CINXE.COM

<!DOCTYPE html> <html lang="en" class="no-js"> <head> <meta charset="UTF-8"> <meta name="viewport" content="width=device-width, initial-scale=1.0, user-scalable=yes"> <title>Search</title> <meta id="meta-title" property="citation_title" content="Search"/> <meta id="og-title" property="og:title" content="Search"/> <meta name="twitter:widgets:autoload" content="off"/> <meta name="twitter:dnt" content="on"/> <meta name="twitter:widgets:csp" content="on"/> <meta name="google-site-verification" content="lQbRRf0vgPqMbnbCsgELjAjIIyJjiIWo917M7hBshvI"/> <meta id="og-image" property="og:image" content="https://escholarship.org/images/escholarship-facebook2.jpg"/> <meta id="og-image-width" property="og:image:width" content="1242"/> <meta id="og-image-height" property="og:image:height" content="1242"/> <link rel="stylesheet" href="/css/main-6e346ed4504727cd.css"> <link rel="resource" type="application/l10n" href="/node_modules/pdfjs-embed2/dist/locale/locale.properties"> <noscript><style> .jsonly { display: none } </style></noscript>   <script type="text/plain" data-type="application/javascript" data-name="matomo"> var _paq = window._paq = window._paq || []; /* tracker methods like "setCustomDimension" should be called before "trackPageView" */ _paq.push(['trackPageView']); _paq.push(['enableLinkTracking']); (function() { var u="//matomo.cdlib.org/"; _paq.push(['setTrackerUrl', u+'matomo.php']); _paq.push(['setSiteId', '7']); var d=document, g=d.createElement('script'), s=d.getElementsByTagName('script')[0]; g.async=true; g.src=u+'matomo.js'; s.parentNode.insertBefore(g,s); console.log('*** MATOMO LOADED ***'); })(); </script>  </head> <body> <div id="main"><div data-reactroot=""><div class="body"><a href="#maincontent" class="c-skipnav">Skip to main content</a><div class="l_search"><div><div style="margin-top:-10px"></div><header id="#top" class="c-header"><a class="c-header__logo2" href="/"><picture><source srcSet="/images/logo_eschol-small.svg" media="(min-width: 870px)"/><img src="/images/logo_eschol-mobile.svg" alt="eScholarship"/></picture><div class="c-header__logo2-tagline">Open Access Publications from the University of California</div></a><div class="c-header__search"><form class="c-search1"><label class="c-search1__label" for="c-search1__field">search</label><input type="search" id="c-search1__field" name="q" class="c-search1__field" placeholder="Search over 500,000 items" autoCapitalize="off" value="author:Hansen, Henrik"/><button type="submit" class="c-search1__submit-button" aria-label="submit search"></button><button type="button" class="c-search1__search-close-button" aria-label="close search field"></button></form></div><button class="c-header__search-open-button" aria-label="open search field"></button></header></div><div class="c-navbar"><nav class="c-nav"><details open="" class="c-nav__main"><summary class="c-nav__main-button">Menu</summary><ul class="c-nav__main-items"><li><details class="c-nav__sub"><summary class="c-nav__sub-button">About eScholarship</summary><div class="c-nav__sub-items"><button class="c-nav__sub-items-button" aria-label="return to menu">Main Menu</button><ul><li><a href="/aboutEschol">About eScholarship</a></li><li><a href="/repository">eScholarship Repository</a></li><li><a href="/publishing">eScholarship Publishing</a></li><li><a href="/policies">Site Policies</a></li><li><a href="/terms">Terms of Use and Copyright Information</a></li><li><a href="/privacyPolicy">Privacy Statement</a></li></ul></div></details></li><li><details class="c-nav__sub"><summary class="c-nav__sub-button">Campus Sites</summary><div class="c-nav__sub-items"><button class="c-nav__sub-items-button" aria-label="return to menu">Main Menu</button><ul><li><a href="/uc/ucb">UC Berkeley</a></li><li><a href="/uc/ucd">UC Davis</a></li><li><a href="/uc/uci">UC Irvine</a></li><li><a href="/uc/ucla">UCLA</a></li><li><a href="/uc/ucm">UC Merced</a></li><li><a href="/uc/ucr">UC Riverside</a></li><li><a href="/uc/ucsd">UC San Diego</a></li><li><a href="/uc/ucsf">UCSF</a></li><li><a href="/uc/ucsb">UC Santa Barbara</a></li><li><a href="/uc/ucsc">UC Santa Cruz</a></li><li><a href="/uc/ucop">UC Office of the President</a></li><li><a href="/uc/lbnl">Lawrence Berkeley National Laboratory</a></li><li><a href="/uc/anrcs">UC Agriculture & Natural Resources</a></li></ul></div></details></li><li><a href="/ucoapolicies">UC Open Access Policies</a></li><li><a href="/publishing">eScholarship Publishing</a></li><li><a href="/ucpubs">UCPUBS</a></li></ul></details></nav></div><form id="facetForm" class="c-columns"><aside><div><div class="c-filter"><h1 class="c-filter__heading">Your search: "author:Hansen, Henrik"</h1><input type="hidden" name="q" value="author:Hansen, Henrik"/><div class="c-filter__results">3 results</div><div class="c-filter__inactive-note">No filters applied</div><details class="c-filter__active" open=""><summary><span><strong></strong> filters applied</span></summary><button class="c-filter__clear-all">clear all</button><ul class="c-filter__active-list"></ul></details><a href="https://help.escholarship.org/support/solutions/articles/9000148939-using-advanced-search-beta-" class="c-filter__tips">Search tips</a></div><div class="c-refine--has-drawer"><button class="c-refine__button--open">Refine Results</button><button class="c-refine__button--close" hidden="">Back to Results</button><div class="c-refine__drawer--closed"><details class="c-facetbox" open=""><summary class="c-facetbox__summary"><span id="facetbox0">Type of Work</span></summary><fieldset aria-labelledby="facetbox0"><ul class="c-checkbox"><li class=""><input type="checkbox" id="type_of_work-article" class="c-checkbox__input" name="type_of_work" value="article"/><label for="type_of_work-article" class="c-checkbox__label">Article (3)</label></li><li class=""><input type="checkbox" id="type_of_work-monograph" class="c-checkbox__input" name="type_of_work" value="monograph"/><label for="type_of_work-monograph" class="c-checkbox__label">Book (0)</label></li><li class=""><input type="checkbox" id="type_of_work-dissertation" class="c-checkbox__input" name="type_of_work" value="dissertation"/><label for="type_of_work-dissertation" class="c-checkbox__label">Theses (0)</label></li><li class=""><input type="checkbox" id="type_of_work-multimedia" class="c-checkbox__input" name="type_of_work" value="multimedia"/><label for="type_of_work-multimedia" class="c-checkbox__label">Multimedia (0)</label></li></ul></fieldset></details><details class="c-facetbox" open=""><summary class="c-facetbox__summary"><span id="facetbox1">Peer Review</span></summary><fieldset aria-labelledby="facetbox1"><ul class="c-checkbox"><li class=""><input type="checkbox" id="peer_reviewed-1" class="c-checkbox__input" name="peer_reviewed" value="1"/><label for="peer_reviewed-1" class="c-checkbox__label">Peer-reviewed only (3)</label></li></ul></fieldset></details><details class="c-facetbox"><summary class="c-facetbox__summary"><span id="facetbox2">Supplemental Material</span></summary><fieldset aria-labelledby="facetbox2"><ul class="c-checkbox--2column"><li class=""><input type="checkbox" id="supp_file_types-video" class="c-checkbox__input" name="supp_file_types" value="video"/><label for="supp_file_types-video" class="c-checkbox__label">Video (0)</label></li><li class=""><input type="checkbox" id="supp_file_types-audio" class="c-checkbox__input" name="supp_file_types" value="audio"/><label for="supp_file_types-audio" class="c-checkbox__label">Audio (0)</label></li><li class=""><input type="checkbox" id="supp_file_types-images" class="c-checkbox__input" name="supp_file_types" value="images"/><label for="supp_file_types-images" class="c-checkbox__label">Images (0)</label></li><li class=""><input type="checkbox" id="supp_file_types-zip" class="c-checkbox__input" name="supp_file_types" value="zip"/><label for="supp_file_types-zip" class="c-checkbox__label">Zip (0)</label></li><li class=""><input type="checkbox" id="supp_file_types-other_files" class="c-checkbox__input" name="supp_file_types" value="other files"/><label for="supp_file_types-other_files" class="c-checkbox__label">Other files (0)</label></li></ul></fieldset></details><details class="c-facetbox"><summary class="c-facetbox__summary"><span id="facetbox3">Publication Year</span></summary><fieldset aria-labelledby="facetbox3"><div class="c-pubyear"><div class="c-pubyear__field"><label for="c-pubyear__textfield1">From:</label><input type="text" id="c-pubyear__textfield1" name="pub_year_start" maxLength="4" placeholder="1900" value=""/></div><div class="c-pubyear__field"><label for="c-pubyear__textfield2">To:</label><input type="text" id="c-pubyear__textfield2" name="pub_year_end" maxLength="4" placeholder="2025" value=""/></div><button class="c-pubyear__button">Apply</button></div></fieldset></details><details class="c-facetbox"><summary class="c-facetbox__summary"><span id="facetbox4">Campus</span></summary><fieldset aria-labelledby="facetbox4"><ul class="c-checkbox"><li class=""><input type="checkbox" id="campuses-ucb" class="c-checkbox__input" name="campuses" value="ucb"/><label for="campuses-ucb" class="c-checkbox__label">UC Berkeley (1)</label></li><li class=""><input type="checkbox" id="campuses-ucd" class="c-checkbox__input" name="campuses" value="ucd"/><label for="campuses-ucd" class="c-checkbox__label">UC Davis (1)</label></li><li class=""><input type="checkbox" id="campuses-uci" class="c-checkbox__input" name="campuses" value="uci"/><label for="campuses-uci" class="c-checkbox__label">UC Irvine (0)</label></li><li class=""><input type="checkbox" id="campuses-ucla" class="c-checkbox__input" name="campuses" value="ucla"/><label for="campuses-ucla" class="c-checkbox__label">UCLA (0)</label></li><li class=""><input type="checkbox" id="campuses-ucm" class="c-checkbox__input" name="campuses" value="ucm"/><label for="campuses-ucm" class="c-checkbox__label">UC Merced (0)</label></li><li class=""><input type="checkbox" id="campuses-ucr" class="c-checkbox__input" name="campuses" value="ucr"/><label for="campuses-ucr" class="c-checkbox__label">UC Riverside (0)</label></li><li class=""><input type="checkbox" id="campuses-ucsd" class="c-checkbox__input" name="campuses" value="ucsd"/><label for="campuses-ucsd" class="c-checkbox__label">UC San Diego (0)</label></li><li class=""><input type="checkbox" id="campuses-ucsf" class="c-checkbox__input" name="campuses" value="ucsf"/><label for="campuses-ucsf" class="c-checkbox__label">UCSF (1)</label></li><li class=""><input type="checkbox" id="campuses-ucsb" class="c-checkbox__input" name="campuses" value="ucsb"/><label for="campuses-ucsb" class="c-checkbox__label">UC Santa Barbara (0)</label></li><li class=""><input type="checkbox" id="campuses-ucsc" class="c-checkbox__input" name="campuses" value="ucsc"/><label for="campuses-ucsc" class="c-checkbox__label">UC Santa Cruz (0)</label></li><li class=""><input type="checkbox" id="campuses-ucop" class="c-checkbox__input" name="campuses" value="ucop"/><label for="campuses-ucop" class="c-checkbox__label">UC Office of the President (0)</label></li><li class=""><input type="checkbox" id="campuses-lbnl" class="c-checkbox__input" name="campuses" value="lbnl"/><label for="campuses-lbnl" class="c-checkbox__label">Lawrence Berkeley National Laboratory (1)</label></li><li class=""><input type="checkbox" id="campuses-anrcs" class="c-checkbox__input" name="campuses" value="anrcs"/><label for="campuses-anrcs" class="c-checkbox__label">UC Agriculture & Natural Resources (0)</label></li></ul></fieldset></details><details class="c-facetbox"><summary class="c-facetbox__summary"><span id="facetbox5">Department</span></summary><fieldset aria-labelledby="facetbox5"><ul class="c-checkbox"><li class=""><input type="checkbox" id="departments-lbnl_bs" class="c-checkbox__input" name="departments" value="lbnl_bs"/><label for="departments-lbnl_bs" class="c-checkbox__label">BioSciences (1)</label></li></ul></fieldset></details><details class="c-facetbox"><summary class="c-facetbox__summary"><span id="facetbox6">Journal</span></summary><fieldset aria-labelledby="facetbox6"><ul class="c-checkbox"></ul></fieldset></details><details class="c-facetbox"><summary class="c-facetbox__summary"><span id="facetbox7">Discipline</span></summary><fieldset aria-labelledby="facetbox7"><ul class="c-checkbox"></ul></fieldset></details><details class="c-facetbox"><summary class="c-facetbox__summary"><span id="facetbox8">Reuse License</span></summary><fieldset aria-labelledby="facetbox8"><ul class="c-checkbox"></ul></fieldset></details></div></div><button type="submit" id="facet-form-submit" style="display:none">Search</button></div></aside><main id="maincontent"><section class="o-columnbox1"><header><h2 class="o-columnbox1__heading" aria-live="polite">Scholarly Works (3 results)</h2></header><div class="c-sortpagination"><div class="c-sort"><div class="o-input__droplist1"><label for="c-sort1">Sort By:</label><select name="sort" id="c-sort1" form="facetForm"><option selected="" value="rel">Relevance</option><option value="a-title">A-Z By Title</option><option value="z-title">Z-A By Title</option><option value="a-author">A-Z By Author</option><option value="z-author">Z-A By Author</option><option value="asc">Date Ascending</option><option value="desc">Date Descending</option></select></div></div><input type="hidden" name="start" form="facetForm" value="0"/></div><section class="c-scholworks"><div class="c-scholworks__main-column"><ul class="c-scholworks__tag-list"><li class="c-scholworks__tag-article">Article</li><li class="c-scholworks__tag-peer">Peer Reviewed</li></ul><div><h3 class="c-scholworks__heading"><a href="/uc/item/6h30c60f"><div class="c-clientmarkup">Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS</div></a></h3></div><div class="c-authorlist"><ul class="c-authorlist__list"><li class="c-authorlist__begin"><a href="/search/?q=author%3ADeng%2C%20Kai">Deng, Kai</a>; </li><li><a href="/search/?q=author%3AGuenther%2C%20Joel%20M">Guenther, Joel M</a>; </li><li><a href="/search/?q=author%3AGao%2C%20Jian">Gao, Jian</a>; </li><li><a href="/search/?q=author%3ABowen%2C%20Benjamin%20P">Bowen, Benjamin P</a>; </li><li><a href="/search/?q=author%3ATran%2C%20Huu">Tran, Huu</a>; </li><li><a href="/search/?q=author%3AReyes-Ortiz%2C%20Vimalier">Reyes-Ortiz, Vimalier</a>; </li><li><a href="/search/?q=author%3ACheng%2C%20Xiaoliang">Cheng, Xiaoliang</a>; </li><li><a href="/search/?q=author%3ASathitsuksanoh%2C%20Noppadon">Sathitsuksanoh, Noppadon</a>; </li><li><a href="/search/?q=author%3AHeins%2C%20Richard">Heins, Richard</a>; </li><li><a href="/search/?q=author%3ATakasuka%2C%20Taichi%20E">Takasuka, Taichi E</a>; </li><li><a href="/search/?q=author%3ABergeman%2C%20Lai%20F">Bergeman, Lai F</a>; </li><li><a href="/search/?q=author%3AGeertz-Hansen%2C%20Henrik">Geertz-Hansen, Henrik</a>; </li><li><a href="/search/?q=author%3ADeutsch%2C%20Samuel">Deutsch, Samuel</a>; </li><li><a href="/search/?q=author%3ALoqu%C3%A9%2C%20Dominique">Loqu茅, Dominique</a>; </li><li><a href="/search/?q=author%3ASale%2C%20Kenneth%20L">Sale, Kenneth L</a>; </li><li><a href="/search/?q=author%3ASimmons%2C%20Blake%20A">Simmons, Blake A</a>; </li><li><a href="/search/?q=author%3AAdams%2C%20Paul%20D">Adams, Paul D</a>; </li><li><a href="/search/?q=author%3ASingh%2C%20Anup%20K">Singh, Anup K</a>; </li><li><a href="/search/?q=author%3AFox%2C%20Brian%20G">Fox, Brian G</a>; </li><li class="c-authorlist__end"><a href="/search/?q=author%3ANorthen%2C%20Trent%20R">Northen, Trent R</a> </li></ul></div><div class="c-scholworks__publication"><a href="/uc/ucb_postprints">UC Berkeley Previously Published Works</a> (2015)</div><div class="c-scholworks__abstract"><div class="c-clientmarkup">Cost-effective hydrolysis of biomass into sugars for biofuel production requires high-performance low-cost glycoside hydrolase (GH) cocktails that are active under demanding process conditions. Improving the performance of GH cocktails depends on knowledge of many critical parameters, including individual enzyme stabilities, optimal reaction conditions, kinetics, and specificity of reaction. With this information, rate- and/or yield-limiting reactions can be potentially improved through substitution, synergistic complementation, or protein engineering. Given the wide range of substrates and methods used for GH characterization, it is difficult to compare results across a myriad of approaches to identify high performance and synergistic combinations of enzymes. Here, we describe a platform for systematic screening of GH activities using automatic biomass handling, bioconjugate chemistry, robotic liquid handling, and nanostructure-initiator mass spectrometry (NIMS). Twelve well-characterized substrates spanning the types of glycosidic linkages found in plant cell walls are included in the experimental workflow. To test the application of this platform and substrate panel, we studied the reactivity of three engineered cellulases and their synergy of combination across a range of reaction conditions and enzyme concentrations. We anticipate that large-scale screening using the standardized platform and substrates will generate critical datasets to enable direct comparison of enzyme activities for cocktail design.</div></div><div class="c-scholworks__media"><ul class="c-medialist"></ul></div></div><div class="c-scholworks__ancillary"><a class="c-scholworks__thumbnail" href="/uc/item/6h30c60f"><img src="/cms-assets/19a15fff46fcadf12ba12a685755ea0403d6faa90194547c69f296d6cfc76874" alt="Cover page: Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS"/></a></div></section><section class="c-scholworks"><div class="c-scholworks__main-column"><ul class="c-scholworks__tag-list"><li class="c-scholworks__tag-article">Article</li><li class="c-scholworks__tag-peer">Peer Reviewed</li></ul><div><h3 class="c-scholworks__heading"><a href="/uc/item/8188960d"><div class="c-clientmarkup">Primary immunodeficiency diseases: Genomic approaches delineate heterogeneous Mendelian disorders</div></a></h3></div><div class="c-authorlist"><ul class="c-authorlist__list"><li class="c-authorlist__begin"><a href="/search/?q=author%3AStray-Pedersen%2C%20Asbj%C3%B8rg">Stray-Pedersen, Asbj酶rg</a>; </li><li><a href="/search/?q=author%3ASorte%2C%20Hanne%20S%C3%B8rmo">Sorte, Hanne S酶rmo</a>; </li><li><a href="/search/?q=author%3ASamarakoon%2C%20Pubudu">Samarakoon, Pubudu</a>; </li><li><a href="/search/?q=author%3AGambin%2C%20Tomasz">Gambin, Tomasz</a>; </li><li><a href="/search/?q=author%3AChinn%2C%20Ivan%20K">Chinn, Ivan K</a>; </li><li><a href="/search/?q=author%3AAkdemir%2C%20Zeynep%20H%20Coban">Akdemir, Zeynep H Coban</a>; </li><li><a href="/search/?q=author%3AErichsen%2C%20Hans%20Christian">Erichsen, Hans Christian</a>; </li><li><a href="/search/?q=author%3AForbes%2C%20Lisa%20R">Forbes, Lisa R</a>; </li><li><a href="/search/?q=author%3AGu%2C%20Shen">Gu, Shen</a>; </li><li><a href="/search/?q=author%3AYuan%2C%20Bo">Yuan, Bo</a>; </li><li><a href="/search/?q=author%3AJhangiani%2C%20Shalini%20N">Jhangiani, Shalini N</a>; </li><li><a href="/search/?q=author%3AMuzny%2C%20Donna%20M">Muzny, Donna M</a>; </li><li><a href="/search/?q=author%3AR%C3%B8dningen%2C%20Olaug%20Kristin">R酶dningen, Olaug Kristin</a>; </li><li><a href="/search/?q=author%3ASheng%2C%20Ying">Sheng, Ying</a>; </li><li><a href="/search/?q=author%3ANicholas%2C%20Sarah%20K">Nicholas, Sarah K</a>; </li><li><a href="/search/?q=author%3ANoroski%2C%20Lenora%20M">Noroski, Lenora M</a>; </li><li><a href="/search/?q=author%3ASeeborg%2C%20Filiz%20O">Seeborg, Filiz O</a>; </li><li><a href="/search/?q=author%3ADavis%2C%20Carla%20M">Davis, Carla M</a>; </li><li><a href="/search/?q=author%3ACanter%2C%20Debra%20L">Canter, Debra L</a>; </li><li><a href="/search/?q=author%3AMace%2C%20Emily%20M">Mace, Emily M</a>; </li><li><a href="/search/?q=author%3AVece%2C%20Timothy%20J">Vece, Timothy J</a>; </li><li><a href="/search/?q=author%3AAllen%2C%20Carl%20E">Allen, Carl E</a>; </li><li><a href="/search/?q=author%3AAbhyankar%2C%20Harshal%20A">Abhyankar, Harshal A</a>; </li><li><a href="/search/?q=author%3ABoone%2C%20Philip%20M">Boone, Philip M</a>; </li><li><a href="/search/?q=author%3ABeck%2C%20Christine%20R">Beck, Christine R</a>; </li><li><a href="/search/?q=author%3AWiszniewski%2C%20Wojciech">Wiszniewski, Wojciech</a>; </li><li><a href="/search/?q=author%3AFevang%2C%20B%C3%B8rre">Fevang, B酶rre</a>; </li><li><a href="/search/?q=author%3AAukrust%2C%20P%C3%A5l">Aukrust, P氓l</a>; </li><li><a href="/search/?q=author%3ATj%C3%B8nnfjord%2C%20Geir%20E">Tj酶nnfjord, Geir E</a>; </li><li><a href="/search/?q=author%3AGedde-Dahl%2C%20Tobias">Gedde-Dahl, Tobias</a>; </li><li><a href="/search/?q=author%3AHjorth-Hansen%2C%20Henrik">Hjorth-Hansen, Henrik</a>; </li><li><a href="/search/?q=author%3ADybedal%2C%20Ingunn">Dybedal, Ingunn</a>; </li><li><a href="/search/?q=author%3ANord%C3%B8y%2C%20Ingvild">Nord酶y, Ingvild</a>; </li><li><a href="/search/?q=author%3AJ%C3%B8rgensen%2C%20Silje%20F">J酶rgensen, Silje F</a>; </li><li><a href="/search/?q=author%3AAbrahamsen%2C%20Tore%20G">Abrahamsen, Tore G</a>; </li><li><a href="/search/?q=author%3A%C3%98verland%2C%20Torstein">脴verland, Torstein</a>; </li><li><a href="/search/?q=author%3ABechensteen%2C%20Anne%20Grete">Bechensteen, Anne Grete</a>; </li><li><a href="/search/?q=author%3ASkogen%2C%20Vegard">Skogen, Vegard</a>; </li><li><a href="/search/?q=author%3AOsnes%2C%20Liv%20TN">Osnes, Liv TN</a>; </li><li><a href="/search/?q=author%3AKulseth%2C%20Mari%20Ann">Kulseth, Mari Ann</a>; </li><li><a href="/search/?q=author%3APrescott%2C%20Trine%20E">Prescott, Trine E</a>; </li><li><a href="/search/?q=author%3ARustad%2C%20Cecilie%20F">Rustad, Cecilie F</a>; </li><li><a href="/search/?q=author%3AHeimdal%2C%20Ketil%20R">Heimdal, Ketil R</a>; </li><li><a href="/search/?q=author%3ABelmont%2C%20John%20W">Belmont, John W</a>; </li><li><a href="/search/?q=author%3ARider%2C%20Nicholas%20L">Rider, Nicholas L</a>; </li><li><a href="/search/?q=author%3AChinen%2C%20Javier">Chinen, Javier</a>; </li><li><a href="/search/?q=author%3ACao%2C%20Tram%20N">Cao, Tram N</a>; </li><li><a href="/search/?q=author%3ASmith%2C%20Eric%20A">Smith, Eric A</a>; </li><li><a href="/search/?q=author%3ACaldirola%2C%20Maria%20Soledad">Caldirola, Maria Soledad</a>; </li><li><a href="/search/?q=author%3ABezrodnik%2C%20Liliana">Bezrodnik, Liliana</a>; </li><li><a href="/search/?q=author%3AReyes%2C%20Saul%20Oswaldo%20Lugo">Reyes, Saul Oswaldo Lugo</a>; </li><li><a href="/search/?q=author%3ARosales%2C%20Francisco%20J%20Espinosa">Rosales, Francisco J Espinosa</a>; </li><li><a href="/search/?q=author%3AGuerrero-Cursaru%2C%20Nina%20Denisse">Guerrero-Cursaru, Nina Denisse</a>; </li><li><a href="/search/?q=author%3APedroza%2C%20Luis%20Alberto">Pedroza, Luis Alberto</a>; </li><li><a href="/search/?q=author%3APoli%2C%20Cecilia%20M">Poli, Cecilia M</a>; </li><li><a href="/search/?q=author%3AFranco%2C%20Jose%20L">Franco, Jose L</a>; </li><li><a href="/search/?q=author%3AVargas%2C%20Claudia%20M%20Trujillo">Vargas, Claudia M Trujillo</a>; </li><li><a href="/search/?q=author%3ABecerra%2C%20Juan%20Carlos%20Aldave">Becerra, Juan Carlos Aldave</a>; </li><li><a href="/search/?q=author%3AWright%2C%20Nicola">Wright, Nicola</a>; </li><li><a href="/search/?q=author%3AIssekutz%2C%20Thomas%20B">Issekutz, Thomas B</a>; </li><li><a href="/search/?q=author%3AIssekutz%2C%20Andrew%20C">Issekutz, Andrew C</a>; </li><li><a href="/search/?q=author%3AAbbott%2C%20Jordan">Abbott, Jordan</a>; </li><li><a href="/search/?q=author%3ACaldwell%2C%20Jason%20W">Caldwell, Jason W</a>; </li><li><a href="/search/?q=author%3ABayer%2C%20Diana%20K">Bayer, Diana K</a>; </li><li><a href="/search/?q=author%3AChan%2C%20Alice%20Y">Chan, Alice Y</a>; </li><li><a href="/search/?q=author%3AAiuti%2C%20Alessandro">Aiuti, Alessandro</a>; </li><li><a href="/search/?q=author%3ACancrini%2C%20Caterina">Cancrini, Caterina</a>; </li><li><a href="/search/?q=author%3AHolmberg%2C%20Eva">Holmberg, Eva</a>; </li><li><a href="/search/?q=author%3AWest%2C%20Christina">West, Christina</a>; </li><li><a href="/search/?q=author%3ABurstedt%2C%20Magnus">Burstedt, Magnus</a>; </li><li><a href="/search/?q=author%3AKaraca%2C%20Ender">Karaca, Ender</a>; </li><li><a href="/search/?q=author%3AYesil%2C%20G%C3%B6zde">Yesil, G枚zde</a>; </li><li><a href="/search/?q=author%3AArtac%2C%20Hasibe">Artac, Hasibe</a>; </li><li><a href="/search/?q=author%3ABayram%2C%20Yavuz">Bayram, Yavuz</a>; </li><li><a href="/search/?q=author%3AAtik%2C%20Mehmed%20Musa">Atik, Mehmed Musa</a>; </li><li><a href="/search/?q=author%3AEldomery%2C%20Mohammad%20K">Eldomery, Mohammad K</a>; </li><li><a href="/search/?q=author%3AEhlayel%2C%20Mohammad%20S">Ehlayel, Mohammad S</a>; </li><li><a href="/search/?q=author%3AJolles%2C%20Stephen">Jolles, Stephen</a>; </li><li><a href="/search/?q=author%3AFlat%C3%B8%2C%20Berit">Flat酶, Berit</a>; </li><li><a href="/search/?q=author%3ABertuch%2C%20Alison%20A">Bertuch, Alison A</a>; </li><li><a href="/search/?q=author%3AHanson%2C%20I%20Celine">Hanson, I Celine</a>; </li><li><a href="/search/?q=author%3AZhang%2C%20Victor%20W">Zhang, Victor W</a>; </li><li><a href="/search/?q=author%3AWong%2C%20Lee-Jun">Wong, Lee-Jun</a>; </li><li><a href="/search/?q=author%3AHu%2C%20Jianhong">Hu, Jianhong</a>; </li><li><a href="/search/?q=author%3AWalkiewicz%2C%20Magdalena">Walkiewicz, Magdalena</a>; </li><li><a href="/search/?q=author%3AYang%2C%20Yaping">Yang, Yaping</a>; </li><li><a href="/search/?q=author%3AEng%2C%20Christine%20M">Eng, Christine M</a>; </li><li><a href="/search/?q=author%3ABoerwinkle%2C%20Eric">Boerwinkle, Eric</a>; </li><li><a href="/search/?q=author%3AGibbs%2C%20Richard%20A">Gibbs, Richard A</a>; </li><li><a href="/search/?q=author%3AShearer%2C%20William%20T">Shearer, William T</a>; </li><li><a href="/search/?q=author%3ALyle%2C%20Robert">Lyle, Robert</a>; </li><li><a href="/search/?q=author%3AOrange%2C%20Jordan%20S">Orange, Jordan S</a>; </li><li class="c-authorlist__end"><a href="/search/?q=author%3ALupski%2C%20James%20R">Lupski, James R</a> </li></ul></div><div class="c-scholworks__publication"><a href="/uc/ucsf_postprints">UC San Francisco Previously Published Works</a> (2017)</div><div class="c-scholworks__abstract"><div class="c-clientmarkup"><h3>Background</h3>Primary immunodeficiency diseases (PIDDs) are clinically and genetically heterogeneous disorders thus far associated with mutations in more than 300 genes. The clinical phenotypes derived from distinct genotypes can overlap. Genetic etiology can be a prognostic indicator of disease severity and can influence treatment decisions.<h3>Objective</h3>We sought to investigate the ability of whole-exome screening methods to detect disease-causing variants in patients with PIDDs.<h3>Methods</h3>Patients with PIDDs from 278 families from 22 countries were investigated by using whole-exome sequencing. Computational copy number variant (CNV) prediction pipelines and an exome-tiling chromosomal microarray were also applied to identify intragenic CNVs. Analytic approaches initially focused on 475 known or candidate PIDD genes but were nonexclusive and further tailored based on clinical data, family history, and immunophenotyping.<h3>Results</h3>A likely molecular diagnosis was achieved in 110 (40%) unrelated probands. Clinical diagnosis was revised in about half (60/110) and management was directly altered in nearly a quarter (26/110) of families based on molecular findings. Twelve PIDD-causing CNVs were detected, including 7 smaller than 30 Kb that would not have been detected with conventional diagnostic CNV arrays.<h3>Conclusion</h3>This high-throughput genomic approach enabled detection of disease-related variants in unexpected genes; permitted detection of low-grade constitutional, somatic, and revertant mosaicism; and provided evidence of a mutational burden in mixed PIDD immunophenotypes.</div></div><div class="c-scholworks__media"><ul class="c-medialist"></ul></div></div><div class="c-scholworks__ancillary"><a class="c-scholworks__thumbnail" href="/uc/item/8188960d"><img src="/cms-assets/bc2a1d037867a3c3c4b566c9f2a39e6ce46aa17d213d10a919d90d021bca4446" alt="Cover page: Primary immunodeficiency diseases: Genomic approaches delineate heterogeneous Mendelian disorders"/></a></div></section><section class="c-scholworks"><div class="c-scholworks__main-column"><ul class="c-scholworks__tag-list"><li class="c-scholworks__tag-article">Article</li><li class="c-scholworks__tag-peer">Peer Reviewed</li></ul><div><h3 class="c-scholworks__heading"><a href="/uc/item/0hv6s32x"><div class="c-clientmarkup">The Society for Translational Medicine: clinical practice guidelines for the postoperative management of chest tube for patients undergoing lobectomy.</div></a></h3></div><div class="c-authorlist"><ul class="c-authorlist__list"><li class="c-authorlist__begin"><a href="/search/?q=author%3AGao%2C%20Shugeng">Gao, Shugeng</a>; </li><li><a href="/search/?q=author%3AZhang%2C%20Zhongheng">Zhang, Zhongheng</a>; </li><li><a href="/search/?q=author%3AArag%C3%B3n%2C%20Javier">Arag贸n, Javier</a>; </li><li><a href="/search/?q=author%3ABrunelli%2C%20Alessandro">Brunelli, Alessandro</a>; </li><li><a href="/search/?q=author%3ACassivi%2C%20Stephen">Cassivi, Stephen</a>; </li><li><a href="/search/?q=author%3AChai%2C%20Ying">Chai, Ying</a>; </li><li><a href="/search/?q=author%3AChen%2C%20Chang">Chen, Chang</a>; </li><li><a href="/search/?q=author%3AChen%2C%20Chun">Chen, Chun</a>; </li><li><a href="/search/?q=author%3AChen%2C%20Gang">Chen, Gang</a>; </li><li><a href="/search/?q=author%3AChen%2C%20Haiquan">Chen, Haiquan</a>; </li><li><a href="/search/?q=author%3AChen%2C%20Jin-Shing">Chen, Jin-Shing</a>; </li><li><a href="/search/?q=author%3ACooke%2C%20David">Cooke, David</a>; </li><li><a href="/search/?q=author%3ADowns%2C%20John">Downs, John</a>; </li><li><a href="/search/?q=author%3AFalcoz%2C%20Pierre-Emmanuel">Falcoz, Pierre-Emmanuel</a>; </li><li><a href="/search/?q=author%3AFang%2C%20Wentao">Fang, Wentao</a>; </li><li><a href="/search/?q=author%3AFilosso%2C%20Pier">Filosso, Pier</a>; </li><li><a href="/search/?q=author%3AFu%2C%20Xiangning">Fu, Xiangning</a>; </li><li><a href="/search/?q=author%3AForce%2C%20Seth">Force, Seth</a>; </li><li><a href="/search/?q=author%3AGarutti%2C%20Mart%C3%ADnez">Garutti, Mart铆nez</a>; </li><li><a href="/search/?q=author%3AGonzalez-Rivas%2C%20Diego">Gonzalez-Rivas, Diego</a>; </li><li><a href="/search/?q=author%3AGossot%2C%20Dominique">Gossot, Dominique</a>; </li><li><a href="/search/?q=author%3AHansen%2C%20Henrik">Hansen, Henrik</a>; </li><li><a href="/search/?q=author%3AHe%2C%20Jianxing">He, Jianxing</a>; </li><li><a href="/search/?q=author%3AHe%2C%20Jie">He, Jie</a>; </li><li><a href="/search/?q=author%3AHolbek%2C%20Bo">Holbek, Bo</a>; </li><li><a href="/search/?q=author%3AHu%2C%20Jian">Hu, Jian</a>; </li><li><a href="/search/?q=author%3AHuang%2C%20Yunchao">Huang, Yunchao</a>; </li><li><a href="/search/?q=author%3AIbrahim%2C%20Mohsen">Ibrahim, Mohsen</a>; </li><li><a href="/search/?q=author%3AImperatori%2C%20Andrea">Imperatori, Andrea</a>; </li><li><a href="/search/?q=author%3AIsmail%2C%20Mahmoud">Ismail, Mahmoud</a>; </li><li><a href="/search/?q=author%3AJiang%2C%20Gening">Jiang, Gening</a>; </li><li><a href="/search/?q=author%3AJiang%2C%20Hongjing">Jiang, Hongjing</a>; </li><li><a href="/search/?q=author%3AJiang%2C%20Zhongmin">Jiang, Zhongmin</a>; </li><li><a href="/search/?q=author%3AKim%2C%20Hyun">Kim, Hyun</a>; </li><li><a href="/search/?q=author%3ALi%2C%20Danqing">Li, Danqing</a>; </li><li><a href="/search/?q=author%3ALi%2C%20Gaofeng">Li, Gaofeng</a>; </li><li><a href="/search/?q=author%3ALi%2C%20Hui">Li, Hui</a>; </li><li><a href="/search/?q=author%3ALi%2C%20Qiang">Li, Qiang</a>; </li><li><a href="/search/?q=author%3ALi%2C%20Xiaofei">Li, Xiaofei</a>; </li><li><a href="/search/?q=author%3ALi%2C%20Yin">Li, Yin</a>; </li><li><a href="/search/?q=author%3ALi%2C%20Zhijun">Li, Zhijun</a>; </li><li><a href="/search/?q=author%3ALim%2C%20Eric">Lim, Eric</a>; </li><li><a href="/search/?q=author%3ALiu%2C%20Chia-Chuan">Liu, Chia-Chuan</a>; </li><li><a href="/search/?q=author%3ALiu%2C%20Deruo">Liu, Deruo</a>; </li><li><a href="/search/?q=author%3ALiu%2C%20Lunxu">Liu, Lunxu</a>; </li><li><a href="/search/?q=author%3ALiu%2C%20Yongyi">Liu, Yongyi</a>; </li><li><a href="/search/?q=author%3ALobdell%2C%20Kevin">Lobdell, Kevin</a>; </li><li><a href="/search/?q=author%3AMa%2C%20Haitao">Ma, Haitao</a>; </li><li><a href="/search/?q=author%3AMao%2C%20Weimin">Mao, Weimin</a>; </li><li><a href="/search/?q=author%3AMao%2C%20Yousheng">Mao, Yousheng</a>; </li><li><a href="/search/?q=author%3AMou%2C%20Juwei">Mou, Juwei</a>; </li><li><a href="/search/?q=author%3ANg%2C%20Calvin">Ng, Calvin</a>; </li><li><a href="/search/?q=author%3ANovoa%2C%20Nuria">Novoa, Nuria</a>; </li><li><a href="/search/?q=author%3APetersen%2C%20Ren%C3%A9">Petersen, Ren茅</a>; </li><li><a href="/search/?q=author%3AOizumi%2C%20Hiroyuki">Oizumi, Hiroyuki</a>; </li><li><a href="/search/?q=author%3APapagiannopoulos%2C%20Kostas">Papagiannopoulos, Kostas</a>; </li><li><a href="/search/?q=author%3APompili%2C%20Cecilia">Pompili, Cecilia</a>; </li><li><a href="/search/?q=author%3AQiao%2C%20Guibin">Qiao, Guibin</a>; </li><li><a href="/search/?q=author%3ARefai%2C%20Majed">Refai, Majed</a>; </li><li><a href="/search/?q=author%3ARocco%2C%20Gaetano">Rocco, Gaetano</a>; </li><li><a href="/search/?q=author%3ARuffini%2C%20Erico">Ruffini, Erico</a>; </li><li><a href="/search/?q=author%3ASalati%2C%20Michele">Salati, Michele</a>; </li><li><a href="/search/?q=author%3ASeguin-Givelet%2C%20Agathe">Seguin-Givelet, Agathe</a>; </li><li><a href="/search/?q=author%3ASihoe%2C%20Alan">Sihoe, Alan</a>; </li><li><a href="/search/?q=author%3ATan%2C%20Lijie">Tan, Lijie</a>; </li><li><a href="/search/?q=author%3ATan%2C%20Qunyou">Tan, Qunyou</a>; </li><li><a href="/search/?q=author%3ATong%2C%20Tang">Tong, Tang</a>; </li><li><a href="/search/?q=author%3ATsakiridis%2C%20Kosmas">Tsakiridis, Kosmas</a>; </li><li><a href="/search/?q=author%3AVenuta%2C%20Federico">Venuta, Federico</a>; </li><li><a href="/search/?q=author%3AVeronesi%2C%20Giulia">Veronesi, Giulia</a>; </li><li><a href="/search/?q=author%3AVillamizar%2C%20Nestor">Villamizar, Nestor</a>; </li><li><a href="/search/?q=author%3AWang%2C%20Haidong">Wang, Haidong</a>; </li><li><a href="/search/?q=author%3AWang%2C%20Qun">Wang, Qun</a>; </li><li><a href="/search/?q=author%3AWang%2C%20Ruwen">Wang, Ruwen</a>; </li><li><a href="/search/?q=author%3AWang%2C%20Shumin">Wang, Shumin</a>; </li><li><a href="/search/?q=author%3AWright%2C%20Gavin">Wright, Gavin</a>; </li><li><a href="/search/?q=author%3AXie%2C%20Deyao">Xie, Deyao</a>; </li><li><a href="/search/?q=author%3AXue%2C%20Qi">Xue, Qi</a>; </li><li><a href="/search/?q=author%3AXue%2C%20Tao">Xue, Tao</a>; </li><li><a href="/search/?q=author%3AXu%2C%20Lin">Xu, Lin</a>; </li><li><a href="/search/?q=author%3AXu%2C%20Shidong">Xu, Shidong</a>; </li><li><a href="/search/?q=author%3AXu%2C%20Songtao">Xu, Songtao</a>; </li><li><a href="/search/?q=author%3AYan%2C%20Tiansheng">Yan, Tiansheng</a>; </li><li><a href="/search/?q=author%3AYu%2C%20Fenglei">Yu, Fenglei</a>; </li><li><a href="/search/?q=author%3AYu%2C%20Zhentao">Yu, Zhentao</a>; </li><li><a href="/search/?q=author%3AZhang%2C%20Chunfang">Zhang, Chunfang</a>; </li><li><a href="/search/?q=author%3AZhang%2C%20Lanjun">Zhang, Lanjun</a>; </li><li><a href="/search/?q=author%3AZhang%2C%20Tao">Zhang, Tao</a>; </li><li><a href="/search/?q=author%3AZhang%2C%20Xun">Zhang, Xun</a>; </li><li><a href="/search/?q=author%3AZhao%2C%20Xiaojing">Zhao, Xiaojing</a>; </li><li><a href="/search/?q=author%3AZhao%2C%20Xuewei">Zhao, Xuewei</a>; </li><li><a href="/search/?q=author%3AZhi%2C%20Xiuyi">Zhi, Xiuyi</a>; </li><li class="c-authorlist__end"><a href="/search/?q=author%3AZhou%2C%20Qinghua">Zhou, Qinghua</a> </li></ul></div><div class="c-scholworks__publication"><a href="/uc/ucd_postprints">UC Davis Previously Published Works</a> (2017)</div><div class="c-scholworks__abstract"><div class="c-clientmarkup">The Society for Translational Medicine and The Chinese Society for Thoracic and Cardiovascular Surgery conducted a systematic review of the literature in an attempt to improve our understanding in the postoperative management of chest tubes of patients undergoing pulmonary lobectomy. Recommendations were produced and classified based on an internationally accepted GRADE system. The following recommendations were extracted in the present review: (I) chest tubes can be removed safely with daily pleural fluid of up to 450 mL (non-chylous and non-sanguinous), which may reduce chest tube duration and hospital length of stay (2B); (II) in rare instances, e.g., persistent abundant fluid production, the use of PrRP/B <0.5 when evaluating fluid output to determine chest tube removal might be beneficial (2B); (III) it is recommended that one chest tube is adequate following pulmonary lobectomy, except for hemorrhage and space problems (2A); (IV) chest tube clearance by milking and stripping is not recommended after lung resection (2B); (V) chest tube suction is not necessary for patients undergoing lobectomy after first postoperative day (2A); (VI) regulated chest tube suction [-11 (-1.08 kPa) to -20 (1.96 kPa) cmH2O depending upon the type of lobectomy] is not superior to regulated seal [-2 (0.196 kPa) cmH2O] when electronic drainage systems are used after lobectomy by thoracotomy (2B); (VII) chest tube removal recommended at the end of expiration and may be slightly superior to removal at the end of inspiration (2A); (VIII) electronic drainage systems are recommended in the management of chest tube in patients undergoing lobectomy (2B).</div></div><div class="c-scholworks__media"><ul class="c-medialist"></ul></div></div><div class="c-scholworks__ancillary"><a class="c-scholworks__thumbnail" href="/uc/item/0hv6s32x"><img src="/cms-assets/806489c78f75b02e4dfe8776cf5bdd5f148ed79844aa5c57fdf416e5e409032f" alt="Cover page: The Society for Translational Medicine: clinical practice guidelines for the postoperative management of chest tube for patients undergoing lobectomy."/></a></div></section></section></main></form></div><div><div class="c-toplink"><a href="javascript:window.scrollTo(0, 0)">Top</a></div><footer class="c-footer"><nav class="c-footer__nav"><ul><li><a href="/">Home</a></li><li><a href="/aboutEschol">About eScholarship</a></li><li><a href="/campuses">Campus Sites</a></li><li><a href="/ucoapolicies">UC Open Access Policy</a></li><li><a href="/publishing">eScholarship Publishing</a></li><li><a href="https://www.cdlib.org/about/accessibility.html">Accessibility</a></li><li><a href="/privacypolicy">Privacy Statement</a></li><li><a href="/policies">Site Policies</a></li><li><a href="/terms">Terms of Use</a></li><li><a href="/login"><strong>Admin Login</strong></a></li><li><a href="https://help.escholarship.org"><strong>Help</strong></a></li></ul></nav><div class="c-footer__logo"><a href="/"><img class="c-lazyimage" data-src="/images/logo_footer-eschol.svg" alt="eScholarship, University of California"/></a></div><div class="c-footer__copyright">Powered by the<br/><a href="http://www.cdlib.org">California Digital Library</a><br/>Copyright 漏 2017<br/>The Regents of the University of California</div></footer></div></div></div></div> <script src="/js/vendors~app-bundle-2aefc956e545366a5d4e.js"></script> <script src="/js/app-bundle-3c8ebc2ec05dcc3202fd.js"></script> </body> </html>