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Disponible en 5 langues." > <label id="p-lang-btn-label" for="p-lang-btn-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet cdx-button--action-progressive mw-portlet-lang-heading-5" aria-hidden="true" ><span class="vector-icon mw-ui-icon-language-progressive mw-ui-icon-wikimedia-language-progressive"></span> <span class="vector-dropdown-label-text">5 langues</span> </label> <div class="vector-dropdown-content"> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li class="interlanguage-link interwiki-de mw-list-item"><a href="https://de.wikipedia.org/wiki/Diethylaminoethylcellulose" title="Diethylaminoethylcellulose – allemand" lang="de" hreflang="de" data-title="Diethylaminoethylcellulose" data-language-autonym="Deutsch" data-language-local-name="allemand" class="interlanguage-link-target"><span>Deutsch</span></a></li><li class="interlanguage-link interwiki-en mw-list-item"><a href="https://en.wikipedia.org/wiki/Diethylaminoethyl_cellulose" title="Diethylaminoethyl cellulose – anglais" lang="en" hreflang="en" data-title="Diethylaminoethyl cellulose" data-language-autonym="English" data-language-local-name="anglais" class="interlanguage-link-target"><span>English</span></a></li><li class="interlanguage-link interwiki-ja mw-list-item"><a href="https://ja.wikipedia.org/wiki/%E3%82%B8%E3%82%A8%E3%83%81%E3%83%AB%E3%82%A2%E3%83%9F%E3%83%8E%E3%82%A8%E3%83%81%E3%83%AB%E3%82%BB%E3%83%AB%E3%83%AD%E3%83%BC%E3%82%B9" title="ジエチルアミノエチルセルロース – japonais" lang="ja" hreflang="ja" data-title="ジエチルアミノエチルセルロース" data-language-autonym="日本語" data-language-local-name="japonais" class="interlanguage-link-target"><span>日本語</span></a></li><li class="interlanguage-link interwiki-ko mw-list-item"><a href="https://ko.wikipedia.org/wiki/%EB%8B%A4%EC%9D%B4%EC%97%90%ED%8B%B8%EC%95%84%EB%AF%B8%EB%85%B8%EC%97%90%ED%8B%B8_%EC%85%80%EB%A3%B0%EB%A1%9C%EC%8A%A4" title="다이에틸아미노에틸 셀룰로스 – coréen" lang="ko" hreflang="ko" data-title="다이에틸아미노에틸 셀룰로스" data-language-autonym="한국어" data-language-local-name="coréen" class="interlanguage-link-target"><span>한국어</span></a></li><li class="interlanguage-link interwiki-pt mw-list-item"><a href="https://pt.wikipedia.org/wiki/Dietilaminoetil_celulose" title="Dietilaminoetil celulose – portugais" lang="pt" hreflang="pt" data-title="Dietilaminoetil celulose" data-language-autonym="Português" data-language-local-name="portugais" class="interlanguage-link-target"><span>Português</span></a></li> </ul> <div class="after-portlet after-portlet-lang"><span class="wb-langlinks-edit wb-langlinks-link"><a href="https://www.wikidata.org/wiki/Special:EntityPage/Q411341#sitelinks-wikipedia" title="Modifier les liens interlangues" class="wbc-editpage">Modifier les liens</a></span></div> </div> </div> </div> </header> <div class="vector-page-toolbar"> <div class="vector-page-toolbar-container"> <div id="left-navigation"> <nav aria-label="Espaces de noms"> <div 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typeof="mw:File/Thumb"><a href="/wiki/Fichier:Diethylaminoethylcellulose.png" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/9/98/Diethylaminoethylcellulose.png/220px-Diethylaminoethylcellulose.png" decoding="async" width="220" height="75" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/9/98/Diethylaminoethylcellulose.png/330px-Diethylaminoethylcellulose.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/9/98/Diethylaminoethylcellulose.png/440px-Diethylaminoethylcellulose.png 2x" data-file-width="692" data-file-height="236" /></a><figcaption>Structure de la cellulose DEAE</figcaption></figure> <p>La <b>cellulose diéthylaminoéthyle</b> (DEAE-C) est une résine chargée positivement utilisée en <a href="/wiki/Chromatographie_%C3%A0_%C3%A9change_d%27ions" title="Chromatographie à échange d'ions">chromatographie échangeuse d'anions</a> (voir <a href="/wiki/Chromatographie_en_phase_liquide" title="Chromatographie en phase liquide">Chromatographie en phase liquide</a>) permettant la rétention (à <a href="/wiki/Potentiel_hydrog%C3%A8ne" title="Potentiel hydrogène">pH</a> <a href="/wiki/Acide" title="Acide">basique</a>) et la séparation spécifique de <a href="/wiki/Mol%C3%A9cule" title="Molécule">molécules</a> chargées négativement. La force de rétention qui régit l'association entre les molécules chargées et la matrice dépend de la <a href="/wiki/Force_ionique" title="Force ionique">force ionique</a> et du pH de la solution d'<a href="/wiki/%C3%89lution" title="Élution">élution</a>. Elle peut être modulée en agissant sur ces deux composantes, ce qui conduit à la séparation. </p><p>La <a href="/wiki/Cellulose" title="Cellulose">cellulose</a> DEAE est très largement utilisée pour la <a href="/wiki/Purification_des_prot%C3%A9ines" title="Purification des protéines">purification des protéines</a> en solution. Les protéines retenues sur la colonne peuvent être libérées progressivement : </p> <ul><li>en augmentant la concentration saline d'un ion compétiteur de force comparable ;</li> <li>en utilisant un ion compétiteur de même concentration mais de force plus élevée ;</li> <li>en élevant le pH, ce qui modifie la charge de la protéine retenue.</li></ul> <p>La récupération des protéines éluées de la colonne est réalisée au moyen d'un collecteur de fractions. Un test d'activité spécifique de la protéine permet de sélectionner les fractions les plus riches en protéine désirée, ce qui permet la <a href="/wiki/Purification_(chimie)" title="Purification (chimie)">purification</a> de celle-ci. </p> <div class="mw-heading mw-heading2"><h2 id="Références"><span id="R.C3.A9f.C3.A9rences"></span>Références</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Cellulose_di%C3%A9thylamino%C3%A9thyle&veaction=edit&section=1" title="Modifier la section : Références" class="mw-editsection-visualeditor"><span>modifier</span></a><span class="mw-editsection-divider"> | </span><a href="/w/index.php?title=Cellulose_di%C3%A9thylamino%C3%A9thyle&action=edit&section=1" title="Modifier le code source de la section : Références"><span>modifier le code</span></a><span class="mw-editsection-bracket">]</span></span></div> <ul><li><abbr class="abbr indicateur-langue" title="Langue : anglais">(en)</abbr> Cet article est partiellement ou en totalité issu de l’article de Wikipédia en anglais intitulé <span class="plainlinks">« <a class="external text" href="https://en.wikipedia.org/wiki/Diethylaminoethyl_cellulose?oldid=159093483">Diethylaminoethyl cellulose</a> » <small>(<a class="external text" href="https://en.wikipedia.org/wiki/Diethylaminoethyl_cellulose?action=history">voir la liste des auteurs</a>)</small></span>.</li></ul> <div class="navbox-container" style="clear:both;"> <table class="navbox collapsible noprint autocollapse" style=""> <tbody><tr><th class="navbox-title" colspan="2" style=""><div style="float:left; width:6em; text-align:left"><div class="noprint plainlinks nowrap tnavbar" style="padding:0; font-size:xx-small; color:var(--color-emphasized, #000000);"><a href="/wiki/Mod%C3%A8le:Palette_S%C3%A9paration_et_purification_des_prot%C3%A9ines" title="Modèle:Palette Séparation et purification des protéines"><abbr class="abbr" title="Voir ce modèle.">v</abbr></a> · <a class="external text" href="https://fr.wikipedia.org/w/index.php?title=Mod%C3%A8le:Palette_S%C3%A9paration_et_purification_des_prot%C3%A9ines&action=edit"><abbr class="abbr" title="Modifier ce modèle. Merci de prévisualiser avant de sauvegarder.">m</abbr></a></div></div><div style="font-size:110%">Séparation et <a href="/wiki/Purification_des_prot%C3%A9ines" title="Purification des protéines">purification</a> des <a href="/wiki/Prot%C3%A9ine" title="Protéine">protéines</a></div></th> </tr> <tr> <th class="navbox-group" style="">par différence de <a href="/wiki/Solubilit%C3%A9" title="Solubilité">solubilité</a></th> <td class="navbox-list" style=""><div class="liste-horizontale"> <ul><li>Variation de la <a href="/wiki/Force_ionique" title="Force ionique">force ionique</a> <ul><li><a href="/wiki/Solubilisation_saline" title="Solubilisation saline">Solubilisation saline (salting in)</a></li> <li><a href="/wiki/Relargage" title="Relargage">Précipitation saline ou relargage (saling out)</a></li></ul></li> <li>Variation du <a href="/wiki/PH" class="mw-redirect" title="PH">pH</a></li> <li>Fractionnement par répartition entre phase polaire et phase apolaire</li></ul> </div></td> </tr> <tr> <th class="navbox-group" style="">par différence de <a href="/wiki/Charge_%C3%A9lectrique" title="Charge électrique">charge ionique</a></th> <td class="navbox-list navbox-even" style=""><table class="navbox-subgroup" style=""> <tbody><tr> <th class="navbox-group" style="width:90px"><a href="/wiki/%C3%89lectrophor%C3%A8se" title="Électrophorèse">Électrophorèse</a></th> <td class="navbox-list" style=";background:#ECECFF; text-align:left;"><div class="liste-horizontale"> <ul><li><a href="/wiki/%C3%89lectrophor%C3%A8se_capillaire" title="Électrophorèse capillaire">Électrophorèse capillaire</a></li> <li><a href="/wiki/%C3%89lectrophor%C3%A8se_sur_gel_d%27agarose" title="Électrophorèse sur gel d'agarose">Électrophorèse sur gel d'agarose</a></li> <li><a href="/wiki/%C3%89lectrophor%C3%A8se_sur_gel_de_polyacrylamide" title="Électrophorèse sur gel de polyacrylamide">Électrophorèse sur gel de polyacrylamide</a></li> <li><a href="/wiki/Focalisation_iso%C3%A9lectrique" title="Focalisation isoélectrique">Focalisation isoélectrique</a></li></ul> </div></td> </tr> <tr> <th class="navbox-group" style="width:90px"><a href="/wiki/Chromatographie" title="Chromatographie">Chromatographie</a></th> <td class="navbox-list navbox-even" style=";background:#ECECFF; text-align:left;"><div class="liste-horizontale"> <ul><li><a href="/wiki/Chromatographie_%C3%A0_%C3%A9change_d%27ions" title="Chromatographie à échange d'ions">Chromatographie à échange d'ions</a> <ul><li><a class="mw-selflink selflink">DEAE-C</a> : échange d'anions</li> <li><a href="/wiki/Carboxym%C3%A9thylcellulose" title="Carboxyméthylcellulose">CM-C</a> : échange de cations</li></ul></li></ul> </div></td> </tr> </tbody></table></td> </tr> <tr> <th class="navbox-group" style="">par différence de <a href="/wiki/Masse_mol%C3%A9culaire" title="Masse moléculaire">taille moléculaire</a></th> <td class="navbox-list" style=""><table class="navbox-subgroup" style=""> <tbody><tr> <th class="navbox-group" style="width:90px">Électrophorèse</th> <td class="navbox-list" style=";background:#ECECFF; text-align:left;"><div class="liste-horizontale"> <ul><li><a href="/wiki/%C3%89lectrophor%C3%A8se_sur_gel_de_polyacrylamide_en_pr%C3%A9sence_de_dod%C3%A9cylsulfate_de_sodium" title="Électrophorèse sur gel de polyacrylamide en présence de dodécylsulfate de sodium">Électrophorèse sur gel de polyacrylamide en présence de dodécylsulfate de sodium</a></li></ul> </div></td> </tr> <tr> <th class="navbox-group" style="width:90px">Chromatographie</th> <td class="navbox-list navbox-even" style=";background:#ECECFF; text-align:left;"><div class="liste-horizontale"> <ul><li>Chromatographie par filtration sur gel, une méthode de <a href="/wiki/Chromatographie_d%E2%80%99exclusion_st%C3%A9rique" class="mw-redirect" title="Chromatographie d’exclusion stérique">chromatographie d’exclusion stérique</a></li></ul> </div></td> </tr> <tr> <td class="navbox-list" style=";background:#ECECFF; text-align:left;" colspan="2"><div class="liste-horizontale"> <ul><li><a href="/wiki/Ultracentrifugation" title="Ultracentrifugation">Ultracentrifugation</a></li> <li><a href="/wiki/Ultrafiltration" title="Ultrafiltration">Ultrafiltration</a></li> <li><a href="/wiki/Dialyse" title="Dialyse">Dialyse</a></li></ul> </div></td> </tr> </tbody></table></td> </tr> <tr> <th class="navbox-group" style="">par différence de <a href="/wiki/Polarit%C3%A9_(chimie)" title="Polarité (chimie)">polarité</a></th> <td class="navbox-list navbox-even" style=""><table class="navbox-subgroup" style=""> <tbody><tr> <th class="navbox-group" style="width:90px">Chromatographie</th> <td class="navbox-list" style=";background:#ECECFF; text-align:left;"><div class="liste-horizontale"> <ul><li><a href="/wiki/Chromatographie_sur_papier" title="Chromatographie sur papier">Chromatographie sur papier</a></li> <li><a href="/wiki/Chromatographie_d%27adsorption" title="Chromatographie d'adsorption">Chromatographie d'adsorption</a></li> <li><a href="/wiki/Chromatographie_en_phase_inverse" title="Chromatographie en phase inverse">Chromatographie de partage à polarité de phases inversée</a></li> <li><a href="/wiki/Chromatographie_d%27interaction_hydrophobe" title="Chromatographie d'interaction hydrophobe">Chromatographie d'interaction hydrophobe</a></li></ul> </div></td> </tr> </tbody></table></td> </tr> <tr> <th class="navbox-group" style="">par différence d'<a href="/wiki/Affinit%C3%A9_(biochimie)" title="Affinité (biochimie)">affinité</a> pour un <a href="/wiki/Ligand_(chimie)" title="Ligand (chimie)">ligand</a></th> <td class="navbox-list" style=""><table class="navbox-subgroup" style=""> <tbody><tr> <th class="navbox-group" style="width:90px">Chromatographie</th> <td class="navbox-list" style=";background:#ECECFF; text-align:left;"><div class="liste-horizontale"> <ul><li><a href="/wiki/Chromatographie_d%27affinit%C3%A9" title="Chromatographie d'affinité">Chromatographie d'affinité</a></li></ul> </div></td> </tr> </tbody></table></td> </tr> <tr> <th class="navbox-group" style="">Revues</th> <td class="navbox-list navbox-even" style=""><div class="liste-horizontale"> <ul><li><a href="/w/index.php?title=Protein_Expression_and_Purification&action=edit&redlink=1" class="new" title="Protein Expression and Purification (page inexistante)">Protein Expression and Purification</a></li></ul> </div></td> </tr> </tbody></table> </div> <ul id="bandeau-portail" class="bandeau-portail"><li><span class="bandeau-portail-element"><span class="bandeau-portail-icone"><span class="noviewer" typeof="mw:File"><a href="/wiki/Portail:Chimie" title="Portail de la chimie"><img alt="icône décorative" src="//upload.wikimedia.org/wikipedia/commons/thumb/5/59/Nuvola_apps_edu_science.svg/24px-Nuvola_apps_edu_science.svg.png" decoding="async" width="24" height="24" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/5/59/Nuvola_apps_edu_science.svg/36px-Nuvola_apps_edu_science.svg.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/5/59/Nuvola_apps_edu_science.svg/48px-Nuvola_apps_edu_science.svg.png 2x" data-file-width="128" data-file-height="128" /></a></span></span> <span class="bandeau-portail-texte"><a href="/wiki/Portail:Chimie" title="Portail:Chimie">Portail de la chimie</a></span> </span></li> <li><span class="bandeau-portail-element"><span class="bandeau-portail-icone"><span class="noviewer" typeof="mw:File"><a href="/wiki/Portail:Biochimie" title="Portail de la biochimie"><img alt="icône décorative" src="//upload.wikimedia.org/wikipedia/commons/thumb/1/19/Hemoglobin.jpg/26px-Hemoglobin.jpg" decoding="async" width="26" height="24" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/1/19/Hemoglobin.jpg/39px-Hemoglobin.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/1/19/Hemoglobin.jpg/52px-Hemoglobin.jpg 2x" data-file-width="418" data-file-height="386" /></a></span></span> <span class="bandeau-portail-texte"><a href="/wiki/Portail:Biochimie" title="Portail:Biochimie">Portail de la biochimie</a></span> </span></li> </ul>    </div><noscript><img src="https://login.wikimedia.org/wiki/Special:CentralAutoLogin/start?type=1x1" alt="" width="1" height="1" style="border: none; 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