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(PDF) Detection of Influenza Virus Types A and B and Type A Subtypes (H1, H3, and H5) by Multiplex Polymerase Chain Reaction | Apiradee Theamboonlers - Academia.edu

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class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Detection of Influenza Virus Types A and B and Type A Subtypes (H1, H3, and H5) by Multiplex Polymerase Chain Reaction</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="33080744" href="https://independent.academia.edu/ApiradeeTheamboonlers"><img alt="Profile image of Apiradee Theamboonlers" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />Apiradee Theamboonlers</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2008, The Tohoku Journal of Experimental Medicine</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div 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RT-PCR products with unique sizes characteristic of each subtype were amplified by multiplex RT-PCRs, and sequence analysis of each amplicon was demonstrated to be specific for each subtype with 24 reference viruses. The specificity was demonstrated further with DNA or cDNA templates from 7 viruses, 5 bacteria, and 50 influenza A virus negative specimens. Furthermore, the assays could detect and subtype up to 105 dilution of each of the reference viruses that had an original infectivity titer of 106 EID50/ml. Of 188 virus isolates, the multiplex RT-PCR results agreed completely with individual RT-PCR subtyping results and with results obtained from virus isolations. Furthermore, the multiplex RT-PCR methods efficiently detected mixed infections with at least two different subtypes...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Development of multiplex rt-PCR assays for rapid detection and subtyping of influenza type A viruses from clinical specimens&quot;,&quot;attachmentId&quot;:91016025,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/86601677/Development_of_multiplex_rt_PCR_assays_for_rapid_detection_and_subtyping_of_influenza_type_A_viruses_from_clinical_specimens&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/86601677/Development_of_multiplex_rt_PCR_assays_for_rapid_detection_and_subtyping_of_influenza_type_A_viruses_from_clinical_specimens"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="43086916" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/43086916/Identification_of_genetic_markers_of_the_genetic_variability_of_avian_influenza_a_subtypes_h1n1_and_h7n9_Determination_of_primers_for_the_diagnostic_method_lamp_method_">Identification of genetic markers of the genetic variability of avian influenza a subtypes h1n1 and h7n9. Determination of primers for the diagnostic method (lamp method)</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2263402" href="https://kharkov-ua.academia.edu/BuriachenkoSemen">Buriachenko Semen</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Biopolym.cell , 2019</p><p class="ds-related-work--abstract ds2-5-body-sm">Background. HA, NA, and NP genes are potential candidates as markers of genetic variability in avian influenza virus in birds, animals, and humans. Aim. The purpose of this study was to extract the potential of the HA, NA, and NP genes as markers of the polymorphism of avian influenza A viruses in domestic animals and humans with respect to the subtype H7N9 birds. Methods. The study used the MEGA6 and VectorNTI-11 Smith-Waterman algorithm nucleotide sequences of the hemaglutinin (HA), neurominidase (NA) and nucleoprotein (NP) genes taken from the virus database. A common region at the 3’-end of the segments was used to select the reverse primer sequences for amplifying the HA-NA and NP genes. To select direct primers, the multiple alignment of all the HA, NA, and NP gene sequences deposited in the ISD database (www.flu.lanl.gov) was performed. Then, the consensus sequences were formed for the HA and NA-ta NP genes; the common conservative areas (motifs) and selected sequences of direct primers were found for the first and second stages of amplification in compliance with the general principles; the primers were chosen. The following algorithm was used to select oligonucleotide markers capable of specifically detecting of a specific subtype of AIV: all HA, NA and NP sequences in the database were divided into groups according to certain variants of hemagglutinin (H1 and H7), neuraminidase (N1 and N9) and nucleoprotein (NP). 40 resulting groups were divided into subgroups according to the origin of the virus (isolated from humans, birds, etc.), which formed a consensus sequence. Then, the most conservative motifs, limited by the primers of the second amplification stage, of the genomic segment, were determined. When it was impossible to identify a rather conservative motive, phylogenetic analysis of the sequences was performed within the subgroup with its division into smaller subgroups. Then a consensus sequence was formed and a search for conservative areas was conducted. Results. A total of 3500 sequences of hemagglutinin, neuraminidase, and AIV nucleoproein segments were analyzed. The variable structural regions of the nucleotide sequences of genes were determined. In all genes, single nucleotide substitutions are acquired, and the most polymorphic loci are located at the 3 ‘and 5’ ends of the sequences. The number of oligonucleotides selected to determine a specific variant of HA, NA and NP depended on the number of identified conservative regions in the amplified region and on the degree of variability within the region. Based on the analysis, the primers were selected (4 for each gene of two subtypes) for an isothermal loop amplification reaction of viral genes, the set was constructed consisting of 24 discriminating oligonucleotides for specific analysis of AIV subtypes. Conclusions. The results of the analysis showed that the identification of RNA mutations and the most variable gene loci could be used for the genotyping of influenza A viruses.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Identification of genetic markers of the genetic variability of avian influenza a subtypes h1n1 and h7n9. Determination of primers for the diagnostic method (lamp method)&quot;,&quot;attachmentId&quot;:63340336,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/43086916/Identification_of_genetic_markers_of_the_genetic_variability_of_avian_influenza_a_subtypes_h1n1_and_h7n9_Determination_of_primers_for_the_diagnostic_method_lamp_method_&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/43086916/Identification_of_genetic_markers_of_the_genetic_variability_of_avian_influenza_a_subtypes_h1n1_and_h7n9_Determination_of_primers_for_the_diagnostic_method_lamp_method_"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="49508544" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/49508544/A_Simple_Method_for_Molecular_Detection_of_Swine_Origin_and_Human_Origin_Influenza_A_Virus">A Simple Method for Molecular Detection of Swine-Origin and Human-Origin Influenza A Virus</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="140809118" href="https://independent.academia.edu/GouldErnest">Ernest Gould</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Vector-Borne and Zoonotic Diseases, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;A Simple Method for Molecular Detection of Swine-Origin and Human-Origin Influenza A Virus&quot;,&quot;attachmentId&quot;:67845022,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/49508544/A_Simple_Method_for_Molecular_Detection_of_Swine_Origin_and_Human_Origin_Influenza_A_Virus&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/49508544/A_Simple_Method_for_Molecular_Detection_of_Swine_Origin_and_Human_Origin_Influenza_A_Virus"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="86768858" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/86768858/Studies_on_Multiplex_RT_PCR_for_Detection_of_Avian_Influenza_Virus_Type_A_Group_and_Specific_H5_and_H7_Subtypes">Studies on Multiplex RT-PCR for Detection of Avian Influenza Virus Type A Group and Specific H5 and H7 Subtypes</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="198560018" href="https://independent.academia.edu/HuaguangLu">Huaguang Lu</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Veterinary Science &amp; Medicine, 2012</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Studies on Multiplex RT-PCR for Detection of Avian Influenza Virus Type A Group and Specific H5 and H7 Subtypes&quot;,&quot;attachmentId&quot;:91148152,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/86768858/Studies_on_Multiplex_RT_PCR_for_Detection_of_Avian_Influenza_Virus_Type_A_Group_and_Specific_H5_and_H7_Subtypes&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/86768858/Studies_on_Multiplex_RT_PCR_for_Detection_of_Avian_Influenza_Virus_Type_A_Group_and_Specific_H5_and_H7_Subtypes"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="15741979" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/15741979/Design_and_clinical_application_of_a_molecular_method_for_detection_and_typing_of_the_influenza_A_H1N1pdm_virus">Design and clinical application of a molecular method for detection and typing of the influenza A/H1N1pdm virus</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34918297" href="https://independent.academia.edu/ConcettaCastilletti">Concetta Castilletti</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Virological Methods, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Design and clinical application of a molecular method for detection and typing of the influenza A/H1N1pdm virus&quot;,&quot;attachmentId&quot;:42914550,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/15741979/Design_and_clinical_application_of_a_molecular_method_for_detection_and_typing_of_the_influenza_A_H1N1pdm_virus&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/15741979/Design_and_clinical_application_of_a_molecular_method_for_detection_and_typing_of_the_influenza_A_H1N1pdm_virus"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;continue-reading-button--sticky-ctas&quot;,&quot;attachmentId&quot;:44645579,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;download-pdf-button--sticky-ctas&quot;,&quot;attachmentId&quot;:44645579,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_44645579" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="91694383" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/91694383/Comparison_of_multiplex_RT_PCR_with_virus_isolation_for_detection_typing_and_sub_typing_of_influenza_virus_from_influenza_like_illness_cases">Comparison of multiplex RT-PCR with virus isolation for detection, typing and sub-typing of influenza virus from influenza-like illness cases</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="14596427" href="https://chouhants.academia.edu/PMali">Prof. 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class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2263402" href="https://kharkov-ua.academia.edu/BuriachenkoSemen">Buriachenko Semen</a></div><p class="ds-related-work--metadata ds2-5-body-xs">YOUNG SCIENTISTS CONFERENCE “YOUTH AND MODERN PROBLEMS OF MICROBIOLOGY AND VIROLOGY” (12-14 November 2019, Kyiv, Ukraine) , 2019</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;SELECTION OF PRIMERS FOR THE SUBUNITS OF HEMAGGLUTININ, NEURAMINIDASE AND NUCLEOPROTEIN SUBTYPES OF H1N1 AND H7N9 OF INFLUENZA 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data-collection-position="2" data-entity-id="24186227" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/24186227/Design_of_a_single_tube_RT_PCR_assay_for_the_diagnosis_of_human_infection_with_highly_pathogenic_influenza_A_H5_viruses">Design of a single tube RT-PCR assay for the diagnosis of human infection with highly pathogenic influenza A(H5) viruses</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="46492507" href="https://independent.academia.edu/PetricM">M. Petric</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Virological Methods, 2007</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Design of a single tube RT-PCR assay for the diagnosis of human infection with highly pathogenic influenza A(H5) viruses&quot;,&quot;attachmentId&quot;:44521910,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/24186227/Design_of_a_single_tube_RT_PCR_assay_for_the_diagnosis_of_human_infection_with_highly_pathogenic_influenza_A_H5_viruses&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/24186227/Design_of_a_single_tube_RT_PCR_assay_for_the_diagnosis_of_human_infection_with_highly_pathogenic_influenza_A_H5_viruses"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="3" data-entity-id="16572978" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/16572978/Comparison_of_three_non_nested_RT_PCR_for_the_detection_of_influenza_A_viruses">Comparison of three non-nested RT-PCR for the detection of influenza A viruses</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="35882908" href="https://independent.academia.edu/JeanmarieCohen">Jean-marie Cohen</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Clinical Virology, 2000</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Comparison of three non-nested RT-PCR for the detection of influenza A viruses&quot;,&quot;attachmentId&quot;:42433545,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/16572978/Comparison_of_three_non_nested_RT_PCR_for_the_detection_of_influenza_A_viruses&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/16572978/Comparison_of_three_non_nested_RT_PCR_for_the_detection_of_influenza_A_viruses"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="4" data-entity-id="22615508" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/22615508/Rapid_detection_and_simultaneous_subtype_differentiation_of_influenza_A_viruses_by_real_time_PCR">Rapid detection and simultaneous subtype differentiation of influenza A viruses by real time PCR</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="44074767" href="https://independent.academia.edu/BurrowsJulie">Julie Burrows</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Virological Methods, 2004</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Rapid detection and simultaneous subtype differentiation of influenza A viruses by real time PCR&quot;,&quot;attachmentId&quot;:43214109,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/22615508/Rapid_detection_and_simultaneous_subtype_differentiation_of_influenza_A_viruses_by_real_time_PCR&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" 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href="https://independent.academia.edu/MaZhiyong">Zhiyong Ma</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Chinese Science Bulletin, 2009</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR&quot;,&quot;attachmentId&quot;:47370159,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/27119052/Subtyping_of_type_A_influenza_by_sequencing_the_variable_regions_of_HA_gene_specifically_amplified_with_RT_PCR&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link 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data-author-id="35131397" href="https://independent.academia.edu/ViviSetiawaty">Vivi Setiawaty</a></div><p class="ds-related-work--metadata ds2-5-body-xs">BMC Research Notes, 2016</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus&quot;,&quot;attachmentId&quot;:80172740,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/70406072/Primer_development_to_obtain_complete_coding_sequence_of_HA_and_NA_genes_of_influenza_A_H3N2_virus&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link 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quantitative RT-PCR assay for rapid detection of Eurasian-lineage H 10 subtype influenza A virus</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="41293443" href="https://independent.academia.edu/LannyPace">Lanny Pace</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2016</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;L ETTER A quantitative RT-PCR assay for rapid detection of Eurasian-lineage H 10 subtype influenza A 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data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/6490278/Detection_of_H5_H7_and_H9_subtypes_of_avian_influenza_viruses_by_multiplex_reverse_transcription_polymerase_chain_reaction">Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="10328517" href="https://upm.academia.edu/KhatijahYusoff">Khatijah Yusoff</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Microbiological Research, 2009</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Detection of H5, H7 and H9 subtypes of avian influenza viruses 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Chambers</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Influenza and Other Respiratory Viruses, 2007</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Real-time RT-PCR assays for type and subtype detection of influenza A and B viruses&quot;,&quot;attachmentId&quot;:40311877,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/18888546/Real_time_RT_PCR_assays_for_type_and_subtype_detection_of_influenza_A_and_B_viruses&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" 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ds2-5-body-xs">ASIA LIFE SCIENCES .The Asian International Journal of Life Sciences, 2020</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Analysis of influenza virus A: Pathogenic strains H1N1\nand H7N9 as examples&quot;,&quot;attachmentId&quot;:65139660,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/44664258/Analysis_of_influenza_virus_A_Pathogenic_strains_H1N1_and_H7N9_as_examples&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" 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