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First, some background on the use of pathogen reduction in platelet" /> <meta property="og:url" content="https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/" /> <meta property="og:site_name" content="Behind the Bench" /> <meta property="article:publisher" content="https://www.facebook.com/thermofisher" /> <meta property="article:published_time" content="2016-01-11T19:26:34+00:00" /> <meta property="article:modified_time" content="2016-05-12T22:54:20+00:00" /> <meta property="og:image" content="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized.jpg" /> <meta property="og:image:width" content="1050" /> <meta property="og:image:height" content="600" /> <meta property="og:image:type" content="image/jpeg" /> <meta name="author" content="Angie Lackey" /> <meta name="twitter:card" content="summary_large_image" /> <meta name="twitter:creator" content="@thermofisher" /> <meta name="twitter:site" content="@thermofisher" /> <meta name="twitter:label1" content="Written by" /> <meta name="twitter:data1" content="Angie Lackey" /> <meta name="twitter:label2" content="Est. reading time" /> <meta name="twitter:data2" content="1 minute" /> <script type="application/ld+json" class="yoast-schema-graph">{"@context":"https://schema.org","@graph":[{"@type":"WebPage","@id":"https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/","url":"https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/","name":"What Happened to my Platelets? - Behind the Bench","isPartOf":{"@id":"https://www.thermofisher.com/blog/behindthebench/#website"},"primaryImageOfPage":{"@id":"https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/#primaryimage"},"image":{"@id":"https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/#primaryimage"},"thumbnailUrl":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized.jpg","datePublished":"2016-01-11T19:26:34+00:00","dateModified":"2016-05-12T22:54:20+00:00","author":{"@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/aee4ecb3303ff14eb0ffd6f4b0554fab"},"breadcrumb":{"@id":"https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/#primaryimage","url":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized.jpg","contentUrl":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized.jpg","width":1050,"height":600},{"@type":"BreadcrumbList","@id":"https://www.thermofisher.com/blog/behindthebench/what-happened-to-my-platelets/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://www.thermofisher.com/blog/behindthebench/"},{"@type":"ListItem","position":2,"name":"What Happened to my Platelets?"}]},{"@type":"WebSite","@id":"https://www.thermofisher.com/blog/behindthebench/#website","url":"https://www.thermofisher.com/blog/behindthebench/","name":"Behind the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://www.thermofisher.com/blog/behindthebench/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/aee4ecb3303ff14eb0ffd6f4b0554fab","name":"Angie Lackey","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/image/","url":"https://secure.gravatar.com/avatar/b919ead082f9390abedc155b616a0d15?s=96&d=mm&r=g","contentUrl":"https://secure.gravatar.com/avatar/b919ead082f9390abedc155b616a0d15?s=96&d=mm&r=g","caption":"Angie Lackey"},"url":"https://www.thermofisher.com/blog/author/angie-lackey/"}]}</script>  <link rel='dns-prefetch' href='//www.thermofisher.com' /> <link rel='dns-prefetch' href='//use.fontawesome.com' /> <link rel="alternate" type="application/rss+xml" title="Behind the Bench » Feed" href="https://www.thermofisher.com/blog/behindthebench/feed/" /> <link rel="alternate" type="application/rss+xml" title="Behind the Bench » Comments Feed" href="https://www.thermofisher.com/blog/behindthebench/comments/feed/" /> <link rel="alternate" type="application/rss+xml" title="Behind the Bench » What Happened to my Platelets? 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class="content-sidebar-wrap"><main class="content"><article class="post-6661 post type-post status-publish format-standard has-post-thumbnail category-immunology tag-ion-torrent tag-next-generation-sequencing tag-targeted-rna-sequencing tag-whole-transcriptome entry" aria-label="What Happened to my Platelets?"><header class="entry-header"><h1 class="entry-title">What Happened to my Platelets?</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/angie-lackey/" title="Posts by Angie Lackey" rel="author">Angie Lackey</a> </span> <span class="post-info-date"> 01.11.2016 </span> </header><div class="entry-content"><p><img decoding="async" class=" wp-image-7177 alignright" src="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized.jpg" alt="Science up Close BloodCells Card Sized" width="508" height="290" srcset="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized.jpg 1050w, https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized-300x171.jpg 300w, https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized-768x439.jpg 768w, https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized-1024x585.jpg 1024w, https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2016/01/Science-up-Close-BloodCells-Card-Sized-400x229.jpg 400w" sizes="(max-width: 508px) 100vw, 508px" /></p> <p>A research team, comprised of scientists from Sweden, Germany and Canada, have published a paper profiling the changes in gene expression from stored platelets that had been subjected to various pathogen reduction methods using the Ion AmpliSeq™ Transcriptome Solution and the Ion Proton™ System.</p> <p>First, some background on the use of pathogen reduction in platelet preparation. Platelet concentrates are prepared at blood banks and stored for a few days until needed for transfusion. Two things need to occur to make the best use of these concentrates: (1) an increase in the shelf-life of the platelet concentrates samples is desirable and (2) a reduction in the number of transfusion-associated infections is critical.</p> <p>Various methods of pathogen reduction are commonly used and this study by <a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133070" target="_blank">Osman, et al (2015)</a> evaluated the effect of four conditions compared to a control group. Significant changes in the gene expression profiles of the five groups were evaluated, along with miRNA-mRNA correlations. Their findings indicate that “…development of approaches targeting pathogens more specifically, while sparing platelet nucleic acids level and function, would be highly desirable…”.</p> <p> </p> <p>To learn more about their discoveries, you can access the full article <a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133070">here</a>. And find out how <a href="http://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-select-targets/ampliseq-target-selection/ampliseq-transcriptome.html">Ion AmpliSeq Transcriptome Solution</a> can transform your research study by profiling over 20,000 genes in less than 2 days, using as little as 100 pg of high-quality RNA or 10 ng of FFPE RNA.</p> <p><strong>For Research Use Only. 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