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Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Frozen) (IHC (F)), Immunohistochemistry (Paraffin) (IHC (P)), Flow Cytometry (Flow), Immunoprecipitation (IP) and Immunomicroscopy (IM) applications. This antibody reacts with Hamster, Human, Mouse, Porcine, Rat samples. 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as a Method * define function as object methods * @type {Object} */ window.digitalData = { user: {}, page: {}, merchandising: {}, getQueryParam : function (param) { var index, key, value, hash; var hashes = window.location.href.slice(window.location.href.indexOf('?') + 1).split('&'); for (index = 0; index < hashes.length; index++) { hash = hashes[index].split('='); key = hash[0]; value = hash[1]; if (key.toUpperCase() === param.toUpperCase()) return value; } return ''; }, getValOnce: function (value, cookieName, expiry, timeUnit) { var currentDate = new Date; var currentCookieValue; // timeUnit of 'm' is minutes represented in milliseconds // thus 86400000 = 24 hours = 1 day var interval = timeUnit == 'm' ? 60000 : 86400000; cookieName = cookieName ? cookieName : 'digitaldata_gvo'; value = value ? value : ''; expiry = expiry ? expiry : 0; currentCookieValue = this.getCookie(cookieName); if (value) { currentDate.setTime(currentDate.getTime() + expiry * interval); this.setCookie(cookieName, value, expiry == 0 ? 0 : currentDate); } return value == currentCookieValue ? '' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); var refReg = document.referrer; var referrerDomain = refReg.match(/:\/\/([^/]+)\//); if (referrerDomain !== null && referrerDomain[1].indexOf(masterDomain) == -1) { this.merchandising.referrer = referrerDomain; } } document.addEventListener('registerMerchandisingData', function (event) { for (var key in event.detail) { console.log("Listener data copied", key, event.detail[key] ); digitalData.merchandising[key] = event.detail[key]; } // Map some analytics attributes to merchandising session attributes digitalData.merchandising.sessionId = digitalData.merchandising.analyticsVisitorID; digitalData.merchandising.s_vi_low = digitalData.merchandising.analyticsVisitorIDLow; digitalData.merchandising.s_vi_high = digitalData.merchandising.analyticsVisitorIDHigh; console.log("digitalData: Captured registerMerchandisingData event"); digitalData.merchandisingDataInitted = true; // Call emailCampaignRecord if the digitalDataAnalyticsListener object is loaded if (typeof digitalDataAnalyticsListener != "undefined") { console.log("digitalData: Calling email campaign record"); digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented. </p> </div> </div> </div> </div> <div class="pdp-components"> <div id="pdp-components" ng-controller="pdpMainController as $ctrl">  <div block-ui="main" class="block-ui-main"></div> <div class="hero hero-loading-height" ng-class="{'is-loaded':$ctrl.isLoaded, 'with-banner': 'false' == 'true'}"> <div class="container"> <div class="hero-content"> <div class="brand-breadcrumb to-hide-on-mobile row"> <div class="large-span-12 medium-span-12 target_page_breadcrumbs"> <div class="breadcrumb breadcrumb-hide-restricted-only"> <ul class="breadcrumb breadcrumb-hide-restricted-only hero-breadcrumbs"> <li class="active"> <a href="/us/en/home/life-science/antibodies/primary-antibodies.html" target="_self"> Primary Antibodies </a> <span class="divider">&rsaquo;</span> </li> <li class="active"> <a href="/antibody/primary/target/pdi" target="_self" class="targetLink"> PDI Antibodies</a> </li> </ul> </div> </div> </div> <svg xmlns="http://www.w3.org/2000/svg" display="none"> <symbol id="close-drawer-icon" viewBox="0 0 22.74 22.74"> <line x1="1.67" y1="1.67" x2="21.33" y2="21.33" style="fill:none;stroke:#555759;stroke-linecap:square;stroke-width:2px"/> <line x1="21.07" y1="1.41" x2="1.41" y2="21.07" style="fill:none;stroke:#555759;stroke-linecap:square;stroke-width:2px"/> </symbol> <symbol id="info-icon" viewBox="0 0 32 32"> <path fill="#1e8ae7" d="M16 10.688c-1.152 0-2.048-0.896-2.048-2.048s0.896-2.048 2.048-2.048c1.152 0 2.048 0.896 2.048 2.048 0 1.088-0.96 2.048-2.048 2.048zM17.792 22.976c0 0.768-0.064 1.152-0.256 1.536-0.256 0.576-0.832 0.896-1.6 0.896-0.704 0-1.28-0.32-1.6-0.896-0.192-0.384-0.256-0.768-0.256-1.536v-8.256c0-0.768 0.064-1.152 0.256-1.536 0.256-0.576 0.832-0.896 1.6-0.896 0.704 0 1.28 0.32 1.6 0.896 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class="details-hero" ng-click="$ctrl.toggleImages('ADVANCED_VERIFICATION')"> <a class="link adv-verification-link animated-scroll" href="/sg/en/home/life-science/antibodies/invitrogen-antibody-validation.html"> <svg class="svg-icon advanced-verification-icon"> <use href="#icon-advanced-verification"></use> </svg> Advanced Verification </a> <p class="adv-verification-detail" onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationSmLtr(event, this)"> This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. </p> </div> <div class="details-hero" onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationPublishedFigures(event, this)"> <a class="link published-link animated-scroll " href="#image-gallery" onclick="window.THERMO_FISHER.pdp.inp.publishedFiguresHero(event, this)"> <svg class="svg-icon published-figures-icon"> <use href="#icon-published-figures"></use> </svg> 58 Published Figures </a> </div> <div class="details-hero"> <a class="link references-link animated-scroll " href="#references-component-id" onclick="window.THERMO_FISHER.pdp.inp.referencesHero(event, this)"> <svg class="svg-icon references-icon"> <use href="#icon-references"></use> </svg> 87 References </a> </div> <div class="details-hero"> <a class="btn hero-btn hero-view-other" href="/antibody/primary/target/pdi"> View all (29) PDI antibodies </a> </div> </div> <div class="links-ab to-show-links-in-sm"> <div class="container"> <div class="link-container"> <a class="link-antibody" href="/order/genome-database/dataSheetPdf?producttype=antibody&productsubtype=antibody_primary&productId=MA3-019&version=Local" target="_blank" rel="nofollow"> <span> <svg class="svg-icon data-sheet-icon"> <use href="#icon-data-sheet"></use> </svg> </span> Datasheet </a> </div> <div class="link-container"> <a href="https://www.thermofisher.com/global/en/home/life-science/antibodies/antibodies-learning-center/antibody-protocols.html?icid=antibody-pdp-protocols" 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aria-labelledby="createCitationModal-title">  <div class="modal-content" role="document"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal">×</button> <h4 class="modal-title" id="createCitationModal-title">Cite PDI Monoclonal Antibody (RL90)</h4> </div> <div class="modal-body"> <textarea id="citationTextArea" rows="5">The following product was used in this experiment: PDI Monoclonal Antibody (RL90) from Thermo Fisher Scientific, catalog # MA3-019, RRID AB_2163120.</textarea> </div> <div class="modal-footer">  <button type="button" class="well-red btn btn-danger pull-left" data-dismiss="modal" onclick="copyToClipboard();" > Click to Copy </button>  </div> </div> </div> <script type="application/ld+json"> { "@context": "https://schema.org/", "@type": "BreadcrumbList", "itemListElement": [{ "@type": "ListItem", "position": 1, "name": "Home", "item":"https://www.thermofisher.com" },{ "@type": "ListItem", "position": 2, "name": "Antibodies", "item": "https://www.thermofisher.com/us/en/home/life-science/antibodies.html" },{ "@type": "ListItem", "position": 3, "name": "PDI", "item": "https://www.thermofisher.com/antibody/primary/target/pdi" }] } </script> </div> <div class="links-ab to-hide-links-in-sm"> <div class="container"> <div class="link-container"> <a class="link-antibody" href="/order/genome-database/dataSheetPdf?producttype=antibody&productsubtype=antibody_primary&productId=MA3-019&version=Local" target="_blank" rel="nofollow"> <span> <svg class="svg-icon data-sheet-icon"> <use href="#icon-data-sheet"></use> </svg> </span> Datasheet </a> </div> <div class="link-container"> <a href="https://www.thermofisher.com/global/en/home/life-science/antibodies/antibodies-learning-center/antibody-protocols.html?icid=antibody-pdp-protocols" target="_blank" class="link-antibody"> <span> <svg class="svg-icon protocols-icon"> <use href="#icon-protocols"></use> </svg> </span> Protocols </a> </div> <div class="link-container"> <a href="/us/en/home/technical-resources/contact-us.MA3019.html?supportType=TS" class="link-antibody"> <span> <svg class="svg-icon tech-support-icon"> <use href="#icon-tech-support"></use> </svg> </span> Tech Support </a> </div> </div> </div> <div id="image-gallery" class="scrollClass image-gallery-loading-height" ng-class="{'is-loaded':$ctrl.isLoaded}"> <div id="tabName" tab-name-value="changeTabValue"></div> <div id="originalProductLink" product-link-value="originalProductLinkValue"></div> <div class="gal-cntr container" tfs-media-gallery sku="'MA3-019'" media-items="{'Antibody Testing Data':[{'sku':'MA3-019','imageId':'27811','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_7.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_7.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI_Antibody_MA3-019_Immunofluorescence_7.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI_Antibody_MA3-019_Immunofluorescence_7.jpg?time\u003d20240507','sortOrder':'2','description':'Immunofluorescent analysis of Phalloidin (orange) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.','shortDescription':'Immunofluorescent analysis of Phalloidin (orange) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI_Antibody_MA3-019_Immunofluorescence_7.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'21938','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Colon-tissue.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Colon-tissue.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3-019_Immunohistochemistry_Colon-tissue.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3-019_Immunohistochemistry_Colon-tissue.jpg?time\u003d20240507','sortOrder':'3','description':'Immunohistochemistry was performed on normal deparaffinized Human colon tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PDI (Product # MA3-019) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.','shortDescription':'Immunohistochemistry was performed on normal deparaffinized Human colon tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))','title':'PDI Antibody (MA3-019) in IHC (P)','appAbv':'IHC (P)','appName':'Immunohistochemistry (Paraffin)','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3-019_Immunohistochemistry_Colon-tissue.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'31058','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3-019_WB_20130731115533.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3-019_WB_20130731115533.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3-019_WB_20130731115533.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3-019_WB_20130731115533.jpg?time\u003d20240507','sortOrder':'4','description':'Western blot analysis of PDI was performed by loading 25 µg of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 57 kDa.','shortDescription':'Western blot analysis of PDI was performed by loading 25 µg of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Sub... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3-019_WB_20130731115533.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'30236','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_3T3_20130708115155.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_3T3_20130708115155.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3-019_FACS_3T3_20130708115155.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3-019_FACS_3T3_20130708115155.jpg?time\u003d20240507','sortOrder':'5','description':'Flow cytometry analysis of PDI in NIH/3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.','shortDescription':'Flow cytometry analysis of PDI in NIH/3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) se... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Flow Cytometry (Flow)','title':'PDI Antibody (MA3-019) in Flow','appAbv':'Flow','appName':'Flow Cytometry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3-019_FACS_3T3_20130708115155.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'27810','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_6.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_6.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI_Antibody_MA3-019_Immunofluorescence_6.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI_Antibody_MA3-019_Immunofluorescence_6.jpg?time\u003d20240507','sortOrder':'6','description':'Immunofluorescent analysis of Phalloidin (green) and PDI (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.','shortDescription':'Immunofluorescent analysis of Phalloidin (green) and PDI (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:2... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI_Antibody_MA3-019_Immunofluorescence_6.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'27812','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_8.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_8.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI_Antibody_MA3-019_Immunofluorescence_8.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI_Antibody_MA3-019_Immunofluorescence_8.jpg?time\u003d20240507','sortOrder':'7','description':'Immunofluorescent analysis of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.','shortDescription':'Immunofluorescent analysis of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI_Antibody_MA3-019_Immunofluorescence_8.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'7663','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_NS-1.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_NS-1.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI_MA3-019_Immunofluorescence_NS-1.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI_MA3-019_Immunofluorescence_NS-1.jpg?time\u003d20240507','sortOrder':'8','description':'Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in NS-1 Cells.','shortDescription':'Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in NS-1 Cells.','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI_MA3-019_Immunofluorescence_NS-1.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'27809','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI_Antibody_MA3-019_Immunofluorescence_5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI_Antibody_MA3-019_Immunofluorescence_5.jpg?time\u003d20240507','sortOrder':'9','description':'Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/mL final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.','shortDescription':'Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI_Antibody_MA3-019_Immunofluorescence_5.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'84143','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI-Antibody-MA3019-IF-9.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI-Antibody-MA3019-IF-9.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI-Antibody-MA3019-IF-9.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI-Antibody-MA3019-IF-9.jpg?time\u003d20240507','sortOrder':'10','description':'Immunofluorescent analysis of PDI (red) in MDCK cells. The cells were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a PDI mouse monoclonal antibody (Product # MA3-019), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A27027) at a dilution of 1:1000 for 30 minutes at room temperature (red). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.','shortDescription':'Immunofluorescent analysis of PDI (red) in MDCK cells. The cells were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a PDI mouse monoclonal antibody (Product # MA3-019), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A27027) at a dilution of 1:1000 for 3... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI-Antibody-MA3019-IF-9.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'7662','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_HMVEC.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_HMVEC.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI_MA3-019_Immunofluorescence_HMVEC.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI_MA3-019_Immunofluorescence_HMVEC.jpg?time\u003d20240507','sortOrder':'11','description':'Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in HMVEC Cells.','shortDescription':'Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in HMVEC Cells.','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI_MA3-019_Immunofluorescence_HMVEC.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'7661','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_A549.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_A549.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI_MA3-019_Immunofluorescence_A549.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI_MA3-019_Immunofluorescence_A549.jpg?time\u003d20240507','sortOrder':'12','description':'Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in A549 Cells.','shortDescription':'Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in A549 Cells.','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI_MA3-019_Immunofluorescence_A549.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'7664','imageUrl':'https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_p19.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_p19.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/PDI_MA3-019_Immunofluorescence_p19.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/PDI_MA3-019_Immunofluorescence_p19.jpg?time\u003d20240507','sortOrder':'13','description':'Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in p19 Cells.','shortDescription':'Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in p19 Cells.','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'PDI_MA3-019_Immunofluorescence_p19.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'21939','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Lung-adenocarcinoma.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Lung-adenocarcinoma.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3-019_Immunohistochemistry_Lung-adenocarcinoma.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3-019_Immunohistochemistry_Lung-adenocarcinoma.jpg?time\u003d20240507','sortOrder':'14','description':'Immunohistochemistry was performed on cancer biopsies of deparaffinized Human lung adenocarcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PDI (Product # MA3-019) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.','shortDescription':'Immunohistochemistry was performed on cancer biopsies of deparaffinized Human lung adenocarcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))','title':'PDI Antibody (MA3-019) in IHC (P)','appAbv':'IHC (P)','appName':'Immunohistochemistry (Paraffin)','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3-019_Immunohistochemistry_Lung-adenocarcinoma.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'21940','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Tonsil-tissue.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Tonsil-tissue.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3-019_Immunohistochemistry_Tonsil-tissue.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3-019_Immunohistochemistry_Tonsil-tissue.jpg?time\u003d20240507','sortOrder':'15','description':'Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PDI (Product # MA3-019) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.','shortDescription':'Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibod... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))','title':'PDI Antibody (MA3-019) in IHC (P)','appAbv':'IHC (P)','appName':'Immunohistochemistry (Paraffin)','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3-019_Immunohistochemistry_Tonsil-tissue.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'617702','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3019-PDI-WB.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3019-PDI-WB.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3019-PDI-WB.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3019-PDI-WB.jpg?time\u003d20240507','sortOrder':'16','description':'Western blot was performed using Anti-PDI Mouse Monoclonal Antibody (Product # MA3-019) and a 57 kDa band corresponding to PDI was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2),NIH/3T3 (Lane 3), A-375 (Lane 4), Hep G2 (Lane 5), HL-60 (Lane 6), and PC-12 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).','shortDescription':'Western blot was performed using Anti-PDI Mouse Monoclonal Antibody (Product # MA3-019) and a 57 kDa band corresponding to PDI was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2),NIH/3T3 (Lane 3), A-375 (Lane 4), Hep G2 (Lane 5), HL-60 (Lane 6), and PC-12 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3019-PDI-WB.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'617703','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3019-PDI-siRNA-WB.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3019-PDI-siRNA-WB.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3019-PDI-siRNA-WB.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3019-PDI-siRNA-WB.jpg?time\u003d20240507','sortOrder':'17','description':'Knockdown of PDI was achieved by transfecting A-431 cells with PDI specific siRNAs (Silencer® select Product # s441). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the PDI knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-PDI Mouse Monoclonal Antibody (Product # MA3-019, 1:2000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Densitometric analysis of this Western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PDI.','shortDescription':'Knockdown of PDI was achieved by transfecting A-431 cells with PDI specific siRNAs (Silencer® select Product # s441). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the PDI knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-PDI Mouse Monoclonal Antibody (Product # MA3-019, 1:2000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3019-PDI-siRNA-WB.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'30237','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_K562_20130708115224.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_K562_20130708115224.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3-019_FACS_K562_20130708115224.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3-019_FACS_K562_20130708115224.jpg?time\u003d20240507','sortOrder':'18','description':'Flow cytometry analysis of PDI in K562 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.','shortDescription':'Flow cytometry analysis of PDI in K562 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) seconda... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Flow Cytometry (Flow)','title':'PDI Antibody (MA3-019) in Flow','appAbv':'Flow','appName':'Flow Cytometry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3-019_FACS_K562_20130708115224.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'30235','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_Hela_20130708115131.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_Hela_20130708115131.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3-019_FACS_Hela_20130708115131.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3-019_FACS_Hela_20130708115131.jpg?time\u003d20240507','sortOrder':'19','description':'Flow cytometry analysis of PDI in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.','shortDescription':'Flow cytometry analysis of PDI in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) seconda... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Flow Cytometry (Flow)','title':'PDI Antibody (MA3-019) in Flow','appAbv':'Flow','appName':'Flow Cytometry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA3-019_FACS_Hela_20130708115131.jpg','benchSciPubmedId':'0'}],'Published Figures':[{'sku':'MA3-019','imageId':'990813','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_21318_urn-x-wiley-15387933-media-jth15740-jth15740-fig-0003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_21318_urn-x-wiley-15387933-media-jth15740-jth15740-fig-0003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_21318_urn-x-wiley-15387933-media-jth15740-jth15740-fig-0003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_21318_urn-x-wiley-15387933-media-jth15740-jth15740-fig-0003.jpg?time\u003d20240507','sortOrder':'21','description':'3 FIGURE ER retention of VWF in ECFCs with VWF propeptide variants. A, Ex 4-5del; B, p.Cys633Arg; C, p.Arg273Trp; D, p.Pro293Glnfs*164 and p.Gln419* ECFCs. Co-staining of ECFCs for VWF (green) and the ER marker PDI (red) shows co-localization (yellow) indicating ER retention of the variant (diffuse) VWF in heterozygous and homozygous VWF propeptide ECFCs. In normal ECFCs (Unaffected III-5, III-6), VWF is in WPBs and not associated with the ER marker. Images were obtained using a Leica TCS SP8 microscope at 63x magnification, upgraded with Hyvolution software (scale bars \u003d 50 mum; and 10 mum for zoomed in panels). White boxes show the magnified area. The optical zoom is provided at the top right corner of each zoomed in image. C. Het, compound heterozygous; ECFCs, endothelial colony-forming cells; ER, endoplasmic reticulum; Het, heterozygous; Hom, homozygous; PDI, protein disulfide-isomerase; VWF, von Willebrand factor; WPBs, Weibel-Palade bodies','shortDescription':'3 FIGURE ER retention of VWF in ECFCs with VWF propeptide variants. A, Ex 4-5del; B, p.Cys633Arg; C, p.Arg273Trp; D, p.Pro293Glnfs*164 and p.Gln419* ECFCs. Co-staining of ECFCs for VWF (green) and the ER marker PDI (red) shows co-localization (yellow) indicating ER retention of the variant (diffuse) VWF in heterozygous and homozygous VWF propeptide ECFCs. In normal ECFCs (Unaffected III-5, III-6), VWF is in... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Jul 1, 2022, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of thrombosis and haemostasis : JTH','journalText':'Journal of thrombosis and haemostasis : JTH 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_21318_urn-x-wiley-15387933-media-jth15740-jth15740-fig-0003.jpg','benchSciPubmedId':'35466528'},{'sku':'MA3-019','imageId':'949509','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20021_spectrum.00422-21-f006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20021_spectrum.00422-21-f006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20021_spectrum.00422-21-f006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20021_spectrum.00422-21-f006.jpg?time\u003d20240507','sortOrder':'22','description':'FIG 6 The LTM-1 and LTM-2 motifs of NTPase have the potential to direct fusion proteins to the ER. (A) Schematic diagram of various NTPase(1-179) deletion mutants. The ability and degree of NTPase(1-179)-GFP and its deletion mutants for LD localization and for ER localization are summarized. (B) Colocalization of NTPase(1-179) deletion constructs with LDs or with the ER in cells. A7 cells were transfected with the indicated expression plasmids for 24 h, and then the transfected cells were dually stained with LipidTOX and anti-PDI antibody; scale bars, 10 mum. The quantitative results are plotted as mean +- SEM ( n \u003d 15 cells obtained from two independent experiments); *, P \u003c 0.05 for results compared to those from the empty vector group (GFP); #, P \u003c 0.05 for results compared to those from the NTPase(1-179)-GFP-transfected group. (C) Confocal microscopic analysis of the localization of NTPase(1-179)-GFP in cells at different time points (6, 18, and 24 h) after transfection. A7 cells were transfected with the NTPase(1-179)-GFP expression plasmid for 6, 18, or 24 h, and then the subcellular localization of this protein to LDs or the ER in transfected cells was analyzed by confocal microscopy; scale bars, 10 mum. The quantitative results are plotted as mean +- SEM ( n \u003d 15 cells obtained from two independent experiments); *, P \u003c 0.05 for results compared to those from the 6 h group.','shortDescription':'FIG 6 The LTM-1 and LTM-2 motifs of NTPase have the potential to direct fusion proteins to the ER. (A) Schematic diagram of various NTPase(1-179) deletion mutants. The ability and degree of NTPase(1-179)-GFP and its deletion mutants for LD localization and for ER localization are summarized. (B) Colocalization of NTPase(1-179) deletion constructs with LDs or with the ER in cells. A7 cells were transfected w... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Sep 3, 2021, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Microbiology spectrum','journalText':'Microbiology spectrum 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20021_spectrum.00422-21-f006.jpg','benchSciPubmedId':'34431704'},{'sku':'MA3-019','imageId':'949711','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20102_MGG3-9-e1777-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20102_MGG3-9-e1777-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20102_MGG3-9-e1777-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20102_MGG3-9-e1777-g002.jpg?time\u003d20240507','sortOrder':'23','description':'FIGURE 4 The intracellular localization of CatD and enzyme activity in No. 1002 cells. (a) The immunofluorescence cell localization of wild-type and mutant CatD expressed in No. 1002 cells. After transfecting vector, wild-type, and mutant plasmids into No. 1002 cells, the co-localization of the CatD protein (green) and lysosome (red), as well as the co-localization of the CatD protein (green) and endoplasmic reticulum (red) were observed. (b) The ratio of mature-CatD and pro-CatD in No. 1002 cells transfected with the wild-type and mutant of the CTSD gene, respectively. (c) The CatD activity of wild-type and mutant No. 1002 cells. CatD activity expressed as relative fluorescence units (RFU) per million cells','shortDescription':'FIGURE 4 The intracellular localization of CatD and enzyme activity in No. 1002 cells. (a) The immunofluorescence cell localization of wild-type and mutant CatD expressed in No. 1002 cells. After transfecting vector, wild-type, and mutant plasmids into No. 1002 cells, the co-localization of the CatD protein (green) and lysosome (red), as well as the co-localization of the CatD protein (green) and endoplasmi... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Oct 1, 2021, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Molecular genetics \u0026 genomic medicine','journalText':'Molecular genetics \u0026 genomic medicine 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20102_MGG3-9-e1777-g002.jpg','benchSciPubmedId':'34331747'},{'sku':'MA3-019','imageId':'949115','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17583_mbc-32-1594-g005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17583_mbc-32-1594-g005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17583_mbc-32-1594-g005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17583_mbc-32-1594-g005.jpg?time\u003d20240507','sortOrder':'24','description':'FIGURE 5: RUSH assay reveals mislocalization of B4GalT1 to endolysosomes in GARP-KO RPE1 cells. RPE1 cells were transfected with plasmids encoding Str-KDEL_flB4GalT1-SBP-mCherry in biotin-free medium. Following overnight incubation, biotin (40 uM) and cycloheximide (50 uM) were added for 0, 2, and 6 h. (A) Colocalization of B4GalT1 with ER marker PDI at time 0. (B, C) Colocalization of B4GalT1 with Golgi protein GM130 at 2 h (B) and 6 h (C). The graph on the right side of C shows the quantification of B4GalT1 and GM130 colocalization at 6 h of biotin/cycloheximide addition. Values in the bar graph represent the mean +- SD of the colocalization between B4GalT1 and GM130 in approximately 40 cells in WT and VPS54-KOs. Colocalization in VPS53-KO cells was measured using each field in approximately 20 cells. Statistical significance was calculated using one-way ANOVA. **** P \u003c\u003d 0.0001. (D) Colocalization of B4GalT1, GM130 and the endolysosomal marker CD63 after addition of biotin/cycloheximide mix for 6 h. Quantification of the colocalization between CD63 and B4GalT1 was done by Pearson\u0027s correlation coefficient analysis in approximately 25 cells. Statistical significance was calculated using one-way ANOVA. **** P \u003c\u003d 0.0001, * P \u003c\u003d 0.05.','shortDescription':'FIGURE 5: RUSH assay reveals mislocalization of B4GalT1 to endolysosomes in GARP-KO RPE1 cells. RPE1 cells were transfected with plasmids encoding Str-KDEL_flB4GalT1-SBP-mCherry in biotin-free medium. Following overnight incubation, biotin (40 uM) and cycloheximide (50 uM) were added for 0, 2, and 6 h. (A) Colocalization of B4GalT1 with ER marker PDI at time 0. (B, C) Colocalization of B4GalT1 with Golgi pr... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Aug 15, 2021, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Molecular biology of the cell','journalText':'Molecular biology of the cell 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17583_mbc-32-1594-g005.jpg','benchSciPubmedId':'34161137'},{'sku':'MA3-019','imageId':'838658','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12377_viruses-13-00216-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12377_viruses-13-00216-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12377_viruses-13-00216-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12377_viruses-13-00216-g003.jpg?time\u003d20240507','sortOrder':'25','description':'Figure 3 NS3 localizes to the ER when NS2B is provided in trans and in cis. ( A ) HEK293T cells were co-transfected with NS3-GFP and NS2B-V5 expressing plasmids ( a - c ). At 24 h post-transfection, cells were fixed and co-stained with anti-GFP and antibodies against PDI (red; a ), giantin (red; b ) or tubulin (red; c ). HEK293T cells were also triple-transfected with NS3-GFP, NS2B-V5/His and IKKepsilon-FLAG expressing plasmids ( d ) and triple stained with anti-GFP (green; d ), anti-V5/His (red; d ) and anti-IKKepsilon (blue; d ) antibodies. ( B ) HEK293T cells were transfected with NS2B-NS3-GFP ( a - c ) or co-transfected with NS2B-NS3-GFP and IKKepsilon-FLAG expressing plasmids ( d ) and processed for immunofluorescence at 24 h after transfection using antibodies against PDI (red; a ), giantin (red; b ), tubulin (red; c ) and IKKepsilon (red; d ). The detected viral and host proteins are listed on the top right corner of each panel. White arrows indicate colocalization between the GFP-tagged viral protein and the ER marker. Nuclear DNA was labeled with DAPI. Scale bar represents 10 mum. The main panels depict merged images with the side panels showing the individual channels. The transfection efficiency (TE), the co-transfection efficiency (Co-TE) and the triple-transfection efficiency (Triple-TE) for cells expressing one, two, or three gene constructs, respectively, are listed at the bottom of the panel. Images are representative of two independent transfection experiment','shortDescription':'Figure 3 NS3 localizes to the ER when NS2B is provided in trans and in cis. ( A ) HEK293T cells were co-transfected with NS3-GFP and NS2B-V5 expressing plasmids ( a - c ). At 24 h post-transfection, cells were fixed and co-stained with anti-GFP and antibodies against PDI (red; a ), giantin (red; b ) or tubulin (red; c ). HEK293T cells were also triple-transfected with NS3-GFP, NS2B-V5/His and IKKepsilon-FLA... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Jan 31, 2021, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Viruses','journalText':'Viruses 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12377_viruses-13-00216-g003.jpg','benchSciPubmedId':'33572517'},{'sku':'MA3-019','imageId':'838657','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12377_viruses-13-00216-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12377_viruses-13-00216-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12377_viruses-13-00216-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12377_viruses-13-00216-g002.jpg?time\u003d20240507','sortOrder':'26','description':'Figure 2 WNV NS3 is distributed throughout the cytoplasm, while NS2B remains in the ER in transfected cells. ( A ) NS3-GFP transfected ( a - c ) or NS3-GFP and IKKepsilon-FLAG co-transfected ( d ), HEK293T cells were fixed at 24 h post-transfection and immunostained with antibodies against protein disulfide isomerase (PDI), an ER marker, (red; a ), anti-GM130, a Golgi apparatus marker, (red; b ), anti-tubulin, a cytoskeletal marker, (red; c ), or anti-IKKepsilon, a soluble cytoplasmic protein marker, (red; d ). ( B ) NS2B-V5/His transfected ( a - c ) or NS2B-V5/His and IKKepsilon-FLAG co-transfected ( d ) HEK293T cells were fixed 24 h after transfection and immunolabeled with rabbit anti-PDI (green; a ), anti-giantin (green; b ), anti-tubulin (green; c ) or anti-IKKepsilon (green; d ) antibodies to visualize the ER, Golgi apparatus, cytoskeleton or the cytoplasmic protein kinase IKKepsilon protein, respectively. Visualized viral and host proteins are listed on the top right corner of each panel. Nuclear DNA was labeled with DAPI. Confocal microscopy images were of optical slice thickness ~1 mum. Scale bar, 10 mum. The main image depicts the merged image from three channels separately shown on the side. White arrows indicate colocalization between the green and red channels. The transfection efficiency (TE) for cells transfected with one construct or the co-transfection efficiency (Co-TE) for cells co-expressing the viral protein and IKKepsilon are listed at the bottom of each','shortDescription':'Figure 2 WNV NS3 is distributed throughout the cytoplasm, while NS2B remains in the ER in transfected cells. ( A ) NS3-GFP transfected ( a - c ) or NS3-GFP and IKKepsilon-FLAG co-transfected ( d ), HEK293T cells were fixed at 24 h post-transfection and immunostained with antibodies against protein disulfide isomerase (PDI), an ER marker, (red; a ), anti-GM130, a Golgi apparatus marker, (red; b ), anti-tubul... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Jan 31, 2021, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Viruses','journalText':'Viruses 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12377_viruses-13-00216-g002.jpg','benchSciPubmedId':'33572517'},{'sku':'MA3-019','imageId':'877466','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17116_fcell-08-00414-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17116_fcell-08-00414-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17116_fcell-08-00414-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17116_fcell-08-00414-g003.jpg?time\u003d20240507','sortOrder':'27','description':'FIGURE 3 Confocal analysis of the subcellular localization of FAM46A in transfected K562 cells. Cells were transfected with an expression construct encoding the FAM46A-EGFP protein, which was detected in the nuclear (A) and cytoplasmic (B) compartments. Within the nucleus (A) , FAM46A was predominantly confined in the chromatin domains more accessible to the transcription complexes (as indicated by the acK9-Histone H3 staining) and were mostly excluded from the regions with condensed chromatin structures (as indicated by the meK9-Histone H3 staining). In addition, significant co-localization of FAM46A with NuMA and lamin A/C was observed. DAPI staining defined the morphology of nuclei. Scale bar: 5 mum. Within the cytoplasm (B) , the majority of FAM46A was found to co-localize with the endoplasmic reticulum marker, protein disulfide-isomerase (PDI). By contrast, co-localization of FAM46A with trans-Golgi network marker gamma-adaptin (AP-1) and early endosome marker transferrin receptor (TrfR) is relatively low. DAPI staining defines the morphology of nuclei. Scale bar: 5 mum.','shortDescription':'FIGURE 3 Confocal analysis of the subcellular localization of FAM46A in transfected K562 cells. Cells were transfected with an expression construct encoding the FAM46A-EGFP protein, which was detected in the nuclear (A) and cytoplasmic (B) compartments. Within the nucleus (A) , FAM46A was predominantly confined in the chromatin domains more accessible to the transcription complexes (as indicated by the acK9... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Sep 28, 2020, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Frontiers in cell and developmental biology','journalText':'Frontiers in cell and developmental biology 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17116_fcell-08-00414-g003.jpg','benchSciPubmedId':'32528962'},{'sku':'MA3-019','imageId':'948395','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19457_gr6_lrg.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19457_gr6_lrg.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19457_gr6_lrg.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19457_gr6_lrg.jpg?time\u003d20240507','sortOrder':'28','description':'Fig. 6 Co-immunostaining of CypB and cell organelles in HCoV-229E-infected Huh7 cells . For the identification of the intense cyclophilin B bleb-like structures infected cells (MOI \u003d 1; 48 h p.i.) were co-stained with anti-CypB and antibodies directed against markers of the ER (anti-PDI), cis-GOLGI (anti-GM130), autophagosomes (anti-SQSTM1), anti-P-bodies (anti-hDcp1a) and stress granules (anti-PABP). Cyclophilin B normally distributes within the ER. In the presence of HCoV-229E, it intriguingly concentrates at bleb-like structures of the ER. Fig. 6','shortDescription':'Fig. 6 Co-immunostaining of CypB and cell organelles in HCoV-229E-infected Huh7 cells . For the identification of the intense cyclophilin B bleb-like structures infected cells (MOI \u003d 1; 48 h p.i.) were co-stained with anti-CypB and antibodies directed against markers of the ER (anti-PDI), cis-GOLGI (anti-GM130), autophagosomes (anti-SQSTM1), anti-P-bodies (anti-hDcp1a) and stress granules (anti-PABP). Cyclo... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Jan 1, 2020, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Antiviral research','journalText':'Antiviral research 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19457_gr6_lrg.jpg','benchSciPubmedId':'31634494'},{'sku':'MA3-019','imageId':'739395','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_7658_41467_2019_9549_Fig6_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_7658_41467_2019_9549_Fig6_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_7658_41467_2019_9549_Fig6_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_7658_41467_2019_9549_Fig6_HTML.jpg?time\u003d20240507','sortOrder':'29','description':'Fig. 6 Disruption of ER-Golgi trafficking disassembles vRNP hotspots. A549 cells were infected or mock-infected with PR8 virus at an MOI of 3. a At 90 min p.i., 2 mug mL -1 of brefeldin A (BFA) or 10 mug mL -1 nocodazole (NOC) were added and incubated for 10 h until the supernatant was collected and viral titres determined or cells were harvested in Laemmli\u0027s and NP levels detected by western blotting. Statistical analysis of data was performed using a non-parametric one-way ANOVA, followed by Friedman\u0027s multiple comparisons test (* p \u003c 0.05). b - d At 8 hpi, cells were also treated or mock-treated with 2 mug mL -1 of BFA for 1 h. b Cells were immunostained for the ER marker PDI (in green), the viral protein NP (in red) and the cis -Golgi marker GM130 (in gray) and imaged by confocal microscopy. Selected areas of the cytoplasm are marked by white boxes and displayed on the top left corner of the images. Bar \u003d 10 mum. c The frequency distribution of NP inclusions within the three size categories (in mum 2 ), the number of inclusions per mum 2 , and the percentage of NP staining that is inside inclusions were plotted for each condition. Statistical analysis of data was performed using a non-parametric Kruskal-Wallis test, followed by Dunn\u0027s multiple comparisons test (*** p \u003c 0.001). An average of 60 cells from 2 independent experiments was analyzed per condition. d Infected cells were stained for the host protein Rab11 (in green) and the viral proteins NP (in re','shortDescription':'Fig. 6 Disruption of ER-Golgi trafficking disassembles vRNP hotspots. A549 cells were infected or mock-infected with PR8 virus at an MOI of 3. a At 90 min p.i., 2 mug mL -1 of brefeldin A (BFA) or 10 mug mL -1 nocodazole (NOC) were added and incubated for 10 h until the supernatant was collected and viral titres determined or cells were harvested in Laemmli\u0027s and NP levels detected by western blotting. ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Apr 9, 2019, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_7658_41467_2019_9549_Fig6_HTML.jpg','benchSciPubmedId':'30967547'},{'sku':'MA3-019','imageId':'739396','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_7658_41467_2019_9549_Fig7_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_7658_41467_2019_9549_Fig7_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_7658_41467_2019_9549_Fig7_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_7658_41467_2019_9549_Fig7_HTML.jpg?time\u003d20240507','sortOrder':'30','description':'Fig. 7 Disruption of ER exit sites dissolves viral inclusions. HeLa cells were transfected with plasmids encoding GFP, SAR1 WT-GFP (SAR1) or SAR1-GTP-GFP (SAR1-GTP) and, 24 h later, infected or mock-infected with PR8 virus, at an MOI of 10. At 16 hpi, cells were fixed and processed for immunofluorescence. a Cells were stained for the viral protein NP (in red) and for the ER protein PDI (in gray). Areas highlighted by the white box are shown on the right of each panel. b The number of inclusions per mum 2 , the percentage of NP staining that is inside inclusions and the frequency distribution of NP inclusions within the three area categories (in mum 2 ) were plotted for each condition. Statistical analysis of data was performed using a non-parametric Kruskal-Wallis test, followed by Dunn\u0027s multiple comparisons test (*** p \u003c 0.001). More than 40 cells from 2 independent experiments were analyzed per condition. c Infected cells were stained for the viral protein HA and imaged by confocal microscopy. Bar \u003d 10 mum','shortDescription':'Fig. 7 Disruption of ER exit sites dissolves viral inclusions. HeLa cells were transfected with plasmids encoding GFP, SAR1 WT-GFP (SAR1) or SAR1-GTP-GFP (SAR1-GTP) and, 24 h later, infected or mock-infected with PR8 virus, at an MOI of 10. At 16 hpi, cells were fixed and processed for immunofluorescence. a Cells were stained for the viral protein NP (in red) and for the ER protein PDI (in gray). Areas hi... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Apr 9, 2019, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_7658_41467_2019_9549_Fig7_HTML.jpg','benchSciPubmedId':'30967547'},{'sku':'MA3-019','imageId':'836679','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_10952_F6.small.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_10952_F6.small.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_10952_F6.small.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_10952_F6.small.jpg?time\u003d20240507','sortOrder':'31','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Sep 28, 2018, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_10952_F6.small.jpg','benchSciPubmedId':'30139741'},{'sku':'MA3-019','imageId':'723070','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6358_zbc0421894430006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6358_zbc0421894430006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6358_zbc0421894430006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6358_zbc0421894430006.jpg?time\u003d20240507','sortOrder':'32','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Sep 28, 2018, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6358_zbc0421894430006.jpg','benchSciPubmedId':'30139741'},{'sku':'MA3-019','imageId':'524835','imageUrl':'https://www.thermofisher.com/antibody/images/650/41598_2018_23483_Fig4_HTML-1-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/41598_2018_23483_Fig4_HTML-1-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/41598_2018_23483_Fig4_HTML-1-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/41598_2018_23483_Fig4_HTML-1-20190221105219.jpg?time\u003d20240507','sortOrder':'33','description':'Figure 4 Search for potential mechanisms involved in Brucella abortus -induced mitochondrial fragmentation ( a ). TOM20 immunostaining in HeLa cells pre-incubated for 30 min with the indicated concentration of ruthenium red and infected with B. abortus 2308 mCherry with or without the inhibitor - 48 h PI (Representative of n \u003d 3). Green: TOM20 (Alexa488)/Red: B. abortus 2308 (mCherry)/Turquoise: Nuclei (Hoechst) ( b , c ). Aspect ratio ( b ) and end point/branch point ratio ( c ) of HeLa cells pre-incubated for 30 min with the indicated concentration of ruthenium red and infected (white) or not (black) with B. abortus 2308 mCherry with or without the inhibitor - 48 h PI. Results represent means +- SD for three independent experiments (n \u003d 3). Statistical analysis: two-way ANOVA on Box Cox transformed data. P value for interaction: 0.446 and 0.051 respectively. The numbers indicated in the columns represent the number of cells analysed for each condition. ( d ) PDI and TOM20 co-immunostaining in HeLa cells infected or not (control) with B. abortus 2308 mCherry - 48 h PI (Representative of n \u003d 3). Green: PDI (Alexa488)/Blue: TOM20 (Alexa633)/Red: B. abortus 2308 (mCherry)/Turquoise: Nuclei (Hoechst). ( e ) Western blot analysis of P-IRE1 and IRE1 abundance in RAW 264.7 macrophages infected or not with B. abortus 2308 mCherry - 48 h PI (Representative of n \u003d 3 in different conditions). RAW 264.7 cells treated for 6 h with 10 uM tunicamycin and then left for 18 h for','shortDescription':'Figure 4 Search for potential mechanisms involved in Brucella abortus -induced mitochondrial fragmentation ( a ). TOM20 immunostaining in HeLa cells pre-incubated for 30 min with the indicated concentration of ruthenium red and infected with B. abortus 2308 mCherry with or without the inhibitor - 48 h PI (Representative of n \u003d 3). Green: TOM20 (Alexa488)/Red: B. abortus 2308 (mCherry)/Turquoise: Nuclei... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Mar 26, 2018, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Scientific reports','journalText':'Scientific reports 2018 - ','sourceType':'3','benchsci':true,'imageName':'41598_2018_23483_Fig4_HTML-1-20190221105219.jpg','benchSciPubmedId':'29581535'},{'sku':'MA3-019','imageId':'336454','imageUrl':'https://www.thermofisher.com/antibody/images/650/41598_2017_16947_Fig8_HTML-20180918151446.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/41598_2017_16947_Fig8_HTML-20180918151446.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/41598_2017_16947_Fig8_HTML-20180918151446.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/41598_2017_16947_Fig8_HTML-20180918151446.jpg?time\u003d20240507','sortOrder':'34','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Dec 8, 2017, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Scientific reports','journalText':'Scientific reports 2017 - ','sourceType':'3','benchsci':true,'imageName':'41598_2017_16947_Fig8_HTML-20180918151446.jpg','benchSciPubmedId':'29222525'},{'sku':'MA3-019','imageId':'624617','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_4170_zjv0041833180003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_4170_zjv0041833180003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_4170_zjv0041833180003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_4170_zjv0041833180003.jpg?time\u003d20240507','sortOrder':'35','description':'FIG 3 N-terminal 179-aa region of GII-NTPase is required for vesicle formation and ER colocalization. (A) Schematic diagram of the full-length and deletion constructs of F-NTPase. The abilities of F-NTPase and its deletions to promote vesicle formation and ER colocalization are summarized in the diagram. (B) Western blot analysis of F-NTPase and its deletions expressed in 293T cells. (C) Representative confocal immunofluorescence images of F-NTPase constructs (green) and PDI (red) in A7 cells. White arrows indicate the positions of vesicle-like structures formed by F-NTPase or its deletions. Yellow arrows represent the colocalization between the indicated F-NTPase constructs and PDI in the merged images. Scale bars, 10 mum.','shortDescription':'FIG 3 N-terminal 179-aa region of GII-NTPase is required for vesicle formation and ER colocalization. (A) Schematic diagram of the full-length and deletion constructs of F-NTPase. The abilities of F-NTPase and its deletions to promote vesicle formation and ER colocalization are summarized in the diagram. (B) Western blot analysis of F-NTPase and its deletions expressed in 293T cells. (C) Representative co... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Mar 1, 2018, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of virology','journalText':'Journal of virology 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_4170_zjv0041833180003.jpg','benchSciPubmedId':'29212938'},{'sku':'MA3-019','imageId':'313077','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3019-28489076-PDI-ncomms15244-f4.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3019-28489076-PDI-ncomms15244-f4.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3019-28489076-PDI-ncomms15244-f4.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3019-28489076-PDI-ncomms15244-f4.jpg?time\u003d20240507','sortOrder':'36','description':'Figure 4 Subcellular localization of DP4 and DP5 in the presence or absence of Ii. ( a - c ) HEK293 cells were transfected with the indicated combinations of genes. Fixed cells were permeabilized and stained for DP (red) in conjunction with an ER marker, PDI (green, left panels), an early endosomal marker, EEA1 (green, middle panels) or a late endosomal/lysosomal marker, LAMP-1 (green, right panels) and analysed by confocal microscopy. Inset boxes indicate the areas depicted at higher magnification. Scale bar in all images, 10 mum. Quantification of co-localized spots represents means+-s.d. of three counted cells in each condition. ns, not significant; * P \u003c0.05, ** P \u003c0.01, *** P \u003c0.001 by unpaired, two-tailed Welch\u0026apos;s t -test.','shortDescription':'Figure 4 Subcellular localization of DP4 and DP5 in the presence or absence of Ii. ( a - c ) HEK293 cells were transfected with the indicated combinations of genes. Fixed cells were permeabilized and stained for DP (red) in conjunction with an ER marker, PDI (green, left panels), an early endosomal marker, EEA1 (green, middle panels) or a late endosomal/lysosomal marker, LAMP-1 (green, right panels) and ana... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'May 10, 2017, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2017 - ','sourceType':'3','benchsci':true,'imageName':'MA3019-28489076-PDI-ncomms15244-f4.jpg','benchSciPubmedId':'28489076'},{'sku':'MA3-019','imageId':'836842','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11105_F5.small.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11105_F5.small.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11105_F5.small.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11105_F5.small.jpg?time\u003d20240507','sortOrder':'37','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Aug 1, 2015, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of lipid research','journalText':'Journal of lipid research 2015 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11105_F5.small.jpg','benchSciPubmedId':'26089538'},{'sku':'MA3-019','imageId':'364748','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3019-26089538-PDI-1583fig5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3019-26089538-PDI-1583fig5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3019-26089538-PDI-1583fig5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3019-26089538-PDI-1583fig5.jpg?time\u003d20240507','sortOrder':'38','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Aug 1, 2015, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of lipid research','journalText':'Journal of lipid research 2015 - ','sourceType':'3','benchsci':true,'imageName':'MA3019-26089538-PDI-1583fig5.jpg','benchSciPubmedId':'26089538'},{'sku':'MA3-019','imageId':'836280','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_10632_F9.small.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_10632_F9.small.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_10632_F9.small.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_10632_F9.small.jpg?time\u003d20240507','sortOrder':'39','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Mar 15, 2013, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2013 - 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(Lane 1) HEK293 cells; (lane 2) HEK293/GFP; (lane 3) HEK293/mDC-STAMP-GFP; and (lane 4) HEK293/mDC-STAMP-GFP plus tunicamycin. Western blots were stained with anti-GFP mAb. The arrow indicates unglycosylated mDC-STAMP-GFP. (B) HEK293 cells, stably transfected with mDC-STAMP-GFP or hDC-STAMP-GFP constructs, respectively, were stained with antibodies against beta1 integrins, against the protein present in the ER (anti-PDI), and the intermediate compartment (anti-ERGIC-53). Cells adherent to the fibronectin-coated slides were visualized by CLSM for GFP autofluorescence (green) and the markers (blue) as indicated. (C) Immunoelectron microscopy of HEK293 cells stably transfected with hDC-STAMP-GFP. Sections were stained with the antibody against GFP and incubated with the 10-nm gold beads. The arrows point to the gold beads at the nuclear membrane (left) and in the ER (right). The other gold particles in the left panel are located on ER structures, as is more clearly shown in the right panel. No staining was visible at the mitochondria (M) or in the nucleus (N). Original magnification, 20,000x.','shortDescription':'3 (A) Western blot analysis of HEK293 cells transfected with mDC-STAMP-GFP constructs. (Lane 1) HEK293 cells; (lane 2) HEK293/GFP; (lane 3) HEK293/mDC-STAMP-GFP; and (lane 4) HEK293/mDC-STAMP-GFP plus tunicamycin. Western blots were stained with anti-GFP mAb. The arrow indicates unglycosylated mDC-STAMP-GFP. (B) HEK293 cells, stably transfected with mDC-STAMP-GFP or hDC-STAMP-GFP constructs, respectively, w... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Mar 1, 2005, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of leukocyte biology','journalText':'Journal of leukocyte biology 2005 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11097_jlb0337-fig-0003.jpg','benchSciPubmedId':'15601667'},{'sku':'MA3-019','imageId':'836837','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11097_jlb0337-fig-0004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11097_jlb0337-fig-0004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11097_jlb0337-fig-0004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11097_jlb0337-fig-0004.jpg?time\u003d20240507','sortOrder':'57','description':'4 DC-STAMP-GFP localizes to the ER in DC. Mo-IDC transduced with the adenovirus Ad5fib35 encoding hDC-STAMP-GFP (green) were stained with a beta2-integrin antibody (red), an ER marker (anti-PDI, blue), and an intermediate compartment marker (anti-ERGIC-53, blue), and fluorescence was visualized by CLSM.','shortDescription':'4 DC-STAMP-GFP localizes to the ER in DC. Mo-IDC transduced with the adenovirus Ad5fib35 encoding hDC-STAMP-GFP (green) were stained with a beta2-integrin antibody (red), an ER marker (anti-PDI, blue), and an intermediate compartment marker (anti-ERGIC-53, blue), and fluorescence was visualized by CLSM.','altTag':'PDI Antibody in Immunocytochemistry (ICC/IF)','title':'PDI Antibody (MA3-019) in ICC/IF','appAbv':'ICC/IF','dateTime':'Mar 1, 2005, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of leukocyte biology','journalText':'Journal of leukocyte biology 2005 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11097_jlb0337-fig-0004.jpg','benchSciPubmedId':'15601667'},{'sku':'MA3-019','imageId':'953735','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20766_JEM_20201916_Fig1.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20766_JEM_20201916_Fig1.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20766_JEM_20201916_Fig1.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20766_JEM_20201916_Fig1.jpg?time\u003d20240507','sortOrder':'58','description':'Figure 1. EN1 expression is up-regulated in fibrotic skin. (A) Representative immunofluorescence stainings for EN1 (green) and costainings with P4H (red) and CD45 (magenta) in the dermis of healthy donors and SSc patients at 1,000-fold magnification (scale bars \u003d 20 um). Hematoxylin and eosin stainings (200-fold magnification, scale bars \u003d 100 um), Voronoi diagrams, and percentages of EN1-positive fibroblasts from all fibroblasts (defined as P4H + CD45 - cells) are included. Data are shown from one experiment ( n \u003e\u003d 4). (B and C) EN1 mRNA (B) and EN1 intracellular or secreted protein levels (C) in cultured fibroblasts or their supernatants from healthy donors and SSc patients. Representative Western blot images and quantifications are included (C). Data are shown from one experiment ( n \u003d 3). (D and E) En1 protein levels in the skin of mice challenged with bleomycin (D) or Tsk-1 mice (E) and their respective controls. Representative Western blot images and quantifications are included. Data are compiled from two independent experiments ( n \u003e\u003d 4). (F and G) Representative immunofluorescence stainings for EN1 (green) and costainings with Vimentin (red) and CD45 (magenta) in the dermis of mice challenged with bleomycin and their controls at 1,000-fold magnification (F; scale bars \u003d 20 um) and in the hypodermis of Tsk-1 mice and their controls, respectively, at 1,000-fold magnification (G; scale bars \u003d 20 um). Hematoxylin and eosin stainings (100-fold magnification, scale bars \u003d','shortDescription':'Figure 1. EN1 expression is up-regulated in fibrotic skin. (A) Representative immunofluorescence stainings for EN1 (green) and costainings with P4H (red) and CD45 (magenta) in the dermis of healthy donors and SSc patients at 1,000-fold magnification (scale bars \u003d 20 um). Hematoxylin and eosin stainings (200-fold magnification, scale bars \u003d 100 um), Voronoi diagrams, and percentages of EN1-positive fibroblas... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))','title':'PDI Antibody (MA3-019) in IHC (P)','appAbv':'IHC (P)','dateTime':'Sep 6, 2021, 4:00:00 AM','appName':'Immunohistochemistry (Paraffin)','type':'Published Figures','journalTitle':'The Journal of experimental medicine','journalText':'The Journal of experimental medicine 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20766_JEM_20201916_Fig1.jpg','benchSciPubmedId':'34259830'},{'sku':'MA3-019','imageId':'953982','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20766_JEM_20201916_FigS1.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20766_JEM_20201916_FigS1.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20766_JEM_20201916_FigS1.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20766_JEM_20201916_FigS1.jpg?time\u003d20240507','sortOrder':'59','description':'Figure S1. EN1 expression in healthy and SSc skin. (A-C) Representative immunofluorescence stainings for EN1 (green) and costainings with CD31 (A) and CD45 (B; both magenta) in the dermis of healthy donors and SSc patients at 1,000-fold magnification (scale bars \u003d 20 um). Hematoxylin and eosin stainings (200-fold magnification, scale bars \u003d 100 um) are included. Data for A-C were obtained from n \u003e\u003d 4 biological samples from two independent experiments. (D) Composition of clusters from the segmented IMC images in ventral and dorsal skin biopsies from SSc patients. (E) tSNE plot showing distribution of cells from ventral and dorsal SSc skin across clusters. (F) tSNE plot showing expression of EN1 across the ventral and dorsal SSc clusters. Data for D-F were obtained from n \u003d 8 biological samples from four SSc patients (paired ventral and dorsal biopsies).','shortDescription':'Figure S1. EN1 expression in healthy and SSc skin. (A-C) Representative immunofluorescence stainings for EN1 (green) and costainings with CD31 (A) and CD45 (B; both magenta) in the dermis of healthy donors and SSc patients at 1,000-fold magnification (scale bars \u003d 20 um). Hematoxylin and eosin stainings (200-fold magnification, scale bars \u003d 100 um) are included. Data for A-C were obtained from n \u003e\u003d 4 biolog... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunohistochemistry (IHC)','title':'PDI Antibody (MA3-019) in IHC','appAbv':'IHC','dateTime':'Sep 6, 2021, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'The Journal of experimental medicine','journalText':'The Journal of experimental medicine 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20766_JEM_20201916_FigS1.jpg','benchSciPubmedId':'34259830'},{'sku':'MA3-019','imageId':'739392','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_8268_fncel-12-00450-g009.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_8268_fncel-12-00450-g009.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_8268_fncel-12-00450-g009.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_8268_fncel-12-00450-g009.jpg?time\u003d20240507','sortOrder':'60','description':'FIGURE 9 Immunohistochemical localization of m2R in neuronal compartments involved in endocytosis, synthesis, maturation, and degradation in fixed hippocampal neurons. Hippocampal neurons were transfected with m2R-WT. Neurons were stimulated with CCh at 30 muM for 6, 20 min and 1 h fixed, and processed for visualization of m2R together with markers of intraneuronal compartments and observed by confocal microscopy. ( A-C) 6 min after CCh stimulation (30 muM), some m2R immunopositive punta colocalize with CHC in clathrin-coated pits, EEA1 in early endosomes and Rab9 in late endosomes (arrow heads). (D-E) Twenty minutes after CCh stimulation (30 muM), we failed to detect no colocalization of m2R with PDI, a marker of endoplasmic reticulum and GM130, a marker of Golgi apparatus. (F-F) One hour after CCh stimulation (30 muM), some m2R immunopositive puncta colocalize with CathD, a marker of lysosomes (arrow heads). The quantitative analysis of the colocalization of m2R and markers of subcellular compartment in neurons was performed using the Jacop Plugin of ImageJ and statistical data are reported from the Costes\u0027s randomization-based colocalization module (see Materials and Methods). Data are expressed as a Pearson\u0027s coefficient (pc) and pc were compared using the Mann-Whitney U -test. Our analysis shows that the colocalization of the immunofluorescent signals for m2R with CHC, EEA1, Rab9, and CathD is higher after treatment with CCh compared to untreated neurons (CHC, Rab9','shortDescription':'FIGURE 9 Immunohistochemical localization of m2R in neuronal compartments involved in endocytosis, synthesis, maturation, and degradation in fixed hippocampal neurons. Hippocampal neurons were transfected with m2R-WT. Neurons were stimulated with CCh at 30 muM for 6, 20 min and 1 h fixed, and processed for visualization of m2R together with markers of intraneuronal compartments and observed by confocal micr... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Immunohistochemistry (IHC)','title':'PDI Antibody (MA3-019) in IHC','appAbv':'IHC','dateTime':'Sep 29, 2020, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Frontiers in cellular neuroscience','journalText':'Frontiers in cellular neuroscience 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_8268_fncel-12-00450-g009.jpg','benchSciPubmedId':'30555302'},{'sku':'MA3-019','imageId':'364746','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3019-20638985-PDI-nihms-215207-f0003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3019-20638985-PDI-nihms-215207-f0003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3019-20638985-PDI-nihms-215207-f0003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3019-20638985-PDI-nihms-215207-f0003.jpg?time\u003d20240507','sortOrder':'61','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunohistochemistry','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunohistochemistry','altTag':'PDI Antibody in Immunohistochemistry (IHC)','title':'PDI Antibody (MA3-019) in IHC','appAbv':'IHC','dateTime':'Aug 30, 2010, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Molecular and cellular endocrinology','journalText':'Molecular and cellular endocrinology 2010 - ','sourceType':'3','benchsci':true,'imageName':'MA3019-20638985-PDI-nihms-215207-f0003.jpg','benchSciPubmedId':'20638985'},{'sku':'MA3-019','imageId':'879348','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17264_pone.0242960.g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17264_pone.0242960.g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17264_pone.0242960.g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17264_pone.0242960.g003.jpg?time\u003d20240507','sortOrder':'62','description':'10.1371/journal.pone.0242960.g003 Fig 3 Endoplasmic reticulum chaperones are downregulated in CTEPH-EC. (A) Representative blot of the GRP94 expression in static, low, and high magnitude shear stress (0, 5, and 15 dynes/cm 2 ). Data are presented as the mean +- standard error: (*) HPAEC vs CTEPH-EC, (\u0026) HPAEC 15 vs CTEPH-EC 15. (n \u003d 5-7). (B) GRP78 and PDI expression before and after shear stress exposure as described in methods. Data are presented as the mean +- standard error: (*) HPAEC vs HPAEC 15, (#) CTEPH-EC vs CTEPH-EC 5 (\u0026) HPAEC 15 vs CTEPH-EC 15, P \u003c 0.05. (n \u003d 6-7). All of the samples were normalized to ss-actin and run in the same gel.','shortDescription':'10.1371/journal.pone.0242960.g003 Fig 3 Endoplasmic reticulum chaperones are downregulated in CTEPH-EC. (A) Representative blot of the GRP94 expression in static, low, and high magnitude shear stress (0, 5, and 15 dynes/cm 2 ). Data are presented as the mean +- standard error: (*) HPAEC vs CTEPH-EC, (\u0026) HPAEC 15 vs CTEPH-EC 15. (n \u003d 5-7). (B) GRP78 and PDI expression before and after shear stress exposure a... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Jan 18, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17264_pone.0242960.g003.jpg','benchSciPubmedId':'33270690'},{'sku':'MA3-019','imageId':'876146','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig2_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig2_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_7652_41419_2019_1402_Fig2_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_7652_41419_2019_1402_Fig2_HTML.jpg?time\u003d20240507','sortOrder':'63','description':'Fig. 2 Nox NADPH oxidase expression and superoxide generation by HCT116 cells with overactivated KRas. a Nox1, NoxA1, p67phox, NoxO1, and p47phox basal protein expression by western analysis normalized for GADPH. Nox1 immunoblot densities were quantified using Odyssey software. * P \u003c 0.05, ANOVA plus Tukey\u0027s multiple comparisons test ( n \u003d 3). b RhoGDIalpha, RhoA, and Rac1 basal protein expression by western analysis normalized for GAPDH. Rac1 immunoblot densities were quantified using Odyssey software. ** P \u003c 0.01; *** P \u003c 0.005, ANOVA plus Tukey\u0027s multiple comparisons test ( n \u003d 3). c Nox1 complex scheme. d Rac1 basal activity, using Rac1 G-LISA activation assay (Cytoskeleton,inc). Test t * p \u003c 0.05, ( n \u003d 2). e Rac1 protein expression by western analysis after 72 h of PDIA1 silencing. Rac1 Immunoblot densities were quantified using Odyssey software ** P \u003c 0.01; ns: not significant, Student\u0027s t -test ( n \u003d 3). f Western analysis of NoxO1 and RhoGDIalpha protein expression before and after PDIA1 silencing, normalized for GAPDH. RhoGDIalpha immunoblot densities were quantified using Odyssey software, **** P \u003c 0.001; ns: not significant, Student\u0027s t -test ( n \u003d 3). g Superoxide production after 72h of PDIA1 silencing in HCT116 cells treated or not with 10 uM of NoxA1ds Nox1\u0027s peptide inhibitor. EOH 2-hidroxyethidiium relative superoxide production, si-PDI, si-RNA against PDIA1 protein; Scrmb NoxA1ds, NoxA1ds negative control peptide; NoxA1ds, Nox1 peptide inhibitor. * P \u003c 0.05','shortDescription':'Fig. 2 Nox NADPH oxidase expression and superoxide generation by HCT116 cells with overactivated KRas. a Nox1, NoxA1, p67phox, NoxO1, and p47phox basal protein expression by western analysis normalized for GADPH. Nox1 immunoblot densities were quantified using Odyssey software. * P \u003c 0.05, ANOVA plus Tukey\u0027s multiple comparisons test ( n \u003d 3). b RhoGDIalpha, RhoA, and Rac1 basal protein expression by wester... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Feb 13, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death \u0026 disease','journalText':'Cell death \u0026 disease 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_7652_41419_2019_1402_Fig2_HTML.jpg','benchSciPubmedId':'30760703'},{'sku':'MA3-019','imageId':'739394','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig5_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig5_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_7652_41419_2019_1402_Fig5_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_7652_41419_2019_1402_Fig5_HTML.jpg?time\u003d20240507','sortOrder':'64','description':'Fig. 5 Effects of PDIA1 silencing in signaling targets and EMT marker expression. PathScan array analysis. All array\u0027s spots were quantified and analyzed using ImageJ software. PDIA1 silencing was checked by immunoblot analysis. Ctrl, si-RNA negative control; si-PDI, si-RNA against PDIA1; ERK1/2, Thr202/Tyr204 phosphorylation; Stat1, Tyr701 phosphorylation; Stat3, Tyr705 phosphorylation; Akt, Thr308 phosphorylation; Akt, Ser473 phosphorylation; AMPKa, Thr172 phosphorylation, s6 ribosomal protein, Ser235/236 phosphorylation; mTOR, Ser2448 phosphorylation; HSP27, Ser78 phosphorylation; Bad, Ser112 phosphorylation; p70 S6 kinase, Thr389 phosphorylation; PRAS40, Thr246 phosphorylation; p53, Ser15 phosphorylation; p38, Thr180/Tyr182 phosphorylation; SAPK/JNK, Thr183/Tyr185 phosphorylation; PARP, Asp214 cleavage; Caspase-3, Asp175 Cleavage; GSK-3beta, Ser9 phosphorylation. b GSK-3beta, Ser9 phosphorylation plot. c Stat3, Tyr705 phosphorylation. d Basal E-cadherin (E-cad) protein expression by immunoblot normalized for GAPDH ( n \u003d 3). e Immunoblot for E-cadherin before and after PDIA1 silencing in Caco2 cells ( n \u003d 2). f Immunoblot for E-cadherin before and after PDIA1 silencing in HKE3 cells.; ns: not significant, Student\u0027s t test ( n \u003d 3). g Immunoblot for E-cadherin before and after PDIA1 silencing in HCT116 cells * P \u003c 0.05, Student\u0027s t test ( n \u003d 3). Immunoblot densities were quantified using Odyssey software','shortDescription':'Fig. 5 Effects of PDIA1 silencing in signaling targets and EMT marker expression. PathScan array analysis. All array\u0027s spots were quantified and analyzed using ImageJ software. PDIA1 silencing was checked by immunoblot analysis. Ctrl, si-RNA negative control; si-PDI, si-RNA against PDIA1; ERK1/2, Thr202/Tyr204 phosphorylation; Stat1, Tyr701 phosphorylation; Stat3, Tyr705 phosphorylation; Akt, Thr308 phospho... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Feb 13, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death \u0026 disease','journalText':'Cell death \u0026 disease 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_7652_41419_2019_1402_Fig5_HTML.jpg','benchSciPubmedId':'30760703'},{'sku':'MA3-019','imageId':'739393','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time\u003d20240507','sortOrder':'65','description':'Fig. 1 Role of PDIA1 in oxidant generation by colon carcinoma cells with distinct levels of KRas activation. a KRas expression and activity in Caco2, HKE3, and HCT116 cells. Active KRas was pulled down with GST-RBD beads from lysates of serum-starved cells treated with EGF 25 ng/mL for 10 min. Aliquots of the lysates were blotted for total KRas and GAPDH as loading control. b RNAseq analysis of P4HB (PDIA1) gene in Caco2, HCT116, and HCT15 cells, showing mean expression in FPKM (Fragments Per Kilobase Million), P4HB fold-change expression in HCT15 vs. Caco2 (q \u003c 0.000001) and HCT116 vs. Caco2 (q \u003c 0.000001). c PDIA1 basal protein expression by western analysis, normalized for GAPDH ( n \u003d 3) and quantified using Odyssey software. d Intracellular PDIA1 titration, using Human P4HB ELISA Pair Set (SinoBiological). Test t ** p \u003c 0.01, ( n \u003d 4); e-g ROS production after 72 h of PDIA1 silencing, measured by HPLC analysis of DHE oxidation products. DHE oxidation produces, among many others, two major products: 2-hidroxyethidium (EOH), which is representative of superoxide species and ethidium, representative of other oxidant species. Ctrl negative, si-RNA control; si-PDI, si-RNA against PDIA1 protein. Representative immunoblots of PDI silencing for each cell type Test t * p \u003c 0.05; ** p \u003c 0.01. For Caco2 cells, n \u003d 3; HKE3 cells, n \u003d 4; HCT116 cells, n \u003d 5','shortDescription':'Fig. 1 Role of PDIA1 in oxidant generation by colon carcinoma cells with distinct levels of KRas activation. a KRas expression and activity in Caco2, HKE3, and HCT116 cells. Active KRas was pulled down with GST-RBD beads from lysates of serum-starved cells treated with EGF 25 ng/mL for 10 min. Aliquots of the lysates were blotted for total KRas and GAPDH as loading control. b RNAseq analysis of P4HB (PDI... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Feb 13, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death \u0026 disease','journalText':'Cell death \u0026 disease 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_7652_41419_2019_1402_Fig1_HTML.jpg','benchSciPubmedId':'30760703'},{'sku':'MA3-019','imageId':'731052','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6819_12192_2018_964_Fig3_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6819_12192_2018_964_Fig3_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6819_12192_2018_964_Fig3_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6819_12192_2018_964_Fig3_HTML.jpg?time\u003d20240507','sortOrder':'66','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Jan 1, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell stress \u0026 chaperones','journalText':'Cell stress \u0026 chaperones 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6819_12192_2018_964_Fig3_HTML.jpg','benchSciPubmedId':'30645756'},{'sku':'MA3-019','imageId':'731051','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6819_12192_2018_964_Fig2_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6819_12192_2018_964_Fig2_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6819_12192_2018_964_Fig2_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6819_12192_2018_964_Fig2_HTML.jpg?time\u003d20240507','sortOrder':'67','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Jan 1, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell stress \u0026 chaperones','journalText':'Cell stress \u0026 chaperones 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6819_12192_2018_964_Fig2_HTML.jpg','benchSciPubmedId':'30645756'},{'sku':'MA3-019','imageId':'731289','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6496_41418_2018_197_Fig6_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6496_41418_2018_197_Fig6_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6496_41418_2018_197_Fig6_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6496_41418_2018_197_Fig6_HTML.jpg?time\u003d20240507','sortOrder':'68','description':'Fig. 6 Caveolin-1 expression precludes DRP1 phosphorylation and redistribution during the early phase of ER stress. a Mock and CAV1 cells in control condition (con) or under early ER stress with tunicamycin (tun) were fractionated and processed for detection of phospho-DRP1 and DRP1 by western blotting using PDI as a loading control. C cytosol, LM light membranes (microsomes), HM heavy membranes (crude mitochondria). b Quantification of DRP1 phosphorylation analysed in ( a ) ( n \u003d 3). c Quantification of membrane-bound DRP1 distribution analysed in a ( n \u003d 3). d Experimental groups as in ( a ) were processed for immunofluorescence analysis. CAV1, mtHSP70 (mitochondrial marker, Mito) and DRP1 were stained with respective antibodies and then imaged using confocal microscopy. Merge between DRP1 and CAV1 images shows colocalized areas in yellow. Merge between DRP1 and Mito images shows colocalized areas in cyan. In merge images of Mito, DRP1 and CAV1, triple colocalization areas appear as white pixels. e CAV1 fluorescence per cell was quantified from images obtained in d ( n \u003d 3). f DRP1-CAV1 colocalization was quantified as Manders\u0027 coefficients of images obtained in ( d ) ( n \u003d 3). g DRP1-Mito colocalization was quantified as Manders\u0027 coefficients of images obtained in ( d ) ( n \u003d 3). h From images obtained in d , thresholded CAV1 (C) and Mito (M) images were generated. The intersection between M and DRP1 corresponds to mitochondrial DRP1 (DRP1 mito ). Merge between M and C ima','shortDescription':'Fig. 6 Caveolin-1 expression precludes DRP1 phosphorylation and redistribution during the early phase of ER stress. a Mock and CAV1 cells in control condition (con) or under early ER stress with tunicamycin (tun) were fractionated and processed for detection of phospho-DRP1 and DRP1 by western blotting using PDI as a loading control. C cytosol, LM light membranes (microsomes), HM heavy membranes (crude mito... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Jul 1, 2019, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death and differentiation','journalText':'Cell death and differentiation 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6496_41418_2018_197_Fig6_HTML.jpg','benchSciPubmedId':'30209302'},{'sku':'MA3-019','imageId':'723646','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6496_41418_2018_197_Fig3_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6496_41418_2018_197_Fig3_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6496_41418_2018_197_Fig3_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6496_41418_2018_197_Fig3_HTML.jpg?time\u003d20240507','sortOrder':'69','description':'Fig. 3 Caveolin-1 accumulates in mitochondria-associated ER membranes, precluding their remodelling upon early-phase ER stress. a Wild-type HeLa cells were labelled by immunogold staining with antibodies against CAV1. Blue arrowheads indicate CAV1 signals at the plasma membrane; red arrowheads indicate CAV1 signals at ER cisternae; yellow arrowheads indicate CAV1 at the ER-mitochondria interface. In the lower right panel, the ER is highlighted in red and mitochondria (mito) in green. Scale bar: 1 um. b Cells lysates were fractionated by differential sedimentation. HM heavy membranes (crude mitochondria), LM light membranes (microsomes). c HeLa mock and CAV1 cells in control condition (con) or under early ER stress with tunicamycin (tun) were processed and fractionated as in b . CAV1 levels were analysed by western blotting using PDI as a loading control. d Quantification of membrane-bound CAV1 analysed in c ( n \u003d 3). e HM fraction obtained as in ( b ) was separated into MAM and pure mitochondria (mito) fractions by Percoll density gradient centrifugation. f Markers of cell fractionation procedures shown in b and e : FACL4 short isoform (mito), long isoform (MAM); COX IV (mito), Calnexin (CNX) (LM and MAM), SERCA2b (LM) and PDI (C, LM and HM). g Experimental groups as in c were processed and fractionated as in e . CAV1 levels were analysed by western blotting using PDI as a loading control. h Quantification of CAV1 in MAM analysed in g ( n \u003d 3). i Experimental groups as in c w','shortDescription':'Fig. 3 Caveolin-1 accumulates in mitochondria-associated ER membranes, precluding their remodelling upon early-phase ER stress. a Wild-type HeLa cells were labelled by immunogold staining with antibodies against CAV1. Blue arrowheads indicate CAV1 signals at the plasma membrane; red arrowheads indicate CAV1 signals at ER cisternae; yellow arrowheads indicate CAV1 at the ER-mitochondria interface. In the low... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Jul 1, 2019, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death and differentiation','journalText':'Cell death and differentiation 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6496_41418_2018_197_Fig3_HTML.jpg','benchSciPubmedId':'30209302'},{'sku':'MA3-019','imageId':'730756','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6701_nihms679942f1.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6701_nihms679942f1.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6701_nihms679942f1.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6701_nihms679942f1.jpg?time\u003d20240507','sortOrder':'70','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Apr 7, 2015, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Science signaling','journalText':'Science signaling 2015 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6701_nihms679942f1.jpg','benchSciPubmedId':'25852189'},{'sku':'MA3-019','imageId':'333899','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3019-24406246-PDI-gr1(20365).jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3019-24406246-PDI-gr1(20365).jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3019-24406246-PDI-gr1(20365).jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3019-24406246-PDI-gr1(20365).jpg?time\u003d20240507','sortOrder':'71','description':'Fig. 1 Western blot analysis of Cyp3a2, Sec61alpha, Oatp1a1, Ntcp, Mrp2, and PDI in SER and RER isolated from Wistar and TR - rat livers. 5 mug (Cyp3a2, Mrp2), 50 mug (Sec61alpha) or 100 mug (Oatp1a1, Ntcp) protein was loaded per lane and separated by SDS-PAGE as given in section 2.4. Samples were probed against Cyp3a2, Sec61alpha, Oatp1a1, Ntcp, Mrp2, and PDI as described in 2.4. PDI was used as loading control in SER and RER fractions. Basolateral membrane fraction (BLM) isolated from Sprague Dawley rat liver was used as positive control for Oatp1a1 and Ntcp expression. Canalicular (CM) membrane fraction isolated from Sprague Dawley rat liver was used as positive control for Mrp2 expression. The figure shows a representative Western blot of at least two independent SER and RER isolations.','shortDescription':'Fig. 1 Western blot analysis of Cyp3a2, Sec61alpha, Oatp1a1, Ntcp, Mrp2, and PDI in SER and RER isolated from Wistar and TR - rat livers. 5 mug (Cyp3a2, Mrp2), 50 mug (Sec61alpha) or 100 mug (Oatp1a1, Ntcp) protein was loaded per lane and separated by SDS-PAGE as given in section 2.4. Samples were probed against Cyp3a2, Sec61alpha, Oatp1a1, Ntcp, Mrp2, and PDI as described in 2.4. PDI was used as loading co... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Mar 1, 2014, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Biochemical pharmacology','journalText':'Biochemical pharmacology 2014 - ','sourceType':'3','benchsci':true,'imageName':'MA3019-24406246-PDI-gr1(20365).jpg','benchSciPubmedId':'24406246'},{'sku':'MA3-019','imageId':'730743','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6594_zbc0141342890002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6594_zbc0141342890002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6594_zbc0141342890002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6594_zbc0141342890002.jpg?time\u003d20240507','sortOrder':'75','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Mar 15, 2013, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2013 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6594_zbc0141342890002.jpg','benchSciPubmedId':'23344956'},{'sku':'MA3-019','imageId':'620654','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_1760_nihms-327775-f0003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_1760_nihms-327775-f0003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_1760_nihms-327775-f0003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_1760_nihms-327775-f0003.jpg?time\u003d20240507','sortOrder':'79','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Dec 29, 2011, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Neuroscience','journalText':'Neuroscience 2011 - ','sourceType':'3','benchsci':true,'imageName':'tfs_1760_nihms-327775-f0003.jpg','benchSciPubmedId':'21978884'},{'sku':'MA3-019','imageId':'490228','imageUrl':'https://www.thermofisher.com/antibody/images/650/jlb0989-fig-0004-1-20190103084817.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/jlb0989-fig-0004-1-20190103084817.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/jlb0989-fig-0004-1-20190103084817.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/jlb0989-fig-0004-1-20190103084817.jpg?time\u003d20240507','sortOrder':'80','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Oct 1, 2009, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Journal of leukocyte biology','journalText':'Journal of leukocyte biology 2009 - ','sourceType':'3','benchsci':true,'imageName':'jlb0989-fig-0004-1-20190103084817.jpg','benchSciPubmedId':'19564574'},{'sku':'MA3-019','imageId':'836638','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_10913_F6.small.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_10913_F6.small.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_10913_F6.small.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_10913_F6.small.jpg?time\u003d20240507','sortOrder':'81','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Jun 20, 2008, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2008 - ','sourceType':'3','benchsci':true,'imageName':'tfs_10913_F6.small.jpg','benchSciPubmedId':'18426796'},{'sku':'MA3-019','imageId':'349954','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3019-18426796-PDI-zbc0280840940006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3019-18426796-PDI-zbc0280840940006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3019-18426796-PDI-zbc0280840940006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3019-18426796-PDI-zbc0280840940006.jpg?time\u003d20240507','sortOrder':'82','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot','altTag':'PDI Antibody in Western Blot (WB)','title':'PDI Antibody (MA3-019) in WB','appAbv':'WB','dateTime':'Jun 20, 2008, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2008 - ','sourceType':'3','benchsci':true,'imageName':'MA3019-18426796-PDI-zbc0280840940006.jpg','benchSciPubmedId':'18426796'},{'sku':'MA3-019','imageId':'490227','imageUrl':'https://www.thermofisher.com/antibody/images/650/jlb0989-fig-0002-1-20190103084817.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/jlb0989-fig-0002-1-20190103084817.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/jlb0989-fig-0002-1-20190103084817.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/jlb0989-fig-0002-1-20190103084817.jpg?time\u003d20240507','sortOrder':'83','description':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Neutralization','shortDescription':'Published figure using PDI monoclonal antibody (Product # MA3-019) in Neutralization','altTag':'PDI Antibody in Neutralization (Neu)','title':'PDI Antibody (MA3-019) in Neu','appAbv':'Neu','dateTime':'Oct 1, 2009, 4:00:00 AM','appName':'Neutralization','type':'Published Figures','journalTitle':'Journal of leukocyte biology','journalText':'Journal of leukocyte biology 2009 - ','sourceType':'3','benchsci':true,'imageName':'jlb0989-fig-0002-1-20190103084817.jpg','benchSciPubmedId':'19564574'},{'sku':'MA3-019','imageId':'880144','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_15962_FEBS_6339_f3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_15962_FEBS_6339_f3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_15962_FEBS_6339_f3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_15962_FEBS_6339_f3.jpg?time\u003d20240507','sortOrder':'84','description':'3 Increased binding of vitronectin and LM609 to Mn 2+ -stimulated endothelial cells requires free thiols in alpha V beta 3 and active PDI. Endothelial cells were stimulated with Mn 2+ , and their binding of LM609 (A, B) and vitronectin (C, D) was evaluated by flow cytometry in the absence or presence of the membrane-impermeant sulfhydryl group reagent PCMBS, a PDI-specific inhibitor (A, C), bacitracin (B), or blocking antibody to PDI (mAb 019) (D).','shortDescription':'3 Increased binding of vitronectin and LM609 to Mn 2+ -stimulated endothelial cells requires free thiols in alpha V beta 3 and active PDI. Endothelial cells were stimulated with Mn 2+ , and their binding of LM609 (A, B) and vitronectin (C, D) was evaluated by flow cytometry in the absence or presence of the membrane-impermeant sulfhydryl group reagent PCMBS, a PDI-specific inhibitor (A, C), bacitracin (B), ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody in Neutralization (Neu)','title':'PDI Antibody (MA3-019) in Neu','appAbv':'Neu','dateTime':'Apr 1, 2008, 4:00:00 AM','appName':'Neutralization','type':'Published Figures','journalTitle':'The FEBS journal','journalText':'The FEBS journal 2008 - ','sourceType':'3','benchsci':true,'imageName':'tfs_15962_FEBS_6339_f3.jpg','benchSciPubmedId':'18331351'}],'Advanced Verification':[{'sku':'MA3-019','imageId':'617701','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA3019-PDI-siRNA-AV-WB.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA3019-PDI-siRNA-AV-WB.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA3019-PDI-siRNA-AV-WB.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA3019-PDI-siRNA-AV-WB.jpg?time\u003d20240507','sortOrder':'1','description':'Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with PDI siRNA and reduction of signal was observed in Western Blot using PDI Monoclonal Antibody (Product # MA3-019). {KD}','shortDescription':'Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with PDI siRNA and reduction of signal was observed in Western Blot using PDI Monoclonal Antibody (Product # MA3-019). {KD}','altTag':'PDI Antibody','title':'PDI Antibody (MA3-019)','appAbv':'KD','appName':'Knockdown','type':'Advanced Verification','journalText':'','sourceType':'2','benchsci':false,'imageName':'MA3019-PDI-siRNA-AV-WB.jpg','benchSciPubmedId':'0'},{'sku':'MA3-019','imageId':'757950','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time\u003d20240507','sortOrder':'20','description':'Fig. 1 Role of PDIA1 in oxidant generation by colon carcinoma cells with distinct levels of KRas activation. a KRas expression and activity in Caco2, HKE3, and HCT116 cells. Active KRas was pulled down with GST-RBD beads from lysates of serum-starved cells treated with EGF 25 ng/mL for 10 min. Aliquots of the lysates were blotted for total KRas and GAPDH as loading control. b RNAseq analysis of P4HB (PDIA1) gene in Caco2, HCT116, and HCT15 cells, showing mean expression in FPKM (Fragments Per Kilobase Million), P4HB fold-change expression in HCT15 vs. Caco2 (q\u003c0.000001) and HCT116 vs. Caco2 (q\u003c0.000001). c PDIA1 basal protein expression by western analysis, normalized for GAPDH (n\u003d3) and quantified using Odyssey software. d Intracellular PDIA1 titration, using Human P4HB ELISA Pair Set (SinoBiological). Test t **p\u003c0.01, (n\u003d4); e-g ROS production after 72 h of PDIA1 silencing, measured by HPLC analysis of DHE oxidation products. DHE oxidation produces, among many others, two major products: 2-hidroxyethidium (EOH), which is representative of superoxide species and ethidium, representative of other oxidant species. Ctrl negative, si-RNA control; si-PDI, si-RNA against PDIA1 protein. Representative immunoblots of PDI silencing for each cell type Test t *p\u003c0.05; **p\u003c0.01. For Caco2 cells, n\u003d3; HKE3 cells, n\u003d4; HCT116 cells, n\u003d5 {KD}','shortDescription':'Fig. 1 Role of PDIA1 in oxidant generation by colon carcinoma cells with distinct levels of KRas activation. a KRas expression and activity in Caco2, HKE3, and HCT116 cells. Active KRas was pulled down with GST-RBD beads from lysates of serum-starved cells treated with EGF 25 ng/mL for 10 min. Aliquots of the lysates were blotted for total KRas and GAPDH as loading control. b RNAseq analysis of P4HB (PDIA1)... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'PDI Antibody','title':'PDI Antibody (MA3-019)','appAbv':'KD','appName':'Knockdown','type':'Advanced Verification','journalText':'','sourceType':'7','benchsci':true,'imageName':'tfs_7652_41419_2019_1402_Fig1_HTML.jpg','benchSciPubmedId':'0'}]}" image-displayed-id="'27811'" product-sub-type="'antibody_primary'" prod-type="'antibody'" page-type="PDP" product-id="'MA3-019'"> <div class="tabs"> <ul class="nav nav-pills"> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Antibody Testing Data"> <a class="tab-text"> Antibody Testing Data (18) </a> </li> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Published Figures"> <a class="tab-text"> Published Figures (58) </a> </li> <li class="tab" 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<span class="current-img"> 1 </span> / <span class="img-total-count"> 78</span></p> </div> <div class = "verification-certificate hide" > <span class="icon-certification"></span> <span class="adv-cert-text">{{ $ctrl.currentElement.advancedVerification.fullName }}</span> <div class="cert-provider">{{ $ctrl.currentElement.advancedVerification.text }}</div> </div> <h2 class="product-name" ng-show="!$ctrl.isVideo"> PDI Antibody (MA3-019) in ICC/IF </h2> <div ng-show="!$ctrl.isVideo"> <div class="image-desc"> <div class="product-desc-text viewing-more-long-description"> <span class="image-journal-text" > </span> <span class="short-desc"> Immunofluorescent analysis of Phalloidin (orange) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution ... <a href="#" class="images-view-more-text" >View More</a> </span> <span class="verification-info hide" ng-if="$ctrl.hasVerificationMethod"> <a rel="nofollow" ng-href="{{$ctrl.currentElement.advancedVerification.uri}}" >{{ $ctrl.currentElement.advancedVerification.fullName }} validation info.</a> </span> <a href="#" class="images-view-more-text hide" ng-if="$ctrl.showViewMore" onclick="window.THERMO_FISHER.inp.openImageGalleryModal(event, this)"> View more </a> </div> </div> <div class="img-desc-footer"> <div class="benchsci-pubmed-footer"> <div ng-cloak ng-if="$ctrl.currentElement.benchsci || $ctrl.sourceType === '3'" class="images-benchsci-footer"> <div class="images-benchsci-link" tabindex="0"> <span>Published figure supplied by</span> <span class="icon-benchsci-logo"> <svg id="Layer_1" data-name="Layer 1" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 89.75 23.21"><defs><style>.cls-1{fill:#222222;}</style></defs><title>benchsci-logo</title><g id="logo-blue-copy-3"><g id="logo-blue-copy-2"><path id="path14" class="cls-1" d="M34.54,11.49a2.45,2.45,0,0,0,1.59-2.34c0-1.69-1.21-2.94-3.32-2.94H28.88v10.9h4.21a3.06,3.06,0,0,0,3.37-3A2.57,2.57,0,0,0,34.54,11.49ZM31,8h1.49c1,0,1.57.53,1.57,1.36a1.36,1.36,0,0,1-1.55,1.37H31Zm1.72,7.35H31V12.47h1.75a1.42,1.42,0,0,1,1.62,1.46C34.34,14.79,33.72,15.34,32.69,15.34Z"/><path id="path16" class="cls-1" d="M41.34,9.32a3.8,3.8,0,0,0-3.72,4,3.83,3.83,0,0,0,3.91,4.05A3.39,3.39,0,0,0,45,15l-1.71-.51a1.67,1.67,0,0,1-1.72,1.15,1.85,1.85,0,0,1-1.91-1.73H45s0-.34,0-.63C45.09,10.81,43.69,9.32,41.34,9.32Zm-1.66,3.15A1.63,1.63,0,0,1,41.37,11a1.52,1.52,0,0,1,1.69,1.49Z"/><path id="path18" class="cls-1" d="M50.58,9.35a2.48,2.48,0,0,0-2.18,1.14v-1h-2v7.57h2.05V12.75a1.42,1.42,0,0,1,1.41-1.57,1.34,1.34,0,0,1,1.4,1.52v4.42h2V12.35C53.32,10.68,52.46,9.35,50.58,9.35Z"/><path id="path20" class="cls-1" d="M58.2,15.47a1.94,1.94,0,0,1-1.94-2.14,1.92,1.92,0,0,1,1.91-2.12,1.64,1.64,0,0,1,1.67,1.27l1.83-.61a3.45,3.45,0,0,0-3.55-2.55,3.83,3.83,0,0,0-2.82,1.16,3.88,3.88,0,0,0-1.1,2.85,3.9,3.9,0,0,0,4,4,3.5,3.5,0,0,0,3.53-2.55l-1.8-.6A1.72,1.72,0,0,1,58.2,15.47Z"/><path id="path22" class="cls-1" d="M67,9.35a2.72,2.72,0,0,0-2.07.83V6h-2V17.12h2V12.67a1.43,1.43,0,0,1,1.42-1.48,1.34,1.34,0,0,1,1.4,1.53v4.41h2V12.35A2.67,2.67,0,0,0,67,9.35Z"/><path id="path24" class="cls-1" d="M75.82,11.05l-1.63-.35a1.58,1.58,0,0,1-1.4-1.59A1.93,1.93,0,0,1,74.9,7.3a2.21,2.21,0,0,1,2.3,1.86l1.36-.48a3.73,3.73,0,0,0-7.29.54,3,3,0,0,0,2.67,3l1.55.33c1.09.24,1.63.91,1.63,1.7,0,.95-.74,1.74-2.25,1.74a2.51,2.51,0,0,1-1.79-.63,2.45,2.45,0,0,1-.82-1.71l-1.45.46a3.79,3.79,0,0,0,4.08,3.24c2.32,0,3.76-1.52,3.76-3.22C78.65,12.59,77.63,11.45,75.82,11.05Z"/><path id="path26" class="cls-1" d="M83.25,16A2.33,2.33,0,0,1,81,13.41a2.31,2.31,0,0,1,2.24-2.59,1.93,1.93,0,0,1,2,1.57l1.31-.55a3.22,3.22,0,0,0-3.29-2.35,3.7,3.7,0,0,0-3.72,3.92,3.75,3.75,0,0,0,3.74,3.94A3.41,3.41,0,0,0,86.59,15l-1.28-.55A2,2,0,0,1,83.25,16Z"/><path id="path28" class="cls-1" d="M88.69,5.89a1,1,0,1,0,.75.29A1,1,0,0,0,88.69,5.89Z"/><polygon id="rect30" class="cls-1" points="87.99 9.72 89.42 9.72 89.42 17.11 87.99 17.11 87.99 9.72"/><path id="path32" class="cls-1" d="M14.58,8.87A4.1,4.1,0,0,0,15.8,6H14.31a2.62,2.62,0,0,1-.76,1.87L11.66,9.7l-2-2A2.61,2.61,0,0,1,9,6H7.53A4.09,4.09,0,0,0,8.74,8.87l1.87,1.87L8.74,12.61a4.08,4.08,0,0,0-1.21,2.92v2.29H9.18V16.73h1.66v1.09h1.64V16.73h1.65v1.09H15.8V15.53a4.11,4.11,0,0,0-1.21-2.92l-1.38-1.36-1,1.05,1.37,1.36a2.63,2.63,0,0,1,.77,1.58H9a2.68,2.68,0,0,1,.76-1.58Z"/><path id="path34" class="cls-1" d="M11.61,23.21a11.61,11.61,0,1,1,11.6-11.6A11.6,11.6,0,0,1,11.61,23.21Zm0-21.89A10.29,10.29,0,1,0,21.9,11.61,10.28,10.28,0,0,0,11.61,1.32Z"/></g></g></svg> </span> <span id="bechsci-tooltip-{{$ctrl.productId}}" class="bechsci-tooltip-icon" data-toggle="tooltip" ng-click="$ctrl.showBechSciTooltip()" close-tooltip="$ctrl.closeBechSciTooltip()" data-html="true" title="Clicking this link will redirect you to the <a href='https://www.benchsci.com/' target='_blank'> BenchSci</a> website that provides third-party scientific content. 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image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836278" data-index="38"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_10632_F6.small.jpg?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836277" data-index="39"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_10632_F4.small.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836276" data-index="40"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_10632_F3.small.jpg?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836275" data-index="41"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_10632_F1.small.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="620820" data-index="42"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_1949_zbc0141342890005.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="524834" data-index="43"> <img src="https://www.thermofisher.com/antibody/images/150/zbc0141342890007-2-20190221105219.jpg?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="524833" data-index="44"> <img src="https://www.thermofisher.com/antibody/images/150/zbc0141342890006-2-20190221105219.jpg?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="349960" data-index="45"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-23344956-PDI-zbc0141342890009.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="349958" data-index="46"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-23344956-PDI-zbc0141342890004.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="349957" data-index="47"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-23344956-PDI-zbc0141342890003.jpg?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="349956" data-index="48"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-23344956-PDI-zbc0141342890001.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836253" data-index="49"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_10595_F3.small.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="346812" data-index="50"> <img src="https://www.thermofisher.com/antibody/images/150/zbc0521190110003-20180918151446.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836210" data-index="51"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_10568_F7.small.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="352754" data-index="52"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-20667829-PDI-zbc0431033500007.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836836" data-index="53"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_11097_jlb0337-fig-0003.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836837" data-index="54"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_11097_jlb0337-fig-0004.jpg?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="953735" data-index="55"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_20766_JEM_20201916_Fig1.jpg?time=20240507" alt="PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="953982" data-index="56"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_20766_JEM_20201916_FigS1.jpg?time=20240507" alt="PDI Antibody in Immunohistochemistry (IHC)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="739392" data-index="57"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_8268_fncel-12-00450-g009.jpg?time=20240507" alt="PDI Antibody in Immunohistochemistry (IHC)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="364746" data-index="58"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-20638985-PDI-nihms-215207-f0003.jpg?time=20240507" alt="PDI Antibody in Immunohistochemistry (IHC)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="879348" data-index="59"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_17264_pone.0242960.g003.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="876146" data-index="60"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_7652_41419_2019_1402_Fig2_HTML.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="739394" data-index="61"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_7652_41419_2019_1402_Fig5_HTML.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="739393" data-index="62"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="731052" data-index="63"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_6819_12192_2018_964_Fig3_HTML.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="731051" data-index="64"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_6819_12192_2018_964_Fig2_HTML.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="731289" data-index="65"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_6496_41418_2018_197_Fig6_HTML.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="723646" data-index="66"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_6496_41418_2018_197_Fig3_HTML.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="730756" data-index="67"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_6701_nihms679942f1.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="333899" data-index="68"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-24406246-PDI-gr1(20365).jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="730743" data-index="69"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_6594_zbc0141342890002.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="620654" data-index="70"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_1760_nihms-327775-f0003.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="490228" data-index="71"> <img src="https://www.thermofisher.com/antibody/images/150/jlb0989-fig-0004-1-20190103084817.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="836638" data-index="72"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_10913_F6.small.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="349954" data-index="73"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-18426796-PDI-zbc0280840940006.jpg?time=20240507" alt="PDI Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="490227" data-index="74"> <img src="https://www.thermofisher.com/antibody/images/150/jlb0989-fig-0002-1-20190103084817.jpg?time=20240507" alt="PDI Antibody in Neutralization (Neu)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="880144" data-index="75"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_15962_FEBS_6339_f3.jpg?time=20240507" alt="PDI Antibody in Neutralization (Neu)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="617701" data-index="76"> <img src="https://www.thermofisher.com/antibody/images/150/MA3019-PDI-siRNA-AV-WB.jpg?time=20240507" alt="PDI Antibody"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="757950" data-index="77"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time=20240507" alt="PDI Antibody"/> </div> </div> </div> </div> </div> <noscript> <div itemscope itemtype="http://schema.org/ImageGallery"> <h2>Product Image Gallery</h2> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_7.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of Phalloidin (orange) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Colon-tissue.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))" title="PDI Antibody (MA3-019) in IHC (P)"> <figcaption itemprop="caption">Immunohistochemistry was performed on normal deparaffinized Human colon tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PDI (Product # MA3-019) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3-019_WB_20130731115533.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Western blot analysis of PDI was performed by loading 25 µg of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 57 kDa.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_3T3_20130708115155.jpg?time=20240507?time=20240507" alt="PDI Antibody in Flow Cytometry (Flow)" title="PDI Antibody (MA3-019) in Flow"> <figcaption itemprop="caption">Flow cytometry analysis of PDI in NIH/3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_6.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of Phalloidin (green) and PDI (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_8.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_NS-1.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in NS-1 Cells.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI_Antibody_MA3-019_Immunofluorescence_5.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/mL final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI-Antibody-MA3019-IF-9.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of PDI (red) in MDCK cells. The cells were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a PDI mouse monoclonal antibody (Product # MA3-019), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A27027) at a dilution of 1:1000 for 30 minutes at room temperature (red). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_HMVEC.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in HMVEC Cells.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_A549.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in A549 Cells.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/PDI_MA3-019_Immunofluorescence_p19.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product # MA3-019) shows staining in p19 Cells.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Lung-adenocarcinoma.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))" title="PDI Antibody (MA3-019) in IHC (P)"> <figcaption itemprop="caption">Immunohistochemistry was performed on cancer biopsies of deparaffinized Human lung adenocarcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PDI (Product # MA3-019) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3-019_Immunohistochemistry_Tonsil-tissue.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))" title="PDI Antibody (MA3-019) in IHC (P)"> <figcaption itemprop="caption">Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PDI (Product # MA3-019) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-PDI-WB.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Western blot was performed using Anti-PDI Mouse Monoclonal Antibody (Product # MA3-019) and a 57 kDa band corresponding to PDI was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2),NIH/3T3 (Lane 3), A-375 (Lane 4), Hep G2 (Lane 5), HL-60 (Lane 6), and PC-12 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-PDI-siRNA-WB.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Knockdown of PDI was achieved by transfecting A-431 cells with PDI specific siRNAs (Silencer® select Product # s441). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the PDI knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-PDI Mouse Monoclonal Antibody (Product # MA3-019, 1:2000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Densitometric analysis of this Western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PDI.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_K562_20130708115224.jpg?time=20240507?time=20240507" alt="PDI Antibody in Flow Cytometry (Flow)" title="PDI Antibody (MA3-019) in Flow"> <figcaption itemprop="caption">Flow cytometry analysis of PDI in K562 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3-019_FACS_Hela_20130708115131.jpg?time=20240507?time=20240507" alt="PDI Antibody in Flow Cytometry (Flow)" title="PDI Antibody (MA3-019) in Flow"> <figcaption itemprop="caption">Flow cytometry analysis of PDI in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_21318_urn-x-wiley-15387933-media-jth15740-jth15740-fig-0003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">3 FIGURE ER retention of VWF in ECFCs with VWF propeptide variants. A, Ex 4-5del; B, p.Cys633Arg; C, p.Arg273Trp; D, p.Pro293Glnfs*164 and p.Gln419* ECFCs. Co-staining of ECFCs for VWF (green) and the ER marker PDI (red) shows co-localization (yellow) indicating ER retention of the variant (diffuse) VWF in heterozygous and homozygous VWF propeptide ECFCs. In normal ECFCs (Unaffected III-5, III-6), VWF is in WPBs and not associated with the ER marker. Images were obtained using a Leica TCS SP8 microscope at 63x magnification, upgraded with Hyvolution software (scale bars = 50 mum; and 10 mum for zoomed in panels). White boxes show the magnified area. The optical zoom is provided at the top right corner of each zoomed in image. C. Het, compound heterozygous; ECFCs, endothelial colony-forming cells; ER, endoplasmic reticulum; Het, heterozygous; Hom, homozygous; PDI, protein disulfide-isomerase; VWF, von Willebrand factor; WPBs, Weibel-Palade bodies</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20021_spectrum.00422-21-f006.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">FIG 6 The LTM-1 and LTM-2 motifs of NTPase have the potential to direct fusion proteins to the ER. (A) Schematic diagram of various NTPase(1-179) deletion mutants. The ability and degree of NTPase(1-179)-GFP and its deletion mutants for LD localization and for ER localization are summarized. (B) Colocalization of NTPase(1-179) deletion constructs with LDs or with the ER in cells. A7 cells were transfected with the indicated expression plasmids for 24 h, and then the transfected cells were dually stained with LipidTOX and anti-PDI antibody; scale bars, 10 mum. The quantitative results are plotted as mean +- SEM ( n = 15 cells obtained from two independent experiments); *, P < 0.05 for results compared to those from the empty vector group (GFP); #, P < 0.05 for results compared to those from the NTPase(1-179)-GFP-transfected group. (C) Confocal microscopic analysis of the localization of NTPase(1-179)-GFP in cells at different time points (6, 18, and 24 h) after transfection. A7 cells were transfected with the NTPase(1-179)-GFP expression plasmid for 6, 18, or 24 h, and then the subcellular localization of this protein to LDs or the ER in transfected cells was analyzed by confocal microscopy; scale bars, 10 mum. The quantitative results are plotted as mean +- SEM ( n = 15 cells obtained from two independent experiments); *, P < 0.05 for results compared to those from the 6 h group.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20102_MGG3-9-e1777-g002.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">FIGURE 4 The intracellular localization of CatD and enzyme activity in No. 1002 cells. (a) The immunofluorescence cell localization of wild-type and mutant CatD expressed in No. 1002 cells. After transfecting vector, wild-type, and mutant plasmids into No. 1002 cells, the co-localization of the CatD protein (green) and lysosome (red), as well as the co-localization of the CatD protein (green) and endoplasmic reticulum (red) were observed. (b) The ratio of mature-CatD and pro-CatD in No. 1002 cells transfected with the wild-type and mutant of the CTSD gene, respectively. (c) The CatD activity of wild-type and mutant No. 1002 cells. CatD activity expressed as relative fluorescence units (RFU) per million cells</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17583_mbc-32-1594-g005.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">FIGURE 5: RUSH assay reveals mislocalization of B4GalT1 to endolysosomes in GARP-KO RPE1 cells. RPE1 cells were transfected with plasmids encoding Str-KDEL_flB4GalT1-SBP-mCherry in biotin-free medium. Following overnight incubation, biotin (40 uM) and cycloheximide (50 uM) were added for 0, 2, and 6 h. (A) Colocalization of B4GalT1 with ER marker PDI at time 0. (B, C) Colocalization of B4GalT1 with Golgi protein GM130 at 2 h (B) and 6 h (C). The graph on the right side of C shows the quantification of B4GalT1 and GM130 colocalization at 6 h of biotin/cycloheximide addition. Values in the bar graph represent the mean +- SD of the colocalization between B4GalT1 and GM130 in approximately 40 cells in WT and VPS54-KOs. Colocalization in VPS53-KO cells was measured using each field in approximately 20 cells. Statistical significance was calculated using one-way ANOVA. **** P <= 0.0001. (D) Colocalization of B4GalT1, GM130 and the endolysosomal marker CD63 after addition of biotin/cycloheximide mix for 6 h. Quantification of the colocalization between CD63 and B4GalT1 was done by Pearson's correlation coefficient analysis in approximately 25 cells. Statistical significance was calculated using one-way ANOVA. **** P <= 0.0001, * P <= 0.05.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12377_viruses-13-00216-g003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Figure 3 NS3 localizes to the ER when NS2B is provided in trans and in cis. ( A ) HEK293T cells were co-transfected with NS3-GFP and NS2B-V5 expressing plasmids ( a - c ). At 24 h post-transfection, cells were fixed and co-stained with anti-GFP and antibodies against PDI (red; a ), giantin (red; b ) or tubulin (red; c ). HEK293T cells were also triple-transfected with NS3-GFP, NS2B-V5/His and IKKepsilon-FLAG expressing plasmids ( d ) and triple stained with anti-GFP (green; d ), anti-V5/His (red; d ) and anti-IKKepsilon (blue; d ) antibodies. ( B ) HEK293T cells were transfected with NS2B-NS3-GFP ( a - c ) or co-transfected with NS2B-NS3-GFP and IKKepsilon-FLAG expressing plasmids ( d ) and processed for immunofluorescence at 24 h after transfection using antibodies against PDI (red; a ), giantin (red; b ), tubulin (red; c ) and IKKepsilon (red; d ). The detected viral and host proteins are listed on the top right corner of each panel. White arrows indicate colocalization between the GFP-tagged viral protein and the ER marker. Nuclear DNA was labeled with DAPI. Scale bar represents 10 mum. The main panels depict merged images with the side panels showing the individual channels. The transfection efficiency (TE), the co-transfection efficiency (Co-TE) and the triple-transfection efficiency (Triple-TE) for cells expressing one, two, or three gene constructs, respectively, are listed at the bottom of the panel. Images are representative of two independent transfection experiment</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12377_viruses-13-00216-g002.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Figure 2 WNV NS3 is distributed throughout the cytoplasm, while NS2B remains in the ER in transfected cells. ( A ) NS3-GFP transfected ( a - c ) or NS3-GFP and IKKepsilon-FLAG co-transfected ( d ), HEK293T cells were fixed at 24 h post-transfection and immunostained with antibodies against protein disulfide isomerase (PDI), an ER marker, (red; a ), anti-GM130, a Golgi apparatus marker, (red; b ), anti-tubulin, a cytoskeletal marker, (red; c ), or anti-IKKepsilon, a soluble cytoplasmic protein marker, (red; d ). ( B ) NS2B-V5/His transfected ( a - c ) or NS2B-V5/His and IKKepsilon-FLAG co-transfected ( d ) HEK293T cells were fixed 24 h after transfection and immunolabeled with rabbit anti-PDI (green; a ), anti-giantin (green; b ), anti-tubulin (green; c ) or anti-IKKepsilon (green; d ) antibodies to visualize the ER, Golgi apparatus, cytoskeleton or the cytoplasmic protein kinase IKKepsilon protein, respectively. Visualized viral and host proteins are listed on the top right corner of each panel. Nuclear DNA was labeled with DAPI. Confocal microscopy images were of optical slice thickness ~1 mum. Scale bar, 10 mum. The main image depicts the merged image from three channels separately shown on the side. White arrows indicate colocalization between the green and red channels. The transfection efficiency (TE) for cells transfected with one construct or the co-transfection efficiency (Co-TE) for cells co-expressing the viral protein and IKKepsilon are listed at the bottom of each</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17116_fcell-08-00414-g003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">FIGURE 3 Confocal analysis of the subcellular localization of FAM46A in transfected K562 cells. Cells were transfected with an expression construct encoding the FAM46A-EGFP protein, which was detected in the nuclear (A) and cytoplasmic (B) compartments. Within the nucleus (A) , FAM46A was predominantly confined in the chromatin domains more accessible to the transcription complexes (as indicated by the acK9-Histone H3 staining) and were mostly excluded from the regions with condensed chromatin structures (as indicated by the meK9-Histone H3 staining). In addition, significant co-localization of FAM46A with NuMA and lamin A/C was observed. DAPI staining defined the morphology of nuclei. Scale bar: 5 mum. Within the cytoplasm (B) , the majority of FAM46A was found to co-localize with the endoplasmic reticulum marker, protein disulfide-isomerase (PDI). By contrast, co-localization of FAM46A with trans-Golgi network marker gamma-adaptin (AP-1) and early endosome marker transferrin receptor (TrfR) is relatively low. DAPI staining defines the morphology of nuclei. Scale bar: 5 mum.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19457_gr6_lrg.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Fig. 6 Co-immunostaining of CypB and cell organelles in HCoV-229E-infected Huh7 cells . For the identification of the intense cyclophilin B bleb-like structures infected cells (MOI = 1; 48 h p.i.) were co-stained with anti-CypB and antibodies directed against markers of the ER (anti-PDI), cis-GOLGI (anti-GM130), autophagosomes (anti-SQSTM1), anti-P-bodies (anti-hDcp1a) and stress granules (anti-PABP). Cyclophilin B normally distributes within the ER. In the presence of HCoV-229E, it intriguingly concentrates at bleb-like structures of the ER. Fig. 6</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_7658_41467_2019_9549_Fig6_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Fig. 6 Disruption of ER-Golgi trafficking disassembles vRNP hotspots. A549 cells were infected or mock-infected with PR8 virus at an MOI of 3. a At 90 min p.i., 2 mug mL -1 of brefeldin A (BFA) or 10 mug mL -1 nocodazole (NOC) were added and incubated for 10 h until the supernatant was collected and viral titres determined or cells were harvested in Laemmli's and NP levels detected by western blotting. Statistical analysis of data was performed using a non-parametric one-way ANOVA, followed by Friedman's multiple comparisons test (* p < 0.05). b - d At 8 hpi, cells were also treated or mock-treated with 2 mug mL -1 of BFA for 1 h. b Cells were immunostained for the ER marker PDI (in green), the viral protein NP (in red) and the cis -Golgi marker GM130 (in gray) and imaged by confocal microscopy. Selected areas of the cytoplasm are marked by white boxes and displayed on the top left corner of the images. Bar = 10 mum. c The frequency distribution of NP inclusions within the three size categories (in mum 2 ), the number of inclusions per mum 2 , and the percentage of NP staining that is inside inclusions were plotted for each condition. Statistical analysis of data was performed using a non-parametric Kruskal-Wallis test, followed by Dunn's multiple comparisons test (*** p < 0.001). An average of 60 cells from 2 independent experiments was analyzed per condition. d Infected cells were stained for the host protein Rab11 (in green) and the viral proteins NP (in re</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_7658_41467_2019_9549_Fig7_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Fig. 7 Disruption of ER exit sites dissolves viral inclusions. HeLa cells were transfected with plasmids encoding GFP, SAR1 WT-GFP (SAR1) or SAR1-GTP-GFP (SAR1-GTP) and, 24 h later, infected or mock-infected with PR8 virus, at an MOI of 10. At 16 hpi, cells were fixed and processed for immunofluorescence. a Cells were stained for the viral protein NP (in red) and for the ER protein PDI (in gray). Areas highlighted by the white box are shown on the right of each panel. b The number of inclusions per mum 2 , the percentage of NP staining that is inside inclusions and the frequency distribution of NP inclusions within the three area categories (in mum 2 ) were plotted for each condition. Statistical analysis of data was performed using a non-parametric Kruskal-Wallis test, followed by Dunn's multiple comparisons test (*** p < 0.001). More than 40 cells from 2 independent experiments were analyzed per condition. c Infected cells were stained for the viral protein HA and imaged by confocal microscopy. Bar = 10 mum</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10952_F6.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6358_zbc0421894430006.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/41598_2018_23483_Fig4_HTML-1-20190221105219.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Figure 4 Search for potential mechanisms involved in Brucella abortus -induced mitochondrial fragmentation ( a ). TOM20 immunostaining in HeLa cells pre-incubated for 30 min with the indicated concentration of ruthenium red and infected with B. abortus 2308 mCherry with or without the inhibitor - 48 h PI (Representative of n = 3). Green: TOM20 (Alexa488)/Red: B. abortus 2308 (mCherry)/Turquoise: Nuclei (Hoechst) ( b , c ). Aspect ratio ( b ) and end point/branch point ratio ( c ) of HeLa cells pre-incubated for 30 min with the indicated concentration of ruthenium red and infected (white) or not (black) with B. abortus 2308 mCherry with or without the inhibitor - 48 h PI. Results represent means +- SD for three independent experiments (n = 3). Statistical analysis: two-way ANOVA on Box Cox transformed data. P value for interaction: 0.446 and 0.051 respectively. The numbers indicated in the columns represent the number of cells analysed for each condition. ( d ) PDI and TOM20 co-immunostaining in HeLa cells infected or not (control) with B. abortus 2308 mCherry - 48 h PI (Representative of n = 3). Green: PDI (Alexa488)/Blue: TOM20 (Alexa633)/Red: B. abortus 2308 (mCherry)/Turquoise: Nuclei (Hoechst). ( e ) Western blot analysis of P-IRE1 and IRE1 abundance in RAW 264.7 macrophages infected or not with B. abortus 2308 mCherry - 48 h PI (Representative of n = 3 in different conditions). RAW 264.7 cells treated for 6 h with 10 uM tunicamycin and then left for 18 h for</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/41598_2017_16947_Fig8_HTML-20180918151446.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_4170_zjv0041833180003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">FIG 3 N-terminal 179-aa region of GII-NTPase is required for vesicle formation and ER colocalization. (A) Schematic diagram of the full-length and deletion constructs of F-NTPase. The abilities of F-NTPase and its deletions to promote vesicle formation and ER colocalization are summarized in the diagram. (B) Western blot analysis of F-NTPase and its deletions expressed in 293T cells. (C) Representative confocal immunofluorescence images of F-NTPase constructs (green) and PDI (red) in A7 cells. White arrows indicate the positions of vesicle-like structures formed by F-NTPase or its deletions. Yellow arrows represent the colocalization between the indicated F-NTPase constructs and PDI in the merged images. Scale bars, 10 mum.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-28489076-PDI-ncomms15244-f4.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Figure 4 Subcellular localization of DP4 and DP5 in the presence or absence of Ii. ( a - c ) HEK293 cells were transfected with the indicated combinations of genes. Fixed cells were permeabilized and stained for DP (red) in conjunction with an ER marker, PDI (green, left panels), an early endosomal marker, EEA1 (green, middle panels) or a late endosomal/lysosomal marker, LAMP-1 (green, right panels) and analysed by confocal microscopy. Inset boxes indicate the areas depicted at higher magnification. Scale bar in all images, 10 mum. Quantification of co-localized spots represents means+-s.d. of three counted cells in each condition. ns, not significant; * P <0.05, ** P <0.01, *** P <0.001 by unpaired, two-tailed Welch's t -test.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11105_F5.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-26089538-PDI-1583fig5.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10632_F9.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10632_F7.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)" title="PDI Antibody (MA3-019) in WB, ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot and Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10632_F6.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)" title="PDI Antibody (MA3-019) in WB, ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot and Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10632_F4.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10632_F3.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)" title="PDI Antibody (MA3-019) in WB, ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot and Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10632_F1.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_1949_zbc0141342890005.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/zbc0141342890007-2-20190221105219.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)" title="PDI Antibody (MA3-019) in WB, ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot and Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/zbc0141342890006-2-20190221105219.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)" title="PDI Antibody (MA3-019) in WB, ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot and Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-23344956-PDI-zbc0141342890009.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-23344956-PDI-zbc0141342890004.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-23344956-PDI-zbc0141342890003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)" title="PDI Antibody (MA3-019) in WB, ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot and Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-23344956-PDI-zbc0141342890001.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10595_F3.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/zbc0521190110003-20180918151446.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10568_F7.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-20667829-PDI-zbc0431033500007.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11097_jlb0337-fig-0003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">3 (A) Western blot analysis of HEK293 cells transfected with mDC-STAMP-GFP constructs. (Lane 1) HEK293 cells; (lane 2) HEK293/GFP; (lane 3) HEK293/mDC-STAMP-GFP; and (lane 4) HEK293/mDC-STAMP-GFP plus tunicamycin. Western blots were stained with anti-GFP mAb. The arrow indicates unglycosylated mDC-STAMP-GFP. (B) HEK293 cells, stably transfected with mDC-STAMP-GFP or hDC-STAMP-GFP constructs, respectively, were stained with antibodies against beta1 integrins, against the protein present in the ER (anti-PDI), and the intermediate compartment (anti-ERGIC-53). Cells adherent to the fibronectin-coated slides were visualized by CLSM for GFP autofluorescence (green) and the markers (blue) as indicated. (C) Immunoelectron microscopy of HEK293 cells stably transfected with hDC-STAMP-GFP. Sections were stained with the antibody against GFP and incubated with the 10-nm gold beads. The arrows point to the gold beads at the nuclear membrane (left) and in the ER (right). The other gold particles in the left panel are located on ER structures, as is more clearly shown in the right panel. No staining was visible at the mitochondria (M) or in the nucleus (N). Original magnification, 20,000x.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11097_jlb0337-fig-0004.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunocytochemistry (ICC/IF)" title="PDI Antibody (MA3-019) in ICC/IF"> <figcaption itemprop="caption">4 DC-STAMP-GFP localizes to the ER in DC. Mo-IDC transduced with the adenovirus Ad5fib35 encoding hDC-STAMP-GFP (green) were stained with a beta2-integrin antibody (red), an ER marker (anti-PDI, blue), and an intermediate compartment marker (anti-ERGIC-53, blue), and fluorescence was visualized by CLSM.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20766_JEM_20201916_Fig1.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))" title="PDI Antibody (MA3-019) in IHC (P)"> <figcaption itemprop="caption">Figure 1. EN1 expression is up-regulated in fibrotic skin. (A) Representative immunofluorescence stainings for EN1 (green) and costainings with P4H (red) and CD45 (magenta) in the dermis of healthy donors and SSc patients at 1,000-fold magnification (scale bars = 20 um). Hematoxylin and eosin stainings (200-fold magnification, scale bars = 100 um), Voronoi diagrams, and percentages of EN1-positive fibroblasts from all fibroblasts (defined as P4H + CD45 - cells) are included. Data are shown from one experiment ( n >= 4). (B and C) EN1 mRNA (B) and EN1 intracellular or secreted protein levels (C) in cultured fibroblasts or their supernatants from healthy donors and SSc patients. Representative Western blot images and quantifications are included (C). Data are shown from one experiment ( n = 3). (D and E) En1 protein levels in the skin of mice challenged with bleomycin (D) or Tsk-1 mice (E) and their respective controls. Representative Western blot images and quantifications are included. Data are compiled from two independent experiments ( n >= 4). (F and G) Representative immunofluorescence stainings for EN1 (green) and costainings with Vimentin (red) and CD45 (magenta) in the dermis of mice challenged with bleomycin and their controls at 1,000-fold magnification (F; scale bars = 20 um) and in the hypodermis of Tsk-1 mice and their controls, respectively, at 1,000-fold magnification (G; scale bars = 20 um). Hematoxylin and eosin stainings (100-fold magnification, scale bars =</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20766_JEM_20201916_FigS1.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunohistochemistry (IHC)" title="PDI Antibody (MA3-019) in IHC"> <figcaption itemprop="caption">Figure S1. EN1 expression in healthy and SSc skin. (A-C) Representative immunofluorescence stainings for EN1 (green) and costainings with CD31 (A) and CD45 (B; both magenta) in the dermis of healthy donors and SSc patients at 1,000-fold magnification (scale bars = 20 um). Hematoxylin and eosin stainings (200-fold magnification, scale bars = 100 um) are included. Data for A-C were obtained from n >= 4 biological samples from two independent experiments. (D) Composition of clusters from the segmented IMC images in ventral and dorsal skin biopsies from SSc patients. (E) tSNE plot showing distribution of cells from ventral and dorsal SSc skin across clusters. (F) tSNE plot showing expression of EN1 across the ventral and dorsal SSc clusters. Data for D-F were obtained from n = 8 biological samples from four SSc patients (paired ventral and dorsal biopsies).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8268_fncel-12-00450-g009.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunohistochemistry (IHC)" title="PDI Antibody (MA3-019) in IHC"> <figcaption itemprop="caption">FIGURE 9 Immunohistochemical localization of m2R in neuronal compartments involved in endocytosis, synthesis, maturation, and degradation in fixed hippocampal neurons. Hippocampal neurons were transfected with m2R-WT. Neurons were stimulated with CCh at 30 muM for 6, 20 min and 1 h fixed, and processed for visualization of m2R together with markers of intraneuronal compartments and observed by confocal microscopy. ( A-C) 6 min after CCh stimulation (30 muM), some m2R immunopositive punta colocalize with CHC in clathrin-coated pits, EEA1 in early endosomes and Rab9 in late endosomes (arrow heads). (D-E) Twenty minutes after CCh stimulation (30 muM), we failed to detect no colocalization of m2R with PDI, a marker of endoplasmic reticulum and GM130, a marker of Golgi apparatus. (F-F) One hour after CCh stimulation (30 muM), some m2R immunopositive puncta colocalize with CathD, a marker of lysosomes (arrow heads). The quantitative analysis of the colocalization of m2R and markers of subcellular compartment in neurons was performed using the Jacop Plugin of ImageJ and statistical data are reported from the Costes's randomization-based colocalization module (see Materials and Methods). Data are expressed as a Pearson's coefficient (pc) and pc were compared using the Mann-Whitney U -test. Our analysis shows that the colocalization of the immunofluorescent signals for m2R with CHC, EEA1, Rab9, and CathD is higher after treatment with CCh compared to untreated neurons (CHC, Rab9</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-20638985-PDI-nihms-215207-f0003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Immunohistochemistry (IHC)" title="PDI Antibody (MA3-019) in IHC"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17264_pone.0242960.g003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">10.1371/journal.pone.0242960.g003 Fig 3 Endoplasmic reticulum chaperones are downregulated in CTEPH-EC. (A) Representative blot of the GRP94 expression in static, low, and high magnitude shear stress (0, 5, and 15 dynes/cm 2 ). Data are presented as the mean +- standard error: (*) HPAEC vs CTEPH-EC, (&) HPAEC 15 vs CTEPH-EC 15. (n = 5-7). (B) GRP78 and PDI expression before and after shear stress exposure as described in methods. Data are presented as the mean +- standard error: (*) HPAEC vs HPAEC 15, (#) CTEPH-EC vs CTEPH-EC 5 (&) HPAEC 15 vs CTEPH-EC 15, P < 0.05. (n = 6-7). All of the samples were normalized to ss-actin and run in the same gel.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig2_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Fig. 2 Nox NADPH oxidase expression and superoxide generation by HCT116 cells with overactivated KRas. a Nox1, NoxA1, p67phox, NoxO1, and p47phox basal protein expression by western analysis normalized for GADPH. Nox1 immunoblot densities were quantified using Odyssey software. * P < 0.05, ANOVA plus Tukey's multiple comparisons test ( n = 3). b RhoGDIalpha, RhoA, and Rac1 basal protein expression by western analysis normalized for GAPDH. Rac1 immunoblot densities were quantified using Odyssey software. ** P < 0.01; *** P < 0.005, ANOVA plus Tukey's multiple comparisons test ( n = 3). c Nox1 complex scheme. d Rac1 basal activity, using Rac1 G-LISA activation assay (Cytoskeleton,inc). Test t * p < 0.05, ( n = 2). e Rac1 protein expression by western analysis after 72 h of PDIA1 silencing. Rac1 Immunoblot densities were quantified using Odyssey software ** P < 0.01; ns: not significant, Student's t -test ( n = 3). f Western analysis of NoxO1 and RhoGDIalpha protein expression before and after PDIA1 silencing, normalized for GAPDH. RhoGDIalpha immunoblot densities were quantified using Odyssey software, **** P < 0.001; ns: not significant, Student's t -test ( n = 3). g Superoxide production after 72h of PDIA1 silencing in HCT116 cells treated or not with 10 uM of NoxA1ds Nox1's peptide inhibitor. EOH 2-hidroxyethidiium relative superoxide production, si-PDI, si-RNA against PDIA1 protein; Scrmb NoxA1ds, NoxA1ds negative control peptide; NoxA1ds, Nox1 peptide inhibitor. * P < 0.05</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig5_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Fig. 5 Effects of PDIA1 silencing in signaling targets and EMT marker expression. PathScan array analysis. All array's spots were quantified and analyzed using ImageJ software. PDIA1 silencing was checked by immunoblot analysis. Ctrl, si-RNA negative control; si-PDI, si-RNA against PDIA1; ERK1/2, Thr202/Tyr204 phosphorylation; Stat1, Tyr701 phosphorylation; Stat3, Tyr705 phosphorylation; Akt, Thr308 phosphorylation; Akt, Ser473 phosphorylation; AMPKa, Thr172 phosphorylation, s6 ribosomal protein, Ser235/236 phosphorylation; mTOR, Ser2448 phosphorylation; HSP27, Ser78 phosphorylation; Bad, Ser112 phosphorylation; p70 S6 kinase, Thr389 phosphorylation; PRAS40, Thr246 phosphorylation; p53, Ser15 phosphorylation; p38, Thr180/Tyr182 phosphorylation; SAPK/JNK, Thr183/Tyr185 phosphorylation; PARP, Asp214 cleavage; Caspase-3, Asp175 Cleavage; GSK-3beta, Ser9 phosphorylation. b GSK-3beta, Ser9 phosphorylation plot. c Stat3, Tyr705 phosphorylation. d Basal E-cadherin (E-cad) protein expression by immunoblot normalized for GAPDH ( n = 3). e Immunoblot for E-cadherin before and after PDIA1 silencing in Caco2 cells ( n = 2). f Immunoblot for E-cadherin before and after PDIA1 silencing in HKE3 cells.; ns: not significant, Student's t test ( n = 3). g Immunoblot for E-cadherin before and after PDIA1 silencing in HCT116 cells * P < 0.05, Student's t test ( n = 3). Immunoblot densities were quantified using Odyssey software</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Fig. 1 Role of PDIA1 in oxidant generation by colon carcinoma cells with distinct levels of KRas activation. a KRas expression and activity in Caco2, HKE3, and HCT116 cells. Active KRas was pulled down with GST-RBD beads from lysates of serum-starved cells treated with EGF 25 ng/mL for 10 min. Aliquots of the lysates were blotted for total KRas and GAPDH as loading control. b RNAseq analysis of P4HB (PDIA1) gene in Caco2, HCT116, and HCT15 cells, showing mean expression in FPKM (Fragments Per Kilobase Million), P4HB fold-change expression in HCT15 vs. Caco2 (q < 0.000001) and HCT116 vs. Caco2 (q < 0.000001). c PDIA1 basal protein expression by western analysis, normalized for GAPDH ( n = 3) and quantified using Odyssey software. d Intracellular PDIA1 titration, using Human P4HB ELISA Pair Set (SinoBiological). Test t ** p < 0.01, ( n = 4); e-g ROS production after 72 h of PDIA1 silencing, measured by HPLC analysis of DHE oxidation products. DHE oxidation produces, among many others, two major products: 2-hidroxyethidium (EOH), which is representative of superoxide species and ethidium, representative of other oxidant species. Ctrl negative, si-RNA control; si-PDI, si-RNA against PDIA1 protein. Representative immunoblots of PDI silencing for each cell type Test t * p < 0.05; ** p < 0.01. For Caco2 cells, n = 3; HKE3 cells, n = 4; HCT116 cells, n = 5</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6819_12192_2018_964_Fig3_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6819_12192_2018_964_Fig2_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6496_41418_2018_197_Fig6_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Fig. 6 Caveolin-1 expression precludes DRP1 phosphorylation and redistribution during the early phase of ER stress. a Mock and CAV1 cells in control condition (con) or under early ER stress with tunicamycin (tun) were fractionated and processed for detection of phospho-DRP1 and DRP1 by western blotting using PDI as a loading control. C cytosol, LM light membranes (microsomes), HM heavy membranes (crude mitochondria). b Quantification of DRP1 phosphorylation analysed in ( a ) ( n = 3). c Quantification of membrane-bound DRP1 distribution analysed in a ( n = 3). d Experimental groups as in ( a ) were processed for immunofluorescence analysis. CAV1, mtHSP70 (mitochondrial marker, Mito) and DRP1 were stained with respective antibodies and then imaged using confocal microscopy. Merge between DRP1 and CAV1 images shows colocalized areas in yellow. Merge between DRP1 and Mito images shows colocalized areas in cyan. In merge images of Mito, DRP1 and CAV1, triple colocalization areas appear as white pixels. e CAV1 fluorescence per cell was quantified from images obtained in d ( n = 3). f DRP1-CAV1 colocalization was quantified as Manders' coefficients of images obtained in ( d ) ( n = 3). g DRP1-Mito colocalization was quantified as Manders' coefficients of images obtained in ( d ) ( n = 3). h From images obtained in d , thresholded CAV1 (C) and Mito (M) images were generated. The intersection between M and DRP1 corresponds to mitochondrial DRP1 (DRP1 mito ). Merge between M and C ima</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6496_41418_2018_197_Fig3_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Fig. 3 Caveolin-1 accumulates in mitochondria-associated ER membranes, precluding their remodelling upon early-phase ER stress. a Wild-type HeLa cells were labelled by immunogold staining with antibodies against CAV1. Blue arrowheads indicate CAV1 signals at the plasma membrane; red arrowheads indicate CAV1 signals at ER cisternae; yellow arrowheads indicate CAV1 at the ER-mitochondria interface. In the lower right panel, the ER is highlighted in red and mitochondria (mito) in green. Scale bar: 1 um. b Cells lysates were fractionated by differential sedimentation. HM heavy membranes (crude mitochondria), LM light membranes (microsomes). c HeLa mock and CAV1 cells in control condition (con) or under early ER stress with tunicamycin (tun) were processed and fractionated as in b . CAV1 levels were analysed by western blotting using PDI as a loading control. d Quantification of membrane-bound CAV1 analysed in c ( n = 3). e HM fraction obtained as in ( b ) was separated into MAM and pure mitochondria (mito) fractions by Percoll density gradient centrifugation. f Markers of cell fractionation procedures shown in b and e : FACL4 short isoform (mito), long isoform (MAM); COX IV (mito), Calnexin (CNX) (LM and MAM), SERCA2b (LM) and PDI (C, LM and HM). g Experimental groups as in c were processed and fractionated as in e . CAV1 levels were analysed by western blotting using PDI as a loading control. h Quantification of CAV1 in MAM analysed in g ( n = 3). i Experimental groups as in c w</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6701_nihms679942f1.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-24406246-PDI-gr1(20365).jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Fig. 1 Western blot analysis of Cyp3a2, Sec61alpha, Oatp1a1, Ntcp, Mrp2, and PDI in SER and RER isolated from Wistar and TR - rat livers. 5 mug (Cyp3a2, Mrp2), 50 mug (Sec61alpha) or 100 mug (Oatp1a1, Ntcp) protein was loaded per lane and separated by SDS-PAGE as given in section 2.4. Samples were probed against Cyp3a2, Sec61alpha, Oatp1a1, Ntcp, Mrp2, and PDI as described in 2.4. PDI was used as loading control in SER and RER fractions. Basolateral membrane fraction (BLM) isolated from Sprague Dawley rat liver was used as positive control for Oatp1a1 and Ntcp expression. Canalicular (CM) membrane fraction isolated from Sprague Dawley rat liver was used as positive control for Mrp2 expression. The figure shows a representative Western blot of at least two independent SER and RER isolations.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6594_zbc0141342890002.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_1760_nihms-327775-f0003.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/jlb0989-fig-0004-1-20190103084817.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10913_F6.small.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-18426796-PDI-zbc0280840940006.jpg?time=20240507?time=20240507" alt="PDI Antibody in Western Blot (WB)" title="PDI Antibody (MA3-019) in WB"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/jlb0989-fig-0002-1-20190103084817.jpg?time=20240507?time=20240507" alt="PDI Antibody in Neutralization (Neu)" title="PDI Antibody (MA3-019) in Neu"> <figcaption itemprop="caption">Published figure using PDI monoclonal antibody (Product # MA3-019) in Neutralization</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_15962_FEBS_6339_f3.jpg?time=20240507?time=20240507" alt="PDI Antibody in Neutralization (Neu)" title="PDI Antibody (MA3-019) in Neu"> <figcaption itemprop="caption">3 Increased binding of vitronectin and LM609 to Mn 2+ -stimulated endothelial cells requires free thiols in alpha V beta 3 and active PDI. Endothelial cells were stimulated with Mn 2+ , and their binding of LM609 (A, B) and vitronectin (C, D) was evaluated by flow cytometry in the absence or presence of the membrane-impermeant sulfhydryl group reagent PCMBS, a PDI-specific inhibitor (A, C), bacitracin (B), or blocking antibody to PDI (mAb 019) (D).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA3019-PDI-siRNA-AV-WB.jpg?time=20240507?time=20240507" alt="PDI Antibody" title="PDI Antibody (MA3-019)"> <figcaption itemprop="caption">Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with PDI siRNA and reduction of signal was observed in Western Blot using PDI Monoclonal Antibody (Product # MA3-019). {KD}</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_7652_41419_2019_1402_Fig1_HTML.jpg?time=20240507?time=20240507" alt="PDI Antibody" title="PDI Antibody (MA3-019)"> <figcaption itemprop="caption">Fig. 1 Role of PDIA1 in oxidant generation by colon carcinoma cells with distinct levels of KRas activation. a KRas expression and activity in Caco2, HKE3, and HCT116 cells. Active KRas was pulled down with GST-RBD beads from lysates of serum-starved cells treated with EGF 25 ng/mL for 10 min. Aliquots of the lysates were blotted for total KRas and GAPDH as loading control. b RNAseq analysis of P4HB (PDIA1) gene in Caco2, HCT116, and HCT15 cells, showing mean expression in FPKM (Fragments Per Kilobase Million), P4HB fold-change expression in HCT15 vs. Caco2 (q<0.000001) and HCT116 vs. Caco2 (q<0.000001). c PDIA1 basal protein expression by western analysis, normalized for GAPDH (n=3) and quantified using Odyssey software. d Intracellular PDIA1 titration, using Human P4HB ELISA Pair Set (SinoBiological). Test t **p<0.01, (n=4); e-g ROS production after 72 h of PDIA1 silencing, measured by HPLC analysis of DHE oxidation products. DHE oxidation produces, among many others, two major products: 2-hidroxyethidium (EOH), which is representative of superoxide species and ethidium, representative of other oxidant species. Ctrl negative, si-RNA control; si-PDI, si-RNA against PDIA1 protein. Representative immunoblots of PDI silencing for each cell type Test t *p<0.05; **p<0.01. For Caco2 cells, n=3; HKE3 cells, n=4; HCT116 cells, n=5 {KD}</figcaption> </figure> </div> </div> </noscript> <div id="tf-related-products-container-new"></div> <div class="product-details"> <div class="container"> <div class="row"> <div class="large-span-12 medium-span-12 small-span-12"> <h2 class="pdp-sub-heading product_details_heading">Product Details</h2></div> <div class="hidden-sku"> <h2>MA3-019</h2> </div> <div class="product-detail-section tested-app-section new-tbl-design product-detail-section table-spec"> <div class="product-container-head"> <div class="product-item"> Applications </div> <div class="product-item dilution-lbl-head "> Tested Dilution </div> <div class="product-item published-applications"> Publications </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Western Blot (WB)</h2></div> <div class="product-item "> <span class="dilution-lbl">1:200-1:2,000</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#WB-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 22 publications</span> <span class="mobile-view">22 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (IHC)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHC-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 12 publications</span> <span class="mobile-view">12 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (Paraffin) (IHC (P))</h2></div> <div class="product-item "> <span class="dilution-lbl">1:100</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHCP-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (Frozen) (IHC (F))</h2></div> <div class="product-item "> <span class="dilution-lbl">1:100</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHCF-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunocytochemistry (ICC/IF)</h2></div> <div class="product-item "> <span class="dilution-lbl">1:100</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#ICCIF-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 41 publications</span> <span class="mobile-view">41 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Flow Cytometry (Flow)</h2></div> <div class="product-item "> <span class="dilution-lbl">0.5-1 µg/test</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#Flow-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunoprecipitation (IP)</h2></div> <div class="product-item "> <span class="dilution-lbl">Assay-dependent</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IP-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 3 publications</span> <span class="mobile-view">3 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Neutralization (Neu)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#Neu-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 5 publications</span> <span class="mobile-view">5 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunomicroscopy (IM)</h2></div> <div class="product-item "> <span class="dilution-lbl">Assay-dependent</span> </div> <div class="product-item"> <span class="dilution-lbl dilution-lbl-dash">-</span> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IM-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view"></span> <span class="mobile-view"></span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Competition Assay (CA)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#CA-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> </div> <div class="product-detail-section tested-app-section new-tbl-design product-specific-section table-spec"> <div class="product-container-head"> <div class="product-item">Product Specifications</div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Species Reactivity</h2> </div> <div class="product-item left-flex-cls"> <span title="Cricetinae">Hamster,</span> <span title="Homo sapiens">Human,</span> <span title="Mus musculus">Mouse,</span> <span title="Suidae">Pig,</span> <span title="Rattus">Rat</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Published species</h2> </div> <div class="product-item left-flex-cls"> <span>Hamster,</span> <span>Human,</span> <span>Mouse,</span> <span>Non-human primate,</span> <span>Rat,</span> <span>Rhesus monkey</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2> Host/Isotype</h2> </div> <div class="product-item left-flex-cls"> <span title="Mus musculus">Mouse / IgG2a</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Class</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Monoclonal </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Type</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Antibody </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Clone</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> RL90 </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Immunogen </h2> </div> <div class="product-item left-flex-cls">Purified rat PDI protein. </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Conjugate</h2> </div> <div class="product-item left-flex-cls"> <a ng-if="$ctrl.checkConjugate('Unconjugated ') && ''.trim() != ''" href="" ng-attr-target="{{$ctrl.getRequestCustomLinkTarget()}}" class="conjugate-value">Unconjugated </a> <span ng-if="($ctrl.checkConjugate('Unconjugated ') && ''.trim() == '')" class="conjugate-value">Unconjugated </span> <span ng-if="!$ctrl.checkConjugate('Unconjugated ')" class="conjugate-value">Unconjugated </span> <div class="dropdown conjugate-dropdown"> <button class="btn-link conjugate-btn" type="button" data-toggle="dropdown"> View additional formats <span class="caret"></span> </button> <ul class="dropdown-menu dropdown-menu-fixed"> <li><a class="conjugate-color-padding" href="/antibody/product/MA3-019-A488"> Alexa Fluor 488 <span class="conjugate-indicator" style="background-color:#36FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/MA3-019-A555"> Alexa Fluor 555 <span class="conjugate-indicator" style="background-color:#E1FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/MA3-019-A647"> Alexa Fluor 647 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li class="drop-down-link-ctnr"> <a class="conjugate-dropdown-link" href="/us/en/home/global/forms/antibody-conjugates-custom-form.html" ng-attr-target="$ctrl.getRequestCustomLinkTarget()">Request custom conjugation <svg class="svg-icon external-link-icon" ng-if="$ctrl.showExternalLinkIcon()"> <use href="#external-link"></use> </svg> </a> </li> </ul> </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Form</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Liquid </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Concentration</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 1 mg/mL </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Purification</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Protein A </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage buffer</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> PBS with 1mg/mL BSA </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Contains</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.05% sodium azide </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> -20° C, Avoid Freeze/Thaw Cycles </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Shipping conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Wet ice </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"><h2>RRID <span id="rrid-tooltip" class="rrid-tooltip-icon" ng-include="'/order/genome-database/webapp/assets/core/img/svg/mono_tooltip.svg'" data-toggle="tooltip" ng-click="$ctrl.showRridTooltip()" close-tooltip="$ctrl.closeRridTooltip()" title="Research Resource Identifiers (#RRID) are ID numbers assigned to help researchers cite key resources in the biomedical literature to improve transparency of research methods."> </span></h2> </div> <div class="product-item left-flex-cls">AB_2163120</div> </div> </div> <div class="product-specific-details"> <div class="product-specific-details-tbl product-specific-add-border" > <div class="product-info-para"> <h4 class="product-info-para-heading"> Product Specific Information </h4> <p>MA3-019 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.<br /><br />MA3-019 has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-019 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-019 has been shown to inhibit the activity of PDI in vitro. MA3-019 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.<br /><br />In immunohistochemistry procedures, formalin-fixed paraffin-embedded tissue sections are recommended.<br /><br />The MA3-019 antigen is purified rat PDI.</p> </div> <div class="product-info-para"> <h4 class="product-info-para-heading"> Target Information </h4> <p class="product-specific-target-info">Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.</p> </div> <p class="product-details-regulatoryStmt">For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.</p> </div> <div class="product-detail-panel-builder"> <tf-promo-ad ng-if="$ctrl.showPromoAd" show-promo-ad="$ctrl.showPromoAd"></tf-promo-ad> </div> </div> </div> </div> </div> <div id="rootPdpDocLoader"></div> <div id="tf-related-products-container" ng-show="emptyRelatedProductsWidget === false" > <related-products-container ng-if="showRelatedProductsWidget === true" base-url="'/api/magellan/related-products-ui/widget/v2/'" product-type="'antibody'" product-sub-type="'primary'" product-id="'MA3019'" country-code="'sg'" iso-language="'en'" > </related-products-container> </div> <div class="tf-pdp-divider" ng-if="emptyRelatedProductsWidget === false" ></div> <div class="references reference-section-3" id="references-component-id"> <div id="references-section-id"> <reference-section ng-if="loadReferences" default-display-card-limit="3" reference-count="87" sub-type="'Antibody'" 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itemtype="https://schema.org/ScholarlyArticle"> <h2>Product References</h2> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36463755"> <div itemprop="name">A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes.</div> </a> <div itemprop="about">EBioMedicine</div> <div itemprop="author">Syed F,Singhal D,Raedschelders K,Krishnan P,Bone RN,McLaughlin MR,Van Eyk JE,Mirmira RG,Yang ML,Mamula MJ,Wu H,Liu X,Evans-Molina C</div> <div itemprop="description">MA3-019 was used in Competition assay to identify a core set of modulated pathways across distinct mouse models of T1D and identified PDIA1 as a potential human biomarker of β cell stress in T1D.</div> <div itemprodp="datePublished">Sun Jan 01 00:00:00 EST 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35466528"> <div itemprop="name">von Willebrand factor propeptide variants lead to impaired storage and ER retention in 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MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Fri Jul 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34816548"> <div itemprop="name">GGCX variants leading to biallelic deficiency to γ-carboxylate GRP cause skin laxity in VKCFD1 patients.</div> </a> <div itemprop="about">Human mutation</div> <div itemprop="author">Ghosh S,Kraus K,Biswas A,Müller J,Forin F,Singer H,Höning K,Hornung V,Watzka M,Oldenburg J,Czogalla-Nitsche KJ</div> <div itemprop="description">MA3-019 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Western Blot to understand the development of non-hemorrhagic phenotypes and hence improve treatment in VKCFD1 patients by genotype-phenotype analysis.</div> <div itemprodp="datePublished">Sat Jan 01 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35176859"> <div 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itemprodp="datePublished">Tue Aug 30 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34259830"> <div itemprop="name">Engrailed 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation.</div> </a> <div itemprop="about">The Journal of experimental medicine</div> <div itemprop="author">Györfi AH,Matei AE,Fuchs M,Liang C,Rigau AR,Hong X,Zhu H,Luber M,Bergmann C,Dees C,Ludolph I,Horch RE,Distler O,Wang J,Bengsch B,Schett G,Kunz M,Distler JHW</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunohistochemistry</div> <div itemprodp="datePublished">Mon Sep 06 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34259830"> <div itemprop="name">Engrailed 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation.</div> </a> <div itemprop="about">The Journal of experimental medicine</div> <div itemprop="author">Györfi 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itemprop="description">MA3-019 was used in Immunocytochemistry to propose that the norovirus NTPase may utilize LDs or the ER as replication platforms to benefit viral replication and pathogenesis.</div> <div itemprodp="datePublished">Fri Sep 03 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34431704"> <div itemprop="name">Identification and Characterization of Human Norovirus NTPase Regions Required for Lipid Droplet Localization, Cellular Apoptosis, and Interaction with the Viral P22 Protein.</div> </a> <div itemprop="about">Microbiology spectrum</div> <div itemprop="author">Yen JB,Chen LW,Wei LH,Hung CH,Wang SS,Lin CL,Chang PJ</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry to propose that the norovirus NTPase may utilize LDs or the ER as replication platforms to benefit viral replication and pathogenesis.</div> <div itemprodp="datePublished">Fri Sep 03 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33572517"> <div itemprop="name">Potential Dual Role of West Nile Virus NS2B in Orchestrating NS3 Enzymatic Activity in Viral Replication.</div> </a> <div itemprop="about">Viruses</div> <div itemprop="author">Tseng AC,Nerurkar VR,Neupane KR,Kae H,Kaufusi PH</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Sun Jan 31 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34331747"> <div itemprop="name">The c.863A>G (p.Glu288Gly) variant of the CTSD gene is not associated with CLN10 disease.</div> </a> <div itemprop="about">Molecular genetics & genomic medicine</div> <div itemprop="author">Yang J,Ding X,Meng S,Cai J,Zhou W</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Fri Oct 01 00:00:00 EDT 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itemprop="description">MA3-019 was used in Immunohistochemistry to identify a pathway of epigenetic imprinting of fibroblasts in fibrotic disease with translational implications for the development of targeted therapies in fibrotic diseases.</div> <div itemprodp="datePublished">Fri May 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31634494"> <div itemprop="name">Influences of cyclosporin A and non-immunosuppressive derivatives on cellular cyclophilins and viral nucleocapsid protein during human coronavirus 229E replication.</div> </a> <div itemprop="about">Antiviral research</div> <div itemprop="author">Ma-Lauer Y,Zheng Y,Malešević M,von Brunn B,Fischer G,von Brunn A</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Wed Jan 01 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31634494"> <div itemprop="name">Influences of cyclosporin A and non-immunosuppressive derivatives on cellular cyclophilins and viral nucleocapsid protein during human coronavirus 229E replication.</div> </a> <div itemprop="about">Antiviral research</div> <div itemprop="author">Ma-Lauer Y,Zheng Y,Malešević M,von Brunn B,Fischer G,von Brunn A</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Wed Jan 01 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32528962"> <div itemprop="name">Overexpression of FAM46A, a Non-canonical Poly(A) Polymerase, Promotes Hemin-Induced Hemoglobinization in K562 Cells.</div> </a> <div itemprop="about">Frontiers in cell and developmental biology</div> <div itemprop="author">Lin HH,Lo YL,Wang WC,Huang KY,I KY,Chang GW</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to summarize that FAM46A is a novel poly(A) polymerase that functions as a critical intracellular modulator of hemoglobinization.</div> <div itemprodp="datePublished">Mon Sep 28 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32528962"> <div itemprop="name">Overexpression of FAM46A, a Non-canonical Poly(A) Polymerase, Promotes Hemin-Induced Hemoglobinization in K562 Cells.</div> </a> <div itemprop="about">Frontiers in cell and developmental biology</div> <div itemprop="author">Lin HH,Lo YL,Wang WC,Huang KY,I KY,Chang GW</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to summarize that FAM46A is a novel poly(A) polymerase that functions as a critical intracellular modulator of hemoglobinization.</div> <div itemprodp="datePublished">Mon Sep 28 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32294302"> <div itemprop="name">Novel role of dynamin-related-protein 1 in dynamics of ER-lipid droplets in adipose tissue.</div> </a> <div itemprop="about">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</div> <div itemprop="author">Li X,Yang L,Mao Z,Pan X,Zhao Y,Gu X,Eckel-Mahan K,Zuo Z,Tong Q,Hartig SM,Cheng X,Du G,Moore DD,Bellen HJ,Sesaki H,Sun K</div> <div itemprop="description">MA3-019 was used in Western Blotting to report that DRP1 translocates to endoplasmic reticulum (ER) in response to β-adrenergic stimulation.</div> <div itemprodp="datePublished">Mon Jun 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33270690"> <div itemprop="name">Shear stress-exposed pulmonary artery endothelial cells fail to upregulate HSP70 in chronic thromboembolic pulmonary hypertension.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Salibe-Filho W,Araujo TLS,G Melo E,B C T Coimbra L,Lapa MS,Acencio MMP,Freitas-Filho O,Capelozzi VL,Teixeira LR,Fernandes CJCS,Jatene FB,Laurindo FRM,Terra-Filho M</div> <div itemprop="description">MA3-019 was used in Western Blotting to investigate the pathophysiological mechanisms underlying chronic thromboembolic pulmonary hypertension.</div> <div itemprodp="datePublished">Mon Jan 18 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33270690"> <div itemprop="name">Shear stress-exposed pulmonary artery endothelial cells fail to upregulate HSP70 in chronic thromboembolic pulmonary hypertension.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Salibe-Filho W,Araujo TLS,G Melo E,B C T Coimbra L,Lapa MS,Acencio MMP,Freitas-Filho O,Capelozzi VL,Teixeira LR,Fernandes CJCS,Jatene FB,Laurindo FRM,Terra-Filho M</div> <div itemprop="description">MA3-019 was used in Western Blotting to investigate the pathophysiological mechanisms underlying chronic thromboembolic pulmonary hypertension.</div> <div itemprodp="datePublished">Mon Jan 18 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32924931"> <div itemprop="name">TMEM79/MATTRIN defines a pathway for Frizzled regulation and is required for <i>Xenopus embryogenesis</i>.</div> </a> <div itemprop="about">eLife</div> <div itemprop="author">Chen M,Amado N,Tan J,Reis A,Ge M,Abreu JG,He X</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to identify, via CRISPR/Cas9 genome-wide loss-of function screening, an orphan five-pass transmembrane protein TMEM79 (also called MATTRIN), as a specific antagonist of Wnt signaling.</div> <div itemprodp="datePublished">Mon Sep 14 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30710055"> <div itemprop="name">Heterotypic CAF-tumor spheroids promote early peritoneal metastatis of ovarian cancer.</div> </a> <div itemprop="about">The Journal of experimental medicine</div> <div itemprop="author">Gao Q,Yang Z,Xu S,Li X,Yang X,Jin P,Liu Y,Zhou X,Zhang T,Gong C,Wei X,Liu D,Sun C,Chen G,Hu J,Meng L,Zhou J,Sawada K,Fruscio R,Grunt TW,Wischhusen J,Vargas-Hernández VM,Pothuri B,Coleman RL</div> <div itemprop="description">MA3-019 was used in Immunofluorescence in immunohistochemistry on frozen tissues to study the aggressive nature of high-grade serous ovarian cancer ascitic tumour cells, which are prone to forming heterotypic spheroids with fibroblasts.</div> <div itemprodp="datePublished">Mon Mar 04 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30209302"> <div itemprop="name">Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER-mitochondria communication during the early phase of ER stress.</div> </a> <div itemprop="about">Cell death and differentiation</div> <div itemprop="author">Bravo-Sagua R,Parra V,Ortiz-Sandoval C,Navarro-Marquez M,Rodríguez AE,Diaz-Valdivia N,Sanhueza C,Lopez-Crisosto C,Tahbaz N,Rothermel BA,Hill JA,Cifuentes M,Simmen T,Quest AFG,Lavandero S</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</div> <div itemprodp="datePublished">Mon Jul 01 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30580571"> <div itemprop="name">Redox Activation of Nox1 (NADPH Oxidase 1) Involves an Intermolecular Disulfide Bond Between Protein Disulfide Isomerase and p47<sup>phox</sup> in Vascular Smooth Muscle Cells.</div> </a> <div itemprop="about">Arteriosclerosis, thrombosis, and vascular biology</div> <div itemprop="author">Gimenez M,Veríssimo-Filho S,Wittig I,Schickling BM,Hahner F,Schürmann C,Netto LES,Rosa JC,Brandes RP,Sartoretto S,De Lucca Camargo L,Abdulkader F,Miller FJ,Lopes LR</div> <div itemprop="description">MA3-019 was used in Immunohistochemistry to investigate the molecular mechanism by which PDI (protein disulfide isomerase A1) activates Nox1 (NADPH oxidase) and the functional implications of PDI in Nox1 activation in vascular disease.</div> <div itemprodp="datePublished">Fri Feb 01 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30645756"> <div itemprop="name">Cell-surface HSP70 associates with thrombomodulin in endothelial cells.</div> </a> <div itemprop="about">Cell stress & chaperones</div> <div itemprop="author">Araujo TLS,Venturini G,Moretti AIS,Tanaka LY,Pereira AC,Laurindo FRM</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</div> <div itemprodp="datePublished">Tue Jan 01 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30760703"> <div itemprop="name">Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas.</div> </a> <div itemprop="about">Cell death & disease</div> <div itemprop="author">De Bessa TC,Pagano A,Moretti AIS,Oliveira PVS,Mendonça SA,Kovacic H,Laurindo FRM</div> <div itemprop="description">MA3-019 was used in Western Blotting to examine the mechanisms by which protein disulphide isomerases, including PDIA1, are implicated in cancer progression.</div> <div itemprodp="datePublished">Wed Feb 13 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30760703"> <div itemprop="name">Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas.</div> </a> <div itemprop="about">Cell death & disease</div> <div itemprop="author">De Bessa TC,Pagano A,Moretti AIS,Oliveira PVS,Mendonça SA,Kovacic H,Laurindo FRM</div> <div itemprop="description">MA3-019 was used in Western Blotting to examine the mechanisms by which protein disulphide isomerases, including PDIA1, are implicated in cancer progression.</div> <div itemprodp="datePublished">Wed Feb 13 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30967547"> <div itemprop="name">Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Alenquer M,Vale-Costa S,Etibor TA,Ferreira F,Sousa AL,Amorim MJ</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Tue Apr 09 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30827871"> <div itemprop="name">Alzheimer Aβ Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKα3 Neurons by Secretion.</div> </a> <div itemprop="about">iScience</div> <div itemprop="author">Komura H,Kakio S,Sasahara T,Arai Y,Takino N,Sato M,Satomura K,Ohnishi T,Nabeshima YI,Muramatsu SI,Kii I,Hoshi M</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry to study the location in neurons where amylospheroids form and how they reach their targets to induce neurodegeneration.</div> <div itemprodp="datePublished">Fri Mar 29 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29212938"> <div itemprop="name">Subcellular Localization and Functional Characterization of GII.4 Norovirus-Encoded NTPase.</div> </a> <div itemprop="about">Journal of virology</div> <div itemprop="author">Yen JB,Wei LH,Chen LW,Chen LY,Hung CH,Wang SS,Chang PJ</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to show that the virus-encoded NTPase must have multiple activities during viral replication or pathogenesis.</div> <div itemprodp="datePublished">Thu Mar 01 00:00:00 EST 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29581535"> <div itemprop="name">Mitochondrial fragmentation affects neither the sensitivity to TNFα-induced apoptosis of Brucella-infected cells nor the intracellular replication of the bacteria.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Lobet E,Willemart K,Ninane N,Demazy C,Sedzicki J,Lelubre C,De Bolle X,Renard P,Raes M,Dehio C,Letesson JJ,Arnould T</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Mon Mar 26 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30139741"> <div itemprop="name">StAR-related lipid transfer domain 11 (STARD11)-mediated ceramide transport mediates extracellular vesicle biogenesis.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Fukushima M,Dasgupta D,Mauer AS,Kakazu E,Nakao K,Malhi H</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to propose a model of how StAR-related lipid transfer domain 11 mediates ceramide trafficking in palmitate-treated cells and stimulates exosome biogenesis.</div> <div itemprodp="datePublished">Fri Sep 28 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30139741"> <div itemprop="name">StAR-related lipid transfer domain 11 (STARD11)-mediated ceramide transport mediates extracellular vesicle biogenesis.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Fukushima M,Dasgupta D,Mauer AS,Kakazu E,Nakao K,Malhi H</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to propose a model of how StAR-related lipid transfer domain 11 mediates ceramide trafficking in palmitate-treated cells and stimulates exosome biogenesis.</div> <div itemprodp="datePublished">Fri Sep 28 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30555302"> <div itemprop="name">Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway.</div> </a> <div itemprop="about">Frontiers in cellular neuroscience</div> <div itemprop="author">Lambert L,Dubayle D,Fafouri A,Herzog E,Csaba Z,Dournaud P,El Mestikawy S,Bernard V</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Tue Sep 29 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30555302"> <div itemprop="name">Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway.</div> </a> <div itemprop="about">Frontiers in cellular neuroscience</div> <div itemprop="author">Lambert L,Dubayle D,Fafouri A,Herzog E,Csaba Z,Dournaud P,El Mestikawy S,Bernard V</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Tue Sep 29 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29484832"> <div itemprop="name">Endoplasmic reticulum, Golgi, and lysosomes are disorganized in lung fibroblasts from chronic obstructive pulmonary disease patients.</div> </a> <div itemprop="about">Physiological reports</div> <div itemprop="author">Weidner J,Jarenbäck L,Åberg I,Westergren-Thorsson G,Ankerst J,Bjermer L,Tufvesson E</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to show that COPD patients have phenotypic changes in the lung fibroblasts endomembrane pathway, and respond differently to stress.</div> <div itemprodp="datePublished">Thu Mar 01 00:00:00 EST 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29305423"> <div itemprop="name">Endoplasmic reticulum-resident protein 57 (ERp57) oxidatively inactivates human transglutaminase 2.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Yi MC,Melkonian AV,Ousey JA,Khosla C</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to investigate switch 'off' mechanism of transglutaminase 2 (TG2).</div> <div itemprodp="datePublished">Fri Feb 23 00:00:00 EST 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29083321"> <div itemprop="name">EMC3 coordinates surfactant protein and lipid homeostasis required for respiration.</div> </a> <div itemprop="about">The Journal of clinical investigation</div> <div itemprop="author">Tang X,Snowball JM,Xu Y,Na CL,Weaver TE,Clair G,Kyle JE,Zink EM,Ansong C,Wei W,Huang M,Lin X,Whitsett JA</div> <div itemprop="description">MA3-019 was used in Immunohistochemistry to demonstrate that EMC3 is required for murine pulmonary surfactant synthesis and lung function at birth.</div> <div itemprodp="datePublished">Fri Dec 01 00:00:00 EST 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28951536"> <div itemprop="name">Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.</div> </a> <div itemprop="about">Science signaling</div> <div itemprop="author">Waters AM,Ozkan-Dagliyan I,Vaseva AV,Fer N,Strathern LA,Hobbs GA,Tessier-Cloutier B,Gillette WK,Bagni R,Whiteley GR,Hartley JL,McCormick F,Cox AD,Houghton PJ,Huntsman DG,Philips MR,Der CJ</div> <div itemprop="description">MA3-019 was used in Immunocytochemistry-immunoflourescence to evaluate 22 commercially available anti-RAS antibodies with a set of distinct reagents and cell lines for their specificity and selectivity in recognising the intended RAS isoforms and mutants.</div> <div itemprodp="datePublished">Tue Sep 26 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28376661"> <div itemprop="name">Succination of Protein Disulfide Isomerase Links Mitochondrial Stress and Endoplasmic Reticulum Stress in the Adipocyte During Diabetes.</div> </a> <div itemprop="about">Antioxidants & redox signaling</div> <div itemprop="author">Manuel AM,Walla MD,Faccenda A,Martin SL,Tanis RM,Piroli GG,Adam J,Kantor B,Mutus B,Townsend DM,Frizzell N</div> <div itemprop="description">MA3-019 was used in Western Blotting to investigate whether succination alters PDI oxidoreductase activity, directly linking mitochondrial stress and endoplasmic reticulum stress.</div> <div itemprodp="datePublished">Fri Dec 01 00:00:00 EST 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28376661"> <div itemprop="name">Succination of Protein Disulfide Isomerase Links Mitochondrial Stress and Endoplasmic Reticulum Stress in the Adipocyte During Diabetes.</div> </a> <div itemprop="about">Antioxidants & redox signaling</div> <div itemprop="author">Manuel AM,Walla MD,Faccenda A,Martin SL,Tanis RM,Piroli GG,Adam J,Kantor B,Mutus B,Townsend DM,Frizzell N</div> <div itemprop="description">MA3-019 was used in Western Blotting to investigate whether succination alters PDI oxidoreductase activity, directly linking mitochondrial stress and endoplasmic reticulum stress.</div> <div itemprodp="datePublished">Fri Dec 01 00:00:00 EST 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27381823"> <div itemprop="name">Embryonic lethal abnormal vision proteins and adenine and uridine-rich element mRNAs after global cerebral ischemia and reperfusion in the rat.</div> </a> <div itemprop="about">Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism</div> <div itemprop="author">Wang H,Tri Anggraini F,Chen X,DeGracia DJ</div> <div itemprop="description">MA3019 was used in western blot to study HuR, HuB, HuC, and HuD in hippocampal CA1 neurons</div> <div itemprodp="datePublished">Sat Apr 01 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28628197"> <div itemprop="name">Vesicular acetylcholine transporter (VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function.</div> </a> <div itemprop="about">Journal of neurochemistry</div> <div itemprop="author">Janickova H,Prado VF,Prado MAM,El Mestikawy S,Bernard V</div> <div itemprop="description">MA3-019 was used in Immunohistochemistry to demonstrate that ChAT-ChR2 mice present major alterations of CIN neuronal morphology and increased behavioral sensitization to cocaine, supporting the notion that the increased levels of VAChT observed in these mice make them fundamentally different from wild-type mice.</div> <div itemprodp="datePublished">Fri Sep 01 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29222525"> <div itemprop="name">Conserved Gene Microsynteny Unveils Functional Interaction Between Protein Disulfide Isomerase and Rho Guanine-Dissociation Inhibitor Families.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Moretti AIS,Pavanelli JC,Nolasco P,Leisegang MS,Tanaka LY,Fernandes CG,Wosniak J,Kajihara D,Dias MH,Fernandes DC,Jo H,Tran NV,Ebersberger I,Brandes RP,Bonatto D,Laurindo FRM</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Fri Dec 08 00:00:00 EST 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28489076"> <div itemprop="name">HLA-DP<sup>84Gly</sup> constitutively presents endogenous peptides generated by the class I antigen processing pathway.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Yamashita Y,Anczurowski M,Nakatsugawa M,Tanaka M,Kagoya Y,Sinha A,Chamoto K,Ochi T,Guo T,Saso K,Butler MO,Minden MD,Kislinger T,Hirano N</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Wed May 10 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26781284"> <div itemprop="name">Peri/Epicellular Protein Disulfide Isomerase Sustains Vascular Lumen Caliber Through an Anticonstrictive Remodeling Effect.</div> </a> <div itemprop="about">Hypertension (Dallas, Tex. : 1979)</div> <div itemprop="author">Tanaka LY,Araújo HA,Hironaka GK,Araujo TL,Takimura CK,Rodriguez AI,Casagrande AS,Gutierrez PS,Lemos-Neto PA,Laurindo FR</div> <div itemprop="description">MA3-019 was used in Neutralization experiments to study the role of the cell surface pool of the endoplasmic reticulum redox chaperone protein disulfide isomerase-A1 on the regulation of disease associated vascular architecture.</div> <div itemprodp="datePublished">Tue Mar 01 00:00:00 EST 2016</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26879174"> <div itemprop="name">An ERp57-mediated disulphide exchange promotes the interaction between Burkholderia cenocepacia and epithelial respiratory cells.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Pacello F,D'Orazio M,Battistoni A</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to identify ERp57 as a protein disulfide isomerase involved in the interaction between Burkholderia cenocepacia and epithelial cells</div> <div itemprodp="datePublished">Tue Feb 16 00:00:00 EST 2016</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/25561173"> <div itemprop="name">Optogenetic control of organelle transport and positioning.</div> </a> <div itemprop="about">Nature</div> <div itemprop="author">van Bergeijk P,Adrian M,Hoogenraad CC,Kapitein LC</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to establish a method of optical control of intracellular transport using light-sensitive heterodimerization to recruit specific cytoskeletal motor proteins to selected cargoes and test how local positioning of recycling endosomes contributes to axon</div> <div itemprodp="datePublished">Thu Feb 05 00:00:00 EST 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26551543"> <div itemprop="name">Optogenetic acidification of synaptic vesicles and lysosomes.</div> </a> <div itemprop="about">Nature neuroscience</div> <div itemprop="author">Rost BR,Schneider F,Grauel MK,Wozny C,Bentz C,Blessing A,Rosenmund T,Jentsch TJ,Schmitz D,Hegemann P,Rosenmund C</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to analyze synaptic vesicles and lysosomes and optogenetic acidification</div> <div itemprodp="datePublished">Tue Dec 01 00:00:00 EST 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/25852189"> <div itemprop="name">An analysis of critical factors for quantitative immunoblotting.</div> </a> <div itemprop="about">Science signaling</div> <div itemprop="author">Janes KA</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</div> <div itemprodp="datePublished">Tue Apr 07 00:00:00 EDT 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26089538"> <div itemprop="name">Biogenesis and cytotoxicity of APOL1 renal risk variant proteins in hepatocytes and hepatoma cells.</div> </a> <div itemprop="about">Journal of lipid research</div> <div itemprop="author">Cheng D,Weckerle A,Yu Y,Ma L,Zhu X,Murea M,Freedman BI,Parks JS,Shelness GS</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Sat Aug 01 00:00:00 EDT 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26089538"> <div itemprop="name">Biogenesis and cytotoxicity of APOL1 renal risk variant proteins in hepatocytes and hepatoma cells.</div> </a> <div itemprop="about">Journal of lipid research</div> <div itemprop="author">Cheng D,Weckerle A,Yu Y,Ma L,Zhu X,Murea M,Freedman BI,Parks JS,Shelness GS</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Sat Aug 01 00:00:00 EDT 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/24361341"> <div itemprop="name">Characterization of N-glycosylation sites on the extracellular domain of NOX1/NADPH oxidase.</div> </a> <div itemprop="about">Free radical biology & medicine</div> <div itemprop="author">Matsumoto M,Katsuyama M,Iwata K,Ibi M,Zhang J,Zhu K,Nauseef WM,Yabe-Nishimura C</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to identify N-glycosylation sites on the NOX1 subunit of NADPH oxidase</div> <div itemprodp="datePublished">Sat Mar 01 00:00:00 EST 2014</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/25135935"> <div itemprop="name">Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.</div> </a> <div itemprop="about">The Journal of cell biology</div> <div itemprop="author">Cherepanova NA,Shrimal S,Gilmore R</div> <div itemprop="description">MA3-019 was used in western blot to determine how N-glycosylation of cysteine-proximal acceptor sites in glycoproteins requires oxidoreductase activity</div> <div itemprodp="datePublished">Mon Aug 18 00:00:00 EDT 2014</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/24406246"> <div itemprop="name">Transport of estradiol-17β-glucuronide, estrone-3-sulfate and taurocholate across the endoplasmic reticulum membrane: evidence for different transport systems.</div> </a> <div itemprop="about">Biochemical pharmacology</div> <div itemprop="author">Wlcek K,Hofstetter L,Stieger B</div> <div itemprop="description">MA3-019 was used in western blot to study the involvement of different systems in the ER transport of estradiol-17beta-glucuronide, estrone-3-sulfate and taurocholate</div> <div itemprodp="datePublished">Sat Mar 01 00:00:00 EST 2014</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/24788773"> <div itemprop="name">Effects of oxidative stress on the solubility of HRD1, a ubiquitin ligase implicated in Alzheimer's disease.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Saito R,Kaneko M,Kitamura Y,Takata K,Kawada K,Okuma Y,Nomura Y</div> <div itemprop="description">MA3-019 was used in western blot to study the effects of oxidative stress on the solubility of the Alzheimer's disease-associated E3 ubiquitin ligase HRD1</div> <div itemprodp="datePublished">Thu Dec 18 00:00:00 EST 2014</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/24323415"> <div itemprop="name">mRNA redistribution during permanent focal cerebral ischemia.</div> </a> <div itemprop="about">Translational stroke research</div> <div itemprop="author">Lewis MK,Jamison JT,Dunbar JC,DeGracia DJ</div> <div itemprop="description">MA3-019 was used in immunohistochemistry to study mRNA granule formation following experimental permanent focal cerebral ischemia</div> <div itemprodp="datePublished">Sun Dec 01 00:00:00 EST 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23344956"> <div itemprop="name">Depletion of molecular chaperones from the endoplasmic reticulum and fragmentation of the Golgi apparatus associated with pathogenesis in Pelizaeus-Merzbacher disease.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Numata Y,Morimura T,Nakamura S,Hirano E,Kure S,Goto YI,Inoue K</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Fri Mar 15 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23344956"> <div itemprop="name">Depletion of molecular chaperones from the endoplasmic reticulum and fragmentation of the Golgi apparatus associated with pathogenesis in Pelizaeus-Merzbacher disease.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Numata Y,Morimura T,Nakamura S,Hirano E,Kure S,Goto YI,Inoue K</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Fri Mar 15 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23344956"> <div itemprop="name">Depletion of molecular chaperones from the endoplasmic reticulum and fragmentation of the Golgi apparatus associated with pathogenesis in Pelizaeus-Merzbacher disease.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Numata Y,Morimura T,Nakamura S,Hirano E,Kure S,Goto YI,Inoue K</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Fri Mar 15 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23344956"> <div itemprop="name">Depletion of molecular chaperones from the endoplasmic reticulum and fragmentation of the Golgi apparatus associated with pathogenesis in Pelizaeus-Merzbacher disease.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Numata Y,Morimura T,Nakamura S,Hirano E,Kure S,Goto YI,Inoue K</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunocytochemistry</div> <div itemprodp="datePublished">Fri Mar 15 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22146474"> <div itemprop="name">Unraveling the human dendritic cell phagosome proteome by organellar enrichment ranking.</div> </a> <div itemprop="about">Journal of proteomics</div> <div itemprop="author">Buschow SI,Lasonder E,Szklarczyk R,Oud MM,de Vries IJ,Figdor CG</div> <div itemprop="description">MA3-019 was used in western blot to study the protein composition of phagosomes from human dendritic cell phagosome</div> <div itemprodp="datePublished">Thu Feb 16 00:00:00 EST 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22398681"> <div itemprop="name">Inhibiting rotavirus infection by membrane-impermeant thiol/disulfide exchange blockers and antibodies against protein disulfide isomerase.</div> </a> <div itemprop="about">Intervirology</div> <div itemprop="author">Calderon MN,Guerrero CA,Acosta O,Lopez S,Arias CF</div> <div itemprop="description">MA3-019 was used in flow cytometry to study the ability of non-cell-penetrating thiol-disulphide exchange inhibitors to reduce rotavirus infectivity and the potential involvement of protein disulfide isomerase</div> <div itemprodp="datePublished">Mon Apr 08 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21978884"> <div itemprop="name">Organelles do not colocalize with mRNA granules in post-ischemic neurons.</div> </a> <div itemprop="about">Neuroscience</div> <div itemprop="author">Jamison JT,Szymanski JJ,Degracia DJ</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Western Blot</div> <div itemprodp="datePublished">Thu Dec 29 00:00:00 EST 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22025618"> <div itemprop="name">NLRP7, a nucleotide oligomerization domain-like receptor protein, is required for normal cytokine secretion and co-localizes with Golgi and the microtubule-organizing center.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Messaed C,Akoury E,Djuric U,Zeng J,Saleh M,Gilbert L,Seoud M,Qureshi S,Slim R</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to investigate the role of NLRP7 in normal cytokine secretion and its colocalization with Golgi</div> <div itemprodp="datePublished">Fri Dec 16 00:00:00 EST 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22025618"> <div itemprop="name">NLRP7, a nucleotide oligomerization domain-like receptor protein, is required for normal cytokine secretion and co-localizes with Golgi and the microtubule-organizing center.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Messaed C,Akoury E,Djuric U,Zeng J,Saleh M,Gilbert L,Seoud M,Qureshi S,Slim R</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to investigate the role of NLRP7 in normal cytokine secretion and its colocalization with Golgi</div> <div itemprodp="datePublished">Fri Dec 16 00:00:00 EST 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21253612"> <div itemprop="name">Elimination of hepatitis C virus from hepatocytes by a selective activation of therapeutic molecules.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Wen X,Abe T,Kukihara H,Taguwa S,Mori Y,Tani H,Kato N,Suzuki T,Tatsumi M,Moriishi K,Matsuura Y</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to evaluate the efficacy of gene transfer therapy in clearing hepatitis C virus from hepatocytes</div> <div itemprodp="datePublished">Thu Jan 06 00:00:00 EST 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20237263"> <div itemprop="name">Loss of HRD1-mediated protein degradation causes amyloid precursor protein accumulation and amyloid-beta generation.</div> </a> <div itemprop="about">The Journal of neuroscience : the official journal of the Society for Neuroscience</div> <div itemprop="author">Kaneko M,Koike H,Saito R,Kitamura Y,Okuma Y,Nomura Y</div> <div itemprop="description">MA3-019 was used in western blot to study role of protein degradation on endoplasmic reticulum stress and Alzheimer plaque deposits</div> <div itemprodp="datePublished">Wed Mar 17 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20453113"> <div itemprop="name">mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum.</div> </a> <div itemprop="about">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</div> <div itemprop="author">Unsworth H,Raguz S,Edwards HJ,Higgins CF,Yagüe E</div> <div itemprop="description">MA3-019 was used in western blot to investigate the process of mRNA translation during stress response</div> <div itemprodp="datePublished">Wed Sep 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20638985"> <div itemprop="name">Characterization of the expression, localization, and secretion of PANDER in alpha-cells.</div> </a> <div itemprop="about">Molecular and cellular endocrinology</div> <div itemprop="author">Carnegie JR,Robert-Cooperman CE,Wu J,Young RA,Wolf BA,Burkhardt BR</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to investigate the properties of PANDER in alpha-cells</div> <div itemprodp="datePublished">Mon Aug 30 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20638985"> <div itemprop="name">Characterization of the expression, localization, and secretion of PANDER in alpha-cells.</div> </a> <div itemprop="about">Molecular and cellular endocrinology</div> <div itemprop="author">Carnegie JR,Robert-Cooperman CE,Wu J,Young RA,Wolf BA,Burkhardt BR</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to investigate the properties of PANDER in alpha-cells</div> <div itemprodp="datePublished">Mon Aug 30 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20546900"> <div itemprop="name">DC-STAMP interacts with ER-resident transcription factor LUMAN which becomes activated during DC maturation.</div> </a> <div itemprop="about">Molecular immunology</div> <div itemprop="author">Eleveld-Trancikova D,Sanecka A,van Hout-Kuijer MA,Looman MW,Hendriks IA,Jansen BJ,Adema GJ</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to study the interaction between DC-STAMP interacts and estrogen receptor-resident transcription factor LUMAN</div> <div itemprodp="datePublished">Thu Jul 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20848131"> <div itemprop="name">Mobile ER-to-Golgi but not post-Golgi membrane transport carriers disappear during the terminal myogenic differentiation.</div> </a> <div itemprop="about">Cell and tissue research</div> <div itemprop="author">Nevalainen M,Kaisto T,Metsikkö K</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to investigate the difference of ER-to-Golgi trafficking between adult skeletal myofibers and other mammalian cell types</div> <div itemprodp="datePublished">Fri Oct 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19211270"> <div itemprop="name">Biomechanical stress induces novel arterial intima-enriched genes: implications for vascular adaptation to stress.</div> </a> <div itemprop="about">Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology</div> <div itemprop="author">Pyle AL,Li B,Maupin AB,Guzman RJ,Crimmins DL,Olson S,Atkinson JB,Young PP</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to study the role of biomechanical stress in vascular pathologies</div> <div itemprodp="datePublished">Tue May 04 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20537980"> <div itemprop="name">Membrane topology of human AGPAT3 (LPAAT3).</div> </a> <div itemprop="about">Biochemical and biophysical research communications</div> <div itemprop="author">Schmidt JA,Yvone GM,Brown WJ</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to investigate the structural arrangement of AGPAT3 in membrane and its significance in the enzymatic activity</div> <div itemprodp="datePublished">Fri Jul 09 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19760141"> <div itemprop="name">Regulation of ER molecular chaperone prevents bone loss in a murine model for osteoporosis.</div> </a> <div itemprop="about">Journal of bone and mineral metabolism</div> <div itemprop="author">Hino S,Kondo S,Yoshinaga K,Saito A,Murakami T,Kanemoto S,Sekiya H,Chihara K,Aikawa Y,Hara H,Kudo T,Sekimoto T,Funamoto T,Chosa E,Imaizumi K</div> <div itemprop="description">MA3-019 was used in immunohistochemistry to investigate the effect of endoplasmic reticulum stress on bone balance in a mouse osteoporosis model</div> <div itemprodp="datePublished">Mon Mar 01 00:00:00 EST 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20667829"> <div itemprop="name">Production of anti-carbohydrate antibodies by phage display technologies: potential impairment of cell growth as a result of endogenous expression.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Yuasa N,Zhang W,Goto T,Sakaue H,Matsumoto-Takasaki A,Kimura M,Ohshima H,Tsuchida Y,Koizumi T,Sakai K,Kojima T,Yamamoto K,Nakata M,Fujita-Yamaguchi Y</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Fri Oct 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20667829"> <div itemprop="name">Production of anti-carbohydrate antibodies by phage display technologies: potential impairment of cell growth as a result of endogenous expression.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Yuasa N,Zhang W,Goto T,Sakaue H,Matsumoto-Takasaki A,Kimura M,Ohshima H,Tsuchida Y,Koizumi T,Sakai K,Kojima T,Yamamoto K,Nakata M,Fujita-Yamaguchi Y</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Immunofluorescence</div> <div itemprodp="datePublished">Fri Oct 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19192245"> <div itemprop="name">Calsyntenins mediate TGN exit of APP in a kinesin-1-dependent manner.</div> </a> <div itemprop="about">Traffic (Copenhagen, Denmark)</div> <div itemprop="author">Ludwig A,Blume J,Diep TM,Yuan J,Mateos JM,Leuthäuser K,Steuble M,Streit P,Sonderegger P</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to study the role kinesisn-1 in the calsyntenin-mediated trans-Golgi network exit of amyloid precursor protein</div> <div itemprodp="datePublished">Fri May 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19564574"> <div itemprop="name">Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages.</div> </a> <div itemprop="about">Journal of leukocyte biology</div> <div itemprop="author">Santos CX,Stolf BS,Takemoto PV,Amanso AM,Lopes LR,Souza EB,Goto H,Laurindo FR</div> <div itemprop="description">MA3-019 was used in immunoprecipitation to investigate the role of protein disulfide isomerase (PDI) during phagocytosis of Leishmania chagasi promastigotes by macrophages</div> <div itemprodp="datePublished">Thu Oct 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19564574"> <div itemprop="name">Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages.</div> </a> <div itemprop="about">Journal of leukocyte biology</div> <div itemprop="author">Santos CX,Stolf BS,Takemoto PV,Amanso AM,Lopes LR,Souza EB,Goto H,Laurindo FR</div> <div itemprop="description">MA3-019 was used in immunoprecipitation to investigate the role of protein disulfide isomerase (PDI) during phagocytosis of Leishmania chagasi promastigotes by macrophages</div> <div itemprodp="datePublished">Thu Oct 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19564574"> <div itemprop="name">Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages.</div> </a> <div itemprop="about">Journal of leukocyte biology</div> <div itemprop="author">Santos CX,Stolf BS,Takemoto PV,Amanso AM,Lopes LR,Souza EB,Goto H,Laurindo FR</div> <div itemprop="description">MA3-019 was used in immunoprecipitation to investigate the role of protein disulfide isomerase (PDI) during phagocytosis of Leishmania chagasi promastigotes by macrophages</div> <div itemprodp="datePublished">Thu Oct 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18434512"> <div itemprop="name">Dynamics of somatostatin type 2A receptor cargoes in living hippocampal neurons.</div> </a> <div itemprop="about">The Journal of neuroscience : the official journal of the Society for Neuroscience</div> <div itemprop="author">Lelouvier B,Tamagno G,Kaindl AM,Roland A,Lelievre V,Le Verche V,Loudes C,Gressens P,Faivre-Baumann A,Lenkei Z,Dournaud P</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to investigate the intracellular trafficking of somatostatin type 2A receptor cargoes in living hippocampal neurons.</div> <div itemprodp="datePublished">Wed Apr 23 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18331351"> <div itemprop="name">Interaction and functional association of protein disulfide isomerase with alphaVbeta3 integrin on endothelial cells.</div> </a> <div itemprop="about">The FEBS journal</div> <div itemprop="author">Swiatkowska M,Szymański J,Padula G,Cierniewski CS</div> <div itemprop="description">Published figure using PDI monoclonal antibody (Product # MA3-019) in Neutralization</div> <div itemprodp="datePublished">Tue Apr 01 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18426796"> <div itemprop="name">Endoplasmic reticulum (ER) chaperone regulation and survival of cells compensating for deficiency in the ER stress response kinase, PERK.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Yamaguchi Y,Larkin D,Lara-Lemus R,Ramos-Castañeda J,Liu M,Arvan P</div> <div itemprop="description">MA3-019 was used in western blot to study the relationship between endoplasmic reticulum chaperone regulation and the endoplasmic reticulum stress response kinase PERK.</div> <div itemprodp="datePublished">Fri Jun 20 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18426796"> <div itemprop="name">Endoplasmic reticulum (ER) chaperone regulation and survival of cells compensating for deficiency in the ER stress response kinase, PERK.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Yamaguchi Y,Larkin D,Lara-Lemus R,Ramos-Castañeda J,Liu M,Arvan P</div> <div itemprop="description">MA3-019 was used in western blot to study the relationship between endoplasmic reticulum chaperone regulation and the endoplasmic reticulum stress response kinase PERK.</div> <div itemprodp="datePublished">Fri Jun 20 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18056057"> <div itemprop="name">Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.</div> </a> <div itemprop="about">Molecular & cellular proteomics : MCP</div> <div itemprop="author">Gramolini AO,Kislinger T,Alikhani-Koopaei R,Fong V,Thompson NJ,Isserlin R,Sharma P,Oudit GY,Trivieri MG,Fagan A,Kannan A,Higgins DG,Huedig H,Hess G,Arab S,Seidman JG,Seidman CE,Frey B,Perry M,Backx PH,Liu PP,MacLennan DH,Emili A</div> <div itemprop="description">MA3-019 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse</div> <div itemprodp="datePublished">Sat Mar 01 00:00:00 EST 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17521381"> <div itemprop="name">Activated somatostatin type 2 receptors traffic in vivo in central neurons from dendrites to the trans Golgi before recycling.</div> </a> <div itemprop="about">Traffic (Copenhagen, Denmark)</div> <div itemprop="author">Csaba Z,Lelouvier B,Viollet C,El Ghouzzi V,Toyama K,Videau C,Bernard V,Dournaud P</div> <div itemprop="description">MA3-019 was used in immunohistochemistry to investigate the trafficking and recycling of activated somatostatin type 2 receptors in vivo in central neurons</div> <div itemprodp="datePublished">Sun Jul 01 00:00:00 EDT 2007</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15601667"> <div itemprop="name">The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum.</div> </a> <div itemprop="about">Journal of leukocyte biology</div> <div itemprop="author">Eleveld-Trancikova D,Triantis V,Moulin V,Looman MW,Wijers M,Fransen JA,Lemckert AA,Havenga MJ,Figdor CG,Janssen RA,Adema GJ</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to characterize the dendritic cell-derived protein DC-STAMP</div> <div itemprodp="datePublished">Tue Mar 01 00:00:00 EST 2005</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15601667"> <div itemprop="name">The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum.</div> </a> <div itemprop="about">Journal of leukocyte biology</div> <div itemprop="author">Eleveld-Trancikova D,Triantis V,Moulin V,Looman MW,Wijers M,Fransen JA,Lemckert AA,Havenga MJ,Figdor CG,Janssen RA,Adema GJ</div> <div itemprop="description">MA3-019 was used in immunocytochemistry to characterize the dendritic cell-derived protein DC-STAMP</div> <div itemprodp="datePublished">Tue Mar 01 00:00:00 EST 2005</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15684334"> <div itemprop="name">Restricted distribution of mRNAs encoding a sarcoplasmic reticulum or transverse tubule protein in skeletal myofibers.</div> </a> <div itemprop="about">The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society</div> <div itemprop="author">Nissinen M,Kaisto T,Salmela P,Peltonen J,Metsikkö K</div> <div itemprop="description">MA3-019 was used in immunohistochemistry to investigate the distribution of CSQ and DHPR proteins and corresponding mRNAs during myogenic development</div> <div itemprodp="datePublished">Tue Feb 01 00:00:00 EST 2005</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15020602"> <div itemprop="name">A cellular UDP-glucose deficiency causes overexpression of glucose/oxygen-regulated proteins independent of the endoplasmic reticulum stress elements.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Flores-Diaz M,Higuita JC,Florin I,Okada T,Pollesello P,Bergman T,Thelestam M,Mori K,Alape-Giron A</div> <div itemprop="description">MA3-019 was used in western blot to determine the effect of cellular UDP-Glc level on stress protein expression.</div> <div itemprodp="datePublished">Fri May 21 00:00:00 EDT 2004</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15105436"> <div itemprop="name">Intracellularly located misfolded glycoprotein hormone receptors associate with different chaperone proteins than their cognate wild-type receptors.</div> </a> <div itemprop="about">Molecular endocrinology (Baltimore, Md.)</div> <div itemprop="author">Mizrachi D,Segaloff DL</div> <div itemprop="description">MA3-019 was used in immunoprecipitation to study the folding of the glycoprotein hormone receptors and their misfolded mutants .</div> <div itemprodp="datePublished">Thu Jul 01 00:00:00 EDT 2004</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/12791669"> <div itemprop="name">Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin alpha2beta1.</div> </a> <div itemprop="about">Blood</div> <div itemprop="author">Lahav J,Wijnen EM,Hess O,Hamaia SW,Griffiths D,Makris M,Knight CG,Essex DW,Farndale RW</div> <div itemprop="description">MA3-019 was used in blocking/activating experiment to investigate the role of enzymatically catalyzed disulfide exchange for platelet adhesion to collagen via integrin alpha2beta1</div> <div itemprodp="datePublished">Mon Sep 15 00:00:00 EDT 2003</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/12065480"> <div itemprop="name">Protein 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</div> </noscript> </div> <div class="border-bottom"></div> <div class="product-bioinformatics-details"> <div class="container"> <h2 class="pdp-sub-heading">Bioinformatics</h2> <div class="row"> <div class="large-span-6 medium-span-12 small-span-12"> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="proteinContent"> <strong>Protein Aliases:</strong> CaO19.12595; Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; glutathione-insulin transhydrogenase; p55; p58; PDI; PDI1P; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase family A, member 1; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55 </p> <p class="view-buttons" id="ptnViewBtn" ng-class="$ctrl.bioContentLines('proteinContent', 'ptnViewBtn')"> <a id="proteinViewMoreBtn" ng-click="$ctrl.bioContentViewMore('proteinViewMoreBtn', 'proteinViewLessBtn', 'proteinContent')"> View more </a> <a id="proteinViewLessBtn" ng-click="$ctrl.bioContentViewLess('proteinViewLessBtn', 'proteinViewMoreBtn', 'proteinContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="geneContent"> <strong>Gene Aliases:</strong> CLCRP1; DSI; ERBA2L; ERp59; GIT; I79_002399; P4HB; P4Hbeta; p58; PDI; PDIA1; PDIR; PHDB; PO4DB; PO4HB; PROHB; Thbp </p> <p class="view-buttons" id="geneViewBtn" ng-class="$ctrl.bioContentLines('geneContent', 'geneViewBtn')"> <a id="geneViewMoreBtn" ng-click="$ctrl.bioContentViewMore('geneViewMoreBtn', 'geneViewLessBtn', 'geneContent')"> View more </a> <a id="geneViewLessBtn" ng-click="$ctrl.bioContentViewLess('geneViewLessBtn', 'geneViewMoreBtn', 'geneContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="unitProContent"> <strong>UniProt ID:</strong> <a href="http://www.uniprot.org/uniprot/P07237" target="_blank">(Human) P07237</a>, <a href="http://www.uniprot.org/uniprot/P04785" target="_blank">(Rat) P04785</a>, <a href="http://www.uniprot.org/uniprot/P09103" target="_blank">(Mouse) P09103</a>, <a href="http://www.uniprot.org/uniprot/Q8R4U2" target="_blank">(Chinese hamster) Q8R4U2</a> </p> <p class="view-buttons" id="unitViewBtn" ng-class="$ctrl.bioContentLines('unitProContent', 'unitViewBtn')"> <a id="unitProViewMoreBtn" ng-click="$ctrl.bioContentViewMore('unitProViewMoreBtn', 'unitProViewLessBtn', 'unitProContent')"> View more </a> <a id="unitProViewLessBtn" ng-click="$ctrl.bioContentViewLess('unitProViewLessBtn', 'unitProViewMoreBtn', 'unitProContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="entrezContent"> <strong>Entrez Gene ID:</strong> <a href="http://www.ncbi.nlm.nih.gov/gene?term=110255932" target="_blank">(Pig) 110255932</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=5034" target="_blank">(Human) 5034</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=25506" target="_blank">(Rat) 25506</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=18453" target="_blank">(Mouse) 18453</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=100689433" target="_blank">(Chinese hamster) 100689433</a> </p> <p class="view-buttons" id="etzViewBtn" ng-class="$ctrl.bioContentLines('entrezContent', 'etzViewBtn')"> <a id="entrezViewMoreBtn" ng-click="$ctrl.bioContentViewMore('entrezViewMoreBtn', 'entrezViewLessBtn', 'entrezContent')"> View more </a> <a id="entrezViewLessBtn" ng-click="$ctrl.bioContentViewLess('entrezViewLessBtn', 'entrezViewMoreBtn', 'entrezContent')"> View less </a> </p> </div> </div> <div class="large-span-6 medium-span-12 small-span-12"> <div class="accordion" id="accordion-bioinformatics"> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseOne"> Function(s) </a> </div> <div id="collapseOne" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/protein disulfide isomerase activity"> protein disulfide isomerase activity </a> <a class="icon" href="/antibody/primary/panther/procollagen-proline 4-dioxygenase activity"> procollagen-proline 4-dioxygenase activity </a> <a class="icon" href="/antibody/primary/panther/integrin binding"> integrin binding </a> <a class="icon" href="/antibody/primary/panther/protein binding"> protein binding </a> <a class="icon" href="/antibody/primary/panther/enzyme binding"> enzyme binding </a> <a class="icon" href="/antibody/primary/panther/poly(A) RNA binding"> poly(A) RNA binding </a> <a class="icon" href="/antibody/primary/panther/protein heterodimerization activity"> protein heterodimerization activity </a> <a class="icon" href="/antibody/primary/panther/peptidyl-proline 4-dioxygenase activity"> peptidyl-proline 4-dioxygenase activity </a> <a class="icon" href="/antibody/primary/panther/isomerase activity"> isomerase activity </a> <a class="icon" href="/antibody/primary/panther/chaperone"> chaperone </a> </div> </div> </div> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseTwo"> Process(es) </a> </div> <div id="collapseTwo" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/response to reactive oxygen species"> response to reactive oxygen species </a> <a class="icon" href="/antibody/primary/panther/protein folding"> protein folding </a> <a class="icon" href="/antibody/primary/panther/peptidyl-proline hydroxylation to 4-hydroxy-L-proline"> peptidyl-proline hydroxylation to 4-hydroxy-L-proline </a> <a class="icon" href="/antibody/primary/panther/response to endoplasmic reticulum stress"> response to endoplasmic reticulum stress </a> <a class="icon" href="/antibody/primary/panther/lipoprotein biosynthetic process"> lipoprotein biosynthetic process </a> <a class="icon" href="/antibody/primary/panther/cell redox homeostasis"> cell redox homeostasis </a> <a class="icon" href="/antibody/primary/panther/positive regulation of viral entry into host cell"> positive regulation of viral entry into host cell </a> <a class="icon" href="/antibody/primary/panther/cellular response to hypoxia"> cellular response to hypoxia </a> <a class="icon" href="/antibody/primary/panther/regulation of oxidative stress-induced intrinsic apoptotic signaling pathway"> regulation of oxidative stress-induced intrinsic apoptotic signaling pathway </a> </div> </div> </div> </div> </div> </div> </div> </div> <div class="recommended-products border-bottom hidden">  It has to be done as per old AB suggested Products section. </div> <script type="text/javascript" src="/order/genome-database/assets/core/js/magellanCore.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript"> $m.addPerformanceMark('abPDPScripts:loadBegin'); </script> <script type="text/javascript" src="/order/genome-database/assets/js/shared-module/dist/angular108-document-scripts.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript" src="/order/genome-database/assets/js/shared-module/dist/pdp-combined-scripts.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript" 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By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-019 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-019 has been shown to inhibit the activity of PDI in vitro. 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protein."},{"name":"Conjugate","value":"Unconjugated "},{"name":"Form","value":"Liquid"},{"name":"Concentration","value":"1 mg/mL"},{"name":"Purification","value":"Protein A"},{"name":"Storage buffer","value":"PBS with 1mg/mL BSA"},{"name":"Contains","value":"0.05% sodium azide"},{"name":"Storage conditions","value":"-20° C, Avoid Freeze/Thaw Cycles"},{"name":"Shipping conditions","value":"Wet ice"},{"name":"RRID","value":"AB_2163120"}]'; $m.userKey = "NONE"; angular.element(document).ready(function() { angular.bootstrap('#pdp-components', ['antibodyPdpModule']); }); THERMO_FISHER.pdp.sharedListComponent = THERMO_FISHER.pdp.sharedListComponent || {}; THERMO_FISHER.pdp.sharedListComponent.sharedList = null; </script> </div> <div class="documents"> <div class="documents-cntr" id="documents-container"> <div class="loading-wheel-lg hidden" id="documentLoadingWheel"></div> <div id="documentsUIRoot1"></div> </div>  <script defer type="text/javascript" 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