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<!doctype html> <html lang="ja"> <head> <title>Understanding Sample Preparation Workflow: Semi-Automated Pr | 1099778</title> <meta name="keywords" content="Ramisa Fariha, Collin Hill, Mohannad Jabrah, Adam Spooner, Anubhav Tripathi*, "/> <meta name="description" content="High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the pro..1099778"/> <meta name="citation_publisher" content="ロングダム出版SL" /> <meta name="citation_journal_title" content="アンドロロジー - オープンアクセス"> <meta name="citation_title" content="Understanding Sample Preparation Workflow: Semi-Automated Preparation of Serum Testosterone for Detection Using LC-MS/MS"> <meta name="citation_author" content="Ramisa Fariha" /> <meta name="citation_author" content="Collin Hill" /> <meta name="citation_author" content="Mohannad Jabrah" /> <meta name="citation_author" content="Adam Spooner" /> <meta name="citation_author" content="Anubhav Tripathi" /> <meta name="citation_year" content=""> <meta name="citation_volume" content="13"> <meta name="citation_issue" content="5"> <meta name="citation_abstract" content="High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the progression of different metabolic disorders. Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS® G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), within a clinically relevant range of testosterone serum concentrations (5-1500 ng/dl). Moreover, the assay showcased LOD of 5 ng/dl, which is significantly comparable to commercially established diagnostic tools. The developed method also exhibited high linearity over the specified testosterone serum range, with an obtained linearity of R2>0.99 using both manual as well as automated sample preparation. Overall, the work shown presents an optimized, automatable LC-MS/MSbased testosterone diagnostic assay for the routine evaluation of patient testosterone levels in serum for clinical and research use."> <meta name="citation_abstract_html_url" content="https://japanese.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html"> <meta name="format-detection" content="telephone=no"> <meta http-equiv="Content-Language" content="ja"> <meta name="google-site-verification" content="NomPTP94YozsgvD3NEFpNqUfY88e0TU0L64zNzZTpd0" /> <meta itemprop="name" content="longdom" /> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="ROBOTS" content="INDEX,FOLLOW"> <meta name="googlebot" content="INDEX,FOLLOW"> <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no"> <link rel="canonical" 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Preparation of Serum Testosterone for Detection Using LC-MS/MS</h3> <p> <i class="fas fa-user"></i> Ramisa Fariha, Collin Hill, Mohannad Jabrah, Adam Spooner, Anubhav Tripathi* </p> <p>High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the progression of different metabolic disorders. Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS<sup>® </sup>G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), within a clinically relevant range of testosterone serum concentrations (5-1500 ng/dl). Moreover, the assay showcased LOD of 5 ng/dl, which is significantly comparable to commercially established diagnostic tools. The developed method also exhibited high linearity over the specified testosterone serum range, with an obtained linearity of R2>0.99 using both manual as well as automated sample preparation. Overall, the work shown presents an optimized, automatable LC-MS/MSbased testosterone diagnostic assay for the routine evaluation of patient testosterone levels in serum for clinical and research use.</p> <div class="alert alert-info text-left"><b>免責事項:</b> この要約は人工知能ツールを使用して翻訳されたものであり、まだレビューまたは検証されていません。</div> <div class="nav social-icons"> <a class="nav-link w-auto">この記事をシェアする</a> <a title="ここをクリック" target="_blank" href="https://www.facebook.com/sharer.php?u=https://japanese.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-facebook-f"></i></a> <a class="nav-link" title="ここをクリック" target="_blank" href="https://twitter.com/share?url=https://japanese.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-twitter"></i></a> <a class="nav-link" title="ここをクリック" target="_blank" href="https://www.linkedin.com/shareArticle?mini=true&url=https://japanese.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link" title="ここをクリック" target="_blank" href="https://plus.google.com/share?url=https://japanese.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-google-plus-g"></i></a> </div> </div> </div> </div> </section> <footer class="bg-blue-grey-900 py-3"> <div class="container"> <div class="row"> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">コンテンツリンク</h4> <ul class="list-unstyled footer-links font-size-3"> <li><a class="" href="https://japanese.longdom.org/privacy-policy.html" title="ここをクリック">プライバシーポリシー</a></li> <li><a class="" href="https://japanese.longdom.org/terms-conditions.html" title="ここをクリック">利用規約</a></li> <li><a class="" href="https://japanese.longdom.org/authors-reviewers-editors.html" title="ここをクリック">著者、査読者、編集者</a></li> </ul> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">ロングダムに連絡する</h4> <p><strong>ロングダムグループ SA</strong><br /> ロジェ・ヴァンデンドリッシュ通り、<br /> 18、1150 ブリュッセル、ベルギー<br /> 電話番号: +442038085340<br /> <strong>メール:</strong> <a class="white" href="mailto:info@longdom.org" title="ここをクリック">info@longdom.org</a></p> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">接続</h4> <nav class="nav nav-pills social-icons-footer flex-column a-pl-0"> <a href="https://www.facebook.com/longdompublisher" title="ここをクリック" target="_blank" class="nav-link bg-facebook-hover"><i class="fab fa-facebook-f bg-facebook"></i></a> <a href="https://www.linkedin.com/company/longdom-publishing-sl/" title="ここをクリック" target="_blank" class="nav-link bg-linkedin-hover"><i class="fab fa-linkedin-in bg-linkedin"></i></a> <a href="https://twitter.com/LongdomP" title="ここをクリック" target="_blank" class="nav-link bg-twitter-hover"><i class="fab fa-twitter bg-twitter"></i></a> <a href="https://www.instagram.com/longdom_publisher/" title="ここをクリック" target="_blank" class="nav-link bg-instagram-hover"><i class="fab fa-instagram bg-instagram"></i></a> </nav> </div> </div> <div class="row text-center"> <div class="col"> <p>著作権 © 2024 <a href="https://japanese.longdom.org/" title="ここをクリック" class="white">ロングダム出版SL</a>.</p> </div> </div> </div> </footer>  <a href="#0" class="cd-top js-cd-top">Top</a>   <script src="https://code.jquery.com/jquery-3.3.1.min.js"></script> <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js"></script> <script src="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js"></script>  <script> jQuery(function($) { $(window).scroll(function fix_element() { $('#target').css( $(window).scrollTop() > 100 ? 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