Calnexin Antibody (3H4A7) (NBP1-47546): Novus Biologicals

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5.1724696046-473375462.1712005244" style="margin: 0 10px;" target="_blank"><img alt="Novus Biologicals products are now on bio-techne.com" src="https://images.novusbio.com/design/ready-to-buy-novus-products-login.png" style="height:120px; width:940px" /></a></div> </div> </div> </div> <h1>Calnexin Antibody (3H4A7)</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left"><a href="#PublicationSection"><div class="sprite_pubBook_large"></div></a> <a href="#PublicationSection" class="bottom2"><span class="ctr">(3 Publications)</span></a></div><div class="ds_info float_left"> <a href="/common-name/calnexin"><div class="sprite_allRelated"></div></a> <a href="/common-name/calnexin" class="bottom4"><span class="ctr">See All Related</span></a> </div><div class="ds_info float_left"><a href="/reviews/NBP1-47546"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/NBP1-47546" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="2" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Calnexin-Antibody-3H4A7-Western-Blot-NBP1-47546-img0002.jpg" class="top_images" rel="gallery" title="Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate." > <img src="//images.novusbio.com/images/Calnexin-Antibody-3H4A7-Western-Blot-NBP1-47546-img0002.jpg" class="big_thumb" alt="Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate." title="Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Calnexin-Antibody-3H4A7-Immunocytochemistry-Immunofluorescence-NBP1-47546-img0003.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546] - Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green)." > <img src="//images.novusbio.com/images/Calnexin-Antibody-3H4A7-Immunocytochemistry-Immunofluorescence-NBP1-47546-img0003.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546] - Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green)." title="Immunocytochemistry/Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546] - Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green)." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546] - Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green).</div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='ELISA'>ELISA</span>, <span title='Immunocytochemistry/ Immunofluorescence'>ICC/IF</span></td> </tr><tr class="lined"> <td><strong>Clone</strong></td> <td><div>3H4A7</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Monoclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Mouse</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr></table></div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="" data-catnum="NBP1-47546" data-catnums="NBP1-47546,NBP1-47546SS" data-disabled='["AE","AL","BA","CH","FI","IL","IQ","JO","KW","KZ","LB","MK","OM","QA","SA","TR","UA","UZ","YU"]'><noscript><form autocomplete="off" action="/products/calnexin-antibody-3h4a7_nbp1-47546" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # NBP1-47546SS">0.025 ml</div><div data-msg='' class="atc_catnum">NBP1-47546SS</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br 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data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>Calnexin Antibody (3H4A7) Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div>A synthetic peptide (CEAAEERPWLWVVYILTVAL) corresponding to a region of human Calnexin, conjugated to KLH. [UniProt# P27824]</div></td> </tr><tr class="lined"> <td><strong>Marker</strong></td> <td><div>Endoplasmic Reticulum Membrane Marker</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG2b</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Monoclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Mouse</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>CANX</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Unpurified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>ELISA 1:10000</li><li>Immunocytochemistry/ Immunofluorescence 1:200-1:1000</li><li>Western Blot 1:500-1:2000</li> </ul> </td> </tr><tr class="lined"> <td><strong>Application Notes</strong></td> <td><div>This Calnexin (3H4A7) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and ELISA.</div></td> </tr><tr class="lined"> <td><strong>Theoretical MW</strong></td> <td><div>90 kDa.<br> Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.</div></td> </tr> <tr class="lined"> <td><strong>Control</strong></td> <td> <table class="wide ds_altTable"><tr> <td style="width:80%"><a href="/hela-lysate_nb800-pc1">HeLa Whole Cell Lysate</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NB800-PC1" data-return="calnexin-antibody-3h4a7_nbp1-47546" data-produrl="calnexin-antibody-3h4a7_nbp1-47546" data-catnums="NB800-PC1" ></div> </td> </tr><tr> <td style="width:80%"><a href="/mcf-7-lysate_nb820-59465">MCF-7 Whole Cell Lysate</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NB820-59465" data-return="calnexin-antibody-3h4a7_nbp1-47546" data-produrl="calnexin-antibody-3h4a7_nbp1-47546" data-catnums="NB820-59465" ></div> </td> </tr></table> </td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> NBP1-47546 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="WB">WB</a> </div><div class="information-data">1 publication</div></li></ul></td> </tr> </table> </td> </tr> </table> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div>Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.</div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>Ascites</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>0.03% Sodium Azide</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Unpurified</div></td> </tr></table></div><h2 class="greyHead2">Alternate Names for Calnexin Antibody (3H4A7)</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>calnexin</li><li>CNX</li><li>IP90FLJ26570</li><li>Major histocompatibility complex class I antigen-binding protein p88</li><li>P90</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div>Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.</div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are <a href="/support/quality-assurance.html">guaranteed</a> for 1 year from date of receipt.</div> </div><h2 class="greyHead2">Customers Who Viewed This Item Also Viewed...</h2> <a id="custalsoviewed"></a><div id="cav_prev"><div class="sprite_leftArrow"></div></div> <div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout=0 data-cycle-prev="#cav_prev" data-cycle-next="#cav_next" data-cycle-slides="div.ds_cav_group" id="ds_customer_also_viewed"><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB600-101"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/calreticulin-antibody_nb600-101" style="overflow: hidden; width: 280px;" title="Calreticulin Antibody - BSA Free">Calreticulin<br> Antibody - BSA Free</a>    </div><div class="sub-header">NB600-101</div> <div class="images_customers"><div id="ss_prev_cav0" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev_cav0" data-cycle-next="#ss_next_cav0" data-cycle-slides="div.big_thumb_cust_container" data-index=0 ><div class="big_thumb_cust_container"> <a href="/products/calreticulin-antibody_nb600-101" title="Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Calreticulin-Antibody-Knockdown-Validated-NB600-101-img0011.jpg" alt="Knockdown Validated Calreticulin Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/calreticulin-antibody_nb600-101" title="Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Calreticulin-Antibody-Western-Blot-NB600-101-img0006.jpg" alt="Western Blot Calreticulin Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/calreticulin-antibody_nb600-101" title="Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-101-img0007.jpg" alt="Immunocytochemistry/ Immunofluorescence Calreticulin Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav0" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-0" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/calreticulin-antibody_nb600-101#PublicationSection"> <div class="sprite_pubBook"></div> (28 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Ha, Hu, Mu, Pm, Rt</div><div class="tiny"><strong>Applications:</strong> B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB79671"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/rsk1-antibody-1024a_mab79671" style="overflow: hidden; width: 280px;" title="RSK1 [p Ser380] Antibody (1024A) [Unconjugated]">RSK1 [p Ser380]<br> Antibody (1024A) [Unc ...</a>    </div><div class="sub-header">MAB79671</div> <div class="images_customers"><div id="ss_prev_cav1" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-1" data-cycle-prev="#ss_prev_cav1" data-cycle-next="#ss_next_cav1" data-cycle-slides="div.big_thumb_cust_container" data-index=1 ><div class="big_thumb_cust_container"> <a href="/products/rsk1-antibody-1024a_mab79671" title="RSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line, unstimulated (panels B and D) or stimulated with PMA (panels A and C), using Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, upper panels; Catalog # NL004) and counterstained with DAPI (blue, lower panels). Specific staining was localized to plasma membrances and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/RSK1_MAB79671_Immunocytochemistry_15894.jpg" alt="Immunocytochemistry RSK1 [p Ser380] Antibody (1024A) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rsk1-antibody-1024a_mab79671" title="Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, and loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-RSK1 (S380) at approximately 90&amp;nbsp;kDa (as indicated) using 1 μg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671). This experiment was conducted under reducing conditions and using the 12-230&amp;nbsp;kDa separation system." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/16242.jpg" alt="Simple Western RSK1 [p Ser380] Antibody (1024A) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rsk1-antibody-1024a_mab79671" title="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S380) at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/RSK1_MAB79671_Western_Blot_14193.jpg" alt="Western Blot RSK1 [p Ser380] Antibody (1024A) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav1" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-1" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/rsk1-antibody-1024a_mab79671#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-06274"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/grp78-hspa5-antibody_nbp1-06274" style="overflow: hidden; width: 280px;" title="GRP78/HSPA5 Antibody - BSA Free">GRP78/HSPA5<br> Antibody - BSA Free</a>    </div><div class="sub-header">NBP1-06274</div> <div class="images_customers"><div id="ss_prev_cav2" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-2" data-cycle-prev="#ss_prev_cav2" data-cycle-next="#ss_next_cav2" data-cycle-slides="div.big_thumb_cust_container" data-index=2 ><div class="big_thumb_cust_container"> <a href="/products/grp78-hspa5-antibody_nbp1-06274" title="Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on Jurkat cells with Alexa Fluor 647 conjugate of GPR78/HSPA5 antibody NBP1-06274AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0008.jpg" alt="Flow Cytometry GRP78/HSPA5 Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/grp78-hspa5-antibody_nbp1-06274" title="Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0012.jpg" alt="Flow Cytometry GRP78/HSPA5 Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/grp78-hspa5-antibody_nbp1-06274" title="Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06274-img0013.jpg" alt="Immunocytochemistry/ Immunofluorescence GRP78/HSPA5 Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/grp78-hspa5-antibody_nbp1-06274#ReviewsSection"> <div class="sprite_4Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/grp78-hspa5-antibody_nbp1-06274#PublicationSection"> <div class="sprite_pubBook"></div> (43 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze</div><div class="tiny"><strong>Applications:</strong> Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB100-1595"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/ribosomal-protein-s6-rps6-antibody_nb100-1595" style="overflow: hidden; width: 280px;" title="Ribosomal Protein S6/RPS6 Antibody">Ribosomal Protein<br> S6/RPS6 Antibody</a>    </div><div class="sub-header">NB100-1595</div> <div class="images_customers"><div id="ss_prev_cav3" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/ribosomal-protein-s6-rps6-antibody_nb100-1595" title="Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB" class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/Ribosomal-Protein-S6-RPS6-Antibody-Immunohistochemistry-NB100-1595-img0009.jpg" alt="Immunohistochemistry-Paraffin Ribosomal Protein S6/RPS6 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ribosomal-protein-s6-rps6-antibody_nb100-1595" title="Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous" class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/Ribosomal-Protein-S6-RPS6-Antibody-Western-Blot-NB100-1595-img0007.jpg" alt="Western Blot Ribosomal Protein S6/RPS6 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ribosomal-protein-s6-rps6-antibody_nb100-1595" title="Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody NB100-1595 (lot NB100-1595-2) used for IP at 6 ug per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1595-1). For blotting immunoprecipitated RPS6, NB100-1595 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/Ribosomal-Protein-S6-RPS6-Antibody-Immunoprecipitation-NB100-1595-img0008.jpg" alt="Immunoprecipitation Ribosomal Protein S6/RPS6 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav3" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-3" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/ribosomal-protein-s6-rps6-antibody_nb100-1595#ReviewsSection"> <div class="sprite_4Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/ribosomal-protein-s6-rps6-antibody_nb100-1595#PublicationSection"> <div class="sprite_pubBook"></div> (6 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, IP, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-20237"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/rsk2-antibody_nbp2-20237" style="overflow: hidden; width: 280px;" title="RSK2 Antibody">RSK2 Antibody</a>    </div><div class="sub-header">NBP2-20237</div> <div class="images_customers"><div id="ss_prev_cav4" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/rsk2-antibody_nbp2-20237" title="Western Blot: RSK2 Antibody [NBP2-20237] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:2000." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/RSK2-Antibody-Western-Blot-NBP2-20237-img0001.jpg" alt="Western Blot RSK2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rsk2-antibody_nbp2-20237" title="Western Blot: RSK2 Antibody [NBP2-20237] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/RSK2-Antibody-Western-Blot-NBP2-20237-img0002.jpg" alt="Western Blot RSK2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rsk2-antibody_nbp2-20237" title="Western Blot: RSK2 Antibody [NBP2-20237] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE gel, diluted at 1:10000." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/RSK2-Antibody-Western-Blot-NBP2-20237-img0003.jpg" alt="Western Blot RSK2 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1230"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk2-antibody_af1230" style="overflow: hidden; width: 280px;" title="ERK2 Antibody [Unconjugated]">ERK2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF1230</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg" alt="Western Blot ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title=" ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg" alt="Immunohistochemistry ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg" alt="Knockout Validated ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk2-antibody_af1230#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, KO, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-84796"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erp57-pdia3-antibody_nbp1-84796" style="overflow: hidden; width: 280px;" title="ERp57/PDIA3 Antibody">ERp57/PDIA3<br> Antibody</a>    </div><div class="sub-header">NBP1-84796</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/erp57-pdia3-antibody_nbp1-84796" title="Western Blot: ERp57/PDIA3 Antibody [NBP1-84796] - Analysis using Anti-PDIA3 antibody NBP1-84796 (A) shows similar pattern to independent antibody NBP1-84797 (B)." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERp57-PDIA3-Antibody-Western-Blot-NBP1-84796-img0014.jpg" alt="Western Blot ERp57/PDIA3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erp57-pdia3-antibody_nbp1-84796" title="Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84796] - Staining of human gastrointestinal, prostate, skeletal muscle and thyroid gland using Anti-PDIA3 antibody NBP1-84796 (A) shows similar protein distribution across tissues to independent antibody NBP1-84797 (B)." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84796-img0018.jpg" alt="Immunohistochemistry-Paraffin ERp57/PDIA3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erp57-pdia3-antibody_nbp1-84796" title="Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84796] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84796-img0019.jpg" alt="Immunohistochemistry-Paraffin ERp57/PDIA3 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erp57-pdia3-antibody_nbp1-84796#PublicationSection"> <div class="sprite_pubBook"></div> (4 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, KD, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-52555"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/rsk3-antibody-3c4c8_nbp2-52555" style="overflow: hidden; width: 280px;" title="RSK3 Antibody (3C4C8) - BSA Free">RSK3 Antibody<br> (3C4C8) - BSA Free</a>    </div><div class="sub-header">NBP2-52555</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/rsk3-antibody-3c4c8_nbp2-52555" title="Western Blot: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis using RPS6KA2 mAb against HEK293 (1) and RPS6KA2 (AA: 415-734)-hIgGFc transfected HEK293 (2) cell lysate." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/RSK3-Antibody-3C4C8-Western-Blot-NBP2-52555-img0007.jpg" alt="Western Blot RSK3 Antibody (3C4C8) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rsk3-antibody-3c4c8_nbp2-52555" title="ELISA: RSK3 Antibody (3C4C8) [NBP2-52555] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/RSK3-Antibody-3C4C8-ELISA-NBP2-52555-img0008.jpg" alt="ELISA RSK3 Antibody (3C4C8) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rsk3-antibody-3c4c8_nbp2-52555" title="Western Blot: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis using RSK3 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/RSK3-Antibody-3C4C8-Western-Blot-NBP2-52555-img0009.jpg" alt="Western Blot RSK3 Antibody (3C4C8) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/rsk3-antibody-3c4c8_nbp2-52555#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-22203"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk1-antibody-1e5_nbp2-22203" style="overflow: hidden; width: 280px;" title="ERK1 Antibody (1E5) - BSA Free">ERK1 Antibody<br> (1E5) - BSA Free</a>    </div><div class="sub-header">NBP2-22203</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red)." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg" alt="Flow Cytometry ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg" alt="Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk1-antibody-1e5_nbp2-22203#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Pm, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-87799"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tbp7-antibody_nbp1-87799" style="overflow: hidden; width: 280px;" title="Tbp7 Antibody">Tbp7 Antibody</a>    </div><div class="sub-header">NBP1-87799</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/tbp7-antibody_nbp1-87799" title="Immunohistochemistry-Paraffin: Tbp7 Antibody [NBP1-87799] - Staining of human gall bladder shows nuclear positivity in glandular cells." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Tbp7-Antibody-Immunohistochemistry-Paraffin-NBP1-87799-img0001.jpg" alt="Immunohistochemistry-Paraffin Tbp7 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tbp7-antibody_nbp1-87799" title="Western Blot: Tbp7 Antibody [NBP1-87799] - Analysis in human cell line A-549." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Tbp7-Antibody-Western-Blot-NBP1-87799-img0007.jpg" alt="Western Blot Tbp7 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tbp7-antibody_nbp1-87799" title="Western Blot: Tbp7 Antibody [NBP1-87799] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Tbp7-Antibody-Western-Blot-NBP1-87799-img0010.jpg" alt="Western Blot Tbp7 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/tbp7-antibody_nbp1-87799#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1543"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/bmp-3b-gdf-10-antibody_af1543" style="overflow: hidden; width: 280px;" title="BMP-3b/GDF-10 Antibody [Unconjugated]">BMP-3b/GDF-10<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF1543</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/bmp-3b-gdf-10-antibody_af1543" title="BMP&amp;#x2011;3b/GDF&amp;#x2011;10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Goat Anti-Human BMP&amp;#x2011;3b/GDF&amp;#x2011;10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1543) at 10&amp;nbsp;µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte&amp;trade; HRP Polymer Antibody (Catalog&amp;#160;# VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog&amp;#160;# VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/antibody/af1543_human-bmp-3b-gdf-10-affinity-purified-polyclonal-ab-immunocytochemistry-133202414504.jpg" alt="Immunohistochemistry BMP-3b/GDF-10 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/bmp-3b-gdf-10-antibody_af1543#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-01346"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/sec14l2-antibody-oti4h2_nbp2-01346" style="overflow: hidden; width: 280px;" title="SEC14L2 Antibody (OTI4H2)">SEC14L2 Antibody<br> (OTI4H2)</a>    </div><div class="sub-header">NBP2-01346</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/sec14l2-antibody-oti4h2_nbp2-01346" title="Flow Cytometry: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SEC14L2 antibody, and then analyzed by flow cytometry." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/SEC14L2-Antibody-4H2-Flow-Cytometry-NBP2-01346-img0001.jpg" alt="Flow Cytometry SEC14L2 Antibody (OTI4H2)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sec14l2-antibody-oti4h2_nbp2-01346" title="Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/SEC14L2-Antibody-4H2-Western-Blot-NBP2-01346-img0002.jpg" alt="Western Blot SEC14L2 Antibody (OTI4H2)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sec14l2-antibody-oti4h2_nbp2-01346" title="Immunohistochemistry-Paraffin: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/SEC14L2-Antibody-4H2-Immunohistochemistry-Paraffin-NBP2-01346-img0003.jpg" alt="Immunohistochemistry-Paraffin SEC14L2 Antibody (OTI4H2)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Ca, Hu, Pm, Mu, Pm, Rt</div><div class="tiny"><strong>Applications:</strong> Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF467"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/ephb2-antibody_af467" style="overflow: hidden; width: 280px;" title="EphB2 Antibody [Unconjugated]">EphB2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF467</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title=" Simple Western lane view shows lysates of COLO&amp;nbsp;205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for EphB2 at approximately 139 and 146&amp;nbsp;kDa (as indicated) using 20&amp;nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg" alt="Simple Western EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title="Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg" alt="Western Blot EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title="EphB2 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg" alt="Immunocytochemistry EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/ephb2-antibody_af467#ReviewsSection"> <div class="sprite_4Stars"></div> (5 Reviews</a>) </div> <div class="tiny"> <a href="/products/ephb2-antibody_af467#PublicationSection"> <div class="sprite_pubBook"></div> (62 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow, ICC, IHC, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB4614"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/calcitonin-r-antibody-462802_mab4614" style="overflow: hidden; width: 280px;" title="Calcitonin R Antibody (462802) [Unconjugated]">Calcitonin R<br> Antibody (462802) [Un ...</a>    </div><div class="sub-header">MAB4614</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/calcitonin-r-antibody-462802_mab4614" title="Calcitonin R knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Calcitonin R knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/Calcitonin_R_MAB4614_Knockout_Validated_23018.jpg" alt="Knockout Validated Calcitonin R Antibody (462802) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/calcitonin-r-antibody-462802_mab4614" title="MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin&amp;nbsp;R Monoclonal Antibody (Catalog&amp;nbsp;# MAB4614, filled histogram) or isotype control antibody (Catalog&amp;nbsp;# MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog&amp;nbsp;# F0102B)." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/Calcitonin_R_MAB4614_Flow_Cytometry_8474.jpg" alt="Flow Cytometry Calcitonin R Antibody (462802) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/calcitonin-r-antibody-462802_mab4614" title="Effects of AA and DHA on actin ring formation and CTR expression.Osteoclast differentiation was stimulated in CD14+ monocytes by the addition of 25 ng ml-1 M-CSF and 30 ng ml-1 RANKL as described in Materials and Methods. Vehicle (0.08% ethanol) or LCPUFAs (40 μM) were added from day 3 in differentiating osteoclasts and from the onset of resorption (day 12–14) in mature osteoclasts. After 3 weeks of culture, cells were fixed and stained with Hoechst (blue) for nuclei, phalloidin (red) for actin rings, and anti-CTR antibody (green). The results are representative of three independent experiments conducted in triplicate. Scale bar = 200 μm. Diff Ocl—differentiating osteoclasts. Mat Ocl—mature osteoclasts. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25867515), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/mab4614_human-calcitonin-r-mab-clone-462802-41202410484315.jpg" alt="Immunocytochemistry/ Immunofluorescence Calcitonin R Antibody (462802) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/calcitonin-r-antibody-462802_mab4614#PublicationSection"> <div class="sprite_pubBook"></div> (6 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow, KO</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-11567"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/slc6a8-antibody_nbp2-11567" style="overflow: hidden; width: 280px;" title="SLC6A8 Antibody">SLC6A8 Antibody</a>    </div><div class="sub-header">NBP2-11567</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/slc6a8-antibody_nbp2-11567" title="Immunohistochemistry-Paraffin: SLC6A8 Antibody [NBP2-11567] - Human kidney. Antibody at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH6, AP-staining." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/SLC6A8-Antibody-Immunohistochemistry-Paraffin-NBP2-11567-img0003.jpg" alt="Immunohistochemistry-Paraffin SLC6A8 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/slc6a8-antibody_nbp2-11567" title="Western Blot: SLC6A8 Antibody [NBP2-11567] - Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/ml, 1 hr incubation. Detected by chemiluminescence." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/SLC6A8-Antibody-Western-Blot-NBP2-11567-img0002.jpg" alt="Western Blot SLC6A8 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/slc6a8-antibody_nbp2-11567#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, PEP-ELISA, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00006890-M04"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tap1-antibody-3d4_h00006890-m04" style="overflow: hidden; width: 280px;" title="TAP1 Antibody (3D4)">TAP1 Antibody (3D4)</a>    </div><div class="sub-header">H00006890-M04</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/tap1-antibody-3d4_h00006890-m04" title="Flow Cytometry: TAP1 Antibody (3D4) [H00006890-M04] - Intracellular flow cytometric analysis of TAP1 antibody H00006890-M04 (orange filled) on interferon gamma treated HCT116 cells. Red dotted line in histogram represents unstained and blue filled line represents isotype control. Flow cytometry image added from a verified customer review." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/TAP1-Antibody-3D4-Flow-Cytometry-H00006890-M04-img0002.jpg" alt="Flow Cytometry TAP1 Antibody (3D4)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tap1-antibody-3d4_h00006890-m04" title="Immunocytochemistry/Immunofluorescence: TAP1 Antibody (3D4) [H00006890-M04] - Analysis of monoclonal antibody to TAP1 on HeLa cell. Antibody concentration 10 ug/ml" class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/TAP1-Antibody-3D4-Immunocytochemistry-Immunofluorescence-H00006890-M04-img0001.jpg" alt="Immunocytochemistry/ Immunofluorescence TAP1 Antibody (3D4)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/tap1-antibody-3d4_h00006890-m04#ReviewsSection"> <div class="sprite_4Stars"></div> (1 Review</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA, Flow, ICC/IF, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-89394"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/txndc15-antibody_nbp1-89394" style="overflow: hidden; width: 280px;" title="TXNDC15 Antibody">TXNDC15 Antibody</a>    </div><div class="sub-header">NBP1-89394</div> <div class="images_customers"><div id="ss_prev_cav16" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/txndc15-antibody_nbp1-89394" title="Immunocytochemistry/Immunofluorescence: TXNDC15 Antibody [NBP1-89394] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/TXNDC15-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89394-img0004.jpg" alt="Immunocytochemistry/ Immunofluorescence TXNDC15 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/txndc15-antibody_nbp1-89394" title="Immunohistochemistry-Paraffin: TXNDC15 Antibody [NBP1-89394] - Staining of human colon shows cytoplasmic positivity with a granular pattern in glandular cells." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/TXNDC15-Antibody-Immunohistochemistry-Paraffin-NBP1-89394-img0002.jpg" alt="Immunohistochemistry-Paraffin TXNDC15 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav16" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-16" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-86968"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tapasin-antibody_nbp1-86968" style="overflow: hidden; width: 280px;" title="Tapasin Antibody">Tapasin Antibody</a>    </div><div class="sub-header">NBP1-86968</div> <div class="images_customers"><div id="ss_prev_cav17" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/tapasin-antibody_nbp1-86968" title="Immunohistochemistry-Paraffin: Tapasin Antibody [NBP1-86968] - Staining of human skeletal muscle shows very weak positivity in endoplasmic reticulum in myocytes." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/Tapasin-Antibody-Immunohistochemistry-Paraffin-NBP1-86968-img0001.jpg" alt="Immunohistochemistry-Paraffin Tapasin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tapasin-antibody_nbp1-86968" title="Immunohistochemistry-Paraffin: Tapasin Antibody [NBP1-86968] - Staining of human duodenum shows strong positivity in endoplasmic reticulum in glandular cells." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/Tapasin-Antibody-Immunohistochemistry-Paraffin-NBP1-86968-img0002.jpg" alt="Immunohistochemistry-Paraffin Tapasin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tapasin-antibody_nbp1-86968" title="Immunohistochemistry-Paraffin: Tapasin Antibody [NBP1-86968] - Staining of human fallopian tube shows strong positivity in endoplasmic reticulum in neutrophils." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/Tapasin-Antibody-Immunohistochemistry-Paraffin-NBP1-86968-img0003.jpg" alt="Immunohistochemistry-Paraffin Tapasin Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav17" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-17" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/tapasin-antibody_nbp1-86968#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB300-619"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/gp96-hsp90b1-grp94-antibody-9g10_nb300-619" style="overflow: hidden; width: 280px;" title="gp96/HSP90B1/GRP94 Antibody (9G10)">gp96/HSP90B1/GRP94<br> Antibody (9G10)</a>    </div><div class="sub-header">NB300-619</div> <div class="images_customers"><div id="ss_prev_cav18" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-18" data-cycle-prev="#ss_prev_cav18" data-cycle-next="#ss_next_cav18" data-cycle-slides="div.big_thumb_cust_container" data-index=18 ><div class="big_thumb_cust_container"> <a href="/products/gp96-hsp90b1-grp94-antibody-9g10_nb300-619" title="Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - GR94 in human primary fibroblasts, ER staining. Primary antibody incubation: 1:250 in 10% Serum in PBST (0.1% Triton) O/N 4C. Secondary: Donkey anti-rat 647 1:500 2h RT. ICC/IF image submitted by a verifed customer review." class="cust_viewed_18" > <img loading="lazy" src="https://images.novusbio.com/images/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0032.jpg" alt="Immunocytochemistry/ Immunofluorescence gp96/HSP90B1/GRP94 Antibody (9G10)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gp96-hsp90b1-grp94-antibody-9g10_nb300-619" title="Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated." class="cust_viewed_18" > <img loading="lazy" src="https://images.novusbio.com/images/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0017.jpg" alt="Immunocytochemistry/ Immunofluorescence gp96/HSP90B1/GRP94 Antibody (9G10)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gp96-hsp90b1-grp94-antibody-9g10_nb300-619" title="Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in Hela Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated." class="cust_viewed_18" > <img loading="lazy" src="https://images.novusbio.com/images/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0018.jpg" alt="Immunocytochemistry/ Immunofluorescence gp96/HSP90B1/GRP94 Antibody (9G10)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav18" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-18" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/gp96-hsp90b1-grp94-antibody-9g10_nb300-619#ReviewsSection"> <div class="sprite_5Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/gp96-hsp90b1-grp94-antibody-9g10_nb300-619#PublicationSection"> <div class="sprite_pubBook"></div> (5 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Ch, Ha, Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC, ICC/IF, IHC, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB25031"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/cftr-antibody-24-1_mab25031" style="overflow: hidden; width: 280px;" title="CFTR Antibody (24-1) [Unconjugated] - C-terminus">CFTR Antibody<br> (24-1) [Unconjugated] ...</a>    </div><div class="sub-header">MAB25031</div> <div class="images_customers"><div id="ss_prev_cav19" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-19" data-cycle-prev="#ss_prev_cav19" data-cycle-next="#ss_next_cav19" data-cycle-slides="div.big_thumb_cust_container" data-index=19 ><div class="big_thumb_cust_container"> <a href="/products/cftr-antibody-24-1_mab25031" title="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell &amp; Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_19" > <img loading="lazy" src="https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg" alt="Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cftr-antibody-24-1_mab25031" title="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_19" > <img loading="lazy" src="https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg" alt="Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cftr-antibody-24-1_mab25031" title="COMMD1 and CFTR interact in mammalian cells.(A) Sequences of ICL3 in other species from fish to primates. Asterisks indicate the position of two class II mutations: S945L and D979A. Identity of amino acids between the different proteins are boxed in black, conserved residues are boxed in dark gray and semi-conserved substitutions in light gray. (B) Representative gels for the same co-immunoprecipitation experiments in HT-29 cells expressing endogenous CFTR and COMMD1. Lysates from HT-29 cells were immunoprecipitated (IP) with either 0.8 µg of anti-COMMD1 mAb (Abnova), 0.8 µg of anti-CFTR mAb (MAB25031, R&amp;D Systems) or with 0.8 µg anti-mouse IgG as a control. Each immunoprecipitation sample was then split in half and loaded onto an 8% SDS-PAGE for CFTR detection and 11% SDS-PAGE for COMMD1 detection. Both gels were transferred to PVDF membrane and subjected to immunoblotting (IB). The 8% SDS-PAGE membrane was probed with anti-CFTR mAb (MM13-4) and the 11% SDS-PAGE membrane with a rabbit anti-COMMD1 pAb (Proteintech Group). Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. * indicates mouse IgG light chain from the antibody used for immunoprecipitation. (C) COMMD1 constructions in pcDNA3.1/Topo plasmid. Two COMMD1 constructs were generated by adding a Myc-tag at the N-terminus of COMMD1: Myc-COMMD1 and a construct with a deletion of the COMM domain named Myc-COMMD1 delta COMM. (D) Representative gels for the same co-immunoprecipitation experiment between COMMD1 and wt- in heterologous system. HeLa cells stably expressing wt- (spTCF-wt) or empty CFTR vector (spTracer) as control were transfected with Myc-COMMD1. spTCF-wt were transfected with Myc-COMMD1 delta COMM. Lysates from all these experiments were subjected to SDS-PAGE, as in (B) after CFTR IP. The 8% SDS-PAGE membrane was probed with anti-CFTR mAb and the 11% SDS-PAGE membrane with anti-c-Myc mAb. Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_19" > <img loading="lazy" src="https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg" alt="Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div></div><div id="ss_next_cav19" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-19" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/cftr-antibody-24-1_mab25031#ReviewsSection"> <div class="sprite_4Stars"></div> (10 Reviews</a>) </div> <div class="tiny"> <a href="/products/cftr-antibody-24-1_mab25031#PublicationSection"> <div class="sprite_pubBook"></div> (76 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IP, WB</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for Calnexin Antibody (NBP1-47546)(3)</h2><div class="ds_info"> <div>We have publications tested in 1 confirmed species: Human.<br /><br />We have publications tested in 1 application: WB.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="WB"> <div style="display:inline;">WB</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">3</span> of 3.<table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using NBP1-47546</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/29093081" target="_blank" rel="nofollow"> Arachchige SP, Henke W, Pramanik A et al. Analysis of Select HSV-1 Proteins for Restriction of Human Immunodeficiency Virus Type 1: The HSV-1 gM Protein Potently Restricts HIV-1 by Preventing the Intracellular Transport and Processing of Env gp160 J. Virol. 2017-11-01 [PMID: 29093081] (Human) </a></td> <td valign="top"></td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td> Kulbay M, Johnson B, Ricaud G et al. DFF40 translocates to the mitochondria during apoptosis and regulates mitochondrial integrity and function Research Square 2021-02-10 </td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35947956" target="_blank" rel="nofollow"> Kim S, Lee K, Choi YS et al. Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development Cell reports 2022-08-09 [PMID: 35947956] (WB)<br /><br />Details:<br />Supplementary Figure S5. </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr></tbody> </table> </div> </div> <a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>Calnexin Antibody (NBP1-47546) (0) </span></h2> <div class="ds_info"> There are no reviews for Calnexin Antibody (NBP1-47546). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. <br/> <ul> <li>Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen</li> <li>Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen</li> </ul> <div class="align-right"> <a href="/reviews/NBP1-47546"><button class="greybutton btn_width" name="novusreview">Submit a Review</button></a><br /> </div> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info">Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/elisa/protocol.html">ELISA</a></li><li><a href="/support/support-by-application/immunocytochemistry/protocol.html">Immunocytochemistry/ Immunofluorescence</a></li><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div><div class="movie-cell"> <span class="movie-title">ICC/IF Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=5AcrhhHmQZs" /> <a href="https://www.youtube.com/embed/5AcrhhHmQZs?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_ICC-IFvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for Calnexin Antibody (NBP1-47546) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a> <a id="positivecontrollysates"></a> <h2 class="greyHead2">Control Lysate(s)</h2> <div class="ds_info"> <table class="wide"><tr class="lined"> <td><a href="hela-lysate_nb800-pc1">HeLa Whole Cell Lysate</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NB800-PC1" data-return="calnexin-antibody-3h4a7_nbp1-47546#supportresearch" data-produrl="calnexin-antibody-3h4a7_nbp1-47546" data-catnums="NB800-PC1" ></div> </td> </tr><tr class="lined"> <td><a href="mcf-7-lysate_nb820-59465">MCF-7 Whole Cell Lysate</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NB820-59465" data-return="calnexin-antibody-3h4a7_nbp1-47546#supportresearch" data-produrl="calnexin-antibody-3h4a7_nbp1-47546" data-catnums="NB820-59465" ></div> </td> </tr> </table> </div><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Mouse&facet_conjugate=HRP">Anti-Mouse HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Mouse&facet_conjugate=FITC">Anti-Mouse FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Mouse&facet_conjugate=Biotin">Anti-Mouse Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Mouse%22">View all of our anti-Mouse Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Mouse%22">Mouse Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional Calnexin Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array NBP1-47546</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/calnexin">Calnexin Antibodies</a></li><li> <a href="/elisa-kits/calnexin">Calnexin ELISA Kits</a></li><li> <a href="/lysates/calnexin">Calnexin Lysates</a></li><li> <a href="/proteins/calnexin">Calnexin Proteins</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr><td valign="top"><h2 class="greyHead2">Research Areas for Calnexin Antibody (NBP1-47546)</h2> <a id="research_areas"></a> <div class="ds_info">Find related products by research area.</div> <div class="ds_info"> <ul class="ds_noDotList"><li class="left width"><a href="/research-areas/cellular-markers/er-markers">ER Markers</a></li><li class="left width"><a href="/research-areas/neuroscience">Neuroscience</a></li><li class="left width"><a href="/research-areas/neuroscience/sensory-systems">Vision</a></li></ul> </div></td></tr></table><h2 class="greyHead2">Blogs on Calnexin. </h2> <a id="blogsect"></a> <div class="ds_info"><a href="/antibody-news/tag/calnexin">Check out the latest blog posts on Calnexin</a>.</div> <div class="ds_info"> <table id="blogs_list_table"> <tbody> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/antibodies/calnexin-an-er-chaperone-that-folds-the-cells-glycoproteins">Calnexin - an ER chaperone that folds the cell's glycoproteins</a></strong><br/> <span>Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  <a href="/antibody-news/antibodies/calnexin-an-er-chaperone-that-folds-the-cells-glycoproteins">Read full blog post.</a></span><br /><br /> </td> </tr></tbody> </table> </div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><div class="datasheet-ads-container" ><a href="https://www.bio-techne.com/resources/blogs/citation-widget-find-products-used-in-literature" target="_blank"><img loading="lazy" src="https://images.novusbio.com/design/citation-app-banner-349x119-v3.jpg" alt="Read our latest blog and use the new citation tool on bio-techne.com" title="new citation tool" class="datasheet-adzone" loading="lazy"></a></div><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="HAF007"> <div class="img_container"> <a href="/products/igg-antibody_haf007" title="Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_HAF007_Western_Blot_19081.jpg" title="Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-antibody_haf007">Goat anti-Mouse IgG Secondary Antibody [HRP]</a> <br />HAF007</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-antibody_haf007';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> <div class="ds_info"> <div id="distributors"></div> </div> <div id="et_reload_trigger" style="display:none;">reload</div><h2 class="greyHead2">Product PDFs</h2><div class="silverHeadShort"> <span class="file"><a href="//aero.bio-techne.com/sds/pdf/nbp1-47546" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//aero.bio-techne.com/sds/pdf/nbp1-47546" target="_blank" class="datasheet-download sdsFile">SDS</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//www.novusbio.com/PDFs/NBP1-47546.pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//www.novusbio.com/PDFs/NBP1-47546.pdf" target="_blank" class="datasheet-download">PDF Datasheet</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="mailto:novus@novusbio.com?subject=Request%20CofA%20for%20NBP1-47546" class="sprite_newDocument"><span class="downloadBlank"> </span></a></span> <span class="download-file-text"><a href="mailto:nb-technical@bio-techne.com?subject=Request%20CofA%20for%20NBP1-47546&body=Specific%20catalog%20number(if multiple sizes):%0ALot%20Number:%0AAny%20additional%20products:">Request CofA</a></span> </div><h2 class="greyHead2">Calculators</h2> <div class="calc_wrapper"> <div class="dsSprite_calc"></div> <div class="fake_link" id="calc_reconstitution_link">Concentration Calculator</div> <div id="calc_reconstitution_wrapper" class="calc_function_wrapper"> <div class="calc_popup"> <div class="calc_close"> <div class="dsSprite_calc_close"></div> </div> <div class="calc_header">Concentration Calculator</div> <div id="reconstitution_calculator" class="calculator"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="concentration" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button"/> </div> </div> </div> </div> </div> <h2 class="greyHead2">Review this Product</h2> <div style="padding:10px;"> Be the first to review our Calnexin Antibody (3H4A7) and receive a gift card or discount.<br /><br /> <div class="text_center"> <div class="dsSprite_stars5" style="position:relative;top:3px;"></div> <a href="/reviews/NBP1-47546"><button class="greybutton btn_width" name="novusreview">Submit a Review</button></a><br /> </div> </div><h2 class="greyHead2">Bioinformatics</h2> <div class="ds_info"> <a id="tab1bioinformatics"></a> <div> <table class="ds_list_right wide top"> <tr class="lined"> <td><strong>Gene Symbol</strong></td> <td><a href="/gene-name/CANX">CANX</a></td> </tr> <tr class="lined"> <td><strong>Entrez</strong></td> <td> <ul class="bioinf-list"><li><a target="_blank" href="//www.ncbi.nlm.nih.gov/gene?term=821" rel="nofollow"><div class="sprite_newWindow"> </div> 821</a><span class="inline ">(Human)</span></li> </ul> </td> </tr><tr class="grey-line-top"> <td><strong>Uniprot</strong></td> 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