GENEWIZ from Azenta | Confirmatory Sequencing & Cell Bank Characterization

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Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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project from assay design to data analysis. Simply submit your samples and let us do the rest! </p> <p><span style="background-color: #f0f0f0; color: #000000;">Our experienced scientists will design, develop, and optimize your assays to verify the identity of your sequences and provide you with the final data. We can even utilize samples submitted from existing Azenta Next Generation Sequencing projects.</span><br /> <br /> Azenta’s Sanger-based confirmatory sequencing services can be customized to detect your endogenous or recombinant transcripts/genes, support cell line development, monoclonal antibody heavy chain/light chain sequence confirmation, and transfection verification.<br /> <br /> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/DynamicForm/CreateView?lobId=10&serviceId=1020" target="_blank"><button>Request Quote</button></a>  </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en-GB/Public/Services/Molecular-Genetics/PCR-Solutions" target="_blank"><img alt="" src="/-/media/17001DqPCRdPCRWebBanRight2.ashx?la=en&hash=667C0B7B2E5796BDD1BF6575F2DB2C22E6168950" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner1 { width: 1170px; background-image: url('/-/media/10013-D_Customer-Referral_WebBanFull-Med_Background2.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner1 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner1-left { width: 60%; padding: 5px; } #banner1-right { width: 35%; padding: 15px; } #banner1-left h1 { text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-top: 15px; margin-bottom: 20px; color: #000000; } #banner1-left h3 { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 20px; color: #000000; } #banner1-left p { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-top: 20px; color: #000000; } #banner1-left button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 3px; transition: all .2s ease-in-out; font-size: 15px; font-weight: bold; } #banner1-left button:hover { cursor: pointer; transform: scale(1.1); } #banner1-left button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner1 { width: 95%; height: auto; background: #78d2aa; display: inline-block; } #banner1-left { text-align: center; width: 100%; } #banner1-right { text-align: center; width: 100%; } #banner1-left h1 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #000000; } #banner1-left h3 { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 15px; color: #000000; } #banner1-left img { width: auto; height: auto; } #banner1-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner1-left img { width: 60%; } #banner1-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner1"> <div id="banner1-left"> <h1>Get Rewarded for Your Referrals</h1> <h3>Unlock 20% off your next genomics order*</h3> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02l4rq40" target="_blank"><button>CLAIM YOUR REWARD</button></a> <p>*Terms & conditions apply</p> </div> <div id="banner1-right"> <div id="banner1-right h1"> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; padding: 0; background: #f1f0f0; } .data-container { width; 95%; margin: 0 auto; padding-top: 30px; background: #f1f0f0; } .data { width: 100%; margin: 0 auto; text-align: center; width: 1170px; } .data-step { display: inline-block; vertical-align: top; width: 16%; padding: 5px; margin-bottom: 20px; } .data-step img { width: 150px; margin: 0 auto; margin-bottom: 20px; } .data-step h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .data-icon { text-align: center; height:20%; } @media only screen and (max-width: 1170px) { .data { text-align: center; margin: 0 auto; width: 100%; } .data-step { width: 16%; } } @media only screen and (max-width: 1000px) { .data-step { width: 100%; } } </style> <div class="data-container"> <h2 style="text-align: center; margin-bottom: 30px;">Confirmatory Sequencing Workflow</h2> <div class="data"> <div class="data-step"> <div class="data-icon"> <img alt="" src="/-/media/Images/SubmitSample_Icon.ashx?la=en&hash=5DABA64617438DE4135DC40481532C95267D2241" /> </div> <h3><strong>1. Submit Samples</strong></h3> </div> <div class="data-step"> <div class="data-icon"> <img alt="" src="/-/media/Images/1_PrimerDesign_Icon.ashx?la=en&hash=ECEBA62856E953A668FA6EC9411EF6B7905CF70C" /> </div> <h3><strong>2. Primer Design + Assay Development </strong></h3> </div> <div class="data-step"> <div class="data-icon"> <img alt="" src="/-/media/Images/2_Extraction-Icon.ashx?la=en&hash=30DA1A1F86DADA0ECB170A95C08DCDED6BF194E4" /> </div> <h3><strong>3. Extraction</strong></h3> </div> <div class="data-step"> <div class="data-icon"> <img alt="" src="/-/media/Images/3_PCR_Amp_Icon.ashx?la=en&hash=7830DA6E1D437C71F6F81DB4ED8F8686CB08B615" /> </div> <h3><strong>4. PCR Amplification</strong></h3> </div> <div class="data-step"> <div class="data-icon"> <img alt="" src="/-/media/Images/4_Sanger_Icon.ashx?la=en&hash=B463F19560CE7D127B79F05B27AB6E08F4DFD174" /> </div> <h3><strong>5. Sanger Sequencing</strong></h3> </div> <div class="data-step"> <div class="data-icon"> <img alt="" src="/-/media/Images/5_DataAnalysis_Icon.ashx?la=en&hash=411D89DFFD8AE90F098567AB94D675A692327327" /> </div> <h3><strong>6. Data Analysis</strong></h3> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> h2 { border-bottom: none; margin: 0; padding: 0; } * { box-sizing: border-box; } .column { float: left; width: 50%; padding: 10px; text-align: left; } .row:after { content: ""; display: table; clear: both; } </style> <div class="container"> <h2>Deliverables</h2> <p><strong>Submit your samples and Azenta will take care of the rest!</strong></p> <p>Our experienced scientists design, develop, and optimize assays to verify the identity of your sequences and provide you with: </p> <p> </p> <div class="row"> <div class="column"> <li>Chromatogram file (.ab1)</li> <li>Analysis report, including identification of mutations or discrepancies</li> <li>Sequence file (.seq)</li> </div> <div class="column"> <li>Consensus sequence of results (.seq)</li> <li>Analysis report, including identification of mutations or discrepancies</li> </div> </div> </div> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .wgs-service-options { width: 1170px; margin: 0 auto; text-align: center; margin-top: -150px; padding-top: 190px } .service-container { width: 33%; text-align: center; display: inline-block; vertical-align: top; } .wgs-service { width: 95%; text-align:center; padding: 20px 0 35px 0; margin: 4px; position: relative; } .wgs-service img { height: 110px; } .wgs-service h3 { margin: 0; } .wgs-service p { width: 90%; margin: 0 auto; margin-bottom: 20px; line-height: 20px; } .wgs-service a { color: #3a3a3a; } #wgs-service-1 { background: #f0f0f0; } #wgs-service-1 span { display: none; } #wgs-service-2 { background: #f0f0f0; } #wgs-service-3 { background: #f0f0f0; } #wgs-service-4 { background: #f0f0f0; } #wgs-service-5 { background: #f0f0f0 ; } #wgs-service-6 { background: #f0f0f0 ; } .wgs-service-btn { background: #826ec3; border: 2px solid #826ec3; color: #fff; padding: 10px 20px; -webkit-transition: background-color 0.2s ease-in-out; -moz-transition: background-color 0.2s ease-in-out; -o-transition: background-color 0.2s ease-in-out; transition: background-color 0.2s ease-in-out; } .wgs-service-btn:hover { background: #6959a1; border: 2px solid #6959a1; color: #fff; padding: 10px 20px; } .wgs-service-btn2 { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; } @media only screen and (max-width: 1170px) { .wgs-service-options { width: auto; } .service-container { width: 95%; } .wgs-service { width: 100%; } } </style> <div class="wgs-service-options" id="wholegenome-service-options"> <h2>Applications of Confirmatory Sequencing</h2> <p>Sanger-based confirmatory sequencing of both DNA and RNA/cDNA templates has been utilized to support the following applications:</p> <div class="service-container"> <div class="wgs-service" id="wgs-service-1"> <h3>CRISPR Confirmation</h3> <br /> <p>Confirmation of target CRISPR integrations (& identification of off-target effects)<span style="display: inline !important;"></span></p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package" target="_blank"><button class="wgs-service-btn">Learn More</button></a> </div> </div> <div class="service-container"> <div class="wgs-service" id="wgs-service-2"> <h3>Next Generation Sequencing Confirmation</h3> <br /> <p>Confirmation of mutations identified by NGS screens</p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/confirm-your-ngs-data" target="_blank"><button class="wgs-service-btn">Learn More</button></a> </div> </div> <div class="service-container"> <div class="wgs-service" id="wgs-service-3"> <h3>Antibody Sequencing Confirmation</h3> <br /> <p>Confirmation of heavy-chain/light-chain sequences for monoclonal antibodies</p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/confirm-your-antibody-sequences" target="_blank"><button class="wgs-service-btn">Learn More</button></a> </div> </div> <div class="service-container"> <div class="wgs-service" id="wgs-service-4"> <h3>Cell Line/Cell Bank Confirmation</h3> <br /> <p>Genotyping & confirmation of engineered cell lines (cell line ID/stability)</p> </div> </div> <div class="service-container"> <div class="wgs-service" id="wgs-service-5"> <h3>Gene Synthesis Product Confirmation</h3> <br /> <p>Confirmation of clones and gene editing products    </p> </div> </div> <div class="service-container"> <div class="wgs-service" id="wgs-service-6"> <h3>Transfection Verification</h3> <br /> <p>Confirmation of genomic integrations in cell lines & viral vectors (lentivirus or AAV)</p> </div> </div> <br /> <br /> <div class="rna-seq-services" id="rna-seq-services">Find out more about our plant gene<span style="text-align: left;">tics services OR inqu</span><span style="text-align: left;">ire about unique sample type:</span><br /> <p> </p> <p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/confirm-seq-inquiry" target="_blank"><button class="wgs-service-btn2">Submit Inquiry</button></a>  </p> </div> </div> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; 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border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h3>Ensure Complete Plasmid Validation on Your First Attempt</h3> <h4>with Oxford Nanopore Sequencing</h4> <a href="https://hubs.ly/Q02BXsZq0" target="_blank"> <br /> <button> READ BLOG</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <p><strong>Complete Solution: </strong>Nucleic Acid extraction to Sanger sequencing & data analysis<strong><br /> </strong></p> <div> </div> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <p><strong>Complete Sequence Coverage: </strong>Our team ensures complete sequencing of your region of interest, including high GC content regions, and TA Cloning, as needed</p> </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <p><strong><span>Superior Support: </span></strong><span>Dedicated Azenta scientist, expert technical support, and consistent, transparent communication with weekly project updates</span><strong><span><br /> </span></strong></p> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <p><strong><span>Sample flexibility: </span></strong><span>Our team has experience working with many sample types including: cell pellets, tissue samples, gDNA, RNA/cDNA, plasmids, FFPE samples and more.</span><strong><span><br> </span></strong></p> </div> </div> </div> </div> </div> </div><script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-60 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h2>Technical Resources</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <a href='https://www.genewiz.com/en/Public/Resources/Sample-Submission-Guidelines/Molecular-Genetics-Sample-Submission-Guidelines' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Prepare_Samples_Web-Icon-Workflow_Purple.ashx?h=91&la=en&w=91&hash=46CD2A5C3BAF8BA51A6B26A29368BBB9171BDA5C" alt="Building" width="91" height="91" /> </div> <div class="row"> <h3>Sample Type and Submission Guidelines</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='https://www.genewiz.com/en/Public/Resources/FAQs/FAQs-Molecular-Genetics' target='blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/chat-bubble_Purple.ashx?h=110&la=en&w=210&hash=20D4E093CF40A1D72E7CE3721F0FDEAC239B298C" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Frequently Asked Questions</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='https://www.genewiz.com/en/Public/Resources/Citations-Publications#molgen-citations' target='blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/open-book_Purple.ashx?h=110&la=en&w=210&hash=7574E80B1788AA80307BB72434FB52FD4A46912A" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Azenta Citations</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Related Services</h2> <p></p> </div> <ul class="list-unstyled row row row-centered"> <li class='col-sm-3'> <a href='https://web.genewiz.com/aav-plasmid-synthesis' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/cDNA-verification-services_375x561_Purple.ashx?h=110&la=en&w=164&hash=2F52AA31CFA8567D566B907E2379AA0F21DA2EE0" alt="" width="164" height="110" /> </div> <div class="row"> <h3>GLP Confirmatory Sequencing</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='/Public/Services/Sanger-Sequencing/Premium-Services' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/premium-services-110x210_Purple.ashx?h=110&la=en&w=210&hash=EE17256D3CB28B1FC2B31A465EABD63B3EE9375D" alt="" width="210" height="110" /> </div> <div class="row"> <h3>Primer Walking</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='https://web.genewiz.com/aav-plasmid-prep' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/PCR-PLUS-SEQUENCING-CRISPR-ANALYSIS-icon_210x110_Purple_DESIGN.ashx?h=111&la=en&w=211&hash=2868F8C7DD7CE76FE77AAA2101B456D7878AE4AD" alt="Building" width="211" height="111" /> </div> <div class="row"> <h3>PCR Plus Sequencing CRISPR Analysis</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Mutation-Analysis_110x210_Purple.ashx?h=110&la=en&w=210&hash=2806094D29EF73CDD936C544102848CE25D474B3" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Mutation Analysis</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>How to Order</h2> </div> <div class="row"> *Samples must arrive at the Azenta New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. 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