Beclin 1 Antibody - BSA Free (NB500-249) by Novus, Part of Bio-Techne

<!DOCTYPE html> <html lang="en" dir="ltr" prefix="og: https://ogp.me/ns#"> <head> <meta charset="utf-8" /> <noscript><style>form.antibot * :not(.antibot-message) { display: none !important; }</style> </noscript><meta name="applicable-device" content="pc,mobile" /> <link rel="canonical" href="https://www.bio-techne.com/p/antibodies/beclin-1-antibody_nb500-249" /> <meta property="og:site_name" content="Bio-Techne" /> <meta property="og:type" content="Website" /> <meta property="og:url" content="https://www.bio-techne.com/" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <script type="application/ld+json">{ "@context": "https://schema.org", "@graph": [ { "@type": "WebPage", "@id": "https://www.bio-techne.com/p/antibodies/beclin-1-antibody_nb500-249", "breadcrumb": { "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Home", "item": "https://www.bio-techne.com/" }, { "@type": "ListItem", "position": 2, "name": "Beclin 1", "item": "https://www.bio-techne.com/t/beclin-1" }, { "@type": "ListItem", "position": 3, "name": "Beclin 1 Antibodies", "item": "https://www.bio-techne.com/t/beclin-1/antibodies" } ] } } ] }</script> <meta http-equiv="x-dns-prefetch-control" content="on" /> <meta http-equiv="x-ua-compatible" content="ie=edge" /> <meta name="keywords" content="" /> <meta name="description" content="Cited in 161 publications. View Beclin 1 Antibody - BSA Free (NB500-249) validated in 3 species & 9 applications from Novus Biologicals." /> <script type="application/ld+json" id="json-ld">[{"@context":"http://schema.org","@type":"Product","additionalProperty":[{"additionalProperty":{"@type":"PropertyValue","name":"Applications","value":"Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot"}},{"additionalProperty":{"@type":"PropertyValue","name":"Immunogen","value":"Internal synthetic peptide to human Beclin 1, within residues 1-100 [UniProt# Q14457]."}}],"aggregateRating":{"@type":"AggregateRating","ratingValue":4,"reviewCount":9,"worstRating":"1","bestRating":"5"},"alternateName":"","brand":{"@type":"Brand","name":"Novus Biologicals"},"description":"Beclin 1 Antibody - BSA Free","image":[{"@type":"ImageObject","url":"https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0018.jpg","description":"Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]"},{"@type":"ImageObject","url":"https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg","description":"Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249]"},{"@type":"ImageObject","url":"https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-NB500-249-img0020.jpg","description":"Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249]"},{"@type":"ImageObject","url":"https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0024.jpg","description":"Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]"},{"@type":"ImageObject","url":"https://resources.bio-techne.com/images/products/Beclin 1 Antibody - BSA Free-Western Blot-NB500-249-img0025.jpg","description":"Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Representative Western blot images and quantitative analyses of Beclin 1 from the left hemisphere of rat brains at different time points after SAH. 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Would you please send me the sequence information, and any data you may have for this peptide?","acceptedAnswer":{"@type":"Answer","text":"In regards to your inquiry, this Beclin 1 peptide is useful as a blocking peptide to our primary antibody NB500-249. This peptide contains amino acids that fall within the range of 1-100. Unfortunately the exact sequence is deemed proprietary by the lab."}},{"@context":"http://schema.org","@type":"Question","name":"I'm looking for a Beclin 1 antibody, which do you recommend for use in western blot?\r\n","acceptedAnswer":{"@type":"Answer","text":"Our best selling beclin 1 antibody NB500-249, has been cited in 41 publications where it was used in western blot. Additionally this beclin 1 antibody is typically available for immediate shipment.\r\n"}},{"@context":"http://schema.org","@type":"Question","name":"I've had good results with your beclin antibody in IHC but now I need a beclin one antibody to use in ELISA, will this beclin antibody also work as a capture antibody in ELISA?\r\n","acceptedAnswer":{"@type":"Answer","text":"This particular beclin 1 antibody has not yet been validated for use in ELISA, we do however have 2 beclin 1 antibodies that are validated in ELISA, NBP2-22176 and NBP1-76648. Alternatively you could take advantage of our innovator's reward program which would allow you to test this beclin 1 antibody in ELISA with out financial risk. Just purchase the beclin antibody and test in ELISA, share your results (negative or positive) and we will issue a voucher for the purchase price of the antibody.\r\n"}},{"@context":"http://schema.org","@type":"Question","name":"Is your belcin 1 antibody NB500-249 available as an atto 488 direct conjugate?\r\n","acceptedAnswer":{"@type":"Answer","text":"Unfortunately we do not offer Atto conjugates but we do offer this beclin 1 antibody as an AlexaFlour 488 direct conjugate and as a DyLight 488 conjugated antibody.\r\n"}},{"@context":"http://schema.org","@type":"Question","name":"Can a Lightning-Link HRP labeling kit be used to label the beclin 1 antibody NB500-249, I've used it to label mouse isotypes but wasn't sure if it will work on a rabbit IgG antibody like this beclin antibody?\r\n","acceptedAnswer":{"@type":"Answer","text":"I can't find any specific mention of this beclin 1 antibody being labeled with a lightning link kit; however lightning link kits are extreamly versatile in terms of labeling ability and should be able to label this beclin 1 antibody without any trouble.\r\n"}},{"@context":"http://schema.org","@type":"Question","name":"I'm using the ATDC5 murine carcinoma cell line, has beclin 1 antibody been documented as working in this cell line?\r\n","acceptedAnswer":{"@type":"Answer","text":"From a cursory look through our publication list I don't see that this beclin 1 antibody has been published as being used in that cell line. We show this beclin 1 antibody was published 19 times as being used in mouse, if I can get your contact info I will take a more in depth look to see if the beclin 1 antibody has been used in that cell line.\r\n"}},{"@context":"http://schema.org","@type":"Question","name":"I'm doning an autophagy study using CHO-K1 cells, do you know if this beclin 1 antibody will detect beclin in that cell line?\r\n","acceptedAnswer":{"@type":"Answer","text":"Based on tissue expression data from protein atlas beclin 1 is not highly expressed in ovarian tissue; however in ovarian cancer tissues beclin 1 expression is much greater. We don't have any beclin 1 antibodies that have been validated for use in hamster so you may want to take advantage of our innovator's reward program.\r\n"}},{"@context":"http://schema.org","@type":"Question","name":"The beclin 1 antibody NB500-249 has good data but I need a beclin atibody that will work with bovine tissue but I don't see bovine listed as reactive with the beclin 1 antibody. Is bovine not listed because you know this beclin one antibody will not recognize?\r\n","acceptedAnswer":{"@type":"Answer","text":"Although reactivity with bovine has not been experimentally validated, a comparison of the human and bovine sequences within the immunogen range indicates that cross reactivity is likely. An alignment of the human beclin 1 sequence and bovine beclin 1 sequence shows there is 95% match in the region that was used as the immunogen.\r\n"}},{"@context":"http://schema.org","@type":"Question","name":"Will this beclin 1 antibody detect beclin that is cleaved at the last 50 aa of the c-terminal?\r\n","acceptedAnswer":{"@type":"Answer","text":"Because the immunogen used to make this beclin 1 antibody falls within the first 100 amino acids of the N-terminal it should detect beclin 1 that is cleaved at the c-terminal.\r\n"}}]}]</script> <link rel="alternate" hreflang="en" href="https://www.bio-techne.com/p/antibodies/beclin-1-antibody_nb500-249" /> <link rel="alternate" hreflang="zh-hans" href="https://www.bio-techne.com/cn/p/antibodies/beclin-1-antibody_nb500-249" /> <link rel="icon" href="/themes/custom/bio_techne_global/favicon.ico" type="image/vnd.microsoft.icon" /> <link rel="preconnect" href="https://resources.bio-techne.com" /> <link rel="preconnect" 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<div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main-onedate-product-pages" class="bg-white px-4 "> <div class="container"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> Products </li> <li> <a href="/t/beclin-1">Beclin 1</a> </li> <li> <a href="/t/beclin-1/antibodies">Beclin 1 Antibodies</a> </li> <li> Beclin 1 Antibody - BSA Free (NB500-249) </li> </ol> </nav> </div> </div> </div> <div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <div id="product-page-details" data-template_in_use="antibody_template" data-time_total="1.8572452068329" data-time_to_compile="0.17679810523987" data-time_to_build="0.15677499771118" data-time_datapoints="1.5236721038818" data-time_template="-1.5236721038818" data-time_post_epcc="0.33357310295105" data-time_epcc="1.5236721038818" data-render_time="Friday 22nd of November 2024 10:53:14 AM" data-fallback="FALSE" data-time_to_process_data="0.17861700057983" class="px-4 container" class="container"> <div id="above-fold-section"> <div class="mb-2 o__product-summary"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="validation-badges-header-wrapper"> <a class="knockout-knockdown mr-3 d-inline-flex mt-1" href="/reagents/antibodies/antibody-validation" target="_blank" title="Knockout/Knockdown"></a> </div> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Beclin 1 Antibody - BSA Free </h1> <h2 class="font-weight-normal h4"> Novus Biologicals, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">NB500-249</span> </span> </h2> <div 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COA / SDS / Protocols & Manuals</a> </article> </div> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" alt="Novus Biologicals, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> <div class="row border-top mb-2" id="images-orderdetail-wrapper"> <div class="col-xl-6 mt-2" id="images-wrapper" data-section="image_summary"> <div id="image-tree" data-section="component-product-image-tree" class="vertical lightbox" data-mdb-zoom-effect="true"> <div id="product-image-gallery" class="row"> <div class="col-md-2 d-none d-md-block"> <ul class="list-inline m-0"> <li data-id="1" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg" alt="Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249]" title="Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red)." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg" data-id="0" data-application="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg" class="lazy" /> </div> </li> <li data-id="2" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-NB500-249-img0020.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-NB500-249-img0020.jpg" alt="Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249] - Detection of Beclin 1 (red) in Pheochromocytes of the Adrenal Medulla 40x." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-NB500-249-img0020.jpg" data-id="1" data-application="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-NB500-249-img0020.jpg" class="lazy" /> </div> </li> <li data-id="3" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0024.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0024.jpg" alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on U-87MG cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher)." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0024.jpg" data-id="2" data-application="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0024.jpg" class="lazy" /> </div> </li> <li data-id="4" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin 1 Antibody - BSA Free-Western Blot-NB500-249-img0025.jpg" data-alt="" title="" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Representative Western blot images and quantitative analyses of Beclin 1 from the left hemisphere of rat brains at different time points after SAH. Sample size is 36, n = 6 per group. Data were presented as mean +/- SD. F = 12.37 for Beclin 1. *P < .05, ** P < .01, ***P < .001 vs Sham group. SAH, subarachnoid hemorrhage. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31436915/) licensed under a CC-BY license." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="5" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0011.jpg" data-alt="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" title="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Analysis of Belclin1. Lane 1: human brain. Lane 2: mouse brain." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="6" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB500-249-img0014.jpg" data-alt="Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249]" title="Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Analysis of Beclin1 in mouse kidney. Image courtsey of product review submitted by Kelly Hudkins." data-badges="" data-application="Immunohistochemistry-Paraffin" class="none"></i> </li> <li data-id="7" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-Frozen-NB500-249-img0015.jpg" data-alt="Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249]" title="Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249] - Merged immunostaining of frozen section of Rat brain tissue. Image from verified customer review." data-badges="" data-application="Immunohistochemistry-Frozen" class="none"></i> </li> <li data-id="8" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0016.jpg" data-alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on HeLa cells with NB500-249AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700." data-badges="" data-application="Flow Cytometry" class="none"></i> </li> <li data-id="9" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0021.jpg" data-alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on HepG2 cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher)." data-badges="" data-application="Flow Cytometry" class="none"></i> </li> <li data-id="10" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0022.jpg" data-alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on THP-1 cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher)." data-badges="" data-application="Flow Cytometry" class="none"></i> </li> <li data-id="11" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0023.jpg" data-alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on Neuro2a cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher)." data-badges="" data-application="Flow Cytometry" class="none"></i> </li> <li data-id="12" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Simple-Western-NB500-249-img0012.jpg" data-alt="Simple Western: Beclin 1 AntibodyBSA Free [NB500-249]" title="Simple Western: Beclin 1 AntibodyBSA Free [NB500-249]" data-caption="Simple Western: Beclin 1 Antibody - BSA Free [NB500-249] - Image shows a specific band for Beclin1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" class="none"></i> </li> <li data-id="13" class="none"> <i data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Knockdown-Validated-NB500-249-img0019.jpg" data-alt="Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249]" title="Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249] - siRNA knockdown of becn1 (Beclin 1). Lysates from OKP7 cells treated with non-targeting siRNA (non-target) or an siRNA pool directed against Beclin 1 (becn1) were analyzed by Western blot for Beclin 1 production. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1003394) licensed under a CC-BY license." data-badges="<span class="knockout-validation"></span>" data-application="Knockdown Validated" class="none"></i> </li> <li data-id="14" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-210202423454813.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a fluorescent reporter plasmid to monitor autophagic flux at 8 h following the indicated treatments. GFP (green) & GFP + mRFP (yellow) fluorescence are observed prior to the fusion of autophagosomes with lysosomes whereas only mRFP (red) fluorescence is present in post-fusion autolysosomes. DIC, differential interference contrast microscopy image. DAPI (blue) staining indicates cell nuclei. (B) Quantification of autolysosomes (red puncta) from (A). F(3,13) = 8.756, p = 0.0019; ∗p < 0.05 when compared to all other groups. (C) Western blotting analysis of the indicated autophagy associated protein levels at 24 h following the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Quantification of dendrite beading from (A). F(3,77) = 6.429, p = 0.0006; ∗p < 0.05 when compared to control cells. Error bars show the SEM. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="15" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-2102024234681.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Metabolic profiling & biochemical assay. (A) Relative abundance of the substrates in the glycolytic pathway & TCA cycle in ORP compared to OCL by targeted metabolic profiling. When compared with OCL heart, ORP hearts have significantly lower glucose-6-phosphate & fructose-6-phosphate (both are glycolytic metabolites), & significantly higher a-ketoglutarate, fumarate, malate, & citrate (all are TCA cycle metabolites). *P < 0.05 compared with OCL. See Table S6 for numerical data. (B) A schematic diagram summarizing the changes in metabolism by rapamycin in old heart. (C) Western blots of autophagic markers show no significant change of LC3 II/I, p62, or beclin-1 in cardiac aging. However, OCR has significantly lower p62 than that in OCL. #P < 0.05 compared with OCL. (D) Both CR & RP significantly reduce the age-dependent increase in protein carbonyls (nmol mL−1). #P < 0.05 compared with OCL. (E). Both CR & RP significantly reduce the age-dependent increase in protein ubiquitination.*P < 0.05 compared with YCL & #P < 0.05 compared with OCL. n = 3–8. G6P: glucose 6-phosphate; G1P: glucose 1-phosphate; F6P: fructose 6-phosphate; F1P: fructose 1-phosphate; F16BP: fructose 1,6-bisphosphate; F26BP: fructose 2,6-biphosphate; G3P: glyceraldehyde 3-phosphate; DHAP:dihydroxyacetone phosphate; 2(3)-PGA: 2- or 3-phosphoglycerate; & PEP: phosphoenolpyruvate. Isomers of same molecular weight, that is, G6P versus G1P, F6P versus F1P, & F16BP versus F26BP, were not distinguishable by the LC-MS/MS-based metabolic profiling method. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24612461), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="16" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-210202423454858.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Autophagy associated protein immunoreactivity in HIV-infected brain tissue. (A) Representative images from five randomly selected fields of cells each examined in duplicate frontal lobe white matter sections for the indicated subject groups. The indicated proteins were labeled red & microglia with the cell-type-specific marker Iba1 (green). Blue staining indicates cell nuclei. Arrow heads indicate examples of higher Iba1 immunoreactivity whereas arrows indicate more focal (punctal) vs. diffuse (filamentous) patterns of autophagy associated protein expression. Scale bar = 10 μm. (B) Quantification of relative Iba1 immunoreactivity from (A). F(3,20) = 6.450, p = 0.0031; ∗p < 0.05 when compared to all other subject groups. Error bars show the SEM for the average values of 2–6 regions from each subject group across the six autophagy associated proteins examined. (C) Quantification of the indicated autophagy associated protein relative immunoreactivity from (A). Beclin 1: F(3,12) = 11.29, p = 0.0008; LC3B: F(3,12) = 1.994, p = 0.1687; APG7/ATG7: F(3,12) = 84.20, p = < 0.0001; ATG5: F(3,12) = 6.218, p = 0.0086; p62/SQSTM1: F(3,12) = 87.04, p = < 0.0001; LAMP1: F(3,12) = 8.317, p = 0.0029. ∗p < 0.05 when compared to HIV-negative; #p < 0.05 when compared to HIV-positive; & Ωp < 0.05 when compared to HIV-positive/NCI subjects. Error bars show the SEM for four regions from each subject group. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" class="none"></i> </li> <li data-id="17" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415304271.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Excessive activation of P62-mediated autophagic degradation by the phosphorylated ER alpha-ERK cascades may lead to the autophagic cell death induced by gemcitabine in ER positive MCF-7 cells. B. MCF-7 cells treated by gemcitabine, gemcitabine+ PD98059 (30 μmol/L, added before gemcitabine treatment for 1 h) or DMSO for 48 h. Then total cell lysates subjected to immunoblot analysis with indicated antibodies. The data represented a typical experiment conducted 3times with similar results. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.10363), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="18" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241537194.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin failed to suppress oxidative stress & activate autophagy in Nrf2−/− mice following TBI.Fucoxanthin treatment had no effect on change the level of MDA & the activity of GPx (A) & the expression of Beclin-1, LC3 & p62 (B) in Nrf2−/− mice compared to the vehicle-treated group. n = 6 per group. @p > 0.05 versus TBI + vehicle group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="19" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415291949.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - AMPK knockout interrupts & decreases apoptosis in cochlea. (C) WB results show changes in autophagy-related proteins in cochleae of aging mice. There is a remarkable decline of mTOR signaling (Tg-B1 vs. AMPK+/−/Tg-B1, p<0.0001; Tg-B1 vs. WT, p=0.0001) & more Beclin-1 (Tg-B1 vs. AMPK+/−/Tg-B1, p<0.0001, one-way ANOVA followed by Bonferroni post-test) expressed in cochleae of Tg-B1 mice. (D) The histograms of WB analyses show knockouts of AMPK relieve the ROS-induced autophagic stress in Tg-B1 mice. Analysis performed by using Image J software & one-way ANOVA followed by Bonferroni post-test. * P<0.05, ** P<0.01, ***P<0.001, **** P<0.0001; n=3 per group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32240104), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="20" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415293314.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Baclofen reversed the changes of protein markers characteristic for autophagy in hippocampal CA1 area under chronic cerebral hypoperfusion.(a–c) Five weeks after induction of hypoperfusion, p-mTOR was significantly decreased, & LC3-II, Beclin 1, atg5 & atg7 were significantly increased, & baclofen could reverse the changes of these proteins expression. Treatment with baclofen at 12.5 mg/kg & 25 mg/kg in sham-operated rats did not change the expression of LC3-II, mTOR, p-mTOR, Beclin 1, atg5 & atg7 compared with sham-operated rats (n = 4 in each group). Blots shown have been cropped to fit space requirements & run under the same experimental conditions. *P < 0.05 & **P < 0.01 vs sham-operated rats; ##P < 0.01 vs 2VO rats. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep14474), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="21" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416161847.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin protected primary cultured neurons from TBI.(A) Primary cortical neurons were subjected to scratch injury & then treated with 5, 10 or 20 μM fucoxanthin or DMSO for 1 day. The LDH release assay was used to evaluate cell viability. The percentage of survival cells significantly decreased after TBI compared to the control group. Fucoxanthin treatment significantly increased survival cells after TBI. (B) Fucoxanthin repressed the production of ROS in primary cultured cells after TBI. Cells were subjected to scratch injury & subsequently treated with 100 μM edaravone or 5, 10 or 20 μM fucoxanthin or DMSO for 1 day. Then cells were incubated with DCFH-DA & subjected to fluorescence spectrophotometer analysis. The intracellular ROS was significantly increased after TBI compared to the sham group, & administration of edaravone or fucoxanthin significantly repressed ROS production as compared to the TBI + DMSO group. (C) Fucoxanthin inhibited apoptosis & activated autophagy in primary cultured neurons. Primary cortical neurons were subjected to scratch injury & then treated with 5, 10 or 20 μM fucoxanthin or DMSO for 1 day, the expression of cleaved caspase-3, Beclin-1, LC3 & p62 was measured by western blot. Fucoxanthin significantly decreased the expression of cleaved caspase-3 & p62 while increased the expression of Beclin-1 & LC3-II. Data are presented as mean ± SEM, n = 6 per group; *p < 0.05, **p < 0.01, ***p < 0.001 versus control group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus TBI + DMSO group; @p > 0.05 versus TBI + DMSO group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="22" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416175220.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Inhibition of lysosomes leads to G1 arrest by inactivating cyclin E/CDK2 complex. (A) CQ treatment (10, 50, or 100 μM, 24 h) leads to cell cycle G1 arrest. Quantitation of different cell cycle stages in TM3 cells in the presence or absence of CQ. (B–D) EdU incorporation & mitotic index are reduced in CQ-treated TM3 cells. (B) Immunostaining of EdU (red) & DAPI (blue) in scramble control (CTL) or CQ treated TM3 cells. Quantitation of EdU incorporation (C) or mitotic index (D) in scramble control (CTL) or CQ-treated TM3 cells. These results are mean +/− SD from three independent experiments; more than 1000 cells were counted in each individual group. (E–G) CQ inhibited cyclin E1 expression & CDK2 activation. (E,F) Whole cell extracts of CQ-treated TM3 cells at the concentration of 10, 50, or 100 μM are analyzed by immunoblot with antibodies against Beclin1, cyclin D, cyclin A, cyclin E1, CDK2, phosphorylated CDK2 at Thr160 (pCDK2) & alpha-tubulin. (G) Quantitation of relative intensity of cyclin E & pCDK2 in (F). *P < 0.05; **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-00393-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="23" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241618261.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - 8-Cl-Ado-induces autophagic cell killing. (A) Western blot analysis of beclin1 & ATG7 levels in MCF-7 cells transfected with either a pool of control siRNA (siCONT), siRNA targeting the expression of the beclin1 gene (siBECN1), or targeting the expression of the ATG7 gene (siATG7). Immunoblot analysis of LC3B lipidation & PARP cleavage were assessed as markers of autophagosome formation & apoptosis, respectively. GAPDH was used as loading control. Flow cytometric analysis of cells transfected with siCONT, solid bars, siBECN1, hatched bars, or siATG7, checkered bars, treated with 10 μM 8-Cl-Ado & stained with (B) annexin V & PI, as well as (C) acridine orange. Effect of autophagy on 8-Cl-Ado-inhibiton of clonogenic survival. Cells transfected with (D) siCONT, ○, or siBECN1, ●, & with (E) siCONT, ○, or siATG7, ●, were treated with the indicated doses of 8-Cl-Ado for 3 days, washed with PBS, & cultured in fresh medium for 10 days. Colonies of >50 cells were counted under a dissecting microscope. Image collected & cropped by CiteAb from the following publication (https://jhoonline.biomedcentral.com/articles/10.1186/1756-8722-7-23), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="24" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416171472.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin activated autophagy after TBI.(A) Representative images of immunofluorescence for LC3 surrounding the injured cortex. LC3 punctate dots were observed in the cytoplasm by immunofluorescent staining of LC3 (red). Neuron cells & nuclei are labeled with NeuN (green) & DAPI (blue), respectively. Magnification: 40 x. Scale bar: 50 mm. (B) Mice brain tissues were collected 1 day after TBI in different groups, & the expression of LC3, Beclin-1 & p62 was measured by western blot. Fucoxanthin treatment significantly increased the level of LC3-II & Beclin-1 while decreasing the level of p62 after TBI. (C) 3-MA (400 nM) was injected i.c.v. 30 min before TBI. Mice were then subjected to TBI & treatment of fucoxanthin 30 min after TBI. Pretreatment with 3-MA significantly attenuated fucoxanthin-induced activation of autophagy & suppression of apoptosis & oxidative stress in the ipsilateral cortex. Data are presented as mean ± SEM, n = 6 per group; **p < 0.01, ***p < 0.001 versus sham group; #p < 0.05, ##p < 0.01 versus TBI + vehicle group; &&p < 0.01, &&&p < 0.001 versus TBI + fucoxanthin group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="25" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416165618.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - GABARAP interacts with AC3 via LIRs of AC3 in a ciliary expression‐dependent manner. A‐E) WB (A) & densitometric quantification of the expression of GABARAP (B), ATG5 (C), VPS34 (D), & Beclin1(E) in the hypothalami of AC3+/+, AC3−/−, & hAC3 mice (n = 3 mice per group). Actin served as the loading control. F) Representative IF co‐staining with AC3 & GABARAP antibodies in the VMHs of WT mice. F′) A higher magnification of the boxed region. Scale bars: F) 20 µm; F′) 5 µm. G) Representative images showing the expression levels of GABARAP & AC3 in the VMHs of VMH pIFT88‐AC3 KD mice & the controls. Scale bars: 20 µm. H) Schematic representation of GABARAP binding LIRs at aa488‐aa493 & aa958‐aa963 of AC3. I) Co‐IP analysis of GABARAP & AC3 (WT), AC3 (LIR1 Mut), or AC3 (LIR2 Mut). LgG served as the negative control. J) Pull‐down analysis of GABARAP & AC3. K) Co‐IP analysis of GABARAP & AC3 (WT), AC3 (296 Mut), or AC3 (465 Mut). LgG served as the negative control. L) Schematic representation of AC3 regulating GABARAP. Data represent the mean ± SEM; *p < 0.05 & **p < 0.01; one‐way ANOVA & Bonferroni pairwise comparisons. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34783461), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li data-id="26" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241616566.jpg" data-alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - EMPA treatment enhanced autophagy in failing hearts. (A) Transmission electron microscopy (TEM) showed autophagosomes in the hearts. Red arrows point to autophagosomes. (B) EMPA activated autophagy pathway by inhibiting mTOR pathway. (C) RT-PCR results showed that the mRNA expression of Beclin1, Atg7 & LC3 were increased by EMPA treatment after TAC. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; AMPK, AMP-activated protein kinase; mTOR, mammalian target of rapamycin; Ulk, Unc-51 like autophagy activating kinase; Atg7, autophagy related 7; LC3, light chain 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" class="none"></i> </li> <li class="list-inline-item"> <div class="image-slider-container"> <div class="lightbox product-image-lightbox"> <div class="lightbox border text-bt-dark-blue text-center search_result_thumbnail_image bg-light-gray rounded" style="padding: 20px 10px" title="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Analysis of Belclin1. Lane 1: human brain. Lane 2: mouse brain." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0011.jpg" data-id="3" data-application="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0011.jpg"> +23 <br>images </div> </div> </div> </li> </ul> </div> <div class="col-md-10 border rounded d-flex align-items-center justify-content-center product-image-lightbox"> <div class="text-center my-2" data-brand="novus"> <img src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0018.jpg" alt="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" title="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license." data-badges='' data-id="0" width="250" height="250" fetchpriority="high" as="image" class="search_result_thumbnail_image active" /> </div> </div> </div> </div> </div> <div class="col-xl-6 mt-2" id="orderdetails-wrapper" data-section="order_detail_block"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="NB500-249"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price 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m-0">NB500-249SS</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <div class="atc-control ProductCart-control text-right"> <div class="row container d-flex justify-content-end mx-0 mb-3 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="" class="atc-print-quote-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100">Print Quote</a> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=NB500-249" class="atc-bulk-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> <div class="atc-container order_detail_wrapper conjugates-and-formulations-wrapper my-2 position-relative"> <div class="button-group text-right pl-2"> <a href="#conjugates-NB500-249" class="btn btn-secondary atc-view-pricing atc-view-pricing-products collapsed text-nowrap mb-2" data-id="conjugates-NB500-249" data-toggle="collapse" aria-expanded="false" aria-controls="conjugates">View Available Conjugates </a> </div> <div id="conjugates-NB500-249" class="conjugates-and-formulations-container collapse position-absolute w-100"> <div class="atc-container border rounded bg-white shadow p-2 mt-1"> <div class="commerce-atc-datasheet atc-block-collapsible" data-type="conjugates_and_formulations" data-blockid="nb500-249-conjugate-atc-block"> <div id="nb500-249-conjugate-atc-block" class="atc-lines"> <div class="atc-header row pb-1 m-0"> <div class="atc-head col-4 p-0 font-weight-bold d-sm-none d-md-block">Conjugate</div> <div class="atc-head col-4 p-0 font-weight-bold">Catalog #</div> <div class="atc-head col-4"></div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249AF350" data-titleoverwrite="Alexa Fluor 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af350">Alexa Fluor 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af350">NB500-249AF350</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249af350" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249AF405" data-titleoverwrite="Alexa Fluor 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af405">Alexa Fluor 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af405">NB500-249AF405</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249af405" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249AF488" data-titleoverwrite="Alexa Fluor 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af488">Alexa Fluor 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af488">NB500-249AF488</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249af488" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249AF532" data-titleoverwrite="Alexa Fluor 532"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af532">Alexa Fluor 532</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af532">NB500-249AF532</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249af532" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249AF594" data-titleoverwrite="Alexa Fluor 594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af594">Alexa Fluor 594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af594">NB500-249AF594</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249af594" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249AF647" data-titleoverwrite="Alexa Fluor 647"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af647">Alexa Fluor 647</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af647">NB500-249AF647</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249af647" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249AF700" data-titleoverwrite="Alexa Fluor 700"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af700">Alexa Fluor 700</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af700">NB500-249AF700</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249af700" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249AF750" data-titleoverwrite="Alexa Fluor 750"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af750">Alexa Fluor 750</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249af750">NB500-249AF750</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249af750" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249APC" data-titleoverwrite="Allophycocyanin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249apc">Allophycocyanin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249apc">NB500-249APC</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249apc" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249B" data-titleoverwrite="Biotin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249b">Biotin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249b">NB500-249B</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249b" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249CL1" data-titleoverwrite="CoraFluor 1"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249cl1">CoraFluor 1</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249cl1">NB500-249CL1</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249cl1" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249UV" data-titleoverwrite="DyLight 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249uv">DyLight 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249uv">NB500-249UV</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249uv" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249V" data-titleoverwrite="DyLight 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249v">DyLight 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249v">NB500-249V</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249v" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249G" data-titleoverwrite="DyLight 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249g">DyLight 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249g">NB500-249G</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249g" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249R" data-titleoverwrite="DyLight 550"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249r">DyLight 550</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249r">NB500-249R</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249r" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB500-249DL594" data-titleoverwrite="DyLight 594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beclin-1-antibody_nb500-249dl594">DyLight 594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beclin-1-antibody_nb500-249dl594">NB500-249DL594</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beclin-1-antibody_nb500-249dl594" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div 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class="d-block">Validated:</h4> <div class="d-block pb-2"> Human, Mouse, Rat </div> <h4 class="d-block">Cited:</h4> <div class="d-block pb-2"> Human, Mouse, Rat, Bovine, SARS-CoV </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Applications</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <h4 class="d-block">Validated:</h4> <div class="d-block pb-2"> Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot </div> <h4 class="d-block">Cited:</h4> <div class="d-block pb-2"> Block/Neutralize, IF/IHC, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Label</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Unconjugated </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Antibody Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Polyclonal Rabbit IgG </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Format</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> BSA Free </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Concentration</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 1.0 mg/ml </div> </div> </div> <div class="container"> <div class="row product-data"> <div class="col-lg-6 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4><a href="/t/beclin-1/antibodies">View all Beclin 1 Antibodies »</a></h4> </div> </div> </div> </div> <div class="border-top border-bottom recommended-products-section" id="recommendation-viewed" data-product_code="NB500-249" data-type=viewed></div> <div id="product-specifications" class="bt-pp-border-bottom py-5" data-section="section-base--product-specifications"> <h3>Product Specifications</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Immunogen</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Internal synthetic peptide to human Beclin 1, within residues 1-100 [UniProt# Q14457]. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Localization</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Clonality</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Polyclonal </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Host</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Rabbit </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Isotype</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> IgG </div> </div> </div> </div> <div id="scientific-data" class="bt-pp-border-bottom py-5" data-anchor-label="Scientific Data" data-section="section-base--scientific-data"> <div> <h3>Scientific Data Images for Beclin 1 Antibody - BSA Free </h3> </div> <div class="product-data-wrapper mt-5" id="scientific-data-table" data-scientific-count="24"> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0018.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0018.jpg" alt="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" title="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license. </div> </div> </div> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg" alt="Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249]" title="Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red)." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). </div> </div> </div> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-NB500-249-img0020.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-NB500-249-img0020.jpg" alt="Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249] - Detection of Beclin 1 (red) in Pheochromocytes of the Adrenal Medulla 40x." data-badges="" data-application="Immunohistochemistry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249] - Detection of Beclin 1 (red) in Pheochromocytes of the Adrenal Medulla 40x. </div> </div> </div> <span class="product-data-wrapper table" id="more-scientific-data" style="display:none"> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0024.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0024.jpg" alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on U-87MG cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher)." data-badges="" data-application="Flow Cytometry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on U-87MG cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher). </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin 1 Antibody - BSA Free-Western Blot-NB500-249-img0025.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin 1 Antibody - BSA Free-Western Blot-NB500-249-img0025.jpg" alt="" title="" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Representative Western blot images and quantitative analyses of Beclin 1 from the left hemisphere of rat brains at different time points after SAH. Sample size is 36, n = 6 per group. Data were presented as mean +/- SD. F = 12.37 for Beclin 1. *P < .05, ** P < .01, ***P < .001 vs Sham group. SAH, subarachnoid hemorrhage. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31436915/) licensed under a CC-BY license." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Representative Western blot images and quantitative analyses of Beclin 1 from the left hemisphere of rat brains at different time points after SAH. Sample size is 36, n = 6 per group. Data were presented as mean +/- SD. F = 12.37 for Beclin 1. *P < .05, ** P < .01, ***P < .001 vs Sham group. SAH, subarachnoid hemorrhage. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31436915/) licensed under a CC-BY license. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0011.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0011.jpg" alt="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" title="Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Analysis of Belclin1. Lane 1: human brain. Lane 2: mouse brain." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 AntibodyBSA Free [NB500-249]</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Analysis of Belclin1. Lane 1: human brain. Lane 2: mouse brain. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB500-249-img0014.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB500-249-img0014.jpg" alt="Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249]" title="Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Analysis of Beclin1 in mouse kidney. Image courtsey of product review submitted by Kelly Hudkins." data-badges="" data-application="Immunohistochemistry-Paraffin" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Analysis of Beclin1 in mouse kidney. Image courtsey of product review submitted by Kelly Hudkins. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-Frozen-NB500-249-img0015.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-Frozen-NB500-249-img0015.jpg" alt="Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249]" title="Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249] - Merged immunostaining of frozen section of Rat brain tissue. Image from verified customer review." data-badges="" data-application="Immunohistochemistry-Frozen" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249] - Merged immunostaining of frozen section of Rat brain tissue. Image from verified customer review. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0016.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0016.jpg" alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on HeLa cells with NB500-249AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700." data-badges="" data-application="Flow Cytometry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on HeLa cells with NB500-249AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0021.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0021.jpg" alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on HepG2 cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher)." data-badges="" data-application="Flow Cytometry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on HepG2 cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher). </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0022.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0022.jpg" alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on THP-1 cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher)." data-badges="" data-application="Flow Cytometry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on THP-1 cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher). </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0023.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0023.jpg" alt="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" title="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on Neuro2a cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher)." data-badges="" data-application="Flow Cytometry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on Neuro2a cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher). </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Simple-Western-NB500-249-img0012.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Simple-Western-NB500-249-img0012.jpg" alt="Simple Western: Beclin 1 AntibodyBSA Free [NB500-249]" title="Simple Western: Beclin 1 AntibodyBSA Free [NB500-249]" data-caption="Simple Western: Beclin 1 Antibody - BSA Free [NB500-249] - Image shows a specific band for Beclin1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Simple Western: Beclin 1 AntibodyBSA Free [NB500-249]</h4> Simple Western: Beclin 1 Antibody - BSA Free [NB500-249] - Image shows a specific band for Beclin1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Knockdown-Validated-NB500-249-img0019.jpg" data-srcset="https://resources.bio-techne.com/images/products/Beclin-1-Antibody---BSA-Free-Knockdown-Validated-NB500-249-img0019.jpg" alt="Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249]" title="Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249]" data-caption="Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249] - siRNA knockdown of becn1 (Beclin 1). Lysates from OKP7 cells treated with non-targeting siRNA (non-target) or an siRNA pool directed against Beclin 1 (becn1) were analyzed by Western blot for Beclin 1 production. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1003394) licensed under a CC-BY license." data-badges="<span class="knockout-validation"></span>" data-application="Knockdown Validated" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <div class="validation-badges-header-wrapper"> <a class="knockout-validation mr-3 d-inline-flex mt-1" href="/reagents/antibodies/antibody-validation" target="_blank" title="Knockout Validation"></a> </div> <h4 class="font-weight-bold">Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249]</h4> Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249] - siRNA knockdown of becn1 (Beclin 1). Lysates from OKP7 cells treated with non-targeting siRNA (non-target) or an siRNA pool directed against Beclin 1 (becn1) were analyzed by Western blot for Beclin 1 production. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1003394) licensed under a CC-BY license. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-210202423454813.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-210202423454813.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a fluorescent reporter plasmid to monitor autophagic flux at 8 h following the indicated treatments. GFP (green) & GFP + mRFP (yellow) fluorescence are observed prior to the fusion of autophagosomes with lysosomes whereas only mRFP (red) fluorescence is present in post-fusion autolysosomes. DIC, differential interference contrast microscopy image. DAPI (blue) staining indicates cell nuclei. (B) Quantification of autolysosomes (red puncta) from (A). F(3,13) = 8.756, p = 0.0019; ∗p < 0.05 when compared to all other groups. (C) Western blotting analysis of the indicated autophagy associated protein levels at 24 h following the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Quantification of dendrite beading from (A). F(3,77) = 6.429, p = 0.0006; ∗p < 0.05 when compared to control cells. Error bars show the SEM. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a fluorescent reporter plasmid to monitor autophagic flux at 8 h following the indicated treatments. GFP (green) & GFP + mRFP (yellow) fluorescence are observed prior to the fusion of autophagosomes with lysosomes whereas only mRFP (red) fluorescence is present in post-fusion autolysosomes. DIC, differential interference contrast microscopy image. DAPI (blue) staining indicates cell nuclei. (B) Quantification of autolysosomes (red puncta) from (A). F(3,13) = 8.756, p = 0.0019; ∗p < 0.05 when compared to all other groups. (C) Western blotting analysis of the indicated autophagy associated protein levels at 24 h following the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Quantification of dendrite beading from (A). F(3,77) = 6.429, p = 0.0006; ∗p < 0.05 when compared to control cells. Error bars show the SEM. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-2102024234681.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-2102024234681.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Metabolic profiling & biochemical assay. (A) Relative abundance of the substrates in the glycolytic pathway & TCA cycle in ORP compared to OCL by targeted metabolic profiling. When compared with OCL heart, ORP hearts have significantly lower glucose-6-phosphate & fructose-6-phosphate (both are glycolytic metabolites), & significantly higher a-ketoglutarate, fumarate, malate, & citrate (all are TCA cycle metabolites). *P < 0.05 compared with OCL. See Table S6 for numerical data. (B) A schematic diagram summarizing the changes in metabolism by rapamycin in old heart. (C) Western blots of autophagic markers show no significant change of LC3 II/I, p62, or beclin-1 in cardiac aging. However, OCR has significantly lower p62 than that in OCL. #P < 0.05 compared with OCL. (D) Both CR & RP significantly reduce the age-dependent increase in protein carbonyls (nmol mL−1). #P < 0.05 compared with OCL. (E). Both CR & RP significantly reduce the age-dependent increase in protein ubiquitination.*P < 0.05 compared with YCL & #P < 0.05 compared with OCL. n = 3–8. G6P: glucose 6-phosphate; G1P: glucose 1-phosphate; F6P: fructose 6-phosphate; F1P: fructose 1-phosphate; F16BP: fructose 1,6-bisphosphate; F26BP: fructose 2,6-biphosphate; G3P: glyceraldehyde 3-phosphate; DHAP:dihydroxyacetone phosphate; 2(3)-PGA: 2- or 3-phosphoglycerate; & PEP: phosphoenolpyruvate. Isomers of same molecular weight, that is, G6P versus G1P, F6P versus F1P, & F16BP versus F26BP, were not distinguishable by the LC-MS/MS-based metabolic profiling method. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24612461), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Metabolic profiling & biochemical assay. (A) Relative abundance of the substrates in the glycolytic pathway & TCA cycle in ORP compared to OCL by targeted metabolic profiling. When compared with OCL heart, ORP hearts have significantly lower glucose-6-phosphate & fructose-6-phosphate (both are glycolytic metabolites), & significantly higher a-ketoglutarate, fumarate, malate, & citrate (all are TCA cycle metabolites). *P < 0.05 compared with OCL. See Table S6 for numerical data. (B) A schematic diagram summarizing the changes in metabolism by rapamycin in old heart. (C) Western blots of autophagic markers show no significant change of LC3 II/I, p62, or beclin-1 in cardiac aging. However, OCR has significantly lower p62 than that in OCL. #P < 0.05 compared with OCL. (D) Both CR & RP significantly reduce the age-dependent increase in protein carbonyls (nmol mL−1). #P < 0.05 compared with OCL. (E). Both CR & RP significantly reduce the age-dependent increase in protein ubiquitination.*P < 0.05 compared with YCL & #P < 0.05 compared with OCL. n = 3–8. G6P: glucose 6-phosphate; G1P: glucose 1-phosphate; F6P: fructose 6-phosphate; F1P: fructose 1-phosphate; F16BP: fructose 1,6-bisphosphate; F26BP: fructose 2,6-biphosphate; G3P: glyceraldehyde 3-phosphate; DHAP:dihydroxyacetone phosphate; 2(3)-PGA: 2- or 3-phosphoglycerate; & PEP: phosphoenolpyruvate. Isomers of same molecular weight, that is, G6P versus G1P, F6P versus F1P, & F16BP versus F26BP, were not distinguishable by the LC-MS/MS-based metabolic profiling method. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24612461), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-210202423454858.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-210202423454858.jpg" alt="Beclin 1 Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Autophagy associated protein immunoreactivity in HIV-infected brain tissue. (A) Representative images from five randomly selected fields of cells each examined in duplicate frontal lobe white matter sections for the indicated subject groups. The indicated proteins were labeled red & microglia with the cell-type-specific marker Iba1 (green). Blue staining indicates cell nuclei. Arrow heads indicate examples of higher Iba1 immunoreactivity whereas arrows indicate more focal (punctal) vs. diffuse (filamentous) patterns of autophagy associated protein expression. Scale bar = 10 μm. (B) Quantification of relative Iba1 immunoreactivity from (A). F(3,20) = 6.450, p = 0.0031; ∗p < 0.05 when compared to all other subject groups. Error bars show the SEM for the average values of 2–6 regions from each subject group across the six autophagy associated proteins examined. (C) Quantification of the indicated autophagy associated protein relative immunoreactivity from (A). Beclin 1: F(3,12) = 11.29, p = 0.0008; LC3B: F(3,12) = 1.994, p = 0.1687; APG7/ATG7: F(3,12) = 84.20, p = < 0.0001; ATG5: F(3,12) = 6.218, p = 0.0086; p62/SQSTM1: F(3,12) = 87.04, p = < 0.0001; LAMP1: F(3,12) = 8.317, p = 0.0029. ∗p < 0.05 when compared to HIV-negative; #p < 0.05 when compared to HIV-positive; & Ωp < 0.05 when compared to HIV-positive/NCI subjects. Error bars show the SEM for four regions from each subject group. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Autophagy associated protein immunoreactivity in HIV-infected brain tissue. (A) Representative images from five randomly selected fields of cells each examined in duplicate frontal lobe white matter sections for the indicated subject groups. The indicated proteins were labeled red & microglia with the cell-type-specific marker Iba1 (green). Blue staining indicates cell nuclei. Arrow heads indicate examples of higher Iba1 immunoreactivity whereas arrows indicate more focal (punctal) vs. diffuse (filamentous) patterns of autophagy associated protein expression. Scale bar = 10 μm. (B) Quantification of relative Iba1 immunoreactivity from (A). F(3,20) = 6.450, p = 0.0031; ∗p < 0.05 when compared to all other subject groups. Error bars show the SEM for the average values of 2–6 regions from each subject group across the six autophagy associated proteins examined. (C) Quantification of the indicated autophagy associated protein relative immunoreactivity from (A). Beclin 1: F(3,12) = 11.29, p = 0.0008; LC3B: F(3,12) = 1.994, p = 0.1687; APG7/ATG7: F(3,12) = 84.20, p = < 0.0001; ATG5: F(3,12) = 6.218, p = 0.0086; p62/SQSTM1: F(3,12) = 87.04, p = < 0.0001; LAMP1: F(3,12) = 8.317, p = 0.0029. ∗p < 0.05 when compared to HIV-negative; #p < 0.05 when compared to HIV-positive; & Ωp < 0.05 when compared to HIV-positive/NCI subjects. Error bars show the SEM for four regions from each subject group. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415304271.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415304271.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Excessive activation of P62-mediated autophagic degradation by the phosphorylated ER alpha-ERK cascades may lead to the autophagic cell death induced by gemcitabine in ER positive MCF-7 cells. B. MCF-7 cells treated by gemcitabine, gemcitabine+ PD98059 (30 μmol/L, added before gemcitabine treatment for 1 h) or DMSO for 48 h. Then total cell lysates subjected to immunoblot analysis with indicated antibodies. The data represented a typical experiment conducted 3times with similar results. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.10363), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Excessive activation of P62-mediated autophagic degradation by the phosphorylated ER alpha-ERK cascades may lead to the autophagic cell death induced by gemcitabine in ER positive MCF-7 cells. B. MCF-7 cells treated by gemcitabine, gemcitabine+ PD98059 (30 μmol/L, added before gemcitabine treatment for 1 h) or DMSO for 48 h. Then total cell lysates subjected to immunoblot analysis with indicated antibodies. The data represented a typical experiment conducted 3times with similar results. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.10363), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241537194.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241537194.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin failed to suppress oxidative stress & activate autophagy in Nrf2−/− mice following TBI.Fucoxanthin treatment had no effect on change the level of MDA & the activity of GPx (A) & the expression of Beclin-1, LC3 & p62 (B) in Nrf2−/− mice compared to the vehicle-treated group. n = 6 per group. @p > 0.05 versus TBI + vehicle group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin failed to suppress oxidative stress & activate autophagy in Nrf2−/− mice following TBI.Fucoxanthin treatment had no effect on change the level of MDA & the activity of GPx (A) & the expression of Beclin-1, LC3 & p62 (B) in Nrf2−/− mice compared to the vehicle-treated group. n = 6 per group. @p > 0.05 versus TBI + vehicle group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415291949.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415291949.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - AMPK knockout interrupts & decreases apoptosis in cochlea. (C) WB results show changes in autophagy-related proteins in cochleae of aging mice. There is a remarkable decline of mTOR signaling (Tg-B1 vs. AMPK+/−/Tg-B1, p<0.0001; Tg-B1 vs. WT, p=0.0001) & more Beclin-1 (Tg-B1 vs. AMPK+/−/Tg-B1, p<0.0001, one-way ANOVA followed by Bonferroni post-test) expressed in cochleae of Tg-B1 mice. (D) The histograms of WB analyses show knockouts of AMPK relieve the ROS-induced autophagic stress in Tg-B1 mice. Analysis performed by using Image J software & one-way ANOVA followed by Bonferroni post-test. * P<0.05, ** P<0.01, ***P<0.001, **** P<0.0001; n=3 per group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32240104), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - AMPK knockout interrupts & decreases apoptosis in cochlea. (C) WB results show changes in autophagy-related proteins in cochleae of aging mice. There is a remarkable decline of mTOR signaling (Tg-B1 vs. AMPK+/−/Tg-B1, p<0.0001; Tg-B1 vs. WT, p=0.0001) & more Beclin-1 (Tg-B1 vs. AMPK+/−/Tg-B1, p<0.0001, one-way ANOVA followed by Bonferroni post-test) expressed in cochleae of Tg-B1 mice. (D) The histograms of WB analyses show knockouts of AMPK relieve the ROS-induced autophagic stress in Tg-B1 mice. Analysis performed by using Image J software & one-way ANOVA followed by Bonferroni post-test. * P<0.05, ** P<0.01, ***P<0.001, **** P<0.0001; n=3 per group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32240104), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415293314.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202415293314.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Baclofen reversed the changes of protein markers characteristic for autophagy in hippocampal CA1 area under chronic cerebral hypoperfusion.(a–c) Five weeks after induction of hypoperfusion, p-mTOR was significantly decreased, & LC3-II, Beclin 1, atg5 & atg7 were significantly increased, & baclofen could reverse the changes of these proteins expression. Treatment with baclofen at 12.5 mg/kg & 25 mg/kg in sham-operated rats did not change the expression of LC3-II, mTOR, p-mTOR, Beclin 1, atg5 & atg7 compared with sham-operated rats (n = 4 in each group). Blots shown have been cropped to fit space requirements & run under the same experimental conditions. *P < 0.05 & **P < 0.01 vs sham-operated rats; ##P < 0.01 vs 2VO rats. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep14474), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Baclofen reversed the changes of protein markers characteristic for autophagy in hippocampal CA1 area under chronic cerebral hypoperfusion.(a–c) Five weeks after induction of hypoperfusion, p-mTOR was significantly decreased, & LC3-II, Beclin 1, atg5 & atg7 were significantly increased, & baclofen could reverse the changes of these proteins expression. Treatment with baclofen at 12.5 mg/kg & 25 mg/kg in sham-operated rats did not change the expression of LC3-II, mTOR, p-mTOR, Beclin 1, atg5 & atg7 compared with sham-operated rats (n = 4 in each group). Blots shown have been cropped to fit space requirements & run under the same experimental conditions. *P < 0.05 & **P < 0.01 vs sham-operated rats; ##P < 0.01 vs 2VO rats. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep14474), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416161847.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416161847.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin protected primary cultured neurons from TBI.(A) Primary cortical neurons were subjected to scratch injury & then treated with 5, 10 or 20 μM fucoxanthin or DMSO for 1 day. The LDH release assay was used to evaluate cell viability. The percentage of survival cells significantly decreased after TBI compared to the control group. Fucoxanthin treatment significantly increased survival cells after TBI. (B) Fucoxanthin repressed the production of ROS in primary cultured cells after TBI. Cells were subjected to scratch injury & subsequently treated with 100 μM edaravone or 5, 10 or 20 μM fucoxanthin or DMSO for 1 day. Then cells were incubated with DCFH-DA & subjected to fluorescence spectrophotometer analysis. The intracellular ROS was significantly increased after TBI compared to the sham group, & administration of edaravone or fucoxanthin significantly repressed ROS production as compared to the TBI + DMSO group. (C) Fucoxanthin inhibited apoptosis & activated autophagy in primary cultured neurons. Primary cortical neurons were subjected to scratch injury & then treated with 5, 10 or 20 μM fucoxanthin or DMSO for 1 day, the expression of cleaved caspase-3, Beclin-1, LC3 & p62 was measured by western blot. Fucoxanthin significantly decreased the expression of cleaved caspase-3 & p62 while increased the expression of Beclin-1 & LC3-II. Data are presented as mean ± SEM, n = 6 per group; *p < 0.05, **p < 0.01, ***p < 0.001 versus control group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus TBI + DMSO group; @p > 0.05 versus TBI + DMSO group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin protected primary cultured neurons from TBI.(A) Primary cortical neurons were subjected to scratch injury & then treated with 5, 10 or 20 μM fucoxanthin or DMSO for 1 day. The LDH release assay was used to evaluate cell viability. The percentage of survival cells significantly decreased after TBI compared to the control group. Fucoxanthin treatment significantly increased survival cells after TBI. (B) Fucoxanthin repressed the production of ROS in primary cultured cells after TBI. Cells were subjected to scratch injury & subsequently treated with 100 μM edaravone or 5, 10 or 20 μM fucoxanthin or DMSO for 1 day. Then cells were incubated with DCFH-DA & subjected to fluorescence spectrophotometer analysis. The intracellular ROS was significantly increased after TBI compared to the sham group, & administration of edaravone or fucoxanthin significantly repressed ROS production as compared to the TBI + DMSO group. (C) Fucoxanthin inhibited apoptosis & activated autophagy in primary cultured neurons. Primary cortical neurons were subjected to scratch injury & then treated with 5, 10 or 20 μM fucoxanthin or DMSO for 1 day, the expression of cleaved caspase-3, Beclin-1, LC3 & p62 was measured by western blot. Fucoxanthin significantly decreased the expression of cleaved caspase-3 & p62 while increased the expression of Beclin-1 & LC3-II. Data are presented as mean ± SEM, n = 6 per group; *p < 0.05, **p < 0.01, ***p < 0.001 versus control group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus TBI + DMSO group; @p > 0.05 versus TBI + DMSO group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416175220.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416175220.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Inhibition of lysosomes leads to G1 arrest by inactivating cyclin E/CDK2 complex. (A) CQ treatment (10, 50, or 100 μM, 24 h) leads to cell cycle G1 arrest. Quantitation of different cell cycle stages in TM3 cells in the presence or absence of CQ. (B–D) EdU incorporation & mitotic index are reduced in CQ-treated TM3 cells. (B) Immunostaining of EdU (red) & DAPI (blue) in scramble control (CTL) or CQ treated TM3 cells. Quantitation of EdU incorporation (C) or mitotic index (D) in scramble control (CTL) or CQ-treated TM3 cells. These results are mean +/− SD from three independent experiments; more than 1000 cells were counted in each individual group. (E–G) CQ inhibited cyclin E1 expression & CDK2 activation. (E,F) Whole cell extracts of CQ-treated TM3 cells at the concentration of 10, 50, or 100 μM are analyzed by immunoblot with antibodies against Beclin1, cyclin D, cyclin A, cyclin E1, CDK2, phosphorylated CDK2 at Thr160 (pCDK2) & alpha-tubulin. (G) Quantitation of relative intensity of cyclin E & pCDK2 in (F). *P < 0.05; **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-00393-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Inhibition of lysosomes leads to G1 arrest by inactivating cyclin E/CDK2 complex. (A) CQ treatment (10, 50, or 100 μM, 24 h) leads to cell cycle G1 arrest. Quantitation of different cell cycle stages in TM3 cells in the presence or absence of CQ. (B–D) EdU incorporation & mitotic index are reduced in CQ-treated TM3 cells. (B) Immunostaining of EdU (red) & DAPI (blue) in scramble control (CTL) or CQ treated TM3 cells. Quantitation of EdU incorporation (C) or mitotic index (D) in scramble control (CTL) or CQ-treated TM3 cells. These results are mean +/− SD from three independent experiments; more than 1000 cells were counted in each individual group. (E–G) CQ inhibited cyclin E1 expression & CDK2 activation. (E,F) Whole cell extracts of CQ-treated TM3 cells at the concentration of 10, 50, or 100 μM are analyzed by immunoblot with antibodies against Beclin1, cyclin D, cyclin A, cyclin E1, CDK2, phosphorylated CDK2 at Thr160 (pCDK2) & alpha-tubulin. (G) Quantitation of relative intensity of cyclin E & pCDK2 in (F). *P < 0.05; **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-00393-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241618261.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241618261.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - 8-Cl-Ado-induces autophagic cell killing. (A) Western blot analysis of beclin1 & ATG7 levels in MCF-7 cells transfected with either a pool of control siRNA (siCONT), siRNA targeting the expression of the beclin1 gene (siBECN1), or targeting the expression of the ATG7 gene (siATG7). Immunoblot analysis of LC3B lipidation & PARP cleavage were assessed as markers of autophagosome formation & apoptosis, respectively. GAPDH was used as loading control. Flow cytometric analysis of cells transfected with siCONT, solid bars, siBECN1, hatched bars, or siATG7, checkered bars, treated with 10 μM 8-Cl-Ado & stained with (B) annexin V & PI, as well as (C) acridine orange. Effect of autophagy on 8-Cl-Ado-inhibiton of clonogenic survival. Cells transfected with (D) siCONT, ○, or siBECN1, ●, & with (E) siCONT, ○, or siATG7, ●, were treated with the indicated doses of 8-Cl-Ado for 3 days, washed with PBS, & cultured in fresh medium for 10 days. Colonies of >50 cells were counted under a dissecting microscope. Image collected & cropped by CiteAb from the following publication (https://jhoonline.biomedcentral.com/articles/10.1186/1756-8722-7-23), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - 8-Cl-Ado-induces autophagic cell killing. (A) Western blot analysis of beclin1 & ATG7 levels in MCF-7 cells transfected with either a pool of control siRNA (siCONT), siRNA targeting the expression of the beclin1 gene (siBECN1), or targeting the expression of the ATG7 gene (siATG7). Immunoblot analysis of LC3B lipidation & PARP cleavage were assessed as markers of autophagosome formation & apoptosis, respectively. GAPDH was used as loading control. Flow cytometric analysis of cells transfected with siCONT, solid bars, siBECN1, hatched bars, or siATG7, checkered bars, treated with 10 μM 8-Cl-Ado & stained with (B) annexin V & PI, as well as (C) acridine orange. Effect of autophagy on 8-Cl-Ado-inhibiton of clonogenic survival. Cells transfected with (D) siCONT, ○, or siBECN1, ●, & with (E) siCONT, ○, or siATG7, ●, were treated with the indicated doses of 8-Cl-Ado for 3 days, washed with PBS, & cultured in fresh medium for 10 days. Colonies of >50 cells were counted under a dissecting microscope. Image collected & cropped by CiteAb from the following publication (https://jhoonline.biomedcentral.com/articles/10.1186/1756-8722-7-23), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416171472.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416171472.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin activated autophagy after TBI.(A) Representative images of immunofluorescence for LC3 surrounding the injured cortex. LC3 punctate dots were observed in the cytoplasm by immunofluorescent staining of LC3 (red). Neuron cells & nuclei are labeled with NeuN (green) & DAPI (blue), respectively. Magnification: 40 x. Scale bar: 50 mm. (B) Mice brain tissues were collected 1 day after TBI in different groups, & the expression of LC3, Beclin-1 & p62 was measured by western blot. Fucoxanthin treatment significantly increased the level of LC3-II & Beclin-1 while decreasing the level of p62 after TBI. (C) 3-MA (400 nM) was injected i.c.v. 30 min before TBI. Mice were then subjected to TBI & treatment of fucoxanthin 30 min after TBI. Pretreatment with 3-MA significantly attenuated fucoxanthin-induced activation of autophagy & suppression of apoptosis & oxidative stress in the ipsilateral cortex. Data are presented as mean ± SEM, n = 6 per group; **p < 0.01, ***p < 0.001 versus sham group; #p < 0.05, ##p < 0.01 versus TBI + vehicle group; &&p < 0.01, &&&p < 0.001 versus TBI + fucoxanthin group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin activated autophagy after TBI.(A) Representative images of immunofluorescence for LC3 surrounding the injured cortex. LC3 punctate dots were observed in the cytoplasm by immunofluorescent staining of LC3 (red). Neuron cells & nuclei are labeled with NeuN (green) & DAPI (blue), respectively. Magnification: 40 x. Scale bar: 50 mm. (B) Mice brain tissues were collected 1 day after TBI in different groups, & the expression of LC3, Beclin-1 & p62 was measured by western blot. Fucoxanthin treatment significantly increased the level of LC3-II & Beclin-1 while decreasing the level of p62 after TBI. (C) 3-MA (400 nM) was injected i.c.v. 30 min before TBI. Mice were then subjected to TBI & treatment of fucoxanthin 30 min after TBI. Pretreatment with 3-MA significantly attenuated fucoxanthin-induced activation of autophagy & suppression of apoptosis & oxidative stress in the ipsilateral cortex. Data are presented as mean ± SEM, n = 6 per group; **p < 0.01, ***p < 0.001 versus sham group; #p < 0.05, ##p < 0.01 versus TBI + vehicle group; &&p < 0.01, &&&p < 0.001 versus TBI + fucoxanthin group. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416165618.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416165618.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - GABARAP interacts with AC3 via LIRs of AC3 in a ciliary expression‐dependent manner. A‐E) WB (A) & densitometric quantification of the expression of GABARAP (B), ATG5 (C), VPS34 (D), & Beclin1(E) in the hypothalami of AC3+/+, AC3−/−, & hAC3 mice (n = 3 mice per group). Actin served as the loading control. F) Representative IF co‐staining with AC3 & GABARAP antibodies in the VMHs of WT mice. F′) A higher magnification of the boxed region. Scale bars: F) 20 µm; F′) 5 µm. G) Representative images showing the expression levels of GABARAP & AC3 in the VMHs of VMH pIFT88‐AC3 KD mice & the controls. Scale bars: 20 µm. H) Schematic representation of GABARAP binding LIRs at aa488‐aa493 & aa958‐aa963 of AC3. I) Co‐IP analysis of GABARAP & AC3 (WT), AC3 (LIR1 Mut), or AC3 (LIR2 Mut). LgG served as the negative control. J) Pull‐down analysis of GABARAP & AC3. K) Co‐IP analysis of GABARAP & AC3 (WT), AC3 (296 Mut), or AC3 (465 Mut). LgG served as the negative control. L) Schematic representation of AC3 regulating GABARAP. Data represent the mean ± SEM; *p < 0.05 & **p < 0.01; one‐way ANOVA & Bonferroni pairwise comparisons. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34783461), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - GABARAP interacts with AC3 via LIRs of AC3 in a ciliary expression‐dependent manner. A‐E) WB (A) & densitometric quantification of the expression of GABARAP (B), ATG5 (C), VPS34 (D), & Beclin1(E) in the hypothalami of AC3+/+, AC3−/−, & hAC3 mice (n = 3 mice per group). Actin served as the loading control. F) Representative IF co‐staining with AC3 & GABARAP antibodies in the VMHs of WT mice. F′) A higher magnification of the boxed region. Scale bars: F) 20 µm; F′) 5 µm. G) Representative images showing the expression levels of GABARAP & AC3 in the VMHs of VMH pIFT88‐AC3 KD mice & the controls. Scale bars: 20 µm. H) Schematic representation of GABARAP binding LIRs at aa488‐aa493 & aa958‐aa963 of AC3. I) Co‐IP analysis of GABARAP & AC3 (WT), AC3 (LIR1 Mut), or AC3 (LIR2 Mut). LgG served as the negative control. J) Pull‐down analysis of GABARAP & AC3. K) Co‐IP analysis of GABARAP & AC3 (WT), AC3 (296 Mut), or AC3 (465 Mut). LgG served as the negative control. L) Schematic representation of AC3 regulating GABARAP. Data represent the mean ± SEM; *p < 0.05 & **p < 0.01; one‐way ANOVA & Bonferroni pairwise comparisons. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34783461), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241616566.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241616566.jpg" alt="Beclin 1 Antibody - BSA Free" title="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -" data-caption="Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - EMPA treatment enhanced autophagy in failing hearts. (A) Transmission electron microscopy (TEM) showed autophagosomes in the hearts. Red arrows point to autophagosomes. (B) EMPA activated autophagy pathway by inhibiting mTOR pathway. (C) RT-PCR results showed that the mRNA expression of Beclin1, Atg7 & LC3 were increased by EMPA treatment after TAC. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; AMPK, AMP-activated protein kinase; mTOR, mammalian target of rapamycin; Ulk, Unc-51 like autophagy activating kinase; Atg7, autophagy related 7; LC3, light chain 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] -</h4> Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - EMPA treatment enhanced autophagy in failing hearts. (A) Transmission electron microscopy (TEM) showed autophagosomes in the hearts. Red arrows point to autophagosomes. (B) EMPA activated autophagy pathway by inhibiting mTOR pathway. (C) RT-PCR results showed that the mRNA expression of Beclin1, Atg7 & LC3 were increased by EMPA treatment after TAC. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; AMPK, AMP-activated protein kinase; mTOR, mammalian target of rapamycin; Ulk, Unc-51 like autophagy activating kinase; Atg7, autophagy related 7; LC3, light chain 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> </span> <div class="row"> <div class="col-md-2"> <a id="scientific-view-more" class="more"> View 24 more images </a> </div> </div> </div> </div> <div id="applications" class="py-5 bt-pp-border-bottom" data-anchor-label="Applications" data-section="section-antibody--applications"> <h3>Applications for Beclin 1 Antibody - BSA Free </h3> <div class="mt-5 py-2 container"> <div class="row"> <div class="col-lg-3 border font-weight-bold text-bg-dark py-2 align-top text-md-left text-white bg-dark-bt-blue d-none d-lg-block"> Application </div> <div class="col-lg-9 border align-top py-2 font-weight-bold text-white bg-dark-bt-blue d-none d-lg-block"> Recommended Usage </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Flow Cytometry</h4> </div> <div class="col-lg-9 border align-top py-2"> 2-10 ug/ml </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunocytochemistry/ Immunofluorescence</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:50-1:200 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunohistochemistry</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:400 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunohistochemistry-Frozen</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:400 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunohistochemistry-Paraffin</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:400 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunoprecipitation</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:80 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Simple Western</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:50 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Western Blot</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:500-1:2000 </div> </div> </div> <div id="application-notes" class="mt-5"> <div class="border-0 font-weight-bold"> Application Notes </div> <div class="product-data-wrapper"> See <a href="https://www.bio-techne.com/resources/simple-western-antibody-database?term=NB500-249">Simple Western Antibody Database</a> for Simple Western validation: Tested in HeLa lysate at 1.0 mg/ml; separated by size; antibody dilution of 1:50. </div> </div> <div class="product-data-wrapper mt-5"> Please Note: Optimal dilutions of this antibody should be experimentally determined. </div> <h4 class="mt-3">Reviewed Applications</h4> <p><span class="review_stars review_stars_4"></span> Read <a href="#reviews" class="reviews">9 reviews</a> rated 3.9 using NB500-249 in the following applications:</p> <ul class="list-unstyled"> <li><a href="#reviews" class="reviews" data-application="Immunohistochemistry">Immunohistochemistry (1 Review) </a></li> <li><a href="#reviews" class="reviews" data-application="Immunohistochemistry-Frozen">Immunohistochemistry-Frozen (1 Review) </a></li> <li><a href="#reviews" class="reviews" data-application="Immunohistochemistry-Paraffin">Immunohistochemistry-Paraffin (2 Reviews) </a></li> <li><a href="#reviews" class="reviews" data-application="Western Blot">Western Blot (5 Reviews) </a></li> </ul> </div> <div id="preparation--storage" class="bt-pp-border-bottom py-5" data-section="section-base--preparation-storage"> <h3>Formulation, Preparation, and Storage</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Purification</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Immunogen affinity purified </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Formulation</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> PBS </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Format</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> BSA Free </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Preservative</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 0.02% Sodium Azide </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Concentration</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 1.0 mg/ml </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Shipping</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Stability & Storage</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. </div> </div> </div> </div> <div id="background" class="py-5 bt-pp-border-bottom" data-section="section-base--background-info"> <h3>Background: Beclin 1</h3> <article class="product-data-wrapper"> Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses. </article> <div id="background_wrapper" class="px-3 mt-2"> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Long Name</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> Beclin1, Autophagy-related </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Alternate Names</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> ATG6, BECN1, GT197, VPS30 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Gene Symbol</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> BECN1 </div> </div> </div> <h4 class="mt-3">Additional Beclin 1 Products</h4> <ul class="list-unstyled"> <li><a href="/t/beclin-1" data-product="Beclin 1">All Products for Beclin 1 </a></li> <li><a href="/t/beclin-1/elisa-kits" data-application="Beclin 1 ELISA Kits">Beclin 1 ELISA Kits </a></li> <li><a href="/t/beclin-1/lysates" data-application="Beclin 1 Lysates">Beclin 1 Lysates </a></li> <li><a href="/t/beclin-1/antibodies" data-application="Beclin 1 Primary Antibodies">Beclin 1 Primary Antibodies </a></li> <li><a href="/t/beclin-1/proteins-enzymes" data-application="Beclin 1 Proteins and Enzymes">Beclin 1 Proteins and Enzymes </a></li> </ul> </div> <div id="product-documents" data-section="section-base--product-documents"> <div id="accordion" class="border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3"> Product Documents for Beclin 1 Antibody - BSA Free </h3> </div> <div class="vtab-section" data-section="Product Datasheets"> <div class="card-header rounded-top"> <a class="" data-toggle="collapse" data-target="#collapse_two" 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Beclin 1 Antibody - BSA Free (NB500-249) by Novus, Part of Bio-Techne