Cultrex In Vitro Angiogenesis Assay (Tube Formation) (3470-096-K) by R&D Systems, Part of Bio-Techne

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Backed by our 100% Guarantee." /> <script type="application/ld+json" id="json-ld">[{"@context":"http://schema.org","@type":"Product","additionalProperty":[],"aggregateRating":{"@type":"AggregateRating","ratingValue":5,"reviewCount":4,"worstRating":"1","bestRating":"5"},"alternateName":"","brand":{"@type":"Brand","name":"R&D Systems"},"description":"Cultrex In Vitro Angiogenesis Assay (Tube Formation)","image":[],"isRelatedTo":[],"itemcondition":"new","mpn":"3470-096-K","name":"Cultrex In Vitro Angiogenesis Assay (Tube Formation)","productID":"3470-096-K","review":[{"@type":"Review","author":{"@type":"Person","name":"Anonymous"},"datePublished":"Mar 1 2022 11:15AM","name":"Cultrex In Vitro Angiogenesis Assay (Tube Formation)","reviewRating":{"@type":"Rating","bestRating":"5","ratingValue":5,"worstRating":"1"}},{"@type":"Review","author":{"@type":"Person","name":"Anonymous"},"datePublished":"May 9 2016 7:30PM","name":"Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well","reviewRating":{"@type":"Rating","bestRating":"5","ratingValue":5,"worstRating":"1"}},{"@type":"Review","author":{"@type":"Person","name":"Anonymous"},"datePublished":"May 9 2016 7:30PM","name":"Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well","reviewRating":{"@type":"Rating","bestRating":"5","ratingValue":5,"worstRating":"1"}},{"@type":"Review","author":{"@type":"Person","name":"Anonymous"},"datePublished":"May 9 2016 7:03PM","name":"Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well","reviewRating":{"@type":"Rating","bestRating":"5","ratingValue":4,"worstRating":"1"}}],"sku":"3470-096-K","url":"https://www.bio-techne.com/p/cell-culture/cultrex-in-vitro-angiogenesis-assay-tube-formation_3470-096-k"},{"@context":"http://schema.org","@type":"AskAction","question":[{"@context":"http://schema.org","@type":"Question","name":"What is the Tube Formation Assay?","identifier":{"@type":"PropertyValue","propertyID":"FaqId","value":34051},"acceptedAnswer":{"@type":"Answer","text":"The Tube Formation Assay is based on the ability of endothelial cells to form three-dimensional capillary-like tubular structures when cultured on a hydrogel of reconstituted basement membrane, such as Cultrex Basement Membrane Extract (BME)."}},{"@context":"http://schema.org","@type":"Question","name":"What are the advantages of the Tube Formation Assay?","identifier":{"@type":"PropertyValue","propertyID":"FaqId","value":34052},"acceptedAnswer":{"@type":"Answer","text":"The Tube Formation Assay is the most widely used in vitro angiogenesis assay. The assay is rapid, inexpensive and quantifiable. It can be used to identify potentially angiogenic and anti-angiogenic factors, to determine endothelial cell phenotype, and to study pathways and mechanisms involved in angiogenesis. It can be performed in a high throughput mode to screen for a large number of compounds."}},{"@context":"http://schema.org","@type":"Question","name":"What cell types can be used in the Tube Formation Assay?","identifier":{"@type":"PropertyValue","propertyID":"FaqId","value":34053},"acceptedAnswer":{"@type":"Answer","text":"The Tube Formation Assay is specific for endothelial cells, either primary cells or immortalized cell lines. Only endothelial cells form capillary-like structures with a lumen inside. Other endothelial cell types form other structures."}},{"@context":"http://schema.org","@type":"Question","name":"What are the variables associated with the Tube Formation Assay?","identifier":{"@type":"PropertyValue","propertyID":"FaqId","value":34054},"acceptedAnswer":{"@type":"Answer","text":"The major variables associated with tube formation are composition of the Cultrex Basement Membrane Extract (BME) hydrogel, thickness of the hydrogel, cell density, composition of angiogenic factors in the assay medium, and assay period."}},{"@context":"http://schema.org","@type":"Question","name":"Which Cultrex Basement Membrane Extract (BME) should I use for the Tube Formation Assay?","identifier":{"@type":"PropertyValue","propertyID":"FaqId","value":34055},"acceptedAnswer":{"@type":"Answer","text":"Cultrex Reduced Growth Factor BME (RGF BME) is generally used for testing compounds that promote angiogenesis because formation of capillary-like structures (tubes) is significantly less compared to non-growth factor reduced varieties of Cultrex BME. The Cultrex In Vitro Angiogeneis Assay (Tube Formation) includes a qualified production lot of Cultrex RGF BME that exhibits reduced background tube formation in the absence of angiogenic factors."}},{"@context":"http://schema.org","@type":"Question","name":"How do I reduce spontaneous formation of tubular structures on Cultrex BME in the absence of angiogenic factors?","identifier":{"@type":"PropertyValue","propertyID":"FaqId","value":34056},"acceptedAnswer":{"@type":"Answer","text":"Primary endothelial cells, such as Human Umbilical Vein Endothelial Cells (HUVECs) form capillary-like structures in the absence of added angiogenic factors less often than immortalized endothelial cells. Generally, reducing the number of cells per cm2 plated onto Cultrex BME will result in less background or spontaneous tube formation. Titrate the number of cells and find optimal conditions for your specific cell line. When endothelial cells fully form capillary structures in response to angiogenic activators, but not in their absence, you may proceed."}}]}]</script> <link rel="alternate" hreflang="en" href="https://www.bio-techne.com/p/cell-culture/cultrex-in-vitro-angiogenesis-assay-tube-formation_3470-096-k" /> <link rel="alternate" hreflang="zh-hans" href="https://www.bio-techne.com/cn/p/cell-culture/cultrex-in-vitro-angiogenesis-assay-tube-formation_3470-096-k" /> <link rel="icon" href="/themes/custom/bio_techne_global/favicon.ico" type="image/vnd.microsoft.icon" /> <link rel="preconnect" href="https://resources.bio-techne.com" /> <link rel="preconnect" href="https://resources.tocris.com" /> <link rel="preconnect" href="https://i.icomoon.io" /> <link rel="preconnect" href="https://cdn.icomoon.io" /> <link rel="dns-prefetch" href="https://connect.facebook.net" /> <link rel="dns-prefetch" href="https://resources.bio-techne.com" /> <link rel="dns-prefetch" href="https://snap.licdn.com" /> <link 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class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13391">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" 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Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" 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href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup 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id="key_details_table_wrapper"><tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Features: </td> <td class="product-data-value py-2 align-top w-75 text-break"> Allows for the detection of inducers and inhibitors of endothelial cell tube formation. </td> </tr> </table> </div> </div> <div class="row border-top mb-2" id="images_atc_orderinfo_wrapper"><div class="col-xl-9 mt-2" id="image_atc_keydetails_wrapper"><div class="row" id="image_atc_wrapper"><div class="col-xl-4 order-xl-first" id="image_wrapper"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="//resources.bio-techne.com/images/1bt/bio-techne-logo.png" alt="Cultrex In Vitro Angiogenesis Assay (Tube Formation)" title="Cultrex In Vitro Angiogenesis Assay (Tube Formation)" data-caption="Cultrex In 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class="nav-link active" id="technical-data-tab-tab" href="#technical-data-tab" data-toggle="tab" role="tab" aria-controls="region-4821584720927" aria-selected="true" >Technical Data</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-reviews-faqs-tab-tab" href="#citations-reviews-faqs-tab" data-toggle="tab" role="tab" aria-controls="region-7631584720992" aria-selected="false" >Citations / Reviews (4) / FAQs</a> </li> </ul> <div class="tab-pane fade mx-4 py-4 show" id="procedure-tab" data-tab-label="Procedure" role="tabpanel" aria-labelledby="procedure-tab-tab"></div> <div class="tab-content rounded-bottom border-left border-right border-bottom mb-3" id="ProductTabContent"><div class="tab-pane fade show active mx-4 cell-culture-gpp" id="technical-data-tab" data-tab-label="Technical Data" role="tabpanel" aria-labelledby="technical-data-tab-tab"><div class="product_details_anchors"><div class="product-pages-anchors nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"><li id="product-summary_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor active" href="#product-summary" id="product-summary_li" >Product Summary</a> </li> <li id="kit-contents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#kit-contents" id="kit-contents_li" >Kit Contents</a> </li> <li id="product-specifications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#product-specifications" id="product-specifications_li" >Product Specifications</a> </li> <li id="preparation--storage_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#preparation--storage" id="preparation--storage_li" >Preparation & Storage</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents datasheet_static_link" href="#product-documents" id="product-documents_li" >Product Datasheets</a> </li> </div> <div id="product-summary" class="nav-link px-0" data-anchor-label="Product Summary"><div id="product_title_summary"><h3>Summary for Cultrex In Vitro Angiogenesis Assay (Tube Formation)</h3><h4> Why Use Cultrex In Vitro Angiogenesis Assay (Tube Formation), 96-well? </h4> <article class="product-data-wrapper"> <p>The Cultrex <em></em>in vitro</em></em> Angiogenesis Assay Tube Formation Kit was developed for the evaluation of inducers and inhibitors of endothelial cell tube formation. During the course of the assay, endothelial cells are seeded on a thick gel of Basement Membrane Extract in the presence of angiogenesis modulating agents. If the medium is permissive to the angiogenic response, cells will self assemble into tube networks within hours; if there are inhibitors in the medium, tube network formation will be incomplete or nonexistent. Sulforaphane [1- isothiocyanato-(4R)- methylsulfinyl)-butane], found in broccoli and other cruciferous vegetables, is a naturally occurring cancer chemopreventive agent, and is provided as a control inhibitor to visualize this process. Endothelial cells may be fluorescently labeled using Calcein AM, and images captured using a microscope camera may be analyze to quantify the angiogenic response. Cultrex <em></em>in vitro</em></em> Angiogenesis Assay Kit provides a cost-effective method for investigation of prospective angiogenesis inhibitors in a 96 well plate format.</p> </article> </div> </div> <div id="kit-contents" class="nav-link px-0" data-anchor-label="Kit Contents"><div id="kit_contents_wrapper"><h3>Kit Contents for Cultrex In Vitro Angiogenesis Assay (Tube Formation)</h3> <div id="kit_content"> <html><body><ul class="mt-4"><li>Cell Staining Solution</li><li>Cultrex BME, reduced growth factor without phenol red</li><li>Sulforaphane</li><li>Calcein AM<br><br>Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.</li></ul></body></html> </div> </div> </div> <div id="product-specifications" class="nav-link px-0" data-anchor-label="Product Specifications"><table class="tablesaw tablesaw-stack table-bordered table" id="product_specification_wrapper"><h3>Product Specifications for Cultrex In Vitro Angiogenesis Assay (Tube Formation)</h3><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Species</td> <td class="product-data-value p-3 px-4 text-break"> Multi-species </td> </tr> </table> </div> <div id="preparation--storage" class="nav-link px-0" data-anchor-label="Preparation & Storage"><div id="cc_prep_storage"><h3>Preparation & Storage</h3><table class="table-bordered table" id="prep_storage_wrapper"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Shipping Conditions</td> <td class="product-data-value p-3 px-4 text-break"> The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Storage</td> <td class="product-data-value p-3 px-4 text-break"> Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. </td> </tr> </table> </div> </div> <div id="product-datasheets-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Datasheets"></div> </div> </div> <div class="tab-pane fade mx-4 py-4 show" id="citations-reviews-faqs-tab" data-tab-label="Citations / Reviews (4) / FAQs" role="tabpanel" aria-labelledby="citations-reviews-faqs-tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#reviews"> Reviews </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#faqs"> FAQs </a> </li> </div> </div> <div id="citations"> <section id="citations-tab-content-placeholder" class="tab-content-placeholder" data-id="citations" data-product_code="3470-096-K"> <div class="spinner-border 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Cultrex In Vitro Angiogenesis Assay (Tube Formation) (3470-096-K) by R&D Systems, Part of Bio-Techne