A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

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Thus, use of a DUMP channel may become crucia …"><meta name="keywords" content="pmid:21745365, PMC3148571, doi:10.1186/1479-5876-9-108, Sebastian Attig, Leah Price, CollabAuthor(name='CRI-CIC Assay Working Group', investigators=[], affs=[]), Biomarkers / metabolism*, CD8-Positive T-Lymphocytes / cytology, CD8-Positive T-Lymphocytes / immunology, Cell Survival, Coloring Agents / metabolism, Computational Biology, HLA Antigens / metabolism*, Humans, Lymphocyte Count, Peptides / metabolism*, Protein Binding, Protein Multimerization*, Reproducibility of Results, Staining and Labeling / methods*, Subcellular Fractions / metabolism, PubMed Abstract, NIH, NLM, NCBI, National Institutes of Health, National Center for Biotechnology Information, National Library of Medicine, MEDLINE"><meta name="robots" content="index,nofollow,noarchive"><meta property="og:title" content="A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols - PubMed"><meta property="og:url" content="https://web.archive.org/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/21745365/"><meta property="og:description" content="We conclude that appropriate use of a DUMP channel can significantly reduce background staining across a large fraction of protocols and improve the ability to accurately detect and quantify the frequency of antigen-specific T cells by multimer reagents. 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name="email-subject" value="1 selected item: 21745365"/> <input type="hidden" name="email-subject-hash" value="3dfe590b4a81f0f02cae267426e971c777ed60610a1cad8f17e53e768028e15b"/> </div> <div class="action-panel-control-wrap"> <label for="email-to" class="action-panel-label required-field-asterisk"> To: </label> <input type="email" aria-label="Recipient Email Address" placeholder="email@example.com" id="email-to" class="email-to" pattern="^[a-zA-Z0-9_.+-]+@[a-zA-Z0-9-]+\.[a-zA-Z0-9-.]+$" maxlength="100" required> </div> <div class="action-panel-control-wrap"> <label for="email-from" class="action-panel-label"> From: </label> <input type="email" aria-label="Sender Email Address" placeholder="email@example.com" id="email-from" class="email-from" pattern="^[a-zA-Z0-9_.+-]+@[a-zA-Z0-9-]+\.[a-zA-Z0-9-.]+$" maxlength="256"> </div> <div class="action-panel-control-wrap"> <label for="email-citation-format" class="action-panel-label"> Format: </label> <select id="email-citation-format" name="citation-format" class="action-panel-selector email-citation-format"> <option selected="selected" value="summary">Summary</option> <option value="summary-text">Summary (text)</option> <option value="abstract">Abstract</option> <option value="abstract-text">Abstract (text)</option> </select> </div> <div class="include-supplemental-container"> <input type="checkbox" aria-label="Include MeSH and other data" name="include-supplemental" id="email-include-supplemental" class="email-include-supplemental"> <label for="email-include-supplemental" class="email-include-supplemental-label">MeSH and other data</label> </div> <div class="form-field recaptcha "> <div class="g-recaptcha" id="id-recaptcha" data-sitekey="6LfsWHMdAAAAAClKbtOpjQ2pMjgsGxvv7NdZW9uI"></div> </div> <div id="captcha-error-message" class="usa-input-error-message captcha-validation-message" role="alert"></div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Sending..." data-ga-category="save_share" data-ga-action="email" data-ga-label="send"> Send email </button> <button class="action-panel-cancel" aria-label="Close 'Email citations' panel" ref="linksrc=close_email_panel" aria-controls="email-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="email" data-ga-label="cancel"> Cancel </button> </div> <input type="hidden" name="email-search-details" value=""/> <input type="hidden" name="email-search-details-hash" value="0e42663a6c3bd85498fcb88798998fed7bfdc45d457db35281e41afe13cc0524"/> </form> </div> </div> <div id="collections-action-panel" class="collections-action-panel action-panel in-progress-dots-panel" aria-hidden="true" data-collections-open-panel-enabled="false" data-collections-open-panel-url-hash="#open-collections-panel"> <div class="inner-wrap"> <h3 class="action-panel-heading"> Add to Collections </h3> <form id="collections-action-panel-form" class="collections-action-panel-form action-panel-content action-form action-panel-smaller-selectors" data-existing-collections-url="/list-existing-collections/" data-add-to-existing-collection-url="/add-to-existing-collection/" data-create-and-add-to-new-collection-url="/create-and-add-to-new-collection/" data-get-article-ids-by-search-url="/get-article-ids-by-search/" data-myncbi-max-collection-name-length="100" data-add-to-collection-max-amount="1000" data-collections-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/"> <input type="hidden" name="csrfmiddlewaretoken" value="Hr3Bicli5ECCEJn2kRckXawX6RP7QNpb1AV0DWVcwN3h8T7TLCI5zoyikWOKxspU"> <div class="choice-group" role="radiogroup"> <ul class="radio-group-items"> <li> <input type="radio" id="collections-action-panel-new" class="collections-new" name="collections" value="new" data-ga-category="save_share" data-ga-action="collections" data-ga-label="collections_radio_new"> <label for="collections-action-panel-new">Create a new collection</label> </li> <li> <input type="radio" id="collections-action-panel-existing" class="collections-existing" name="collections" value="existing" checked="true" data-ga-category="save_share" data-ga-action="collections" data-ga-label="collections_radio_existing"> <label for="collections-action-panel-existing">Add to an existing collection</label> </li> </ul> </div> <div class="controls-wrapper"> <div class="action-panel-control-wrap new-collections-controls"> <label for="collections-action-panel-add-to-new" class="action-panel-label required-field-asterisk"> Name your collection: </label> <input type="text" name="add-to-new-collection" id="collections-action-panel-add-to-new" class="collections-action-add-to-new" pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/" maxlength="100" data-ga-category="save_share" data-ga-action="create_collection" data-ga-label="non_favorties_collection"> <div class="collections-new-name-too-long usa-input-error-message selection-validation-message"> Name must be less than 100 characters </div> </div> <div class="action-panel-control-wrap existing-collections-controls"> <label for="collections-action-panel-add-to-existing" class="action-panel-label"> Choose a collection: </label> <select id="collections-action-panel-add-to-existing" class="action-panel-selector collections-action-add-to-existing" data-ga-category="save_share" data-ga-action="select_collection" data-ga-label="($('#collections-action-add-to-existing').val() === 'Favorites') ? 'Favorites' : 'non_favorites_collection'"> </select> <div class="collections-retry-load-on-error usa-input-error-message selection-validation-message"> Unable to load your collection due to an error<br> <a href="#">Please try again</a> </div> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Adding..." data-pinger-ignore data-ga-category="save_share" data-ga-action="collections" data-ga-label="add"> Add </button> <button class="action-panel-cancel" aria-label="Close 'Add to Collections' panel" ref="linksrc=close_collections_panel" aria-controls="collections-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="collections" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="bibliography-action-panel" class="bibliography-action-panel action-panel in-progress-dots-panel" aria-hidden="true" data-bibliography-open-panel-enabled="false" data-bibliography-open-panel-url-hash="#open-bibliography-panel"> <div class="inner-wrap"> <h3 class="action-panel-heading"> Add to My Bibliography </h3> <form id="bibliography-action-panel-form" class="bibliography-action-panel-form action-panel-content action-form action-panel-smaller-selectors" data-add-to-bibliography-max-amount="100" data-add-to-bibliography-batch-size="10" data-bibliography-delegates-url="/list-bibliography-delegates/" data-add-to-bibliography-url="/add-to-bibliography/" data-get-article-ids-by-search-url="/get-article-ids-by-search/" data-mybib-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/mybibliography/"> <input type="hidden" name="csrfmiddlewaretoken" value="Hr3Bicli5ECCEJn2kRckXawX6RP7QNpb1AV0DWVcwN3h8T7TLCI5zoyikWOKxspU"> <div class="action-panel-control-wrap bibliographies-controls"> <div class="choice-group"> <ul class="bibliographies-action-add radio-group-items"> <li> <input name="bibliography" id="my-bibliography" class="my-bibliography" type="radio" checked/> <label for="my-bibliography">My Bibliography</label> </li> </ul> </div> </div> <div class="bibliographies-retry-load-on-error usa-input-error-message selection-validation-message"> Unable to load your delegates due to an error<br> <a href="#">Please try again</a> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Adding..." data-pinger-ignore> Add </button> <button class="action-panel-cancel" aria-label="Close 'Add to bibliography' panel" ref="linksrc=close_bibliography_panel" aria-controls="bibliography-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="mybib" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="saved-search-action-panel" class="saved-search-action-panel action-panel " aria-hidden="true" data-saved-search-open-panel-enabled="false" data-saved-search-open-panel-url-hash="#open-saved-search-panel"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Your saved search </h2> <form id="saved-search-action-panel-form" class="saved-search-action-panel-form action-panel-content action-form" data-create-saved-search-url="/create-saved-search/" data-try-search-terms-url="/try-search-term/" data-saved-search-root-url="https://www.ncbi.nlm.nih.gov/myncbi/searches/"> <input type="hidden" name="csrfmiddlewaretoken" value="Hr3Bicli5ECCEJn2kRckXawX6RP7QNpb1AV0DWVcwN3h8T7TLCI5zoyikWOKxspU"> <div class="action-panel-control-wrap"> <label for="saved-search-name" class="action-panel-label saved-search-name-label required-field-asterisk"> Name of saved search: </label> <input maxlength="200" type="text" name="saved-search-name" id="saved-search-name" class="saved-search-name" value="" required pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/"> </div> <div class="action-panel-control-wrap"> <label for="saved-search-term" class="action-panel-label required-field-asterisk"> Search terms: </label> <textarea name="saved-search-term" id="saved-search-term" class="saved-search-term" required></textarea> </div> <div class="test-search-term-wrap"> <a href="#" class="try-search-term">Test search terms</a> </div> <div class="choice-group action-panel-extra-margin-top"> <span class="action-panel-label" id="fieldset-label"> Would you like email updates of new search results? </span> <fieldset id="saved-search-alert" aria-describedby="fieldset-label"> <legend class="usa-sr-only">Saved Search Alert Radio Buttons</legend> <ul class="radio-group-items"> <li> <input type="radio" id="saved-search-alert-yes" class="saved-search-alert-yes" name="saved-search-alert" value="yes" checked> <label for="saved-search-alert-yes" class="action-panel-label">Yes</label> </li> <li> <input aria-label="No radio input" type="radio" id="saved-search-alert-no" class="saved-search-alert-no" name="saved-search-alert" value="no"> <label for="saved-search-alert-no" class="action-panel-label">No</label> </li> </ul> </fieldset> </div> <div class="alert-schedule-wrap"> <div class="action-panel-control-wrap"> <label class="action-panel-label"> Email: </label> <span aria-label="Email address" id="saved-search-email" class="action-panel-label"><span class="action-panel-label-bold"></span> (<a class="myncbi-account-settings" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/account/settings/">change</a>)</span> </div> <div class="action-panel-control-wrap action-panel-extra-margin-top"> <label for="saved-search-frequency" class="action-panel-label"> Frequency: </label> <select id="saved-search-frequency" class="no-border-panel-selector saved-search-frequency"> <option value="monthly">Monthly</option> <option value="weekly">Weekly</option> <option value="daily">Daily</option> </select> </div> <div class="action-panel-control-wrap saved-search-monthly-additional"> <label for="saved-search-monthly-on-day" class="action-panel-label"> Which day? </label> <select id="saved-search-monthly-on-day" class="no-border-panel-selector"> <option value="Sunday">The first Sunday</option> <option value="Monday">The first Monday</option> <option value="Tuesday">The first Tuesday</option> <option value="Wednesday">The first Wednesday</option> <option value="Thursday">The first Thursday</option> <option value="Friday">The first Friday</option> <option value="Saturday">The first Saturday</option> <option value="day">The first day</option> <option value="weekday">The first weekday</option> </select> </div> <div class="action-panel-control-wrap saved-search-weekly-additional"> <label for="saved-search-weekly-on-day" class="action-panel-label"> Which day? </label> <select id="saved-search-weekly-on-day" class="no-border-panel-selector saved-search-weekly-on-day"> <option value="Sunday">Sunday</option> <option value="Monday">Monday</option> <option value="Tuesday">Tuesday</option> <option value="Wednesday">Wednesday</option> <option value="Thursday">Thursday</option> <option value="Friday">Friday</option> <option value="Saturday">Saturday</option> </select> </div> <div class="action-panel-control-wrap"> <label for="saved-search-report" class="action-panel-label"> Report format: </label> <select id="saved-search-report" class="no-border-panel-selector saved-search-report"> <option value="DocSum">Summary</option> <option value="DocSumText">Summary (text)</option> <option value="Abstract">Abstract</option> <option value="AbstractText">Abstract (text)</option> <option value="MEDLINE">PubMed</option> </select> </div> <div class="action-panel-control-wrap"> <label for="saved-search-amount" class="action-panel-label"> Send at most: </label> <select id="saved-search-amount" class="no-border-panel-selector saved-search-amount"> <option value="1">1 item</option> <option value="5" selected>5 items</option> <option value="10">10 items</option> <option value="20">20 items</option> <option value="50">50 items</option> <option value="100">100 items</option> <option value="200">200 items</option> </select> </div> <div> <input type="checkbox" id="saved-search-send-if-no-result" class="saved-search-send-if-no-result" name="saved-search-send-if-no-result"> <label for="saved-search-send-if-no-result" class="action-panel-label smaller-checkbox"> Send even when there aren't any new results </label> </div> <div class="action-panel-control-wrap option-text-in-email-wrap"> <label for="saved-search-email-text" class="action-panel-label"> Optional text in email: </label> <textarea name="saved-search-email-text" id="saved-search-email-text" class="saved-search-email-text"></textarea> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Saving..." data-ga-category="save_share" data-ga-action="alert" data-ga-label="save"> Save </button> <button class="action-panel-cancel" aria-label="Close 'Your saved search' panel" ref="linksrc=close_saved_search_panel" aria-controls="saved-search-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="alert" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="citation-manager-action-panel" class="citation-manager-action-panel action-panel" aria-hidden="true"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Create a file for external citation management software </h2> <form id="citation-manager-action-panel-form" class="action-panel-content action-form" action="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/results-export-ids/" data-by-search-action="/results-export-search-data/" data-by-ids-action="/results-export-ids/" method="post" data-by-search-method="post" data-by-ids-method="post"> <input type="hidden" name="csrfmiddlewaretoken" value="Hr3Bicli5ECCEJn2kRckXawX6RP7QNpb1AV0DWVcwN3h8T7TLCI5zoyikWOKxspU"> <input name="results-format" type="hidden" value="pubmed"/> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Sending..." data-ga-category="save_share" data-ga-action="citation_manager" data-ga-label="save"> Create file </button> <button class="action-panel-cancel" aria-label="Close 'Send citations to citation manager' panel" ref="linksrc=close_citation_manager_panel" aria-controls="citation-manager-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="citation_manager" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="rss-action-panel" class="rss-action-panel action-panel " aria-hidden="true"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Your RSS Feed </h2> <form id="rss-action-panel-form" class="rss-action-panel-form action-panel-content action-form" data-create-rss-feed-url="/create-rss-feed-url/" data-search-form-term-value=""> <input type="hidden" name="csrfmiddlewaretoken" value="Hr3Bicli5ECCEJn2kRckXawX6RP7QNpb1AV0DWVcwN3h8T7TLCI5zoyikWOKxspU"> <div class="action-panel-control-wrap"> <label for="rss-name" class="action-panel-label required-field-asterisk"> Name of RSS Feed: </label> <input maxlength="200" placeholder="Name" type="text" name="rss-name" id="rss-name" class="rss-name" value="" required pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/"> </div> <div class="rss-limit-wrap"> <div class="action-panel-control-wrap action-panel-extra-margin-top"> <label for="rss-limit" class="action-panel-label"> Number of items displayed: </label> <select id="rss-limit" class="no-border-panel-selector rss-limit"> <option value="5">5</option> <option value="10">10</option> <option value="15" selected="selected">15</option> <option value="20">20</option> <option value="50">50</option> <option value="100">100</option> </select> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Creating..." data-ga-category="save_share" data-ga-action="alert" data-ga-label="save"> Create RSS </button> <button class="action-panel-cancel" aria-label="Close 'Your RSS' panel" ref="linksrc=close_rss_panel" aria-controls="rss-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="alert" data-ga-label="cancel"> Cancel </button> </div> <div class="action-panel-control-wrap rss-link-copy-wrap"> <label for="rss-link" class="usa-sr-only">RSS Link</label> <input placeholder="Your RSS Feed Link" type="text" name="rss-link" id="rss-link" class="rss-link" title="RSS Link"> <button type="button" disabled class="rss-link-copy-button disabled" data-ga-category="save_share" data-ga-action="rss" data-ga-label="copy"> Copy </button> </div> </form> </div> </div> </div> </div> <div class="article-page" id="article-page" data-article-pmid="21745365"> <aside class="page-sidebar"> <div class="inner-wrap"> <div class="full-text-links"> <div class="full-view"> <h3 class="title"> Full text links </h3> <div class="full-text-links-list"> <a class="link-item dialog-focus" href="https://web.archive.org/web/20240427130614/https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-9-108" target="_blank" rel="noopener" ref="linksrc=fulltextorjournal_fulltext&is_pmc=False&PrId=3196&itool=Abstract-def&log$=linkouticon&uid=21745365&db=pubmed&nlmid=101190741" title="See full text options at BioMed Central" data-ga-category="full_text" data-ga-action="BioMed Central" data-ga-label="21745365"><img src="https://web.archive.org/web/20240427130614im_/https://cdn.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--s3-service-broker-live-ddda94b7-dbb0-4917-917d-776dae91ebba.s3.amazonaws.com-bmc-linkout-fulltext.png" alt="full text provider logo"><span class="text"> BioMed Central </span></a><a class="link-item pmc " href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21745365/" target="_blank" rel="noopener" ref="linksrc=fulltextorjournal_fulltext&is_pmc=True&PrId=3494&itool=Abstract-def&log$=linkouticon&uid=21745365&db=pubmed&nlmid=101190741" title="Free full text at PubMed Central" data-ga-category="full_text" data-ga-action="PMC" data-ga-label="21745365"><span class="text"> Free PMC article </span></a> </div> </div> <div class="short-view"> <a href="#" class="full-text-links-button full-text-links-dialog-trigger"> Full text links </a> </div> </div> <div class="actions-buttons sidebar"><h3 class="title">Actions</h3><div class="inner-wrap"><button class="citation-button citation-dialog-trigger" aria-label="Open dialog with citation text in different styles" data-ga-category="save_share" data-ga-action="cite" data-ga-label="open" data-all-citations-url="/21745365/citations/" data-citation-style="nlm" data-pubmed-format-link="/21745365/export/"><span class="button-label">Cite</span></button><link type="text/css" href="ncbi-overlay-block/src/overlay-block.css"><div class="collections-button-container" data-article-id="21745365" data-article-db="pubmed"><button class="collections-button collections-dialog-trigger" aria-label="Save article in MyNCBI collections." data-ga-category="collections_button" data-ga-action="click" data-ga-label="collections_button" data-collections-open-dialog-enabled="false" data-collections-open-dialog-url="https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F21745365%2F%23open-collections-dialog" data-in-collections="false"><span class="button-label">Collections</span></button><div class="overlay" role="dialog"><div id="collections-action-dialog" class="dialog collections-dialog" aria-hidden="true"><div class="title">Add to Collections</div><div class="collections-action-panel action-panel"><form id="collections-action-dialog-form" class="collections-action-panel-form action-panel-content action-form action-panel-smaller-selectors" data-existing-collections-url="/list-existing-collections/" data-add-to-existing-collection-url="/add-to-existing-collection/" data-create-and-add-to-new-collection-url="/create-and-add-to-new-collection/" data-myncbi-max-collection-name-length="100" data-collections-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/"><input type="hidden" name="csrfmiddlewaretoken" value="Hr3Bicli5ECCEJn2kRckXawX6RP7QNpb1AV0DWVcwN3h8T7TLCI5zoyikWOKxspU"><div class="choice-group" role="radiogroup"><ul class="radio-group-items"><li><input type="radio" id="collections-action-dialog-new" class="collections-new" name="collections" value="new" data-ga-category="collections_button" data-ga-action="click" data-ga-label="collections_radio_new"><label for="collections-action-dialog-new">Create a new collection</label></li><li><input type="radio" id="collections-action-dialog-existing" class="collections-existing" name="collections" value="existing" checked="true" data-ga-category="collections_button" 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Search </a></li></ul></div></div></div><span class="period">. </span><span class="cit">2011 Jul 11:9:108.</span> </div> <span class="citation-doi"> doi: 10.1186/1479-5876-9-108. </span> </div> <h1 class="heading-title"> A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols </h1> <div class="inline-authors"> <div class="authors"> <div class="authors-list"> <span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Attig+S&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Sebastian Attig">Sebastian Attig</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Division of Translational and Experimental Oncology, Department of Internal Medicine III, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany." href="#full-view-affiliation-1" ref="linksrc=author_aff"> 1 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Price+L&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Leah Price">Leah Price</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Janetzki+S&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Sylvia Janetzki">Sylvia Janetzki</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Kalos+M&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Michael Kalos">Michael Kalos</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Pride+M&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Michael Pride">Michael Pride</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=McNeil+L&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Lisa McNeil">Lisa McNeil</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Clay+T&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Tim Clay">Tim Clay</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Yuan+J&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Jianda Yuan">Jianda Yuan</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Odunsi+K&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Kunle Odunsi">Kunle Odunsi</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Hoos+A&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Axel Hoos">Axel Hoos</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Romero+P&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Pedro Romero">Pedro Romero</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Britten+CM&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Cedrik M Britten">Cedrik M Britten</a><span class="semicolon">; </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=CRI-CIC+Assay+Working+Group%5BCorporate+Author%5D" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="CRI-CIC Assay Working Group">CRI-CIC Assay Working Group</a></span> </div> </div> </div> <div class="short-article-details"> Affiliations 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HLA-peptide multimer staining protocols </h1> <div class="short-citation"> <span class="authors-list"> <span class="authors-list-item "><span class="full-name">Sebastian Attig</span><span class="citation-separator"> et al.</span></span> </span> <span class="citation-journal"> J Transl Med<span class="citation-separator">.</span> </span> <span class="date"> <time class="citation-year" datetime="2011">2011</time><span class="citation-separator">.</span> </span> </div> <div class="more-article-details-section with-display-settings"> <button class="more-article-details" id="toggle-article-details" data-alt-text="Hide details" ref="linksrc=toggle_article_details" aria-controls="full-article-details" aria-expanded="false" data-fetch-url="/21745365/long-authors/" data-id-prefix=""> <span class="title">Show details</span> </button> <div class="display-options" data-format-key="format" data-default-format-value="abstract" data-current-format-value="abstract" role="region"> <button 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ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Sebastian Attig">Sebastian Attig</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Division of Translational and Experimental Oncology, Department of Internal Medicine III, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany." href="#short-view-affiliation-1" ref="linksrc=author_aff"> 1 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Price+L&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Leah Price">Leah Price</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Janetzki+S&cauthor_id=21745365" 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data-ga-category="search" data-ga-action="author_link" data-ga-label="Lisa McNeil">Lisa McNeil</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Clay+T&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Tim Clay">Tim Clay</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Yuan+J&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Jianda Yuan">Jianda Yuan</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Odunsi+K&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Kunle Odunsi">Kunle Odunsi</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Hoos+A&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Axel Hoos">Axel Hoos</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Romero+P&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Pedro Romero">Pedro Romero</a><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=Britten+CM&cauthor_id=21745365" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Cedrik M Britten">Cedrik M Britten</a><span class="semicolon">; </span></span><span class="authors-list-item "><a class="full-name" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=CRI-CIC+Assay+Working+Group%5BCorporate+Author%5D" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="CRI-CIC Assay Working Group">CRI-CIC Assay Working Group</a></span> </div> </div> <div class="affiliations"> <h3 class="title"> Affiliation </h3> <ul class="item-list"> <li data-affiliation-id="short-view-affiliation-1" id="short-view-affiliation-1"><sup class="key">1</sup> Division of Translational and Experimental Oncology, Department of Internal Medicine III, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany.</li> </ul> </div> </div> <ul class="identifiers" id="short-view-identifiers"> <li> <span class="identifier pubmed"> <span class="id-label"> PMID: </span> <strong class="current-id" title="PubMed ID">21745365</strong> </span> </li> <li> <span class="identifier pmc"> <span class="id-label"> PMCID: </span> <a class="id-link" target="_blank" rel="noopener" ref="linksrc=article_id_link&article_id=PMC3148571&id_type=PMC" href="https://web.archive.org/web/20240427130614/http://www.ncbi.nlm.nih.gov/pmc/articles/pmc3148571/" data-ga-category="full_text" data-ga-action="PMCID"> PMC3148571 </a> </span> </li> <li> <span class="identifier doi"> <span class="id-label"> DOI: </span> <a class="id-link" target="_blank" rel="noopener" ref="linksrc=article_id_link&article_id=10.1186/1479-5876-9-108&id_type=DOI" href="https://web.archive.org/web/20240427130614/https://doi.org/10.1186/1479-5876-9-108" data-ga-category="full_text" data-ga-action="DOI"> 10.1186/1479-5876-9-108 </a> </span> </li> </ul> <div class="in-clipboard-label " hidden> Item in Clipboard </div> </div> </div> </header> <div class="actions-buttons inline"><div class="inner-wrap"><button class="full-text-links-button full-text-links-dialog-trigger" title="Open dialog with full text links" ref="linksrc=full_text_links"> Full text links </button><button class="citation-button citation-dialog-trigger" aria-label="Open dialog with citation text in different styles" data-ga-category="save_share" data-ga-action="cite" data-ga-label="open" data-all-citations-url="/21745365/citations/" data-citation-style="nlm" data-pubmed-format-link="/21745365/export/"><span class="button-label">Cite</span></button><button class="more-actions-button more-actions-dialog-trigger" title="Open dialog with more actions to take" ref="linksrc=more_actions_btn"></button><div class="display-options" data-format-key="format" data-default-format-value="abstract" data-current-format-value="abstract" role="region"><button class="trigger usa-button" title="Change format" data-ga-category="display_options" data-ga-action="display_options_dialog"><span class="button-label">Display options</span></button><div class="dropdown" hidden><div class="title"> Display options </div><div class="content"><div class="format-container"><span class="option-label"> Format </span><select name="format" data-initial-value="abstract" aria-label="Display format" id="inline-actions-display-format"><option value="abstract">Abstract</option><option value="pubmed">PubMed</option><option value="pmid">PMID</option></select></div></div></div></div></div></div> <div class="abstract" id="abstract"> <h2 class="title"> Abstract </h2> <div class="abstract-content selected" id="eng-abstract"> <p> <strong class="sub-title"> Background: </strong> The introduction of antibody markers to identify undesired cell populations in flow-cytometry based assays, so called DUMP channel markers, has become a practice in an increasing number of labs performing HLA-peptide multimer assays. However, the impact of the introduction of a DUMP channel in multimer assays has so far not been systematically investigated across a broad variety of protocols. </p> <p> <strong class="sub-title"> Methods: </strong> The Cancer Research Institute's Cancer Immunotherapy Consortium (CRI-CIC) conducted a multimer proficiency panel with a specific focus on the impact of DUMP channel use. The panel design allowed individual laboratories to use their own protocol for thawing, staining, gating, and data analysis. Each experiment was performed twice and in parallel, with and without the application of a dump channel strategy. </p> <p> <strong class="sub-title"> Results: </strong> The introduction of a DUMP channel is an effective measure to reduce the amount of non-specific MULTIMER binding to T cells. Beneficial effects for the use of a DUMP channel were observed across a wide range of individual laboratories and for all tested donor-antigen combinations. In 48% of experiments we observed a reduction of the background MULTIMER-binding. In this subgroup of experiments the median background reduction observed after introduction of a DUMP channel was 0.053%. </p> <p> <strong class="sub-title"> Conclusions: </strong> We conclude that appropriate use of a DUMP channel can significantly reduce background staining across a large fraction of protocols and improve the ability to accurately detect and quantify the frequency of antigen-specific T cells by multimer reagents. Thus, use of a DUMP channel may become crucial for detecting low frequency antigen-specific immune responses. Further recommendations on assay performance and data presentation guidelines for publication of MULTIMER experimental data are provided. </p> </div> </div> <p class="disclaimer" id="disclaimer"> <a href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/disclaimer/" target="_blank" ga_category="literature_resources" ga_action="disclaimer_link" ga_label="under_abstract">PubMed Disclaimer</a> </p> <div class="figures" id="figures"> <h2 class="title"> Figures </h2> <div class="figures-list" id="slides-container" itemscope itemtype="http://schema.org/ImageGallery"> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="0" data-label-slug="figure-1"> <a class="figure-link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3148571/bin/1479-5876-9-108-1.jpg" itemprop="contentUrl" aria-describedby="figure-caption-0" role="button" data-image-width="600" data-image-height="732" data-image-alt="Figure 1" data-pmc-id="PMC3148571" data-figure-id="F1"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-0" src="https://web.archive.org/web/20240427130614im_/https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3148571/bin/1479-5876-9-108-1.gif" alt="Figure 1"/> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="600"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="732"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 1 </p> </strong> <div class="figure-caption-contents"><b> MULTIMER binding in the condition… </b></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 1 </p> </strong> <div class="figure-caption-contents"><b> MULTIMER binding in the condition WITHOUT versus WITH use of a DUMP channel… </b></div> </div> <figcaption id="figure-caption-0" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 1 </strong> <div class="figure-caption-contents"><b>MULTIMER binding in the condition WITHOUT versus WITH use of a DUMP channel</b>. The figure shows results for the percentage of MULTIMER-positive CD8-positive events in the condition WITHOUT DUMP (x-axis) and WITH DUMP (y-axis) for (a) the seven positive donor-antigen combinations after staining with the CMV- or Melan-A MULTIMER and (b) the negative donor antigen combination (CMV in D2) as well as the results generated when using the irrelevant MULTIMERS (D1 to D4). Experiments with an increase (> 0.01%) of non-specific MUTIMER binding in the condition with DUMP are shown in red. Experiments with a decrease (> 0.01%) of non-specific MULTIMER binding in the condition with DUMP are shown in green.</div> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="1" data-label-slug="figure-2"> <a class="figure-link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3148571/bin/1479-5876-9-108-2.jpg" itemprop="contentUrl" aria-describedby="figure-caption-1" role="button" data-image-width="600" data-image-height="436" data-image-alt="Figure 2" data-pmc-id="PMC3148571" data-figure-id="F2"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-1" src="https://web.archive.org/web/20240427130614im_/https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3148571/bin/1479-5876-9-108-2.gif" alt="Figure 2"/> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="600"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="436"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 2 </p> </strong> <div class="figure-caption-contents"><b> <i> In silico </i> study </b> : The…</div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 2 </p> </strong> <div class="figure-caption-contents"><b> <i> In silico </i> study </b> : The figure shows the frequency of events detected in…</div> </div> <figcaption id="figure-caption-1" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 2 </strong> <div class="figure-caption-contents"><b><i>In silico</i> study</b>: The figure shows the frequency of events detected in the MULTIMER-positive CD8-positive fraction when neither DUMP channel markers nor dead cell dyes were included in the gating strategy (x-axis) and the four corresponding event counts on the y-axis in the gating strategy NO DEAD and NO DUMP (blue), WITH DEAD and NO DUMP (black), NO DEAD and WITH DUMP (red), WITH DEAD and WITH DUMP (green). The figure also shows the resulting linear regression curves for each of the four data sets.</div> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="2" data-label-slug="figure-3"> <a class="figure-link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3148571/bin/1479-5876-9-108-3.jpg" itemprop="contentUrl" aria-describedby="figure-caption-2" role="button" data-image-width="600" data-image-height="995" data-image-alt="Figure 3" data-pmc-id="PMC3148571" data-figure-id="F3"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-2" src="https://web.archive.org/web/20240427130614im_/https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3148571/bin/1479-5876-9-108-3.gif" alt="Figure 3"/> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="600"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="995"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 3 </p> </strong> <div class="figure-caption-contents"><b> MULTIMER binding to CD8-positive cells… </b></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 3 </p> </strong> <div class="figure-caption-contents"><b> MULTIMER binding to CD8-positive cells versus MULTIMER binding to CD8-negative cells </b> . (a)…</div> </div> <figcaption id="figure-caption-2" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 3 </strong> <div class="figure-caption-contents"><b>MULTIMER binding to CD8-positive cells versus MULTIMER binding to CD8-negative cells</b>. (a) The Figure displays the percentage of MULTIMER binding to CD8-negative cells (y-axis) versus the percentage of MULTIMER binding to CD8-positive cells (x-axis) for each staining from a positive donor-antigen combination (DUMP and NO DUMP). (b) The four dot plots illustrate representative experiment results with a high background (left column) and a low background (right column) for the CMV-pp65 MULTIMER (upper row) and the Melan-A MULTIMER (lower row).</div> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="3" data-label-slug="figure-4"> <a class="figure-link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3148571/bin/1479-5876-9-108-4.jpg" itemprop="contentUrl" aria-describedby="figure-caption-3" role="button" data-image-width="600" data-image-height="442" data-image-alt="Figure 4" data-pmc-id="PMC3148571" data-figure-id="F4"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-3" src="https://web.archive.org/web/20240427130614im_/https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3148571/bin/1479-5876-9-108-4.gif" alt="Figure 4"/> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="600"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="442"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 4 </p> </strong> <div class="figure-caption-contents"><b> Expanded CIC HLA-Peptide Multimer Harmonization… </b></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 4 </p> </strong> <div class="figure-caption-contents"><b> Expanded CIC HLA-Peptide Multimer Harmonization Guidelines </b> .</div> </div> <figcaption id="figure-caption-3" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 4 </strong> <div class="figure-caption-contents"><b>Expanded CIC HLA-Peptide Multimer Harmonization Guidelines</b>.</div> </figcaption> </figure> </div>  <div class="pswp figures-dialog" id="figures-dialog" tabindex="-1" role="dialog" aria-hidden="true" data-image-gallery-uid="article-figures" aria-label="Figures Dialog">  <div class="pswp__bg"></div>  <div class="pswp__scroll-wrap">  <div class="pswp__container"> <div class="pswp__item"></div> <div class="pswp__item"></div> <div class="pswp__item"></div> </div>  <div class="pswp__ui pswp__ui--hidden"> <div class="pswp__top-bar">  <div class="pswp__counter"></div> <button class="pswp__button pswp__button--close" title="Close (Esc)"></button> <button class="pswp__button pswp__button--share" title="Share"></button> <button class="pswp__button pswp__button--fs" title="Toggle fullscreen"></button> <button class="pswp__button pswp__button--zoom" title="Zoom in/out"></button>   <div class="pswp__preloader"> <div class="pswp__preloader__icn"> <div class="pswp__preloader__cut"> <div class="pswp__preloader__donut"></div> </div> </div> </div> </div> <div class="pswp__share-modal pswp__share-modal--hidden pswp__single-tap"> <div class="pswp__share-tooltip"></div> </div> <button class="pswp__button pswp__button--arrow--left" title="Previous (arrow left)"> </button> <button class="pswp__button pswp__button--arrow--right" title="Next (arrow right)"> </button> <div class="pswp__caption"> <div class="pswp__caption__center"></div> </div> </div> </div> </div> <div class="figure-dialog-caption"> <div class="figure-caption-link-container"> <a class="figure-caption-link" href="#">See this image and copyright information in PMC</a> </div> <div class="figure-caption-center" data-expand-title="click to show full caption" data-collapse-title="click to hide full caption"> </div> <button class="figure-caption-toggle-btn" aria-label="show full caption" data-expand-text="show full caption" data-collapse-text="hide full caption">  </button> </div> </div> <div class="similar-articles" id="similar"> <h2 class="title"> Similar articles </h2> <ul class="articles-list" id="similar-articles-list"> <li class="full-docsum"> <div class="docsum-content" style="display:inline;"> <a class="docsum-title" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/27363684/" ref="article_id=27363684&linksrc=similar_articles_link&ordinalpos=1" data-ga-category="similar_article" data-ga-action="27363684" data-ga-label=""> Validation of Immunomonitoring Methods for Application in Clinical Studies: The HLA-Peptide Multimer Staining Assay. </a> <div class="docsum-citation full-citation"> <span class="docsum-authors full-authors">Chandran PA, Laske K, Cazaly A, Rusch E, Schmid-Horch B, Rammensee HG, Ottensmeier CH, Gouttefangeas C.</span> <span class="docsum-authors short-authors">Chandran PA, et al.</span> <span class="docsum-journal-citation full-journal-citation">Cytometry B Clin Cytom. 2018 Mar;94(2):342-353. doi: 10.1002/cyto.b.21397. 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Report of the third immunology of diabetes society T-cell workshop. J Autoimmun. 2003;21:365–376. doi: 10.1016/S0896-8411(03)00111-2. - <a class="reference-link" href="https://web.archive.org/web/20240427130614/https://doi.org/10.1016/s0896-8411(03)00111-2" ref="linksrc=references_link&ordinalpos=1" data-ga-category="reference" data-ga-action="10.1016/S0896-8411(03)00111-2"> DOI </a> - <a class="reference-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/14624759/" ref="linksrc=references_link&ordinalpos=2" data-ga-category="reference" data-ga-action="14624759"> PubMed </a> </li> </ol> </li> <li> <ol class="references-and-notes-list"> <li class="skip-numbering" value="1"> Cox JH, Ferrari G, Kalams SA, Lopaczynski W, Oden N, D'souza MP. Results of an ELISPOT proficiency panel conducted in 11 laboratories participating in international human immunodeficiency virus type 1 vaccine trials. AIDS Res Hum Retroviruses. 2005;21:68–81. doi: 10.1089/aid.2005.21.68. - <a class="reference-link" href="https://web.archive.org/web/20240427130614/https://doi.org/10.1089/aid.2005.21.68" ref="linksrc=references_link&ordinalpos=1" data-ga-category="reference" data-ga-action="10.1089/aid.2005.21.68"> DOI </a> - <a class="reference-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/15665646/" ref="linksrc=references_link&ordinalpos=2" data-ga-category="reference" data-ga-action="15665646"> PubMed </a> </li> </ol> </li> <li> <ol class="references-and-notes-list"> <li class="skip-numbering" value="1"> Janetzki S, Cox JH, Oden N, Ferrari G. Standardization and validation issues of the ELISPOT assay. Methods Mol Biol. 2005;302:51–86. - <a class="reference-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/15937345/" ref="linksrc=references_link&ordinalpos=1" data-ga-category="reference" data-ga-action="15937345"> PubMed </a> </li> </ol> </li> <li> <ol class="references-and-notes-list"> <li class="skip-numbering" value="1"> Maecker HT, Rinfret A, D'Souza P, Darden J, Roig E, Landry C. et al.Standardization of cytokine flow cytometry assays. BMC Immunol. 2005;6:13. - <a class="reference-link" href="https://web.archive.org/web/20240427130614/http://www.ncbi.nlm.nih.gov/pmc/articles/pmc1184077/" ref="linksrc=references_link&ordinalpos=1" data-ga-category="reference" data-ga-action="PMC1184077"> PMC </a> - <a class="reference-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/15978127/" ref="linksrc=references_link&ordinalpos=2" data-ga-category="reference" data-ga-action="15978127"> PubMed </a> </li> </ol> </li> <li> <ol class="references-and-notes-list"> <li class="skip-numbering" value="1"> Britten CM, Janetzki S, van der Burg SH, Gouttefangeas C, Hoos A. Toward the harmonization of immune monitoring in clinical trials: Quo vadis? Cancer Immunol Immunother. 2007;57:285–288. doi: 10.1007/s00262-007-0379-z. - <a class="reference-link" href="https://web.archive.org/web/20240427130614/https://doi.org/10.1007/s00262-007-0379-z" ref="linksrc=references_link&ordinalpos=1" data-ga-category="reference" data-ga-action="10.1007/s00262-007-0379-z"> DOI </a> - <a class="reference-link" href="https://web.archive.org/web/20240427130614/http://www.ncbi.nlm.nih.gov/pmc/articles/pmc2150641/" ref="linksrc=references_link&ordinalpos=2" data-ga-category="reference" data-ga-action="PMC2150641"> PMC </a> - <a class="reference-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/17721782/" ref="linksrc=references_link&ordinalpos=3" data-ga-category="reference" data-ga-action="17721782"> PubMed </a> </li> </ol> </li> </ol> <div class="actions-bar"> <button class="show-all" ref="linksrc=show_all_references_link" aria-controls="top-references-list-1" data-next-page-url="/21745365/references/" data-ga-category="reference" 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aria-controls="keyword-actions-mesh-terms-major-6" aria-expanded="false" data-pinger-ignore> HLA Antigens / metabolism* </button><div id="keyword-actions-mesh-terms-major-6" class="keyword-actions-dropdown dropdown dropdown-container" hidden aria-label="Dropdown menu for keyword HLA Antigens / metabolism*"><div class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=%22HLA+Antigens%2Fmetabolism%22%5BMAJR%5D&sort=date&sort_order=desc" data-href="/?term=%22HLA+Antigens%2Fmetabolism%22%5BMAJR%5D&sort=date&sort_order=desc" ref="linksrc=search_in_pubmed_keyword_link&mesh="HLA Antigens/metabolism"[MAJR]" data-ga-category="search" data-ga-action="mesh_term" data-ga-label="HLA Antigens / metabolism*"> Search in PubMed </a></li><li><a class="search-in-mesh-link dropdown-block-link" ref="linksrc=search_in_mesh_link" 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class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=%22Humans%22%5BMeSH%5D&sort=date&sort_order=desc" data-href="/?term=%22Humans%22%5BMeSH%5D&sort=date&sort_order=desc" ref="linksrc=search_in_pubmed_keyword_link&mesh="Humans"[MeSH]" data-ga-category="search" data-ga-action="mesh_term" data-ga-label="Humans"> Search in PubMed </a></li><li><a class="search-in-mesh-link dropdown-block-link" ref="linksrc=search_in_mesh_link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/mesh?term=Humans" data-ga-category="search_mesh" data-ga-action="mesh_term_link" data-ga-label="Humans"> Search in MeSH </a></li><li><a class="add-to-search-link dropdown-block-link" ref="linksrc=add_to_search_link" role="button" data-search-term=""Humans"[MeSH]" data-ga-category="search" data-ga-action="add_to_search" 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href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/mesh?term=Reproducibility+of+Results" data-ga-category="search_mesh" data-ga-action="mesh_term_link" data-ga-label="Reproducibility of Results"> Search in MeSH </a></li><li><a class="add-to-search-link dropdown-block-link" ref="linksrc=add_to_search_link" role="button" data-search-term=""Reproducibility of Results"[MeSH]" data-ga-category="search" data-ga-action="add_to_search" data-ga-label=""Reproducibility of Results"[MeSH]" href="#"> Add to Search </a></li></ul></div></div></div></li><li><div class="keyword-actions dropdown-block"><button class="keyword-actions-trigger trigger keyword-link major" aria-label="Toggle dropdown menu for keyword Staining and Labeling / methods*" tabindex="0" aria-controls="keyword-actions-mesh-terms-major-13" aria-expanded="false" data-pinger-ignore> Staining and Labeling / methods* </button><div id="keyword-actions-mesh-terms-major-13" class="keyword-actions-dropdown dropdown dropdown-container" hidden aria-label="Dropdown menu for keyword Staining and Labeling / methods*"><div class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=%22Staining+and+Labeling%2Fmethods%22%5BMAJR%5D&sort=date&sort_order=desc" data-href="/?term=%22Staining+and+Labeling%2Fmethods%22%5BMAJR%5D&sort=date&sort_order=desc" ref="linksrc=search_in_pubmed_keyword_link&mesh="Staining and Labeling/methods"[MAJR]" data-ga-category="search" data-ga-action="mesh_term" data-ga-label="Staining and Labeling / methods*"> Search in PubMed </a></li><li><a class="search-in-mesh-link dropdown-block-link" ref="linksrc=search_in_mesh_link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/mesh?term=Staining+and+Labeling" data-ga-category="search_mesh" data-ga-action="mesh_term_link" data-ga-label="Staining and Labeling / methods*"> Search in MeSH </a></li><li><a class="add-to-search-link dropdown-block-link" ref="linksrc=add_to_search_link" role="button" data-search-term=""Staining and Labeling/methods"[MAJR]" data-ga-category="search" data-ga-action="add_to_search" data-ga-label=""Staining and Labeling/methods"[MAJR]" href="#"> Add to Search </a></li></ul></div></div></div></li><li><div class="keyword-actions dropdown-block"><button class="keyword-actions-trigger trigger keyword-link" aria-label="Toggle dropdown menu for keyword Subcellular Fractions / metabolism" tabindex="0" aria-controls="keyword-actions-mesh-terms-14" aria-expanded="false" data-pinger-ignore> Subcellular Fractions / metabolism </button><div id="keyword-actions-mesh-terms-14" class="keyword-actions-dropdown dropdown dropdown-container" hidden aria-label="Dropdown menu for keyword Subcellular Fractions / metabolism"><div class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=%22Subcellular+Fractions%2Fmetabolism%22%5BMeSH%5D&sort=date&sort_order=desc" data-href="/?term=%22Subcellular+Fractions%2Fmetabolism%22%5BMeSH%5D&sort=date&sort_order=desc" ref="linksrc=search_in_pubmed_keyword_link&mesh="Subcellular Fractions/metabolism"[MeSH]" data-ga-category="search" data-ga-action="mesh_term" data-ga-label="Subcellular Fractions / metabolism"> Search in PubMed </a></li><li><a class="search-in-mesh-link dropdown-block-link" ref="linksrc=search_in_mesh_link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/mesh?term=Subcellular+Fractions" data-ga-category="search_mesh" data-ga-action="mesh_term_link" data-ga-label="Subcellular Fractions / metabolism"> Search in MeSH </a></li><li><a class="add-to-search-link dropdown-block-link" ref="linksrc=add_to_search_link" role="button" data-search-term=""Subcellular Fractions/metabolism"[MeSH]" data-ga-category="search" data-ga-action="add_to_search" data-ga-label=""Subcellular Fractions/metabolism"[MeSH]" href="#"> Add to Search </a></li></ul></div></div></div></li></ul> </div> <div id="substances" class="substances keywords-section"> <h2 class="title"> Substances </h2> <ul class="keywords-list"><li><div class="keyword-actions dropdown-block"><button class="keyword-actions-trigger trigger keyword-link" aria-label="Toggle dropdown menu for keyword Biomarkers" tabindex="0" aria-controls="keyword-actions-substances-0" aria-expanded="false" data-pinger-ignore> Biomarkers </button><div id="keyword-actions-substances-0" class="keyword-actions-dropdown dropdown dropdown-container" hidden aria-label="Dropdown menu for keyword Biomarkers"><div class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=%22Biomarkers%22%5Bnm%5D&sort=date&sort_order=desc" data-href="/?term=%22Biomarkers%22%5Bnm%5D&sort=date&sort_order=desc" ref="linksrc=search_in_pubmed_keyword_link&keyword="Biomarkers"[nm]" data-ga-category="search" data-ga-action="substance" data-ga-label="Biomarkers"> Search in PubMed </a></li><li><a class="search-in-mesh-link dropdown-block-link" ref="linksrc=search_in_mesh_link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/mesh?term=%22Biomarkers%22" data-ga-category="search_mesh" data-ga-action="substance_link" data-ga-label="Biomarkers"> Search in MeSH </a></li><li><a class="add-to-search-link dropdown-block-link" ref="linksrc=add_to_search_link" role="button" data-search-term=""Biomarkers"[nm]" data-ga-category="search" data-ga-action="add_to_search" data-ga-label=""Biomarkers"[nm]" href="#"> Add to Search </a></li></ul></div></div></div></li><li><div class="keyword-actions dropdown-block"><button class="keyword-actions-trigger trigger keyword-link" aria-label="Toggle dropdown menu for keyword Coloring Agents" tabindex="0" aria-controls="keyword-actions-substances-1" aria-expanded="false" data-pinger-ignore> Coloring Agents </button><div id="keyword-actions-substances-1" class="keyword-actions-dropdown dropdown dropdown-container" hidden aria-label="Dropdown menu for keyword Coloring Agents"><div class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=%22Coloring+Agents%22%5Bnm%5D&sort=date&sort_order=desc" data-href="/?term=%22Coloring+Agents%22%5Bnm%5D&sort=date&sort_order=desc" ref="linksrc=search_in_pubmed_keyword_link&keyword="Coloring Agents"[nm]" data-ga-category="search" data-ga-action="substance" data-ga-label="Coloring Agents"> Search in PubMed </a></li><li><a class="search-in-mesh-link dropdown-block-link" ref="linksrc=search_in_mesh_link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/mesh?term=%22Coloring+Agents%22" data-ga-category="search_mesh" data-ga-action="substance_link" data-ga-label="Coloring Agents"> Search in MeSH </a></li><li><a class="add-to-search-link dropdown-block-link" ref="linksrc=add_to_search_link" role="button" data-search-term=""Coloring Agents"[nm]" data-ga-category="search" data-ga-action="add_to_search" data-ga-label=""Coloring Agents"[nm]" href="#"> Add to Search </a></li></ul></div></div></div></li><li><div class="keyword-actions dropdown-block"><button class="keyword-actions-trigger trigger keyword-link" aria-label="Toggle dropdown menu for keyword HLA Antigens" tabindex="0" aria-controls="keyword-actions-substances-2" aria-expanded="false" data-pinger-ignore> HLA Antigens </button><div id="keyword-actions-substances-2" class="keyword-actions-dropdown dropdown dropdown-container" hidden aria-label="Dropdown menu for keyword HLA Antigens"><div class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=%22HLA+Antigens%22%5Bnm%5D&sort=date&sort_order=desc" data-href="/?term=%22HLA+Antigens%22%5Bnm%5D&sort=date&sort_order=desc" ref="linksrc=search_in_pubmed_keyword_link&keyword="HLA Antigens"[nm]" data-ga-category="search" data-ga-action="substance" data-ga-label="HLA Antigens"> Search in PubMed </a></li><li><a class="search-in-mesh-link dropdown-block-link" ref="linksrc=search_in_mesh_link" href="https://web.archive.org/web/20240427130614/https://www.ncbi.nlm.nih.gov/mesh?term=%22HLA+Antigens%22" data-ga-category="search_mesh" data-ga-action="substance_link" data-ga-label="HLA Antigens"> Search in MeSH </a></li><li><a class="add-to-search-link dropdown-block-link" ref="linksrc=add_to_search_link" role="button" data-search-term=""HLA Antigens"[nm]" data-ga-category="search" data-ga-action="add_to_search" data-ga-label=""HLA Antigens"[nm]" href="#"> Add to Search </a></li></ul></div></div></div></li><li><div class="keyword-actions dropdown-block"><button class="keyword-actions-trigger trigger keyword-link" aria-label="Toggle dropdown menu for keyword Peptides" tabindex="0" aria-controls="keyword-actions-substances-3" aria-expanded="false" data-pinger-ignore> Peptides </button><div id="keyword-actions-substances-3" class="keyword-actions-dropdown dropdown dropdown-container" hidden aria-label="Dropdown menu for keyword Peptides"><div class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/web/20240427130614/https://pubmed.ncbi.nlm.nih.gov/?term=%22Peptides%22%5Bnm%5D&sort=date&sort_order=desc" data-href="/?term=%22Peptides%22%5Bnm%5D&sort=date&sort_order=desc" 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A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols - PubMed