4ca46a263e7cf9c3542e621cb" rel="nofollow" data-download="{"attachment_id":61082346,"asset_id":40799483,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/61082346/download_file?st=MTc0MDY0ODAyNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="133436470" href="https://independent.academia.edu/deoksoonkim">deoksoon kim</a><script data-card-contents-for-user="133436470" type="text/json">{"id":133436470,"first_name":"deoksoon","last_name":"kim","domain_name":"independent","page_name":"deoksoonkim","display_name":"deoksoon kim","profile_url":"https://independent.academia.edu/deoksoonkim?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_40799483 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="40799483"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 40799483, container: ".js-paper-rank-work_40799483", }); });</script></li><li class="js-percentile-work_40799483 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 40799483; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_40799483"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_40799483 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="40799483"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 40799483; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=40799483]").text(description); $(".js-view-count-work_40799483").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_40799483").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="40799483"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="596" rel="nofollow" href="https://www.academia.edu/Documents/in/Dentistry">Dentistry</a>, <script data-card-contents-for-ri="596" type="text/json">{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4758" rel="nofollow" href="https://www.academia.edu/Documents/in/Electronics">Electronics</a>, <script data-card-contents-for-ri="4758" type="text/json">{"id":4758,"name":"Electronics","url":"https://www.academia.edu/Documents/in/Electronics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="274826" rel="nofollow" href="https://www.academia.edu/Documents/in/Hydrogen_Peroxide">Hydrogen Peroxide</a><script data-card-contents-for-ri="274826" type="text/json">{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=40799483]'), work: {"id":40799483,"title":"Clinical accuracy of a new apex locator with an automatic compensation circuit","created_at":"2019-10-31T12:36:17.844-07:00","url":"https://www.academia.edu/40799483/Clinical_accuracy_of_a_new_apex_locator_with_an_automatic_compensation_circuit?f_ri=3091","dom_id":"work_40799483","summary":"A new circuit was designed to automatically compensate for measurement errors of an electronic apex locator in various electrolytes. Thirty-one root canals were clinically tested for accuracy. A file was inserted into the canal until the apex signal was obtained, at which point the file was immobilized with glass-ionomer cement. After extraction, the apical area was exposed and the position of the file tip was examined under an operating microscope. Distances from the major foramen and cemento dentinal junction (CDJ) were recorded. The average distance from the major foramen was -0.13 mm with a range of -1.28 mm and +0.46 mm. The average distance in 26 detectable CDJ samples was +0.18 mm with a range of -0.98 mm and +0.65 mm. The measurements, which were within +/- 0.5 mm, were 94% (29/31) from the major foramen and 92% (24/26) from the CDJ. Measurement consistencies within one SD were 81% for the major foramen and 65% for the CDJ, respectively. Measurements within two SD were 97% f...","downloadable_attachments":[{"id":61082346,"asset_id":40799483,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":133436470,"first_name":"deoksoon","last_name":"kim","domain_name":"independent","page_name":"deoksoonkim","display_name":"deoksoon kim","profile_url":"https://independent.academia.edu/deoksoonkim?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":4758,"name":"Electronics","url":"https://www.academia.edu/Documents/in/Electronics?f_ri=3091","nofollow":true},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide?f_ri=3091","nofollow":true},{"id":342520,"name":"Sodium Hypochlorite","url":"https://www.academia.edu/Documents/in/Sodium_Hypochlorite?f_ri=3091"},{"id":372652,"name":"Endodontics","url":"https://www.academia.edu/Documents/in/Endodontics?f_ri=3091"},{"id":373249,"name":"Electrolytes","url":"https://www.academia.edu/Documents/in/Electrolytes?f_ri=3091"},{"id":375296,"name":"Observer Variation","url":"https://www.academia.edu/Documents/in/Observer_Variation?f_ri=3091"},{"id":413190,"name":"Odontometry","url":"https://www.academia.edu/Documents/in/Odontometry?f_ri=3091"},{"id":558068,"name":"Dentin","url":"https://www.academia.edu/Documents/in/Dentin?f_ri=3091"},{"id":685379,"name":"DENTAL CEMENTUM","url":"https://www.academia.edu/Documents/in/DENTAL_CEMENTUM?f_ri=3091"},{"id":892969,"name":"Materials Testing","url":"https://www.academia.edu/Documents/in/Materials_Testing?f_ri=3091"},{"id":1145520,"name":"Equipment Design","url":"https://www.academia.edu/Documents/in/Equipment_Design?f_ri=3091"},{"id":1223913,"name":"Sodium Chloride","url":"https://www.academia.edu/Documents/in/Sodium_Chloride?f_ri=3091"},{"id":1272890,"name":"Electric Impedance","url":"https://www.academia.edu/Documents/in/Electric_Impedance?f_ri=3091"},{"id":2463496,"name":"Statistics as Topic","url":"https://www.academia.edu/Documents/in/Statistics_as_Topic?f_ri=3091"},{"id":2578012,"name":"root canal preparation","url":"https://www.academia.edu/Documents/in/root_canal_preparation?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_34015935 coauthored" data-work_id="34015935" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/34015935/Qualitative_evaluation_of_tortellini_meat_filling_by_histology_and_image_analysis">Qualitative evaluation of tortellini meat filling by histology and image analysis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The aim of this study was to identify the presence of different animal tissues in tortellini meat-filling with special emphasis on the percentage of skeletal muscle. Industrially packed and refrigerated tortellini filling from four... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_34015935" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The aim of this study was to identify the presence of different animal tissues in tortellini meat-filling with special emphasis on the percentage of skeletal muscle. Industrially packed and refrigerated tortellini filling from four different Italian commercial brands were examined and compared. From each brand, five different batches were sampled at intervals of 1 month. From each batch, four fillings were randomly sampled and coded to allow a blind examination. Histological sections were examined to identify the presence of various animal tissues. The presence of large fragments of skeletal muscle in specimens were assessed and semiquantitatively ranked. To evaluate the percentage area of skeletal muscle in the samples, a computerised image analysis system was used. Animal tissues were well preserved in all examined samples. The filling quality was quantified by histology and image analysis evaluating: skeletal muscle density, size of skeletal muscle fragments, and uniformity of batches. In addition to the effective enforcement of food labelling law and the protection of consumers, histology and image analysis are reliable tools to identify small amounts of various animal tissues in a processed meat product.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/34015935" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9ab5a34ba3394781a84127936ee272f7" rel="nofollow" data-download="{"attachment_id":53960286,"asset_id":34015935,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/53960286/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="66675724" href="https://independent.academia.edu/SimoneStella4">Simone Stella</a><script data-card-contents-for-user="66675724" type="text/json">{"id":66675724,"first_name":"Simone","last_name":"Stella","domain_name":"independent","page_name":"SimoneStella4","display_name":"Simone Stella","profile_url":"https://independent.academia.edu/SimoneStella4?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-34015935">+1</span><div class="hidden js-additional-users-34015935"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/SilvanaMattiello">Silvana Mattiello</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-34015935'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-34015935').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_34015935 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="34015935"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 34015935, container: ".js-paper-rank-work_34015935", }); });</script></li><li class="js-percentile-work_34015935 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34015935; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_34015935"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_34015935 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="34015935"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34015935; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34015935]").text(description); $(".js-view-count-work_34015935").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_34015935").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="34015935"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="8910" rel="nofollow" href="https://www.academia.edu/Documents/in/Evaluation">Evaluation</a>, <script data-card-contents-for-ri="8910" type="text/json">{"id":8910,"name":"Evaluation","url":"https://www.academia.edu/Documents/in/Evaluation?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9351" rel="nofollow" href="https://www.academia.edu/Documents/in/Image_Analysis">Image Analysis</a>, <script data-card-contents-for-ri="9351" type="text/json">{"id":9351,"name":"Image Analysis","url":"https://www.academia.edu/Documents/in/Image_Analysis?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12069" rel="nofollow" href="https://www.academia.edu/Documents/in/Histology">Histology</a><script data-card-contents-for-ri="12069" type="text/json">{"id":12069,"name":"Histology","url":"https://www.academia.edu/Documents/in/Histology?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=34015935]'), work: {"id":34015935,"title":"Qualitative evaluation of tortellini meat filling by histology and image analysis","created_at":"2017-07-24T03:43:44.190-07:00","url":"https://www.academia.edu/34015935/Qualitative_evaluation_of_tortellini_meat_filling_by_histology_and_image_analysis?f_ri=3091","dom_id":"work_34015935","summary":"The aim of this study was to identify the presence of different animal tissues in tortellini meat-filling with special emphasis on the percentage of skeletal muscle. Industrially packed and refrigerated tortellini filling from four different Italian commercial brands were examined and compared. From each brand, five different batches were sampled at intervals of 1 month. From each batch, four fillings were randomly sampled and coded to allow a blind examination. Histological sections were examined to identify the presence of various animal tissues. The presence of large fragments of skeletal muscle in specimens were assessed and semiquantitatively ranked. To evaluate the percentage area of skeletal muscle in the samples, a computerised image analysis system was used. Animal tissues were well preserved in all examined samples. The filling quality was quantified by histology and image analysis evaluating: skeletal muscle density, size of skeletal muscle fragments, and uniformity of batches. In addition to the effective enforcement of food labelling law and the protection of consumers, histology and image analysis are reliable tools to identify small amounts of various animal tissues in a processed meat product.","downloadable_attachments":[{"id":53960286,"asset_id":34015935,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":66675724,"first_name":"Simone","last_name":"Stella","domain_name":"independent","page_name":"SimoneStella4","display_name":"Simone Stella","profile_url":"https://independent.academia.edu/SimoneStella4?f_ri=3091","photo":"/images/s65_no_pic.png"},{"id":37523833,"first_name":"Silvana","last_name":"Mattiello","domain_name":"independent","page_name":"SilvanaMattiello","display_name":"Silvana Mattiello","profile_url":"https://independent.academia.edu/SilvanaMattiello?f_ri=3091","photo":"https://0.academia-photos.com/37523833/118698052/108011205/s65_silvana.mattiello.jpeg"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":8910,"name":"Evaluation","url":"https://www.academia.edu/Documents/in/Evaluation?f_ri=3091","nofollow":true},{"id":9351,"name":"Image Analysis","url":"https://www.academia.edu/Documents/in/Image_Analysis?f_ri=3091","nofollow":true},{"id":12069,"name":"Histology","url":"https://www.academia.edu/Documents/in/Histology?f_ri=3091","nofollow":true},{"id":34478,"name":"Food Science and Technology","url":"https://www.academia.edu/Documents/in/Food_Science_and_Technology?f_ri=3091"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_31345949" data-work_id="31345949" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/31345949/Rotational_Reorganization_of_Doped_Cholesteric_Liquid_Crystalline_Films">Rotational Reorganization of Doped Cholesteric Liquid Crystalline Films</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">In this paper an unprecedented rotational reorganization of cholesteric liquid crystalline films is described. This rotational reorganization results from the conversion of a chiral molecular motor dopant to an isomer with a different... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_31345949" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In this paper an unprecedented rotational reorganization of cholesteric liquid crystalline films is described. This rotational reorganization results from the conversion of a chiral molecular motor dopant to an isomer with a different helical twisting power, leading to a change in the cholesteric pitch. The direction of this reorganization is correlated to the sign of the change in helical twisting power of the dopant. The rotational reorganization of the liquid crystalline film was used to rotate microscopic objects 4 orders of magnitude larger than the bistable dopants in the film, which shows that molecular motors and switches can perform work. The surface of the doped cholesteric liquid crystalline films was found to possess a regular surface relief, whose periodicity coincides with typical cholesteric polygonal line textures. These surface features originate from the cholesteric superstructure in the liquid crystalline film, which in turn is the result of the presence of the chiral dopant. As such, the presence of the dopant is expressed in these distinct surface structures. A possible mechanism at the origin of the rotational reorganization of liquid crystalline films and the cholesteric surface relief is discussed.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/31345949" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="73c7a89babfeca4b8555c1d95ea11335" rel="nofollow" data-download="{"attachment_id":51729028,"asset_id":31345949,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/51729028/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="59906715" href="https://ehu.academia.edu/JavierVicario">Javier Vicario</a><script data-card-contents-for-user="59906715" type="text/json">{"id":59906715,"first_name":"Javier","last_name":"Vicario","domain_name":"ehu","page_name":"JavierVicario","display_name":"Javier Vicario","profile_url":"https://ehu.academia.edu/JavierVicario?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_31345949 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="31345949"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 31345949, container: ".js-paper-rank-work_31345949", }); });</script></li><li class="js-percentile-work_31345949 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 31345949; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_31345949"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_31345949 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="31345949"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 31345949; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=31345949]").text(description); $(".js-view-count-work_31345949").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_31345949").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="31345949"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="522" rel="nofollow" href="https://www.academia.edu/Documents/in/Thermodynamics">Thermodynamics</a>, <script data-card-contents-for-ri="522" type="text/json">{"id":522,"name":"Thermodynamics","url":"https://www.academia.edu/Documents/in/Thermodynamics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5020" rel="nofollow" href="https://www.academia.edu/Documents/in/Liquid_Crystals">Liquid Crystals</a>, <script data-card-contents-for-ri="5020" type="text/json">{"id":5020,"name":"Liquid Crystals","url":"https://www.academia.edu/Documents/in/Liquid_Crystals?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12597" rel="nofollow" href="https://www.academia.edu/Documents/in/Crystallization">Crystallization</a><script data-card-contents-for-ri="12597" type="text/json">{"id":12597,"name":"Crystallization","url":"https://www.academia.edu/Documents/in/Crystallization?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=31345949]'), work: {"id":31345949,"title":"Rotational Reorganization of Doped Cholesteric Liquid Crystalline Films","created_at":"2017-02-10T01:51:39.261-08:00","url":"https://www.academia.edu/31345949/Rotational_Reorganization_of_Doped_Cholesteric_Liquid_Crystalline_Films?f_ri=3091","dom_id":"work_31345949","summary":"In this paper an unprecedented rotational reorganization of cholesteric liquid crystalline films is described. This rotational reorganization results from the conversion of a chiral molecular motor dopant to an isomer with a different helical twisting power, leading to a change in the cholesteric pitch. The direction of this reorganization is correlated to the sign of the change in helical twisting power of the dopant. The rotational reorganization of the liquid crystalline film was used to rotate microscopic objects 4 orders of magnitude larger than the bistable dopants in the film, which shows that molecular motors and switches can perform work. The surface of the doped cholesteric liquid crystalline films was found to possess a regular surface relief, whose periodicity coincides with typical cholesteric polygonal line textures. These surface features originate from the cholesteric superstructure in the liquid crystalline film, which in turn is the result of the presence of the chiral dopant. As such, the presence of the dopant is expressed in these distinct surface structures. A possible mechanism at the origin of the rotational reorganization of liquid crystalline films and the cholesteric surface relief is discussed.","downloadable_attachments":[{"id":51729028,"asset_id":31345949,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":59906715,"first_name":"Javier","last_name":"Vicario","domain_name":"ehu","page_name":"JavierVicario","display_name":"Javier Vicario","profile_url":"https://ehu.academia.edu/JavierVicario?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":522,"name":"Thermodynamics","url":"https://www.academia.edu/Documents/in/Thermodynamics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":5020,"name":"Liquid Crystals","url":"https://www.academia.edu/Documents/in/Liquid_Crystals?f_ri=3091","nofollow":true},{"id":12597,"name":"Crystallization","url":"https://www.academia.edu/Documents/in/Crystallization?f_ri=3091","nofollow":true},{"id":161176,"name":"The","url":"https://www.academia.edu/Documents/in/The?f_ri=3091"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=3091"},{"id":440924,"name":"Surface Properties","url":"https://www.academia.edu/Documents/in/Surface_Properties?f_ri=3091"},{"id":818658,"name":"Rotation","url":"https://www.academia.edu/Documents/in/Rotation?f_ri=3091"},{"id":1724844,"name":"Molecular Structure","url":"https://www.academia.edu/Documents/in/Molecular_Structure?f_ri=3091"},{"id":1958280,"name":"Liquid Crystalline","url":"https://www.academia.edu/Documents/in/Liquid_Crystalline?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3515522 coauthored" data-work_id="3515522" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3515522/Characterization_of_cerium_oxide_nanoparticles_part_2_nonsize_measurements">Characterization of cerium oxide nanoparticles-part 2: nonsize measurements</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The present study gives an overview of some of the major aspects for consideration in the characterization of nanomaterials (NMs). Part 1 focuses on the measurement of particle size and size-related parameters using several analytical... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_3515522" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The present study gives an overview of some of the major aspects for consideration in the characterization of nanomaterials (NMs). Part 1 focuses on the measurement of particle size and size-related parameters using several analytical techniques such as transmission electron microscopy, atomic force microscopy, dynamic light scattering, X-ray diffraction, and Brunauer, Emmett, and Teller surface area measurements as applied to commercially available cerium oxide nanoparticles (NPs) and microparticles (MPs). Part 2 (see companion paper) considers nonsize-related characterization and analysis. The results are discussed in relation to the nature of the sample and preparation, and the analytical principles, limitations, and advantages of each technique. Accurate information on the particle size of the different fractions of a sample can be obtained by using a combination of different types of microscopy, spectroscopy, separation, and other techniques; this should inform ecotoxicological and environmental studies. The good agreement between the measured primary particle size of the NPs ($15 nm) by atomic force microscopy, transmission electron microscopy, X-ray diffraction, and Brunauer, Emmett, and Teller suggests that the primary particles are formed of semispherical single crystals. For MPs, all measurements agree that they are large particles in the range above the NPs (100 nm), with some difference between the measured sizes, possibly as a result of polydispersity effects. Additionally, our findings suggest that atomic force microscopy and transmission electron microscopy prepared by centrifugation methods provide consistent data at low concentrations when dynamic light scattering fails. Environ. Toxicol. Chem. 2012;31:983-993. # 2012 SETAC</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3515522" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="de5344de9ebe8b21ab1a3e99ab1baf6f" rel="nofollow" data-download="{"attachment_id":50254842,"asset_id":3515522,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50254842/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="441874" href="https://psych-sc.academia.edu/MohammedBaalousha">Mohammed Baalousha</a><script data-card-contents-for-user="441874" type="text/json">{"id":441874,"first_name":"Mohammed","last_name":"Baalousha","domain_name":"psych-sc","page_name":"MohammedBaalousha","display_name":"Mohammed Baalousha","profile_url":"https://psych-sc.academia.edu/MohammedBaalousha?f_ri=3091","photo":"https://0.academia-photos.com/441874/142093/2092026/s65_mohammed.baalousha.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-3515522">+1</span><div class="hidden js-additional-users-3515522"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/VickiStone">Vicki Stone</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-3515522'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-3515522').html(); 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Part 1 focuses on the measurement of particle size and size-related parameters using several analytical techniques such as transmission electron microscopy, atomic force microscopy, dynamic light scattering, X-ray diffraction, and Brunauer, Emmett, and Teller surface area measurements as applied to commercially available cerium oxide nanoparticles (NPs) and microparticles (MPs). Part 2 (see companion paper) considers nonsize-related characterization and analysis. The results are discussed in relation to the nature of the sample and preparation, and the analytical principles, limitations, and advantages of each technique. Accurate information on the particle size of the different fractions of a sample can be obtained by using a combination of different types of microscopy, spectroscopy, separation, and other techniques; this should inform ecotoxicological and environmental studies. The good agreement between the measured primary particle size of the NPs ($15 nm) by atomic force microscopy, transmission electron microscopy, X-ray diffraction, and Brunauer, Emmett, and Teller suggests that the primary particles are formed of semispherical single crystals. For MPs, all measurements agree that they are large particles in the range above the NPs (100 nm), with some difference between the measured sizes, possibly as a result of polydispersity effects. Additionally, our findings suggest that atomic force microscopy and transmission electron microscopy prepared by centrifugation methods provide consistent data at low concentrations when dynamic light scattering fails. Environ. Toxicol. Chem. 2012;31:983-993. # 2012 SETAC","downloadable_attachments":[{"id":50254842,"asset_id":3515522,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":441874,"first_name":"Mohammed","last_name":"Baalousha","domain_name":"psych-sc","page_name":"MohammedBaalousha","display_name":"Mohammed Baalousha","profile_url":"https://psych-sc.academia.edu/MohammedBaalousha?f_ri=3091","photo":"https://0.academia-photos.com/441874/142093/2092026/s65_mohammed.baalousha.jpg"},{"id":37075476,"first_name":"Vicki","last_name":"Stone","domain_name":"independent","page_name":"VickiStone","display_name":"Vicki Stone","profile_url":"https://independent.academia.edu/VickiStone?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":10655,"name":"Scanning Electron Microscopy","url":"https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy?f_ri=3091","nofollow":true},{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles?f_ri=3091","nofollow":true},{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy?f_ri=3091","nofollow":true},{"id":24373,"name":"Atomic Force Microscopy","url":"https://www.academia.edu/Documents/in/Atomic_Force_Microscopy?f_ri=3091"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=3091"},{"id":50630,"name":"Crystal structure","url":"https://www.academia.edu/Documents/in/Crystal_structure?f_ri=3091"},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=3091"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=3091"},{"id":386276,"name":"Cerium","url":"https://www.academia.edu/Documents/in/Cerium?f_ri=3091"},{"id":386527,"name":"X ray diffraction","url":"https://www.academia.edu/Documents/in/X_ray_diffraction?f_ri=3091"},{"id":390245,"name":"Particle Size","url":"https://www.academia.edu/Documents/in/Particle_Size?f_ri=3091"},{"id":440924,"name":"Surface Properties","url":"https://www.academia.edu/Documents/in/Surface_Properties?f_ri=3091"},{"id":444844,"name":"Spectrum analysis","url":"https://www.academia.edu/Documents/in/Spectrum_analysis?f_ri=3091"},{"id":492043,"name":"Size","url":"https://www.academia.edu/Documents/in/Size?f_ri=3091"},{"id":882010,"name":"Environmental Toxicology and Chemistry","url":"https://www.academia.edu/Documents/in/Environmental_Toxicology_and_Chemistry?f_ri=3091"},{"id":1013858,"name":"Cations","url":"https://www.academia.edu/Documents/in/Cations?f_ri=3091"},{"id":2054788,"name":"Isoelectric point","url":"https://www.academia.edu/Documents/in/Isoelectric_point?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_12673695" data-work_id="12673695" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/12673695/Chaetomium_globosum_Cutaneous_Fungal_Infection_Confirmed_by_Molecular_Identification_A_Case_Report_from_Malaysia">Chaetomium globosum Cutaneous Fungal Infection Confirmed by Molecular Identification: A Case Report from Malaysia</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">An 11-year-old girl presented with multiple blisters on her the right foot complicated with cellulitis. The conventional and molecular identification were performed on the culture. The internal transcribed spacer (ITS) region in rRNA gene... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_12673695" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">An 11-year-old girl presented with multiple blisters on her the right foot complicated with cellulitis. The conventional and molecular identification were performed on the culture. The internal transcribed spacer (ITS) region in rRNA gene of the isolate was amplified by PCR. The sequence of the amplified ITS region matched 99 % with that of Chaetomium globosum in the GenBank. This is the first report describing C. globosum causing cutaneous infection in Malaysia.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/12673695" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a2ae47efb13420ef10791ad2dd961f0f" rel="nofollow" data-download="{"attachment_id":46014199,"asset_id":12673695,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46014199/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="31675289" href="https://independent.academia.edu/NurAhmad42">Nur Ahmad</a><script data-card-contents-for-user="31675289" type="text/json">{"id":31675289,"first_name":"Nur","last_name":"Ahmad","domain_name":"independent","page_name":"NurAhmad42","display_name":"Nur Ahmad","profile_url":"https://independent.academia.edu/NurAhmad42?f_ri=3091","photo":"https://0.academia-photos.com/31675289/9386597/10461236/s65_nur.ahmad.jpg"}</script></span></span></li><li class="js-paper-rank-work_12673695 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="12673695"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 12673695, container: ".js-paper-rank-work_12673695", }); 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The conventional and molecular identification were performed on the culture. The internal transcribed spacer (ITS) region in rRNA gene of the isolate was amplified by PCR. The sequence of the amplified ITS region matched 99 % with that of Chaetomium globosum in the GenBank. This is the first report describing C. globosum causing cutaneous infection in Malaysia.","downloadable_attachments":[{"id":46014199,"asset_id":12673695,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":31675289,"first_name":"Nur","last_name":"Ahmad","domain_name":"independent","page_name":"NurAhmad42","display_name":"Nur Ahmad","profile_url":"https://independent.academia.edu/NurAhmad42?f_ri=3091","photo":"https://0.academia-photos.com/31675289/9386597/10461236/s65_nur.ahmad.jpg"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=3091","nofollow":true},{"id":6818,"name":"Malaysia","url":"https://www.academia.edu/Documents/in/Malaysia?f_ri=3091","nofollow":true},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child?f_ri=3091","nofollow":true},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=3091"},{"id":839246,"name":"Molecular Diagnostic Techniques","url":"https://www.academia.edu/Documents/in/Molecular_Diagnostic_Techniques?f_ri=3091"},{"id":874564,"name":"Chaetomium Globosum","url":"https://www.academia.edu/Documents/in/Chaetomium_Globosum?f_ri=3091"},{"id":1724347,"name":"Cellulitis","url":"https://www.academia.edu/Documents/in/Cellulitis?f_ri=3091"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_25514895" data-work_id="25514895" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/25514895/Appendicitis_in_a_4_Year_Old_Child">Appendicitis in a 4-Year-Old Child</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/25514895" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8c24fd4c1e2a747ac6a555b1dd305971" rel="nofollow" data-download="{"attachment_id":45837423,"asset_id":25514895,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45837423/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="49050108" href="https://independent.academia.edu/BobbiPritt">Bobbi Pritt</a><script data-card-contents-for-user="49050108" type="text/json">{"id":49050108,"first_name":"Bobbi","last_name":"Pritt","domain_name":"independent","page_name":"BobbiPritt","display_name":"Bobbi Pritt","profile_url":"https://independent.academia.edu/BobbiPritt?f_ri=3091","photo":"https://gravatar.com/avatar/fba7bf2e8b2aae4e60a1dbdd6938b531?s=65"}</script></span></span></li><li class="js-paper-rank-work_25514895 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="25514895"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 25514895, container: ".js-paper-rank-work_25514895", }); 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With this very general kernel regularization term, we can stabilize the blind MLEM scheme even for... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14115469" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We propose to use a kernel intensity penalizer (KIP) in the blind maximum likelihood expectation maximization (MLEM) deconvolution scheme. With this very general kernel regularization term, we can stabilize the blind MLEM scheme even for the deconvolution of wide-field microscopic recordings. No complex prior point spread function models are needed. We combine state of the art optimization schemes using Tikhonov-Miller and TV regularization with our new kernel regularization. The proposed method improves the conventional deconvolution methods in terms of SNR on real and simulated datasets.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14115469" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="707eafe89aca749097b6ceafd300708f" rel="nofollow" data-download="{"attachment_id":44588801,"asset_id":14115469,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44588801/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33116184" href="https://independent.academia.edu/MajaTemerinacOtt">Maja Temerinac-Ott</a><script data-card-contents-for-user="33116184" type="text/json">{"id":33116184,"first_name":"Maja","last_name":"Temerinac-Ott","domain_name":"independent","page_name":"MajaTemerinacOtt","display_name":"Maja Temerinac-Ott","profile_url":"https://independent.academia.edu/MajaTemerinacOtt?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_14115469 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="14115469"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 14115469, container: ".js-paper-rank-work_14115469", }); 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With this very general kernel regularization term, we can stabilize the blind MLEM scheme even for the deconvolution of wide-field microscopic recordings. No complex prior point spread function models are needed. We combine state of the art optimization schemes using Tikhonov-Miller and TV regularization with our new kernel regularization. The proposed method improves the conventional deconvolution methods in terms of SNR on real and simulated datasets.","downloadable_attachments":[{"id":44588801,"asset_id":14115469,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33116184,"first_name":"Maja","last_name":"Temerinac-Ott","domain_name":"independent","page_name":"MajaTemerinacOtt","display_name":"Maja Temerinac-Ott","profile_url":"https://independent.academia.edu/MajaTemerinacOtt?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":12901,"name":"TV","url":"https://www.academia.edu/Documents/in/TV?f_ri=3091","nofollow":true},{"id":88383,"name":"Deconvolution","url":"https://www.academia.edu/Documents/in/Deconvolution?f_ri=3091","nofollow":true},{"id":96798,"name":"Noise reduction","url":"https://www.academia.edu/Documents/in/Noise_reduction?f_ri=3091","nofollow":true},{"id":191487,"name":"Kernel","url":"https://www.academia.edu/Documents/in/Kernel?f_ri=3091"},{"id":280134,"name":"Microorganisms","url":"https://www.academia.edu/Documents/in/Microorganisms?f_ri=3091"},{"id":348526,"name":"Blind Deconvolution","url":"https://www.academia.edu/Documents/in/Blind_Deconvolution?f_ri=3091"},{"id":991311,"name":"Signal to Noise Ratio","url":"https://www.academia.edu/Documents/in/Signal_to_Noise_Ratio?f_ri=3091"},{"id":1112728,"name":"Point Spread Function","url":"https://www.academia.edu/Documents/in/Point_Spread_Function?f_ri=3091"},{"id":2015985,"name":"Total Variation","url":"https://www.academia.edu/Documents/in/Total_Variation?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24210218" data-work_id="24210218" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24210218/High_resolution_SEM_imaging_of_gold_nanoparticles_in_cells_and_tissues">High resolution SEM imaging of gold nanoparticles in cells and tissues</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The growing demand of gold nanoparticles in medical applications increases the need for simple and efficient characterization methods of the interaction between the nanoparticles and biological systems. Due to its nanometre resolution,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24210218" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The growing demand of gold nanoparticles in medical applications increases the need for simple and efficient characterization methods of the interaction between the nanoparticles and biological systems. Due to its nanometre resolution, modern scanning electron microscopy (SEM) offers straightforward visualization of metallic nanoparticles down to a few nanometre size, almost without any special preparation step. However, visualization of biological materials in SEM requires complicated preparation procedure, which is typically finished by metal coating needed to decrease charging artefacts and quick radiation damage of biomaterials in the course of SEM imaging. The finest conductive metal coating available is usually composed of a few nanometre size clusters, which are almost identical to the metal nanoparticles employed in medical applications. Therefore, SEM monitoring of metal nanoparticles within cells and tissues is incompatible with the conventional preparation methods. 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itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15380690/Characterization_of_the_polycaprolactone_melt_crystallization_Complementary_optical_microscopy_DSC_and_AFM_studies">Characterization of the polycaprolactone melt crystallization: Complementary optical microscopy, DSC, and AFM studies</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">e rst stages of the crystallization of polycaprolactone (PCL) were studied using several techniques. e crystallization exotherms measured by di erential scanning calorimetry (DSC) were analyzed and compared with results obtained by... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15380690" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">e rst stages of the crystallization of polycaprolactone (PCL) were studied using several techniques. e crystallization exotherms measured by di erential scanning calorimetry (DSC) were analyzed and compared with results obtained by polarized optical microscopy (POM), rheology, and atomic force microscope (AFM). e experimental results suggest a strong in uence of the observation scale. In particular, the AFM, even if limited on time scale, appears to be the most sensitive technique to detect the rst stages of crystallization. On the contrary, at least in the case analysed in this work, rheology appears to be the least sensitive technique. DSC and POM provide closer results. is suggests that the de nition of induction time in the polymer crystallization is a vague concept that, in any case, requires the de nition of the technique used for its characterization.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15380690" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2cc4c751557abbeec620de59352bf981" rel="nofollow" data-download="{"attachment_id":43269234,"asset_id":15380690,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43269234/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34530248" href="https://cnr-it.academia.edu/AndreaSorrentino">Andrea Sorrentino</a><script data-card-contents-for-user="34530248" type="text/json">{"id":34530248,"first_name":"Andrea","last_name":"Sorrentino","domain_name":"cnr-it","page_name":"AndreaSorrentino","display_name":"Andrea Sorrentino","profile_url":"https://cnr-it.academia.edu/AndreaSorrentino?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-15380690">+1</span><div class="hidden js-additional-users-15380690"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://salerno.academia.edu/RobertoPantani">Roberto Pantani</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-15380690'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-15380690').html(); 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In particular, the AFM, even if limited on time scale, appears to be the most sensitive technique to detect the rst stages of crystallization. On the contrary, at least in the case analysed in this work, rheology appears to be the least sensitive technique. DSC and POM provide closer results. is suggests that the de nition of induction time in the polymer crystallization is a vague concept that, in any case, requires the de nition of the technique used for its characterization.","downloadable_attachments":[{"id":43269234,"asset_id":15380690,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34530248,"first_name":"Andrea","last_name":"Sorrentino","domain_name":"cnr-it","page_name":"AndreaSorrentino","display_name":"Andrea Sorrentino","profile_url":"https://cnr-it.academia.edu/AndreaSorrentino?f_ri=3091","photo":"/images/s65_no_pic.png"},{"id":4658180,"first_name":"Roberto","last_name":"Pantani","domain_name":"salerno","page_name":"RobertoPantani","display_name":"Roberto Pantani","profile_url":"https://salerno.academia.edu/RobertoPantani?f_ri=3091","photo":"https://0.academia-photos.com/4658180/2163484/2537973/s65_roberto.pantani.jpg"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":12597,"name":"Crystallization","url":"https://www.academia.edu/Documents/in/Crystallization?f_ri=3091","nofollow":true},{"id":24373,"name":"Atomic Force Microscopy","url":"https://www.academia.edu/Documents/in/Atomic_Force_Microscopy?f_ri=3091","nofollow":true},{"id":78753,"name":"Differential scanning calorimetry","url":"https://www.academia.edu/Documents/in/Differential_scanning_calorimetry?f_ri=3091","nofollow":true},{"id":973559,"name":"Polyesters","url":"https://www.academia.edu/Documents/in/Polyesters?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_17577826" data-work_id="17577826" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/17577826/Microscopy_under_pressure_An_optical_chamber_system_for_fluorescence_microscopic_analysis_of_living_cells_under_high_hydrostatic_pressure">Microscopy under pressure—An optical chamber system for fluorescence microscopic analysis of living cells under high hydrostatic pressure</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">high hydrostatic pressure microscopy; fluorescence; mammalian cell morphology ABSTRACT High hydrostatic pressure (HHP) becomes more and more interesting for life science research, since it can be employed to inactivate various cells. To... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_17577826" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">high hydrostatic pressure microscopy; fluorescence; mammalian cell morphology ABSTRACT High hydrostatic pressure (HHP) becomes more and more interesting for life science research, since it can be employed to inactivate various cells. To directly monitor ''cells under pressure,'' the development of an optical high-pressure chamber is required. Therefore, an optical pressure chamber that can be used for up to 300 MPa was constructed. This chamber has already been described as a tool for in situ observation of dynamic changes of microscopic structures in bright field as well as phase contrast. In combination with an inverted microscope, we obtained brilliant microscopic color pictures with an optical resolution more than 0.56 lm. Here, we demonstrate the capabilities of the HHP cell, in combination with epifluorescence microscopy. Using a nonadherent human B-cell line (Raji, ATCC CCL 86), stained with the fluorescent dyes propidium iodide, Hoechst 33342, or dihexyloxacarbocyanine iodide, we were able to show that the system is suitable to perform fluorescence microscopic analyses, with pressures up to 300 MPa, with viable mammalian cells. Microsc. Res. Tech. 69:65-72, 2006.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17577826" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2c9c253024d3d9858116557f5a67c128" rel="nofollow" data-download="{"attachment_id":39591840,"asset_id":17577826,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/39591840/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37397964" href="https://sysbiomed-erlangen.academia.edu/MartinHerrmann">Martin Herrmann</a><script data-card-contents-for-user="37397964" type="text/json">{"id":37397964,"first_name":"Martin","last_name":"Herrmann","domain_name":"sysbiomed-erlangen","page_name":"MartinHerrmann","display_name":"Martin Herrmann","profile_url":"https://sysbiomed-erlangen.academia.edu/MartinHerrmann?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_17577826 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="17577826"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 17577826, container: ".js-paper-rank-work_17577826", }); 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fluorescence; mammalian cell morphology ABSTRACT High hydrostatic pressure (HHP) becomes more and more interesting for life science research, since it can be employed to inactivate various cells. To directly monitor ''cells under pressure,'' the development of an optical high-pressure chamber is required. Therefore, an optical pressure chamber that can be used for up to 300 MPa was constructed. This chamber has already been described as a tool for in situ observation of dynamic changes of microscopic structures in bright field as well as phase contrast. In combination with an inverted microscope, we obtained brilliant microscopic color pictures with an optical resolution more than 0.56 lm. Here, we demonstrate the capabilities of the HHP cell, in combination with epifluorescence microscopy. Using a nonadherent human B-cell line (Raji, ATCC CCL 86), stained with the fluorescent dyes propidium iodide, Hoechst 33342, or dihexyloxacarbocyanine iodide, we were able to show that the system is suitable to perform fluorescence microscopic analyses, with pressures up to 300 MPa, with viable mammalian cells. Microsc. Res. Tech. 69:65-72, 2006.","downloadable_attachments":[{"id":39591840,"asset_id":17577826,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37397964,"first_name":"Martin","last_name":"Herrmann","domain_name":"sysbiomed-erlangen","page_name":"MartinHerrmann","display_name":"Martin Herrmann","profile_url":"https://sysbiomed-erlangen.academia.edu/MartinHerrmann?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy?f_ri=3091","nofollow":true},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=3091","nofollow":true},{"id":216976,"name":"Environmental Medicine","url":"https://www.academia.edu/Documents/in/Environmental_Medicine?f_ri=3091"},{"id":678853,"name":"B Lymphocytes","url":"https://www.academia.edu/Documents/in/B_Lymphocytes?f_ri=3091"},{"id":801413,"name":"Benzimidazoles","url":"https://www.academia.edu/Documents/in/Benzimidazoles?f_ri=3091"},{"id":1309909,"name":"Hydrostatic Pressure","url":"https://www.academia.edu/Documents/in/Hydrostatic_Pressure?f_ri=3091"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_17281252" data-work_id="17281252" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/17281252/Comparative_Study_of_the_Sensitivity_of_Different_Diagnostic_Methods_for_the_Laboratory_Diagnosis_of_Buruli_Ulcer_Disease">Comparative Study of the Sensitivity of Different Diagnostic Methods for the Laboratory Diagnosis of Buruli Ulcer Disease</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17281252" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9527efc1f21ede8a3b582f29bbb762ac" rel="nofollow" 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Sensitivity of Different Diagnostic Methods for the Laboratory Diagnosis of Buruli Ulcer Disease","created_at":"2015-10-25T11:31:49.041-07:00","url":"https://www.academia.edu/17281252/Comparative_Study_of_the_Sensitivity_of_Different_Diagnostic_Methods_for_the_Laboratory_Diagnosis_of_Buruli_Ulcer_Disease?f_ri=3091","dom_id":"work_17281252","summary":null,"downloadable_attachments":[{"id":39430082,"asset_id":17281252,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":36769444,"first_name":"Erna","last_name":"Fleischmann","domain_name":"independent","page_name":"ErnaFleischmann","display_name":"Erna Fleischmann","profile_url":"https://independent.academia.edu/ErnaFleischmann?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=3091","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=3091","nofollow":true},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child?f_ri=3091","nofollow":true},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=3091"},{"id":133057,"name":"Young Adult","url":"https://www.academia.edu/Documents/in/Young_Adult?f_ri=3091"},{"id":134346,"name":"Infant","url":"https://www.academia.edu/Documents/in/Infant?f_ri=3091"},{"id":211171,"name":"Buruli Ulcer","url":"https://www.academia.edu/Documents/in/Buruli_Ulcer?f_ri=3091"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=3091"},{"id":584601,"name":"Chi Square Distribution","url":"https://www.academia.edu/Documents/in/Chi_Square_Distribution?f_ri=3091"},{"id":801605,"name":"Mycobacterium Ulcerans","url":"https://www.academia.edu/Documents/in/Mycobacterium_Ulcerans?f_ri=3091"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=3091"},{"id":1265401,"name":"LABORATORY DIAGNOSIS","url":"https://www.academia.edu/Documents/in/LABORATORY_DIAGNOSIS?f_ri=3091"},{"id":1336838,"name":"Clinical Infectious Diseases","url":"https://www.academia.edu/Documents/in/Clinical_Infectious_Diseases?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16597498" data-work_id="16597498" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16597498/Single_molecule_microscopy_of_biomembranes_Review_">Single molecule microscopy of biomembranes (Review)</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Recent advances in the development of new microscopy techniques with a sensitivity of a single molecule have gained access to essentially new types of information obtainable from imaging biomolecular samples. These methodologies are... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16597498" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Recent advances in the development of new microscopy techniques with a sensitivity of a single molecule have gained access to essentially new types of information obtainable from imaging biomolecular samples. These methodologies are analysed here in terms of their applicability to the in vivo visualization of cellular proces ses on the molecular scale, in particular of processes in cell membranes. First examples of single molecule microscopy on cell membranes revealed new basic insight into the lateral organization of the plasma membrane, providing the captivating perspective of an ultrasensitive methodology as a general tool to study local processes and heterogeneities in living cells.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16597498" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="95f16e92b8e3eae578285176cd5cfa90" rel="nofollow" data-download="{"attachment_id":40039518,"asset_id":16597498,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40039518/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35925503" href="https://tuwien.academia.edu/GerhardSchuetz">Gerhard Schuetz</a><script data-card-contents-for-user="35925503" type="text/json">{"id":35925503,"first_name":"Gerhard","last_name":"Schuetz","domain_name":"tuwien","page_name":"GerhardSchuetz","display_name":"Gerhard Schuetz","profile_url":"https://tuwien.academia.edu/GerhardSchuetz?f_ri=3091","photo":"https://0.academia-photos.com/35925503/109887079/99129868/s65_gerhard.schuetz.png"}</script></span></span></li><li class="js-paper-rank-work_16597498 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16597498"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16597498, container: ".js-paper-rank-work_16597498", }); });</script></li><li class="js-percentile-work_16597498 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16597498; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_16597498"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16597498 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16597498"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16597498; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16597498]").text(description); $(".js-view-count-work_16597498").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16597498").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16597498"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">3</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="50157" rel="nofollow" href="https://www.academia.edu/Documents/in/Molecular">Molecular</a>, <script data-card-contents-for-ri="50157" type="text/json">{"id":50157,"name":"Molecular","url":"https://www.academia.edu/Documents/in/Molecular?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1681026" rel="nofollow" href="https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology">Biochemistry and cell biology</a><script data-card-contents-for-ri="1681026" type="text/json">{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16597498]'), work: {"id":16597498,"title":"Single molecule microscopy of biomembranes (Review)","created_at":"2015-10-09T06:11:00.293-07:00","url":"https://www.academia.edu/16597498/Single_molecule_microscopy_of_biomembranes_Review_?f_ri=3091","dom_id":"work_16597498","summary":"Recent advances in the development of new microscopy techniques with a sensitivity of a single molecule have gained access to essentially new types of information obtainable from imaging biomolecular samples. These methodologies are analysed here in terms of their applicability to the in vivo visualization of cellular proces ses on the molecular scale, in particular of processes in cell membranes. First examples of single molecule microscopy on cell membranes revealed new basic insight into the lateral organization of the plasma membrane, providing the captivating perspective of an ultrasensitive methodology as a general tool to study local processes and heterogeneities in living cells.","downloadable_attachments":[{"id":40039518,"asset_id":16597498,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35925503,"first_name":"Gerhard","last_name":"Schuetz","domain_name":"tuwien","page_name":"GerhardSchuetz","display_name":"Gerhard Schuetz","profile_url":"https://tuwien.academia.edu/GerhardSchuetz?f_ri=3091","photo":"https://0.academia-photos.com/35925503/109887079/99129868/s65_gerhard.schuetz.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":50157,"name":"Molecular","url":"https://www.academia.edu/Documents/in/Molecular?f_ri=3091","nofollow":true},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_61843649" data-work_id="61843649" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/61843649/Nassarius_kraussianus_shell_beads_from_Blombos_Cave_evidence_for_symbolic_behaviour_in_the_Middle_Stone_Age">Nassarius kraussianus shell beads from Blombos Cave: evidence for symbolic behaviour in the Middle Stone Age</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Since 1991, excavations at Blombos Cave have yielded a well-preserved sample of faunal and cultural material in Middle Stone Age (MSA) levels. The uppermost MSA phase, M1, is dated to c. 75 ka by optically stimulated luminescence (OSL)... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_61843649" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Since 1991, excavations at Blombos Cave have yielded a well-preserved sample of faunal and cultural material in Middle Stone Age (MSA) levels. The uppermost MSA phase, M1, is dated to c. 75 ka by optically stimulated luminescence (OSL) and thermoluminescence, and the middle M2 phase to a provisional c. 78 ka. Artefacts unusual in a MSA context from these phases include bifacial points, bone tools, engraved ochre and engraved bone. In this paper, we describe forty-one marine tick shell beads recovered from these MSA phases and tick shell beads from Later Stone Age (LSA) levels at Blombos Cave and the Die Kelders site. Thirty-nine shell beads come from the upper M1 phase and two from M2. Morphometric, taphonomic and microscopic analysis of modern assemblages of living and dead tick shell demonstrate that the presence of perforated Nassarius kraussianus shells in the Blombos MSA levels cannot be due to natural processes or accidental transport by humans. The types of perforation seen on the MSA shells are absent on modern accumulations of dead shells and not attributable to post-depositional damage. Their location, size, and microscopic features are similar to those obtained experimentally by piercing the shell wall, through the aperture, with a sharp bone point. Use-wear, recorded on the perforation edge, the outer lip, and the parietal wall of the aperture indicates the shells having being strung and worn. MSA shell beads differ significantly in size, perforation type, wear pattern and shade compared to LSA beads and this eliminates the possibility of mixing across respective levels.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/61843649" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="304d5e460e7848ce26ecc03ba9f05b13" rel="nofollow" data-download="{"attachment_id":74775778,"asset_id":61843649,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/74775778/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="208575308" href="https://uib.academia.edu/CHenshilwood">Christopher Henshilwood</a><script data-card-contents-for-user="208575308" type="text/json">{"id":208575308,"first_name":"Christopher","last_name":"Henshilwood","domain_name":"uib","page_name":"CHenshilwood","display_name":"Christopher Henshilwood","profile_url":"https://uib.academia.edu/CHenshilwood?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_61843649 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="61843649"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 61843649, container: ".js-paper-rank-work_61843649", }); 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$(".js-view-count[data-work-id=61843649]").text(description); $(".js-view-count-work_61843649").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_61843649").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="61843649"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="155" rel="nofollow" href="https://www.academia.edu/Documents/in/Evolutionary_Biology">Evolutionary Biology</a>, <script data-card-contents-for-ri="155" type="text/json">{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="392" rel="nofollow" href="https://www.academia.edu/Documents/in/Archaeology">Archaeology</a>, <script data-card-contents-for-ri="392" type="text/json">{"id":392,"name":"Archaeology","url":"https://www.academia.edu/Documents/in/Archaeology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="767" rel="nofollow" href="https://www.academia.edu/Documents/in/Anthropology">Anthropology</a>, <script data-card-contents-for-ri="767" type="text/json">{"id":767,"name":"Anthropology","url":"https://www.academia.edu/Documents/in/Anthropology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="772" rel="nofollow" href="https://www.academia.edu/Documents/in/Human_Evolution">Human Evolution</a><script data-card-contents-for-ri="772" type="text/json">{"id":772,"name":"Human Evolution","url":"https://www.academia.edu/Documents/in/Human_Evolution?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=61843649]'), work: {"id":61843649,"title":"Nassarius kraussianus shell beads from Blombos Cave: evidence for symbolic behaviour in the Middle Stone Age","created_at":"2021-11-17T02:47:38.955-08:00","url":"https://www.academia.edu/61843649/Nassarius_kraussianus_shell_beads_from_Blombos_Cave_evidence_for_symbolic_behaviour_in_the_Middle_Stone_Age?f_ri=3091","dom_id":"work_61843649","summary":"Since 1991, excavations at Blombos Cave have yielded a well-preserved sample of faunal and cultural material in Middle Stone Age (MSA) levels. The uppermost MSA phase, M1, is dated to c. 75 ka by optically stimulated luminescence (OSL) and thermoluminescence, and the middle M2 phase to a provisional c. 78 ka. Artefacts unusual in a MSA context from these phases include bifacial points, bone tools, engraved ochre and engraved bone. In this paper, we describe forty-one marine tick shell beads recovered from these MSA phases and tick shell beads from Later Stone Age (LSA) levels at Blombos Cave and the Die Kelders site. Thirty-nine shell beads come from the upper M1 phase and two from M2. Morphometric, taphonomic and microscopic analysis of modern assemblages of living and dead tick shell demonstrate that the presence of perforated Nassarius kraussianus shells in the Blombos MSA levels cannot be due to natural processes or accidental transport by humans. The types of perforation seen on the MSA shells are absent on modern accumulations of dead shells and not attributable to post-depositional damage. Their location, size, and microscopic features are similar to those obtained experimentally by piercing the shell wall, through the aperture, with a sharp bone point. Use-wear, recorded on the perforation edge, the outer lip, and the parietal wall of the aperture indicates the shells having being strung and worn. MSA shell beads differ significantly in size, perforation type, wear pattern and shade compared to LSA beads and this eliminates the possibility of mixing across respective levels.","downloadable_attachments":[{"id":74775778,"asset_id":61843649,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":208575308,"first_name":"Christopher","last_name":"Henshilwood","domain_name":"uib","page_name":"CHenshilwood","display_name":"Christopher Henshilwood","profile_url":"https://uib.academia.edu/CHenshilwood?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology?f_ri=3091","nofollow":true},{"id":392,"name":"Archaeology","url":"https://www.academia.edu/Documents/in/Archaeology?f_ri=3091","nofollow":true},{"id":767,"name":"Anthropology","url":"https://www.academia.edu/Documents/in/Anthropology?f_ri=3091","nofollow":true},{"id":772,"name":"Human Evolution","url":"https://www.academia.edu/Documents/in/Human_Evolution?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091"},{"id":41720,"name":"Human","url":"https://www.academia.edu/Documents/in/Human?f_ri=3091"},{"id":42758,"name":"Mesolithic","url":"https://www.academia.edu/Documents/in/Mesolithic?f_ri=3091"},{"id":48310,"name":"Cave","url":"https://www.academia.edu/Documents/in/Cave?f_ri=3091"},{"id":72667,"name":"Behaviour","url":"https://www.academia.edu/Documents/in/Behaviour?f_ri=3091"},{"id":99198,"name":"Chalcolithic","url":"https://www.academia.edu/Documents/in/Chalcolithic?f_ri=3091"},{"id":113615,"name":"Middle Stone Age","url":"https://www.academia.edu/Documents/in/Middle_Stone_Age?f_ri=3091"},{"id":155464,"name":"Excavation","url":"https://www.academia.edu/Documents/in/Excavation?f_ri=3091"},{"id":175619,"name":"Mollusca","url":"https://www.academia.edu/Documents/in/Mollusca?f_ri=3091"},{"id":413301,"name":"Perforation","url":"https://www.academia.edu/Documents/in/Perforation?f_ri=3091"},{"id":992753,"name":"Personal Ornaments In Prehistory","url":"https://www.academia.edu/Documents/in/Personal_Ornaments_In_Prehistory?f_ri=3091"},{"id":1090917,"name":"Blombos Cave","url":"https://www.academia.edu/Documents/in/Blombos_Cave?f_ri=3091"},{"id":1275015,"name":"Bead","url":"https://www.academia.edu/Documents/in/Bead?f_ri=3091"},{"id":3255947,"name":"anthropology cultural","url":"https://www.academia.edu/Documents/in/anthropology_cultural?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_75142643" data-work_id="75142643" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/75142643/Granulomatous_hepatitis_due_to_Bartonella_henselae_infection_in_an_immunocompetent_patient">Granulomatous hepatitis due to Bartonella henselae infection in an immunocompetent patient</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background: Bartonella henselae (B. henselae) is considered a rare cause of granulomatous hepatitis. Due to the fastidious growth characteristics of the bacteria, the limited sensitivity of histopathological stains, and the nonspecific... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_75142643" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: Bartonella henselae (B. henselae) is considered a rare cause of granulomatous hepatitis. Due to the fastidious growth characteristics of the bacteria, the limited sensitivity of histopathological stains, and the nonspecific histological findings on liver biopsy, the diagnosis of hepatic bartonellosis can be difficult to establish. Furthermore, the optimal treatment of established hepatic bartonellosis remains controversial. Case presentation: We present a case of hepatic bartonellosis in an immunocompetent woman who presented with right upper quadrant pain and a five cm right hepatic lobe mass on CT scan. The patient underwent a right hepatic lobectomy. Surgical pathology revealed florid necrotizing granulomatous hepatitis, favoring an infectious etiology. Despite extensive histological and serological evaluation a definitive diagnosis was not established initially. Thirteen months after initial presentation, hepatic bartonellosis was diagnosed by PCR studies from surgically excised liver tissue. Interestingly, the hepatic granulomas persisted and Bartonella henselae was isolated from the patient's enriched blood culture after several courses of antibiotic therapy. Conclusion: The diagnosis of hepatic bartonellosis is exceedingly difficult to establish and requires a high degree of clinical suspicion. Recently developed, PCR-based approaches may be required in select patients to make the diagnosis. The optimal antimicrobial therapy for hepatic bartonellosis has not been established, and close follow-up is needed to ensure successful eradication of the infection.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/75142643" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="56d0e6069ca546499180090404a873f9" rel="nofollow" data-download="{"attachment_id":83030616,"asset_id":75142643,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/83030616/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32946290" href="https://independent.academia.edu/EdwardBreitschwerdt">Edward Breitschwerdt</a><script data-card-contents-for-user="32946290" type="text/json">{"id":32946290,"first_name":"Edward","last_name":"Breitschwerdt","domain_name":"independent","page_name":"EdwardBreitschwerdt","display_name":"Edward Breitschwerdt","profile_url":"https://independent.academia.edu/EdwardBreitschwerdt?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_75142643 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="75142643"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 75142643, container: ".js-paper-rank-work_75142643", }); 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Due to the fastidious growth characteristics of the bacteria, the limited sensitivity of histopathological stains, and the nonspecific histological findings on liver biopsy, the diagnosis of hepatic bartonellosis can be difficult to establish. Furthermore, the optimal treatment of established hepatic bartonellosis remains controversial. Case presentation: We present a case of hepatic bartonellosis in an immunocompetent woman who presented with right upper quadrant pain and a five cm right hepatic lobe mass on CT scan. The patient underwent a right hepatic lobectomy. Surgical pathology revealed florid necrotizing granulomatous hepatitis, favoring an infectious etiology. Despite extensive histological and serological evaluation a definitive diagnosis was not established initially. Thirteen months after initial presentation, hepatic bartonellosis was diagnosed by PCR studies from surgically excised liver tissue. Interestingly, the hepatic granulomas persisted and Bartonella henselae was isolated from the patient's enriched blood culture after several courses of antibiotic therapy. Conclusion: The diagnosis of hepatic bartonellosis is exceedingly difficult to establish and requires a high degree of clinical suspicion. Recently developed, PCR-based approaches may be required in select patients to make the diagnosis. The optimal antimicrobial therapy for hepatic bartonellosis has not been established, and close follow-up is needed to ensure successful eradication of the infection.","downloadable_attachments":[{"id":83030616,"asset_id":75142643,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32946290,"first_name":"Edward","last_name":"Breitschwerdt","domain_name":"independent","page_name":"EdwardBreitschwerdt","display_name":"Edward Breitschwerdt","profile_url":"https://independent.academia.edu/EdwardBreitschwerdt?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=3091","nofollow":true},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=3091","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=3091"},{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=3091"},{"id":100732,"name":"Blood","url":"https://www.academia.edu/Documents/in/Blood?f_ri=3091"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=3091"},{"id":144048,"name":"Hepatitis","url":"https://www.academia.edu/Documents/in/Hepatitis?f_ri=3091"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=3091"},{"id":709514,"name":"Granuloma","url":"https://www.academia.edu/Documents/in/Granuloma?f_ri=3091"},{"id":959921,"name":"X ray Computed Tomography","url":"https://www.academia.edu/Documents/in/X_ray_Computed_Tomography?f_ri=3091"},{"id":3910440,"name":"Bartonella henselae","url":"https://www.academia.edu/Documents/in/Bartonella_henselae?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_72206054" data-work_id="72206054" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/72206054/Morphology_and_mechanical_properties_of_TPU_nanoclay_composites">Morphology and mechanical properties of TPU nanoclay composites</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Neste trabalho, compósitos de elastômero termoplástico de poliuretano (TPU) foram preparados com diferentes teores de argila (0, 1, 3 e 10% m/m). A argila Cloisite 30B (C30B) foi incorporada na matriz de TPU via processamento por fusão... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_72206054" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Neste trabalho, compósitos de elastômero termoplástico de poliuretano (TPU) foram preparados com diferentes teores de argila (0, 1, 3 e 10% m/m). A argila Cloisite 30B (C30B) foi incorporada na matriz de TPU via processamento por fusão (extrusora de dupla rosca; compósitos TPU-E) e polimerização em massa (compósitos TPU-S). O método de síntese foi a polimerização em duas etapas do poliol poliéster (massa molar de 2000 g mol-1) e do di-isocianato de 4,4' difenil metano (MDI) com 1,4-butanodiol como extensor de cadeia. A dispersão da argila e seu efeito sobre as propriedades mecânicas dos compósitos foram investigadas. A caracterização dos compósitos de TPU com argila foi realizada através da microscopia eletrônica de varredura (MEV) e da difração de raios-X (DRX). As propriedades mecânicas foram avaliadas através da resistência à tração e ao rasgamento. O TPU-E com 3% de argila apresentou os melhores resultados com aumento nos valores de tensão e no alongamento na ruptura (28% e 35%, respectivamente) e na energia (88%), comparado ao TPU-E (amostra sem argila). Palavras-chave: TPU, argila, microscopia eletrônica de varredura, difração de raios-X, propriedades mecânicas. Morphology and Mechanical Properties of TPU Nanoclay Composites In this study thermoplastic polyurethane (TPU) composites were obtained with different nanoclay contents (0, 1, 3 for all cases and 10 wt% in some cases). The nanoclay Cloisite 30B (C30B) was dispersed in the TPU matrix by melt processing (twin-screw extruder; TPU-E composites) and during bulk polymerization (TPU-S composites). The synthesis method involved the two-step bulk polymerization of polyesterpolyol (molecular weight 2.000 g mol-1) and diphenylmethanediisocyanate (MDI) with 1,4-butanediol as the chain extender. The dispersion state of the nanoclay particles and its effect on the mechanical properties of the composites was investigated. The characterization of TPU/nanoclay composites was carried out by means of scanning electron microscopy (SEM) and X-ray diffraction (XRD). The mechanical characterization was carried out by means of tensile and tear strengths test. The TPU-E 3 wt% composite showed the best improvement with increases in stress and strain at break (28% and 35%, respectively) and energy (88%), compared to the TPU-E (sample without nanoclay).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/72206054" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c5b79370a560950427f07f6cc2f0a2b0" rel="nofollow" data-download="{"attachment_id":81226367,"asset_id":72206054,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/81226367/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32744593" href="https://independent.academia.edu/AdemirZattera">Ademir Zattera</a><script data-card-contents-for-user="32744593" type="text/json">{"id":32744593,"first_name":"Ademir","last_name":"Zattera","domain_name":"independent","page_name":"AdemirZattera","display_name":"Ademir Zattera","profile_url":"https://independent.academia.edu/AdemirZattera?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_72206054 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="72206054"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 72206054, container: ".js-paper-rank-work_72206054", }); 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$(".js-view-count[data-work-id=72206054]").text(description); $(".js-view-count-work_72206054").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_72206054").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="72206054"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="511" rel="nofollow" href="https://www.academia.edu/Documents/in/Materials_Science">Materials Science</a>, <script data-card-contents-for-ri="511" type="text/json">{"id":511,"name":"Materials Science","url":"https://www.academia.edu/Documents/in/Materials_Science?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1177" rel="nofollow" href="https://www.academia.edu/Documents/in/Crystallography">Crystallography</a>, <script data-card-contents-for-ri="1177" type="text/json">{"id":1177,"name":"Crystallography","url":"https://www.academia.edu/Documents/in/Crystallography?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2184" rel="nofollow" href="https://www.academia.edu/Documents/in/Electron_Microscopy">Electron Microscopy</a>, <script data-card-contents-for-ri="2184" type="text/json">{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a><script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=72206054]'), work: {"id":72206054,"title":"Morphology and mechanical properties of TPU nanoclay composites","created_at":"2022-02-22T02:55:50.232-08:00","url":"https://www.academia.edu/72206054/Morphology_and_mechanical_properties_of_TPU_nanoclay_composites?f_ri=3091","dom_id":"work_72206054","summary":"Neste trabalho, compósitos de elastômero termoplástico de poliuretano (TPU) foram preparados com diferentes teores de argila (0, 1, 3 e 10% m/m). A argila Cloisite 30B (C30B) foi incorporada na matriz de TPU via processamento por fusão (extrusora de dupla rosca; compósitos TPU-E) e polimerização em massa (compósitos TPU-S). O método de síntese foi a polimerização em duas etapas do poliol poliéster (massa molar de 2000 g mol-1) e do di-isocianato de 4,4' difenil metano (MDI) com 1,4-butanodiol como extensor de cadeia. A dispersão da argila e seu efeito sobre as propriedades mecânicas dos compósitos foram investigadas. A caracterização dos compósitos de TPU com argila foi realizada através da microscopia eletrônica de varredura (MEV) e da difração de raios-X (DRX). As propriedades mecânicas foram avaliadas através da resistência à tração e ao rasgamento. O TPU-E com 3% de argila apresentou os melhores resultados com aumento nos valores de tensão e no alongamento na ruptura (28% e 35%, respectivamente) e na energia (88%), comparado ao TPU-E (amostra sem argila). Palavras-chave: TPU, argila, microscopia eletrônica de varredura, difração de raios-X, propriedades mecânicas. Morphology and Mechanical Properties of TPU Nanoclay Composites In this study thermoplastic polyurethane (TPU) composites were obtained with different nanoclay contents (0, 1, 3 for all cases and 10 wt% in some cases). The nanoclay Cloisite 30B (C30B) was dispersed in the TPU matrix by melt processing (twin-screw extruder; TPU-E composites) and during bulk polymerization (TPU-S composites). The synthesis method involved the two-step bulk polymerization of polyesterpolyol (molecular weight 2.000 g mol-1) and diphenylmethanediisocyanate (MDI) with 1,4-butanediol as the chain extender. The dispersion state of the nanoclay particles and its effect on the mechanical properties of the composites was investigated. The characterization of TPU/nanoclay composites was carried out by means of scanning electron microscopy (SEM) and X-ray diffraction (XRD). The mechanical characterization was carried out by means of tensile and tear strengths test. The TPU-E 3 wt% composite showed the best improvement with increases in stress and strain at break (28% and 35%, respectively) and energy (88%), compared to the TPU-E (sample without nanoclay).","downloadable_attachments":[{"id":81226367,"asset_id":72206054,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32744593,"first_name":"Ademir","last_name":"Zattera","domain_name":"independent","page_name":"AdemirZattera","display_name":"Ademir Zattera","profile_url":"https://independent.academia.edu/AdemirZattera?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":511,"name":"Materials Science","url":"https://www.academia.edu/Documents/in/Materials_Science?f_ri=3091","nofollow":true},{"id":1177,"name":"Crystallography","url":"https://www.academia.edu/Documents/in/Crystallography?f_ri=3091","nofollow":true},{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":6309,"name":"Metallurgy","url":"https://www.academia.edu/Documents/in/Metallurgy?f_ri=3091"},{"id":10655,"name":"Scanning Electron Microscopy","url":"https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy?f_ri=3091"},{"id":32909,"name":"Polymerization","url":"https://www.academia.edu/Documents/in/Polymerization?f_ri=3091"},{"id":73142,"name":"Electromagnetic Radiation","url":"https://www.academia.edu/Documents/in/Electromagnetic_Radiation?f_ri=3091"},{"id":104708,"name":"Plastics","url":"https://www.academia.edu/Documents/in/Plastics?f_ri=3091"},{"id":165106,"name":"Diffraction","url":"https://www.academia.edu/Documents/in/Diffraction?f_ri=3091"},{"id":213373,"name":"Petrochemicals","url":"https://www.academia.edu/Documents/in/Petrochemicals?f_ri=3091"},{"id":255044,"name":"Chemical Reactions","url":"https://www.academia.edu/Documents/in/Chemical_Reactions?f_ri=3091"},{"id":334693,"name":"Polyamides","url":"https://www.academia.edu/Documents/in/Polyamides?f_ri=3091"},{"id":386527,"name":"X ray diffraction","url":"https://www.academia.edu/Documents/in/X_ray_diffraction?f_ri=3091"},{"id":441926,"name":"Scattering","url":"https://www.academia.edu/Documents/in/Scattering?f_ri=3091"},{"id":764266,"name":"Ionizing radiations","url":"https://www.academia.edu/Documents/in/Ionizing_radiations?f_ri=3091"},{"id":1146710,"name":"Curing","url":"https://www.academia.edu/Documents/in/Curing?f_ri=3091"},{"id":2236565,"name":"Petroleum Products","url":"https://www.academia.edu/Documents/in/Petroleum_Products?f_ri=3091"},{"id":2417735,"name":"Synthetic Materials","url":"https://www.academia.edu/Documents/in/Synthetic_Materials?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_61312560" data-work_id="61312560" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/61312560/Primary_non_Hodgkins_lymphoma_of_the_salivary_gland_A_spectrum_of_lymphoepithelial_sialadenitis_low_grade_B_cell_lymphoma_of_mucosa_associated_lymphoid_tissue_">Primary non-Hodgkin's lymphoma of the salivary gland: A spectrum of lymphoepithelial sialadenitis, low-grade B-cell lymphoma of mucosa-associated lymphoid tissue …</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Lymphoid infi ltrates of the salivary gland can be either reactive or neoplastic. The reactive lesion, lymphoepithelial sialadenitis (LESA) may be associated with Sjogren's syndrome (SS) or may occur as an isolated salivary gland... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_61312560" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Lymphoid infi ltrates of the salivary gland can be either reactive or neoplastic. The reactive lesion, lymphoepithelial sialadenitis (LESA) may be associated with Sjogren's syndrome (SS) or may occur as an isolated salivary gland enlargement. Patients with LESA/SS have a particularly high risk of subsequently developing lymphoma, which is a low-grade mucosa-associated lymphoid tissue (MALT) type lymphoma of the salivary gland. We document a rare case of primary non-Hodgkin's lymphoma of the parotid gland arising in the background of LESA and with a rare example of transformation from low grade to high-grade B cell lymphoma of MALT type.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/61312560" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e6eca7b3c8cac7de5a0c54c237c98a8e" rel="nofollow" data-download="{"attachment_id":74378209,"asset_id":61312560,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/74378209/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33285643" href="https://independent.academia.edu/MeenalHastak">Meenal Hastak</a><script data-card-contents-for-user="33285643" type="text/json">{"id":33285643,"first_name":"Meenal","last_name":"Hastak","domain_name":"independent","page_name":"MeenalHastak","display_name":"Meenal Hastak","profile_url":"https://independent.academia.edu/MeenalHastak?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_61312560 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="61312560"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 61312560, container: ".js-paper-rank-work_61312560", }); });</script></li><li class="js-percentile-work_61312560 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 61312560; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_61312560"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_61312560 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="61312560"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 61312560; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=61312560]").text(description); $(".js-view-count-work_61312560").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_61312560").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="61312560"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" rel="nofollow" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="321836" rel="nofollow" href="https://www.academia.edu/Documents/in/Spectrum">Spectrum</a><script data-card-contents-for-ri="321836" type="text/json">{"id":321836,"name":"Spectrum","url":"https://www.academia.edu/Documents/in/Spectrum?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=61312560]'), work: {"id":61312560,"title":"Primary non-Hodgkin's lymphoma of the salivary gland: A spectrum of lymphoepithelial sialadenitis, low-grade B-cell lymphoma of mucosa-associated lymphoid tissue …","created_at":"2021-11-08T05:56:14.620-08:00","url":"https://www.academia.edu/61312560/Primary_non_Hodgkins_lymphoma_of_the_salivary_gland_A_spectrum_of_lymphoepithelial_sialadenitis_low_grade_B_cell_lymphoma_of_mucosa_associated_lymphoid_tissue_?f_ri=3091","dom_id":"work_61312560","summary":"Lymphoid infi ltrates of the salivary gland can be either reactive or neoplastic. The reactive lesion, lymphoepithelial sialadenitis (LESA) may be associated with Sjogren's syndrome (SS) or may occur as an isolated salivary gland enlargement. Patients with LESA/SS have a particularly high risk of subsequently developing lymphoma, which is a low-grade mucosa-associated lymphoid tissue (MALT) type lymphoma of the salivary gland. We document a rare case of primary non-Hodgkin's lymphoma of the parotid gland arising in the background of LESA and with a rare example of transformation from low grade to high-grade B cell lymphoma of MALT type.","downloadable_attachments":[{"id":74378209,"asset_id":61312560,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33285643,"first_name":"Meenal","last_name":"Hastak","domain_name":"independent","page_name":"MeenalHastak","display_name":"Meenal Hastak","profile_url":"https://independent.academia.edu/MeenalHastak?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=3091","nofollow":true},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=3091","nofollow":true},{"id":321836,"name":"Spectrum","url":"https://www.academia.edu/Documents/in/Spectrum?f_ri=3091","nofollow":true},{"id":541328,"name":"Diffuse Large B-Cell Lymphoma","url":"https://www.academia.edu/Documents/in/Diffuse_Large_B-Cell_Lymphoma?f_ri=3091"},{"id":792057,"name":"Parotid Gland","url":"https://www.academia.edu/Documents/in/Parotid_Gland?f_ri=3091"},{"id":1425658,"name":"Lymphoid Tissue","url":"https://www.academia.edu/Documents/in/Lymphoid_Tissue?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_59872222" data-work_id="59872222" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/59872222/Label_free_haemogram_using_wavelength_modulated_Raman_spectroscopy_for_identifying_immune_cell_subset">Label-free haemogram using wavelength modulated Raman spectroscopy for identifying immune-cell subset</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Leucocytes in the blood of mammals form a powerful protective system against a wide range of dangerous pathogens. There are several types of immune cells that has specific role in the whole immune system. The number and type of immune... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_59872222" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Leucocytes in the blood of mammals form a powerful protective system against a wide range of dangerous pathogens. There are several types of immune cells that has specific role in the whole immune system. The number and type of immune cells alter in the disease state and identifying the type of immune cell provides information about a person's state of health. There are several immune cell subsets that are essentially morphologically identical and require external labeling to enable discrimination. Here we demonstrate the feasibility of using Wavelength Modulated Raman Spectroscopy (WMRS) with suitable machine learning algorithms as a label-free method to distinguish between different closely lying immune cell subset. Principal Component Analysis (PCA) was performed on WMRS data from single cells, obtained using confocal Raman microscopy for feature reduction, followed by Support Vector Machine (SVM) for binary discrimination of various cell subset, which yielded an accuracy >85%. The method was successful in discriminating between untouched and unfixed purified populations of CD4+CD3+ and CD8+CD3+ T lymphocyte subsets, and CD56+CD3-natural killer cells with a high degree of specificity. It was also proved sensitive enough to identify unique Raman signatures that allow clear discrimination between dendritic cell subsets, comprising CD303+CD45+ plasmacytoid and CD1c+CD141+ myeloid dendritic cells. The results of this study clearly show that WMRS is highly sensitive and can distinguish between cell types that are morphologically identical.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/59872222" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="35d00392ed0e3c01ac742abbf178eb93" rel="nofollow" data-download="{"attachment_id":73572088,"asset_id":59872222,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/73572088/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="150312" href="https://st-andrews.academia.edu/PraveenAshok">Praveen Ashok</a><script data-card-contents-for-user="150312" type="text/json">{"id":150312,"first_name":"Praveen","last_name":"Ashok","domain_name":"st-andrews","page_name":"PraveenAshok","display_name":"Praveen Ashok","profile_url":"https://st-andrews.academia.edu/PraveenAshok?f_ri=3091","photo":"https://0.academia-photos.com/150312/39639/36418/s65_praveen.ashok.jpeg"}</script></span></span></li><li class="js-paper-rank-work_59872222 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="59872222"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 59872222, container: ".js-paper-rank-work_59872222", }); });</script></li><li class="js-percentile-work_59872222 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 59872222; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_59872222"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_59872222 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="59872222"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 59872222; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=59872222]").text(description); $(".js-view-count-work_59872222").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_59872222").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="59872222"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="428" rel="nofollow" href="https://www.academia.edu/Documents/in/Algorithms">Algorithms</a>, <script data-card-contents-for-ri="428" type="text/json">{"id":428,"name":"Algorithms","url":"https://www.academia.edu/Documents/in/Algorithms?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2008" rel="nofollow" href="https://www.academia.edu/Documents/in/Machine_Learning">Machine Learning</a>, <script data-card-contents-for-ri="2008" type="text/json">{"id":2008,"name":"Machine Learning","url":"https://www.academia.edu/Documents/in/Machine_Learning?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5069" rel="nofollow" href="https://www.academia.edu/Documents/in/Principal_Component_Analysis">Principal Component Analysis</a><script data-card-contents-for-ri="5069" type="text/json">{"id":5069,"name":"Principal Component Analysis","url":"https://www.academia.edu/Documents/in/Principal_Component_Analysis?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=59872222]'), work: {"id":59872222,"title":"Label-free haemogram using wavelength modulated Raman spectroscopy for identifying immune-cell subset","created_at":"2021-10-24T22:52:31.078-07:00","url":"https://www.academia.edu/59872222/Label_free_haemogram_using_wavelength_modulated_Raman_spectroscopy_for_identifying_immune_cell_subset?f_ri=3091","dom_id":"work_59872222","summary":"Leucocytes in the blood of mammals form a powerful protective system against a wide range of dangerous pathogens. There are several types of immune cells that has specific role in the whole immune system. The number and type of immune cells alter in the disease state and identifying the type of immune cell provides information about a person's state of health. There are several immune cell subsets that are essentially morphologically identical and require external labeling to enable discrimination. Here we demonstrate the feasibility of using Wavelength Modulated Raman Spectroscopy (WMRS) with suitable machine learning algorithms as a label-free method to distinguish between different closely lying immune cell subset. Principal Component Analysis (PCA) was performed on WMRS data from single cells, obtained using confocal Raman microscopy for feature reduction, followed by Support Vector Machine (SVM) for binary discrimination of various cell subset, which yielded an accuracy \u003e85%. The method was successful in discriminating between untouched and unfixed purified populations of CD4+CD3+ and CD8+CD3+ T lymphocyte subsets, and CD56+CD3-natural killer cells with a high degree of specificity. It was also proved sensitive enough to identify unique Raman signatures that allow clear discrimination between dendritic cell subsets, comprising CD303+CD45+ plasmacytoid and CD1c+CD141+ myeloid dendritic cells. The results of this study clearly show that WMRS is highly sensitive and can distinguish between cell types that are morphologically identical.","downloadable_attachments":[{"id":73572088,"asset_id":59872222,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":150312,"first_name":"Praveen","last_name":"Ashok","domain_name":"st-andrews","page_name":"PraveenAshok","display_name":"Praveen Ashok","profile_url":"https://st-andrews.academia.edu/PraveenAshok?f_ri=3091","photo":"https://0.academia-photos.com/150312/39639/36418/s65_praveen.ashok.jpeg"}],"research_interests":[{"id":428,"name":"Algorithms","url":"https://www.academia.edu/Documents/in/Algorithms?f_ri=3091","nofollow":true},{"id":2008,"name":"Machine Learning","url":"https://www.academia.edu/Documents/in/Machine_Learning?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":5069,"name":"Principal Component Analysis","url":"https://www.academia.edu/Documents/in/Principal_Component_Analysis?f_ri=3091","nofollow":true},{"id":9339,"name":"Raman Spectroscopy","url":"https://www.academia.edu/Documents/in/Raman_Spectroscopy?f_ri=3091"},{"id":43619,"name":"Feature Selection","url":"https://www.academia.edu/Documents/in/Feature_Selection?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_50127974" data-work_id="50127974" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/50127974/Expression_of_villin_in_the_mouse_oviduct_and_the_seminiferous_ducts">Expression of villin in the mouse oviduct and the seminiferous ducts</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Villin, a 95-kD cytoskeletal protein selectively expressed in the microvilli of some absorptive cells was localized immunohistochemically in the oviduct and the seminiferous excretory ducts of the mouse. Villin was found in the proximal... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_50127974" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Villin, a 95-kD cytoskeletal protein selectively expressed in the microvilli of some absorptive cells was localized immunohistochemically in the oviduct and the seminiferous excretory ducts of the mouse. Villin was found in the proximal part of the oviduct, comprising the preampulla, ampulla, and part of the isthmus. Distal to the isthmus the oviductal cells lining the junctura and the intrauterine colliculus tubaris were devoid of villin. No villin could be detected in the uterine cells. Ductuli efferentes, connecting the rete testis with the epididymis were the only portion of the male seminiferous ductal system expressing villin. The cells lining the epididymis and the vas deferens were devoid of villin. These data show that villin is selectively expressed in male and female reproductive systems and that it is limited to anatomically defined proximal portions of the reproductive ducts.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/50127974" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="511663e3fd7e678b56fecbc740347f84" rel="nofollow" data-download="{"attachment_id":68229972,"asset_id":50127974,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/68229972/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="1106127" href="https://kumc.academia.edu/IvanDamjanov">Ivan Damjanov</a><script data-card-contents-for-user="1106127" type="text/json">{"id":1106127,"first_name":"Ivan","last_name":"Damjanov","domain_name":"kumc","page_name":"IvanDamjanov","display_name":"Ivan Damjanov","profile_url":"https://kumc.academia.edu/IvanDamjanov?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_50127974 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="50127974"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 50127974, container: ".js-paper-rank-work_50127974", }); });</script></li><li class="js-percentile-work_50127974 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50127974; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_50127974"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_50127974 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="50127974"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50127974; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50127974]").text(description); $(".js-view-count-work_50127974").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_50127974").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="50127974"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="10194" rel="nofollow" href="https://www.academia.edu/Documents/in/Localization">Localization</a>, <script data-card-contents-for-ri="10194" type="text/json">{"id":10194,"name":"Localization","url":"https://www.academia.edu/Documents/in/Localization?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12070" rel="nofollow" href="https://www.academia.edu/Documents/in/Histochemistry">Histochemistry</a>, <script data-card-contents-for-ri="12070" type="text/json">{"id":12070,"name":"Histochemistry","url":"https://www.academia.edu/Documents/in/Histochemistry?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a><script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=50127974]'), work: {"id":50127974,"title":"Expression of villin in the mouse oviduct and the seminiferous ducts","created_at":"2021-07-21T05:49:02.147-07:00","url":"https://www.academia.edu/50127974/Expression_of_villin_in_the_mouse_oviduct_and_the_seminiferous_ducts?f_ri=3091","dom_id":"work_50127974","summary":"Villin, a 95-kD cytoskeletal protein selectively expressed in the microvilli of some absorptive cells was localized immunohistochemically in the oviduct and the seminiferous excretory ducts of the mouse. Villin was found in the proximal part of the oviduct, comprising the preampulla, ampulla, and part of the isthmus. Distal to the isthmus the oviductal cells lining the junctura and the intrauterine colliculus tubaris were devoid of villin. No villin could be detected in the uterine cells. Ductuli efferentes, connecting the rete testis with the epididymis were the only portion of the male seminiferous ductal system expressing villin. The cells lining the epididymis and the vas deferens were devoid of villin. These data show that villin is selectively expressed in male and female reproductive systems and that it is limited to anatomically defined proximal portions of the reproductive ducts.","downloadable_attachments":[{"id":68229972,"asset_id":50127974,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":1106127,"first_name":"Ivan","last_name":"Damjanov","domain_name":"kumc","page_name":"IvanDamjanov","display_name":"Ivan Damjanov","profile_url":"https://kumc.academia.edu/IvanDamjanov?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":10194,"name":"Localization","url":"https://www.academia.edu/Documents/in/Localization?f_ri=3091","nofollow":true},{"id":12070,"name":"Histochemistry","url":"https://www.academia.edu/Documents/in/Histochemistry?f_ri=3091","nofollow":true},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=3091","nofollow":true},{"id":37508,"name":"Cytoskeleton","url":"https://www.academia.edu/Documents/in/Cytoskeleton?f_ri=3091"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=3091"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=3091"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins?f_ri=3091"},{"id":203521,"name":"tESTIS","url":"https://www.academia.edu/Documents/in/tESTIS?f_ri=3091"},{"id":1944525,"name":"Seminiferous tubules","url":"https://www.academia.edu/Documents/in/Seminiferous_tubules?f_ri=3091"},{"id":3194201,"name":"Fallopian tubes","url":"https://www.academia.edu/Documents/in/Fallopian_tubes?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_45236149" data-work_id="45236149" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/45236149/Flat_embedding_and_immunolabelling_of_SW_1116_colon_carcinoma_cells_in_LR_white_An_improved_technique_in_light_and_electron_microscopy">Flat embedding and immunolabelling of SW 1116 colon carcinoma cells in LR white: An improved technique in light and electron microscopy</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Human SW 1116 colon carcinoma cells were grown on matrix-covered coverslips and flat embedded in specially prepared gelatin capsules in the hydrophylic resin LR White. Dehydration and polymerization were carried out so as to maximize... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_45236149" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Human SW 1116 colon carcinoma cells were grown on matrix-covered coverslips and flat embedded in specially prepared gelatin capsules in the hydrophylic resin LR White. Dehydration and polymerization were carried out so as to maximize preservation of antigenicity. Sections were cut perpendicular to the substratum. To visualize mucin, semithin sections of SW 1116 cells were stained with periodic acid Schiff (PAS) reagent for light microscopy, and ultrathin sections were labelled with a monoclonal mucin antibody (Mab 19-9) and immunogold for electron microscopy. Immunofluorescence was carried out on whole cultured cells using Mab 19-9. The morphological preservation of SW 1116 cells embedded in LR White was comparable to that of Epon-embedded cells. Mucin was localized on the microvillar surface of the apical plasma membrane and occasionally in intercellular spaces between adjacent cells. Mucin was also present in vesicles in the apical and lateral part, and to a lesser extent in the basal part of the cells. We conclude that this new technology significantly improves the morphological preservation of cells and tissues in LR White, while also serving to sustain the antigenicity of cellular antigens.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/45236149" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="869ca41c035702664947700530e0b117" rel="nofollow" data-download="{"attachment_id":65808954,"asset_id":45236149,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/65808954/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33654583" href="https://independent.academia.edu/HarveyPollard1">Harvey Pollard</a><script data-card-contents-for-user="33654583" type="text/json">{"id":33654583,"first_name":"Harvey","last_name":"Pollard","domain_name":"independent","page_name":"HarveyPollard1","display_name":"Harvey Pollard","profile_url":"https://independent.academia.edu/HarveyPollard1?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_45236149 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="45236149"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 45236149, container: ".js-paper-rank-work_45236149", }); 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Dehydration and polymerization were carried out so as to maximize preservation of antigenicity. Sections were cut perpendicular to the substratum. To visualize mucin, semithin sections of SW 1116 cells were stained with periodic acid Schiff (PAS) reagent for light microscopy, and ultrathin sections were labelled with a monoclonal mucin antibody (Mab 19-9) and immunogold for electron microscopy. Immunofluorescence was carried out on whole cultured cells using Mab 19-9. The morphological preservation of SW 1116 cells embedded in LR White was comparable to that of Epon-embedded cells. Mucin was localized on the microvillar surface of the apical plasma membrane and occasionally in intercellular spaces between adjacent cells. Mucin was also present in vesicles in the apical and lateral part, and to a lesser extent in the basal part of the cells. We conclude that this new technology significantly improves the morphological preservation of cells and tissues in LR White, while also serving to sustain the antigenicity of cellular antigens.","downloadable_attachments":[{"id":65808954,"asset_id":45236149,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33654583,"first_name":"Harvey","last_name":"Pollard","domain_name":"independent","page_name":"HarveyPollard1","display_name":"Harvey Pollard","profile_url":"https://independent.academia.edu/HarveyPollard1?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers?f_ri=3091","nofollow":true},{"id":118365,"name":"Mucin","url":"https://www.academia.edu/Documents/in/Mucin?f_ri=3091"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=3091"},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique?f_ri=3091"},{"id":604754,"name":"Acrylic Resins","url":"https://www.academia.edu/Documents/in/Acrylic_Resins?f_ri=3091"},{"id":936268,"name":"Colon Carcinoma","url":"https://www.academia.edu/Documents/in/Colon_Carcinoma?f_ri=3091"},{"id":1036479,"name":"Fixatives","url":"https://www.academia.edu/Documents/in/Fixatives?f_ri=3091"},{"id":1257712,"name":"Mucins","url":"https://www.academia.edu/Documents/in/Mucins?f_ri=3091"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091"},{"id":3793278,"name":"Colonic Neoplasms","url":"https://www.academia.edu/Documents/in/Colonic_Neoplasms?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3117151" data-work_id="3117151" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3117151/Topics_in_Electron_Diffraction_and_Microscopy_of_Materials_Edited_by_P_B_Hirsch_Part_of_the_Microscopy_in_Materials_Science_series_Institute_of_Physics_Press_1999_Hardback_196_pp_f75_120_ISBN_07503_0538_X">Topics in Electron Diffraction and Microscopy of Materials. Edited by P. B. Hirsch. Part of the Microscopy in Materials Science series. Institute of Physics Press, 1999. Hardback, 196 pp f75, $120. ISBN 07503 0538 X</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3117151" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="20152c13e7cbb9eb1807fac1de0f3bdd" rel="nofollow" data-download="{"attachment_id":50453595,"asset_id":3117151,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50453595/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="100762" href="https://liverpool.academia.edu/PeterGoodhew">Peter Goodhew</a><script data-card-contents-for-user="100762" type="text/json">{"id":100762,"first_name":"Peter","last_name":"Goodhew","domain_name":"liverpool","page_name":"PeterGoodhew","display_name":"Peter Goodhew","profile_url":"https://liverpool.academia.edu/PeterGoodhew?f_ri=3091","photo":"https://0.academia-photos.com/100762/27758/25558/s65_peter.goodhew.jpeg"}</script></span></span></li><li class="js-paper-rank-work_3117151 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="3117151"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 3117151, container: ".js-paper-rank-work_3117151", }); 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$(".js-view-count[data-work-id=3117151]").text(description); $(".js-view-count-work_3117151").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_3117151").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="3117151"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">4</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="56" rel="nofollow" href="https://www.academia.edu/Documents/in/Materials_Engineering">Materials Engineering</a>, <script data-card-contents-for-ri="56" type="text/json">{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="505" rel="nofollow" href="https://www.academia.edu/Documents/in/Condensed_Matter_Physics">Condensed Matter Physics</a>, <script data-card-contents-for-ri="505" type="text/json">{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" rel="nofollow" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1681026" rel="nofollow" href="https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology">Biochemistry and cell biology</a><script data-card-contents-for-ri="1681026" type="text/json">{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=3117151]'), work: {"id":3117151,"title":"Topics in Electron Diffraction and Microscopy of Materials. Edited by P. B. Hirsch. Part of the Microscopy in Materials Science series. Institute of Physics Press, 1999. Hardback, 196 pp f75, $120. ISBN 07503 0538 X","created_at":"2013-03-26T06:57:24.822-07:00","url":"https://www.academia.edu/3117151/Topics_in_Electron_Diffraction_and_Microscopy_of_Materials_Edited_by_P_B_Hirsch_Part_of_the_Microscopy_in_Materials_Science_series_Institute_of_Physics_Press_1999_Hardback_196_pp_f75_120_ISBN_07503_0538_X?f_ri=3091","dom_id":"work_3117151","summary":null,"downloadable_attachments":[{"id":50453595,"asset_id":3117151,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":100762,"first_name":"Peter","last_name":"Goodhew","domain_name":"liverpool","page_name":"PeterGoodhew","display_name":"Peter Goodhew","profile_url":"https://liverpool.academia.edu/PeterGoodhew?f_ri=3091","photo":"https://0.academia-photos.com/100762/27758/25558/s65_peter.goodhew.jpeg"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_4281612" data-work_id="4281612" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/4281612/Ni_Cu_Zn_Ferrite_Prepared_by_Aloe_Vera_Plant_Extract_or_Egg_White">Ni-Cu-Zn Ferrite Prepared by Aloe Vera Plant Extract or Egg White</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Ferrites (AFe 2 O 4 , A = Mn, Co, Ni, Cu, Zn), particularly nanoparticles, have high potential for many new technological applications, e.g. magnetic recording media and storage, MRI enhancement, magnetically guided drug delivery, and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_4281612" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Ferrites (AFe 2 O 4 , A = Mn, Co, Ni, Cu, Zn), particularly nanoparticles, have high potential for many new technological applications, e.g. magnetic recording media and storage, MRI enhancement, magnetically guided drug delivery, and waste water treatment. Spinel zinc ferrite (ZnFe 2 O 4 ) is paramagnetic and exhibits antiferromagnetism with the Neel temperature (T N ) of about 10K [1]. On the other hand, nickel ferrite (Ni Fe 2 O 4 ) has an inverse spinel structure showing ferri-magnetism that originates from magnetic moment of anti-parrallel spins between Fe 3+ ions at tetrahedral sites and Ni 2+ ions at octahedral sites . It was suggested recently that some Zn, Ni and Cu ferrites offer superior magnetic properties depending on certain concentration of Ni, Cu, Zn in the ferrites . The present study reports on the effects of Zn, Ni, and Cu concentrations on the structural and magnetic properties of the ferrites prepared by simple, cost-effective, non-toxic sol-gel methods.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4281612" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="64e454f27ad15b443d7ceac64089a3c6" rel="nofollow" data-download="{"attachment_id":49961611,"asset_id":4281612,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49961611/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="5198385" href="https://independent.academia.edu/DUONGBINH">BINH DUONG</a><script data-card-contents-for-user="5198385" type="text/json">{"id":5198385,"first_name":"BINH","last_name":"DUONG","domain_name":"independent","page_name":"DUONGBINH","display_name":"BINH DUONG","profile_url":"https://independent.academia.edu/DUONGBINH?f_ri=3091","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_4281612 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="4281612"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 4281612, container: ".js-paper-rank-work_4281612", }); 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Spinel zinc ferrite (ZnFe 2 O 4 ) is paramagnetic and exhibits antiferromagnetism with the Neel temperature (T N ) of about 10K [1]. On the other hand, nickel ferrite (Ni Fe 2 O 4 ) has an inverse spinel structure showing ferri-magnetism that originates from magnetic moment of anti-parrallel spins between Fe 3+ ions at tetrahedral sites and Ni 2+ ions at octahedral sites . It was suggested recently that some Zn, Ni and Cu ferrites offer superior magnetic properties depending on certain concentration of Ni, Cu, Zn in the ferrites . The present study reports on the effects of Zn, Ni, and Cu concentrations on the structural and magnetic properties of the ferrites prepared by simple, cost-effective, non-toxic sol-gel methods.","downloadable_attachments":[{"id":49961611,"asset_id":4281612,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":5198385,"first_name":"BINH","last_name":"DUONG","domain_name":"independent","page_name":"DUONGBINH","display_name":"BINH DUONG","profile_url":"https://independent.academia.edu/DUONGBINH?f_ri=3091","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=3091","nofollow":true},{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":732398,"name":"Plant Extract","url":"https://www.academia.edu/Documents/in/Plant_Extract?f_ri=3091","nofollow":true},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=3091"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_4529814" data-work_id="4529814" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/4529814/A_color_object_recognition_scheme_application_to_cellular_sorting">A color object recognition scheme: application to cellular sorting</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This paper presents a color object recognition scheme which proceeds in three sequential steps: segmentation, features extraction and classification. We mainly focus on the first and the third steps here. A color watershed using global... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_4529814" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This paper presents a color object recognition scheme which proceeds in three sequential steps: segmentation, features extraction and classification. We mainly focus on the first and the third steps here. A color watershed using global and local criteria is first described. A color contrast value is defined to select the best color space for segmenting color objects. Then, an architecture of binary neural networks is described. Its properties relies on the simplification of the recognition problem, leading to a noticeable increase in the classification rate. We conclude with the abilities of such a recognition scheme and present an automated cell sorting system.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4529814" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e90956cec3f4304e8e09a66d0d53b0dc" rel="nofollow" data-download="{"attachment_id":49803417,"asset_id":4529814,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49803417/download_file?st=MTc0MDY0ODAyNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="5640568" href="https://unicaen.academia.edu/olivierlezoray">olivier lezoray</a><script data-card-contents-for-user="5640568" type="text/json">{"id":5640568,"first_name":"olivier","last_name":"lezoray","domain_name":"unicaen","page_name":"olivierlezoray","display_name":"olivier lezoray","profile_url":"https://unicaen.academia.edu/olivierlezoray?f_ri=3091","photo":"https://0.academia-photos.com/5640568/3197972/7756601/s65_olivier.lezoray.png"}</script></span></span></li><li class="js-paper-rank-work_4529814 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="4529814"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 4529814, container: ".js-paper-rank-work_4529814", }); 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We mainly focus on the first and the third steps here. A color watershed using global and local criteria is first described. A color contrast value is defined to select the best color space for segmenting color objects. Then, an architecture of binary neural networks is described. Its properties relies on the simplification of the recognition problem, leading to a noticeable increase in the classification rate. We conclude with the abilities of such a recognition scheme and present an automated cell sorting system.","downloadable_attachments":[{"id":49803417,"asset_id":4529814,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":5640568,"first_name":"olivier","last_name":"lezoray","domain_name":"unicaen","page_name":"olivierlezoray","display_name":"olivier lezoray","profile_url":"https://unicaen.academia.edu/olivierlezoray?f_ri=3091","photo":"https://0.academia-photos.com/5640568/3197972/7756601/s65_olivier.lezoray.png"}],"research_interests":[{"id":237,"name":"Cognitive Science","url":"https://www.academia.edu/Documents/in/Cognitive_Science?f_ri=3091","nofollow":true},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=3091","nofollow":true},{"id":10866,"name":"Morphology","url":"https://www.academia.edu/Documents/in/Morphology?f_ri=3091","nofollow":true},{"id":26066,"name":"Neural 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