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et_pb_with_background et_section_regular" > <span class="et_parallax_bg_wrap"><span class="et_parallax_bg" style="background-image: url(https://pathoquest.com/wp-content/uploads/2023/08/NCATS-banner.png);" ></span></span> <div class="et_pb_row et_pb_row_0 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_0 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_image et_pb_image_0"> <span class="et_pb_image_wrap "><img fetchpriority="high" decoding="async" width="626" height="522" src="https://pathoquest.com/wp-content/uploads/2023/08/Services-NCATS-Intergration-Site-Analysis.png" alt="" title="Services-NCATS-Intergration-Site-Analysis" srcset="https://pathoquest.com/wp-content/uploads/2023/08/Services-NCATS-Intergration-Site-Analysis.png 626w, https://pathoquest.com/wp-content/uploads/2023/08/Services-NCATS-Intergration-Site-Analysis-480x400.png 480w" sizes="(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) 626px, 100vw" class="wp-image-707" /></span> </div><div class="et_pb_module et_pb_text et_pb_text_0 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h1 style="text-align: center;">Integration Site Analysis</h1> <p style="text-align: center;">Characterisation of genetic modifications for clone selection, genetic stability and lot release.</p> <p style="text-align: center;">The first GMP assay using Oxford Nanopore’s long-read sequencing technology.</p></div> </div><div class="et_pb_module et_pb_image et_pb_image_1"> <span class="et_pb_image_wrap "><img decoding="async" width="3369" height="2382" src="https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-INTEGRATION-SITE-ANALYSIS-HR.png" alt="" title="iDTECT INTEGRATION SITE ANALYSIS- HR" srcset="https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-INTEGRATION-SITE-ANALYSIS-HR.png 3369w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-INTEGRATION-SITE-ANALYSIS-HR-1280x905.png 1280w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-INTEGRATION-SITE-ANALYSIS-HR-980x693.png 980w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-INTEGRATION-SITE-ANALYSIS-HR-480x339.png 480w" sizes="(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 3369px, 100vw" class="wp-image-1225" /></span> </div> </div> </div> </div><div class="et_pb_section et_pb_section_1 et_pb_with_background et_section_regular" > <div class="et_pb_row et_pb_row_1 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_1 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_1 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: center;">What is INTEGRATION SITE ANALYSIS and why is it important?</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_0 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div> </div> </div><div class="et_pb_row et_pb_row_2 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_1_2 et_pb_column_2 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_2 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p>As part of the development of a cell line for manufacturing, candidate clones will be selected based on several attributes including production yield, number of transgene copies, as well as the structure and location of the transgene in the host cell genome. Indeed, a certain level of genetic characterization of the cell line is expected by the regulatory authorities (as outlined in <a href="https://pathoquest.com/services/cell-line-characterization/"><strong>Cell Line Characterization</strong></a>).</p> <p>However, developers can benefit from a deeper understanding of the genome integration site of the transgene when making clone selection. For example, transgenes located in areas of instability or regions prone to epigenetic silencing may lead to a significant reduction in manufacturing yields over the cell line’s manufacturing lifetime.</p> <p>Employing long read sequencing techniques, such as nanopore long read sequencing, can provide detailed insights into the genetic site of transgene integration. This genome sequence analysis allows for the identification of potential instability or silencing issues that might not be detected with shorter read methods. By using long read sequencing, developers can ensure that transgenes are integrated into stable regions of the genome, thereby optimizing production yields, and maintaining consistency throughout the cell line’s manufacturing lifecycle.</p></div> </div> </div><div class="et_pb_column et_pb_column_1_2 et_pb_column_3 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_3 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p>Classical integration site analysis methods such as fluorescence in situ hybridization (FISH) or Southern blotting can provide information on the integration location in the genome but still offer very low resolution. Sequencing the integration site offers single nucleotide resolution; however, it can be challenging, especially if the transgene is randomly integrated into the host genome. Methods such as long-range PCR combined with Sanger sequencing are also employed, but these are cumbersome and require prior knowledge of the integration site. More recently, NGS whole genome sequencing (WGS) has been used to characterize the integration site. However, WGS can be complex for cells with unstable genomes such as CHO. Additionally, WGS tends to provide a lower level of sequence coverage, making conclusive identification of the integration site and its epigenetic status difficult.</p> <p>A targeted short-read NGS method utilizes whole genome crosslinking to generate circular DNA fragments up to 100kb either side of a known primer site from the gene of interest. This method does not require any prior sequence information of the insertion site, and good information is obtained on the junction site. However, due to the nature of short-read sequencing, this approach can struggle to identify concatemers, truncations, and inverted repeats.</p></div> </div> </div> </div><div class="et_pb_row et_pb_row_3 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_4 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_4 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p>With iDTECT® INTEGRATION SITE ANALYSIS, PathoQuest has validated an enhanced integration site analysis approach that combines long read nanopore sequencing with transgene expression cassette-targeted cleavage, called long-read ISA. By targeting cleavage to the transgene expression cassette, both the transgene and the junction site can be comprehensively sequenced. Long read sequencing has the advantage over short-read NGS methods as it better characterizes concatemers, truncations, and inverted repeats. This method provides detailed insights into the genetic site of transgene integration, allowing for comprehensive genome sequence analysis and a more accurate understanding of genome integration.</p></div> </div> </div> </div><div class="et_pb_row et_pb_row_4 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_1_6 et_pb_column_5 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_0_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_0 et_pb_bg_layout_light" href="#WWD">WHAT WE DO</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_6 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_1_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_1 et_pb_bg_layout_light" href="#WTA">WHY THIS APPROACH</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_7 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_2_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_2 et_pb_bg_layout_light" href="#SR">SAMPLE REQUIREMENTS</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_8 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_3_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_3 et_pb_bg_layout_light" href="#CS">CHALLENGES SOLVED</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_9 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_4_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_4 et_pb_bg_layout_light" href="#OS">OTHER SERVICES</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_10 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_button_module_wrapper et_pb_button_5_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_5 et_pb_bg_layout_light" href="#contact">CONTACT US</a> </div> </div> </div> </div><div id="WWD" class="et_pb_section et_pb_section_2 et_pb_with_background et_pb_section_parallax et_section_regular" > <div class="et_pb_row et_pb_row_5 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_1_2 et_pb_column_11 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_5 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2>What we do</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_1 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_6 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><b>Modalities Tested:</b></p> <ul> <li><span style="color: #2e3180;">Cell banks for mAbs and recombinant proteins biologics, Viral vectors, Cell therapies, Vaccines</span></li> <li><span style="color: #2e3180;">Cell therapy lots</span></li> <li><span style="color: #2e3180;">Gene therapy</span></li> <li><span style="color: #2e3180;">Cultured meat banks</span></li> </ul> <p><b>Benefits of Long-Read iDTECT<sup>®</sup> NGS assay</b></p> <p><span style="color: #f6a800;"><code class="hljs language-css"><span class="hljs-string">✓ </span></code></span>GMP validated</p> <p><span><span style="color: #f6a800;"><code class="hljs language-css"><span class="hljs-string">✓</span></code></span> Resolve concatemers, truncations and inverted repeats more easily than short-read NGS</span></p> <p><strong><span>Our Assay:</span></strong></p> <ul> <li><span style="color: #2e3180;">iDTECT<sup>®</sup> Integration Site Analysis</span></li> </ul></div> </div> </div><div class="et_pb_column et_pb_column_1_2 et_pb_column_12 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_7 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: center;">INTEGRATION SITE ANALYSIS METHODS</h2></div> </div><div class="et_pb_module et_pb_image et_pb_image_2"> <span class="et_pb_image_wrap "><img loading="lazy" decoding="async" width="1191" height="650" src="https://pathoquest.com/wp-content/uploads/2024/07/infographic-1.png" alt="" title="infographic" srcset="https://pathoquest.com/wp-content/uploads/2024/07/infographic-1.png 1191w, https://pathoquest.com/wp-content/uploads/2024/07/infographic-1-980x535.png 980w, https://pathoquest.com/wp-content/uploads/2024/07/infographic-1-480x262.png 480w" sizes="(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) 1191px, 100vw" class="wp-image-1712" /></span> </div> </div> </div> </div><div id="WTA" class="et_pb_section et_pb_section_3 et_pb_with_background et_pb_section_parallax et_section_regular" > <div class="et_pb_row et_pb_row_6 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_13 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_8 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: center;">WHY THIS APPROACH</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_2 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div> </div> </div><div class="et_pb_row et_pb_row_7 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_with_border et_pb_column_1_4 et_pb_column et_pb_column_14 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_9 et_clickable et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: center;"><b>ICH: </b>Scientific guideline Q5B analysis of the expression construct in cell lines</p> <p style="text-align: center;"><a https:="" www="" ema="" europa="" eu="" en="" documents="" scientific-guideline="" ich-q-7-good-manufacturing-practice-active-pharmaceutical-ingredients-step-5_en="" pdf="" _blank="" href="https://www.ema.europa.eu/en/ich-q5b-analysis-expression-construct-cell-lines-used-production-rdna-derived-protein-products" target="_blank" rel="noopener" ich-q-7-good-manufacturing-practice-active-pharmaceutical-ingredients-step-5_><strong><span style="color: #249fda;">FIND OUT MORE</span></strong></a></p></div> </div> </div><div class="et_pb_with_border et_pb_column_1_4 et_pb_column et_pb_column_15 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_10 et_clickable et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: center;"><b>FDA:</b> Characterization and Qualification of Cell Substrates and Other Biological Materials Used in the Production of Viral Vaccines for Infectious Disease Indications</p> <p style="text-align: center;"><a href="https://www.fda.gov/media/78428/download" target="_blank" rel="noopener"><strong><span style="color: #249fda;">FIND OUT MORE</span></strong></a></p></div> </div> </div><div class="et_pb_with_border et_pb_column_1_4 et_pb_column et_pb_column_16 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_11 et_clickable et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: center;"><b><strong>Publication</strong>:</b> Nanopore Cas9‐targeted sequencing enables accurate and simultaneous identification of transgene integration sites, their structure and epigenetic status.</p> <p style="text-align: center;"><a href="https://doi.org/10.1002/bit.28382" target="_blank" rel="noopener"><strong><span style="color: #249fda;">FIND OUT MORE</span></strong></a></p></div> </div> </div><div class="et_pb_with_border et_pb_column_1_4 et_pb_column et_pb_column_17 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_12 et_clickable et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: center;">PathoQuest Webinar</p> <p style="text-align: center;"><a href="https://vimeo.com/879842460" target="_blank" rel="noopener"><strong><span style="color: #249fda;">FIND OUT MORE</span></strong></a></p></div> </div> </div> </div> </div><div id="SR" class="et_pb_section et_pb_section_4 et_pb_section_parallax et_pb_with_background et_section_regular" > <span class="et_parallax_bg_wrap"><span class="et_parallax_bg" style="background-image: url(https://pathoquest.com/wp-content/uploads/2023/06/Swoosh_background.svg);" ></span></span> <div class="et_pb_row et_pb_row_8 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_18 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_13 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: left;">SAMPLE REQUIREMENTS</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_3 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_14 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p>The specific sample requirements can depend on the source material and the desired outputs. We would therefore recommend having a consultation with one of our experts to discuss your exact requirements.</p> <p>In general our sample requirements are:</p> <ul> <li><span style="color: #2e3180;"><span style="color: #2e3180;">5×10⁶ </span></span><span style="color: #2e3180;"><span style="color: #2e3180;">cells recommended (≥10µg DNA per sample</span></span><span style="color: #2e3180;">)</span></li> <li><span style="color: #2e3180;">Storage and shipment at -80°C or on dry ice</span></li> <li><span style="color: #2e3180;">Cell pellets or cryo-preservation buffer</span></li> <li><span style="color: #2e3180;">Back up sample is recommended</span></li> </ul></div> </div> </div> </div> </div><div id="CS" class="et_pb_section et_pb_section_5 et_pb_section_parallax et_pb_with_background et_section_regular" > <span class="et_parallax_bg_wrap"><span class="et_parallax_bg" style="background-image: url(https://pathoquest.com/wp-content/uploads/2023/06/dentity_confirmation_mid_banner.webp);" ></span></span> <div class="et_pb_row et_pb_row_9 et_pb_equal_columns"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_19 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_15 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2>Challenges solved</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_4 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div> </div> </div><div class="et_pb_row et_pb_row_10 et_pb_equal_columns"> <div class="et_pb_column et_pb_column_1_2 et_pb_column_20 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_16 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><ul> <li><span style="color: #ffffff;">Understanding complex transgene arrangements</span></li> <li><span style="color: #ffffff;">Low sample volumes</span></li> <li><span style="color: #ffffff;">Tight clinical timelines</span></li> <li><span style="color: #ffffff;">Reducing risk in cell banking with more information on integration site</span></li> </ul></div> </div> </div><div class="et_pb_column et_pb_column_1_2 et_pb_column_21 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_slider et_pb_slider_0 et_pb_slider_fullwidth_off"> <div class="et_pb_slides"> <div class="et_pb_slide et_pb_slide_0 et_pb_bg_layout_dark et_pb_media_alignment_center et-pb-active-slide" data-slide-id="et_pb_slide_0"> <div class="et_pb_container clearfix"> <div class="et_pb_slider_container_inner"> <div class="et_pb_slide_description"> <div class="et_pb_slide_content"><p style="text-align: left;">“We acquired a production cell bank that had minimal information on the integration site other than the sequence of the transgene. PathoQuest’s approach allowed us to fully sequence the insertion site giving us the confidence to take our bank forward for GMP manufacturing”</p></div> </div> </div> </div> </div> </div> </div> </div> </div><div class="et_pb_row et_pb_row_11 et_pb_equal_columns et_pb_gutters1"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_22 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_button_module_wrapper et_pb_button_6_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_6 et_pb_bg_layout_light" href="#contact">CONTACT US</a> </div> </div> </div> </div><div id="OS" class="et_pb_section et_pb_section_6 et_section_regular" > <div class="et_pb_row et_pb_row_12 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_23 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_17 et_pb_text_align_left et_pb_bg_layout_light"> 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function() {gformInitSpinner( 1, 'https://pathoquest.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery('#gform_ajax_frame_1').on('load',function(){var contents = jQuery(this).contents().find('*').html();var is_postback = contents.indexOf('GF_AJAX_POSTBACK') >= 0;if(!is_postback){return;}var form_content = jQuery(this).contents().find('#gform_wrapper_1');var is_confirmation = jQuery(this).contents().find('#gform_confirmation_wrapper_1').length > 0;var is_redirect = contents.indexOf('gformRedirect(){') >= 0;var is_form = form_content.length > 0 && ! is_redirect && ! is_confirmation;var mt = parseInt(jQuery('html').css('margin-top'), 10) + parseInt(jQuery('body').css('margin-top'), 10) + 100;if(is_form){jQuery('#gform_wrapper_1').html(form_content.html());if(form_content.hasClass('gform_validation_error')){jQuery('#gform_wrapper_1').addClass('gform_validation_error');} else {jQuery('#gform_wrapper_1').removeClass('gform_validation_error');}setTimeout( function() { /* delay the scroll by 50 milliseconds to fix a bug in chrome */ jQuery(document).scrollTop(jQuery('#gform_wrapper_1').offset().top - mt); }, 50 );if(window['gformInitDatepicker']) {gformInitDatepicker();}if(window['gformInitPriceFields']) {gformInitPriceFields();}var current_page = jQuery('#gform_source_page_number_1').val();gformInitSpinner( 1, 'https://pathoquest.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery(document).trigger('gform_page_loaded', [1, current_page]);window['gf_submitting_1'] = false;}else if(!is_redirect){var confirmation_content = jQuery(this).contents().find('.GF_AJAX_POSTBACK').html();if(!confirmation_content){confirmation_content = contents;}jQuery('#gform_wrapper_1').replaceWith(confirmation_content);jQuery(document).scrollTop(jQuery('#gf_1').offset().top - mt);jQuery(document).trigger('gform_confirmation_loaded', [1]);window['gf_submitting_1'] = false;wp.a11y.speak(jQuery('#gform_confirmation_message_1').text());}else{jQuery('#gform_1').append(contents);if(window['gformRedirect']) {gformRedirect();}}jQuery(document).trigger("gform_pre_post_render", [{ formId: "1", currentPage: "current_page", abort: function() { this.preventDefault(); } }]); if (event && event.defaultPrevented) { return; } const gformWrapperDiv = document.getElementById( "gform_wrapper_1" ); if ( gformWrapperDiv ) { const visibilitySpan = document.createElement( "span" ); visibilitySpan.id = "gform_visibility_test_1"; gformWrapperDiv.insertAdjacentElement( "afterend", visibilitySpan ); } const visibilityTestDiv = document.getElementById( "gform_visibility_test_1" ); let postRenderFired = false; function triggerPostRender() { if ( postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [1, current_page] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 1, currentPage: current_page } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); }; var callNow = immediate && !timeout; clearTimeout( timeout ); timeout = setTimeout( later, wait ); if ( callNow ) func.apply( context, args ); }; } const debouncedTriggerPostRender = debounce( function() { triggerPostRender(); }, 200 ); if ( visibilityTestDiv && visibilityTestDiv.offsetParent === null ) { const observer = new MutationObserver( ( mutations ) => { mutations.forEach( ( mutation ) => { if ( mutation.type === 'attributes' && visibilityTestDiv.offsetParent !== null ) { debouncedTriggerPostRender(); observer.disconnect(); } }); }); observer.observe( document.body, { attributes: true, childList: false, subtree: true, attributeFilter: [ 'style', 'class' ], }); } else { triggerPostRender(); } } );} ); /* ]]> */ </script> </section> </div> </div> </div> </div><div class="et_pb_row et_pb_row_17 et_pb_equal_columns et_pb_gutters1"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_35 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_31 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2><span style="color: #ffffff;"><span class="ui-provider bra brb c d e f g h i j k l m n o p q r s t brc brd w x y z ab ac ae af ag ah ai aj ak" dir="ltr">U.S.</span></span></h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_8 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_32 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><span class="ui-provider bra brb c d e f g h i j k l m n o p q r s t brc brd w x y z ab ac ae af ag ah ai aj ak" dir="ltr" style="color: #ffffff;"><a rel="noreferrer noopener" title="tel:+14842129360" target="_blank" style="color: #ffffff;">+1 484 212 9360</a></span></p> <p><span style="color: #ffffff;">466 Devon Park Dr<br />Wayne, PA 19087<br />United States</span></p></div> </div><div class="et_pb_module et_pb_text et_pb_text_33 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2><span style="color: #ffffff;"><span class="ui-provider bra brb c d e f g h i j k l m n o p q r s t brc brd w x y z ab ac ae af ag ah ai aj ak" dir="ltr">France</span></span></h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_9 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_34 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><span style="color: #ffffff;">+33 (0)1 70 82 17 90</span></p> <p><span style="color: #ffffff;">Biopark -Bâtiment B,<br />11, rue Watt<br />75013 Paris, France</span></p></div> </div><div class="et_pb_module et_pb_text et_pb_text_35 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><strong>How can PathoQuest help?</strong></p></div> </div><div class="et_pb_module et_pb_wpt_gravityform et_pb_wpt_gravityform_1"> <div class="et_pb_module_inner"> <section class='et_pb_module et_pb_wpt_gravityform et_pb_wpt_gravityform_1' data-class='et_pb_module et_pb_wpt_gravityform et_pb_wpt_gravityform_1' id="" data-type='et_pb_wpt_gravityform' data-is-sticky='no'> <div class='gf_browser_chrome gform_wrapper gravity-theme gform-theme--no-framework' data-form-theme='gravity-theme' data-form-index='1' id='gform_wrapper_1' ><form method='post' enctype='multipart/form-data' id='gform_1' action='/services/integration-site-analysis/' data-formid='1' novalidate> <input type='hidden' class='gforms-pum' value='{"closepopup":false,"closedelay":0,"openpopup":false,"openpopup_id":0}' /> <div class='gform-body gform_body'><div id='gform_fields_1' class='gform_fields top_label form_sublabel_above description_above validation_below'><fieldset id="field_1_1" class="gfield gfield--type-name gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_1" ><legend class='gfield_label gform-field-label gfield_label_before_complex' >Name<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></legend><div class='ginput_complex ginput_container ginput_container--name no_prefix has_first_name no_middle_name has_last_name no_suffix gf_name_has_2 ginput_container_name gform-grid-row' id='input_1_1'> <span id='input_1_1_3_container' class='name_first gform-grid-col gform-grid-col--size-auto' > <label for='input_1_1_3' class='gform-field-label gform-field-label--type-sub '>First</label> <input type='text' name='input_1.3' id='input_1_1_3' value='' aria-required='true' autocomplete="given-name" /> </span> <span id='input_1_1_6_container' class='name_last gform-grid-col gform-grid-col--size-auto' > <label for='input_1_1_6' class='gform-field-label gform-field-label--type-sub '>Last</label> <input type='text' name='input_1.6' id='input_1_1_6' value='' aria-required='true' autocomplete="family-name" /> </span> </div></fieldset><div id="field_1_2" class="gfield gfield--type-email gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_2" ><label class='gfield_label gform-field-label' for='input_1_2'>Email<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_email'> <input name='input_2' id='input_1_2' type='email' value='' class='large' aria-required="true" aria-invalid="false" /> </div></div><div id="field_1_4" class="gfield gfield--type-text gfield--width-full gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_4" ><label class='gfield_label gform-field-label' for='input_1_4'>Company<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_text'><input name='input_4' id='input_1_4' type='text' value='' class='large' aria-required="true" aria-invalid="false" /> </div></div><div id="field_1_8" class="gfield gfield--type-text gfield--width-full gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_8" ><label class='gfield_label gform-field-label' for='input_1_8'>Country<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_text'><input name='input_8' id='input_1_8' type='text' value='' class='large' aria-required="true" aria-invalid="false" /> </div></div><div id="field_1_3" class="gfield gfield--type-textarea gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_3" ><label class='gfield_label gform-field-label' for='input_1_3'>Tell us more about your needs and projects<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_textarea'><textarea name='input_3' id='input_1_3' class='textarea medium' maxlength='600' aria-required="true" aria-invalid="false" rows='10' cols='50'></textarea></div></div><fieldset id="field_1_6" class="gfield gfield--type-consent gfield--type-choice gfield--input-type-consent gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_6" ><legend class='gfield_label gform-field-label gfield_label_before_complex' >By submitting this form, PathoQuest will use the personal data you provide to handle your request. Read our Privacy Notice for more information.<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></legend><div class='ginput_container ginput_container_consent'><input name='input_6.1' id='input_1_6_1' type='checkbox' value='1' aria-required="true" aria-invalid="false" /> <label class="gform-field-label gform-field-label--type-inline gfield_consent_label" for='input_1_6_1' >I agree to the privacy policy.</label><input type='hidden' name='input_6.2' value='I agree to the privacy policy.' class='gform_hidden' /><input type='hidden' name='input_6.3' value='2' class='gform_hidden' /></div></fieldset><div id="field_1_9" class="gfield gfield--type-honeypot gform_validation_container field_sublabel_above gfield--has-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_9" ><label class='gfield_label gform-field-label' for='input_1_9'>Name</label><div class='gfield_description' id='gfield_description_1_9'>This field is for validation purposes and should be left unchanged.</div><div class='ginput_container'><input name='input_9' id='input_1_9' type='text' value='' autocomplete='new-password'/></div></div></div></div> <div class='gform_footer top_label'> <input type='submit' id='gform_submit_button_1' class='gform_button button et_pb_button' value='Submit' onclick='if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} ' onkeypress='if( event.keyCode == 13 ){ if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} jQuery("#gform_1").trigger("submit",[true]); }' /> <input type='hidden' class='gform_hidden' name='is_submit_1' value='1' /> <input type='hidden' class='gform_hidden' name='gform_submit' value='1' /> <input type='hidden' class='gform_hidden' name='gform_unique_id' value='' /> <input type='hidden' class='gform_hidden' name='state_1' value='WyJ7XCI2LjFcIjpcImI5ZDIyMDQ1YjdjYTViMTQ1NzYwODc1NmY3ZjRlMGYyXCIsXCI2LjJcIjpcIjJhYTFhOGQ0NDNiMTFjY2YzOGNlNTAyNmNlZmQ4ZWUxXCIsXCI2LjNcIjpcIjVlM2YzZThhYjhlOWZjZDU4YzVlNTc0ZmM4NTM0OWVmXCJ9IiwiYTc2ZjZhMzE2MTIzZTZjZThjZGQyOTM2ZWFmNGUwYzciXQ==' /> <input type='hidden' class='gform_hidden' name='gform_target_page_number_1' id='gform_target_page_number_1' value='0' /> <input type='hidden' class='gform_hidden' name='gform_source_page_number_1' id='gform_source_page_number_1' value='1' /> <input type='hidden' name='gform_field_values' value='' /> </div> <input type="hidden" id="ct_checkjs_daca41214b39c5dc66674d09081940f0" name="ct_checkjs" value="0" /><script >setTimeout(function(){var ct_input_name = "ct_checkjs_daca41214b39c5dc66674d09081940f0";if (document.getElementById(ct_input_name) !== null) {var ct_input_value = document.getElementById(ct_input_name).value;document.getElementById(ct_input_name).value = document.getElementById(ct_input_name).value.replace(ct_input_value, '2085491878');}}, 1000);</script><label class="apbct_special_field" id="apbct_label_id94140" for="apbct__email_id__gravity_form_94140">94140</label><input id="apbct__email_id__gravity_form_94140" class="apbct_special_field apbct__email_id__gravity_form" autocomplete="off" name="apbct__email_id__gravity_form_94140" type="text" value="94140" size="30" apbct_event_id="94140" maxlength="200" /><input id="apbct_event_id" class="apbct_special_field" name="apbct_event_id" type="hidden" value="94140" /></form> </div><script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() {gformInitSpinner( 1, 'https://pathoquest.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery('#gform_ajax_frame_1').on('load',function(){var contents = jQuery(this).contents().find('*').html();var is_postback = contents.indexOf('GF_AJAX_POSTBACK') >= 0;if(!is_postback){return;}var form_content = jQuery(this).contents().find('#gform_wrapper_1');var is_confirmation = jQuery(this).contents().find('#gform_confirmation_wrapper_1').length > 0;var is_redirect = contents.indexOf('gformRedirect(){') >= 0;var is_form = form_content.length > 0 && ! is_redirect && ! is_confirmation;var mt = parseInt(jQuery('html').css('margin-top'), 10) + parseInt(jQuery('body').css('margin-top'), 10) + 100;if(is_form){jQuery('#gform_wrapper_1').html(form_content.html());if(form_content.hasClass('gform_validation_error')){jQuery('#gform_wrapper_1').addClass('gform_validation_error');} else {jQuery('#gform_wrapper_1').removeClass('gform_validation_error');}setTimeout( function() { /* delay the scroll by 50 milliseconds to fix a bug in chrome */ }, 50 );if(window['gformInitDatepicker']) {gformInitDatepicker();}if(window['gformInitPriceFields']) {gformInitPriceFields();}var current_page = jQuery('#gform_source_page_number_1').val();gformInitSpinner( 1, 'https://pathoquest.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery(document).trigger('gform_page_loaded', [1, current_page]);window['gf_submitting_1'] = false;}else if(!is_redirect){var confirmation_content = jQuery(this).contents().find('.GF_AJAX_POSTBACK').html();if(!confirmation_content){confirmation_content = contents;}jQuery('#gform_wrapper_1').replaceWith(confirmation_content);jQuery(document).trigger('gform_confirmation_loaded', [1]);window['gf_submitting_1'] = false;wp.a11y.speak(jQuery('#gform_confirmation_message_1').text());}else{jQuery('#gform_1').append(contents);if(window['gformRedirect']) {gformRedirect();}}jQuery(document).trigger("gform_pre_post_render", [{ formId: "1", currentPage: "current_page", abort: function() { this.preventDefault(); } }]); if (event && event.defaultPrevented) { return; } const gformWrapperDiv = document.getElementById( "gform_wrapper_1" ); if ( gformWrapperDiv ) { const visibilitySpan = document.createElement( "span" ); visibilitySpan.id = "gform_visibility_test_1"; gformWrapperDiv.insertAdjacentElement( "afterend", visibilitySpan ); } const visibilityTestDiv = document.getElementById( "gform_visibility_test_1" ); let postRenderFired = false; function triggerPostRender() { if ( postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [1, current_page] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 1, currentPage: current_page } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); }; var callNow = immediate && !timeout; clearTimeout( timeout ); timeout = setTimeout( later, wait ); if ( callNow ) func.apply( context, args ); }; } const debouncedTriggerPostRender = debounce( function() { triggerPostRender(); }, 200 ); if ( visibilityTestDiv && visibilityTestDiv.offsetParent === null ) { const observer = new MutationObserver( ( mutations ) => { mutations.forEach( ( mutation ) => { if ( mutation.type === 'attributes' && visibilityTestDiv.offsetParent !== null ) { debouncedTriggerPostRender(); observer.disconnect(); } }); }); observer.observe( document.body, { attributes: true, childList: false, subtree: true, attributeFilter: [ 'style', 'class' ], }); } else { triggerPostRender(); } } );} ); /* ]]> */ </script> </section> </div> </div><div class="et_pb_module et_pb_text et_pb_text_36 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><strong>Sign up for our latest news</strong></p></div> </div> <div id="et_pb_contact_form_1" class="et_pb_module et_pb_contact_form_1 et_pb_contact_form_container clearfix et_pb_text_align_left" data-form_unique_num="1" 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0L176 189.28 75.93 89.21c-12.28-12.28-32.19-12.28-44.48 0L9.21 111.45c-12.28 12.28-12.28 32.19 0 44.48L109.28 256 9.21 356.07c-12.28 12.28-12.28 32.19 0 44.48l22.24 22.24c12.28 12.28 32.2 12.28 44.48 0L176 322.72l100.07 100.07c12.28 12.28 32.2 12.28 44.48 0l22.24-22.24c12.28-12.28 12.28-32.19 0-44.48L242.72 256z"></path></svg> </div> </div> <div class="cmplz-divider cmplz-divider-header"></div> <div class="cmplz-body"> <div class="cmplz-message" id="cmplz-message-1-optin">To provide the best experiences, we use technologies like cookies to store and/or access device information. 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32.75 0 45.25l-192 192C240.4 412.9 232.2 416 224 416z"/></svg> </span> </span> </summary> <div class="cmplz-description"> <span class="cmplz-description-marketing">The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.</span> </div> </details> </div> </div> <div class="cmplz-links cmplz-information"> <a class="cmplz-link cmplz-manage-options cookie-statement" href="#" data-relative_url="#cmplz-manage-consent-container">Manage options</a> <a class="cmplz-link cmplz-manage-third-parties cookie-statement" href="#" data-relative_url="#cmplz-cookies-overview">Manage services</a> <a class="cmplz-link cmplz-manage-vendors tcf cookie-statement" href="#" data-relative_url="#cmplz-tcf-wrapper">Manage {vendor_count} vendors</a> <a class="cmplz-link cmplz-external cmplz-read-more-purposes tcf" target="_blank" rel="noopener noreferrer nofollow" href="https://cookiedatabase.org/tcf/purposes/">Read more about these purposes</a> </div> <div class="cmplz-divider cmplz-footer"></div> <div class="cmplz-buttons"> <button class="cmplz-btn cmplz-accept">Accept</button> <button class="cmplz-btn cmplz-deny">Deny</button> <button class="cmplz-btn cmplz-view-preferences">View preferences</button> <button class="cmplz-btn cmplz-save-preferences">Save preferences</button> <a class="cmplz-btn cmplz-manage-options tcf cookie-statement" href="#" data-relative_url="#cmplz-manage-consent-container">View preferences</a> </div> <div class="cmplz-links cmplz-documents"> <a class="cmplz-link cookie-statement" href="#" data-relative_url="">{title}</a> <a class="cmplz-link privacy-statement" href="#" data-relative_url="">{title}</a> <a class="cmplz-link impressum" href="#" data-relative_url="">{title}</a> </div> </div> </div> <div id="cmplz-manage-consent" data-nosnippet="true"><button class="cmplz-btn cmplz-hidden cmplz-manage-consent 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top","position_from_trigger":false,"position_top":"100","position_left":"0","position_bottom":"0","position_right":"0","position_fixed":false,"animation_type":"fade","animation_speed":"350","animation_origin":"center top","overlay_zindex":false,"zindex":"1999999999"},"close":{"text":"","button_delay":"0","overlay_click":false,"esc_press":false,"f4_press":false},"click_open":[]}}"> <div id="popmake-987509807" class="pum-container popmake theme-987509798 pum-responsive pum-responsive-medium responsive size-medium"> <div class="pum-content popmake-content" tabindex="0"> <script type="text/javascript"></script> <div class='gf_browser_chrome gform_wrapper gravity-theme gform-theme--no-framework' data-form-theme='gravity-theme' data-form-index='0' id='gform_wrapper_10' > <div class='gform_heading'> <h2 class="gform_title">Analytical Methods Validation Head contact form</h2> <p class='gform_description'></p> </div><form method='post' enctype='multipart/form-data' id='gform_10' action='/services/integration-site-analysis/' data-formid='10' novalidate> <input type='hidden' class='gforms-pum' value='{"closepopup":false,"closedelay":0,"openpopup":false,"openpopup_id":0}' /> <div class='gform-body gform_body'><div id='gform_fields_10' class='gform_fields top_label form_sublabel_above description_above validation_below'><fieldset id="field_10_1" class="gfield gfield--type-name gfield--input-type-name gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_10_1" ><legend class='gfield_label gform-field-label gfield_label_before_complex' >Name<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></legend><div class='ginput_complex ginput_container ginput_container--name no_prefix has_first_name no_middle_name has_last_name no_suffix gf_name_has_2 ginput_container_name gform-grid-row' id='input_10_1'> <span 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