Non-Viral Gene Engineering, TcBuster

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href="/gmp-products/ruo-to-gmp" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3421">Transition to GMP </a> <a href="/gmp-products/loa-request" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13326">DMF/LOA Request</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2066">Immuno-Oncology</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-diagnostics" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Diagnostics <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-diagnostics" aria-labelledby="navbarDropdown-diagnostics"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Controls & Reagents<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/clinical-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7606">Clinical Quality Controls</a> <a href="/diagnostics/molecular-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5596">Molecular Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom 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class="sub-menu-header"> Instrument Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/certified-service-provider-program" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5446">Certified Service Provider (CSP) Program</a> <a href="/resources/instrument-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5471">Contract Services</a> <a href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup Tool</a> <a href="/resources/rnascope-citations" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/rnascope-citations">RNAscope Citations</a> <a href="/resources/simple-plex-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-plex-panel-builder">Simple Plex Panel Builder</a> <a href="/resources/simple-western-kit-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-kit-builder">Simple 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background-position: top center; background-repeat: no-repeat; background-size: cover;"> <div> <div class="paragraph paragraph--type--h1-2-2-text-to-media paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_h1"> <div><h1>Non-Viral Gene Engineering with TcBuster</h1></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h4>Non-Viral Gene Delivery for Cell and Gene Therapy</h4></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_call_to_action_global"> <a href="#form" class="btn btn-primary" type="link"> Contact an Expert </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div 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paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><strong>On This Page</strong><br> <a href="#Why Use">Why Use Non-Viral?</a> | <a href="#Comparison">TcBuster vs. Viral Systems</a> | <a href="#Mechanism">TcBuster Mechanism</a> | <a href="#Data">TcBuster Data</a> | <a href="#Partner">Partnering with Us</a> | <a href="#faq">FAQ</a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="tcbustervideo1"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>TcBuster™ Non-Viral Gene Engineering</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="bMVSnmxWARM" params="rel=0" style="background-image: url('https://img.youtube.com/vi/bMVSnmxWARM/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/bMVSnmxWARM?autoplay=1" class="lty-playbtn" title="TcBuster(TM) - A next-generation non-viral gene delivery system"> <span class="lyt-visually-hidden">TcBuster(TM) - A next-generation non-viral gene delivery system</span> </a> </lite-youtube> </div> </article> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><br><h3>The TcBuster Transposon Gene Editing Mechanism</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>TcBuster is a large capacity transposon system that enables transfer and stable integration of genes of interest (GOI). TcBuster can replace the use of retroviral and lentiviral vectors, leading to significant time savings during the development of cell therapeutics.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="#form" class="btn btn-primary" type="link"> Contact an Expert </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Why Use"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Why Use Non-Viral Vectors For Gene Engineering?</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>TcBuster is a non-viral transposon system that enables stable gene transfer into mammalian cells. It supports the rapid generation of transgenic cells and is a valuable tool for both proof-of-concept and manufacturing scale process development. Gene editing with TcBuster has widespread applications including:</p> <ul> <li><strong>Cancer immunotherapies</strong> – introduction of chimeric antigen receptors (CARs) or TCRs; inactivation of negative regulatory molecules; masking therapeutic cells from host immunity</li> <li><strong>Gene therapy</strong> – delivery of genes into host cells to correct genetic defects</li> <li><strong>Bioprocessing</strong> - significantly improve bioprocessing yields</li> <li><strong>Disease models</strong> – development of animal models carrying disease-associated molecular variants</li> <li><strong>Drug discovery</strong> – modulating expression of proteins that serve as drug targets</li> <li><strong>Diagnostic tools</strong> – upregulation of proteins for detection of infectious and non-infectious diseases</li> </ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/gene-engineering-tcbuster-525x300-v2.png" alt="Gene Engineering With TcBuster" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Comparison"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Comparing TcBuster and Virus-Based Systems</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Sidestep the hurdles of gene engineering with lentivirus systems by using a non-viral method. The use of TcBuster avoids common viral method challenges including low transduction efficiency, inconsistent reagent availability, and an insecure supply chain for GMP-grade virus.</p> <p>Virus-based gene delivery (<em>e.g.</em> with lentivirus or AAV) involves packaging of the GOI into the viral vector followed by transduction of target cells. TcBuster transposons can be quickly generated and electroporated into target cells. As shown in the graphic below, TcBuster protocols eliminate the lengthy step for viral vector packaging.</p> <p>Enhance your cell engineering process with TcBuster and:</p> <ul> <li><strong>Shorten your cell engineering timeline</strong> and reduce the risk of delays.</li> <li><strong>Integrate larger cargo sizes</strong> than with standard viral based methods. AAV can typically deliver 4-5 kb of DNA, while TcBuster can deliver >10 kb (enough for 2 TCRs plus an accessory protein).</li> <li><strong>Maintain high efficiency gene transfer</strong> and stable expression. This enables rapid generation of transgenic mammalian cells with limited secondary effects and no selection steps.</li> <li><strong>Reduce the costs</strong> of genetic modification.</li> <li><strong>Exploit the flexibility</strong> of TcBuster to carry out additional gene editing techniques in one operation.</li> <li><strong>Scale from research to clinical and commercial effortlessly </strong>with TcBuster gene editing and our partners at <strong><a href="/research-areas/immune-cell-therapy/scaleready">ScaleReady</a></strong></li> </ul></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/services/non-viral-gene-delivery-v3.jpg" alt="TcBuster scales to GMP in 6 months" loading="lazy" > </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Mechanism"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Hear From Bio-Techne Scientists</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h4>Take your cell therapy beyond the payload capacity limit with a non-viral transposon system.</h4></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="goKKweUCexc" params="rel=0" style="background-image: url('https://img.youtube.com/vi/goKKweUCexc/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/goKKweUCexc?autoplay=1" class="lty-playbtn" title="Hear From Our Scientists - What Is TcBuster?"> <span class="lyt-visually-hidden">Hear From Our Scientists - What Is TcBuster?</span> </a> </lite-youtube> </div> </article> </div> </div> <div class="media-caption"> <div>Watch this video to hear from our experts about gene engineering with TcBuster.</div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Realize the potential of gene engineering by using TcBuster to deliver large genes of interest. Gene editing is a powerful tool for fine tuning cell phenotype in immune cell and regenerative medicine therapies, and it can augment therapeutic effectiveness by increasing cell potency and persistence <em>in vivo</em>.</p> <p>Partner with our experts to engineer T cells, NK cells, feeder cells, and induced pluripotent stem cells (iPSC) for your cell therapy program.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="#form" class="btn btn-primary" type="link"> Inquire About TcBuster for Gene Engineering </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Data"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div><h3>Transfection Efficiency with Non-Viral Gene Delivery</h3></div> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/tc-buster-data-banner-v2.png" alt="(Left) Transfection efficiency of T cells using TcBuster for CD19 CAR, (Right) Killing efficiency of CAR-T cells generated with TcBuster" loading="lazy" > </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Similar CAR expression was achieved in primary human T cells edited with a CD19 CAR using TcBuster or lentivirus as shown by detection with the <strong><a href="/search?keywords=cd19&category_attr=Proteins%20and%20Enzymes">Fluorokine fluorescent CD19 antigen</a></strong> (left 2 panels). In the right panel, CD19 CAR-T cells engineered using TcBuster (dark blue) or lentivirus (light blue) show similar cytotoxicity against CD19<sup>+</sup> Nalm6/luc cells. The non-electroporated controls (gray) do not kill target cells. </p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/tcbuster-data-525x300.png" alt="Delivery of large CAR constructs with TcBuster" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>Transposition Capacity With TcBuster</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>TcBuster can efficiently deliver both small single CAR constructs and large-sized, multi-cistronic constructs. Activated T cells were transposed with increasing plasmid sizes, expanded for 4 days, and analyzed for CAR expression by flow cytometry. >30% transposition efficiency was achieved with constructs ranging up to 6 kb of insert. </p></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Partner"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <a href="https://www.bio-techne.com/research-areas/immune-cell-therapy/scaleready" title="Learn More About ScaleReady" target="_blank" rel="noreferrer" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/scaleready-logo-525x233.png" alt="ScaleReady Logo" loading="lazy" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Bio-Techne believes in the power of partnerships. We have joined with Fresenius Kabi and Wilson Wolf to bring you ScaleReady™, lowering the barriers to entry into the immune cell therapy space for all sizes and stages of manufacturing programs. ScaleReady brings cell and gene therapies to life by offering a significant reduction of complexity and cost while consistently providing superior repeatability and cell quality.</p> <p>Partner with <strong><a href="https://www.scaleready.com/?utm_source=bio-techne.com&utm_medium=referral&utm_campaign=cgt&utm_term=cgt" target="_blank">ScaleReady</a></strong> for TcBuster applications in immune cell therapy. For other applications of TcBuster, contact a <strong><a href="#form">gene engineering expert at Bio-Techne</a></strong>.</p></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/research-areas/immune-cell-therapy/scaleready" class="btn btn-primary" type="link"> Learn More About ScaleReady </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://www.luminarytx.com/bio-techne-luminary-therapeutics-sign-license-agreement-for-the-development-car-t-cell-therapies/" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/luminary-therapeutics-logo-v2.png" alt="Luminary Therapeutics Corporate Logo" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://www.luminarytx.com/bio-techne-luminary-therapeutics-sign-license-agreement-for-the-development-car-t-cell-therapies/" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p>Luminary Therapeutics has signed a licence agreement for the use of TcBuster for the manufacturing of gene-modified cell therapies. Luminary Therapeutics will use TcBuster in their LMY-920 program, a BAFF-CAR-T product.</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://www.luminarytx.com/bio-techne-luminary-therapeutics-sign-license-agreement-for-the-development-car-t-cell-therapies/" > Learn More at Luminary Therapeutics </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/c-bio-204wt-1018609-cgti2022152-v5.pdf" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/cgt-insights-tcbuster-article-thumbnail-500x580.png" alt="Cell & Gene Therapy article on TcBuster" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://resources.bio-techne.com/bio-techne-assets/images/literature/c-bio-204wt-1018609-cgti2022152-v5.pdf" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p>This article in Cell & Gene Therapy Insights discusses the transposon-based TcBuster platform and its competitive advantages in cell and gene therapy. Directed evolution of hyperactive TcBuster, TcBuster in cell therapies, advantages and applications of TcBuster, Q&A with the authors.</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/c-bio-204wt-1018609-cgti2022152-v5.pdf" > Read TcBuster Article </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/t-cell-therapies-ebook" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/t-cell-based-therapies-ebook-thumbnail-500x580.png" alt="T Cell-Based Therapies eBook" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/t-cell-therapies-ebook" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p>This eBook provides an overview of several biological and manufacturing challenges facing the development and production of T cell therapies. It follows with examples of how Bio-Techne products and services can address these challenges at each stage of the process.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/t-cell-therapies-ebook" > Download T Cell Therapies eBook </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="faq"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h3>Frequently Asked Questions</h3></div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-can-transposons-or-lentivirus-be-used-for-in-vivo-gene-delivery3" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Can transposons or lentivirus be used for in vivo gene delivery?</h5> </a> </div> <div class="collapse" id="expPara-can-transposons-or-lentivirus-be-used-for-in-vivo-gene-delivery3" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Theoretically transposons can be used for <em>in vivo</em> delivery and is an active area of research in the field. Current delivery mechanisms don’t allow for efficient enough transfer and editing efficiency.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-what-is-dna-toxicity-from-a-transposase-or-virus2" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What is DNA toxicity from a transposase or virus?</h5> </a> </div> <div class="collapse" id="expPara-what-is-dna-toxicity-from-a-transposase-or-virus2" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Foreign DNA from the transposon can activate intracellular <strong><a href="https://www.rndsystems.com/pathways/rig-i-like-receptor-signaling-pathways" target="_blank">DNA sensing mechanisms</a></strong> and trigger <strong><a href="/t/apoptosis-detection/activity-assays" target="_self">apoptosis</a></strong>. The toxicity can be managed with specific cell handling techniques during transfection. One reason for delivering the transposase as mRNA is to avoid triggering these responses.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-why-are-transposons-delivered-as-dna-or-rna-instead-of-as-protein20" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Why are transposons delivered as DNA or RNA instead of as protein?</h5> </a> </div> <div class="collapse" id="expPara-why-are-transposons-delivered-as-dna-or-rna-instead-of-as-protein20" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>The transposon must be delivered as a DNA template as this will be directly inserted into the genome. The transposase is delivered as mRNA to prevent any possible random integration of the enzyme and decrease <strong><a href="http://www.rndsystems.com/pathways/rig-i-like-receptor-signaling-pathways" target="_blank">DNA mediated toxicity</a></strong>. Transposases are difficult to manufacture as purified protein in high enough concentrations for transfection due to their relatively insoluble nature.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-whats-the-definition-of-stable-gene-transfer13" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What’s the definition of stable gene transfer?</h5> </a> </div> <div class="collapse" id="expPara-whats-the-definition-of-stable-gene-transfer13" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Gene transfer is considered stable if the DNA is integrated into the cellular genome. Once incorporated, we have not observed any issues with silencing or loss of expression. In transformed cell lines we routinely use a selection cassette as part of the transposon to allow for re-selection of the cells if necessary. In T cells we have performed repeated stimulations of transposed cells and do not observe loss of transgene expression over time.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-can-viruses-deliver-multiple-genes-if-theyre-small-enough-is-multiple-gene-delivery-unique-to-transposons18" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Can viruses deliver multiple genes if they’re small enough? Is multiple gene delivery unique to transposons?</h5> </a> </div> <div class="collapse" id="expPara-can-viruses-deliver-multiple-genes-if-theyre-small-enough-is-multiple-gene-delivery-unique-to-transposons18" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Multicistronic vectors can be used with viruses, although due to packaging limitations in viruses the yield and titer of the virus are often negatively impacted when delivering multiple genes. Transposons can move larger cargo and are therefore better suited to multicistronic vectors.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-whats-hyperactive-about-tcbuster23" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What’s hyperactive about TcBuster?</h5> </a> </div> <div class="collapse" id="expPara-whats-hyperactive-about-tcbuster23" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>TcBuster has been engineered to have increased enzymatic activity over the wildtype version of the enzyme. This increases the efficiency of gene transfer to rates that are equal to or better than alternate gene engineering systems.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-why-is-gene-transposition-into-t-cells-more-efficient-on-resting-cells-than-activated-cells24" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Why is gene transposition into T cells more efficient on resting cells than activated cells?</h5> </a> </div> <div class="collapse" id="expPara-why-is-gene-transposition-into-t-cells-more-efficient-on-resting-cells-than-activated-cells24" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Resting cells have a relatively larger nucleus, making it easier to get DNA into the nucleus. Proliferating cells have enlarged cytoplasm, so the nucleus is a relatively smaller target.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-do-t-cells-have-to-be-pre-activated-for-transposition-to-work5" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Do T cells have to be pre-activated for transposition to work?</h5> </a> </div> <div class="collapse" id="expPara-do-t-cells-have-to-be-pre-activated-for-transposition-to-work5" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>No, TcBuster can work with both resting or pre-activated T cells.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-why-doesnt-random-insertion-have-a-greater-chance-of-disrupting-important-gene-transcription2" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Why doesn’t random insertion have a greater chance of disrupting important gene transcription?</h5> </a> </div> <div class="collapse" id="expPara-why-doesnt-random-insertion-have-a-greater-chance-of-disrupting-important-gene-transcription2" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>The gene insertion profile of TcBuster has been studied and has been found to be more random than that of lentivirus. While viruses do “randomly” integrate they preferentially insert into more active regions, i.e gene-coding regions. TcBuster-M and transposons in general are more random in their insertional profile decreasing the likelihood of disrupting gene transcription as there is less coding genome than non-coding.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-does-package-size-affect-integration-efficiency-for-tcbuster24" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Does package size affect integration efficiency for TcBuster?</h5> </a> </div> <div class="collapse" id="expPara-does-package-size-affect-integration-efficiency-for-tcbuster24" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>TcBuster can consistently integrate a single CAR into primary CD4+/CD8+ T-cells with an efficiency of 50-70% depending on the exact construct. As construct size and complexity increase, the transposition rates do tend to decline modestly. Transposition of multicistronic vectors encoding either 3 or 4 transgenes have achieved rates from 25-40%.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-what-other-applications-can-tcbuster-be-used-for8" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What other applications can TcBuster be used for?</h5> </a> </div> <div class="collapse" id="expPara-what-other-applications-can-tcbuster-be-used-for8" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>TcBuster can be used in cell therapy for gene modification of <strong><a href="/research-areas/immune-cell-therapy/t-cell-manufacturing" target="_self">T cells</a></strong>, <strong><a href="/research-areas/immune-cell-therapy/nk-cells" target="_self">NK cells</a></strong>, and <strong><a href="/research-areas/stem-cells/ipsc" target="_self">iPSCs</a></strong>. In addition, it can be used to generate stable producer cell lines for either antibody or protein production, and it is used to over express genes in cell lines for drug discovery or screening purposes.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-additional-resources7" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Additional Resources</h5> </a> </div> <div class="collapse" id="expPara-additional-resources7" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p><a href="/reagents/cell-culture-reagents/excellerate">ExCellerate Serum-Free and Animal-Free Cell Expansion Media</a></p> <p><a href="/research-areas/cell-and-gene-therapy/analytical-solutions">Cell and Gene Therapy Analytical Solutions</a></p> <p><a href="/gmp-products/gmp-proteins">GMP Proteins</a></p> <p><a href="/gmp-products/gmp-proteins/sample-gmp-proteins">Request a GMP Protein Sample</a></p> <p><a href="/gmp-products/gmp-small-molecules">GMP Small Molecules</a></p> <p><a href="/gmp-products/ruo-to-gmp">Streamlining Transition to GMP</a></p> <p><a href="/reagents/cell-culture-reagents/serum-free-animal-free">Serum-Free and Animal-Free Cell Culture</a></p> <p><a href="https://www.tocris.com/product-type/viral-transduction-enhancers?_ga=2.210050773.1558343718.1649071008-1301604656.1637169657" target="_blank">Viral Transduction Enhancers</a> at Tocris</p> <p><a href="https://acdbio.com/science/applications/research-areas/gene-therapyaav?_ga=2.219971545.1558343718.1649071008-1301604656.1637169657" target="_blank">ISH Assays for AAV Biodistribution</a> at ACD</p> <p><a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/cell-kill-appnote.pdf" target="_blank">Quantify Secreted T Cell Activation Markers in 90 Minutes</a></p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-video-transcription-hear-from-our-scientists-what-is-tcbuster3" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Video Transcription: Hear From Our Scientists - What Is TcBuster?</h5> </a> </div> <div class="collapse" id="expPara-video-transcription-hear-from-our-scientists-what-is-tcbuster3" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>TcBuster is our proprietary transpose transposon genome engineering technology. Transposons are a virus-free way to over-express a particular protein in a cell line. Like the other systems it's a cut-and-paste transposon so you deliver your gene of interest (GOI) within a plasmid that's flanked by sequences that TcBuster recognizes. It cuts out your GOI and randomly inserts it into the genome. TcBuster is a virus-free way to overexpress pretty much any protein of interest in a cell.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-video-transcription-tcbuster-a-next-generation-non-viral-gene-delivery-system18" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Video Transcription: TcBuster™ - A next-generation non-viral gene delivery system</h5> </a> </div> <div class="collapse" id="expPara-video-transcription-tcbuster-a-next-generation-non-viral-gene-delivery-system18" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Genome engineering unleashes new abilities to alter cells and advanced cell therapies. But even the most cutting-edge tools such as CRISPR-Cas9 and lentivirus have their limitations. To realize the full power of genome engineering, Bio-Techne developed a non-viral transposon gene delivery system, called TcBuster. Here's how it works. The TcBuster system consists of a plasmid containing the cargo sequence flanked on each side by inverted tandem repeats (ITRs), plus mRNA encoding the TcBuster transposase. Both components are delivered into the cells using standard transfection. Once inside the cell, the expressed transposase binds the ITRs, cuts out your sequence, and inserts it safely and randomly into the host genome. Whether you are generating cell lines, reprogramming iPSCs, or creating the next breakthrough CAR therapies, with TcBuster you can eliminate high cost, mutagenic potential, and cargo size constraints of virus-based methods for faster, more complex and potentially safer gene delivery. To benefit scientists everywhere from research to commercial scale, TcBuster is non-exclusively licensed. Learn how TcBuster can work for you by completing a project request form, and our team will contact you.</p></div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="form"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h4>Tell us about your project, and one of our experts will contact you to discuss how we can help.</h4></div> </div> </div> </div> <div> <div id="block-bio-techne-global-content" class="paragraph"> <article> <div class="row"> <div class="col-8 offset-md-2 offset-sm-0"> <script src="//info.bio-techne.com/js/forms2/js/forms2.min.js"></script> <form id="mktoForm_6120"></form> <script>MktoForms2.loadForm("//info.bio-techne.com", "584-XSF-168", 6120);</script> </div> </div> </article> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide 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