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}) })(jQuery); </script> </head> <body class="post-template-default single single-post postid-716 single-format-standard header-image header-full-width full-width-content genesis-breadcrumbs-hidden"><div class="site-container"><header class="site-header"><div class="wrap"><div class="title-area"><p class="site-title"><a href="https://www.thermofisher.com/" title="Thermo Fisher Scientific">Thermo Fisher Scientific</a></p><p class="site-description">Molecular testing content for clinical lab professionals and clinicians</p></div></div></header> <nav role="navigation" class="top-menu tm" style=""> <ul> <li><a href="#" aria-haspopup="true">Categories<div data-uw-styling-context="true"><b data-uw-styling-context="true"></b></div></a> <ul class="dropdown" aria-label="submenu"> <li><a href=" https://www.thermofisher.com/blog/clinical-conversations/science-and-education/">Science and education</a></li> <li><a href=" https://www.thermofisher.com/blog/clinical-conversations/product-news/">Product news</a></li> <li><a href=" https://www.thermofisher.com/blog/clinical-conversations/case-studies/">Case studies</a></li> <li><a href=" https://www.thermofisher.com/blog/clinical-conversations/perspectives/">Perspectives</a></li> <li><a href=" https://www.thermofisher.com/blog/clinical-conversations/events/">Events</a></li> <li><a href=" https://www.thermofisher.com/blog/clinical-conversations/news/">News</a></li> </ul> </li> </ul> </nav> <div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-716 post type-post status-publish format-standard has-post-thumbnail category-science-and-education tag-content-video tag-covid-19 tag-mutations tag-pcr tag-sars-cov-2 entry" aria-label="The SARS-CoV-2 Mutation Panel: How It Works"><header class="entry-header"><h1 class="entry-title">The SARS-CoV-2 Mutation Panel: How It Works</h1> <script type="text/javascript"> function cc_toggle(id){ var s = document.getElementById(id); if(isHidden(s)){ s.style.display = 'inline'; } else { s.style.display = 'none'; } } function isHidden(element){ var style = window.getComputedStyle(element); return (style.display === 'none'); } </script> <span class="cc_sidebar"> <span class="cc_color_bar"> <span class="cc_home_icon"> <a href="https://www.thermofisher.com/blog/clinical-conversations/"><img src="https://www.thermofisher.com/blog/clinical-conversations/wp-content/themes/clinical-conversations/images/icon_home.png" alt="Clinical Conversation blog - home icon" /></a> </span> <span class="cc_post_category"> <a class='cc_colorbar_category' href='https://www.thermofisher.com/blog/clinical-conversations/category/science-and-education/'>Science and education</a> </span>  <span id="cc_post_readtime"> 2 mins. </span> <span class="cc_toggle_social"> <a href="javascript:cc_toggle('cc_jetpack_shares');">Sharing ▼</a> </span> <span class="cc_toggle"> <a href="javascript:cc_toggle('cc_metadata');">Details ▼</a> </span> </span> </span> <span id="cc_jetpack_shares"> <ul> <li><a target="_blank" href='https://www.linkedin.com/shareArticle?mini=true&url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fclinical-conversations%2Fsars-cov-2-mutation-panel-how-it-works%2F&title=The+SARS-CoV-2+Mutation+Panel%3A+How+It+Works'><img src="https://www.thermofisher.com/blog/clinical-conversations//wp-content/themes/clinical-conversations/images/social_icons_linkedin.jpg" alt="Linked In sharing icon" /></a></li> <li><a target="_blank" href='https://www.facebook.com/sharer/sharer.php?u=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fclinical-conversations%2Fsars-cov-2-mutation-panel-how-it-works%2F'><img src="https://www.thermofisher.com/blog/clinical-conversations//wp-content/themes/clinical-conversations/images/social_icons_facebook.jpg" alt="Facebook sharing icon" /></a></li> <li><a target="_blank" href='https://twitter.com/intent/tweet?text=The+SARS-CoV-2+Mutation+Panel%3A+How+It+Works&url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fclinical-conversations%2Fsars-cov-2-mutation-panel-how-it-works%2F&'><img src="https://www.thermofisher.com/blog/clinical-conversations//wp-content/themes/clinical-conversations/images/social_icons_twitter.jpg" alt="Twitter sharing icon" /></a></li> <li><a href='mailto:?subject=You might be interested in The SARS-CoV-2 Mutation Panel: How It Works from the Clinical Conversations blog.&body=You might be interested in The SARS-CoV-2 Mutation Panel: How It Works from the Clinical Conversations blog at https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fclinical-conversations%2Fsars-cov-2-mutation-panel-how-it-works%2F'><img src="https://www.thermofisher.com/blog/clinical-conversations/wp-content/themes/clinical-conversations/images/icon_email.png" alt="Email sharing icon" /></a></li> </ul> </span> <span id="cc_metadata"> <span class="cc_dateline"> <span class="post-info-date">  30 Jul 2021 </span> || <span class="post-info-author">   By Peter Friebe </span> </span> <span class="cc_shares"> <span class="cc_shares_subhead" >Shares: <span class="share_count">0</span> </span> </span> <span class="cc_version"> <span class="cc_version_subhead">Versions of this article</span> Original article. </span> <span class="cc_post_tags"> <span class="cc_post_tags_subhead">Tags</span> <a href='https://www.thermofisher.com/blog/clinical-conversations/tag/covid-19/'>COVID-19</a>, <a href='https://www.thermofisher.com/blog/clinical-conversations/tag/mutations/'>Mutations</a>, <a href='https://www.thermofisher.com/blog/clinical-conversations/tag/pcr/'>PCR</a>, <a href='https://www.thermofisher.com/blog/clinical-conversations/tag/sars-cov-2/'>Sars-cov-2</a> </span>   </span> <script type="text/javascript"> cc_toggle('cc_metadata'); cc_toggle('cc_jetpack_shares'); </script> </header><div class="entry-content"><p>The <a href="https://www.thermofisher.com/us/en/home/clinical/clinical-genomics/pathogen-detection-solutions/real-time-pcr-research-solutions-sars-cov-2/mutation-panel.html?cid=fl-mutationpanel">Applied Biosystems TaqMan SARS-CoV-2 Mutation Panel</a> is a scalable solution for your SARS-CoV-2 mutation-screening needs. This panel of assays lets you run a variable number of samples, from just a few to dozens and hundreds, to monitor for one or many SARS-CoV-2 mutations, and it can do this on your current real-time PCR instrument. The secret is, our process combines gold-standard Applied Biosystems TaqMan SNP genotyping technology with a one-step real-time PCR reaction to detect mutations in a sample quickly and efficiently, directly from the RNA. But how does it work?</p> <p><iframe title="How it Works: TaqMan SARS-CoV-2 Mutation Panel" width="760" height="428" src="https://www.youtube.com/embed/n8yRChcJVMs?feature=oembed" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> <p>Starting with RNA isolated from SARS-CoV-2-positive samples, reverse transcription turns that RNA into much more stable cDNA. This is necessary for the rest of the reactions to proceed without damaging the sample, and our system combines this step into the rest of the reactions instead of requiring that it be done separately.</p> <p>Once the cDNA is ready, PCR amplification begins. Our master mix contains a DNA polymerase formulated for high-temperature applications like PCR, forward and reverse primers, a FAM-labeled TaqMan probe for the mutant sequence of interest, and a VIC-labeled TaqMan probe for the corresponding wild-type sequence. The thermal cycler denatures the cDNA templates, separating their strands so that the primers can anneal to their target sequences in the cDNA and the probes can anneal between them. During polymerization, DNA polymerase turns these primers and probes into whole complementary DNA strands, doubling the amount of DNA in the reaction and, importantly, separating the probes from their built-in quenchers. Once the quenchers are separated, the probes’ fluorescence increases, and it is this difference that the real-time PCR system collects as data. Our use of probes optimized for their target sequences removes the hazard of off-target activation and makes sure that the reading clearly indicates mutant or wild-type presence in the sample, as appropriate.</p> <p>After data collection, you can analyze your data with Applied Biosystems Design and Analysis Software 2.5 or later, using the Genotyping Analysis Module. The results are shown as scatter plots, with samples that contain the mutant allele clustered away from those that are wild-type. Patterns in which samples cluster with each other will illuminate your research question and increase knowledge of SARS-CoV-2’s mutation patterns.</p> <p>To learn more about the TaqMan SARS-CoV-2 Mutation Panel, visit <a href="http://www.thermofisher.com/mutationpanel">thermofisher.com/mutationpanel</a>.</p> <p>For Research Use Only. Not for use in diagnostic procedures. 2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license.</p>  </div></article></main></div></div><footer class="site-footer"><div class="wrap"><div class="creds"><p class="footer-links"><a href="https://corporate.thermofisher.com/en/footer/privacy-statement.html" target="_blank" rel="noopener noreferrer">Privacy Statement</a><a href="https://corporate.thermofisher.com/en/footer/terms-and-conditions.html" target="_blank" rel="noopener noreferrer">Terms & Conditions</a><a href="/blog/locations/">Locations</a><a href="/blog/sitemap/">Sitemap</a></p><p>© 2024 <a href="https://www.thermofisher.com">Thermo Fisher Scientific</a>. 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