{"title":"In vitro Effects of Amygdalin on the Functional Competence of Rabbit Spermatozoa","authors":"Marek Halen\u00e1r, Eva Tvrd\u00e1, Tom\u00e1\u0161 Slanina, \u013dubom\u00edr Ondru\u0161ka, Eduard Koles\u00e1r, Peter Mass\u00e1nyi, Adriana Koles\u00e1rov\u00e1 ","volume":119,"journal":"International Journal of Animal and Veterinary Sciences","pagesStart":712,"pagesEnd":717,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/10005743","abstract":"The present <em>in vitro<\/em> study was designed to reveal whether amygdalin (AMG) is able to cause changes to the motility, viability and mitochondrial activity of rabbit spermatozoa. New Zealand White rabbits (n = 10) aged four months were used in the study. Semen samples were collected from each animal and used for the <em>in vitro <\/em>incubation. The samples were divided into five equal parts and diluted with saline supplemented with 0, 0.5, 1, 2.5 and 5 mg\/mL AMG. At times 0h, 3h and 5h spermatozoa motion parameters were assessed using the SpermVision&trade; computer-aided sperm analysis (CASA) system, cell viability was examined with the metabolic activity (MTT) assay, and the eosin-nigrosin staining technique was used to evaluate the viability of rabbit spermatozoa. All AMG concentrations exhibited stimulating effects on the spermatozoa activity, as shown by a significant preservation of the motility (P&lt;0.05 with respect to 0.5 mg\/mL and 1 mg\/mL AMG; Time 5 h) and mitochondrial activity (P&lt;&thinsp;0.05 in case of 0.5 mg\/mL AMG; P&lt;&thinsp;0.01 in case of 1 mg\/mL AMG; P&thinsp;&lt;&thinsp;0.001 with respect to 2.5 mg\/mL and 5 mg\/mL AMG; Time 5 h). None of the AMG doses supplemented had any significant impact of the spermatozoa viability. 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