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<!doctype html> <html lang="es"> <head> <title>Understanding Sample Preparation Workflow: Semi-Automated Pr | 1099778</title> <meta name="keywords" content="Ramisa Fariha, Collin Hill, Mohannad Jabrah, Adam Spooner, Anubhav Tripathi* , "/> <meta name="description" content="High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the pro..1099778"/> <meta name="citation_publisher" content="Longdom Publicaci贸n SL" /> <meta name="citation_journal_title" content="Androlog铆a-Acceso Abierto"> <meta name="citation_title" content="Understanding Sample Preparation Workflow: Semi-Automated Preparation of Serum Testosterone for Detection Using LC-MS/MS "> <meta name="citation_author" content="Ramisa Fariha" /> <meta name="citation_author" content="Collin Hill" /> <meta name="citation_author" content="Mohannad Jabrah" /> <meta name="citation_author" content="Adam Spooner" /> <meta name="citation_author" content="Anubhav Tripathi" /> <meta name="citation_year" content=""> <meta name="citation_volume" content="13"> <meta name="citation_issue" content="5"> <meta name="citation_abstract" content="High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the progression of different metabolic disorders. Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS® G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), within a clinically relevant range of testosterone serum concentrations (5-1500 ng/dl). Moreover, the assay showcased LOD of 5 ng/dl, which is significantly comparable to commercially established diagnostic tools. The developed method also exhibited high linearity over the specified testosterone serum range, with an obtained linearity of R2>0.99 using both manual as well as automated sample preparation. Overall, the work shown presents an optimized, automatable LC-MS/MSbased testosterone diagnostic assay for the routine evaluation of patient testosterone levels in serum for clinical and research use. 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class="nav-item nav-link" href="https://spanish.longdom.org/chemistry-journals.html" title="haga clic aqu铆">Qu铆mica</a><br> </nav> </div> </div>  </aside> <div class="col-12 col-sm-9"> <h2 class="heading-1">abstracto</h2> <h3 class="">Understanding Sample Preparation Workflow: Semi-Automated Preparation of Serum Testosterone for Detection Using LC-MS/MS </h3> <p> <i class="fas fa-user"></i> Ramisa Fariha, Collin Hill, Mohannad Jabrah, Adam Spooner, Anubhav Tripathi* </p> <p>High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the progression of different metabolic disorders. Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS<sup>® </sup>G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), within a clinically relevant range of testosterone serum concentrations (5-1500 ng/dl). Moreover, the assay showcased LOD of 5 ng/dl, which is significantly comparable to commercially established diagnostic tools. The developed method also exhibited high linearity over the specified testosterone serum range, with an obtained linearity of R2>0.99 using both manual as well as automated sample preparation. Overall, the work shown presents an optimized, automatable LC-MS/MSbased testosterone diagnostic assay for the routine evaluation of patient testosterone levels in serum for clinical and research use.</p> <div class="alert alert-info text-left"><b>Descargo de responsabilidad:</b> este resumen se tradujo utilizando herramientas de inteligencia artificial y a煤n no ha sido revisado ni verificado.</div> <div class="nav social-icons"> <a class="nav-link w-auto">Comparte este art铆culo</a> <a title="haga clic aqu铆" target="_blank" href="https://www.facebook.com/sharer.php?u=https://spanish.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-facebook-f"></i></a> <a class="nav-link" title="haga clic aqu铆" target="_blank" href="https://twitter.com/share?url=https://spanish.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-twitter"></i></a> <a class="nav-link" title="haga clic aqu铆" target="_blank" href="https://www.linkedin.com/shareArticle?mini=true&url=https://spanish.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link" title="haga clic aqu铆" target="_blank" href="https://plus.google.com/share?url=https://spanish.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-google-plus-g"></i></a> </div> </div> </div> </div> </section> <footer class="bg-blue-grey-900 py-3"> <div class="container"> <div class="row"> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Enlaces de contenido</h4> <ul class="list-unstyled footer-links font-size-3"> <li><a class="" href="https://spanish.longdom.org/privacy-policy.html" title="haga clic aqu铆">Pol铆tica de Privacidad</a></li> <li><a class="" href="https://spanish.longdom.org/terms-conditions.html" title="haga clic aqu铆">T茅rminos y condiciones Condiciones</a></li> <li><a class="" href="https://spanish.longdom.org/authors-reviewers-editors.html" title="haga clic aqu铆">Autores, revisores y editor</a></li> </ul> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Contacto Longdom</h4> <p><font style="vertical-align:inherit"><font style="vertical-align:inherit">Longdom Group SA </font></font><br /> <font style="vertical-align:inherit"><font style="vertical-align:inherit">Avenue Roger Vandendriessche, </font></font><br /> <font style="vertical-align:inherit"><font style="vertical-align:inherit">18, 1150 Bruselas, Bélgica </font></font><br /> <font style="vertical-align:inherit"><font style="vertical-align:inherit">Teléfono: +442038085340 </font></font><br /> <strong><font style="vertical-align:inherit"><font style="vertical-align:inherit">Correo electrónico: </font></font></strong> <a class="white" href="mailto:info@longdom.org" title="haga clic aqu铆"><font style="vertical-align:inherit"><font style="vertical-align:inherit">info@longdom.org</font></font></a></p> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Conectar</h4> <nav class="nav nav-pills social-icons-footer flex-column a-pl-0"> <a href="https://www.facebook.com/longdompublisher" title="haga clic aqu铆" target="_blank" class="nav-link bg-facebook-hover"><i class="fab fa-facebook-f bg-facebook"></i></a> <a href="https://www.linkedin.com/company/longdom-publishing-sl/" title="haga clic aqu铆" target="_blank" class="nav-link bg-linkedin-hover"><i class="fab fa-linkedin-in bg-linkedin"></i></a> <a href="https://twitter.com/LongdomP" title="haga clic aqu铆" target="_blank" class="nav-link bg-twitter-hover"><i class="fab fa-twitter bg-twitter"></i></a> <a href="https://www.instagram.com/longdom_publisher/" title="haga clic aqu铆" target="_blank" class="nav-link bg-instagram-hover"><i class="fab fa-instagram bg-instagram"></i></a> </nav> </div> </div> <div class="row text-center"> <div class="col"> <p>derechos de autor © 2024 <a href="https://spanish.longdom.org/" title="haga clic aqu铆" class="white">Longdom Publicaci贸n SL</a>.</p> </div> </div> </div> </footer>  <a href="#0" class="cd-top js-cd-top">Top</a>   <script src="https://code.jquery.com/jquery-3.3.1.min.js"></script> <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js"></script> <script src="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js"></script>  <script> jQuery(function($) { $(window).scroll(function fix_element() { $('#target').css( $(window).scrollTop() > 100 ? 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