Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay (LHSCM210): R&D Systems

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r.ee.get(this.agentIdentifier).abort(),!1}}}({features:[R,_,_e,Ne,he,N,L,Pe,ke],loaderType:"pro"})})()})();</script> <link rel="profile" href="http://www.w3.org/1999/xhtml/vocab" /> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="keywords" content="" /> <link rel="canonical" href="https://www.rndsystems.com/products/human-tnf-alpha-high-sensitivity-magnetic-luminex-performance-assay_lhscm210" /> <meta refresh_date="11/22/2024 11:53:56 PM" /> <title>Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay (LHSCM210): R&D Systems</title> <meta name="description" content="Build your own Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay with our Luminex Assay Customization Tool. Our Luminex multiplexing immunoassays include cytokines and chemokines and are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Cited in 10 publications." /> <meta http-equiv="content-language" content="en-us" /> <link href="https://www.rndsystems.com/products/human-tnf-alpha-high-sensitivity-magnetic-luminex-performance-assay_lhscm210" rel="alternate" hreflang="x-default" /> <link href="https://www.rndsystems.com/products/human-tnf-alpha-high-sensitivity-magnetic-luminex-performance-assay_lhscm210" rel="alternate" hreflang="en" /> <link href="https://www.rndsystems.com/cn/products/human-tnf-alpha-high-sensitivity-magnetic-luminex-performance-assay_lhscm210" rel="alternate" hreflang="zh-cn" /> <meta name="baidu-site-verification" content="NoHNFT2tiZ" /> <script id="json-ld" type="application/ld+json">[{"@context":"http://schema.org","@type":"Product","aggregateRating":[],"offers":{"@type":"Offer","price":189,"priceCurrency":"USD"},"url":"https://www.rndsystems.com/products/human-tnf-alpha-high-sensitivity-magnetic-luminex-performance-assay_lhscm210","name":"Human TNF-alpha High Sensitivity Magnetic Luminex\u00ae Performance Assay","productID":"LHSCM210","mpn":"LHSCM210","sku":"LHSCM210","brand":{"@type":"Brand","name":"R&D Systems"},"itemcondition":"new","image":["https://resources.rndsystems.com/images/datasheets/lhscm210_human-tnf-alpha-hs-magnetic-luminex-performance-assay-30620219309.png"],"review":[],"description":"<div class=\"\">\r\n<div class=\"\">\r\n<h3>Precision</h3>\r\n</div>\r\n\r\n<table class=\"table table-bordered table-striped\">\r\n\t<thead>\r\n\t\t<tr>\r\n\t\t\t<td align=\"left\" colspan=\"7\">\r\n\t\t\t<p><strong>Intra-assay Precision</strong> (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.<br />\r\n\t\t\t<strong>Inter-assay Precision</strong> (precision between assays) - Three samples of known concentration were tested in sixty separate assays to assess precision between assays.</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<th> </th>\r\n\t\t\t<th colspan=\"3\" rowspan=\"1\">Intra-Assay Precision</th>\r\n\t\t\t<th colspan=\"3\" rowspan=\"1\">Inter-Assay Precision</th>\r\n\t\t</tr>\r\n\t</thead>\r\n\t<tbody>\r\n\t\t<tr>\r\n\t\t\t<td>Sample</td>\r\n\t\t\t<td style=\"text-align: center;\">1</td>\r\n\t\t\t<td style=\"text-align: center;\">2</td>\r\n\t\t\t<td style=\"text-align: center;\">3</td>\r\n\t\t\t<td style=\"text-align: center;\">1</td>\r\n\t\t\t<td style=\"text-align: center;\">2</td>\r\n\t\t\t<td style=\"text-align: center;\">3</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>n</td>\r\n\t\t\t<td style=\"text-align: center;\">20</td>\r\n\t\t\t<td style=\"text-align: center;\">20</td>\r\n\t\t\t<td style=\"text-align: center;\">20</td>\r\n\t\t\t<td style=\"text-align: center;\">60</td>\r\n\t\t\t<td style=\"text-align: center;\">60</td>\r\n\t\t\t<td style=\"text-align: center;\">60</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>Mean (pg/mL)</td>\r\n\t\t\t<td style=\"text-align: center;\">8.1</td>\r\n\t\t\t<td style=\"text-align: center;\">65</td>\r\n\t\t\t<td style=\"text-align: center;\">1533</td>\r\n\t\t\t<td style=\"text-align: center;\">7.5</td>\r\n\t\t\t<td style=\"text-align: center;\">61</td>\r\n\t\t\t<td style=\"text-align: center;\">1654</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>Standard deviation</td>\r\n\t\t\t<td style=\"text-align: center;\">0.4</td>\r\n\t\t\t<td style=\"text-align: center;\">2.7</td>\r\n\t\t\t<td style=\"text-align: center;\">105</td>\r\n\t\t\t<td style=\"text-align: center;\">0.8</td>\r\n\t\t\t<td style=\"text-align: center;\">4.9</td>\r\n\t\t\t<td style=\"text-align: center;\">169</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CV (%)</td>\r\n\t\t\t<td style=\"text-align: center;\">4.9</td>\r\n\t\t\t<td style=\"text-align: center;\">4.1</td>\r\n\t\t\t<td style=\"text-align: center;\">6.8</td>\r\n\t\t\t<td style=\"text-align: center;\">10.7</td>\r\n\t\t\t<td style=\"text-align: center;\">8.0</td>\r\n\t\t\t<td style=\"text-align: center;\">10.2</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n \r\n\r\n<div class=\"\">\r\n<h3>Recovery</h3>\r\n</div>\r\n\r\n<table class=\"table table-bordered table-striped\">\r\n\t<thead>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"7\">\r\n\t\t\t<p>Samples containing and/or spiked with human TNF-alpha were evaluated for recovery.</p>\r\n </td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<th colspan=\"5\" style=\"text-align: left;\">Sample Type</th>\r\n\t\t\t<th>Average % Recovery</th>\r\n\t\t\t<th>Range</th>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"5\">Cell culture supernates</td>\r\n\t\t\t<td style=\"text-align: center;\">105</td>\r\n\t\t\t<td style=\"text-align: center;\">99-111%</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"5\">Serum</td>\r\n\t\t\t<td style=\"text-align: center;\">86</td>\r\n\t\t\t<td style=\"text-align: center;\">73-101%</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"5\">EDTA plasma</td>\r\n\t\t\t<td style=\"text-align: center;\">79</td>\r\n\t\t\t<td style=\"text-align: center;\">73-87%</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"5\">Heparin plasma</td>\r\n\t\t\t<td style=\"text-align: center;\">79</td>\r\n\t\t\t<td style=\"text-align: center;\">70-94%</td>\r\n\t\t</tr>\r\n\t</thead>\r\n\t<tfoot>\r\n\t</tfoot>\r\n\t<tbody>\r\n\t</tbody>\r\n</table>\r\n \r\n\r\n<div class=\"\">\r\n<h3>Linearity</h3>\r\n</div>\r\n\r\n<table class=\"table table-bordered table-striped\">\r\n\t<thead>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"10\">\r\n\t\t\t<p>Samples containing and/or spiked with human TNF-alpha were serially diluted to evaluate assay linearity.</p>\r\n </td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<th colspan=\"6\" rowspan=\"1\" style=\"text-align: left;\"> </th>\r\n\t\t\t<th>Cell culture supernates*</th>\r\n\t\t\t<th>Serum</th>\r\n\t\t\t<th>EDTA plasma</th>\r\n\t\t\t<th>Heparin plasma</th>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"5\" rowspan=\"2\">1:2</td>\r\n\t\t\t<td>Average % of Expected</td>\r\n\t\t\t<td>98</td>\r\n\t\t\t<td>108</td>\r\n\t\t\t<td>111</td>\r\n\t\t\t<td>112</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>Range (%)</td>\r\n\t\t\t<td>95-101</td>\r\n\t\t\t<td>98-116</td>\r\n\t\t\t<td>107-115</td>\r\n\t\t\t<td>110-113</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"5\" rowspan=\"2\">1:4</td>\r\n\t\t\t<td>Average % of Expected</td>\r\n\t\t\t<td>94</td>\r\n\t\t\t<td>112</td>\r\n\t\t\t<td>115</td>\r\n\t\t\t<td>111</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>Range (%)</td>\r\n\t\t\t<td>93-96</td>\r\n\t\t\t<td>105-125</td>\r\n\t\t\t<td>97-128</td>\r\n\t\t\t<td>107-117</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"5\" rowspan=\"2\">1:8</td>\r\n\t\t\t<td>Average % of Expected</td>\r\n\t\t\t<td>93</td>\r\n\t\t\t<td>116</td>\r\n\t\t\t<td>114</td>\r\n\t\t\t<td>116</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>Range 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id="content_column"> <div class="ds_title_table"> <h1 class="ds_title">Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: LHSCM210</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 10 Citations </a> </span> <a href="/reviews/LHSCM210" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="datasheet_luminex_availability_container col-xs-12 col-md-8 col-md-push-4"> <p class="datasheet_luminex_availability_text"> Build your own Luminex Assay with our Luminex Assay Customization Tool. </p> <p class="datasheet_luminex_availability"> U.S. orders usually ship in 2-3 business days </p> <div style="margin-bottom: 10px;"></div> <a href="#luminex_analyte_page" class="btn btn-primary">Build Now</a> </div> <div class="product_image_wrapper col-xs-12 col-md-4 col-md-pull-8"> <div class="bestseller_product"><span>Best Seller</span></div> <div class="product_image"> <span class="product_image_label"> </span> <a class="ds-image overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="Quantist software graph of signal intensity versus concentration for TNF-alpha in the Human High Sensitivity Cytokine A Luminex Panel" data-footer="Quantist software graph of signal intensity versus concentration for TNF-alpha in the Human High Sensitivity Cytokine A Luminex Panel" title="Cat. No. LHSCM210 - Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay" href="https://resources.rndsystems.com/images/datasheets/lhscm210_human-tnf-alpha-hs-magnetic-luminex-performance-assay-30620219309.png"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/lhscm210_human-tnf-alpha-hs-magnetic-luminex-performance-assay-30620219309.png" class="img-fluid" alt="Quantist software graph of signal intensity versus concentration for TNF-alpha in the Human High Sensitivity Cytokine A Luminex Panel" title="Quantist software graph of signal intensity versus concentration for TNF-alpha in the Human High Sensitivity Cytokine A Luminex Panel"> </a> <div class="search_image_other_count"> <span class="search_more_images">1 Image</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/tnf-alpha?category=Luminex Assays" class="btn btn-secondary">All TNF-alpha Luminex Assays</a> </div> </div> <div class="tabbed_content" id="rnd_luminex"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (10)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#luminex_analyte_page" id="product-anchor-luminex_analyte_page">Configure Your Assay</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#luminex" id="product-anchor-luminex">Product Features</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#luminex_accuracy_and_precision" id="product-anchor-luminex_accuracy_and_precision">Precision & Accuracy</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> </div> <div id="ds_summary" class="datasheet_section"> <h2 class="datasheet-product-title">Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay Summary</h2> <table class="technical_data_table"> <tbody> <tr> <td class="tdt_name">Panel</td> <td class="tdt_data">Luminex Performance Human High Sensitivity Cytokine Panel A<br /> Luminex Performance Human High Sensitivity Cytokine Panel B</td> </tr> <tr> <td class="tdt_name">Assay Type</td> <td class="tdt_data">Magnetic bead-based multiplex assay for the Luminex<sup>®</sup> platform</td> </tr> <tr> <td class="tdt_name">Format</td> <td class="tdt_data">1 x 96-well microplate and Magnetic antibody-coated beads</td> </tr> <tr> <td class="tdt_name">Species</td> <td class="tdt_data">Human</td> </tr> <tr> <td class="tdt_name">Analytes Detected</td> <td class="tdt_data">Please see analyte list in assay customization tool below.</td> </tr> <tr> <td class="tdt_name">Performance Validation</td> <td class="tdt_data">Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.<br/><br/></td> </tr> </tbody> </table> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="LHSCM210"></div> <div id="luminex_analyte_page" class="page-container luminex-analyte-page"> <div class="row"> <div class="luminex-whole-column col-xs-12 col-sm-12"> <h2 class="luminex-analytes-summary">Make your selections below to configure your assay</h2> <div class="luminex-panels-area"> <div class="luminex-panels-area-message builder-header"> <h4>Please choose one of the panels to display the corresponding analytes.</h4> </div>  <select class="luminex-basic-panel-select form-control"> <option value="">-- Choose a Panel --</option> <option value="Luminex Performance Human High Sensitivity Cytokine Panel A">Luminex Performance Human High Sensitivity Cytokine Panel A</option> <option value="Luminex Performance Human High Sensitivity Cytokine Panel B">Luminex Performance Human High Sensitivity Cytokine Panel B</option> </select>  </div>  <div class="luminex-analytes-filter-container luminex-hide-on-load"> <div class="luminex-analytes-filter-element-wrapper"> <span class="luminex-analytes-filter-element luminex-analytes-filter-label">Filter Analytes</span> <span class="luminex-analytes-filter-element luminex-analytes-filter-input-area"> <input type="text" class="luminex-analytes-filter-input" id="luminex_input_filter_results" placeholder="Analyte"/> <span class="luminex-button luminex-button-filter-clear luminex-hide-on-load" id="luminex_button_filter_clear"></span> </span>  </div>  <div class="luminex-analytes-select-wrapper"> <span class="luminex-analytes-filter-element luminex-analytes-select-all-container luminex-button-wrapper"> <span class="btn btn-quinary luminex-button luminex-button-select-all" id="luminex_button_select_all">Select All</span> </span>  <span class="luminex-analytes-filter-element luminex-analytes-select-none-container luminex-button-wrapper"> <span class="btn btn-quinary luminex-button luminex-button-select-none" id="luminex_button_select_none">Select None</span> </span>  </div>  </div>  <div class="luminex-analytes-results row" id="luminex_analytes_results"> </div>  <div class="luminex-assays-section luminex-right-side luminex-hide-on-load"> <div class="row"> <div class="luminex-kits-header col-xs-12"> <h2 class="luminex-kits-summary">Assays</h2> <p class="luminex-kits-instructions"> Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed. </p> <p><b>If you run into bead region incompatibilities, <a href="https://www.rndsystems.com/services/luminex-assays-custom-development-services" target="_blank">send your Luminex code</a> to our team and find out more.</b></p> </div> </div> <div class="luminex-splitting-area row"> <div class="luminex-splitting-mixing luminex-splitting-box col-xs-12 col-sm-6" id="luminex_splitting_mixing"> <div class="luminex-splitting-row luminex-splitting-row-header"> <h4 class="luminex-mixing-question luminex-splitting-question">How would you like these analytes mixed?</h4> </div>  <div class="luminex-splitting-row luminex-splitting-row-premixed"> <div class="luminex-splitting-row-select-premixed luminex-splitting-row-select-mixing luminex-splitting-row-select luminex-mixing-selected" data-mixing="premixed">Premixed<span class="mixing-info-icon structure-info-icon info-icon luminex-info-icon-mixing luminex-info-icon-premixed" data-placement="auto left" data-title="Beads and biotin antibodies are mixed before shipment" data-group="luminex-info-icon-mixing" data-name="luminex-info-icon-premixed"></span></div> </div>  <div class="luminex-splitting-row luminex-splitting-row-user-mixed"> <div class="luminex-splitting-row-select-user-mixed luminex-splitting-row-select-mixing luminex-splitting-row-select" data-mixing="user-mixed">User Mixed<span class="mixing-info-icon structure-info-icon info-icon luminex-info-icon-mixing luminex-info-icon-user-mixed" data-placement="auto left" data-title="Mix your own beads and biotin antibodies when your need them" data-group="luminex-info-icon-mixing" data-name="luminex-info-icon-user-mixed"></span></div> </div>  <div class="luminex-splitting-row-alert luminex-hide-on-load"> <div class="luminex-splitting-row-alert-content alert alert-warning">Please select at least one mixing option to see the kits.</div> </div>  </div>  <div class="luminex-splitting-dilution luminex-splitting-box col-xs-12 col-sm-6" id="luminex_splitting_dilution"> <div class="luminex-splitting-row luminex-splitting-row-header"> <h4 class="luminex-dilution-question luminex-splitting-question">Would you like to split based on dilution?</h4> </div>  <div class="luminex-splitting-row luminex-splitting-row-do-not-split"> <div class="luminex-splitting-row-select-do-not-split luminex-splitting-row-select-dilution luminex-splitting-row-select luminex-dilution-selected" data-dilution="do-not-split">Do not split<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-" data-placement="auto left" data-title="Different sample type dilutions are not considered" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-do-not-split"></span></div> </div>  <div class="luminex-splitting-row luminex-splitting-row-split-serum"> <div class="luminex-splitting-row-select-split-serum luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-serum">Split based on serum<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-serum" data-placement="auto left" data-title="Panel is split based upon matching serum sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-serum"></span></div> </div>  <div class="luminex-splitting-row luminex-splitting-row-split-plasma"> <div class="luminex-splitting-row-select-split-plasma luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-plasma">Split based on plasma<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-plasma" data-placement="auto left" data-title="Panel is split based upon matching plasma sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-plasma"></span></div> </div>  <div class="luminex-splitting-row luminex-splitting-row-split-cell-culture"> <div class="luminex-splitting-row-select-split-cell-culture luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-cell-culture">Split based on cell culture supernates<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-cell-culture" data-placement="auto left" data-title="Panel is split based upon matching cell culture supernate sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-cell-culture"></span></div> </div>  </div>  </div>  <div class="luminex-kits-area row" id="luminex_kits_area"> </div>  </div>  </div>  </div>  </div>  <div id="luminex" class="datasheet_section"><div class="datasheet-luminex-content"> <p class="luminex-text"> Not finding your analyte of interest? View our complete Luminex offering in the full version of our <a href="/luminex/analytes">Luminex Assay Customization Tool</a>. We also offer <a href="/services/luminex-assays-custom-development-services">Custom Luminex Services</a>. </p> <div class="luminex-text-group luminex-product-features"> <div class="ds_lightBlueBar">Product Features</div> <p class="luminex-text"> Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples. </p> <ul class="luminex-list luminex-product-features-list"> <li class="luminex-list-item"> Simultaneously profile Human TNF-alpha with up to <span class="luminex-analytes-displayed-minus-one"></span> analytes of your choice in one sample </li> <li class="luminex-list-item">Magnetic format allows for easier wash steps</li> <li class="luminex-list-item">Undergoes similar validation testing as Quantikine<sup>®</sup> ELISA Kits</li> <li class="luminex-list-item">Precise and Specific</li> <li class="luminex-list-item">Requires a small sample volume (<50 µL)</li> <li class="luminex-list-item">Run your assay in just 3-5 hours</li> <li class="luminex-list-item">Mass-calibrated standards for consistent results with every new lot of material</li> <li class="luminex-list-item">Enough reagents for one 96-well plate</li> </ul>  </div>  <div class="luminex-text-group luminex-kit-components"> <div class="ds_lightBlueBar"> Base Kit Components </div> <ul class="luminex-list luminex-kit-components-list"> <li class="luminex-list-item">Standard Cocktail(s)</li> <li class="luminex-list-item">Bead Diluent</li> <li class="luminex-list-item">Biotin Antibody Diluent</li> <li class="luminex-list-item">Calibrator Diluent</li> <li class="luminex-list-item">Wash Buffer</li> <li class="luminex-list-item">Streptavidin-PE</li> <li class="luminex-list-item">One flat-bottom 96-well Microplate</li> <li class="luminex-list-item">Foil Plate Sealers (4)</li> <li class="luminex-list-item">Mixing Bottle</li> <li class="luminex-list-item">Standard Value Card**</li> </ul>  <p class="luminex-text luminex-note"> **A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. </p> </div>  <div class="luminex-text-group luminex-bead-set-contents"> <div class="ds_lightBlueBar"> Bead Set Contents </div> <ul class="luminex-list luminex-bead-set-list"> <li class="luminex-list-item">User-mix antibody-coated Magnetic beads in individual vials</li> <li class="luminex-list-item">User-mixed cocktail of biotinylated detection antibody</li> </ul>  </div>  <div id="luminex_accuracy_and_precision"> <div class=""> <div class=""> <h3>Precision</h3> </div> <table class="table table-bordered table-striped"> <thead> <tr> <td align="left" colspan="7"> <p><strong>Intra-assay Precision</strong> (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.<br /> <strong>Inter-assay Precision</strong> (precision between assays) - Three samples of known concentration were tested in sixty separate assays to assess precision between assays.</p> </td> </tr> <tr> <th> </th> <th colspan="3" rowspan="1">Intra-Assay Precision</th> <th colspan="3" rowspan="1">Inter-Assay Precision</th> </tr> </thead> <tbody> <tr> <td>Sample</td> <td style="text-align: center;">1</td> <td style="text-align: center;">2</td> <td style="text-align: center;">3</td> <td style="text-align: center;">1</td> <td style="text-align: center;">2</td> <td style="text-align: center;">3</td> </tr> <tr> <td>n</td> <td style="text-align: center;">20</td> <td style="text-align: center;">20</td> <td style="text-align: center;">20</td> <td style="text-align: center;">60</td> <td style="text-align: center;">60</td> <td style="text-align: center;">60</td> </tr> <tr> <td>Mean (pg/mL)</td> <td style="text-align: center;">8.1</td> <td style="text-align: center;">65</td> <td style="text-align: center;">1533</td> <td style="text-align: center;">7.5</td> <td style="text-align: center;">61</td> <td style="text-align: center;">1654</td> </tr> <tr> <td>Standard deviation</td> <td style="text-align: center;">0.4</td> <td style="text-align: center;">2.7</td> <td style="text-align: center;">105</td> <td style="text-align: center;">0.8</td> <td style="text-align: center;">4.9</td> <td style="text-align: center;">169</td> </tr> <tr> <td>CV (%)</td> <td style="text-align: center;">4.9</td> <td style="text-align: center;">4.1</td> <td style="text-align: center;">6.8</td> <td style="text-align: center;">10.7</td> <td style="text-align: center;">8.0</td> <td style="text-align: center;">10.2</td> </tr> </tbody> </table>   <div class=""> <h3>Recovery</h3> </div> <table class="table table-bordered table-striped"> <thead> <tr> <td colspan="7"> <p>Samples containing and/or spiked with human TNF-alpha were evaluated for recovery.</p> </td> </tr> <tr> <th colspan="5" style="text-align: left;">Sample Type</th> <th>Average % Recovery</th> <th>Range</th> </tr> <tr> <td colspan="5">Cell culture supernates</td> <td style="text-align: center;">105</td> <td style="text-align: center;">99-111%</td> </tr> <tr> <td colspan="5">Serum</td> <td style="text-align: center;">86</td> <td style="text-align: center;">73-101%</td> </tr> <tr> <td colspan="5">EDTA plasma</td> <td style="text-align: center;">79</td> <td style="text-align: center;">73-87%</td> </tr> <tr> <td colspan="5">Heparin plasma</td> <td style="text-align: center;">79</td> <td style="text-align: center;">70-94%</td> </tr> </thead> <tfoot> </tfoot> <tbody> </tbody> </table>   <div class=""> <h3>Linearity</h3> </div> <table class="table table-bordered table-striped"> <thead> <tr> <td colspan="10"> <p>Samples containing and/or spiked with human TNF-alpha were serially diluted to evaluate assay linearity.</p> </td> </tr> <tr> <th colspan="6" rowspan="1" style="text-align: left;"> </th> <th>Cell culture supernates*</th> <th>Serum</th> <th>EDTA plasma</th> <th>Heparin plasma</th> </tr> <tr> <td colspan="5" rowspan="2">1:2</td> <td>Average % of Expected</td> <td>98</td> <td>108</td> <td>111</td> <td>112</td> </tr> <tr> <td>Range (%)</td> <td>95-101</td> <td>98-116</td> <td>107-115</td> <td>110-113</td> </tr> <tr> <td colspan="5" rowspan="2">1:4</td> <td>Average % of Expected</td> <td>94</td> <td>112</td> <td>115</td> <td>111</td> </tr> <tr> <td>Range (%)</td> <td>93-96</td> <td>105-125</td> <td>97-128</td> <td>107-117</td> </tr> <tr> <td colspan="5" rowspan="2">1:8</td> <td>Average % of Expected</td> <td>93</td> <td>116</td> <td>114</td> <td>116</td> </tr> <tr> <td>Range (%)</td> <td>90-97</td> <td>105-139</td> <td>99-129</td> <td>110-123</td> </tr> </thead> </table> <h3><br /> *Cell culture supernates are valid samples in Human HS Cytokine Base Kit B only.</h3> </div> </div> <div class="luminex-text-group luminex-right-for-me"> <div class="ds_lightBlueBar"> Which Luminex Assay is right for me? </div> <p class="luminex-text"> Assays for the Luminex platform are offered as <a href="/products/luminex-high-performance-assays">High Performance Assays</a> or <a href="/products/luminex-assays">Assays</a>. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. <a href="/products/which-luminex-assay-right-me">View a table comparing the features and benefits of these bead-based multiplex assays.</a> </p> </div>  <div class="luminex-text-group luminex-more-information"> <div class="ds_lightBlueBar"> More Information About Magnetic Bead-based Multiplex Kits For The Luminex<sup>®</sup> Platform </div> <p class="luminex-text"> Magnetic <a href="/products/luminex-high-performance-assays">Luminex High Performance Assays</a> are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the <a href="/products/luminex-200-ruo-system-w-xponent-42_lx200-xpon-ruo">Luminex 100/200™</a> or <a href="/products/flexmap-3d-with-xponent-42_flexmap-3d-ruo">FLEXMAP 3D</a>, dual laser, flow-based sorting and detection platforms. </p> <p class="luminex-text"> Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems. </p> </div>  </div>  </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> N/A </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="Quantist software graph of signal intensity versus concentration for TNF-alpha in the Human High Sensitivity Cytokine A Luminex Panel" data-footer="" title="Cat. No. LHSCM210 - " href="https://resources.rndsystems.com/images/datasheets/lhscm210_human-tnf-alpha-hs-magnetic-luminex-performance-assay-30620219309.png"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/lhscm210_human-tnf-alpha-hs-magnetic-luminex-performance-assay-30620219309.png" class="img-fluid" alt="" title=""> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Quantist software graph of signal intensity versus concentration for TNF-alpha in the Human High Sensitivity Cytokine A Luminex Panel</span> </p> </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: TNF-alpha</h2> <p><p>Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.</p> <p>Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.</p></p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name</div> <div class="ds_table_cell background_table_data">Tumor Necrosis Factor alpha</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs</div> <div class="ds_table_cell background_table_data">7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names</div> <div class="ds_table_cell background_table_data">APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha</div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-tnf-alpha-high-sensitivity-magnetic-luminex-performance-assay_lhscm210">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//resources.rndsystems.com/pdfs/msds/lhscm210.pdf?v=20241123">SDS</a> </div> </div> </div> <div class="ds_lightBlueBar">Product Specific Notices</div> <div class="animal_bluebar"> Luminex® is a registered trademark of Luminex Corporation. </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">10</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">10</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Human"> Human </option> <option class="citation_filterRadio" value="species" data-filter="Rat"> Rat </option> </select> </div> <input type="hidden" id="selAntibody" value="All"> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="Cell Culture Supernates"> Cell Culture Supernates </option> <option class="citation_filterRadio" value="sampletype" data-filter="Plasma"> Plasma </option> <option class="citation_filterRadio" value="sampletype" data-filter="Serum"> Serum </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="10" data-max-show="10"> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/36344536"> A multi-marker integrative analysis reveals benefits and risks of bariatric surgery </a> </span><br/> Authors: S Palleschi, V Guglielmi, L Nisticò, C Ferreri, C Tabolacci, F Facchiano, E Iorio, A Giuliani, S Brescianin, E Medda, C Fagnani, B Rossi, A Minoprio, M Chirico, ME Pisanu, F Di Nolfo, P Fortini, V Simonelli, S Baccarini, S Laterza, T Morretti, A Dell'Orso, F Manganello, P Gentilesch, P Sbraccia, E Dogliotti<br/> Scientific Reports, 2022-11-07;12(1):18877.<br/> Species: Human<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/31525609"> Transforming growth factor-beta profiles correlate with clinical symptoms and parameters of haemostasis and inflammation in a controlled human malaria infection </a> </span><br/> Authors: GM de Jong, MBB McCall, WA Dik, RT Urbanus, LJ Wammes, R Koelewijn, RW Sauerwein, A Verbon, JJ van Hellem, PJJ van Gender<br/> Cytokine, 2019-09-13;125(0):154838.<br/> Species: Human<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/30832584"> Patients with idiopathic recurrent miscarriage have abnormally high TGFï¿½+ blood NK, NKT and T cells in the presence of abnormally low TGFï¿½ plasma levels </a> </span><br/> Authors: L Zhu, M Aly, RJ Kuon, B Toth, H Wang, H Karakizlis, R Weimer, C Morath, E Ibrahim, N Ekpoom, G Opelz, V Daniel<br/> BMC Immunol., 2019-03-04;20(1):10.<br/> Species: Human<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/30391995"> Moderators for depressed mood and systemic and transcriptional inflammatory responses: a randomized controlled trial of endotoxin </a> </span><br/> Authors: MR Irwin, S Cole, R Olmstead, EC Breen, JJ Cho, M Moieni, NI Eisenberge<br/> Neuropsychopharmacology, 2018-11-03;44(3):635-641.<br/> Species: Human<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Rat" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/30390665"> ?-3 fatty acids-supplementary in gestation alleviates neuroinflammation and modulates neurochemistry in rats </a> </span><br/> Authors: M Tang, R Dang, S Liu, M Zhang, Y Zheng, R Yang, T Yin<br/> Lipids Health Dis, 2018-11-03;17(1):247.<br/> Species: Rat<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/30266090"> Cytokines in relation to hCG are significantly altered in asymptomatic women with miscarriage - a pilot study </a> </span><br/> Authors: A Freis, J Schlegel, V Daniel, J Jauckus, T Strowitzki, A Germeyer<br/> Reprod. Biol. Endocrinol., 2018-09-28;16(1):93.<br/> Species: Human<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/29529950"> The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA </a> </span><br/> Authors: AM Quinn, AR Williams, TI Sivilli, CL Raison, TWW Pace<br/> Stress, 2018-03-13;0(0):1-6.<br/> Species: Human<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/27032568"> Exposure to an inflammatory challenge enhances neural sensitivity to negative and positive social feedback </a> </span><br/> Authors: Naomi I Eisenberge<br/> Brain Behav. Immun., 2016-03-28;57(0):21-9.<br/> Species: Human<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/26215633"> Cytokines and Postoperative Delirium in Older Patients Undergoing Major Elective Surgery. </a> </span><br/> Authors: Vasunilashorn S, Ngo L, Inouye S, Libermann T, Jones R, Alsop D, Guess J, Jastrzebski S, McElhaney J, Kuchel G, Marcantonio E<br/> J Gerontol A Biol Sci Med Sci, 2015-07-27;0(0):.<br/> Species: Human<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/25939491"> Clinical Scale Zinc Finger Nuclease-mediated Gene Editing of PD-1 in Tumor Infiltrating Lymphocytes for the Treatment of Metastatic Melanoma. </a> </span><br/> Authors: Beane J, Lee G, Zheng Z, Mendel M, Abate-Daga D, Bharathan M, Black M, Gandhi N, Yu Z, Chandran S, Giedlin M, Ando D, Miller J, Paschon D, Guschin D, Rebar E, Reik A, Holmes M, Gregory P, Restifo N, Rosenberg S, Morgan R, Feldman S<br/> Mol Ther, 2015-05-05;23(8):1380-90.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> </ol> </div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/luminex-assays"> View all Luminex Assay FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="lhscm210" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay</h3> <div class="row"> <div class="col-xs-12"> <div> <div class="row"> <div class="col-md-6"> <p>There are currently no reviews for this product. 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Human TNF-alpha High Sensitivity Magnetic Luminex® Performance Assay (LHSCM210): R&D Systems