Comprehensive body fluid identification and contributor assignment by combining targeted sequencing of mRNA and coding region SNPs

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<ul class="nav navbar-nav navbar-right" style="display: inline-flex; align-items: center; margin-left: 20px;"> <li id="language" class="d-none d-xl-inline-flex"> <a href="javascript:"> <div class="current"> <i class="ivu-icon ivu-icon-md-globe"></i> EN </div> </a> <div class="selection"> <a rel="alternate" hreflang="en" href="https://www.peeref.com/works/83754813" > <span>English</span> </a> <a rel="alternate" hreflang="zh" href="https://www.peeref.com/zh/works/83754813" > <span>中文</span> </a> </div> </li> </ul> </ul> </div> </nav> <main> <div id="top-info-banner" class="container-fluid mb-0"> <div class="container"> <div class="d-flex align-items-center" style="margin-top: 30px;"> <span class="text-white"> <strong class="f18">☆</strong> <span class="f16">4.5</span> </span> <span class="mx-3"></span> <span class="tag">Article</span> </div> <h1 class="title title-for-article"> Comprehensive body fluid identification and contributor assignment by combining targeted sequencing of mRNA and coding region SNPs </h1> <div class="help-links-left"> <p class="pub-info"> FORENSIC SCIENCE INTERNATIONAL-GENETICS (2024) </p> </div> </div> </div> <div id="article-sticky-navbar"> <div class="container"> <div class="d-flex justify-content-between flex-wrap flex-md-nowrap"> <div class="d-flex align-items-center mb-2"> <ul class="nav nav-underline f16 font-weight-bold"> <li class="active"> <a href="javascript:;"> Overview </a> </li> <li class=""> <a href="https://www.peeref.com/works/83754813/comments"> Write a Review </a> </li> </ul> </div> <div class="d-flex align-items-center justify-content-md-end flex-wrap flex-md-nowrap"> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <a href="https://doi.org/10.1016/j.fsigen.2024.103125" target="_blank" class="btn btn-warning btn-circle"> <i class="ivu-icon ivu-icon-md-copy f16"></i> <strong>Get Full Text</strong> </a> </div> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <a href="https://www.peeref.com/works/83754813/add-to-collection" class="btn 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</div> </div> </div> </div> </div> </div> <div id="article-details" class="container"> <div class="col-md-4 px-0 pr-md-3"> <div class="f15 panel-box rounded shadow-none border"> <div class="mb-3 pb-3"> <h4 class="mt-0">Journal</h4> <div class="f16"> <h5 class="title f16"> <a href="https://www.peeref.com/journals/2908/forensic-science-international-genetics"> FORENSIC SCIENCE INTERNATIONAL-GENETICS </a> </h5> <span> Volume 73, Issue -, Pages - </span> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Publisher</h4> <div class="f16"> <h5 class="title f16 text-primary"> ELSEVIER IRELAND LTD </h5> <div class="my-2"> DOI: 10.1016/j.fsigen.2024.103125 </div> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Keywords</h4> <div class="f16"> Forensic RNA analysis; Massively parallele sequencing (MPS); Donor assignment; Biological trace contextualization; Source level, activity level </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Categories</h4> <div class="f16"> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Genetics+%26+Heredity" target="_blank" class="text-dark btn btn-link p-0 text-left"> Genetics & Heredity </a> </span> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Medicine%2C+Legal" target="_blank" class="text-dark btn btn-link p-0 text-left"> Medicine, Legal </a> </span> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Funding</h4> <div class="f16"> <ol class=""> <li>EU Internal Se-curity Fund [IZ25-5793-2019-36]</li> </ol> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 text-center">Ask authors/readers for more resources</h4> <div class="requests"> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/file.png" alt=""> </div> <h4>Protocol</h4> <p> <a href="https://www.peeref.com/works/83754813/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/experiment.png" alt=""> </div> <h4>Reagent</h4> <p> <a href="https://www.peeref.com/works/83754813/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> </div> </div> </div> <div class="col-md-8 px-0 pl-md-3"> <div id="article-summary-panel" class="mb-4"> <ul class="nav nav-tabs" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#ai_summary" data-toggle="tab" class="summary-tab mx-0 f16 text-dark"> <strong>Automated Summary</strong> <strong class="text-danger ml-1"><i>New</i></strong> </a> </li> <li class=""> <a href="#raw_abstract" data-toggle="tab" class="abstract-tab mx-0 f16 text-dark"> <strong>Abstract</strong> </a> </li> </ul> <div class="tab-content border border-top-0"> <div id="ai_summary" class="tab-pane active"> <div class="summary-panel panel-box mb-0 rounded shadow-none"> <div class="f16">This article describes the development of an mRNA/cSNP panel for forensic genetic analysis, which can provide crucial contextualizing information on the source level about a trace's composition and reduce the risk of association fallacies by typing individual coding region SNPs. The panel has high reliable detection sensitivity and low RNA input, and further optimization and improvement are needed in the future.</div> </div> </div> <div id="raw_abstract" class="tab-pane "> <div class="abstract-panel panel-box mb-0 rounded shadow-none"> <div class="f16">Forensic genetic analyses aim to retrieve as much information as possible from biological trace material recovered from crime scenes. While standard short tandem repeat (STR) profiling is essential to individualize biological traces, its significance is diminished in crime scenarios where the presence of a suspect's DNA is acknowledged by all parties. In such cases, forensic (m)RNA analysis can provide crucial contextualizing information on the source level about a trace's composition, i.e., body fluids/tissues, and has therefore emerged as a powerful tool for modern forensic investigations. However, the question which of several suspects contributed a specific component (body fluid) to a mixed trace cannot be answered by RNA analysis using conventional methods. This individualizing information is stored within the sequence of the mRNA transcripts. Massively parallel sequencing (MPS) represents a promising alternative, offering not only higher multiplex capacity, but also the typing of individual coding region SNPs (cSNPs) to enable the assignment of contributors to mixture components, thereby reducing the risk of association fallacies. Herein, we describe the development of an extensive mRNA/cSNP panel for targeted sequencing on the IonTorrent S5 platform. Our panel comprises 30 markers for the detection of six body fluids/tissues (blood, saliva, semen, skin, vaginal and menstrual secretion), along with 70 linkage-controlled cSNPs for contributor assignment. It exhibited high reliable detection sensitivity with RNA inputs down to 0.75 ng and a conservatively calculated probability of identity of 0.03 - 6 % for individual body fluid-specific cSNP profiles. Limitations and areas for future work include RNA-related allele imbalances, inclusion of markers to correctly identify rectal mucosa and the optimization of specific markers. In summary, our new panel is intended to be a major step forward to interpret biological evidence at sub-source and source level based on cSNP attribution of a body fluid component to a suspect and victim, respectively.</div> </div> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Authors</h4> <div class="mb-3"> <article-authors tid="83754813" list="[{"name":"Maximilian Neis","sequence":1},{"name":"Theresa Gross","sequence":2},{"name":"Harald Schneider","sequence":3},{"name":"Peter M. Schneider","sequence":4},{"name":"Cornelius Courts","sequence":5}]" verified="[]" page="work" ></article-authors> </div> <div class="alert alert-warning mb-0"> <h5 class="mt-0 bg-warning text-dark px-3 rounded d-inline-block"> I am an author on this paper </h5> <div class="font-weight-bold f13"> Click your name to claim this paper and add it to your profile. </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Reviews</h4> <div class="d-flex flex-wrap flex-md-nowrap"> <div class="flex-grow-1"> <h4 class="f16"> Primary Rating <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="The primary rating indicates the level of overall quality for the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap align-items-center alert mb-0"> <div class="d-flex align-items-center justify-content-center"> <Rate disabled allow-half value="4.5" style="font-size: 28px;"></Rate> <strong class="f20 m-3" style="color: #f5a623;">4.5</strong> </div> <div class="text-muted mx-4"> Not enough ratings </div> </div> <h4 class="f16"> Secondary Ratings <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="Secondary ratings independently reflect strengths or weaknesses of the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap alert"> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Novelty</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Significance</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Scientific rigor</h5> <strong class="mx-4">-</strong> </div> </div> </div> <div class="flex-shrink-0"> <div class="border bg-light py-2 px-4"> <h5 class="mb-1">Rate this paper</h5> <Rate class="f24" @on-change="function(value){ location.href='https://www.peeref.com/works/83754813/comments?rating='+value }"></Rate> </div> </div> </div> </div> <div id="collection" class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Recommended</h4> <div class="my-3"> <ul class="nav nav-pills border-bottom pb-3" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#articles_from_related" data-toggle="tab" class="mx-0 f15"> <strong>Related</strong> </a> </li> <li class=""> <a href="#articles_from_authors" data-toggle="tab" class="mx-0 f15"> <strong>From Same Authors</strong> </a> </li> <li class=""> <a href="#articles_from_journal" data-toggle="tab" class="mx-0 f15"> <strong>From Same Journal</strong> </a> </li> </ul> <div class="tab-content"> <div id="articles_from_related" class="tab-pane active"> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/33746848" class="text-dark hover-underline">Selecting mRNA markers in blood for age estimation of the donor of a biological stain</a> </h4> <p class="text-ellipsis-2">Guro Dorum, Nadescha Viviane Haenggi, Dario Burri, Yael Marti, Regine Banemann, Galina Kulstein, Cornelius Courts, Annica Gosch, Thorsten Hadrys, Cordula Haas, Jacqueline Neubauer</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2908.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> RNA has gained attention in forensic field for predicting biological age. 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Lewis, Erin Weaver, Audrey Hoyle, Robert Lagace, Fabio Oldoni, Daniele Podini</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/3787.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Genotype profiling is crucial in forensics, with advancements in technology necessitating re-analysis of DNA data from the 1990s to overcome potential confounding factors like paralogous genes. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF LEGAL MEDICINE</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/22549761/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/26836966" class="text-dark hover-underline">Routine Mitogenome MPS Analysis from 1 and 5 mm of Rootless Human Hair</a> </h4> <p class="text-ellipsis-2">Lauren. C. C. Canale, Jennifer. A. A. McElhoe, Gloria Dimick, Katherine. M. M. DeHeer, Jason Beckert, Mitchell. M. M. Holland</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/9770.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigates the impact of hair age and physical characteristics on mtDNA recovery and MPS data quality. The results show that reliable mitogenome sequences can be generated from 1 and 5 mm hair shaft samples up to 27 years of age using MPS. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">GENES</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26836966/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/82378666" class="text-dark hover-underline">Utilizing Massively Parallel Sequencing (MPS) of Human Leukocyte Antigen (HLA) Gene Polymorphism to Assess Relatedness in Deficiency Parentage Testing</a> </h4> <p class="text-ellipsis-2">Diamanto I. Kouniaki, Konstantinos V. Fotopoulos, Katerina Tarassi, Alexandra Tsirogianni</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/9770.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study evaluated the potential use of HLA gene polymorphism as a forensic marker for paternity testing using MPS technology. It found that HLA genes significantly enhanced kinship determination and had advantages over aSTR analysis. The study also emphasized the importance of comprehensive reference databases and high-resolution HLA typing, and pointed out the potential of reintegrating HLA alleles into forensic identification. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">GENES</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82378666/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/28372745" class="text-dark hover-underline">TrACES of time: Transcriptomic analyses for the contextualization of evidential stains - Identification of RNA markers for estimating time-of-day of bloodstain deposition</a> </h4> <p class="text-ellipsis-2">A. Gosch, A. Bhardwaj, C. Courts</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2908.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study identified candidate markers for predicting the time of day of sample deposition in forensic molecular biology. The use of sequencing technologies and statistical methods showed potential in delivering relevant contextualizing information. There was notable variation between donors and storage conditions, highlighting the importance of accurate quantification methods and robust normalization procedures. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28372745/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/24609583" class="text-dark hover-underline">Pairwise kinship analysis of 17 pedigrees using massively parallel sequencing</a> </h4> <p class="text-ellipsis-2">Ruiyang Tao, Qiannan Xu, Shouyu Wang, Ruocheng Xia, Qi Yang, Anqi Chen, Yiling Qu, Yehui Lv, Suhua Zhang, Chengtao Li</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2908.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> With the development of massively parallel sequencing (MPS), the kinship analysis has become more convenient. In this study, 243 Chinese Han individuals from 17 families were sequenced using the ForenSeq Signature Kit, and the analysis was conducted using likelihood ratio and identical by state methods. The results showed that the kit was effective in analyzing parent-child, full sibling, and most second-degree kinships. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/24609583/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/27757489" class="text-dark hover-underline">An MPS-Based 50plex Microhaplotype Assay for Forensic DNA Analysis</a> </h4> <p class="text-ellipsis-2">Ranran Zhang, Jiaming Xue, Mengyu Tan, Dezhi Chen, Yuanyuan Xiao, Guihong Liu, Yazi Zheng, Qiushuo Wu, Miao Liao, Meili Lv, Shengqiu Qu, Weibo Liang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/9770.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, a panel of 50 Microhaplotypes (MHs) was constructed and analyzed using the Multiseq multiple polymerase chain reaction (multi-PCR) targeted capture sequencing protocol. It was found that this panel has the advantages of both short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs), with high sensitivity and specificity. It also showed measurable polymorphism among a group of Han individuals and had high detection rates for highly degraded samples and mixtures. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">GENES</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/27757489/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/28372724" class="text-dark hover-underline">Targeted DNA methylation analysis and prediction of smoking habits in blood based on massively parallel sequencing</a> </h4> <p class="text-ellipsis-2">Athina Vidaki, Benjamin Planterose Jimenez, Brando Poggiali, Vivian Kalamara, Kristiaan J. van der Gaag, Silvana C. E. Maas, Mohsen BIOS Consortium, Mohsen Ghanbari, Titia Sijen, Manfred Kayser</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2908.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Tobacco smoking is a widespread habit with significant health risks and mortality. This study aims to predict smoking habits using blood DNA methylation. The results show high reproducibility of methylation measurements and accurate prediction of smoking habits using corrected data. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28372724/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/81916018" class="text-dark hover-underline">Multi-System-Level Analysis with RNA-Seq on Pterygium Inflammation Discovers Association between Inflammatory Responses, Oxidative Stress, and Oxidative Phosphorylation</a> </h4> <p class="text-ellipsis-2">Ye-Ah Kim, Yueun Choi, Tae Gi Kim, Jisu Jeong, Sanghyeon Yu, Taeyoon Kim, Kisung Sheen, Yoonsung Lee, Taesoo Choi, Yong Hwan Park, Min Seok Kang, Man S. Kim</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/3812.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study collected RNA-seq from pterygial and conjunctival tissues and compared Asian and European pterygiums. 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Jones, Eleanor R. Schrems, Ruqaiza Muhyudin, Tyrone A. Washington, Kevin A. Murach, Nicholas P. Greene</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/453.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study is the first to integrate and compare the transcriptional landscape of skeletal muscle across multiple preclinical models and biological sexes. We highlight that 1) two-thirds of the transcriptional changes in early CC are conserved at the late stage, 2) DEGs are largely model and sex specific, and 3) transcriptional factors, including CLOCK and ARNTL/BMAL1, which influence the gene expression in early CC, may represent a global therapeutic target with the potential for efficacy across various cancer types. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84519407/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Dermatology </span> </div> <h4> <a href="https://www.peeref.com/works/28362381" class="text-dark hover-underline">Single-cell analysis of human dermal fibroblasts isolated from a single male donor over 35?years</a> </h4> <p class="text-ellipsis-2">Eriko Itai, Toru Atsugi, Ken Inomata, Mika Yamashita, Kazuhiko Kaji, Daisuke Nanba, Eiji Naru</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2764.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The aim of this study was to investigate the impact of aging on dermal fibroblast heterogeneity using samples from the same donor. The findings showed that as the donor aged, dermal fibroblasts exhibited reduced proliferation and acquired senescent traits. Transcriptome analysis revealed downregulation of genes associated with extracellular matrix remodeling with aging. This study provides valuable insights into the behavior of dermal fibroblasts during skin aging. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">EXPERIMENTAL DERMATOLOGY</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28362381/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/26332208" class="text-dark hover-underline">TrACES of time: Transcriptomic Analyses for the Contextualization of Evidential Stains-towards estimating the time of deposition</a> </h4> <p class="text-ellipsis-2">A. Gosch, A. Bhardwaj, C. Courts</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/22411.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, we aim to predict the time of day of biological trace deposition using transcriptomic analyses and evaluate its potential. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26332208/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/33641912" class="text-dark hover-underline">Identification of feature genes and key biological pathways in immune-mediated necrotizing myopathy: High-throughput sequencing and bioinformatics analysis</a> </h4> <p class="text-ellipsis-2">Kai Chen, Chun-yan Zhu, Jia-ying Bai, Feng Xiao, Song Tan, Qiao Zhou, Li Zeng</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/10376.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated the potential mechanisms and key biomarkers of immune-mediated necrotizing myopathy (IMNM) using high-throughput sequencing and bioinformatics technology. LTK, MYBPH, and MYL4 were identified as potential key molecules for IMNM, which may play a role in the autophagy-lysosome pathway and muscle inflammation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/33641912/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> <div id="articles_from_authors" class="tab-pane "> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/84852639" class="text-dark hover-underline">Shedding the status-a meta-analysis on the individual propensity to shed skin material in the context of forensic trace analysis</a> </h4> <p class="text-ellipsis-2">D. Koch, A. Gosch, C. Courts</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The individual shedding propensity (ISP) is an important topic in forensic genetic research. However, the research results on its existence, influencing factors, classifications, and test methods are inconsistent and insufficiently documented. Through a systematic review and meta-analysis of 50 relevant publications, it was found that the shedding propensity is a genuine and determinable individual variable, influenced by gender and age, but not by handwashing time or hand selection. It is recommended to set a standardized contact time, use plastic tubes as the contact surface or perform direct skin abrasion in testing, and consider the influence of body region. The results also emphasize the importance of coordinating and standardizing research designs and experiments, and suggest conducting quality control before publishing data. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF LEGAL MEDICINE</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84852639/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/82210007" class="text-dark hover-underline">Evolutionary trajectories of small cell lung cancer under therapy</a> </h4> <p class="text-ellipsis-2">Julie George, Lukas Maas, Nima Abedpour, Maria Cartolano, Laura Kaiser, Rieke N. Fischer, Andreas H. Scheel, Jan-Philipp Weber, Martin Hellmich, Graziella Bosco, Caroline Volz, Christian Mueller, Ilona Dahmen, Felix John, Cleidson Padua Alves, Lisa Werr, Jens Peter Panse, Martin Kirschner, Walburga Engel-Riedel, Jessica Juergens, Erich Stoelben, Michael Brockmann, Stefan Grau, Martin Sebastian, Jan A. Stratmann, Jens Kern, Horst-Dieter Hummel, Balazs Hegedues, Martin Schuler, Till Ploenes, Clemens Aigner, Thomas Elter, Karin Toepelt, Yon-Dschun Ko, Sylke Kurz, Christian Grohe, Monika Serke, Katja Hoepker, Lars Hagmeyer, Fabian Doerr, Khosro Hekmath, Judith Strapatsas, Karl-Otto Kambartel, Geothy Chakupurakal, Annette Busch, Franz-Georg Bauernfeind, Frank Griesinger, Anne Luers, Wiebke Dirks, Rainer Wiewrodt, Andrea Luecke, Ernst Rodermann, Andreas Diel, Volker Hagen, Kai Severin, Roland T. Ullrich, Hans Christian Reinhardt, Alexander Quaas, Magdalena Bogus, Cornelius Courts, Peter Nuernberg, Kerstin Becker, Viktor Achter, Reinhard Buettner, Juergen Wolf, Martin Peifer, Roman K. Thomas</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study used multi-region sequencing of tumors from patients with small cell lung cancer to uncover the evolutionary processes of tumors during treatment. It was found that tumors before treatment showed clonal homogeneity, while chemotherapy led to an increase in genomic intratumor heterogeneity and spatial clonal diversity. After treatment, tumor relapse was associated with a shift in ancestral clones, and radiotherapy or immunotherapy could induce the re-expansion of founder clones. In addition, gene alterations and genomic patterns associated with disease relapse and chemotherapy resistance were also identified. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NATURE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82210007/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/82628898" class="text-dark hover-underline">Forensically relevant anatomical brain regions cannot be sub-differentiated by RNA expression analysis</a> </h4> <p class="text-ellipsis-2">Jan Euteneuer, Lucas Moitinho-Silva, Cornelius Courts</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study aimed to identify mRNA candidates for differentiating between different regions of the cerebral cortex, but the results showed that the differences in mRNA expression between these regions are too small to be useful for forensic RNA analysis. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE MEDICINE AND PATHOLOGY</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82628898/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, General & Internal </span> </div> <h4> <a href="https://www.peeref.com/works/82928551" class="text-dark hover-underline">RNA Analysis in Forensic Molecular Biology</a> </h4> <p class="text-ellipsis-2">Cornelius Courts, Annica Gosch, Markus Rothschild</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This article describes the important role of RNA in cells and tissues, and the method of analyzing the transcriptome to determine the type and state of cells. It also discusses the application of this method in the forensic field, including determining the tissue type and composition of mixed traces in biological samples, and obtaining information related to crimes. Finally, it is pointed out that forensic RNA analysis has great potential and may be applied in more forensic cases in the future. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">DEUTSCHES ARZTEBLATT INTERNATIONAL</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82928551/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/28372745" class="text-dark hover-underline">TrACES of time: Transcriptomic analyses for the contextualization of evidential stains - Identification of RNA markers for estimating time-of-day of bloodstain deposition</a> </h4> <p class="text-ellipsis-2">A. Gosch, A. Bhardwaj, C. Courts</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study identified candidate markers for predicting the time of day of sample deposition in forensic molecular biology. The use of sequencing technologies and statistical methods showed potential in delivering relevant contextualizing information. There was notable variation between donors and storage conditions, highlighting the importance of accurate quantification methods and robust normalization procedures. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28372745/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/28432222" class="text-dark hover-underline">Spitting in the wind?-The challenges of RNA sequencing for biomarker discovery from saliva</a> </h4> <p class="text-ellipsis-2">Annica Gosch, Regine Banemann, Guro Dorum, Cordula Haas, Thorsten Hadrys, Nadescha Haenggi, Galina Kulstein, Jacqueline Neubauer, Cornelius Courts</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study examines the challenges in discovering forensic biomarkers for contextualization in saliva samples, such as the presence of oral bacteria, sample heterogeneity, and degradation. Recommendations are provided to improve RNA biomarker discovery. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF LEGAL MEDICINE</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28432222/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/21383389" class="text-dark hover-underline">mRNA profiling of mock casework samples: Results of a FoRNAP collaborative exercise</a> </h4> <p class="text-ellipsis-2">Andrea Patrizia Salzmann, Malte Bamberg, Cornelius Courts, Guro Dorum, Annica Gosch, Thorsten Hadrys, Gavrilo Hadzic, Maximilian Neis, Peter M. Schneider, Titia Sijen, Margreet van den Berge, Peter Wiegand, Cordula Haas</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Forensic mRNA profiling is increasingly being used to identify the origin of human body fluids, with different extraction and analysis methods showing potential in casework analysis. High input samples are accurately typed by all laboratories, but low input or mixed stains present challenges in analysis. efforts to optimize analysis and interpretation strategies may be desirable for achieving consistency in interpretation results. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2021) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/21383389/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/21759256" class="text-dark hover-underline">Nothing but hot air?-On the molecular ballistic analysis of backspatter generated by and the hazard potential of blank guns</a> </h4> <p class="text-ellipsis-2">Jan Euteneuer, Annica Gosch, Cornelius Courts</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, backspatter generated by shots from blank cartridge guns on biological targets was investigated and found to be present in all cases with skin simulants being penetrated and backspatter generated. This demonstrates the significant hazard potential of these weapons and the importance of proper analysis and recovery of trace material. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF LEGAL MEDICINE</span> (2021) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/21759256/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/21759263" class="text-dark hover-underline">Ten years of molecular ballistics-a review and a field guide</a> </h4> <p class="text-ellipsis-2">Jan Euteneuer, Cornelius Courts</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Molecular ballistics combines insights and approaches from molecular biology, forensic ballistic, and wound ballistic to describe and analyze the complex patterns of biological evidence generated by gunshots at biological targets. It has covered a lot of ground since its inception in 2010, intersecting with other forensic disciplines and aims to raise awareness about the sources of forensic evidence from backspatter traces and the inside surfaces of firearms. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF LEGAL MEDICINE</span> (2021) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/21759263/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/20984657" class="text-dark hover-underline">DNA transfer to firearms in alternative realistic handling scenarios</a> </h4> <p class="text-ellipsis-2">Annica Gosch, Jan Euteneuer, Johanna Preuss-Woeessner, Cornelius Courts</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2020) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/20984657/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/20588908" class="text-dark hover-underline">A distant relationship?-investigation of correlations between DNA isolated from backspatter traces recovered from firearms, wound profile characteristics, and shooting distance</a> </h4> <p class="text-ellipsis-2">Jan Euteneuer, Annica Gosch, Philipp Cachee, Cornelius Courts</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF LEGAL MEDICINE</span> (2020) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/20588908/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/17368845" class="text-dark hover-underline">All mixed up?-genotype change after stem cell transplantation impeded verification of 21-year-old semen sample-a case report</a> </h4> <p class="text-ellipsis-2">Cornelius Courts, Johanna Preuss-Woessner</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF LEGAL MEDICINE</span> (2019) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/17368845/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Editorial Material </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/18384545" class="text-dark hover-underline">There is no evidence that dogs can smell DNA - Comment on Individual human scent as a forensic identifier using mantrailing</a> </h4> <p class="text-ellipsis-2">Cornelius Courts, Jan Euteneuer, Annica Gosch</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL</span> (2019) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/18384545/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/18384641" class="text-dark hover-underline">On DNA transfer: The lack and difficulty of systematic research and how to do it better</a> </h4> <p class="text-ellipsis-2">Annica Gosch, Cornelius Courts</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2019) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/18384641/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> <div id="articles_from_journal" class="tab-pane "> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83039808" class="text-dark hover-underline">Application of a newly constructed NGS panel with 45 X-linked microhaplotypes demonstrates the unique value of X-MH for kinship testing and mixture analysis</a> </h4> <p class="text-ellipsis-2">Guanju Ma, Kailiang Liu, Chaolong Lu, Qingqing Du, Mengjie Zhang, Qian Wang, Guangping Fu, Junyan Wang, Chunling Ma, Bin Cong, Shujin Li, Lihong Fu</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study screened 63 X-MHs, evaluated their performance, and calculated population parameters. The panel performed well in personal identification and paternity testing, and showed unique advantages in complex kinship and male DNA mixture analyses. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83039808/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84361787" class="text-dark hover-underline">CRISPR-Cas technology in forensic investigations: Principles, applications, and ethical considerations</a> </h4> <p class="text-ellipsis-2">Ana Filipa Sobral, Ricardo Jorge Dinis-Oliveira, Daniel Jose Barbosa</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> CRISPR-Cas systems are adaptive immune systems in bacteria, and CRISPR-Cas-based technologies can be used for precise genome editing. Forensic science is used to investigate and solve legal issues, and CRISPR-Cas-based methods have potential applications in the field of forensic science, but also raise ethical concerns and potential abuse of personal genetic information. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84361787/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83532016" class="text-dark hover-underline">Dense SNP-based analyses complement forensic anthropology biogeographical ancestry assessments</a> </h4> <p class="text-ellipsis-2">Sammed N. Mandape, Bruce Budowle, Heather McKiernan, Donia Slack, Sarah Mittelman, Kristen Mittelman, David Mittelman</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Forensic anthropological examinations can estimate the ancestry of human remains, but they have limitations. Genome-wide SNP testing provides a more accurate method for ancestry estimation. The study found inconsistencies between anthropological and genomic ancestry estimates, especially for admixed populations. Further validation studies and policy improvements are crucial for improving the accuracy of ancestry estimation and reducing misinformation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83532016/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83533129" class="text-dark hover-underline">Shotgun DNA sequencing for human identification: Dynamic SNP selection and likelihood ratio calculations accounting for errors</a> </h4> <p class="text-ellipsis-2">Mikkel Meyer Andersen, Marie-Louise Kampmann, Alberte Honore Jepsen, Niels Morling, Poul Svante Eriksen, Claus Bursting, Jeppe Dyrberg Andersen</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This paper presents a statistical model for human identification using shotgun sequencing data, which takes into account the sequencing error rate and can be applied to highly degraded low-quality DNA samples. An open-source R package, wgsLR, is also introduced for implementing the method. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83533129/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84342778" class="text-dark hover-underline">SMART: STR Mixture Analysis and Resolution Tools</a> </h4> <p class="text-ellipsis-2">Xianchao Ji, Lianjiang Chi, Lan Wu Chen, Jianchao Chen, Anxin Yan, Yongjiu Li, Zheng Tu, Jian Ye, Hua Chen</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This article presents SMART, a software for analyzing STR mixture profiles developed within the fully continuous model framework. SMART integrates multiple models and offers various functions, such as likelihood ratio calculation, genotype resolution, and database search. Its performance was evaluated using laboratory samples and the PROVEDIt dataset, showing high sensitivity, specificity, and precision, as well as computational efficiency. SMART is expected to be a valuable tool in forensic investigations, improving the accuracy and reliability of criminal justice outcomes. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84342778/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83225861" class="text-dark hover-underline">Profiling age and body fluid DNA methylation markers using nanopore adaptive sampling</a> </h4> <p class="text-ellipsis-2">Zaka Wing-Sze Yuen, Somasundhari Shanmuganandam, Maurice Stanley, Simon Jiang, Nadine Hein, Runa Daniel, Dennis McNevin, Cameron Jack, Eduardo Eyras</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> DNA methylation plays a crucial role in physiological processes and can be used as a biomarker for body fluid identification and age prediction. Current methylation detection methods rely on various techniques and markers, requiring specialized DNA preparation and biochemical treatments. This study used nanopore adaptive sampling technology to simultaneously identify age-associated and body fluid-specific methylation markers without the need for specialized DNA preparation or biochemical treatments. The technology was consistent with whole-genome bisulfite sequencing data and identified new sites strongly correlated with age. This study lays the foundation for the development of nanopore-based methods for age prediction and body fluid identification. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83225861/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83599477" class="text-dark hover-underline">The role of cats in human DNA transfer</a> </h4> <p class="text-ellipsis-2">Heidi Monkman, Roland A. H. van Oorschot, Mariya Goray</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated whether cats are reservoirs and vectors for human DNA transfer. The results showed that human DNA was prevalent on all cats, and its distribution followed a certain pattern. In addition, short-term patting contact could lead to the transfer of human DNA to cats. These findings suggest that we should consider the role of animals in DNA reporting and collect DNA evidence from animals in cases involving interactions between animals and perpetrators. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83599477/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84866026" class="text-dark hover-underline">Uncovering genetic signatures of the Walser migration in the Alps: Patterns of diversity and differentiation</a> </h4> <p class="text-ellipsis-2">Peter Resutik, Joelle Schneider, Simon Aeschbacher, Magnus Dehli Vigeland, Mario Gysi, Corinne Moser, Chiara Barbieri, Paul Widmer, Mathias Currat, Adelgunde Kratzer, Michael Kruetzen, Cordula Haas, Natasha Arora</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated the genetic diversity and differentiation of the Walser people. By comparing samples from Walser, Walser-homeland, and non-Walser Alpine communities, as well as an idealized Swiss reference population, it was found that Walser-homeland and Walser communities showed low to moderate genetic differentiation from other communities, with more remote communities showing stronger differentiation. Additionally, the rare haplogroup W6 was identified in the Walser communities. This study contributes to understanding the genetic characteristics of the Walser people, but also highlights the need for more comprehensive research. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84866026/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84265916" class="text-dark hover-underline">Towards the identification of body fluids using RT-LAMP isothermal amplification coupled with CRISPR-Cas12a</a> </h4> <p class="text-ellipsis-2">Courtney R. H. Lynch, Olivia L. Martin, Craig Billington, Rachel Fleming</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This paper describes a new technique for the detection of body fluids that combines RT-LAMP amplification with CRISPR-mediated fluorescent detection to rapidly detect mRNA markers in body fluids at a single temperature. The technique has high sensitivity and specificity and can be used for body fluid identification in forensic screening laboratories. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84265916/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/85230017" class="text-dark hover-underline">The ReAct project: Analysis of data from 23 different laboratories to characterise DNA recovery given two sets of activity level propositions</a> </h4> <p class="text-ellipsis-2">Peter Gill, Ane Elida Fonnelop, Tacha Hicks, Stavroulla Xenophontos, Marios Cariolou, Roland van Oorschot, Iris Buckel, Viktorija Sukser, Suncica Papic, Sinisa Merkas, Ana Kostic, Angela Marques Pereira, Christina Teutsch, Christina Forsberg, Cordula Haas, Elizabet Petkovski, Fabian Hass, Jan Masek, Jelena Stosic, Yong Sheng Lee, Christopher Kiu-Choong Syn, Linda Groombridge, Marc Trimborn, Marilena Hadjivassiliou, Michelle Breathnach, Jana Novackova, Walther Parson, Petra Hatzer-Grubwieser, Sanna Pietikainen, Simone Joas, Sascha Willuweit, Stefanie Grethe, Tamara Milicevic, Therese Hasselqvist, Venus Kallupurackal, Vlastimil Stenzl, Staffan Jansson, Ingrun Glocker, Sarah Brunck, Karoline Nyhagen, Anne Berit Dyve Lingelem, Heli Autere, Devon Thornbury, Natalie Pedersen, Stephanie Fox, David Moore, Gemma Escott, Cathrine Bie Petersen, Hans Jakob Larsen, Rebecca Giles, Paul Stafford Allen, Lourdes Prieto, Ester Ramirez, Isabel Martinez de Yuso, Ingo Bastisch</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The project consists of multiple laboratories aiming to simulate typical casework situations, collect data, and implement Bayesian networks to assess the value of DNA results. Two different transfer methods were used in the experiment. The study found that in the direct transfer experiment, the results could not distinguish between propositions unless a single contributor profile matching the known person of interest was retrieved. In the indirect transfer experiment, both single and major contributor profiles matching the person of interest supported the proposition that the person used the tool. The study also emphasized the importance of standardizing methods and developing new approaches to assign the probability of laboratory-dependent DNA recovery, and the crucial role of freely accessible open databases in supporting these efforts. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/85230017/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83112863" class="text-dark hover-underline">Improved individual identification in DNA mixtures of unrelated or related contributors through massively parallel sequencing</a> </h4> <p class="text-ellipsis-2">Zhiyong Liu, Enlin Wu, Ran Li, Jiajun Liu, Yu Zang, Bin Cong, Riga Wu, Bo Xie, Hongyu Sun</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study evaluated the impact of potential kinship on individual identification, including MPS performance, the influence of genetic markers on kinship and NOC inference, the probability distribution of MAC and TAC, trends in LR values, and comparisons of length- and sequence-based STR genotypes. Results showed that multiple genetic markers improved the accuracy of kinship and NOC inference, the LR value of the POI depended on the mixing ratio, and the correct kinship hypothesis yielded more conservative LR values. In addition, using sequence-based STR genotypes increased the power of individual identification and the accuracy of mixture ratio inference. The MGIEasy Signature Identification Library Prep kit demonstrated robust individual identification capabilities and is suitable for forensic DNA mixture interpretation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83112863/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83911917" class="text-dark hover-underline">A preliminary study on detecting human DNA in aquatic environments: Potential of eDNA in forensics</a> </h4> <p class="text-ellipsis-2">Marie Antony Dass, Craig D. H. Sherman, Roland A. H. van Oorschot, Dadna Hartman, Gemma Carter, Annalisa Durdle</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated the detection and degradation rates of human eDNA in saltwater and freshwater samples, as well as the recovery of STR profiles and mtDNA sequencing. Results showed that human eDNA could be detected for up to 36-84 hours in water samples, with a detection limit of 205 copies/μl. Partial STR profiles could be recovered within 24 hours, and full mtDNA profiles could be recovered from freshwater samples within 48 hours and remained detectable for up to 72 hours in saltwater. This study emphasizes the importance of considering and incorporating human eDNA analysis in forensic practice. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83911917/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84482295" class="text-dark hover-underline">Benchmarking for genotyping and imputation using degraded DNA for forensic applications across diverse populations</a> </h4> <p class="text-ellipsis-2">Elena I. Zavala, Rori V. Rohlfs, Priya Moorjani</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated computational methods for handling degraded DNA in forensic applications. By simulating sequencing data of varying qualities, the performance of common genotyping and imputation methods was tested on different SNP panels. The results showed that parameters and methods developed for ancient DNA analysis performed better in degraded DNA analysis. Additionally, using a population reference panel representative of worldwide populations improved genotyping accuracy, but the low SNP density of commonly used forensic SNP panels could impact the reliability of the analysis. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84482295/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84819293" class="text-dark hover-underline">The (in)dependence of single-cell data inferences on model constructs</a> </h4> <p class="text-ellipsis-2">Catherine M. Grgicak, Klaas Slooten, Robert G. Cowell, Qhawe Bhembe, Desmond S. Lun</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Single-cell analysis is important in forensics, but different models may interpret the data differently. This study compared the weight of evidence on single-cell electropherograms among three models and found that they were mostly consistent, but there were some differences in some cases. The study also found that extreme stuttering was the main cause of the differences and proposed some interpretive adaptations to improve the situation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84819293/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84919066" class="text-dark hover-underline">Independent evaluation of an 11-CpG panel for age estimation in blood</a> </h4> <p class="text-ellipsis-2">Mie Rath Refn, Marie-Louise Kampmann, Agnes Vyoni, Jacob Tfelt-Hansen, Erik Sorensen, Sisse Rye Ostrowski, Mette Kongstad, Anastasia Aliferi, Federica Giangasparo, Niels Morling, David Ballard, Claus Borsting, Vania Pereira</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study evaluated the 11-CpG panel for age estimation and optimized it. The optimized panel was used to type Danish blood samples, and the results showed that DNA methylation at the 11 CpG loci was significantly correlated with age. A Danish age prediction model was constructed and compared with the original model, and the results were similar, but the original model had a bias towards underestimation, while the new model did not. The assay can reasonably accurately estimate the age of a single-source donor. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84919066/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="modal fade" id="export-citation" tabindex="-1"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal"><span>×</span></button> <h4 class="modal-title">Export Citation <b class="text-primary"></b></h4> </div> <div class="modal-body"> <div class="my-3 px-4 f16"> <form action="https://www.peeref.com/works/citation/download" method="GET" target="_blank"> <div class="radio"> <label> <input 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Comprehensive body fluid identification and contributor assignment by combining targeted sequencing of mRNA and coding region SNPs - Peeref