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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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aem-GridColumn--tablet--12 aem-GridColumn--offset--tablet--0 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn--tablet--none aem-GridColumn--default--9 aem-GridColumn aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-e06ec823b7" class="cmp-container"> <div class="cmp-p-container p-l1"> <div id="container-53adc83b52" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-ctaitem mb-l1 aem-GridColumn aem-GridColumn--default--12"><div class="cmp-ctaitem" id="ctaitem-0749d373a5"> <a class="cmp-ctaitem__anchor" href="/sg/en/home/global/forms/life-science/novafluor-dyes-request.html"> <span class="cmp-ctaitem__text">Learn more about our NovaFluor sample program for your lab</span> </a> </div> </div> <div class="cmp-p-text mt-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="text-391223895" class="cmp-text"> <p><a></a>NovaFluor dyes are designed with narrow excitation for minimal cross-laser excitation for spectral and traditional flow cytometry to deliver high resolution. Lower spectral spillover or overlap lessens the need for compensation, decreases spreading error, and increases opportunities to add new markers. This aids in construction of flow cytometry panels with increased resolution while expanding the overall size of panels. NovaFluor conjugated antibodies represent foundational technology in the rapidly expanding world of flow cytometry. <a></a><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/spectral-flow-cytometry-assays-reagents.html">Learn more about our total spectral flow cytometry offerings.</a><br /> <br /> </p> <p>Explore more about the advantages of using NovaFluor dyes:</p> <ul> <li><a href="#laser">Minimalized cross-laser excitation</a></li> <li><a href="#compensation">Minimalized impact to compensation</a></li> <li><a href="#spillover">Decreased spillover spread</a></li> <li><a href="#expansion">Designed for panel expansion</a></li> </ul> <p>And to enhance NovaFluor data resolution by blocking dye-to-cell interactions, use <a data-uw-rm-kbnav="anohref"></a><a href="#cellblox" data-uw-rm-brl="false">Invitrogen CellBlox Plus Blocking Buffer</a>.</p> </div> </div> </div> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-c00fe6165a"> <div class="cmp-sectiontitle"> <h2 id="guide" class="cmp-sectiontitle__text" data-anchor-nav-title="NovaFluor Dye Selection Guide ">NovaFluor dye selection guide </h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-30b5fcb518" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-553424902" class="cmp-text"> <p>Use the table below to select a NovaFluor dye and see available conjugated antibodies. Check stain index for brightness <a href="https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/flow-cytometry-stain-index-graphic.pdf" target="_blank">here</a></p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-e787007531" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-text aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12"> <table class="rte-forcedborder" border="0" width="100%" cellspacing="0" cellpadding="0"> <tbody><tr><td style="background-color: #808080; color: #fff; width: 20%;"><p><b>Ultra Violet<br> (Ex/Em max)</b></p> </td> <td style="background-color: #8200c8; color: #fff; width: 20%;"><p><b>Violet<br> (Ex/Em max)</b></p> </td> <td style="background-color: #0000ff; color: #fff; width: 20%;"><p><b>Blue<br> (Ex/Em max)</b></p> </td> <td style="background-color: #ffff00; width: 20%;"><p><b>Yellow<br> (Ex/Em max)</b></p> </td> <td style="background-color: #ff0000; width: 20%; color: #fff;"><p><b>Red</b><br> <b>(Ex/Em max)</b></p> </td> </tr><tr><td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-uv-765.html">NovaFluor UV 765*</a></p> <p>(358/763 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-violet-690.html">NovaFluor Violet 690*</a></p> <p>(408/691 nm)</p> </td> <td><p><a href="/antibody/primary/query/*/filter/conjugates/NovaFluor%20Blue%20510">NovaFluor Blue 510</a></p> <p>(496/511 nm)</p> </td> <td><p><a href="/antibody/primary/query/*/filter/conjugates/NovaFluor%20Yellow%20570">NovaFluor Yellow 570</a></p> <p>(552/568 nm)</p> </td> <td><p><a href="/antibody/primary/query/*/filter/conjugates/NovaFluor%20Red%20660">NovaFluor Red 660</a></p> <p>(637/659 nm)</p> </td> </tr><tr><td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-violet-745.html">NovaFluor Violet 745*</a></p> <p>(391/743 nm)</p> </td> <td><p><a href="/antibody/primary/query/*/filter/conjugates/NovaFluor%20Blue%20530">NovaFluor Blue 530</a></p> <p>(509/530 nm)</p> </td> <td><p><a href="/antibody/primary/query/*/filter/conjugates/NovaFluor%20Yellow%20590">NovaFluor Yellow 590</a></p> <p>(552/590 nm)</p> </td> <td><p><a href="/antibody/primary/query/*/filter/conjugates/NovaFluor%20Red%20685">NovaFluor Red 685</a></p> <p>(637/685 nm)</p> </td> </tr><tr><td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-violet-800.html">NovaFluor Violet 800*</a></p> <p>(408/800 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-555.html">NovaFluor Blue 555</a></p> <p>(494/555 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-yellow-610.html">NovaFluor Yellow 610</a></p> <p>(552/612 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-red-700.html">NovaFluor Red 700</a></p> <p>(639/700 nm)</p> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-585.html">NovaFluor Blue 585</a></p> <p>(494/585nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-yellow-660.html">NovaFluor Yellow 660</a></p> <p>(552/663 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-red-700.html">NovaFluor Red 710</a></p> <p>(639/710 nm)</p> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-610-30s.html">NovaFluor Blue 610-30S</a></p> <p>(509/614 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-yellow-690.html">NovaFluor Yellow 690</a></p> <p>(552/690 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-red-725.html">NovaFluor Red 725</a></p> <p>(636/725 nm)</p> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-610-70s.html">NovaFluor Blue 610-70S</a></p> <p>(509/614 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-yellow-700.html">NovaFluor Yellow 700</a></p> <p>(552/700 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-red-755.html">NovaFluor Red 755</a></p> <p>(636/755 nm)</p> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-660-40s.html">NovaFluor Blue 660-40S</a></p> <p>(509/665 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-yellow-730.html">NovaFluor Yellow 730</a></p> <p>(552/731 nm)</p> </td> <td> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-660-120s.html">NovaFluor Blue 660-120S</a></p> <p>(509/665 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-yellow-755.html">NovaFluor Yellow 755</a></p> <p>(552/755 nm)</p> </td> <td> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-690.html">NovaFluor Blue 690</a></p> <p>(494/690 nm)</p> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-yellow-810.html">NovaFluor Yellow 810*</a></p> <p>(551/813 nm)</p> </td> <td> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-725.html">NovaFluor Blue 725*</a></p> <p>(492/725 nm)</p> </td> <td> </td> <td> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-760.html">NovaFluor Blue 760</a></p> <p>(490/764 nm)</p> </td> <td> </td> <td> </td> </tr><tr><td> </td> <td> </td> <td><p><a href="/sg/en/home/life-science/cell-analysis/fluorophores/novafluor-blue-800.html">NovaFluor Blue 800*</a></p> <p>(493/806 nm)</p> </td> <td> </td> <td> </td> </tr> <tr> <td colspan="5" style="text-align: left;">  <p>*This NovaFluor dye fits in a spectral space that is currently not accessible by other spectral dyes.</p> </td> </tr> </tbody></table> </div> <div class="cmp-p-text mt-l1 aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12"> <div id="text-3294130" class="cmp-text"> <p>Have an antibody that isn’t conjugated to a NovaFluor? <a href="/order/catalog/product/K06T04L032">Use our NovaFluor conjugation kits to create NovaFluor conjugates on-demand</a>.</p> <p> </p> <p>For more ultra violet and violet dye options explore <a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/brilliant-polymer-dyes.html">Brilliant Ultra Violet™/Violet™ Polymer Dyes</a> and <a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/super-bright-polymer-dyes.html">Super Bright Polymer dyes</a>.</p> </div> </div> </div> </div> </div> <div class="cmp-p-container m--no-gutters p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-f0622ce0fb" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> </div> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-05d90f46c5"> <div class="cmp-sectiontitle"> <h2 id="laser" class="cmp-sectiontitle__text" data-anchor-nav-title="Minimalized Cross-Laser Excitation ">Minimalized cross-laser excitation </h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-a6debb0b05" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1506652271" class="cmp-text"> <p><a id="laser"></a>Cross-laser excitation is a significant contributor to spillover in flow cytometry experiments. Some fluorophores have excitation curves that enable fluorescence on more than one laser, leading to excess spillover and consequent spreading error. These effects can reduce the maximum experiment complexity possible on any instrument. NovaFluor dyes are designed to minimize cross-laser excitation, thereby enabling the full potential of your flow cytometer.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-ea62115e0e" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-accordion aem-GridColumn aem-GridColumn--default--12"><div id="accordion-30bf03de06" class="cmp-accordion" data-cmp-is="accordion"> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-30bf03de06-item-cc4e4d3c9c"> <h2 class="cmp-accordion__header"> <button id="accordion-30bf03de06-item-cc4e4d3c9c-button" class="cmp-accordion__button" aria-controls="accordion-30bf03de06-item-cc4e4d3c9c-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">See minimal cross-laser excitation </span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-30bf03de06-item-cc4e4d3c9c-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-30bf03de06-item-cc4e4d3c9c-button"><div class="cmp-p-container"> <div id="container-cc4e4d3c9c" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-719f0c05a6" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1243456165" class="cmp-text"> <p>A closer look at the excitation curve for PE. PE is well-excited by blue (488 nm), green (532 nm), and yellow-green (561 nm) laser excitation sources. This results in the fluorophore having cross-laser excitation. NovaFluor dyes have much narrower excitation curves optimized for the blue and yellow-green lasers most common on flow cytometers. Using NovaFluor dyes resolves the cross-laser excitation issues.</p> <p> </p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-d97bb3b3ec" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/Novafluor-vs-PE-Dazzle-r.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/Novafluor-vs-PE-Dazzle-r.jpg" data-title="Graph showing excitation spectrum" id="image-1160370580" class="cmp-image hand-icon" itemscope itemtype="http://schema.org/ImageObject"> <div data-toggle="modal" data-target="#modal-image-1160370580" class="modal-toggle"> <img src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/Novafluor-vs-PE-Dazzle-r.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph showing excitation spectrum"/> </div> </div>  <div class="cmp-image-modal modal" id="modal-image-1160370580" tabindex="-1" role="dialog" aria-labelledby="modal-label-for-image-1160370580"> <div class="cmp-image-modal__body modal-body"> <div class="cmp-image-modal__header modal-header no-border"> <button type="button" class="cmp-image-modal__close close" data-dismiss="modal" aria-label="Close"> <span class="cmp-image-modal__close__icon" aria-hidden="true">×</span> </button> </div>  <img loading="lazy" src="/content/dam/tfsite/images/storefront/Novafluor/Novafluor-vs-PE-Dazzle.jpg" class="cmp-image-modal__body__image" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph showing excitation spectrum"/> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-6720e2b4d4" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-656235195" class="cmp-text"> <p><b>Figure 1. Excitation ranges of NovaFluor dyes compared to PE/Dazzle 594 dye. </b>Excitation spectrum of PE/Dazzle 594 (left) and normalized excitation spectra of PE/Dazzle 594, NovaFluor Blue 610, and NovaFluor Yellow 610 (right) with blue (488 nm), green (532 nm), yellow (561 nm), and red (635 nm) laser lines overlaid. While PE/Dazzle 594 is well-excited by blue, green and yellow lasers, NovaFluor dyes exhibit narrower excitation ranges, so swapping PE/Dazzle with NovaFluor Blue 610 and NovaFluor Yellow 610 frees an additional channel for adding another marker. </p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-d40273888f" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-ctaitem m--btn-link m--center aem-GridColumn--offset--default--11 aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--1"><div class="cmp-ctaitem" id="ctaitem-3aed42660f"> <a class="cmp-ctaitem__anchor" href="#top"> <span class="cmp-ctaitem__text">Top</span> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer mt-l2 m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-b945406182"> <div class="cmp-sectiontitle"> <h2 id="compensation" class="cmp-sectiontitle__text" data-anchor-nav-title="Minimalized Impact to Compensation ">Minimalized impact to compensation</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-c33c1fa266" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-712467738" class="cmp-text"> <p><a id="compensation"></a>Increasing panel complexity can lead to compensation-related background. NovaFluor dyes are designed to minimize impacts to compensation, making it easier to increase panel size and complexity with minimal impact to compensation.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-3feb4febfd" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-accordion aem-GridColumn aem-GridColumn--default--12"><div id="accordion-739e3d5523" class="cmp-accordion" data-cmp-is="accordion"> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-739e3d5523-item-d500c7a907"> <h2 class="cmp-accordion__header"> <button id="accordion-739e3d5523-item-d500c7a907-button" class="cmp-accordion__button" aria-controls="accordion-739e3d5523-item-d500c7a907-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">See minimal impact to compensation </span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-739e3d5523-item-d500c7a907-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-739e3d5523-item-d500c7a907-button"><div class="cmp-p-container"> <div id="container-d500c7a907" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-40393db12e" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-895435986" class="cmp-text"> <p>Typically, researchers do not combine PerCP-Cy5.5 and PE-Cy5.5 in the same panel due to spillover and compensation errors. Data here demonstrate compensation errors observed when using the tandem dyes PerCP-Cy5.5 and PE-Cy5.5 together in a panel collected on a default Attune cytometer setup (top row). Spillover from PerCp-Cy5.5 into the YL3 channel results in spreading of the PerCP-Cy5.5 negative populations, resulting in populations that do not resolve properly. Incorrect compensation can also lead to downstream analysis errors, as shown in the CD45RA+CD45RO+ population (top row) that does not exist when the CD4 and CD8 populations are separated properly (bottom row) using NovaFluor dyes. NovaFluor dyes that occupy the same primary channels as PerCp-Cy5.5 and PE-Cy5.5 (BL3 and YL3) can clearly resolve cellular populations in our T cells panel, allowing for proper downstream analysis and expanded panel selection.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-e5de23a529" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/small-panel-convention-swap-r.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/small-panel-convention-swap-r.jpg" data-title="Graph showing differences in compensation errors" id="image-1198723844" class="cmp-image hand-icon" itemscope itemtype="http://schema.org/ImageObject"> <div data-toggle="modal" data-target="#modal-image-1198723844" class="modal-toggle"> <img src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/small-panel-convention-swap-r.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph showing differences in compensation errors"/> </div> </div>  <div class="cmp-image-modal modal" id="modal-image-1198723844" tabindex="-1" role="dialog" aria-labelledby="modal-label-for-image-1198723844"> <div class="cmp-image-modal__body modal-body"> <div class="cmp-image-modal__header modal-header no-border"> <button type="button" class="cmp-image-modal__close close" data-dismiss="modal" aria-label="Close"> <span class="cmp-image-modal__close__icon" aria-hidden="true">×</span> </button> </div>  <img loading="lazy" src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/small-panel-convention-swap-1375x1100.jpg" class="cmp-image-modal__body__image" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph showing differences in compensation errors"/> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-000ad6d5af" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-643295524" class="cmp-text"> <table border="1" cellspacing="0" cellpadding="0" width="99%"> <tbody><tr><td width="177"><p><strong>Set up (5-color panel) with conventional tandem dyes</strong></p> </td> <td width="153"><p><strong>Fluorophore</strong></p> </td> <td width="135"><p><strong>Antigen</strong></p> </td> <td width="153"><p><strong>Set up (6-color panel) with NovaFluor dyes</strong></p> </td> <td width="153"><p><strong>Fluorophore</strong></p> </td> <td width="135"><p><strong>Antigen</strong></p> </td> </tr><tr><td width="177"><p>V1</p> </td> <td width="173"><p>Live/Dead Violet</p> </td> <td width="135"><p>Viability</p> </td> <td width="153"><p>V1</p> </td> <td width="173"><p>Live/Dead Violet</p> </td> <td width="135"><p>Viability</p> </td> </tr><tr><td width="177"><p>V2</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> <td width="153"><p>V2</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> </tr><tr><td width="177"><p>V3</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> <td width="153"><p>V3</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> </tr><tr><td width="177"><p>V4</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> <td width="153"><p>V4</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> </tr><tr><td width="177"><p>B1</p> </td> <td width="173"><p>FITC</p> </td> <td width="135"><p>CD3</p> </td> <td width="153"><p>B1</p> </td> <td width="173"><p>FITC</p> </td> <td width="135"><p>CD3</p> </td> </tr><tr><td width="177"><p>B2</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> <td width="153"><p>B2</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> </tr><tr><td width="177"><p>B3</p> </td> <td width="173"><p>PerCp Cy5.5</p> </td> <td width="135"><p>CD4</p> </td> <td width="153"><p>B3</p> </td> <td width="173"><p>NovaFluor Blue 660-120S </p> </td> <td width="135"><p>CD4</p> </td> </tr><tr><td width="177"><p>Y1</p> </td> <td width="173"><p>PE</p> </td> <td width="135"><p>CD45RA</p> </td> <td width="153"><p>Y1</p> </td> <td width="173"><p>PE </p> </td> <td width="135"><p>CD45RA</p> </td> </tr><tr><td width="177"><p>Y2</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> <td width="153"><p>Y2</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> </tr><tr><td width="177"><p>Y3</p> </td> <td width="173"><p>PE Cy5.5</p> </td> <td width="135"><p>CD8</p> </td> <td width="153"><p>Y3</p> </td> <td width="173"><p>NovaFluor Yellow 660</p> </td> <td width="135"><p>CD8</p> </td> </tr><tr><td width="177"><p>Y4</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> <td width="153"><p>Y4</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> </tr><tr><td width="177"><p>R1</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> <td width="153"><p>R1</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> </tr><tr><td width="177"><p>R2</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> <td width="153"><p>R2</p> </td> <td width="173"><p> </p> </td> <td width="135"><p> </p> </td> </tr><tr><td width="177"><p>R3</p> </td> <td width="173"><p>APC-eFluor 780</p> </td> <td width="135"><p>CD45RO</p> </td> <td width="153"><p>R3</p> </td> <td width="173"><p>APC-eFluor 780</p> </td> <td><p>CD45RO</p> </td> </tr></tbody></table> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-5e87fbbe6d" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-2074182601" class="cmp-text"> <p><b>Figure 2. Use of NovaFluor dyes effectively corrects the compensation errors seen when using conventional fluorophores.</b> Normal human peripheral blood mononuclear cells (1 x 106 cells/well) were stained in 1x PBS using 1 µL of <a href="/order/catalog/product/L34955" data-uw-rm-brl="false">LIVE/DEAD Fixable Violet Dead Cell Stain Kit for 405nm excitation</a> (Cat. No. L34955) for 30 minutes. Cells were washed and then blocked using <a href="/order/catalog/product/B001T03F01" data-uw-rm-brl="false">CellBlox Blocking Buffer</a> (Cat. No. B001T03F01) and Fc Receptor Binding Inhibitor Polyclonal Antibody (Cat. No. 14-9161-71) in <a href="/order/catalog/product/00-4222-26" data-uw-rm-brl="false">eBioscience Flow Cytometry Staining Buffer</a> (Cat. No. 00-4222-26). Cells were then stained in two separate panels, one using <a href="/antibody/product/CD4-Antibody-clone-RPA-T4-Monoclonal/45-0049-42" data-uw-rm-brl="false">CD4 Monoclonal Antibody (RPA-T4), PerCP-Cyanine5.5</a> (5 µL, Cat. No. 45-0049-42) and <a href="/antibody/product/CD8a-Antibody-clone-RPA-T8-Monoclonal/35-0088-42" data-uw-rm-brl="false">CD8a Monoclonal Antibody (RPA-T8), PE-Cyanine5.5</a> (5 µL, Cat. No. 35-0088-42), and the other replacing PerCp-Cy5.5 with <a href="/antibody/product/CD4-Antibody-clone-SK3-SK-3-Monoclonal/H001T03B08" data-uw-rm-brl="false">CD4 Monoclonal Antibody (SK3 (SK-3)) NovaFluor Blue 660-120S</a> (5 µL, Cat. No. H001T03B08) and replacing PE-Cy5.5 with <a href="/antibody/product/CD8a-Antibody-clone-OKT8-OKT-8-Monoclonal/H003T03Y04" data-uw-rm-brl="false">CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Yellow 660</a> (5 µL, Cat. No. H003T03Y04). Cells were stained for 30 minutes at 4°C, washed, and then fixed using 100 µL of eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) for 30 minutes. Cells were then washed, and data was collected on an <a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/attune-nxt-flow-cytometer/models/cytpix.html" data-uw-rm-brl="false">Attune CytPix Flow Cytometer </a>(4-laser configuration) from 30,000 cells from the lymphocyte gate. Collected data was compensated and later analyzed using FlowJo® software.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-c14ff98746" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-ctaitem m--btn-link m--center aem-GridColumn--offset--default--11 aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--1"><div class="cmp-ctaitem" id="ctaitem-f0f1e66540"> <a class="cmp-ctaitem__anchor" href="#top"> <span class="cmp-ctaitem__text">Top</span> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer mt-l2 m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-e30c350931"> <div class="cmp-sectiontitle"> <h2 id="spillover" class="cmp-sectiontitle__text" data-anchor-nav-title="Decreased Spillover Spread ">Decreased spillover spread </h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-d7c767880c" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-724186391" class="cmp-text"> <p><a id="spillover"></a>Spillover spread is impacted by several factors including fluorophore emission-spectra overlap, antigen expression level, and cross-laser excitation. Fluorophores with high cross-laser excitation can increase spread to a level where a detector will no longer be usable due to excessive background.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-ed20599add" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-accordion aem-GridColumn aem-GridColumn--default--12"><div id="accordion-48cb817935" class="cmp-accordion" data-cmp-is="accordion"> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-48cb817935-item-8244c9799b"> <h2 class="cmp-accordion__header"> <button id="accordion-48cb817935-item-8244c9799b-button" class="cmp-accordion__button" aria-controls="accordion-48cb817935-item-8244c9799b-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">See decreased spillover</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-48cb817935-item-8244c9799b-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-48cb817935-item-8244c9799b-button"><div class="cmp-p-container"> <div id="container-8244c9799b" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-7127bde917" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-628680189" class="cmp-text"> <p>Fluorophores designed to avoid cross-laser excitation can be valuable in the expansion of usable detectors on a flow cytometry instrument. NovaFluor dye conjugated antibodies display lower cross-laser spillover in comparison to the conventional PE tandem dye conjugated antibodies.<b></b></p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-9d8c6d484b" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/spillover-data-r.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/spillover-data-r.jpg" data-title="Novafluor vs conventional dye spillover" id="image-1903831949" class="cmp-image hand-icon" itemscope itemtype="http://schema.org/ImageObject"> <div data-toggle="modal" data-target="#modal-image-1903831949" class="modal-toggle"> <img src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/spillover-data-r.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Novafluor vs conventional dye spillover"/> </div> </div>  <div class="cmp-image-modal modal" id="modal-image-1903831949" tabindex="-1" role="dialog" aria-labelledby="modal-label-for-image-1903831949"> <div class="cmp-image-modal__body modal-body"> <div class="cmp-image-modal__header modal-header no-border"> <button type="button" class="cmp-image-modal__close close" data-dismiss="modal" aria-label="Close"> <span class="cmp-image-modal__close__icon" aria-hidden="true">×</span> </button> </div>  <img loading="lazy" src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/spillover-data-v2-640x640.jpg" class="cmp-image-modal__body__image" itemprop="contentUrl" data-cmp-hook-image="image" alt="Novafluor vs conventional dye spillover"/> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-1af1d075b8" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1300118408" class="cmp-text"> <p><strong>Figure 3. Spillover reduction using NovaFluor antibodies to replace conventional tandem dyes.</strong> <a data-uw-rm-kbnav="anohref"></a><a href="/order/catalog/product/01-3333-42">UltraComp eBeads Plus Compensation Beads </a>(Cat. No. 01-3333-42) were single stained with either <a data-uw-rm-kbnav="anohref"></a><a href="/antibody/product/CD4-Antibody-clone-RPA-T4-Monoclonal/61-0049-42">CD4 Monoclonal Antibody (RPA-T4), PE-eFluor</a> 610 (5 µL, Cat. No. 61-0049-42), <a data-uw-rm-kbnav="anohref"></a><a href="/antibody/product/CD4-Antibody-clone-SK3-SK-3-Monoclonal/H001T02Y03">CD4 Monoclonal Antibody (SK3 (SK-3))</a> NovaFluor Yellow 610 (5 µL, Cat. No. H001T02Y03), or <a data-uw-rm-kbnav="anohref"></a><a href="/antibody/product/CD19-Antibody-clone-HIB19-Monoclonal/H004T03B06">CD19 Monoclonal Antibody (HIB19)</a>, <a href="/antibody/product/CD19-Antibody-clone-HIB19-Monoclonal/H004T03B06">NovaFluor Blue 610-70S</a> (5 µL, Cat. No. H004T03B06). Beads were stained for 30 minutes in <a data-uw-rm-kbnav="anohref"></a><a href="/order/catalog/product/00-4222-26">eBioscience Flow Cytometry Staining Buffer</a> (Cat. No. 00-4222-26), washed, and then analyzed on an <a data-uw-rm-kbnav="anohref"></a><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/attune-nxt-flow-cytometer/models/cytpix.html">Attune CytPix Flow Cytometer </a>(4-laser configuration) (red arrows show spillover). Data shown here are raw fluorescent signal in the BL2 and YL2 channels for each fluorophore. Signal intensity is shown here (x axes) in the YL2 and BL2 channels, demonstrating the strong spillover in BL2 from PE-eFluor 610 dye. This spillover is mitigated with the use of NovaFluor dyes that occupy the same primary channels (BL2, YL2), allowing correct compensation and increased panel size.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-63368f6874" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-ctaitem m--btn-link m--center aem-GridColumn--offset--default--11 aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--1"><div class="cmp-ctaitem" id="ctaitem-00d317e394"> <a class="cmp-ctaitem__anchor" href="#top"> <span class="cmp-ctaitem__text">Top</span> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer mt-l2 m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-659f834657"> <div class="cmp-sectiontitle"> <h2 id="expansion" class="cmp-sectiontitle__text" data-anchor-nav-title="Designed for Panel Expansion ">Designed for panel expansion </h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-5b1b11679d" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-255280202" class="cmp-text"> <p><a id="expansion"></a>NovaFluor dyes’ enhanced spectral characteristics make them excellent candidates for panel expansion with minimal impacts to spillover and consequent spreading error.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-e1ab87c0a9" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-accordion aem-GridColumn aem-GridColumn--default--12"><div id="accordion-6d109e2ca2" class="cmp-accordion" data-cmp-is="accordion"> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-6d109e2ca2-item-44109d4f87" data-cmp-expanded> <h2 class="cmp-accordion__header"> <button id="accordion-6d109e2ca2-item-44109d4f87-button" class="cmp-accordion__button cmp-accordion__button--expanded" aria-controls="accordion-6d109e2ca2-item-44109d4f87-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">See how to expand panels </span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-6d109e2ca2-item-44109d4f87-panel" class="cmp-accordion__panel cmp-accordion__panel--expanded" role="region" aria-labelledby="accordion-6d109e2ca2-item-44109d4f87-button"><div class="cmp-p-container"> <div id="container-44109d4f87" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-1f8f05754b" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1409399350" class="cmp-text"> <p>Unlock the full power of your instrument through the enhanced spectral properties of NovaFluor dyes. Narrow excitation spectra enable broader utilization of the blue and yellow-green lasers on many flow cytometers. In this example, we see the typical channel distribution of a 12-color panel involving traditional dyes compared to a 14-color panel achieved by incorporating NovaFluor dyes.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-f42af7fc25" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2024/08/panel-expansion-design.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2024/08/panel-expansion-design.jpg" data-title="Table showing panel expansion design" id="image-1621084423" class="cmp-image hand-icon" itemscope itemtype="http://schema.org/ImageObject"> <div data-toggle="modal" data-target="#modal-image-1621084423" class="modal-toggle"> <img src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2024/08/panel-expansion-design.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Table showing panel expansion design"/> </div> </div>  <div class="cmp-image-modal modal" id="modal-image-1621084423" tabindex="-1" role="dialog" aria-labelledby="modal-label-for-image-1621084423"> <div class="cmp-image-modal__body modal-body"> <div class="cmp-image-modal__header modal-header no-border"> <button type="button" class="cmp-image-modal__close close" data-dismiss="modal" aria-label="Close"> <span class="cmp-image-modal__close__icon" aria-hidden="true">×</span> </button> </div>  <img loading="lazy" src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2024/08/panel-expansion-design.jpg" class="cmp-image-modal__body__image" itemprop="contentUrl" data-cmp-hook-image="image" alt="Table showing panel expansion design"/> </div> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-1748501537" class="cmp-text"> <p><strong>Table 1. Design of two <strong>multicolor flow cytometry panels showing panel expansion with NovaFluor dyes.</strong></strong> Panel A is 12-conjugate panel using traditional conjugates and Panel B is a 14-conjugate panel employing NovaFluor conjugates. Top rows indicate detectors and filter bandwidths, which are grouped by excitation source in order of increasing wavelength. Surface antigens are reported with conjugated fluorophores indicated. Exchanges in fluorophores are highlighted by color, while unoccupied detectors are indicated by “Open”. For example, CD3 PerCP-Cyanine5.5 used in Panel 1 is replaced by CD3 NovaFluor Blue 610-70S in Panel 2.<br /> </p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-a68b2f10fe" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/panel-expansion-data-r.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/panel-expansion-data-r.jpg" data-title=": Graph showing panel expansion" id="image-379171777" class="cmp-image hand-icon" itemscope itemtype="http://schema.org/ImageObject"> <div data-toggle="modal" data-target="#modal-image-379171777" class="modal-toggle"> <img src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/panel-expansion-data-r.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt=": Graph showing panel expansion"/> </div> </div>  <div class="cmp-image-modal modal" id="modal-image-379171777" tabindex="-1" role="dialog" aria-labelledby="modal-label-for-image-379171777"> <div class="cmp-image-modal__body modal-body"> <div class="cmp-image-modal__header modal-header no-border"> <button type="button" class="cmp-image-modal__close close" data-dismiss="modal" aria-label="Close"> <span class="cmp-image-modal__close__icon" aria-hidden="true">×</span> </button> </div>  <img loading="lazy" src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/panel-expansion-data.png" class="cmp-image-modal__body__image" itemprop="contentUrl" data-cmp-hook-image="image" alt=": Graph showing panel expansion"/> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-e7c86c6f83" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1448186928" class="cmp-text"> <p><b>Figure 4. Data from two multicolor flow cytometry panels showing panel expansion with NovaFluor dyes.</b> Comparison of flow cytometry plots as shown in Table 1 with the 12-conjugate panel (A) and 14-conjugate panel (B). All plots were gated on singlet, then viability, and finally lymphocytes. In addition, specific plots were sub-gated on populations as indicated above. Note arrows indicating areas of increased spread for Panel 1 vs Panel 2. Human peripheral blood mononuclear cells were stained <a data-uw-rm-kbnav="anohref"></a><a href="/order/catalog/product/65-0866-18" data-uw-rm-brl="false">Fixable Viability Dye eFluor 506</a> (Cat. No. 65-0866-18) then blocked with <a href="/antibody/product/CD19-Antibody-clone-HIB19-Monoclonal/48-0199-42" data-uw-rm-brl="false">Fc Receptor Binding Inhibitor Polyclonal Antibody</a> (Cat. No. 14-9161-73) and finally stained with the antibody conjugates indicated in Table 1 in the presence of <a href="/order/catalog/product/00-4409-75" data-uw-rm-brl="false"></a><a data-uw-rm-kbnav="anohref"></a><a href="/order/catalog/product/B001T06F01" data-uw-rm-brl="false">CellBlox Blocking Buffer</a> (Cat. No. B001T06F01) and <a data-uw-rm-kbnav="anohref"></a><a href="/order/catalog/product/00-4409-75" data-uw-rm-brl="false">Brilliant Stain Buffer </a>(Cat. No. 00-4409-75).</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-42fbce0412" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-ctaitem m--btn-link m--center aem-GridColumn--offset--default--11 aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--1"><div class="cmp-ctaitem" id="ctaitem-2c3dd2d77f"> <a class="cmp-ctaitem__anchor" href="#top"> <span class="cmp-ctaitem__text">Top</span> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer mt-l2 m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-8f379a61c0"> <div class="cmp-sectiontitle"> <h2 id="cellblox" class="cmp-sectiontitle__text" data-anchor-nav-title="Invitrogen CellBlox Plus Blocking Buffer ">Invitrogen CellBlox Plus Blocking Buffer </h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-84ab623da3" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-408648345" class="cmp-text"> <p><a id="cellblox"></a><a href="/order/catalog/product/C001T06F01" target="_self">Invitrogen CellBlox Plus Blocking Buffer</a> is formulated for enhanced blocking of nonspecific binding of NovaFluor antibody conjugates to cells. It provides exceptional blocking of nonspecific interactions when compared to CellBlox Blocking Buffer. Use of CellBlox Blocking Plus Buffer requires minimal change to most flow cytometry staining protocols.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-17a98bba19" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-accordion aem-GridColumn aem-GridColumn--default--12"><div id="accordion-1289504531" class="cmp-accordion" data-cmp-is="accordion"> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-1289504531-item-83cec4c9be"> <h2 class="cmp-accordion__header"> <button id="accordion-1289504531-item-83cec4c9be-button" class="cmp-accordion__button" aria-controls="accordion-1289504531-item-83cec4c9be-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">See benefits of CellBlox Plus Blocking Buffer </span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-1289504531-item-83cec4c9be-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" role="region" aria-labelledby="accordion-1289504531-item-83cec4c9be-button"><div class="cmp-p-container"> <div id="container-83cec4c9be" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-987689e884" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1750780539" class="cmp-text"> <p>Always use CellBlox Blocking Plus Buffer with NovaFluor dyes when labeling cells for best background reduction</p> <p> </p> <p>See how to use CellBlox Plus Blocking Buffer in the <a href="/sg/en/home/references/protocols/cell-and-tissue-analysis/protocols/staining-cell-surface-targets-flow-cytometry.html" target="_self">Cell Surface Staining Protocol</a>.</p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-c8c1b6a063" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/blocking-ability-of-cellblox-pl-r.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/blocking-ability-of-cellblox-pl-r.jpg" data-title="Blocking ability of CellBlox Plus Blocking buffer versus CellBlox Buffer" id="image-636224542" class="cmp-image hand-icon" itemscope itemtype="http://schema.org/ImageObject"> <div data-toggle="modal" data-target="#modal-image-636224542" class="modal-toggle"> <img src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/blocking-ability-of-cellblox-pl-r.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Peripheral blood mononuclear cells (PBMCs) were either unblocked (A), blocked with CellBlox Blocking Buffer (B001T06F01) (B), or CellBlox Plus Blocking Buffer (C001T06F01) (C). Cells were then stained with either CD4 monoclonal antibody, NovaFluor Yellow 755 (top) or CD4 monoclonal antibody, NovaFluor Red 710 (bottom). All cells were co-stained with CD19 monoclonal antibody, eFluor 450 (48-0199-42). Two-dimensional pseudocolor plots of CD4 vs CD19 show that the CellBlox Plus Blocking Buffer reduces non-specific binding compared to staining without CellBlox Plus Blocking Buffer and with CellBlox Blocking Buffer. Data were acquired on a 5-laser Cytek Aurora Flow Cytometer." title="Blocking ability of CellBlox Plus Blocking buffer versus CellBlox Buffer"/> </div> <meta itemprop="caption" content="Blocking ability of CellBlox Plus Blocking buffer versus CellBlox Buffer"/> </div>  <div class="cmp-image-modal modal" id="modal-image-636224542" tabindex="-1" role="dialog" aria-labelledby="modal-label-for-image-636224542"> <div class="cmp-image-modal__body modal-body"> <div class="cmp-image-modal__header modal-header no-border"> <button type="button" class="cmp-image-modal__close close" data-dismiss="modal" aria-label="Close"> <span class="cmp-image-modal__close__icon" aria-hidden="true">×</span> </button> </div>  <img loading="lazy" src="/content/dam/LifeTech/global/life-sciences/images/blocking--ability-of-cellblox-plus-1050x770.jpg" class="cmp-image-modal__body__image" itemprop="contentUrl" data-cmp-hook-image="image" alt="Peripheral blood mononuclear cells (PBMCs) were either unblocked (A), blocked with CellBlox Blocking Buffer (B001T06F01) (B), or CellBlox Plus Blocking Buffer (C001T06F01) (C). Cells were then stained with either CD4 monoclonal antibody, NovaFluor Yellow 755 (top) or CD4 monoclonal antibody, NovaFluor Red 710 (bottom). All cells were co-stained with CD19 monoclonal antibody, eFluor 450 (48-0199-42). Two-dimensional pseudocolor plots of CD4 vs CD19 show that the CellBlox Plus Blocking Buffer reduces non-specific binding compared to staining without CellBlox Plus Blocking Buffer and with CellBlox Blocking Buffer. Data were acquired on a 5-laser Cytek Aurora Flow Cytometer." title="Blocking ability of CellBlox Plus Blocking buffer versus CellBlox Buffer"/> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-b5f6fdf7c8" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-346944668" class="cmp-text"> <p><span class="muted"><strong>Figure 5. CellBlox Plus Blocking buffer offers improved blocking compared to CellBlox Buffer. </strong>Peripheral blood mononuclear cells (PBMCs) were either unblocked (A), blocked with <a href="/order/catalog/product/B001T06F01" data-uw-rm-brl="false">CellBlox Blocking Buffer</a> (B001T06F01) (B), or <a href="/order/catalog/product/C001T06F01" data-uw-rm-brl="false">CellBlox Plus Blocking Buffer</a> (C001T06F01) (C). Cells were then stained with either CD4 monoclonal antibody, NovaFluor Yellow 755 (top) or CD4 monoclonal antibody, NovaFluor Red 710 (bottom). All cells were co-stained with <a href="/antibody/product/CD19-Antibody-clone-HIB19-Monoclonal/48-0199-42" data-uw-rm-brl="false">CD19 Monoclonal Antibody (HIB19), eFluor 450</a> (48-0199-42). Two-dimensional pseudocolor plots of CD4 vs CD19 show that the CellBlox Plus Blocking Buffer reduces non-specific binding compared to staining without CellBlox Plus Blocking Buffer and with CellBlox Blocking Buffer. Data were acquired on a 5-laser Cytek Aurora Flow Cytometer.</span></p> <p> </p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-618c679630" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/improved-non-specific-blocking-1-r.jpg" data-asset="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/improved-non-specific-blocking-1-r.jpg" data-title="Graph showing reduction in background" id="image-1307730207" class="cmp-image hand-icon" itemscope itemtype="http://schema.org/ImageObject"> <div data-toggle="modal" data-target="#modal-image-1307730207" class="modal-toggle"> <img src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2022/11/improved-non-specific-blocking-1-r.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph showing reduction in background"/> </div> </div>  <div class="cmp-image-modal modal" id="modal-image-1307730207" tabindex="-1" role="dialog" aria-labelledby="modal-label-for-image-1307730207"> <div class="cmp-image-modal__body modal-body"> <div class="cmp-image-modal__header modal-header no-border"> <button type="button" class="cmp-image-modal__close close" data-dismiss="modal" aria-label="Close"> <span class="cmp-image-modal__close__icon" aria-hidden="true">×</span> </button> </div>  <img loading="lazy" src="/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2023/07/improved-non-specific-blocking-1050x760.jpg" class="cmp-image-modal__body__image" itemprop="contentUrl" data-cmp-hook-image="image" alt="Graph showing reduction in background"/> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-88c155b6f9" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-599806699" class="cmp-text"> <p><span class="muted"><b><br /> Figure 6. CD3 labeling, combined with CellBlox Plus Blocking Buffer, is shown to reduce background in lymphocytes and non-specific labeling of monocytes and macrophages compared with labeling without CellBlox Plus Blocking Buffer.</b> Peripheral blood mononuclear cells (PBMCs) were either left unstained (black), stained with <a href="/antibody/product/CD3-Antibody-clone-UCHT1-Monoclonal/H002T03B08" data-uw-rm-brl="false">CD3 Monoclonal Antibody (UCHT1), NovaFluor Blue 660 120S</a> conjugate (H002T03B08) with (red), and without (blue) the addition of <a href="/order/catalog/product/C001T06F01" data-uw-rm-brl="false">CellBlox Plus Blocking Buffer</a> (C001T06F01). (A) Forward Scatter vs. Side Scatter pseudocolor plot shows lymphocyte and monocyte gates. Histogram overlay plots show the expression of CD3 within the (B) lymphocyte and (C) monocyte gates. Data were acquired in the B7 channel on a 5-laser Cytek Aurora.</span></p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-53c03391ac" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-ctaitem m--btn-link m--center aem-GridColumn--offset--default--11 aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--1"><div class="cmp-ctaitem" id="ctaitem-b5680765e9"> <a class="cmp-ctaitem__anchor" href="#top"> <span class="cmp-ctaitem__text">Top</span> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer mt-l2 m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-c08d30786e"> <div class="cmp-sectiontitle"> <h2 id="new" class="cmp-sectiontitle__text" data-anchor-nav-title="New discoveries and insights">New discoveries and insights</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-6cc0abab3c" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1868181243" class="cmp-text"> <p>Discover the latest updates, data insights, and educational content from NovaFlour. Stay informed, explore new experiments, and enhance your scientific journey with our helpful tips and stories. </p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-ed5e2a3ff8" class="cmp-container"> <div class="cmp-p-accordion"><div id="accordion-23eecd0075" class="cmp-accordion" data-cmp-is="accordion"> <div class="cmp-accordion__item" data-cmp-hook-accordion="item" id="accordion-23eecd0075-item-fbe5b0f863"> <h2 class="cmp-accordion__header"> <button id="accordion-23eecd0075-item-fbe5b0f863-button" class="cmp-accordion__button" aria-controls="accordion-23eecd0075-item-fbe5b0f863-panel" data-cmp-hook-accordion="button"> <span class="cmp-accordion__title">Is Brighter Always Better in Flow Cytometry?</span> <span class="cmp-accordion__icon"></span> </button> </h2> <div data-cmp-hook-accordion="panel" id="accordion-23eecd0075-item-fbe5b0f863-panel" class="cmp-accordion__panel cmp-accordion__panel--hidden" 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