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item */ ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hs-form-booleancheckbox-display span::after { content: ""; border: 2px solid #d9d9d9; border-radius: 3px; height: 20px; left: 0px; position: absolute; top: 0px; width: 20px; background: #fff; z-index: 0; display: block; transition-property: background-color, border-color, -webkit-box-shadow; -o-transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow, -webkit-box-shadow; } /* Show when checked */ ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::after, .hs-form-booleancheckbox-display input:checked + span::after, ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::before, .hs-form-booleancheckbox-display input:checked + span::before{ opacity: 1; } .hs-form-booleancheckbox-display input:checked+label:after, .hs-form-booleancheckbox-display input:checked+label:after { background: #fff; border-color: #157479; } /* Style when focused */ ul.inputs-list.multi-container li.hs-form-checkbox label input:focus + span::after, .hs-form-booleancheckbox-display input:focus + span::after { outline: 3px solid transparent; /* For Windows high contrast mode. */ box-shadow: 0 0 .3125rem rgba(0, 0, 0, .3); border-color: #157479; } .hs_contact_country{ clear: both; } .hs-button { border-radius: 65px !important; font-size: 14px; line-height: 1.3; letter-spacing: 1.12px; font-family: "Roboto Slab", helvetica, arial, sans-serif; font-weight: 700 !important; padding: 19px 23px !important; border: 2px solid; display: inline-block; text-decoration: none; text-transform: uppercase; -webkit-appearance: none !important; -moz-appearance: none !important; appearance: none !important; } .hbspt-form textarea{max-width: 100% !important; height: 186px !important; resize: none;} .hbspt-form textarea:focus{border: 1px solid #74cbc8 !important;} .hs-fieldtype-textarea .invalid.error{border: 2px solid #E74949 !important;} .hbspt-form fieldset{max-width: 100% !important; margin-bottom: 60px;} .hbspt-form .field {margin-bottom: 27px} .hs_recaptcha{ margin-top: 23px !important; margin-bottom: 27px !important; } .hs-button, .hs-form-field input[type=text], .hs-form-field input[type=email], .hs-form-field input[type=phone], .hs-form-field input[type=number], .hs-form-field input[type=tel], .hs-form-field input[type=date], .hs-form-field textarea { appearance: none; -webkit-appearance: none; -moz-appearance: none; } .hs-default-font-element, .hs-main-font-element { font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hbspt-form .hs-button { white-space: pre-wrap } .hbspt-form .hs-richtext { word-break: break-word } .hbspt-form.submitted-message { overflow: auto } .hbspt-form fieldset.form-columns-1 .hs-input { width: 95% } .hbspt-form fieldset.form-columns-1 .input { margin-right: 0px !important } .hbspt-form fieldset.form-columns-1 input[type=checkbox], .hbspt-form fieldset.form-columns-1 input[type=radio] { width: auto } .hbspt-form fieldset.form-columns-2 .hs-form-field { width: 50%; float: left } .hbspt-form fieldset.form-columns-2 .input { margin-right: 0px !important; } .hbspt-form fieldset.form-columns-3 .hs-form-field { width: 32.7%; float: left } .hbspt-form fieldset.form-columns-3 .input { margin-right: 8px } .hbspt-form label.hs-hidden { visibility: hidden } .hbspt-form .hs-field-desc { width: 100% } .hs-custom-style .hs-input, .hs-custom-style fieldset { max-width: 100% } .hs-custom-style>div.form-columns-3 .hs-form-field, .hs-custom-style fieldset.form-columns-3 .hs-form-field { width: 33.3% } .hs-custom-style>div>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]), .hs-custom-style fieldset>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]) { width: 100%; max-width: 100% } .hs-custom-style>div input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]), .hs-custom-style fieldset input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]) { box-sizing: border-box; padding: 0 15px; min-height: 27px } .hs-custom-style>div textarea, .hs-custom-style fieldset textarea { padding: 10px 15px } .hs-custom-style .hs-dependent-field>div .hs-input:not([type=checkbox]):not([type=radio]) { width: 100% } .legal-consent-container .field.hs-form-field { margin-bottom: 8px } .legal-consent-container .hs-field-desc.checkbox-desc { margin: -12px 0 0 21px } .legal-consent-container .hs-form-booleancheckbox-display input { float: left } .legal-consent-container .hs-form-booleancheckbox-display>span { display: block !important; margin-left: 30px !important; } .legal-consent-container .hs-form-booleancheckbox-display p { margin: 0; display: inline } .legal-consent-container .hs-error-msgs label { color: #f2545b } .legal-consent-container~.hs_recaptcha { margin-top: 18px } .cookie-reset-container { font-size: 14px; margin-bottom: 10px; text-align: right } #hs-outer-captcha-target, #hs-outer-captcha-target * { display: none; height: 0; width: 0 } .hubspot-link__container { font-size: 14px; padding-bottom: 40px; position: relative; color: #9fa0a2; font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hubspot-link-text { color: #00a4bd; font-weight: 400 } .hubspot-link__container.sproket { color: #9fa0a2 } .hubspot-link { color: #9fa0a2 } .hubspot-link, .hubspot-link:hover { text-decoration: none } .hubspot-link:hover .hubspot-link-text { text-decoration: underline } .hubspot-link__icon { margin-bottom: -1px; margin-right: 5px } .hubspot-link__container.sproket .hubspot-link__icon { width: 30px; margin-right: 0; float: left; margin-top: -9px; margin-left: -5px } .hubspot_signup_form .hs_email{margin-bottom: 0px !important; width: 80% !important; float: left !important;} .hubspot_signup_form .hs_submit{width: 20% !important; float: left !important; } .hubspot_signup_form .hs-button{padding: 12.5px 15px !important;} .hubspot_signup_form .hs_email label{display: none !important;} .hubspot_signup_form ul.no-list{position: absolute !important; top: 48px !important;} .hubspot_signup_form {position: relative !important;} .hubspot_signup_form .submitted-message{color: #ab8940 !important; font-size: 22px !important; } .hubspot_white .hbspt-form label, .hubspot_white .hs-form-required { color: #fff; } .hubspot_white .hs-input, .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hubspot_white .hs-form-booleancheckbox-display span::before{ background-color: #fff !important; } .hubspot_white .hs_submit { text-align: center; } .hubspot_white .hs-form select.is-placeholder { opacity: 1; } .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hubspot_white .hs-form-booleancheckbox-display span::after { border-color: #3f4852; } input[type=submit].hs-button.primary.large{ color: #fff; background: #00181e; border-color: #00181e !important; } input[type=submit].hs-button.primary.large:hover { background: #74cbc8 !important; border-color: #74cbc8 !important; } /***/ .hbspt-form .hs-input[type=checkbox]{ cursor: pointer; } .hs-form-booleancheckbox-display input:checked + span::after{ background: #fff; border-color: #157479; } .hbspt-form .form-columns-2 { display: flex; flex-flow: row wrap; -webkit-box-direction: normal; } .hbspt-form .hs_firstname { flex: 1; -webkit-box-flex: 1; } .hbspt-form .hs_submit{ margin-top: 60px; } .newsletter-form .hs_submit{margin-top: 45px;} .newsletter-form form .gfield_consent_description { border: none !important; } /*New Landing page*/ .landing-middle-section{ padding-right: 0px !important; } .left-col{max-width:93%} .left-col-para{padding-right:30px;} .left-col video{border-radius:4px;} /* Kit asssesment */ .kits_form_col .hbspt-form .hs-input[type=checkbox] { height: 20px !important; width: 20px !important; } .kits_form_col {padding-bottom: 45px;} .hs-richtext h4 {margin-bottom: 15px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 15px;} .kits_form_col .hbspt-form li.hs-form-checkbox label span{ margin-left: 0px !important; padding-left: 31px; } .hbspt-form label.hs-form-radio-display { position: relative; padding: 4px 31px; } .hbspt-form .hs-form-radio-display span, .hbspt-form .hs-form-checkbox-display span { position: relative; cursor: pointer; font-size: 15px; font-weight: 300; } .hbspt-form .hs-input[type=radio] { position: absolute; left: 0; z-index: 2; width: 20px !important; height: 20px !important; outline: none; cursor: pointer; background-color: transparent !important; border-radius: 50%; } .hbspt-form .hs_protocol_synopsis_upload .input{position: relative;} .hbspt-form input[type="file"]::-webkit-file-upload-button { cursor: pointer; background-color: #157479; padding: 10px 20px !important; border: 1px solid #157479; border-top-left-radius: 0px; border-bottom-left-radius: 0px; border-top-right-radius: 6px; border-bottom-right-radius: 6px; color: #fff; position: absolute; top: 0; bottom: 0; right: 0; } .hbspt-form input[type="file"] { cursor: pointer; padding: 10px 20px !important; position: relative; top: 0; bottom: 0; right: 0; } /*Form Responsive CSS*/ @media only screen and (min-width: 1024px) and (max-width: 1366px){ .hs_email, .hs_contact_country { clear: both; } } @media only screen and (min-width: 992px) and (max-width: 1024px){ .hs_email, .hs_contact_country { clear: both; } } @media (min-width: 640px) and (max-width: 1120px) { .hbspt-form h1{font-size: 36px !important;} } @media screen and (min-width: 1120px) { .hbspt-form h1{font-size: 45px !important;margin-bottom: 17px !important;} .hbspt-form .gform_heading p{font-size: 17px !important;} } @media only screen and (min-width: 768px) and (max-width: 992px){ .hs_email, .hs_contact_country{ clear: both; } } @media (min-width: 767px) and (max-width: 1024px) { body .hubspot_signup_form .hs_email{width: 66% !important; float: left !important;} } @media screen and (max-width: 992px){ .assay-kit-hbspt-form .fl-col { width: 100%; } } @media screen and (max-width:812px){ .assay-kit-hbspt-form .fl-col { max-width: 100%; } .hbspt-form .hs-fieldtype-select .input:before{ right: 20px !important; } } @media screen and (max-width: 767px){ .hs_submit .actions{padding-top: 0px !important; margin-top: 0px !important;} body .hbspt-form fieldset.form-columns-2 .input {margin-right: 0px !important;} body fieldset.form-columns-3 .input{margin-right: 0px !important;} .hs-form-field .hs-input { width: 100% !important; } .hs-fieldtype-textarea .input{margin-right: 0px !important;} .hs_firstname, .hs_phone, .hs_development_area{ width: 100% !important; } } @media screen and (min-width: 640px){ .hbspt-form .hs_firstname, .kits_form_col .hs_email{ 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class="breadcrumbs"><span><span><a href="https://www.bioagilytix.com/"><i class="home-icon"></i><span class="screen-reader-text">Home</span></a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/">Techniques & Platforms</a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/instrumentation/">Instrumentation</a></span> <span class="breadcrumb-separator"></span> <span class="breadcrumb_last" aria-current="page">ImmunoCAP</span></span></div></div></section><div class="wp-block-group alignwide is-layout-constrained wp-container-core-group-is-layout-1 wp-block-group-is-layout-constrained"><p class="has-text-align-center has-large-font-size">The ImmunoCAP platform is used to measure total or specific IgG and IgE antibodies in samples. BioAgilytix offers ImmunoCAP assay development and testing services at our laboratory in Hamburg, Germany, to deliver reproducible results. <strong> </strong></p></div><div id="block_00b23b52e7e6e28b572e761f95e08681" class="wp-block em-media-content em-mt--none em-mb--lg alignfull"><div class="em-media-content__inner em-media-content__inner--media-right has-gray-xxl-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--right"> <img fetchpriority="high" decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/immuno-cell-1380x776.jpg" alt="immuno cell rendering" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/immuno-cell-1380x776.jpg 1380w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immuno-cell-1120x630.jpg 1120w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immuno-cell-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immuno-cell-640x360.jpg 640w" width="1380" height="776"></div><div class="em-media-content__content em-media-content__content--left em-media-content__content--add-padding"><h2 class="h2"><strong><strong>BioAgilytix鈥檚 ImmunoCAP platform expertise  </strong></strong></h2><p class="">Our scientific team has proven their ability to efficiently, consistently perform assays using ImmunoCAP, and to develop robust assays used to produce timely results. We have vast experience leveraging this platform to develop custom assays for determining drug-specific IgE and IgG responses for understanding immunogenicity. </p></div></div></div><div id="block_859a61fd8c18e1b348c8e3a59e584f8b" class="wp-block em-media-content alignwide"><div class="em-media-content__inner em-media-content__inner--media-left has-none-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--left"> <noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364.jpg" alt="researcher looking at vial" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-300x169.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-768x432.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-640x360.jpg 640w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-100x56.jpg 100w" width="1000" height="563"></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%201000%20563%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364.jpg" alt="researcher looking at vial" class="lazyload object-fit--cover" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-300x169.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-768x432.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-640x360.jpg 640w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1924516364-100x56.jpg 100w" width="1000" height="563"></div><div class="em-media-content__content em-media-content__content--right em-media-content__content--add-padding"><h6 class="has-primary-l-color has-text-color h6"><strong><strong>Key ImmunoCAP Benefits: </strong></strong></h6><h2 class="h2"><strong><strong>Automated processing and reproducible results </strong></strong></h2><p class="">The key advantages of the ImmunoCAP platform include its ability to enable high productivity, high sensitivity, and high drug tolerance. Using automated processing, the platform can also provide standardized and reproducible results. </p><p class="">Designed as sandwich assays, the solid phase of ImmunoCAP ensures binding of all relevant antibodies. This is achieved through an extreme binding capacity on the ImmunoCAP surface. We use ImmunoCAP assays to deliver accurate and reproducible results with increased efficiency and shortened turnaround times, achieved with the platform鈥檚 high-throughput automation capabilities. </p><p class="">Benefits of the ImmunoCAP platform include: </p><ul class="wp-block-list"><li class="">High sensitivity </li><li class="">High specificity </li><li class="">Standardized, reproducible results </li><li class="">Automated processing </li></ul></div></div></div><div class="wp-block-emagine-background alignfull has-quaternary-background-color has-background-dim em-pt--xl em-pb--xl em-mt--lg"><div class="wp-block-emagine-background__content"><div class="wp-block-emagine-background__content-inner"><div class="wp-block-emagine-grid alignwide wp-block-emagine-grid--halves"><div class="wp-block-emagine-grid__inner em-grid-2_sm-1-lgGutter-left-middle"><div class="wp-block-emagine-column em-col-middle"><h6 class="has-primary-l-color has-text-color h6"><strong>Ideal ImmunoCAP Applications: </strong></h6><h2 class="has-em-white-color has-text-color h2"><strong>For IgE-mediated hyperreactivity analysis </strong></h2><p class="">ImmunoCAP offers very sensitive IgE detection in the low ng- or pg range with high precision. It can be used to determine IgE mediated immunogenicity and enables IgE antibody detection at an early stage, indicating sensitization鈥攅ven before clinical symptoms have developed. </p><p class="">BioAgilytix leverages the use of the ImmunoCAP platform to measure anti-drug IgE antibodies in samples for immunogenicity studies.  </p></div><div class="wp-block-emagine-column em-col-middle"><div class="wp-block-image size-full"><div><figure class=""><noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/06/Antibody@1x.png" alt="" class="wp-image-380"/></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%20210%20140%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/06/Antibody@1x.png" alt="" class="lazyload wp-image-380"/></figure></div></div></div></div></div></div></div></div><div id="block_b457a8a312e2b56a0256eed256f21266" class="wp-block em-media-content em-mt--md em-mb--md alignwide"><div class="em-media-content__inner em-media-content__inner--media-left has-none-background-color"><div class="em-media-content__media em-media-content__media--left"> <noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/immunocap.jpg" alt="immunocap" class="object-fit--contain" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/immunocap.jpg 450w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunocap-300x229.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunocap-100x76.jpg 100w" width="450" height="343"></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%20450%20343%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/immunocap.jpg" alt="immunocap" class="lazyload object-fit--contain" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/immunocap.jpg 450w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunocap-300x229.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunocap-100x76.jpg 100w" width="450" height="343"></div><div class="em-media-content__content em-media-content__content--right"><h3 class="h3"><strong><strong><strong>How the ImmunoCAP platform works  </strong></strong></strong></h3><p class="">The technology used in the ImmunoCAP platform enables a high binding capacity per mg cellulose in combination with an optimal amount of cellulose in each solid phase. This ensures binding of all relevant antibodies, regardless of antibody affinity, still giving low non-specific binding. The ImmunoCAP solid phase consists of a cellulose derivative enclosed in a capsule. The hydrophilic, highly branched polymer provides an ideal microenvironment for binding proteins. </p><p class="">The test is designed as a sandwich immunoassay. Biotinylated drug, bound to streptavidin-coated solid phase, reacts with the drug-specific IgE in the patient serum sample. After washing, enzyme-labeled antibodies against IgE or IgG are added to form a complex. After incubation, unbound enzyme labeled antibody is washed away, and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. The fluorescence is directly proportional to the concentration of IgE or IgG in the serum sample.</p></div></div></div><div id="block_56776e711c8b002da6e18b1ab01e4bd4" class="wp-block em-call-to-action em-mt--xl em-mb--none alignfull"><div class="em-call-to-action--align-center has-primary-l-background-color has-background-opacity-- em-pt--lg em-pb--lg em-pl--xl em-pr--xl"><div class="em-call-to-action__inner"><p class="">Blog</p><h2 class="h2">Platform spotlight: ImmunoCAP to support diagnosis of allergic reactions against biologics</h2><div class="wp-block-emagine-button has-text-align-left is-style-arrow-right"><a class="btn btn--size-md btn--style-primary has-quaternary-background-color" href="https://www.bioagilytix.com/2019/07/25/platform-spotlight-immunocap-to-support-diagnosis-of-allergic-reactions-against-biologics/" target="_self" rel="noopener"><span>Read <span class="btn__last-word">more</span></span></a></div></div></div></div></article></main><footer class="site-footer 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