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}) })(jQuery); </script> </head> <body class="post-template-default single single-post postid-134 single-format-standard header-image full-width-content genesis-breadcrumbs-visible"><div class="site-container"><header class="site-header"><div class="wrap"><div class="title-area"><p class="site-title"><a href="https://www.thermofisher.com/" title="Thermo Fisher Scientific">Thermo Fisher Scientific</a></p><p class="site-description">Electron and cryo-electron microscopy stories and solutions about the advancement of cellular and structural biology</p></div><div class="widget-area header-widget-area"><section id="block-5" class="widget widget_block"><div class="widget-wrap"> <div class="wp-block-group"><div class="wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow"></div></div> </div></section> <section id="block-6" class="widget widget_block"><div class="widget-wrap"> <div class="wp-block-group"><div class="wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow"></div></div> </div></section> </div><div class="breadcrumb"><span class="breadcrumb-link-wrap"><a href="https://www.thermofisher.com/blog/?">Accelerating Science</a></span><span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/atomic-resolution/"><span class="breadcrumb-link-text-wrap">Life in Atomic Resolution</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/atomic-resolution/cryo-electron-microscopy-cryo-em/"><span class="breadcrumb-link-text-wrap">Cryo-Electron Microscopy (Cryo-EM)</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-current-title">Cryo-EM in the Classroom</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-134 post type-post status-publish format-standard has-post-thumbnail category-cryo-electron-microscopy-cryo-em category-electron-microscopy-101 tag-3d tag-columbia-university tag-cryo-electron-microscopy tag-cryo-em tag-electron-microscopy tag-fei tag-mrc-laboratory-of-molecular-biology tag-purdue-university tag-thermo-fisher-scientific tag-thermo-scientific tag-university-of-san-diego entry" aria-label="Cryo-EM in the Classroom"><header class="entry-header"><h1 class="entry-title">Cryo-EM in the Classroom</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/laurenshaber/" title="Posts by Lauren Shaber" rel="author">Lauren Shaber</a> </span> <span class="post-info-date"> 11.07.2018 </span> </header><div class="entry-content"><p><em>Scientists at academic institutions continue to work on the cutting-edge of cryo-EM research. To celebrate the work these scientists are doing to solve some of the world’s most complex problems, we’re hosting a “Cryo-EM in the Classroom” series to spotlight the latest research in academia. </em></p> <p>Using <a href="https://ter.li/g807lv">cryo-electron microscopy</a> (cryo-EM), today’s scientists aren’t limited to low-resolution 2D images but instead have the ability to image high-resolution 3D structures. By studying these 3D models, researchers gain a better understanding of the structure and interactions of certain proteins at the atomic level — the same level of resolution that physicists use to study beams of light. Ultimately, these new insights can speed the path to more effective treatments against viruses and diseases.</p> <div style="width: 510px" class="wp-caption aligncenter"><img decoding="async" src="https://www.thermofisher.com/blog/atomic-resolution/wp-content/uploads/sites/22/2022/04/cryoem-1.jpg" width="500" height="500" /><p class="wp-caption-text">Detailed reconstructions are now available with modern cryo-EM. 5N8Y. KaiCBA circadian clock backbone model based on a Cryo-EM density via DOI: 10.1126/science.aag3218</p></div> <p>For example, Dr. Timothy Baker from the University of San Diego Division of Biological Sciences has applied cryo-EM in his studies for over two decades. Dr. Baker uses cryo-EM instruments to create 3D structures to better understand the <a href="https://ter.li/y4xa95">interactions a virus has with a host cell as it replicates</a>. In one such study, Dr. Baker used cryo-EM to visualize how <a href="https://ter.li/rkzpl8">different proteins latch onto a bacterial host</a>, and how the tail spike protein changes conformations to facilitate infection as shown here.</p> <p>Just this year, researchers at Purdue University leveraged cryo-EM to <a href="https://ter.li/1b9783">map out the complexities of the Zika virus</a> and show the structure of a mature Zika virus at 3.1Å resolution. Two years prior, those same researchers were only able to produce a resolution of 3.8Å. With new virus preparation methods and updated data processing techniques, the team was able to refine the quality of their structures. Access to this detailed 3D structure of the Zika virus equips researchers with crucial information in their move to design more effective and targeted vaccines.</p> <div style="width: 2590px" class="wp-caption alignnone"><img decoding="async" src="https://www.thermofisher.com/blog/atomic-resolution/wp-content/uploads/sites/22/2022/04/rossmann-zika-1-scaled.jpg" alt="Cryo-EM structure of mature Zika virus at 3.1Å resolution." width="2580" height="2449" /><p class="wp-caption-text">Cryo-EM structure of mature Zika virus at 3.1Å resolution. (Purdue University photo/Madhumati Sevvana)</p></div> <p>Alzheimer’s is a neurological epidemic that has been studied for over a century. Over time, and often later in life, the neurodegenerative disease causes memory loss and impaired thinking, eventually leading to the inability to carry out simple tasks. In the late 1980s, scientists learned that tau protein was a key component of the disease, but <a href="https://ter.li/9431zg">it wasn’t until 2017</a> that a team of researchers, led by Dr. Sjors Scheres and Dr. Michel Goedert of <a href="https://ter.li/98ipn6" target="_blank" rel="noopener noreferrer">MRC Laboratory of Molecular Biology</a>, <a href="https://ter.li/ne356g">visualized the filaments’ atomic structure</a>. Dr. Anthony Fitzpatrick, who participated in the 2017 research, and his group at Columbia University’s <a href="https://ter.li/j4wiy0">Zuckerman Institute</a> <a href="https://ter.li/jz8i5n">continue to bring new voices to the table</a> to decipher the disease, in hopes that their research will lay foundations for future breakthroughs.</p> <div style="width: 2491px" class="wp-caption alignnone"><img loading="lazy" decoding="async" src="https://www.thermofisher.com/blog/atomic-resolution/wp-content/uploads/sites/22/2022/04/cryoem20columbia-1-scaled.jpg" width="2481" height="2658" /><p class="wp-caption-text">Tau filaments as imaged with cryo-EM. Credit: Anthony Fitzpatrick/Columbia’s Zuckerman Institute</p></div> <p>Stay tuned for more posts in this <em>Cryo-EM in the Classroom</em> series where we’ll profile amazing cryo-EM work from researchers at academic and research institutions around the globe. In the meantime, check out <a href="https://ter.li/tcynnm">EM-Learning.com</a>, an online learning center where you can access more than 50 hours of lectures and videos on all things cryo-EM – at no cost to you!</p> <p><em>Do you know someone who should be featured for their cryo-EM work? Reach out to us on <a href="https://ter.li/si1jtw">Twitter</a> or <a href="https://ter.li/hy349t">LinkedIn</a>. <a href="https://www.thermofisher.com/blog/subscribe/">Subscribe </a>to receive Accelerating Microscopy updates straight to your inbox.</em></p> <p>To learn more about cryo-EM, <a href="https://ter.li/jjzfwz">fill out this form</a> to speak with an expert.</p>  <div class="jp-relatedposts"> <div class="related-post related-1"> <a href="https://www.thermofisher.com/blog/atomic-resolution/what-is-cryo-em-what-is-cryo-electron-microscopy/"><img width="310" height="274" src="https://www.thermofisher.com/blog/atomic-resolution/wp-content/uploads/sites/22/2024/10/cryoEM-blog-virus-pore4-1920x600_Header-310x274.jpg" class="attachment-grid size-grid wp-post-image" alt="cryoEM-blog-virus pore" decoding="async" loading="lazy" /></a> <span class="grid-content"> <h4 class="entry-title"><a 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