Viral Vector Characterization in Cell and Gene Therapy

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<div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_h1"> <div><h1>Viral Vector Characterization in Cell and Gene Therapy</h1></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><strong>Assessing critical quality attributes of viral vectors for cell and gene therapy applications.</strong></p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/banners/aav-lvv-vector-characterization-hero.png" alt="Adeno-associated viral and lentiviral vectors" fetchpriority="high" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/research-areas">Research Areas</a> </li> <li> <a href="/research-areas/cell-and-gene-therapy">Cell & Gene Therapy Research and Manufacturing Solutions</a> </li> <li> Viral Vector Characterization In Cell and Gene Therapy </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>Viral Vectors in Cell and Gene Therapy</h2> <p>Viral vectors are crucial to the development and manufacturing of many cell and gene therapies by facilitating successful gene delivery. Comprehensive characterization of these viral vectors through measurement of specific critical quality attributes (CQAs), such as purity and stability, is vital for ensuring the safety and efficacy of your therapy.</p> <p>By moving to automated solutions for viral vector analysis, you can gain rapid insights and streamline QC processes for cell and gene therapy development and manufacturing.</p> <p>Discover multi-attribute platforms for adeno-associated virus (AAV) and lentiviral vector analysis below, or if you are seeking alternative gene delivery methods, learn more about our non-viral transposon system, <a href="/services/gene-engineering-services-tcbuster">TcBuster™</a>.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4 p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <a href="https://www.bio-techne.com/research-areas/cell-and-gene-therapy/viral-vector-characterization/aav" title="Explore Tools for AAV Characterization" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/aav-vector-characterization-525x300.png" alt="Adeno-associated viral vectors" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div><h2>AAV Vector Characterization</h2></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Explore streamlined characterization through automated, analytical multi-attribute solutions to expedite QC processes and ensure precise quantification and characterization of adeno-associated viral vectors.</p></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/research-areas/cell-and-gene-therapy/viral-vector-characterization/aav" class="btn btn-primary" type="link"> Explore Tools for AAV Characterization </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Lentiviral Vector Characterization</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Lentivirus is widely used to transduce HEK cells for engineered immune cell therapies, such as CAR-T and CAR-NK, and as a viral vector for gene therapy. With increasing application in <em>in vivo</em> and <em>ex vivo</em> gene delivery for various genetic diseases, the qualification of clinical-grade lentiviral vectors (LVVs) must adhere to stringent manufacturing and quality standards. Make the move to automated analytical solutions to streamline QC testing and provide thorough lentivirus characterization faster.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lvv-vector-characterization-525x300.png" alt="Lentiviral Vector" fetchpriority="high" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/hiv-gag-p24-assay-graph-525x300.png" alt="HIV Gag p24 Assay for measuring lentiviral titer" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>Lentiviral Titer Determination</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Accurately quantify lentivirus titer in less than 90 minutes with the Simple Plex™ HIV p24 Assay. This automated assay minimizes user error, making it an ideal tool for quality-focused, standardized, and scalable processes in GMP environments.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/resources/instrument-applications/simple-plex-viral-titer-assays/p24-assay" class="btn btn-primary" type="link"> Analyze LVV Titer </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/hcp-assay-graph-525x300.png" alt="Simple Plex HEK 293 host cell protein (HCP) Assay vs. Cygnus HEK 293 HCP ELISA" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>Residual Host Cell Protein Quantitation</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Assess purity with the Simple Plex HEK 293 Host Cell Protein (HCP) 3G Assay, co-developed with industry leader Cygnus. Combining the accuracy of a Cygnus ELISA with a streamlined workflow, this assay ensures precise detection at each purification stage. </p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/resources/instrument-applications/simple-plex-cell-therapy#HHCP" class="btn btn-primary" type="link"> Quantitate HEK 293 HCPs </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/lvv-identity-graph-525x300.png" alt="LVV Identity Data Figure" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>Comprehensive LVV Characterization</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Automate LVV characterization with a multi-attribute platform for analyzing CQAs, such as identity and titer. Maurice™ combines CE-SDS and ciEF to overcome the limitations of manual techniques and deliver comprehensive LVV analysis in record time.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/resources/instrument-applications/ce-sds-with-maurice#applications" class="btn btn-primary" type="link"> Analyze LVV CQAs </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/v4_LVV-capsid-content-analysis-data.png" alt="Lentiviral vector capsid content analysis using Simple Western" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>Quantify LVV CQAs in Complex Samples</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Characterize your LVVs further upstream with Simple Western™. Simultaneously measure multiple LVV CQAs, including viral titer, stability, purity, and capsid content, in cell lysates and tissue homogenates. Get the information you need early on for smarter process development.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/resources/literature/lentiviral-vector-analysis-cell-and-gene-therapy-made-simple" class="btn btn-primary" type="link"> Inform Downstream Decisions </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Resources on Viral Vector Characterization</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/webinars/-gene-therapy-symposium" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/webinars/challenges-gt-analytics-webinar-mockup.png" alt="Lentiviral vector identity analysis using Maurice" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/webinars/-gene-therapy-symposium" > <h3 class="card_title_htag"> <div>Overcoming Challenges in Gene Therapy Analytics</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/webinars/-gene-therapy-symposium" > Watch Symposium On-Demand </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/aav-analysis-for-gene-therapy-with-maurice-ce-sds" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/an-aav-ce-sds-plus-vs-turbo-thumb-650x500.png" alt="Viral Vector Characterization Application Note" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/aav-analysis-for-gene-therapy-with-maurice-ce-sds" > <h3 class="card_title_htag"> <div>Characterize Your Viral Vectors from Discovery to GMP Release</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/aav-analysis-for-gene-therapy-with-maurice-ce-sds" > Read Application Note </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/webinars/simple-western-optimizes-AAV-manufacturing" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div 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Viral Vector Characterization in Cell and Gene Therapy | Bio-Techne