PBS-15 Bioreactors | PBS Biotech

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started in the PBS-Minis and PBS-3. With a maximum working volume of 15L, the PBS-15 can accommodate larger scale process development or production for clinical trials.</p><ul role="list" class="product-benefits-list"><li>Continue scale up of process development or clinical stage production</li><li>Configurations available for use with single-use or reusable DO and pH sensors</li><li>Adjustable height harvest valve (AHHV) allows for rapid medium exchange, sampling, and harvesting</li><li>Touchscreen interface for intuitive process customization, control, and reporting</li></ul><a id="mini-01-shop-button" href="https://www.pbsbiotech.com/pbs-15-quote-request" class="mg-button mg-button-spacer w-button">Request a Quote</a></div><div class="mg-clear"></div></div></div></div><div class="section-resources"><div class="page-padding"><div class="conference-container"><h1 class="product-title-header product-resources-title">PBS-15 Vertical-Wheel Bioreactor Resources</h1><div data-current="FAQ" data-easing="ease" data-duration-in="300" data-duration-out="100" class="product-tab-container w-tabs"><div class="w-tab-menu"><a data-w-tab="Part Numbers" id="pbs--05-part-numbers" class="product-tab-link w-inline-block w-tab-link"><div>Part Numbers</div></a><a data-w-tab="Specifications" id="pbs-05-specifications" class="product-tab-link w-inline-block w-tab-link"><div>Specifications</div></a><a data-w-tab="Application Notes" id="pbs-05-app-notes" class="product-tab-link w-inline-block w-tab-link"><div>Application Notes</div></a><a data-w-tab="FAQ" id="pbs-05-faqs" class="product-tab-link w-inline-block w-tab-link w--current"><div>FAQ</div></a><a data-w-tab="Manuals" id="pbs-05-manuals" class="product-tab-link w-inline-block w-tab-link"><div>Manuals</div></a></div><div class="w-tab-content"><div data-w-tab="Part Numbers" class="w-tab-pane"><div class="w-embed"><style> #mini-15 { font-family: Karla; /* border: none; */ width: 100%; } td{ font-family: Karla; font-size: 16px; font-weight: 700; line-height: 24px; letter-spacing: 0px; text-align: left; } #mini-15 td, #mini-15 th { /* border: 1px solid #ddd; */ padding: 8px; } #mini-15 th { padding-top: 13px; padding-bottom: 12px; text-align: center; background-color: #233B5C; color: white; border: 1px solid #C8CCD8; border-left: none; } table, th, td { border: 1px solid #C8CCD8; border-collapse: collapse; } td { border: 1px solid #C8CCD8; } #mini td { background-color: White; } </style> <table id="mini-15"> <tbody><tr> <th>Product Type</th> <th>Product Name</th> <th>Product Number</th> </tr> <tr> <td class="bioreactors" rowspan="3">Bioreactors</td> <td>PBS-15 Vertical-Wheel Bioreactor (120 V) </td> <td>IA-15-B-501</td> </tr><tr><td> PBS-15 Vertical-Wheel Bioreactor (240 V) </td><td>IA-15-B-502 </td></tr> <tr><td> PBS-15 Vertical-Wheel Bioreactors, Single-Use Sensors (120 & 240 V) </td> <td> IA-15-B-701 </td> </tr> <tr> <td class="single" rowspan="3">Single-Use Vessels</td> <td>PBS-15 Vertical-Wheel Single-Use Vessel (Floored Wheel) </td> <td>IA-15-D-504-L</td> </tr><tr><td> PBS-15 Vertical-Wheel Single-Use Vessel (Floorless Wheel) </td><td> IA-15-D-506-L </td> </tr><tr><td> PBS-15 Vertical-Wheel Single-Use Vessels, Single-Use Sensors (Floored Wheel) </td><td> IA-15-D-704-L </td> </tr> <tr> <td class="Accessories" rowspan="3">Accessories</td> <td>PBS Autoclavable Probe Connector </td> <td>IA-UNI-DA-001 </td> </tr><tr><td> Reusable DO Sensor, 12mm OD x 220mm L with Right-Angle </td><td>IC00468 </td> </tr><tr><td> Reusable pH Sensor, 12mm OD x 225mm L </td> <td>IC00469 </td> </tr> <tr> <td class="software"> Software </td> <td> PBS-15 Vertical-Wheel Bioreactor Software 3.0 (21 CFR Part 11 cGMP Upgrade) </td> <td>IA-15-SW-001 </td> </tr> <tr> <td rowspan="2">Qualification Packages</td> <td> PBS-15 Vertical-Wheel Bioreactor IQ/OQ Protocols </td> <td>IA-15-BQ-004 </td> </tr><tr> <td> PBS-15 Vertical-Wheel Bioreactor IQ/OQ Configuration Sheet </td> <td>IA-15-BQ-005 </td> </tr> </tbody></table></div></div><div data-w-tab="Specifications" class="w-tab-pane"><div class="w-embed"><style> #mini-specifications-pbs-15 { font-family: Karla; /* border: none; */ width: 100%; } td{ font-family: Karla; font-size: 16px; font-weight: 700; line-height: 24px; letter-spacing: 0px; text-align: left; } #mini-specifications-pbs-15 td, #mini-specifications-pbs-15 th { /* border: 1px solid #ddd; */ padding: 8px; } #mini-specifications-pbs-15 th { padding-top: 13px; padding-bottom: 12px; text-align: center; background-color: #233B5C; color: white; border: 1px solid #C8CCD8; border-left: none; } table, th, td { border: 1px solid #C8CCD8; border-collapse: collapse; } td { border: 1px solid #C8CCD8; } #mini-specifications-pbs-15 td { background-color: White; } #mini-specifications-pbs-15 td.header-label { padding-top: .75rem; padding-bottom: .75rem; text-align: center; background-color: #233B5C; color: white; border: 1px solid #C8CCD8; border-left: none; width:20% } #mini-specifications-pbs-15 td.details-label { padding-left:1rem; } </style> <table style="margin-top:`1.25rem;" id="mini-specifications-pbs-15" cellspacing="0"> <tbody> <tr> <td class="header-label"> Working volume range:</td> <td class="details-label">9 – 15 L</td> </tr> <tr> <td class="header-label"> Gas control </td> <td class="details-label"> 4 mass flow controllers for air, N2, O2, CO2</td> </tr> <tr> <td class="header-label">Bag construction</td> <td class="details-label">Polyvinylidene Fluoride (PVDF)</td> </tr> <tr> <td class="header-label">Product contact materials</td> <td class="details-label">Meet requirements for USP Class VI Testing for Plastics <88> and/or ISO 10993 </td> </tr> </tbody></table></div><a id="mini-05-spec-button" href="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/67082abcf0f4caed8de2b674_PBS_Biotech_PBS15_Tech_Specs.pdf" target="_blank" class="mg-button mg-button-spacer w-button">Download Full Technical Spec Sheet</a></div><div data-w-tab="Application Notes" class="w-tab-pane"><div class="w-embed"><style> #mini-app-notes-15 { font-family: Karla; /* border: none; */ width: 100%; } td{ font-family: Karla; font-size: 16px; font-weight: 700; line-height: 24px; letter-spacing: 0px; text-align: left; } #mini-app-notes-15 td, #mini-app-notes-15 th { /* border: 1px solid #ddd; */ padding: 8px; } #mini-app-notes-15 th { padding-top: 13px; padding-bottom: 12px; text-align: center; background-color: #233B5C; color: white; border: 1px solid #C8CCD8; border-left: none; } table, th, td { border: 1px solid #C8CCD8; border-collapse: collapse; } td { border: 1px solid #C8CCD8; } #mini-app-notes-15 { background-color: White; } .product-app-note-button:link,.product-app-note-button:visited { float: right; clear: both; background-color: #f77c00; margin-left: 0; padding-left: 10px; padding-right: 10px; display: inline-block; color: #fff; border-color: #f77c00; border-radius: 3px; transition: all .2s linear; /* display: block; */ padding-top: 8px; padding-bottom: 8px; border: 1px solid #f77c00; } .product-app-note-button:hover { background-color: #fff; color:#f77c00; border: 1px solid #f77c00; } </style> <table style="margin-top:1.25rem;" id="mini-app-notes-15" cellspacing="0"> <tbody><tr> <th>Application Note Title</th> <th> </th> </tr> <tr> <td>Defined Biomatrices Accelerate MSC Manufacturing Across Scales</td> <td><a class="product-app-note-button" target="_blank" href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/6434a6a639b63b4b7a7ac231_2023%20Defined%20Biomatrices%20Accelerate%20MSC%20Manufacturing%20Across%20Scales.pdf">Read Now</a></td> </tr> <tr> <td>Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High-Quality hiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors</td> <td> <a class="product-app-note-button" target="_blank" href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63b68833b19e11c7b880dca7_2021%20Borys%20et%20al.%20-%20Overcoming%20Bioprocess%20Bottlenecks%20in%20Large-Scale%20Expansion.pdf">Read Now</a> </td> </tr> </tbody></table></div></div><div data-w-tab="FAQ" class="w-tab-pane w--tab-active"><div class="w-embed w-script"><style> table#mini-15-faq{ font-family: Karla; 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padding-left: 5px; padding-right: 5px; display: inline-block; color:#fff; border-color: #f77c00; bordeer-radius:5px; transition: all .2s linear; } .product-app-note-button:hover { background-color: #fff; color:#f77c00; } p.question { display:block; font-weight:bold; } p.answer { display:none; padding-top:1rem; } .question-answer-wrapper { position:relative; } img.question-one-icon, img.question-two-icon, img.question-three-icon, img.question-four-icon, img.question-five-icon,img.question-six-icon, img.question-seven-icon, img.question-eight-icon, img.question-nine-icon, img.question-ten-icon,img.question-eleven-icon { display:block; cursor:pointer; position: absolute; top:30%; left:50%; transform: translate(-50%, -90%) rotate(180deg); } img.answer-one-icon, img.answer-two-icon, img.answer-three-icon, img.answer-four-icon, img.answer-five-icon, img.answer-six-icon, img.answer-seven-icon, img.answer-eight-icon, img.answer-nine-icon, img.answer-ten-icon, img.answer-eleven-icon { display: none; cursor: pointer; position: absolute; top: 15%; left: 50%; transform: translate(-50%, -10%) rotate(0deg); } </style> <div style="max-width:800px; margin:0 auto"> <table style="margin-top:1.25rem;" id="mini-15-faq" cellspacing="0"> <tr> <td class="question-answer-detail-wrapper"><p class="question question-one">What cell types or cell culture applications are Vertical-Wheel bioreactors best suited for?</p> <p class="answer answer-one">Vertical-Wheel bioreactors provide the optimal hydrodynamic mixing environment for anchorage-dependent human cells that require suspension as large particles, such as cell aggregates or cells grown on microcarriers.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-one-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-one-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-two">What is the difference between floored and floorless Vertical-Wheel impellers and what kind of cell culture processes are each suited for?</p> <p class="answer answer-two">The floored version has more panels around the circumference of the Vertical-Wheel that create cup-like sections and is better suited for cells grown on microcarriers or as single cells. The floorless version is nearly identical but with less panels and allows more fluid flow towards the axle, and is better suited for cell aggregate applications. Both versions have nearly identical hydrodynamic performance.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-two-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-two-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-three">Are single-use dissolved oxygen and pH sensors available for use with all Vertical-Wheel bioreactors?</p> <p class="answer answer-three">The 3L, 15L, and 80L bioreactors are available in one version that accommodates single-use sensors and another version that is designed for reusable sensors (no cross-compatibility). The housing for the reusable sensor version can be modified to solely accommodate single-use sensors; please contact our Applications Engineering team for the service details.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-three-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-three-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-four">What is the Adjustable Height Harvest Valve (AHHV) and is it available in all Vertical-Wheel bioreactors?</p> <p class="answer answer-four">The AHHV extends from the bioreactor housing through the bottom of the single-use vessel using a sterile connection. Similar to a periscope, the tip of the AHHV can extend just beyond the top of a bed of settled cells to drain spent liquid with minimal supernatant for a medium exchange process, or can be used to harvest cells suspended in media. The AHHV is only available in 15L and larger bioreactors. More details can be found in respective User Manuals.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-four-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-four-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-five">How can I monitor process parameters during a cell culture run?</p> <p class="answer answer-five">The 3L bioreactor’s software allows for customizable programming of process controls and real-time monitoring of parameters using various sensors. Feedback loops can be used to pause a run based on deviations from a set range.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-five-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-five-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-six">Can I control and monitor my cell culture process remotely?</p> <p class="answer answer-six">Yes, the software on 3L and larger size bioreactors allows for remote process monitoring, adjustments, and alarm reporting over a secure connection to various devices.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-six-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-six-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-seven">How is process-related data stored and accessed?</p> <p class="answer answer-seven">The software can customize how often and what kind of data will be stored on the bioreactor’s on-board hard drive. Automatic downloading of data can be directed to a local computer with direct LAN connection. All user logins, process changes, and data histories follow 21 CFR Part 11 requirements for cGMP.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-seven-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-seven-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-eight">Can the 15L Vertical-Wheel bioreactor fit on top of a standard size lab bench?</p> <p class="answer answer-eight">The 15L bioreactor is recommended to be placed on the floor and has wheels for movement. Technical specifications such as dimensions and weight are available on the 15L product page.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-eight-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-eight-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-nine">How do I know the number and types of Vertical-Wheel bioreactors I will need?</p> <p class="answer answer-nine">Please use the Contact form to describe your process requirements and our Applications Engineering team will be able to assist with your planning.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-nine-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-nine-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-ten">What kind of training is needed to operate Vertical-Wheel bioreactors?</p> <p class="answer answer-ten">All components of PBS bioreactors (housing, vessels, and software) have been designed to be intuitive and easy to use even for those with no previous experience using bioreactors. The provided User Manual provides detailed instructions for common use cases and you can also Contact Us with any other questions.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-ten-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-ten-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> <tr> <td class="question-answer-detail-wrapper"><p class="question question-eleven">What if I have other questions regarding usage or troubleshooting?</p> <p class="answer answer-eleven">Please use the Contact form to reach out to our Applications Engineering team with any inquiries.</p></td> <td class="question-answer-icon-wrapper"> <img class="question-eleven-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> <img class="answer-eleven-icon" src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg"/> </td> </tr> </tbody></table> </div> <script src="https://ajax.googleapis.com/ajax/libs/jquery/3.7.1/jquery.min.js"></script> <script> //question 1 $(function(){ $(".question-one-icon").click(function(){ $(".question-one-icon").hide(function(){ $(".answer-one,.answer-one-icon").show("fast") }) }); 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}); </script></div></div><div data-w-tab="Manuals" class="w-tab-pane"><div class="w-embed"><style> #mini-manual-15 { font-family: Karla; /* border: none; */ width: 100%; } td{ font-family: Karla; font-size: 16px; font-weight: 700; line-height: 24px; letter-spacing: 0px; text-align: left; } #mini-manual-15 td, #mini-manual-15 th { /* border: 1px solid #ddd; */ padding: 8px; } #mini-manual-15 th { padding-top: 13px; padding-bottom: 12px; text-align: center; background-color: #233B5C; color: white; border: 1px solid #C8CCD8; border-left: none; } table, th, td { border: 1px solid #C8CCD8; border-collapse: collapse; } td { border: 1px solid #C8CCD8; } #mini-manual { background-color: White; } .product-app-note-button:link,.product-app-note-button:visited { float: right; clear: both; -webkit-text-stroke-color: var(--button-primary); background-color: #f77c00; margin-left: 0; padding-left: 5px; padding-right: 5px; display: inline-block; color:#fff; border-color: #f77c00; bordeer-radius:5px; transition: all .2s linear; } .product-app-note-button:hover { background-color: #fff; color:#f77c00; } </style> <table style="margin-top:1.25rem;" id="mini-manual-15" cellspacing="0"> <tbody><tr> <th>Title</th> <th> </th> </tr> <tr> <td>PBS-15 and PBS-80 Single-Use Bioreactors User Manual (Software v3) [P/Ns: IA-15-B-501 & -502; IA-80-B-511 & -512]</td> <td><a class="product-app-note-button" target="_blank" href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63bc44b078495ece665d2e5b_IC03771%20Rev%20D%20(digital).pdf">Read Now</a></td> </tr> <tr> <td>PBS-15 Datasheet</td> <td><a class="product-app-note-button" target="_blank" href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63c9e3f520944109b97e435b_15L%20Datasheet_print%20v.221007.pdf">Read Now</a> </td> </tr> </tbody></table></div></div></div></div></div></div></div><div class="section-service-card"><div class="page-padding"><div class="conference-container"><div class="feature-card-container"><div class="feature-card-wrapper"><div class="feature-card-image"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/6706b19e61223266dc3e459d_pbs-mini-feature-card-image.png" loading="lazy" sizes="300px" srcset="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/6706b19e61223266dc3e459d_pbs-mini-feature-card-image-p-500.png 500w, https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/6706b19e61223266dc3e459d_pbs-mini-feature-card-image.png 651w" alt="PBS-Mini Single-use bioreactor in the lab" class="responsive"/></div><div class="feature-card-detail"><h2 class="feature-card-title"> Need Help Maximizing the Yield and Quality of Your Cells?</h2><p class="featured-card-text">We can help! 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