CINXE.COM

<!DOCTYPE html><html><head><title>Dark Art of Blood Cultures - Google Books</title><link rel="stylesheet" href="/books/css/_a33f2a89320471e58c940b9287b9d4eb/kl_viewport_kennedy_full_bundle.css" type="text/css" /><link rel="stylesheet"href="https://fonts.googleapis.com/css2?family=Product+Sans:wght@400"><script src="/books/javascript/v2_a33f2a89320471e58c940b9287b9d4eb__en.js"></script><script>_OC_Hooks = ["_OC_Page", "_OC_SearchReload", "_OC_TocReload", "_OC_EmptyFunc", "_OC_SearchPage", "_OC_QuotePage" ];for (var _OC_i = 0; _OC_i < _OC_Hooks.length; _OC_i++) {eval("var " + _OC_Hooks[_OC_i] + ";");}function _OC_InitHooks () {for (var i = 0; i < _OC_Hooks.length; i++) {var func = arguments[i];eval( _OC_Hooks[i] + " = func;");}}</script><link rel="canonical" href="https://books.google.com/books/about/Dark_Art_of_Blood_Cultures.html?id=_gjMDwAAQBAJ"/><meta property="og:url" content="https://books.google.com/books/about/Dark_Art_of_Blood_Cultures.html?id=_gjMDwAAQBAJ"/><meta name="title" content="Dark Art of Blood Cultures"/><meta name="description" content="In the clinical microbiology laboratory, blood is a critical diagnostic sample that, in the majority of cases is sterile (or is it?). However, when microbes gain access to and multiply in the bloodstream, it can result in life-threatening illness including sepsis. Mortality rates from bloodstream infection and sepsis range from 25% to 80%, killing millions of people annually. Blood cultures are a vital technology used in the microbiology laboratory to isolate and identify microbes and predict their response to antimicrobial therapy. The Dark Art of Blood Cultures, edited by Wm. Michael Dunne, Jr., and Carey-Ann D. Burnham, surveys the entire field of blood culture technology, providing valuable information about every phase of the process, from drawing samples to culture methods to processing positive cultures. The Dark Art of Blood Cultures is organized around several major topics. History of blood culture methods. Details the timeline of blood culture methods from manual through automated and describes the technological development of the leading automated blood culture systems (Bactec, BacT/Alert, and VersaTREK). Manual and automated blood culture methods. Critiques manual and automated methods for setting up blood cultures for adult and pediatric patients. Detection of pathogens directly from blood specimens. Describes currently available CE marked and FDA-cleared commercial tests using both phenotypic and genotypic markers, including their strengths and limitations. The workflow of culturing blood. Includes best practices from specimen collection to culture system verification, processing positive cultures for microbe identification and antibiotic susceptibility determination, along with the epidemiology of positive blood cultures and the value of postmortem blood cultures. Microorganisms in the blood. Examines the concept of a blood microbiome in healthy and diseased individuals. The Dark Art of Blood Cultures is a resource that clinicians, laboratorians, lab directors, and hospital administrators will find engaging and extremely useful. If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow. "/><meta property="og:title" content="Dark Art of Blood Cultures"/><meta property="og:type" content="book"/><meta property="og:site_name" content="Google Books"/><meta property="og:image" content="https://books.google.com.sg/books/publisher/content?id=_gjMDwAAQBAJ&printsec=frontcover&img=1&zoom=1&edge=curl&imgtk=AFLRE738hwtNqIu5qo7nvDjm8ZO0hou0grYv4Ddihl1HbDRNoCmWm6tdN6PpVhlfz5xBH9ZbFGoOSm1kmE5TsaTY3SGT6dU9sY5Umxds6mcWdRSKJWNt24BPagKGzRRb7qgKJCc4Xgus"/><link rel="image_src" href="https://books.google.com.sg/books/publisher/content?id=_gjMDwAAQBAJ&printsec=frontcover&img=1&zoom=1&edge=curl&imgtk=AFLRE738hwtNqIu5qo7nvDjm8ZO0hou0grYv4Ddihl1HbDRNoCmWm6tdN6PpVhlfz5xBH9ZbFGoOSm1kmE5TsaTY3SGT6dU9sY5Umxds6mcWdRSKJWNt24BPagKGzRRb7qgKJCc4Xgus"/><script></script><style>#gbar,#guser{font-size:13px;padding-top:1px !important;}#gbar{height:22px}#guser{padding-bottom:7px !important;text-align:right}.gbh,.gbd{border-top:1px solid #c9d7f1;font-size:1px}.gbh{height:0;position:absolute;top:24px;width:100%}@media all{.gb1{height:22px;margin-right:.5em;vertical-align:top}#gbar{float:left}}a.gb1,a.gb4{text-decoration:underline !important}a.gb1,a.gb4{color:#00c !important}.gbi .gb4{color:#dd8e27 !important}.gbf .gb4{color:#900 !important} #gbar { padding:.3em .6em !important;}</style></head><body class=""><div id=gbar><nobr><a target=_blank class=gb1 href="https://www.google.com.sg/search?tab=pw">Search</a> <a target=_blank class=gb1 href="https://www.google.com.sg/imghp?hl=en&tab=pi">Images</a> <a target=_blank class=gb1 href="https://maps.google.com.sg/maps?hl=en&tab=pl">Maps</a> <a target=_blank class=gb1 href="https://play.google.com/?hl=en&tab=p8">Play</a> <a target=_blank class=gb1 href="https://www.youtube.com/?tab=p1">YouTube</a> <a target=_blank class=gb1 href="https://news.google.com/?tab=pn">News</a> <a target=_blank class=gb1 href="https://mail.google.com/mail/?tab=pm">Gmail</a> <a target=_blank class=gb1 href="https://drive.google.com/?tab=po">Drive</a> <a target=_blank class=gb1 style="text-decoration:none" href="https://www.google.com.sg/intl/en/about/products?tab=ph"><u>More</u> »</a></nobr></div><div id=guser width=100%><nobr><span id=gbn class=gbi></span><span id=gbf class=gbf></span><span id=gbe></span><a target=_top id=gb_70 href="https://www.google.com/accounts/Login?service=print&continue=https://books.google.com.sg/books%3Fid%3D_gjMDwAAQBAJ%26q%3DIsolator%2Bsystem%26source%3Dgbs_word_cloud_r%26hl%3Den&hl=en&ec=GAZACg" class=gb4>Sign in</a></nobr></div><div class=gbh style=left:0></div><div class=gbh style=right:0></div><div role="alert" style="position: absolute; left: 0; right: 0;"><a href="https://books.google.com.sg/books?id=_gjMDwAAQBAJ&q=Isolator+system&source=gbs_word_cloud_r&hl=en&output=html_text" title="Screen reader users: click this link for accessible mode. Accessible mode has the same essential features but works better with your reader."><img border="0" src="//www.google.com/images/cleardot.gif"alt="Screen reader users: click this link for accessible mode. Accessible mode has the same essential features but works better with your reader."></a></div><div class="kd-appbar"><h2 class="kd-appname"><a href="/books">Books</a></h2><div class="kd-buttonbar left" id="left-toolbar-buttons"><a id="appbar-view-print-sample-link" href="https://books.google.com.sg/books?id=_gjMDwAAQBAJ&printsec=frontcover&vq=Isolator+system&source=gbs_vpt_read"></a><a id="appbar-view-ebook-sample-link" href="https://play.google.com/books/reader?id=_gjMDwAAQBAJ&source=gbs_vpt_read"></a><a id="appbar-patents-prior-art-finder-link" href=""></a><a id="appbar-patents-discuss-this-link" href="" data-is-grant=""></a><a id="appbar-read-patent-link" href=""></a><a id="appbar-download-pdf-link" href=""></a></div><div class="kd-buttonbar right" id="right-toolbar-buttons"></div></div><div style="display: none"><ol id="ofe-gear-menu-contents" class="gbmcc"><li class="gbe gbmtc"><a class="gbmt goog-menuitem-content" id="" href="https://www.google.com/accounts/Login?service=print&continue=https://books.google.com.sg/books%3Fop%3Dlibrary&hl=en">My library</a></li><li class="gbe gbmtc"><a class="gbmt goog-menuitem-content" id="" href="http://books.google.com.sg/support/topic/4359341?hl=en-SG">Help</a></li><li class="gbe gbmtc"><a class="gbmt goog-menuitem-content" id="" href="https://books.google.com.sg/advanced_book_search">Advanced Book Search</a></li></ol></div><div id="volume-main"><div id="volume-left"><div id=menu_container ><div id="menu_scroll_wrapper"><div id="menu_scroll" role="navigation"><div id="gb-get-book-container"><a href="https://play.google.com/store/books/details?id=_gjMDwAAQBAJ&rdid=book-_gjMDwAAQBAJ&rdot=1&source=gbs_vpt_read&pcampaignid=books_booksearch_viewport" id="gb-get-book-content">Buy eBook - SGD 81.99</a></div><p id="gb-buy-options-trigger" class="gb-buy-options-link">Get this book in print</p><h3 class=about_title><a name="buy_anchor"></a></h3><div id=buy class=about_content><div id=buy_v><ul style="list-style-type: none; padding-left: 0; margin: 0;"><li><a style="white-space:normal" href="http://www.wiley.com/remtitle.cgi?ISBN=9781683673064" dir=ltr onMouseOver="this.href='http://www.wiley.com/remtitle.cgi?ISBN\x3d9781683673064';return false" onMouseDown="this.href='/url?client\x3dca-google-print\x26format\x3dgoogleprint\x26num\x3d0\x26id\x3d_gjMDwAAQBAJ\x26q\x3dhttp://www.wiley.com/remtitle.cgi%3FISBN%3D9781683673064\x26usg\x3dAOvVaw0YqGpKUxnd0Y0KGZiASNj-\x26source\x3dgbs_buy_r';return true"><span dir=ltr>Wiley.com</span></a></li><li><a style="white-space:normal" href="http://www.amazon.com/gp/search?index=books&linkCode=qs&keywords=9781683673064" dir=ltr onMouseOver="this.href='http://www.amazon.com/gp/search?index\x3dbooks\x26linkCode\x3dqs\x26keywords\x3d9781683673064';return false" onMouseDown="this.href='/url?client\x3dca-google-print\x26format\x3dgoogleprint\x26num\x3d0\x26id\x3d_gjMDwAAQBAJ\x26q\x3dhttp://www.amazon.com/gp/search%3Findex%3Dbooks%26linkCode%3Dqs%26keywords%3D9781683673064\x26usg\x3dAOvVaw2eqf8YIAZ5hcb0LZntLW0H\x26source\x3dgbs_buy_r';return true"><span dir=ltr>Amazon.com</span></a></li><li><a style="white-space:normal" href="http://www.mphonline.com/books/nsearch.aspx?do=detail&pcode=9781683673064" dir=ltr onMouseOver="this.href='http://www.mphonline.com/books/nsearch.aspx?do\x3ddetail\x26pcode\x3d9781683673064';return false" onMouseDown="this.href='/url?client\x3dca-google-print\x26format\x3dgoogleprint\x26num\x3d0\x26id\x3d_gjMDwAAQBAJ\x26q\x3dhttp://www.mphonline.com/books/nsearch.aspx%3Fdo%3Ddetail%26pcode%3D9781683673064\x26usg\x3dAOvVaw17urD9RUXATLTIz_wabwHi\x26source\x3dgbs_buy_r';return true"><span dir=ltr>MPH</span></a></li><li><a style="white-space:normal" href="https://www.popular.com.sg/catalogsearch/result/?q=9781683673064" dir=ltr onMouseOver="this.href='https://www.popular.com.sg/catalogsearch/result/?q\x3d9781683673064';return false" onMouseDown="this.href='/url?client\x3dca-google-print\x26format\x3dgoogleprint\x26num\x3d0\x26id\x3d_gjMDwAAQBAJ\x26q\x3dhttps://www.popular.com.sg/catalogsearch/result/%3Fq%3D9781683673064\x26usg\x3dAOvVaw3XRp88VaJY5tzfXzvE5h0J\x26source\x3dgbs_buy_r';return true"><span dir=ltr>Popular</span></a></li><li><hr style="margin-right: 20%; color: #666;"></li><li><a style="white-space:normal" href="https://books.google.com.sg/url?id=_gjMDwAAQBAJ&pg=PT179&q=http://www.worldcat.org/oclc/995015970&clientid=librarylink&usg=AOvVaw0aoXpK45sUnEyLaWsFB1r8&source=gbs_buy_r"><span dir=ltr>Find in a library</span></a></li><li><a class="secondary" style="white-space:normal" href="https://books.google.com.sg/books?id=_gjMDwAAQBAJ&sitesec=buy&source=gbs_buy_r" id="get-all-sellers-link"><span dir=ltr>All sellers</span> »</a></li></ul></div></div><div class=menu id=menu><div class="menu_content" style="margin-bottom:6px"><div style="margin-bottom:4px"><div class="sidebarnav"><table border="0" cellpadding="0" cellspacing="0"><tr><td><div class="sidebarcover"><a href="https://books.google.com.sg/books?id=_gjMDwAAQBAJ&printsec=frontcover&vq=Isolator+system" onclick="_OC_Page('PA1',this.href); return false;"><img src="https://books.google.com.sg/books/publisher/content?id=_gjMDwAAQBAJ&printsec=frontcover&img=1&zoom=5&edge=curl&imgtk=AFLRE709ldPLmN-X0ga4MAt0ygQminpSeDNJMje7Md3kTVmPb4_nu395uDIHdBjrzztM3If7hxFK2_lPxi7erBKc173rLi_Hz9kyR3qjlhaZZGdkObenYKBysHem9trK0XwJInrWTmq2" alt="Front Cover" title="Front Cover" height=80 border=1 id=summary-frontcover ></a></div></td><td></td></tr></table></div><div style="clear:both"></div></div><div id="volume-info-sidebar"><h1 class="gb-volume-title" dir=ltr>Dark Art of Blood Cultures</h1><span class="addmd">edited by Wm. Michael Dunne, Jr., Carey-Ann D. Burnham</span></div><div style="margin-bottom:3px"><form action=/books id=search_form style="margin:0px;padding:0px;" method=get> <input type=hidden name="id" value="_gjMDwAAQBAJ"><table cellpadding=0 cellspacing=0 class="swv-table"><tr><td class="swv-td-search"><span><input id=search_form_input type=text maxlength=1024 class="text_flat swv-input-search" aria-label="Search in this book" name=q value="" title="Go" accesskey=i></span></td><td class="swv-td-space"><div> </div></td><td><input type=submit value="Go"></td></tr></table><script type="text/javascript">if (window['_OC_autoDir']) {_OC_autoDir('search_form_input');}</script></form></div><div><p><a id="sidebar-atb-link" href="https://books.google.com.sg/books?id=_gjMDwAAQBAJ&vq=Isolator+system&source=gbs_navlinks_s"><span dir=ltr>About this book</span></a></p></div></div></div><div><div id="navbarContainer" class="gb-navbar"></div><script>_OC_InitNavbar({"child_node":[{"title":"My library","url":"https://books.google.com.sg/books?uid=114584440181414684107\u0026source=gbs_lp_bookshelf_list","id":"my_library","collapsed":true},{"title":"My History","url":"","id":"my_history","collapsed":true}],"highlighted_node_id":""});</script><a href="/intl/en/googlebooks/tos.html" target="_blank">Terms of Service</a><h3 class=about_title><a name="pub_info_anchor"></a></h3><div id=pub_info class=about_content><div id=pub_info_v><table cellspacing=0><tr><td><a href="https://books.google.com.sg/url?id=_gjMDwAAQBAJ&pg=PT179&q=http://www.wiley.com&linkid=1&usg=AOvVaw1726WDHw7D5GGDW3B1_753&source=gbs_pub_info_r" style="text-decoration:none"><img width=175 height=35 border=0 src="https://pagead2.googlesyndication.com/pagead/imgad?id=CMvMw7qZufCXwQEQvgEYJjIIwHCbPU0c_dg" alt="John Wiley & Sons"></a><tr><td style="font-size:84.6%;color:#666666">Pages displayed by permission of <a class=link_aux href="https://books.google.com.sg/url?id=_gjMDwAAQBAJ&pg=PT179&q=http://www.wiley.com&linkid=1&usg=AOvVaw1726WDHw7D5GGDW3B1_753&source=gbs_pub_info_r">John Wiley & Sons</a>. <a style="color:#7777cc;white-space:normal" href="https://books.google.com.sg/books?id=_gjMDwAAQBAJ&printsec=copyright&vq=Isolator+system&source=gbs_pub_info_r">Copyright</a>. </table></div></div></div></div></div></div></div><div id="volume-center"><div id="scroll_atb" role="main"><div id="toolbar_container"><div style="float:left;white-space:nowrap"><table cellpadding=0 cellspacing=0><tr><td id="l_toolbar"></td><td class=toolbar-pc-cell><table cellpadding=0 cellspacing=0><tr><td class=no-jump-cell align=right><span id=page_label style="margin-right:.5em">Pages</span></td><td class=arrow style="padding-right:2px"><a href="https://books.google.com.sg/books?id=_gjMDwAAQBAJ&pg=PT178&lpg=PT179&focus=viewport&vq=Isolator+system" onclick="_OC_EmptyFunc(this.href); return false;"><div class=pagination><div id=prev_btn alt="Previous Page" title="Previous Page" class="SPRITE_pagination_v2_left"></div></div></a></td><td class=arrow><a href="https://books.google.com.sg/books?id=_gjMDwAAQBAJ&pg=PT180&lpg=PT179&focus=viewport&vq=Isolator+system" onclick="_OC_EmptyFunc(this.href); return false;"><div class=pagination><div id=next_btn alt="Next Page" title="Next Page" class="SPRITE_pagination_v2_right"></div></div></a></td></tr></table></td><td>  </td><td id=view_toolbar></td><td id=view_new></td></tr></table></div><div style="float:right"><table cellpadding=0 cellspacing=0><tr><td></td><td id="r_toolbar" style="white-space:nowrap"></td></tr></table></div><div style="clear:both"></div></div><div id="search_bar"></div><div class="gback"><div id="viewport" class="viewport" tabindex="0"><a name="page" accesskey="c"></a><table class="viewport-table" id="container" align="center" cellpadding="0" cellspacing="0"><tr><td valign="top" align="center"><noscript><style type=text/css>.imgg { width:575px;height:863px;background:#eee;padding-bottom:25px}</style><div class=imgg><div align=center><table border=0 cellpadding=0 cellspacing=0 width=500 align=center style="margin-top:2em"><tr><td rowspan=2 valign=top style="width:9px;background:#fff url('/googlebooks/bbl_l.gif') top left repeat-y"><img src="/googlebooks/bbl_tl.gif" width=9 height=7 alt=""></td><td style="background:url('/googlebooks/bbl_t.gif') top left repeat-x"><img width=1 height=7 alt=""></td><td rowspan=2 valign=top style="width:10px;background:#fff url('/googlebooks/bbl_r.gif') top right repeat-y"><img src="/googlebooks/bbl_tr.gif" width=10 height=7 alt=""></td></tr><tr><td align=center style="background:#ff9;text-align:center;line-height:1.2em"><div style="margin:1em"><img width=60 height=60 align=absmiddle src="/googlebooks/restricted_logo.gif" alt=""> <span style="font-weight:bold;font-size:1.2em"><br>Restricted Page</span></div><div style="margin:1em" align=left>You have reached your viewing limit for this book (<a href=https://books.google.com.sg/support/answer/43729?topic=9259&hl=en>why?</a>).</div></td></tr><tr><td><img src="/googlebooks/bbl_bl.gif" width=9 height=9 alt=""></td><td style="background:url('/googlebooks/bbl_b.gif') bottom left repeat-x"><img width=1 height=9 alt=""></td><td><img src="/googlebooks/bbl_br.gif" width=10 height=9 alt=""></td></tr></table></div></div></noscript></td></tr></table></div></div><script>_OC_addFlags({Host:"https://books.google.com.sg/", IsBrowsingHistoryEnabled:1, IsZipitFolderCollectionEnabled:1, IsBooksUnifiedLeftNavEnabled:1, IsBooksRentalEnabled:1});_OC_Run({"page":[{"pid":"PA1","flags":32,"order":0,"title":"1"},{"pid":"PT3","order":3},{"pid":"PT7","order":7},{"pid":"PT8","order":8},{"pid":"PT9","order":9},{"pid":"PT10","order":10},{"pid":"PT11","order":11},{"pid":"PT12","order":12},{"pid":"PT13","order":13},{"pid":"PT14","order":14},{"pid":"PT15","order":15},{"pid":"PT16","order":16},{"pid":"PT17","order":17},{"pid":"PT18","order":18},{"pid":"PT19","order":19},{"pid":"PT20","order":20},{"pid":"PT21","order":21},{"pid":"PT22","order":22},{"pid":"PT23","order":23},{"pid":"PT24","order":24},{"pid":"PT25","order":25},{"pid":"PT26","order":26},{"pid":"PT27","order":27},{"pid":"PT28","order":28},{"pid":"PT29","order":29},{"pid":"PT30","order":30},{"pid":"PT31","order":31},{"pid":"PT32","order":32},{"pid":"PT33","order":33},{"pid":"PT34","order":34},{"pid":"PT35","order":35},{"pid":"PT36","order":36},{"pid":"PT37","order":37},{"pid":"PT38","order":38},{"pid":"PT39","order":39},{"pid":"PT40","order":40},{"pid":"PT41","order":41},{"pid":"PT42","order":42},{"pid":"PT43","order":43},{"pid":"PT44","order":44},{"pid":"PT45","order":45},{"pid":"PT46","order":46},{"pid":"PT47","order":47},{"pid":"PT48","order":48},{"pid":"PT49","order":49},{"pid":"PT50","order":50},{"pid":"PT51","order":51},{"pid":"PT52","order":52},{"pid":"PT53","order":53},{"pid":"PT54","order":54},{"pid":"PT55","order":55},{"pid":"PT56","order":56},{"pid":"PT57","order":57},{"pid":"PT58","order":58},{"pid":"PT59","order":59},{"pid":"PT60","order":60},{"pid":"PT61","order":61},{"pid":"PT62","order":62},{"pid":"PT63","order":63},{"pid":"PT64","order":64},{"pid":"PT65","order":65},{"pid":"PT66","order":66},{"pid":"PT67","order":67},{"pid":"PT68","order":68},{"pid":"PT69","order":69},{"pid":"PT70","order":70},{"pid":"PT71","order":71},{"pid":"PT72","order":72},{"pid":"PT73","order":73},{"pid":"PT74","order":74},{"pid":"PT75","order":75},{"pid":"PT76","order":76},{"pid":"PT77","order":77},{"pid":"PT78","order":78},{"pid":"PT79","order":79},{"pid":"PT80","order":80},{"pid":"PT81","order":81},{"pid":"PT82","order":82},{"pid":"PT83","order":83},{"pid":"PT84","order":84},{"pid":"PT85","order":85},{"pid":"PT86","order":86},{"pid":"PT87","order":87},{"pid":"PT88","order":88},{"pid":"PT89","order":89},{"pid":"PT90","order":90},{"pid":"PT91","order":91},{"pid":"PT92","order":92},{"pid":"PT93","order":93},{"pid":"PT94","order":94},{"pid":"PT95","order":95},{"pid":"PT96","order":96},{"pid":"PT97","order":97},{"pid":"PT98","order":98},{"pid":"PT99","order":99},{"pid":"PT100","order":100},{"pid":"PT101","order":101},{"pid":"PT102","order":102},{"pid":"PT103","order":103},{"pid":"PT104","order":104},{"pid":"PT105","order":105},{"pid":"PT106","order":106},{"pid":"PT107","order":107},{"pid":"PT108","order":108},{"pid":"PT109","order":109},{"pid":"PT110","order":110},{"pid":"PT111","order":111},{"pid":"PT112","order":112},{"pid":"PT113","order":113},{"pid":"PT114","order":114},{"pid":"PT115","order":115},{"pid":"PT116","order":116},{"pid":"PT117","order":117},{"pid":"PT118","order":118},{"pid":"PT119","order":119},{"pid":"PT120","order":120},{"pid":"PT121","order":121},{"pid":"PT122","order":122},{"pid":"PT123","order":123},{"pid":"PT124","order":124},{"pid":"PT125","order":125},{"pid":"PT126","order":126},{"pid":"PT127","order":127},{"pid":"PT128","order":128},{"pid":"PT129","order":129},{"pid":"PT130","order":130},{"pid":"PT131","order":131},{"pid":"PT132","order":132},{"pid":"PT133","order":133},{"pid":"PT134","order":134},{"pid":"PT135","order":135},{"pid":"PT136","order":136},{"pid":"PT137","order":137},{"pid":"PT138","order":138},{"pid":"PT139","order":139},{"pid":"PT140","order":140},{"pid":"PT141","order":141},{"pid":"PT142","order":142},{"pid":"PT143","order":143},{"pid":"PT144","order":144},{"pid":"PT145","order":145},{"pid":"PT146","order":146},{"pid":"PT147","order":147},{"pid":"PT148","order":148},{"pid":"PT149","order":149},{"pid":"PT150","order":150},{"pid":"PT151","order":151},{"pid":"PT152","order":152},{"pid":"PT153","order":153},{"pid":"PT154","order":154},{"pid":"PT155","order":155},{"pid":"PT156","order":156},{"pid":"PT157","order":157},{"pid":"PT158","order":158},{"pid":"PT159","order":159},{"pid":"PT160","order":160},{"pid":"PT161","order":161},{"pid":"PT162","order":162},{"pid":"PT163","order":163},{"pid":"PT164","order":164},{"pid":"PT165","order":165},{"pid":"PT166","order":166},{"pid":"PT167","order":167},{"pid":"PT168","order":168},{"pid":"PT169","order":169},{"pid":"PT170","order":170},{"pid":"PT171","order":171},{"pid":"PT172","order":172},{"pid":"PT173","order":173},{"pid":"PT174","order":174},{"pid":"PT175","order":175},{"pid":"PT176","order":176},{"pid":"PT177","order":177},{"pid":"PT178","flags":8,"order":178},{"pid":"PT179","flags":8,"order":179},{"pid":"PT180","flags":8,"order":180},{"pid":"PT181","flags":8,"order":181},{"pid":"PT182","flags":8,"order":182},{"pid":"PT183","order":183},{"pid":"PT184","order":184},{"pid":"PT185","order":185},{"pid":"PT186","order":186},{"pid":"PT187","order":187},{"pid":"PT188","order":188},{"pid":"PT189","order":189},{"pid":"PT190","order":190},{"pid":"PT191","order":191},{"pid":"PT192","order":192},{"pid":"PT193","order":193},{"pid":"PT194","order":194},{"pid":"PT195","order":195},{"pid":"PT196","order":196},{"pid":"PT197","order":197},{"pid":"PT198","order":198},{"pid":"PT199","order":199},{"pid":"PT200","order":200},{"pid":"PT201","order":201},{"pid":"PT202","order":202},{"pid":"PT203","order":203},{"pid":"PT204","order":204},{"pid":"PT205","order":205},{"pid":"PT206","order":206},{"pid":"PT207","order":207},{"pid":"PT208","order":208},{"pid":"PT209","order":209},{"pid":"PT210","order":210},{"pid":"PT211","order":211},{"pid":"PT212","order":212},{"pid":"PT213","order":213},{"pid":"PT214","order":214},{"pid":"PT215","order":215},{"pid":"PT216","order":216},{"pid":"PT217","order":217},{"pid":"PT218","order":218},{"pid":"PT219","order":219},{"pid":"PT220","order":220},{"pid":"PT221","order":221},{"pid":"PT222","order":222},{"pid":"PT223","order":223},{"pid":"PT224","order":224},{"pid":"PT225","order":225},{"pid":"PT226","order":226},{"pid":"PT227","order":227},{"pid":"PT228","order":228},{"pid":"PT229","order":229},{"pid":"PT230","order":230},{"pid":"PT231","order":231},{"pid":"PT232","order":232},{"pid":"PT233","order":233},{"pid":"PT234","order":234},{"pid":"PT235","order":235},{"pid":"PT236","order":236},{"pid":"PT237","order":237},{"pid":"PT238","order":238},{"pid":"PT239","order":239},{"pid":"PT241","order":241},{"pid":"PT243","order":243},{"pid":"PT244","order":244},{"pid":"PT245","order":245},{"pid":"PT246","order":246},{"pid":"PT249","order":249},{"pid":"PT250","order":250},{"pid":"PT251","order":251},{"pid":"PT252","order":252},{"pid":"PT253","order":253},{"pid":"PT254","order":254},{"pid":"PT255","order":255},{"pid":"PT256","order":256},{"pid":"PT258","order":258},{"pid":"PT259","order":259},{"pid":"PT260","order":260},{"pid":"PT261","order":261},{"pid":"PT263","order":263},{"pid":"PT264","order":264},{"pid":"PT267","order":267},{"pid":"PT268","order":268},{"pid":"PT270","order":270},{"pid":"PT271","order":271},{"pid":"PT272","order":272},{"pid":"PT273","order":273},{"pid":"PT274","order":274},{"pid":"PT276","order":276},{"pid":"PT281","order":281},{"pid":"PT282","order":282},{"pid":"PT284","order":284},{"pid":"PT285","order":285},{"pid":"PT287","order":287},{"pid":"PT288","order":288},{"pid":"PT289","order":289},{"pid":"PT293","order":293},{"pid":"PT294","order":294},{"pid":"PT295","order":295},{"pid":"PT296","order":296},{"pid":"PT297","order":297},{"pid":"PT298","order":298},{"pid":"PT299","order":299},{"pid":"PT301","order":301},{"pid":"PT303","order":303},{"pid":"PT304","order":304},{"pid":"PT305","order":305},{"pid":"PT306","order":306},{"pid":"PT308","order":308},{"pid":"PT309","order":309},{"pid":"PT311","order":311},{"pid":"PT313","order":313},{"pid":"PT314","order":314},{"pid":"PT315","order":315},{"pid":"PT317","order":317},{"pid":"PT318","order":318},{"pid":"PT321","order":321},{"pid":"PT322","order":322},{"pid":"PT323","order":323},{"pid":"PT324","order":324},{"pid":"PT325","order":325},{"pid":"PT327","order":327},{"pid":"PT328","order":328},{"pid":"PT329","order":329},{"pid":"PT331","order":331},{"pid":"PT332","order":332},{"pid":"PT334","order":334},{"pid":"PT335","order":335},{"pid":"PT337","order":337},{"pid":"PT339","order":339},{"pid":"PT342","order":342},{"pid":"PT343","order":343},{"pid":"PT345","order":345},{"pid":"PT346","order":346},{"pid":"PT347","order":347},{"pid":"PT349","order":349},{"pid":"PT351","order":351},{"pid":"PT352","order":352},{"pid":"PT353","order":353},{"pid":"PT354","order":354},{"pid":"PT357","order":357},{"pid":"PT358","order":358},{"pid":"PT360","order":360},{"pid":"PT361","order":361},{"pid":"PT362","order":362},{"pid":"PT363","order":363},{"pid":"PT364","order":364},{"pid":"PT365","order":365},{"pid":"PT366","order":366},{"pid":"PT367","order":367},{"pid":"PT368","order":368},{"pid":"PT370","order":370},{"pid":"PT371","order":371},{"pid":"PT375","order":375},{"pid":"PT377","order":377},{"pid":"PT378","order":378},{"pid":"PT379","order":379},{"pid":"PT381","order":381},{"pid":"PT382","order":382},{"pid":"PT384","order":384},{"pid":"PT386","order":386},{"pid":"PT387","order":387},{"pid":"PT388","order":388},{"pid":"PT389","order":389},{"pid":"PT390","order":390},{"pid":"PT391","order":391},{"pid":"PT392","order":392},{"pid":"PT393","order":393},{"pid":"PT394","order":394},{"pid":"PT396","order":396},{"pid":"PT399","order":399},{"pid":"PT401","order":401},{"pid":"PT403","order":403},{"pid":"PT404","order":404},{"pid":"PT405","order":405},{"pid":"PT406","order":406},{"pid":"PT407","order":407},{"pid":"PT408","order":408},{"pid":"PT410","order":410},{"pid":"PT411","order":411},{"pid":"PT412","order":412},{"pid":"PT415","order":415},{"pid":"PT419","order":419},{"pid":"PT422","order":422},{"pid":"PT423","order":423},{"pid":"PT425","order":425},{"pid":"PT426","order":426},{"pid":"PT427","order":427},{"pid":"PT428","order":428},{"pid":"PT429","order":429},{"pid":"PT430","order":430},{"pid":"PT431","order":431},{"pid":"PT432","order":432},{"pid":"PT433","order":433},{"pid":"PT434","order":434},{"pid":"PT435","order":435},{"pid":"PT436","order":436},{"pid":"PT439","order":439},{"pid":"PT441","order":441},{"pid":"PT444","order":444},{"pid":"PT445","order":445},{"pid":"PT447","order":447},{"pid":"PT449","order":449},{"pid":"PT450","order":450},{"pid":"PT451","order":451},{"pid":"PT452","order":452},{"pid":"PT453","order":453},{"pid":"PT454","order":454},{"pid":"PT455","order":455},{"pid":"PT456","order":456},{"pid":"PT457","order":457},{"pid":"PT458","order":458},{"pid":"PT459","order":459},{"pid":"PT460","order":460},{"pid":"PT461","order":461},{"pid":"PT462","order":462},{"pid":"PT463","order":463},{"pid":"PT464","order":464},{"pid":"PT465","order":465},{"pid":"PT466","order":466},{"pid":"PT467","order":467},{"pid":"PT468","order":468},{"pid":"PT469","order":469},{"pid":"PT470","order":470},{"pid":"PT471","order":471},{"pid":"PT472","order":472},{"pid":"PT473","order":473},{"pid":"PT474","order":474},{"pid":"PT475","order":475},{"pid":"PT476","order":476},{"pid":"PT477","order":477},{"pid":"PT478","order":478},{"pid":"PT479","order":479}],"prefix":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026lpg=PT179\u0026vq=Isolator+system"},{"fullview":false,"page_width":575,"page_height":863,"font_height":16,"first_content_page":9,"disable_twopage":true,"initial_zoom_width_override":685,"show_print_pages_button":false,"title":"Dark Art of Blood Cultures","subtitle":"","attribution":"edited by Wm. Michael Dunne, Jr., Carey-Ann D. Burnham","additional_info":{"[JsonBookInfo]":{"BuyLinks":[{"Seller":"Wiley.com","Url":"http://www.wiley.com/remtitle.cgi?ISBN=9781683673064","TrackingUrl":"/url?client=ca-google-print\u0026format=googleprint\u0026num=0\u0026id=_gjMDwAAQBAJ\u0026q=http://www.wiley.com/remtitle.cgi%3FISBN%3D9781683673064\u0026usg=AOvVaw0YqGpKUxnd0Y0KGZiASNj-","IsPublisher":true},{"Seller":"Amazon.com","Url":"http://www.amazon.com/gp/search?index=books\u0026linkCode=qs\u0026keywords=9781683673064","TrackingUrl":"/url?client=ca-google-print\u0026format=googleprint\u0026num=0\u0026id=_gjMDwAAQBAJ\u0026q=http://www.amazon.com/gp/search%3Findex%3Dbooks%26linkCode%3Dqs%26keywords%3D9781683673064\u0026usg=AOvVaw2eqf8YIAZ5hcb0LZntLW0H"},{"Seller":"MPH","Url":"http://www.mphonline.com/books/nsearch.aspx?do=detail\u0026pcode=9781683673064","TrackingUrl":"/url?client=ca-google-print\u0026format=googleprint\u0026num=0\u0026id=_gjMDwAAQBAJ\u0026q=http://www.mphonline.com/books/nsearch.aspx%3Fdo%3Ddetail%26pcode%3D9781683673064\u0026usg=AOvVaw17urD9RUXATLTIz_wabwHi"},{"Seller":"Popular","Url":"https://www.popular.com.sg/catalogsearch/result/?q=9781683673064","TrackingUrl":"/url?client=ca-google-print\u0026format=googleprint\u0026num=0\u0026id=_gjMDwAAQBAJ\u0026q=https://www.popular.com.sg/catalogsearch/result/%3Fq%3D9781683673064\u0026usg=AOvVaw3XRp88VaJY5tzfXzvE5h0J"}],"AboutUrl":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ","PreviewUrl":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ","allowed_syndication_flags":{"allow_disabling_chrome":true},"online_access":{"url":"https://play.google.com/store/books/details?id=_gjMDwAAQBAJ\u0026rdid=book-_gjMDwAAQBAJ\u0026rdot=1","price":"SGD 81.99","list_price":"SGD 81.99"},"TocLine":[{"Title":"Contributors ","Pid":"PT9","PgNum":"","Order":9},{"Title":"Conventional Blood Culture Methods ","Pid":"PT44","PgNum":"","Order":44},{"Title":"LysisCentrifugation Methods of Blood Culture ","Pid":"PT70","PgNum":"","Order":70},{"Title":"Bactec Blood Culture Systems ","Pid":"PT99","PgNum":"","Order":99},{"Title":"Automation at Last in the Black ","Pid":"PT134","PgNum":"","Order":134},{"Title":"TREK Blood Culture Systems ","Pid":"PT172","PgNum":"","Order":172},{"Title":"Molecular Methods for Detection of Pathogens Directly from ","Pid":"PT207","PgNum":"","Order":207},{"Title":"Pediatric Blood Cultures ","Pid":"PT225","PgNum":"","Order":225},{"Title":"Epidemiology of Bloodstream Infections ","Pid":"PT243","PgNum":"","Order":243},{"Title":"Best Practices in Blood Cultures ","Pid":"PT271","PgNum":"","Order":271},{"Title":"Processing Positive Cultures ","Pid":"PT305","PgNum":"","Order":305},{"Title":"Fungal and Mycobacterial Blood Cultures ","Pid":"PT361","PgNum":"","Order":361},{"Title":"Postmortem Blood Culture ","Pid":"PT431","PgNum":"","Order":431}]}},"table_of_contents_page_id":"PT7","max_resolution_image_width":1280,"max_resolution_image_height":1921,"num_toc_pages":2,"quality_info":"We have no quality information about this book.","volume_id":"_gjMDwAAQBAJ","permission_info":"Pages displayed by permission of \u003ca class=link_aux href=\"https://books.google.com.sg/url?id=_gjMDwAAQBAJ\u0026pg=PT179\u0026q=http://www.wiley.com\u0026linkid=1\u0026usg=AOvVaw1726WDHw7D5GGDW3B1_753\u0026source=gbs_pub_info_r\"\u003eJohn Wiley \u0026amp; Sons\u003c/a\u003e","is_ebook":true,"volumeresult":{"has_flowing_text":true,"has_scanned_text":true,"can_download_pdf":false,"can_download_epub":false,"is_pdf_drm_enabled":false,"is_epub_drm_enabled":false},"publisher":"John Wiley \u0026 Sons","publication_date":"2017.09.01","subject":"Medical","sample_url":"https://play.google.com/books/reader?id=_gjMDwAAQBAJ\u0026source=gbs_vpt_hover","synposis":"In the clinical microbiology laboratory, blood is a critical diagnostic sample that, in the majority of cases is sterile (or is it?). However, when microbes gain access to and multiply in the bloodstream, it can result in life-threatening illness including sepsis. Mortality rates from bloodstream infection and sepsis range from 25% to 80%, killing millions of people annually. Blood cultures are a vital technology used in the microbiology laboratory to isolate and identify microbes and predict their response to antimicrobial therapy. The Dark Art of Blood Cultures, edited by Wm. Michael Dunne, Jr., and Carey-Ann D. Burnham, surveys the entire field of blood culture technology, providing valuable information about every phase of the process, from drawing samples to culture methods to processing positive cultures. The Dark Art of Blood Cultures is organized around several major topics. History of blood culture methods. Details the timeline of blood culture methods from manual through automated and describes the technological development of the leading automated blood culture systems (Bactec, BacT/Alert, and VersaTREK). Manual and automated blood culture methods. Critiques manual and automated methods for setting up blood cultures for adult and pediatric patients. Detection of pathogens directly from blood specimens. Describes currently available CE marked and FDA-cleared commercial tests using both phenotypic and genotypic markers, including their strengths and limitations. The workflow of culturing blood. Includes best practices from specimen collection to culture system verification, processing positive cultures for microbe identification and antibiotic susceptibility determination, along with the epidemiology of positive blood cultures and the value of postmortem blood cultures. Microorganisms in the blood. Examines the concept of a blood microbiome in healthy and diseased individuals. The Dark Art of Blood Cultures is a resource that clinicians, laboratorians, lab directors, and hospital administrators will find engaging and extremely useful. If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow. ","my_library_url":"https://www.google.com/accounts/Login?service=print\u0026continue=https://books.google.com.sg/books%3Fop%3Dlibrary\u0026hl=en","is_magazine":false,"is_public_domain":false,"last_page":{"pid":"PT480","order":480}},{"enableUserFeedbackUI":true,"pseudocontinuous":true,"is_cobrand":false,"sign_in_url":"https://www.google.com/accounts/Login?service=print\u0026continue=https://books.google.com.sg/books%3Fid%3D_gjMDwAAQBAJ%26q%3DIsolator%2Bsystem%26source%3Dgbs_word_cloud_r%26hl%3Den\u0026hl=en","isEntityPageViewport":false,"showViewportOnboarding":false,"showViewportPlainTextOnboarding":false},{"page":[{"pid":"PT179","flags":8,"order":179,"vq":"Isolator system"}]},null,{"number_of_results":57,"search_results":[{"page_id":"PT35","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e and was initially marketed by E. I. du Pont de Nemours \u0026amp; Co., Wilmington, DE, and later by Wampole Laboratories, Cranbury, NJ, and is reviewed in much greater detail by McElvania TeKippe and Pence in\u0026nbsp;..."},{"page_id":"PT36","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e led to the commercialization of the product known as the \u003cb\u003eIsolator\u003c/b\u003e (DuPont, then Wampole). Reproduced from ... \u003cb\u003esystem\u003c/b\u003e. The ARD consisted of a 60-ml rubber septum-capped vial containing 10 g of a cationic resin (C249-Ionac\u0026nbsp;..."},{"page_id":"PT72","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e lysis- centrifugation blood culture \u003cb\u003esystem\u003c/b\u003e (Wampole Laboratories, Cranbury, NJ) is used to enhance detection of a subset of bacterial pathogens, molds (especially dimorphic fungi), and slowly growing Mycobacterium spp\u0026nbsp;..."},{"page_id":"PT73","snippet_text":"... Isostat Microbial \u003cb\u003eSystem\u003c/b\u003e includes a 10-ml glass vacutainer tube, the \u003cb\u003eIsolator\u003c/b\u003e 10, which includes the following additives: (i) saponin, a lysing agent; (ii) polypropylene glycol, an antifoaming agent; (iii) anticoagulants SPS and\u0026nbsp;..."},{"page_id":"PT74","snippet_text":"... \u003cb\u003eSystem\u003c/b\u003e is as follows: first, 10 ml of blood must be placed in the provided \u003cb\u003eIsolator\u003c/b\u003e 10 vacuum tube. Volumes of \u0026lt;10 ml are problematic because the increased SPS concentration in a less than full tube is potentially toxic to fastidious\u0026nbsp;..."},{"page_id":"PT75","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e 10 septum (Fig. 2). A specialized pipette is used to remove the supernatant from the \u003cb\u003eIsolator\u003c/b\u003e tube, after ... \u003cb\u003esystem\u003c/b\u003e is manual and not automated, once the specimen is plated to solid media, the plates must be viewed one to\u0026nbsp;..."},{"page_id":"PT78","snippet_text":"... Isolator 10 use. Benefits and Drawbacks The benefit of the \u003cb\u003eIsolator system\u003c/b\u003e is that it requires very little equipment beyond a centrifuge. This allows testing to be performed in laboratories that do not have the resources to support an\u0026nbsp;..."},{"page_id":"PT79","snippet_text":"... Isolator 10 tubes showed that the \u003cb\u003eIsolator system\u003c/b\u003e allowed for greater and more rapid detection of staphylococci , Enterobacteriaceae , and yeasts , but recovered fewer streptococci and anaerobes ( 13–16 ) . With the evolution of CMBCSs\u0026nbsp;..."},{"page_id":"PT81","snippet_text":"... Isolator tubes have been used to recover Bartonella spp. from blood cultures, the preferred diagnostic methods for Bartonella infection are serology and/or PCR (32). Isolator tubes also ... \u003cb\u003eIsolator system\u003c/b\u003e in cases of polymicrobial."},{"page_id":"PT82","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e detected 21 of 25 polymicrobial infections, while 17 were detected by standard blood cultures (38). A second study showed that the \u003cb\u003eIsolator system\u003c/b\u003e detected 21 episodes of polymicrobial bacteremia, while blood culture\u0026nbsp;..."},{"page_id":"PT83","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e tubes can be held at room temperature for 16 h without impacting organism viability , Stockman et al ... \u003cb\u003esystem\u003c/b\u003e has demonstrated better recovery of dimorphic and filamentous fungi than CMBCSs , especially Histoplasma\u0026nbsp;..."},{"page_id":"PT84","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e 1.5 \u003cb\u003esystem\u003c/b\u003e compared with Bactec FX and BacT/Alert CMBCSs for detection of fungemia in children. Over ten years, 9,442 specimens were processed with yeast or yeast-like organisms recovered in 297 (3.1%) specimens (151 clinical\u0026nbsp;..."},{"page_id":"PT85","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e provided supporting evidence, but not the sole diagnosis, of disseminated infection. The later four fungi were recovered solely by the \u003cb\u003eIsolator system\u003c/b\u003e. Isolation of Acid-Fast Bacilli Recovery of mycobacteria from blood\u0026nbsp;..."},{"page_id":"PT86","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e blood culture resulted in organism detection in 60 to 65 h, while only 10 of 34 concurrently drawn ... \u003cb\u003esystem\u003c/b\u003e to identify M. tuberculosis from the blood of a patient with infection of the lungs, pleural cavity, and breast\u0026nbsp;..."},{"page_id":"PT87","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e but only one was detected by BACTEC 13A (66). On average, BACTEC 13A detected mycobacterial bacteremia two days sooner than the \u003cb\u003eIsolator system\u003c/b\u003e. A 2002 study comparing Isolator blood culture and BACTEC 13A with CMBCSs\u0026nbsp;..."},{"page_id":"PT88","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e with the sediment pellet being incubated either directly to M7H11 solid media or inoculated into Bactec 12B broth media had similar sensitivity to blood directly incubated in specially formulated Bactec 13A mycobacterial\u0026nbsp;..."},{"page_id":"PT89","snippet_text":"... Isolator cultures is not routinely performed. An advantage of the Isolator is that it can be used in low- resource settings to culture bacteria, fungi, and AFB from blood. A study from Malawi in the year 2000 used the \u003cb\u003eIsolator system\u003c/b\u003e in\u0026nbsp;..."},{"page_id":"PT90","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e is used primarily for detection of slowly growing Mycobacterium spp. and fungi, especially dimorphic fungi. For bacteria, it may have utility in detection of Bartonella, F. magna, and P. anaerobius. In the future, the\u0026nbsp;..."},{"page_id":"PT91","snippet_text":"... \u003cb\u003eSystem\u003c/b\u003e , Package Insert IN - 050C1-03 . Cashman JS , Boshard R , Matsen JM . 1983. Viability of organisms held in the \u003cb\u003eisolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e for 15 h and their rapid detection by acridine orange staining . J Clin Microbiol 18\u0026nbsp;..."},{"page_id":"PT92","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e with BACTEC radiometric blood culture media . J Clin Microbiol 20 : 618–623 . 17 ... \u003cb\u003eisolator\u003c/b\u003e and BacT / Alert aerobic blood culture \u003cb\u003esystems\u003c/b\u003e . J Clin Microbiol 33 : 1787- 1790 . 19. Pickett DA , Welch\u0026nbsp;..."},{"page_id":"PT95","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e aerobic blood culture \u003cb\u003esystems\u003c/b\u003e . Am J Clin Pathol 105 : 774–781 . 44. Gander RM , Byrd L , DeCrescenzo M , Hirany S , Bowen M , Baughman J. 2009. Impact of blood cultures drawn by phlebotomy on contamination rates and health\u0026nbsp;..."},{"page_id":"PT96","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e for detection of fungemia and bacteremia . J Clin Microbiol 31 : 865–871 . 56. Murray PR . 1991. Comparison of the lysis - centrifugation and agitated biphasic blood culture \u003cb\u003esystems\u003c/b\u003e for detection of\u0026nbsp;..."},{"page_id":"PT97","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e lysis- centrifugation blood culture \u003cb\u003esystem\u003c/b\u003e . J Clin Microbiol 21 : 647–648 . 65. Agy MB , Wallis CK , Plorde JJ , Carlson LC , Coyle MB . 1989. Evaluation of four mycobacterial blood culture media : BACTEC 13A , \u003cb\u003eIsolator\u003c/b\u003e\u0026nbsp;..."},{"page_id":"PT98","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e. J Clin Microbiol 29:1211–1214. Roth RI, Owen RL, Keren DF, Volberding PA. 1985 ... \u003cb\u003eISOLATOR\u003c/b\u003e 10 (lysis-centrifugation) \u003cb\u003esystems\u003c/b\u003e for detection of bacteremia, mycobacteremia, and fungemia in a developing\u0026nbsp;..."},{"page_id":"PT110","snippet_text":"... Isolator tubes were filled with 10 ml of blood, and, following centrifugation and processing, the sediment was inoculated onto aerobic and anaerobic media. The \u003cb\u003eIsolator system\u003c/b\u003e detected significantly more pathogens than either 3-ml (P\u0026nbsp;..."},{"page_id":"PT111","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e and 5-ml anaerobic Bactec bottle may provide optimal comprehensive recovery of blood-borne pathogens. The Bactec 460 \u003cb\u003esystem\u003c/b\u003e was associated with episodes of pseudobacteremia, with one laboratory reporting 23 cases\u0026nbsp;..."},{"page_id":"PT121","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e, was conducted in a population of patients with AIDS and suspected fungemia or mycobacteremia (36). Seven hundred seventeen cultures were performed, consisting of 5 ml of blood in the Bactec MYCO/F Lytic bottle, and 6 to\u0026nbsp;..."},{"page_id":"PT122","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e. The time to detection for H. capsulatum did not differ between the two systems, but time to identification was shorter from the \u003cb\u003eIsolator system\u003c/b\u003e, because colonies on solid media were available earlier. Conversely, C\u0026nbsp;..."},{"page_id":"PT123","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e or laboratory-developed methods with plating on mycobacterial solid media, was the conventional culture method. Bactec media 12B allowed concentrate to also be inoculated into broth media and analyzed by the radiometric\u0026nbsp;..."},{"page_id":"PT124","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e (P \u0026lt; 0.001), and shorter for BacT/Alert MB (11.0 days) than for MYCO/F Lytic (12.8 days) (P = 0.038). Interestingly, of the five MTB isolates, no single \u003cb\u003esystem\u003c/b\u003e detected all; \u003cb\u003eIsolator\u003c/b\u003e and 13A each recovered 80%, while MYCO/F\u0026nbsp;..."},{"page_id":"PT130","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e with BAАСТЕС radiometric blood culture media . J Clin Microbiol 20 : 618–623 . 15. Craven DE , Lichtenberg DA , Browne KF , Coffey DM , Treadwell TL , McCabe WR . 1984. Pseudobacteremia traced to cross\u0026nbsp;..."},{"page_id":"PT132","snippet_text":"... \u003cb\u003esystems\u003c/b\u003e . J Clin Microbiol 52 : 3558–3567 . 31. Miller NS , Rogan D , Orr BL , Whitney D. 2011. Comparison of BD ... \u003cb\u003eisolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e for detection of fungemia in children : a 10 - year review . J Clin Microbiol 54\u0026nbsp;..."},{"page_id":"PT133","snippet_text":"... \u003cb\u003eISOLATOR\u003c/b\u003e 10 \u003cb\u003esystems\u003c/b\u003e for detection of mycobacteremia . J Clin Microbiol 41 : 1987–1990 . 41. Fuller DD , Davis TE ... \u003cb\u003esystem\u003c/b\u003e for culturing miscellaneous sterile body fluids . Res Microbiol 157 : 433–436 . 43. Yagupsky P , Dagan R\u0026nbsp;..."},{"page_id":"PT179","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e. Similar to findings in the Morello study, the ESP \u003cb\u003esystem\u003c/b\u003e detected more clinically significant organisms than the BacT/Alert \u003cb\u003esystem\u003c/b\u003e (86 versus 47). Several publications compared the ESP with the \u003cb\u003eIsolator\u003c/b\u003e (Alere, Waltham, MA), a\u0026nbsp;..."},{"page_id":"PT180","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e. However, the \u003cb\u003eIsolator\u003c/b\u003e/Thiol combination resulted in a contamination rate greater than six times that of the ESP \u003cb\u003esystem\u003c/b\u003e. A third study performed by Cockerill et al. compared the ESP, \u003cb\u003eIsolator\u003c/b\u003e, and Becton Dickinson Septi-Chek\u0026nbsp;..."},{"page_id":"PT202","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e for detection of bacteremia in neonates . J Clin Microbiol 25 : 2098–2101 . 3 . 2. Roberts G , Kaczmarski E ... \u003cb\u003eIsolator\u003c/b\u003e and ESP aerobic blood culture \u003cb\u003esystems\u003c/b\u003e for detection of bloodstream infections . J Clin Microbiol 32\u0026nbsp;..."},{"page_id":"PT203","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e , and Becton Dickinson Septi - Chek aerobic blood culturing \u003cb\u003esystems\u003c/b\u003e . J Clin Microbiol 34 : 20–24 . 12. Tinghitella TJ , Lamagdeleine MD . 1995. Assessment of Difco ESP 384 blood culture \u003cb\u003esystem\u003c/b\u003e by terminal subcultures : failure\u0026nbsp;..."},{"page_id":"PT267","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e for detection of fungemia in children : a 10 - year review . J Clin Microbiol 54 : 2284–2287 . 62. Kauffman CA. 2007. Histoplasmosis : a clinical and laboratory update . Clin Microbiol Rev 20 : 115–132 . 63\u0026nbsp;..."},{"page_id":"PT347","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e aerobic culture \u003cb\u003esystem\u003c/b\u003e for detection of bloodstream infections. J Clin Microbiol 35:1469–1472. 10. 11. 12 ... \u003cb\u003esystems\u003c/b\u003e. J Med Microbiol 55:53–57. Ziegler R, Johnscher I, Martus P, Lenhardt D, Just HM. 1998. Controlled\u0026nbsp;..."},{"page_id":"PT366","snippet_text":"... \u003cb\u003eSystems\u003c/b\u003e, Sparks, MD) and lysis-centrifugation \u003cb\u003esystems\u003c/b\u003e (Wampole Isostat/\u003cb\u003eIsolator\u003c/b\u003e Microbial \u003cb\u003eSystem\u003c/b\u003e; Alere, Waltham, MA) have both been used to recover dimorphic and filamentous fungi. Poor performance of the biphasic culture \u003cb\u003esystem\u003c/b\u003e has\u0026nbsp;..."},{"page_id":"PT367","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e lysis-centrifugation \u003cb\u003esystem\u003c/b\u003e over other blood culture methods for fungal detection is with Histoplasma capsulatum. This advantage extends to both a higher sensitivity for detecting H. capsulatum (30–32) and a shorter time to\u0026nbsp;..."},{"page_id":"PT370","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e lysis-centrifugation blood culture (31). On retrospective review of matched blood cultures, Lyon and Woods (32) observed that growth of 64 clinical isolates ... \u003cb\u003eIsolator\u003c/b\u003e lysis-centrifugation \u003cb\u003esystem\u003c/b\u003e and plated onto fungal media."},{"page_id":"PT371","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e lysis-centrifugation \u003cb\u003esystem\u003c/b\u003e and plated onto fungal media (47). Some species of filamentous fungi like Scedosporium and Fusarium can grow within 3 to 5 days, but, if infection is suspected, they should be incubated for an\u0026nbsp;..."},{"page_id":"PT379","snippet_text":"... \u003cb\u003eIsolator system\u003c/b\u003e (Wampole Laboratories, Cranbury, NJ) and the Bactec 13A blood culture bottle (Becton Dickinson Microbiology Systems, Sparks, MD). Whereas the \u003cb\u003eIsolator system\u003c/b\u003e is still commonly used in the mycobacteriology laboratory, the\u0026nbsp;..."},{"page_id":"PT381","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e 10 \u003cb\u003esystem\u003c/b\u003e, or liquid media with either manual, intermittent or automated, continuous detection of growth. Historically, mycobacterial blood culture in liquid media used manual, intermittent detection of growth in radiometric\u0026nbsp;..."},{"page_id":"PT389","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e . J Clin Microbiol 42 : 773–777 . Horvath LL , George BJ , Murray CK , Harrison LS , Hospenthal DR . 2004 ... \u003cb\u003eIsolator\u003c/b\u003e and Roche Septi - Chek for detection of fungemia . J Clin Microbiol 25 : 1623–1625 . Kauffman CA. 2007\u0026nbsp;..."},{"page_id":"PT391","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e . Diagn Microbiol Infect Dis 72 : 328–331 . 23. Zheng S , Ng TY , Li H , Tan AL , Tan TT , Tan BH . 2016. A ... \u003cb\u003eisolator\u003c/b\u003e blood cultures . J Clin Microbiol 38 : 3827–3829 . 28. McDonald LC , Weinstein MP , Fune J , Mirrett S\u0026nbsp;..."},{"page_id":"PT392","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e blood culture \u003cb\u003esystem\u003c/b\u003e for detection of fungemia and bacteremia . J Clin Microbiol 31 : 865–871 . 32. Lyon R , Woods G. 1995. Comparison of the BacT / Alert and \u003cb\u003eIsolator\u003c/b\u003e blood culture \u003cb\u003esystems\u003c/b\u003e for recovery of fungi . Am J Clin\u0026nbsp;..."},{"page_id":"PT393","snippet_text":"... \u003cb\u003eisolator\u003c/b\u003e and BACTEC 660 resin media for blood culture . J Clin Microbiol 28 : 1925–1927 . 41. Horvath LL , George BJ , Hospenthal DR . 2007. Detection of fifteen species of Candida in an automated blood culture \u003cb\u003esystem\u003c/b\u003e . J Clin Microbiol\u0026nbsp;..."},{"page_id":"PT401","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e , Septi - Chek AFB \u003cb\u003esystem\u003c/b\u003e , and Lowenstein - Jensen medium for detection of mycobacteria . J Clin Microbiol ... \u003cb\u003eISOLATOR\u003c/b\u003e 10 ( lysis - centrifugation ) \u003cb\u003esystems\u003c/b\u003e for detection of bacteremia , mycobacteremia , and fungemia in a\u0026nbsp;..."},{"page_id":"PT449","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e , 99 Akzo Nobel , 87 Alcaligenes ( Achromobacter ) xylosoxidans , recovery with \u003cb\u003eIsolator\u003c/b\u003e , 118 Alzheimer\u0026#39;s disease , 283–287 inflammatory component , 285 , 289 lipopolysaccharide ( LPS ) , 286–288 pathology , 284 research , 289\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT449\u0026vq=Isolator+system"},{"page_id":"PT457","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e , 118 VersaTREK \u003cb\u003esystem\u003c/b\u003e , 132 Candida glabrata , 74 , 75 fungal bloodstream infections , 168 , 168–169 fungemia , 246 , 247 recovery with \u003cb\u003eIsolator\u003c/b\u003e , 118 Candida krusei , 74 , 75 , 168 , 168–169 Candida parapsilopsis , 74 , 75\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT457\u0026vq=Isolator+system"},{"page_id":"PT459","snippet_text":"... system , 117–118 , 119 comparison to \u003cb\u003eIsolator system\u003c/b\u003e , 118 media types , 116 mycobacterial culture , 259 performance of , 117 photograph , 115 workflow , 116–117 see also TREK blood culture systems Difco Laboratories , 113 , 115\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT459\u0026vq=Isolator+system"},{"page_id":"PT460","snippet_text":"... \u003cb\u003esystem\u003c/b\u003e, 119 pediatric bloodstream infection, 167 postmortem blood culture, 305 recovery from blood culture, 173, 174 recovery with \u003cb\u003eIsolator\u003c/b\u003e, 118 vancomycin-resistant, 187 VersaTREK \u003cb\u003esystem\u003c/b\u003e, 126 Enterococcus avium, detection assay, 217\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT460\u0026vq=Isolator+system"},{"page_id":"PT464","snippet_text":"... \u003cb\u003eIsolator\u003c/b\u003e microbial tubes comparison to Difco ESP \u003cb\u003esystem\u003c/b\u003e , 118 isolation of acid - fast bacilli , 49–52 isolation of bacteria , 45–47 isolation of fungi , 47–49 mycobacterial blood culture , 258 , 259 , 266 uses for , 45–52 J Johns\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT464\u0026vq=Isolator+system"},{"page_id":"PT475","snippet_text":"... \u003cb\u003eSystem\u003c/b\u003e , 71 , 113–114 Solid organ transplantation ( SOT ) , 163 , 170 Sphingobacteria , 282 Sporosarcina globispora , 281 ST . See ... \u003cb\u003eIsolator\u003c/b\u003e , 118 \u003cb\u003esystem\u003c/b\u003e comparisons , 102–104 VersaTREK \u003cb\u003esystem\u003c/b\u003e , 128 Staphylococcus epidermidis.","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT475\u0026vq=Isolator+system"},{"page_id":"PT479","snippet_text":"... \u003cb\u003esystems\u003c/b\u003e Vibrio spp . , 208 , 211 Villemin , Jean Antoine , 4 , 4 Volume , blood collection , 22 , 184 VYOO assay ... \u003cb\u003eSystem\u003c/b\u003e , 41 , 42 , 43 , 249 X Xanthomonas ( Stenotrophomonas ) maltophilia , recovery with \u003cb\u003eIsolator\u003c/b\u003e , 118 Y Yeast\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT479\u0026vq=Isolator+system"}],"search_query_escaped":"Isolator system"},{});</script></div></div></div><script>(function() {var href = window.location.href;if (href.indexOf('?') !== -1) {var parameters = href.split('?')[1].split('&');for (var i = 0; i < parameters.length; i++) {var param = parameters[i].split('=');if (param[0] == 'focus') {var elem = document.getElementById(param[1]);if (elem) {elem.focus();}}}}})();</script>