Mouse IL-2 ELISA - Quantikine M2000: R&D Systems

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class="ds_title">Mouse IL-2 ELISA Kit - Quantikine </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: M2000</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 57 Citations </a> </span> <span class="reviews review_stars_4"> <a href="#product-reviews" id="product_reviews" class="jump_to" data-tab="6" data-tabname="product-reviews"> 7 Reviews </a> </span> <a href="/reviews/M2000" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/mouse-il-2-quantikine-elisa-kit_m2000" method="post" 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Up to 30% off + FREE shipping. <a href="/promotions#reagents25" target="_blank">See Details</a>.</p> </div> <div class="ds_atc_pre_buttons"></div> <div class="ds_atc_buttons"> <a href="/quote/instruments/ella-automated-immunoassay-system" class="btn btn-secondary atc_request_quote_simple_plex_assay" style="display:none" rel="nofollow">Request a Quote</a> <a href="/services/bulk-quote-request?catnum=M2000&product=Mouse IL-2 Quantikine ELISA Kit" class="btn btn-secondary atc_hidden bulk_btn" style="display:none;" rel="nofollow" title="Competitive discounts on products purchased in bulk quantities">Bulk Orders</a> <button class="btn btn-primary atc_hidden atc_btn form-submit icon-before" style="display:none;" title="Enter quantities above and click to add to cart" type="submit" id="edit-add-to-cart" name="add_to_cart_btn" value="Add to Cart"><span class="icon glyphicon glyphicon-plus" aria-hidden="true"></span> Add to Cart</button> </div> <div class="ds_atc_post_buttons"></div> </div> <input type="hidden" name="form_build_id" value="form-DAAw5X8R7zElPxl5yJ83OXxGwjBuLrbttrtZnFEDh20" /> <input type="hidden" name="form_id" value="atc_datasheet_form" /> </div></form> </div> </div> <div class="product_image_wrapper col-xs-12 col-md-4 col-md-pull-8"> <div class="product_image"> <span class="product_image_label"> </span> <a class="ds-image overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="Mouse IL-2 ELISA Standard Curve" data-footer="Mouse IL-2 ELISA Standard Curve" title="Cat. No. M2000 - Mouse IL-2 Quantikine ELISA Kit" href="https://resources.rndsystems.com/images/datasheets/elisa/DATA_IL2_M2000_ELISA_926.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/elisa/DATA_IL2_M2000_ELISA_926.jpg" class="img-fluid" alt="Mouse IL-2 ELISA Standard Curve" title="Mouse IL-2 ELISA Standard Curve"> </a> <div class="search_image_other_count"> <span class="search_more_images">2 Images</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="Mouse IL-2 ELISA Linearity" data-title="Mouse IL-2 ELISA Linearity" title="Cat. No. M2000 - Mouse IL-2 Quantikine ELISA Kit" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/elisa/IL-2_M2000_ELISA_229.jpg"> <img src="https://resources.rndsystems.com/images/datasheets/elisa/IL-2_M2000_ELISA_229.jpg" class="img-fluid" loading="lazy" alt="Mouse IL-2 ELISA Linearity" title="Mouse IL-2 ELISA Linearity"> </a> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/il-2?category=ELISAs" class="btn btn-secondary">All IL-2 ELISAs</a> </div> </div> <div class="tabbed_content" id="rnd_elisa"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_2" data-tab="2" data-tabname="assay-procedure" id="dsTabs_assay-procedure"> <span>Procedure</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (57)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews (7) </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_precision" id="product-anchor-ds_precision">Precision</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_recovery" id="product-anchor-ds_recovery">Recovery</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_linearity" id="product-anchor-ds_linearity">Linearity</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <div id="ds_summary" class="datasheet_section"> <h2>Mouse IL-2 Quantikine ELISA Kit Summary</h2> <div class="ds_table summary_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Type</div> <div class="ds_table_cell summary_table_data">Solid Phase Sandwich ELISA</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Format</div> <div class="ds_table_cell summary_table_data">96-well strip plate</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Length</div> <div class="ds_table_cell summary_table_data">4.5 hours</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sample Type & Volume Required Per Well</div> <div class="ds_table_cell summary_table_data">Cell Culture Supernates (50 uL), Serum (50 uL)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sensitivity</div> <div class="ds_table_cell summary_table_data">3 pg/mL</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Range</div> <div class="ds_table_cell summary_table_data">15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data">Natural and recombinant mouse IL-2</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Cross-reactivity</div> <div class="ds_table_cell summary_table_data">< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Interference</div> <div class="ds_table_cell summary_table_data">No significant interference observed with available related molecules.</div> </div> </div> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="M2000"></div> <div class="datasheet_section"> <h2>Product Summary</h2> <div> The Quantikine Mouse IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-2 <span>levels in cell culture supernates and serum. It contains <EM>E. coli</EM>-expressed recombinant mouse IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-2 accurately. Results obtained using natural mouse IL-2 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-2.</span> </div> </div> <div id="ds_precision" class="datasheet_section"> <h2>Precision</h2> <div><strong>Intra-Assay Precision (Precision within an assay)</strong> </div><div><strong>Inter-Assay Precision (Precision between assays)</strong> </div> <p class="precision_title"><strong>Cell Culture Supernates, Serum</strong></p> <table class="ds_table_html precision_table"> <thead> <tr> <th class="p1"></th> <th colspan="3">Intra-Assay Precision</th> <th colspan="3">Inter-Assay Precision</th> </tr> </thead> <tbody> <tr> <td><strong>Sample</strong></td> <td>1</td> <td>2</td> <td>3</td> <td>1</td> <td>2</td> <td>3</td> </tr> <tr> <td><strong>n</strong></td> <td>20</td> <td>20</td> <td>20</td> <td>20</td> <td>20</td> <td>20</td> </tr> <tr> <td><strong>Mean (pg/mL)</strong></td> <td>70.4</td> <td>187</td> <td>595</td> <td>70.9</td> <td>175</td> <td>559</td> </tr> <tr> <td><strong>Standard Deviation</strong></td> <td>3.9</td> <td>10</td> <td>23.4</td> <td>3.6</td> <td>9.6</td> <td>24.5</td> </tr> <tr> <td><strong>CV%</strong></td> <td>5.5</td> <td>5.3</td> <td>3.9</td> <td>5.1</td> <td>5.5</td> <td>4.4</td> </tr> </tbody> </table><br/> </div> <div id="ds_recovery" class="datasheet_section"> <h2>Recovery</h2> <div> <p>The recovery of mouse IL-2 spiked to three levels throughout the range of the assay was evaluated.<p> </div> <table class="ds_table_html recovery_table"> <thead> <th>Sample Type</th> <th>Average % Recovery</th> <th>Range %</th> </thead> <tr> <td>Cell Culture Supernates (n=6)</td> <td>102</td> <td>84-110</td> </tr> <tr> <td>Serum (n=6)</td> <td>102</td> <td>96-108</td> </tr> </table> </div> <div id="ds_linearity" class="datasheet_section"> <h2>Linearity</h2> <div>To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-2 in each matrix were diluted with Calibrator Diluent and then assayed.</div> <div> <img class=" hideoverflow quantikineLinearityImage" alt="Mouse IL-2 ELISA Linearity" src="https://resources.rndsystems.com/images/datasheets/elisa/IL-2_M2000_ELISA_229.jpg"> </div> <div class="marginBottom"></div> </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> N/A </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="Mouse IL-2 ELISA Standard Curve" data-footer="Mouse IL-2 ELISA Standard Curve" title="Cat. No. - Mouse IL-2 ELISA Standard Curve" href="https://resources.rndsystems.com/images/datasheets/elisa/DATA_IL2_M2000_ELISA_926.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/elisa/DATA_IL2_M2000_ELISA_926.jpg" class="img-fluid" alt="Mouse IL-2 ELISA Standard Curve" title="Mouse IL-2 ELISA Standard Curve"> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Mouse IL-2 ELISA Standard Curve</span> </p> </div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/m2000.pdf?v=20250218" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/mouse-il-2-quantikine-elisa-kit_m2000">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//resources.rndsystems.com/pdfs/msds/m2000.pdf?v=20250218">SDS</a> </div> </div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Storage</div> <div class="ds_table_cell prep_table_data">Store the unopened product at 2 - 8 °C. Do not use past expiration date. </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: IL-2</h2> <p>IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.</p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name:</div> <div class="ds_table_cell background_table_data">Interleukin 2</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs:</div> <div class="ds_table_cell background_table_data">3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names:</div> <div class="ds_table_cell background_table_data">Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF</div> </div> </div> </div> </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/cancer-biomarkers">Cancer Biomarkers</a> </li> <li> <a href="/research-area/common-gamma-chain-receptor-family">Common gamma Chain Receptor Family</a> </li> <li> <a href="/research-area/conventional-classical-dendritic-cells">Conventional/Classical Dendritic Cells</a> </li> <li> <a href="/research-area/cytokines-and-receptors-in-allergy-and-asthma">Cytokines and Receptors in Allergy and Asthma</a> </li> <li> <a href="/research-area/cytokines-and-receptors-in-tfh-cell-differentiation-and-function">Cytokines and Receptors in Tfh Cell Differentiation and Function</a> </li> <li> <a href="/research-area/gamma-delta-t-cells">gamma delta T Cells</a> </li> <li> <a href="/research-area/group-1-ilcs">Group 1 ILCs</a> </li> <li> <a href="/research-area/infectious-diseases">Infectious Diseases</a> </li> <li> <a href="/research-area/inflammatory-mediators">Inflammatory Mediators</a> </li> <li> <a href="/research-area/isolation--culture--and-identification-of-natural-killer-cells">Isolation, Culture, and Identification of Natural Killer Cells</a> </li> <li> <a href="/research-area/multiple-sclerosis-and-myelin-related-molecules">Multiple Sclerosis and Myelin Related Molecules</a> </li> <li> <a href="/research-area/ncr--ilc3">NCR+ILC3</a> </li> <li> <a href="/research-area/neuroinflammatory-factors">Neuroinflammatory Factors</a> </li> <li> <a href="/research-area/preeclampsia">Preeclampsia</a> </li> <li> <a href="/research-area/regulatory-t-cells--tregs">Regulatory T Cells (Tregs)</a> </li> <li> <a href="/research-area/search-cytokine-and-growth-factor-products">Search Cytokine and Growth Factor Products</a> </li> <li> <a href="/research-area/t-cell-cytokine-signaling">T Cell Cytokine Signaling</a> </li> <li> <a href="/research-area/th1-cells">Th1 Cells</a> </li> <li> <a href="/research-area/th2-cells">Th2 Cells</a> </li> <li> <a href="/research-area/th9-cells">Th9 Cells</a> </li> </ul> </div> <div class="prop_65_warning"><div class="prop_65_warning">&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.</div></div> </div> <div class="tab_content tab_2" data-tab="2" data-tabname="product-assay-procedure" id="product-assay-procedure"> <a id="assay-procedure"></a> <div id="boxes">  <h2>Assay Procedure</h2> <strong>Refer to the product <datasheet> for complete assay procedure.</strong><br /><br /> <div class="psp_procedure_header"> Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate. </div> <ol class="psp_procedure"> <li><span>  Prepare all reagents, standard dilutions, and samples as directed in the product insert.</span></li> <li><span>  Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.</span></li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong>50 µL Assay Diluent</strong><br /><img src="//resources.rndsystems.com/images/site/wells-plain.png" align="right" /></div> <li><span>  Add 50 µL of Assay Diluent to each well.</span> </li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong class="AssayStepMultiLine">50 µL Standard, Control, or Sample</strong><br /><img src="//resources.rndsystems.com/images/site/wells-plain.png" align="right" /></div> <li><span>  Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.</span> </li> <li><span>  Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.</span></li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong>100 µL Conjugate</strong><br /><img src="//resources.rndsystems.com/images/site/wells-plain.png" align="right" /></div> <li><span>  Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.</span></li> <li><span>  Aspirate and wash 5 times.</span></li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong>100 µL Substrate Solution</strong><br /><img src="//resources.rndsystems.com/images/site/wells-blue.png" align="right" /></div> <li><span>  Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.</span></li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong>100 µL Stop Solution</strong><br /><img src="//resources.rndsystems.com/images/site/wells-yellow.png" align="right" /></div> <li><span>  Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.</span></li> </ol> </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for Mouse IL-2 Quantikine ELISA Kit</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">57</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">10</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Mouse"> Mouse </option> <option class="citation_filterRadio" value="species" data-filter="Transgenic Mouse"> Transgenic Mouse </option> </select> </div> <input type="hidden" id="selAntibody" value="All"> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="BALF, Serum"> BALF, Serum </option> <option class="citation_filterRadio" value="sampletype" data-filter="Cell Culture Supernates"> Cell Culture Supernates </option> <option class="citation_filterRadio" value="sampletype" data-filter="Plasma"> Plasma </option> <option class="citation_filterRadio" value="sampletype" data-filter="Serum"> Serum </option> <option class="citation_filterRadio" value="sampletype" data-filter="Tissue Homogenates"> Tissue Homogenates </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="57" data-max-show="10"> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/39261466"> IL-2 delivery to CD8+ T cells during infection requires MRTF/SRF-dependent gene expression and cytoskeletal dynamics </a> </span><br/> Authors: Maurice, D;Costello, P;Diring, J;Gualdrini, F;Frederico, B;Treisman, R;<br/> Nature communications<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/38842347"> CLN-617 retains IL-2 and IL-12 in injected tumors to drive robust and systemic immune-mediated antitumor activity </a> </span><br/> Authors: Mehta, NK;Rakhra, K;Meetze, KA;Li, B;Momin, N;Chang, JY;Wittrup, KD;Baeuerle, PA;Michaelson, JS;<br/> Cancer immunology research<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse, Transgenic Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/38806671"> Mycobacterium tuberculosis produces D-serine under hypoxia to limit CD8+ T cell-dependent immunity in mice </a> </span><br/> Authors: Cheng, H;Ji, Z;Wang, Y;Li, S;Tang, T;Wang, F;Peng, C;Wu, X;Cheng, Y;Liu, Z;Ma, M;Wang, J;Huang, X;Wang, L;Qin, L;Liu, H;Chen, J;Zheng, R;Feng, CG;Cai, X;Qu, D;Ye, L;Yang, H;Ge, B;<br/> Nature microbiology<br/> Species: Mouse, Transgenic Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/37873241"> IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHC CII complexes </a> </span><br/> Authors: Qiu, Z;Khalife, J;Lin, AP;Ethiraj, P;Jaafar, C;Chiou, L;Huelgas-Morales, G;Aslam, S;Arya, S;Gupta, YK;Dahia, PLM;Aguiar, RCT;<br/> bioRxiv : the preprint server for biology<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/37764156"> Immune-Stimulating Potential of Lacticaseibacillus rhamnosus LM1019 in RAW 264.7 Cells and Immunosuppressed Mice Induced by Cyclophosphamide </a> </span><br/> Authors: You, Y;Kim, SH;Kim, CH;Kim, IH;Shin, Y;Kim, TR;Sohn, M;Park, J;<br/> Microorganisms<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="BALF, Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/37667317"> STING signaling activation modulates macrophage polarization via CCL2 in radiation-induced lung injury </a> </span><br/> Authors: Ni, J;Guo, T;Zhou, Y;Jiang, S;Zhang, L;Zhu, Z;<br/> Journal of translational medicine<br/> Species: Mouse<br/> Sample Types: BALF, Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/36399563"> CLEC-1 is a death sensor that limits antigen cross-presentation by dendritic cells and represents a target for cancer immunotherapy </a> </span><br/> Authors: M Drouin, J Saenz, V Gauttier, B Evrard, G Teppaz, S Pengam, C Mary, A Desselle, V Thepenier, E Wilhelm, E Merieau, C Ligeron, I Girault, MD Lopez, C Fourgeux, D Sinha, I Baccelli, A Moreau, C Louvet, R Josien, J Poschmann, N Poirier, E Chiffoleau<br/> Science Advances, 2022-11-18;8(46):eabo7621.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/35716476"> Immunomodulatory effects of cyclotides isolated from Viola odorata in an experimental autoimmune encephalomyelitis animal model of multiple sclerosis </a> </span><br/> Authors: L Dayani, MS Dinani, M Aliomrani, H Hashempour, J Varshosaz, A Taheri<br/> Oncogene, 2022-06-10;64(0):103958.<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/35077397"> Commensal oral microbiota induces osteoimmunomodulatory effects separate from systemic microbiome in mice </a> </span><br/> Authors: JD Hathaway-S, JD Aartun, NA Poulides, MB Kuhn, BE McCormick, ME Chew, E Huang, RP Darveau, C Westwater, CM Novince<br/> JCI Insight, 2022-02-22;0(0):.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/33849389"> Augmenting ATG14 alleviates atherosclerosis and inhibits inflammation via promotion of autophagosome-lysosome fusion in macrophages </a> </span><br/> Authors: H Zhang, S Ge, B Ni, K He, P Zhu, X Wu, Y Shao<br/> Autophagy, 2021-05-04;0(0):.<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <div id="ds_extra_cits"> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/33654081"> Structures of mouse and human GITR-GITRL complexes reveal unique TNF superfamily interactions </a> </span><br/> Authors: 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R Li, S Wu, Z Abliz, C Li, XL Li, XL Ma, LL Wang, WS Zheng, YX Han, JD Jiang<br/> Nat Commun, 2019-04-30;10(1):1981.<br/> Species: Mouse<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/30608959"> Topical essential fatty acid oil on wounds: Local and systemic effects </a> </span><br/> Authors: BG Lania, J Morari, AR Almeida, MND Silva, G Vieira-Dam, KA Lins, CL César, LA Velloso, NB Maia, ML Cintra, PENF Velho<br/> PLoS ONE, 2019-01-04;14(1):e0210059.<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/29620148"> Carnosic acid protects mice from high-fat diet-induced NAFLD by 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Genet., 2007-02-04;39(3):329-37.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/17202346"> Intercellular adhesion molecule-1 expression is required on multiple cell types for the development of experimental autoimmune encephalomyelitis. </a> </span><br/> Authors: Bullard DC, Hu X, Schoeb TR, Collins RG, Beaudet AL, Barnum SR<br/> J. Immunol., 2007-01-15;178(2):851-7.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/17143984"> Tetrathiomolybdate is effective in a mouse model of arthritis. </a> </span><br/> Authors: McCubbin MD, Hou G, Abrams GD, Dick R, Brewer GJ<br/> J. Rheumatol., 2006-10-15;33(12):2501-6.<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/16891459"> Blockade of transforming growth factor-{beta} signaling in tumor-reactive CD8(+) T cells activates the antitumor immune response cycle. </a> </span><br/> Authors: Zhang Q, Yang XJ, Kundu SD, Pins M, Javonovic B, Meyer R, Kim SJ, Greenberg NM, Kuzel T, Meagher R, Guo Y, Lee C<br/> Mol. Cancer Ther., 2006-07-01;5(7):1733-43.<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/16713992"> Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation. </a> </span><br/> Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X<br/> Biochem. Biophys. Res. Commun., 2006-05-12;345(3):1122-30.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/16514061"> PKCtheta and PKA are antagonistic partners in the NF-AT transactivation pathway of primary mouse CD3+ T lymphocytes. </a> </span><br/> Authors: Hermann-Kleiter N, Thuille N, Pfeifhofer C, Gruber T, Schafer M, Zitt C, Hatzelmann A, Schudt C, Leitges M, Baier G<br/> Blood, 2006-03-02;107(12):4841-8.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/15325513"> Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes. </a> </span><br/> Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF<br/> Comp. Immunol. Microbiol. Infect. Dis., 2004-11-01;27(6):393-411.<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/15502157"> EphrinB1 is essential in T-cell-T-cell co-operation during T-cell activation. </a> </span><br/> Authors: Yu G, Luo H, Wu Y, Wu J<br/> J. Biol. Chem., 2004-10-22;279(53):55531-9.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/15240805"> Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration. </a> </span><br/> Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M<br/> J. Neurosci., 2004-07-07;24(27):6133-43.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/12139394"> Anti-tumor mechanism of Z-100, an immunomodulatory Arabinomannan extracted from Mycobacterium tuberculosis strain Aoyama B, on pulmonary metastases of B16F10 melanoma: restoration of helper T cell responses via suppression of glucocorticoid-genesis. </a> </span><br/> Authors: Oka H, Emori Y, Sasaki H, Shiraishi Y, Yoshinaga K, Kurimoto T<br/> Microbiol. Immunol., 2002-01-01;46(5):343-51.<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/11449364"> The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls. </a> </span><br/> Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR<br/> Eur. J. Immunol., 2001-07-01;31(7):2104-15.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> </div> </ol> </div> <div class="clearfix"><button class="btn btn-quinary ds_extra_cits pull-right toggle_cits_down" id="toggle_cits"></button></div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <ol id="faq_list"> <li> <span class="faq_question"><p>Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?</p></span> <ul class="faq_answer"> <li><p>A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.</p></li> </ul> </li> </ol> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/elisas"> View all ELISA FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="m2000" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Mouse IL-2 Quantikine ELISA Kit</h3> <div class="row"> <div class="col-xs-12"> <div class="datasheet_section"> <div class="row"> <div class="col-md-6"> <p> Average Rating: 4.4 <span class="review_stars_4"></span> (Based on 7 Reviews) </p> <div class="review_rating_progress"> <div class="row"> <div class="col-xs-3"> 5 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="42.857142857143" aria-valuemin="0" aria-valuemax="100" style="width:42.857142857143%"> </div> </div> </div> <div class="col-xs-3"> 42.86% </div> </div> <div class="row"> <div class="col-xs-3"> 4 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="57.142857142857" aria-valuemin="0" aria-valuemax="100" style="width:57.142857142857%"> </div> </div> </div> <div class="col-xs-3"> 57.14% </div> </div> <div class="row"> <div class="col-xs-3"> 3 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="0" aria-valuemin="0" aria-valuemax="100" style="width:0%"> </div> </div> </div> <div class="col-xs-3"> 0% </div> </div> <div class="row"> <div class="col-xs-3"> 2 Star </div> <div class="col-xs-6"> <div class="progress"> <div 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<div class="col-md-12"> <div class="reviews_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper"> <select class="review_filter_rating"> <option value="all">All Ratings</option> <option value="5">5</option> <option value="4">4</option> </select> </div> <div class="select_wrapper"> <select class="review_filter_application"> <option value="all">All Applications</option> </select> </div> </div> </div> </div> <br/> <div class="review_row" data-rating="5" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_5">Mouse IL-2 Quantikine ELISA Kit</div> <div class="review_date">By <span class="review_reviewer">Anonymous</span> on 12/12/2022 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Cell culture supernatant </span> </div> </div> <div class="review_how_used"><p>Virus-infected Cell culture supernatant diluted 1:10,000</p></div> </div> <div class="col-xs-12 col-sm-2"> <a class="ds-image" data-toggle="lightbox" href="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_m2000_76616.png" data-title="Mouse IL-2 Quantikine ELISA Kit M2000" data-gallery="Review-images"> <img loading="lazy" class="img-fluid review_image" alt="Mouse IL-2 Quantikine ELISA Kit M2000" title="Mouse IL-2 Quantikine ELISA Kit M2000" src="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_m2000_76616.png"/> </a> </div> </div> <hr/> </div> <div class="review_row" data-rating="5" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_5">Mouse IL-2 Quantikine ELISA Kit</div> <div class="review_date">By <span class="review_reviewer">Thanh Nguyen</span> on 10/18/2020 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> activated mouse CD8 T cell </span> </div> </div> </div> </div> <hr/> </div> <div class="review_row" data-rating="4" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_4">Mouse IL-2 Quantikine ELISA Kit</div> <div class="review_date">By <span class="review_reviewer">Dhivya Sridaran</span> on 04/04/2019 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Jurkat human acute T cell leukemia cell line,MOLT-4 human acute lymphoblastic leukemia cell line </span> </div> </div> </div> </div> <hr/> </div> <div class="review_row" data-rating="4" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_4">Mouse IL-2 Quantikine ELISA Kit</div> <div class="review_date">By <span class="review_reviewer">Shambhavi Bhagwat</span> on 03/21/2019 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> activated mouse CD8 T cell </span> </div> </div> </div> </div> <hr/> </div> <div class="review_row" data-rating="4" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_4">Mouse IL-2 Quantikine ELISA Kit</div> <div class="review_date">By <span class="review_reviewer">Anonymous</span> on 06/19/2018 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Serum </span> </div> </div> </div> </div> <hr/> </div> <div class="review_row" data-rating="4" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_4">Mouse IL-2 Quantikine ELISA Kit</div> <div class="review_date">By <span class="review_reviewer">Anonymous</span> on 06/12/2018 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Serum </span> </div> </div> </div> </div> <hr/> </div> <div class="review_row" data-rating="5" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_5">Mouse IL-2 Quantikine ELISA Kit</div> <div class="review_date">By <span class="review_reviewer">Hannah White</span> on 05/24/2017 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Concanavalin A-stimulated splenocytes </span> </div> </div> </div> </div> <hr/> </div> </div> </div> </div> <div id="sidebar" class="col-xs-12 col-sm-4 col-md-3 col-lg-3 col-xl-3"> <div class="distributor_region"></div> <div class="widget"> <div class="widget_title">Related Products</div> <div class="widget_content" id="related_products_area"> <ul class="ds_details"> <li> <a href="/target/il-2"> All Products for IL-2 </a> </li> <li> <a 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Mouse IL-2 ELISA - Quantikine M2000: R&D Systems