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Successfully transfected cell types include:<br/><br/>Fibroblast (3T3, COS-7)<br/>Myoblast (C2C12, L6 CRL-1458)<br/>Hepatocyte (HepG2, HuH7)<br/>Erythroleukemic cell (K562)<br/>Breast carcinoma (MCF7, Hs578T)<br/>Prostate carcinoma (LNCap)<br/>Lung carcinoma (A549, NCI-H460)<br/>Osteosarcoma (U-2 OS, Saos-2)<br/>Colon carcinoma (Caco2, SW480)<br/>Pancreatic carcinoma (PANC-1)<br/>Skin melanoma (SK-MEL-28)<br/><br/><a href='http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/transfection-selection/lipofectamine-3000.html#enhance'>View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 ></a><br/><br/><b>Transfect Common Cells Efficiently and Cost-Effectively</b><br/>For common cell types, Lipofectamine™ 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. 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Successfully transfected cell types include:<br/><br/>Fibroblast (3T3, COS-7)<br/>Myoblast (C2C12, L6 CRL-1458)<br/>Hepatocyte (HepG2, HuH7)<br/>Erythroleukemic cell (K562)<br/>Breast carcinoma (MCF7, Hs578T)<br/>Prostate carcinoma (LNCap)<br/>Lung carcinoma (A549, NCI-H460)<br/>Osteosarcoma (U-2 OS, Saos-2)<br/>Colon carcinoma (Caco2, SW480)<br/>Pancreatic carcinoma (PANC-1)<br/>Skin melanoma (SK-MEL-28)<br/><br/><a href='http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/transfection-selection/lipofectamine-3000.html#enhance'>View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 ></a><br/><br/><b>Transfect Common Cells Efficiently and Cost-Effectively</b><br/>For common cell types, Lipofectamine™ 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. 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The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).<br/><br/><b>Achieve Superior Transfection Efficiency While Maintaining Low Toxicity</b><br/> Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.<br/><br/><b>Enable High Transfection Efficiency in Novel Genome Editing Applications</b><br/>Lipofectamine™ 3000 reagent is suitable for novel genome editing applications. 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Successfully transfected cell types include:<br/><br/>Fibroblast (3T3, COS-7)<br/>Myoblast (C2C12, L6 CRL-1458)<br/>Hepatocyte (HepG2, HuH7)<br/>Erythroleukemic cell (K562)<br/>Breast carcinoma (MCF7, Hs578T)<br/>Prostate carcinoma (LNCap)<br/>Lung carcinoma (A549, NCI-H460)<br/>Osteosarcoma (U-2 OS, Saos-2)<br/>Colon carcinoma (Caco2, SW480)<br/>Pancreatic carcinoma (PANC-1)<br/>Skin melanoma (SK-MEL-28)<br/><br/><a href='http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/transfection-selection/lipofectamine-3000.html#enhance'>View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 ></a><br/><br/><b>Transfect Common Cells Efficiently and Cost-Effectively</b><br/>For common cell types, Lipofectamine™ 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).<br/><br/><b>Achieve Superior Transfection Efficiency While Maintaining Low Toxicity</b><br/> Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.<br/><br/><b>Enable High Transfection Efficiency in Novel Genome Editing Applications</b><br/>Lipofectamine™ 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.","productLongDescription":"Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. 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Successfully transfected cell types include:<br/><br/>Fibroblast (3T3, COS-7)<br/>Myoblast (C2C12, L6 CRL-1458)<br/>Hepatocyte (HepG2, HuH7)<br/>Erythroleukemic cell (K562)<br/>Breast carcinoma (MCF7, Hs578T)<br/>Prostate carcinoma (LNCap)<br/>Lung carcinoma (A549, NCI-H460)<br/>Osteosarcoma (U-2 OS, Saos-2)<br/>Colon carcinoma (Caco2, SW480)<br/>Pancreatic carcinoma (PANC-1)<br/>Skin melanoma (SK-MEL-28)<br/><br/><a href='http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/transfection-selection/lipofectamine-3000.html#enhance'>View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 ></a><br/><br/><b>Transfect Common Cells Efficiently and Cost-Effectively</b><br/>For common cell types, Lipofectamine™ 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).<br/><br/><b>Achieve Superior Transfection Efficiency While Maintaining Low Toxicity</b><br/> Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.<br/><br/><b>Enable High Transfection Efficiency in Novel Genome Editing Applications</b><br/>Lipofectamine™ 3000 reagent is suitable for novel genome editing applications. 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as a Method * define function as object methods * @type {Object} */ window.digitalData = { user: {}, page: {}, merchandising: {}, getQueryParam : function (param) { var index, key, value, hash; var hashes = window.location.href.slice(window.location.href.indexOf('?') + 1).split('&'); for (index = 0; index < hashes.length; index++) { hash = hashes[index].split('='); key = hash[0]; value = hash[1]; if (key.toUpperCase() === param.toUpperCase()) return value; } return ''; }, getValOnce: function (value, cookieName, expiry, timeUnit) { var currentDate = new Date; var currentCookieValue; // timeUnit of 'm' is minutes represented in milliseconds // thus 86400000 = 24 hours = 1 day var interval = timeUnit == 'm' ? 60000 : 86400000; cookieName = cookieName ? cookieName : 'digitaldata_gvo'; value = value ? value : ''; expiry = expiry ? expiry : 0; currentCookieValue = this.getCookie(cookieName); if (value) { currentDate.setTime(currentDate.getTime() + expiry * interval); this.setCookie(cookieName, value, expiry == 0 ? 0 : currentDate); } return value == currentCookieValue ? '' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); var refReg = document.referrer; var referrerDomain = refReg.match(/:\/\/([^/]+)\//); if (referrerDomain !== null && referrerDomain[1].indexOf(masterDomain) == -1) { this.merchandising.referrer = referrerDomain; } } document.addEventListener('registerMerchandisingData', function (event) { for (var key in event.detail) { console.log("Listener data copied", key, event.detail[key] ); digitalData.merchandising[key] = event.detail[key]; } // Map some analytics attributes to merchandising session attributes digitalData.merchandising.sessionId = digitalData.merchandising.analyticsVisitorID; digitalData.merchandising.s_vi_low = digitalData.merchandising.analyticsVisitorIDLow; digitalData.merchandising.s_vi_high = digitalData.merchandising.analyticsVisitorIDHigh; console.log("digitalData: Captured registerMerchandisingData event"); digitalData.merchandisingDataInitted = true; // Call emailCampaignRecord if the digitalDataAnalyticsListener object is loaded if (typeof digitalDataAnalyticsListener != "undefined") { console.log("digitalData: Calling email campaign record"); digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. Please try again later.", "MINICART_ERROR_2" :"Please enter a quantity in the range of 1 to 999.", "MINICART_ERROR_3" :"Could not add item(s) to cart.", "MINICART_ERROR_4" :"Could not remove item from cart.", "MINICART_ERROR_5" :"Could not create/restore cart.", "MINICART_ERROR_6" :"Unable to retrieve cart details. Please try again later.", "MINICART_MESSAGE_1" :"Adding item(s) to cart...", "MINICART_MESSAGE_2" :"Removing item(s) from cart...", "MINICART_CART_NAME_LABEL_1" :"Recently added to the", "MINICART_NOTE_1" : "Your total price and product details can be viewed in the cart.", MINICART_SUMMARY : "Cart summary", MINICART_PRICE : "Price", MINICART_PRODUCT : "Product", LIST_PRICE : "Price", YOUR_PRICE : "Your price", WEB_PRICE: "Online offer", PRICE_ENDS: "ends", SIGN_IN: "Sign in", //New mini cart CATALOG_NUMBER: "Catalog number:", CONTACT_US: "Contact us", RFQ: "Request quote", LEARN_WHERE_TO_BUY: "Learn where to buy", CURRENCY: "USD", //End new mini cart "CHANGE_CART" :"Change cart", "VIEW_ALL" :"View All", "ITEM" :"item", "ITEMS" :"items", "CLOSE" :"Close", "SUBTOTAL" :"Subtotal", "TOTAL" :"Total", "MANAGE_CART" :"Manage Cart", "FIELD" :"field", "GLOBAL_ERROR_1" :"Please choose one or both of the options for", "GLOBAL_ERROR_2" :"is not a valid e-mail address.", "GLOBAL_ERROR_3" :"Unsupported character(s) were found in field", "GLOBAL_ERROR_4" :"and will be replaced with plain text equivalents (where available). Please review the changes and try again.", "GLOBAL_ERROR_5" :"Please fill in the", "GLOBAL_ERROR_6" :"Quantities must be whole numbers greater than or equal to zero.", "GLOBAL_ERROR_7" :"is either not a number or a number less than zero, please provide a numerical quantity", "GLOBAL_ERROR_8" :"Reserved character | not allowed. Please remove and try again.", "required" : "One or more required fields are missing", "requireOneOfMany" : "At least one field in the group must be filled out", "password" : "Password does not conform to the password policy", "passwordConfirm" : "Password and Confirm Password do not match", "emailConfirm" : "Email and Confirm Email do not match", "secretConfirm" : "Secret Answer and Confirm Secret Answer do not match", "alphanumeric" : "Value must be alphanumeric", "phone" : "Phone number or Fax number provided were non-numeric values", "email" : "Email address provided is in an invalid format", "date" : "Date is in an invalid format (ex. MM/DD/YYYY)", "expirationDate" : "Expiration date provided is invalid", "currency" : "Currency is in an invalid format", "creditCard" : "Credit card number is invalid", "quantity" : "Quantity must in the range of 1 - 999", "acceptance" : "The terms and conditions must be accepted to continue", "checkboxes" : "At least one item must be selected", "twoOrMorecheckboxes" : "Merging carts requires multiple carts to be selected", "file" : "A file must be selected to upload", "NEW_USER" :"New user", "REGISTER_NOW" :"Register Now", "LOGIN_WIDGET_MESSAGE1" :"View your contract prices and real-time product availability.", "WELCOME" :"Welcome", "MY_ACCOUNT" :"My Account", "FAVORITES" :"Favorites", "SAVED_LISTS" :"Saved Lists", "ORDER_STATUS" :"Order Status", "LOGOUT" :"Logout", "APPROVALS" :"Approvals", "REMOVE" :"Remove", "QUANTITY" :"Qty", "EDIT" :"Edit", "SEARCH_BOX_TEXT" :"",//"Search by catalog number or keyword", "SELECT_LANGUAGE" :"Select a Language", "MY_MESSAGES" :"My Messages", "MESSAGE_ALERTS" :"Message Alerts", "B2B_NEW_WINDOW_ALERT" :"You are about to open a new browser window. \nClicking an external link on our site causes a new window to open. However, your shopping session will remain active in this window.Simply return here to continue shopping. \n Would you like to proceed?", "GREETING" :"", "SCMS_SHIP_TO_LAB" :"Ship To Lab", "SCMS_SHIP_TO_SC" :"Supply Center Order", "SCMS_SHIP_TO_SC_FOR_EU":"Supply Centre Order", "SUBMIT" :"Submit", "SUBMIT_ERROR_VALIDATE_EMAIL":"Sorry - we could not validate your email address. Please try again. 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Click on this icon to learn more." alignment="start" placement="right"><img src="static/images/green-icon.svg" alt="Green features"/></core-tooltip></span><div class="short-description"><span>Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application<span class="last-description"> outcomes</span></span> <span class="read-more">Read more</span></div></div><div class="pdp-promos"></div><div class="pdp-product-selector"><div class="pdp-product-selector__icons-options-container"><div class="pdp-product-selector__contact-us-popup"><span class="pdp-product-selector__contact-us-text">Have Questions?</span><div class="pdp-contact-us"></div></div><div class="pdp-product-selector__icons"><span class="pdp-product-selector__text">Change view</span><core-segmentedbuttons><core-segmentedbuttons-item nopadding="true" selected="false" slot="segment"><img slot="content" src="data:image/svg+xml;base64,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" alt="buttonView"/></core-segmentedbuttons-item><core-segmentedbuttons-item nopadding="true" selected="true" slot="segment"><img slot="content" src="data:image/svg+xml;base64,PHN2ZyB3aWR0aD0iNDgiIGhlaWdodD0iMzIiIHZpZXdCb3g9IjAgMCA0OCAzMiIgZmlsbD0ibm9uZSIgeG1sbnM9Imh0dHA6Ly93d3cudzMub3JnLzIwMDAvc3ZnIj4KPHJlY3Qgd2lkdGg9IjQ4IiBoZWlnaHQ9IjMyIiByeD0iNCIgZmlsbD0iIzAwNzFEMCIvPgo8cGF0aCBmaWxsLXJ1bGU9ImV2ZW5vZGQiIGNsaXAtcnVsZT0iZXZlbm9kZCIgZD0iTTE0IDZIMzRWMjZIMTRWNlpNMTYgMTEuOTcxSDMyVjhIMTZWMTEuOTcxWk0zMiAxNy45ODZIMTZWMTQuMDE0SDMyVjE3Ljk4NlpNMzIgMjRIMTZWMjAuMDI4SDMyVjI0WiIgZmlsbD0id2hpdGUiLz4KPC9zdmc+Cg==" alt="tableView"/></core-segmentedbuttons-item></core-segmentedbuttons></div></div><div class="pdp-table-wrapper"><div class="pdp-table-product-selector"><table><thead><tr><th class="">Catalog Number</th><th>Quantity</th></tr></thead><tbody><tr class="pdp-table-product-selector__active-row"><td class=""><span class="pdp-table-product-selector__catalog-number">L3000015</span></td><td>1.5 mL</td></tr><tr class=""><td class=""><span class="pdp-table-product-selector__catalog-number">L3000001</span></td><td>0.1 mL</td></tr><tr class=""><td class=""><span class="pdp-table-product-selector__catalog-number">L3000008</span></td><td>0.75 mL</td></tr><tr class=""><td class=""><span class="pdp-table-product-selector__catalog-number">L3000075</span></td><td>5 x 1.5 mL</td></tr><tr class=""><td class=""><span class="pdp-table-product-selector__catalog-number">L3000150</span></td><td>15 mL</td></tr></tbody></table></div><div class="pdp-options" style="bottom:0px;width:100%;visibility:hidden"> 5 Options </div></div></div></div><div class="pdp-commerce-box col-lg-3 col-md-12"><div class="commerce-box-inner"><div class="pdp-card"><div class="pdp-commerce-box-content" slot="content"><div class="pdp-commerce-box--first-row"><span class="pdp-commerce-box--first-row--catalog-number-wrapper"><span class="pdp-commerce-box--catalog-number">Catalog number </span><span class="pdp-commerce-box--catalog-number-value">L3000015</span></span><div data-testid="favorites-component"><span data-testid="favorites-icon" class="pdp-favorites-icon"><core-tooltip label="Your favorites are now located within Lists. Select to save this product to a list." alignment="end" placement="bottom"><span class="icon-24-rating-mono"></span></core-tooltip></span></div></div><div class="pdp-divider"></div><div class="pdp-price"><span class="pdp-price__price-label">Price (SGD)</span><span class="pdp-price__productSize">/ 1.5 mL</span><div><div class="pdp-legacy-price"><span class="pdp-legacy-price-label">Price:</span><span class="pdp-legacy-price-lp-value">1,281.00</span></div><div class="pdp-legacy-online-price"><span class="pdp-legacy-online-price-label">Online offer:</span><span class="pdp-legacy-online-price-value">1,089.00</span><span class="pdp-legacy-online-price-tooltip"><core-tooltip alignment="center" placement="bottom"><span slot="tooltip-content">Web orders only. Excludes Supply Centers.</span><span class="icon-16-info-mono"></span></core-tooltip></span><div class="pdp-legacy-online-price-end-date">(ends 01-Jan-2025)</div></div><div><span class="pdp-legacy-your-price-label">Your Price:</span><span class="pdp-legacy-your-price-sign-in"><span class="pdp-legacy-your-price-sign-in-label">Sign In</span><span class="pdp-legacy-your-price-tooltip"><core-tooltip alignment="center" placement="bottom"><span slot="tooltip-content">Sign in to see your account pricing. Need an account? Register with us today.</span><span class="icon-16-info-mono"></span></core-tooltip></span></span></div></div></div><div class="pdp-divider"></div><div class="pdp-commerce-box__wrapper"><div class="pdp-quantity"><core-quantityselector inputwidth="52px" value="1" max="9999"></core-quantityselector></div><div class="pdp-availability">-<div class="pdp-scms-tooltip"></div></div></div><div></div><div class="pdp-add-to-cart pdp_commerce_add_to_cart"><core-button type="button" variant="primary" size="medium" fullspace="true" data-parameters="doc_id, signal_type, app_id, element_clicked, product_qty, price" data-signal_type="add_to_cart" data-element_clicked="pdp-add-to-cart" data-app_id="tfcom-product-details-ui-v3" data-doc_id="L3000015" data-product_qty="1" data-price="1089 SGD"><span slot="content">Add to cart</span></core-button></div><div class="pdp-ordering-attributes"><div class="pdp-ordering-attribute"><div class="pdp-ordering-attribute__label">Quantity:</div><div class="pdp-ordering-attribute__value">1.5 mL</div></div></div></div></div><div class="pdp-recurring-order"></div><div class="pdp-custom-forms"></div></div></div><div class="pdp-add-to-cart-box-mobile" style="top:0px"><div class="pdp-cart-box"><div class="pdp-cart-box__price"><div class="pdp-price sticky"><span class="pdp-price__price-label">Price (SGD)</span><span class="pdp-price__productSize">/ 1.5 mL</span><div class="pdp-legacy-online-price"><span class="pdp-legacy-online-price-label">Online offer:</span><span class="pdp-legacy-online-price-value">1,089.00</span><span class="pdp-legacy-online-price-tooltip"><core-tooltip alignment="center" placement="bottom"><span slot="tooltip-content">Web orders only. Excludes Supply Centers.</span><span class="icon-16-info-mono"></span></core-tooltip></span><div class="pdp-legacy-online-price-end-date">(ends 01-Jan-2025)</div></div></div></div><div class="pdp-cart-box__action"><div class="pdp-cart-box__quantity-value"><div class="pdp-quantity"><core-quantityselector inputwidth="52px" value="1" max="9999"></core-quantityselector></div></div><div class="pdp-cart-box__add-to-cart-btn"><div class="pdp-add-to-cart"><core-button type="button" variant="primary" size="small" fullspace="true" data-parameters="doc_id, signal_type, app_id, element_clicked, product_qty, price" data-signal_type="add_to_cart" data-element_clicked="pdp-add-to-cart" data-app_id="tfcom-product-details-ui-v3" data-doc_id="L3000015" data-product_qty="1" data-price="1089 SGD"><span slot="content">Add to cart</span></core-button></div></div></div></div></div><div class="pdp-add-to-cart-desktop" style="top:0px"><div class="pdp-add-to-cart-sticky-desktop"><div class="pdp-title-catalog-number"><div class="pdp-title">Lipofectamine™ 3000 Transfection Reagent</div><div><span class="pdp-catalog-number"><span class="pdp-catalog-number-label">Catalog number</span>L3000015</span></div></div><div class="pdp-price-sticky"><div class="pdp-price sticky"><span class="pdp-price__price-label">Price (SGD)</span><span class="pdp-price__productSize">/ 1.5 mL</span><div class="pdp-legacy-online-price"><span class="pdp-legacy-online-price-label">Online offer:</span><span class="pdp-legacy-online-price-value">1,089.00</span><span class="pdp-legacy-online-price-tooltip"><core-tooltip alignment="center" placement="bottom"><span slot="tooltip-content">Web orders only. Excludes Supply Centers.</span><span class="icon-16-info-mono"></span></core-tooltip></span><div class="pdp-legacy-online-price-end-date">(ends 01-Jan-2025)</div></div></div></div><div class="pdp-quantity"><div class="pdp-quantity"><core-quantityselector inputwidth="52px" value="1" max="9999"></core-quantityselector></div></div><div class="pdp-availability">-</div><div class="pdp-cart"><div class="pdp-add-to-cart sticky"><core-button type="button" variant="primary" size="medium" fullspace="true" data-parameters="doc_id, signal_type, app_id, element_clicked, product_qty, price" data-signal_type="add_to_cart" data-element_clicked="pdp-add-to-cart" data-app_id="tfcom-product-details-ui-v3" data-doc_id="L3000015" data-product_qty="1" data-price="1089 SGD"><span slot="content">Add to cart</span></core-button></div></div></div></div></div></div><div class="pdp-details"><div class="pdp-nav" style="top:0px"><core-navigationbar variant="horizontal"><core-navigationbar-item slot="nav-item" selected="true" variant="horizontal" label="Product Overview" href="/" target="_top"></core-navigationbar-item><core-navigationbar-item slot="nav-item" selected="false" variant="horizontal" label="Documents" href="/" target="_top"></core-navigationbar-item></core-navigationbar></div><div class="pdp-nav-containers"><div class="pdp-nav-container pdp-product-overview row" id="productOverview"><div class="pdp-product-description col-md-12 col-lg-7 "><div class="pdp-product-description-inner" id="product-description">Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).<br/><br/> Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:<br/><br/> • Superior performance—our highest efficiency reagent for difficult-to-transfect cells<br/>• Improved cell viability—gentle on your cells, with low toxicity<br/>• Versatile—one reagent for DNA, RNA, and co-transfection<br/><br/> <b>Efficiently Transfect Difficult-to-Transfect Cells</b><br/> Lipofectamine™ 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:<br/><br/>Fibroblast (3T3, COS-7)<br/>Myoblast (C2C12, L6 CRL-1458)<br/>Hepatocyte (HepG2, HuH7)<br/>Erythroleukemic cell (K562)<br/>Breast carcinoma (MCF7, Hs578T)<br/>Prostate carcinoma (LNCap)<br/>Lung carcinoma (A549, NCI-H460)<br/>Osteosarcoma (U-2 OS, Saos-2)<br/>Colon carcinoma (Caco2, SW480)<br/>Pancreatic carcinoma (PANC-1)<br/>Skin melanoma (SK-MEL-28)<br/><br/><a href='http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/transfection-selection/lipofectamine-3000.html#enhance'>View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 ></a><br/><br/><b>Transfect Common Cells Efficiently and Cost-Effectively</b><br/>For common cell types, Lipofectamine™ 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).<br/><br/><b>Achieve Superior Transfection Efficiency While Maintaining Low Toxicity</b><br/> Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.<br/><br/><b>Enable High Transfection Efficiency in Novel Genome Editing Applications</b><br/>Lipofectamine™ 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.</div><div class="pdp-product-description-inner pdp-product-description-disclaimer">For Research Use Only. Not for use in diagnostic procedures.</div></div><div class="col-lg-5 col-md-12"><div class="pdp-specifications"><div class="pdp-card pdp-specifications-card nopadding"><div class="pdp-specifications__wrapper" slot="content"><div class="pdp-specifications__title"><span class="pdp-specifications__text">Specifications</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Classification</span><span class="pdp-specification-row__value">Animal Origin-Free</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Cell Type</span><span class="pdp-specification-row__value">Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Format</span><span class="pdp-specification-row__value">6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Sample Type</span><span class="pdp-specification-row__value">Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Transfection Technique</span><span class="pdp-specification-row__value">Lipid-based Transfection</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">For Use With (Application)</span><span class="pdp-specification-row__value">Transfection</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Green Features</span><span class="pdp-specification-row__value">Sustainable packaging</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Product Line</span><span class="pdp-specification-row__value">Lipofectamine™</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Product Type</span><span class="pdp-specification-row__value">Transfection Reagent</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Quantity</span><span class="pdp-specification-row__value">1.5 mL</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Serum Compatible</span><span class="pdp-specification-row__value">Yes</span></div><div class="pdp-specification-row"><span class="pdp-specification-row__name">Unit Size</span><span class="pdp-specification-row__value">1.5 mL</span></div></div></div></div><div class="contents-storage"><div class="pdp-contents-and-storage"><core-card variant="floating" nopadding="true"><div class="pdp-contents-and-storage__wrapper" slot="content"><div class="pdp-contents-and-storage__title"><span class="pdp-contents-and-storage__text">Contents & Storage</span></div><div class="pdp-contents-and-storage__desc">Contains one vial of Lipofectamine™ 3000 Reagent (1.5 mL) and one vial of P3000TM Enhancer Reagent (1.5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. Store at 2–8°C. Do not freeze.</div></div></core-card></div></div><div class="offers-wrapper"></div></div></div></div></div></div></div> <script src="/store/v3/products/static/main.cc5dd2ca25cc5de0116b.js" async></script> <style> .no-logo{ min-height: 80px; } </style> <script> window.showGlobalFooter = function() { var footer = document.getElementById("tf-global-footer"); if (footer) { footer.style.display = 'block'; } } </script> <footer id="tf-global-footer" style="display: none;"> <div class="globalFooter" data-orig="tfpt-header-footer" data-app-env="prod" data-site="tf" data-hostname="www.thermofisher.com" data-x-host="www.thermofisher.com" data-env="prod" data-hash="468bd07150dbbf" data-version="4.03.29" data-analytics-section="header" data-aem-syndication-info="(sg:en-sg:anonymous:https-tf)" data-aem-syndication-timestamp="globalHeader: Tue, 26 Nov 2024 23:05:21 GMT" data-optional-selectors="inlineCss,lazyLoad,globalRoute">           <script type="text/javascript"> window.performance && 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