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ESS Data Scientist helps shed light on assembly mechanism of hepatitis B virus | ESS
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</div> <main class="site-content" role="main"> <a id="main-content" tabindex="-1"></a> <div id="block-content"> <div class="article"> <div class="article__main"> <div class="row"> <header class="article__main-header col-12 col-md-8 col-xl-9"> <h1 class="article__title">ESS Data Scientist helps shed light on assembly mechanism of hepatitis B virus</h1> <div class="article__created-date"> <time datetime="2020-08-21T12:22:34+02:00" class="date date--primary"><time datetime="2020-08-21T12:22:34+02:00" title="Friday, August 21, 2020 - 12:22">August 21, 2020</time> </time> </div> </header> </div> <div class="row"> <div class="article__main-content col-12 col-md-8 col-xl-9"> <div class="article__image"> <div class="media media-image media-image--article-lead"> <div class="media__content"> <picture> <source srcset="/sites/default/files/styles/article_image/public/images/media/2020-08/hbv_pathway.jpg?h=015added&itok=DAuVZwSZ 1x" media="(min-width: 576px)" type="image/jpeg" width="1367" height="808"/> <img class="image" loading="eager" class="image" src="/sites/default/files/styles/article_image/public/images/media/2020-08/hbv_pathway.jpg?h=015added&itok=DAuVZwSZ" width="1367" height="808" alt="HBV Pathways" /> </picture> </div> <footer class="media__footer"> <div class="caption"> Using combination of molecular modeling and time-resolved SAXS the team discovered that HBV virus assembly proceeds through two major intermediates (green and red structural models) from starting dimer (blue) to the complete capsid (purple). </div> </footer> </div> </div> <div class="article__sections"> <section class="layout-section layout-section--primary layout-section--one-columns"> <div class="layout-section__row"> <section class="section section--text"> <p class="lead">Understanding the assembly mechanism of a virus is critical for the development of antiviral drugs to help combat that virus. Learn more about a collaboration between ESS, Lund University, the University of Copenhagen and the UK's Science & Technology Facility Council's ISIS Neutron & Muon Source to understand the assembly mechanism of hepatitis B virus (HBV).</p> <p>Together with collaborators from <a href="https://www.lunduniversity.lu.se" target="_blank">Lund University</a>, <a href="https://www.ku.dk/english/" target="_blank">University of Copenhagen</a> & <a href="https://www.isis.stfc.ac.uk/Pages/home.aspx" target="_blank">ISIS</a>, data scientist, Wojciech Potrzebowski, based at <a href="https://europeanspallationsource.se/data-management-software-centre">ESS Data Management & Software Centre</a> in Denmark, has developed a method that combines molecular modelling with time-resolved small-angle scattering to investigate the self-assembly process of HBV in the presence and absence of ribonucleic acid (RNA). </p> <p>The understanding of such mechanisms is critical for the development of antiviral drugs, biotechnology, protein engineering and vaccine development. The method was applied to study the HBV virus, but it could be further extended to study other viruses, including the novel coronavirus. </p> <div data-embed-button="media" data-entity-embed-display="view_mode:media.portrait_33" data-entity-type="media" data-entity-uuid="6982ba14-4e0d-4429-82e3-d77392361604" class="align-left embedded-entity embedded-media embedded-media-image embedded-media-image--portrait-33" data-langcode="en" data-entity-embed-display-settings="[]"> <div class="media media-image media-image--portrait-33"> <div class="media__content"> <picture><source srcset="/sites/default/files/styles/portrait/public/images/media/2020-08/wojtek_photo.jpg?itok=0XAWi83W 1x" media="(min-width: 576px)" type="image/jpeg" width="660" height="660"></source><img class="image" loading="eager" src="/sites/default/files/styles/portrait/public/images/media/2020-08/wojtek_photo.jpg?itok=0XAWi83W" width="660" height="660" alt="Wojciech Potrzebowski " /></picture></div> <footer class="media__footer"><div class="caption"><p>Wojciech Potrzebowski, Data Scientist at ESS.</p> </div> <div class="credit"><p>Credit: Wojciech Potrzebowski </p> </div> </footer></div> </div> <p>Wojciech is the first co-author of this article leading data analysis aspects. "Together with Ingemar Andre from LU, I wrote and submitted an original proposal to the Swedish Research Council, <a href="https://www.vr.se/english" target="_blank">Vetenskåpradet</a>, or VR, who funded this project, " he explains, adding that he also established a "collaboration with Lise Arleth's group at University of Copenhagen and they offered to share their beam time at <a href="https://www.esrf.eu" target="_blank">ESRF</a> (The European Synchrotron) with us, so that we could perform our measurements. (Lise is a well-established scientist in the field of small angle scattering and she runs a group at <a href="https://www.nbi.ku.dk/english/" target="_blank">Niels Bohr Institute</a> at University of Copenhagen). </p> <p>"For this study, time-resolved small angle X-ray scattering data was collected at <a href="https://www.esrf.eu">ESRF</a>, which provides sufficient time resolution and quality to observe intermediate structures. It doesn’t, however, provide full insight into RNA/protein composition which can be obtained from experiments using neutrons.</p> <p>"Once ESS is open to scientists, instruments like ESS's <a href="https://europeanspallationsource.se/instruments/loki">LOKI</a>, with its high flux, will open up for such possibilities in the future," concludes Wojciech.</p> </section> <section class="section section--text"> <div class="references">Read the full article 'Assembly of Capsids from Hepatitis B Virus Core Protein Progresses through Highly Populated Intermediates in the Presence and Absence of RNA' here: <br /><strong><a href="https://pubs.acs.org/doi/10.1021/acsnano.0c03569">https://pubs.acs.org/doi/10.1021/acsnano.0c03569</a></strong></div> </section> </div> </section> </div> </div> <aside class="article__sidebar col-4 col-xl-3"> <div class="read-next-block"><div class="read-next-block__inner"> <h4 class="read-next-block__title">Top stories</h4> <div><div 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