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<!DOCTYPE html> <html > <head> <meta charset="utf-8"> <meta rel="search" type="application/opensearchdescription+xml" href="/open_search.xml" title="Academia.edu"> <meta content="width=device-width, initial-scale=1" name="viewport"> <meta name="google-site-verification" content="bKJMBZA7E43xhDOopFZkssMMkBRjvYERV-NaN4R6mrs"> <meta name="csrf-param" content="authenticity_token" /> <meta name="csrf-token" content="uj+RJtJ4wL9kk6pWYiez0h9IaAS5J/XtgVG0W+8iQ7tVrXkwfPrClF3uE/lwz+ys2VDfzBF9HFayXV1G/YdaQA==" /> <meta name="citation_title" content="Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants" /> <meta name="citation_publication_date" content="2020/01/01" /> <meta name="citation_journal_title" content="Vegetos" /> <meta name="citation_author" content="Janardan Lamichhane" /> <meta name="twitter:card" content="summary" /> <meta name="twitter:url" content="https://www.academia.edu/68898159/Phytochemical_profiling_using_HRLCMS_and_evaluation_of_antioxidant_and_antibacterial_activities_of_Nepalese_medicinal_plants" /> <meta name="twitter:title" content="Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants" /> <meta name="twitter:description" content="Medicinal plants have been conspicuous source of novel chemicals and bioactive compounds due to illustrious history of use in traditional medicine. Research on Nepalese medicinal plants are still limited to ethnopharmacological studies and" /> <meta name="twitter:image" content="https://0.academia-photos.com/1574478/552688/689726/s200_janardan.lamichhane.jpg" /> <meta property="fb:app_id" content="2369844204" /> <meta property="og:type" content="article" /> <meta property="og:url" content="https://www.academia.edu/68898159/Phytochemical_profiling_using_HRLCMS_and_evaluation_of_antioxidant_and_antibacterial_activities_of_Nepalese_medicinal_plants" /> <meta property="og:title" content="Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants" /> <meta property="og:image" content="http://a.academia-assets.com/images/open-graph-icons/fb-paper.gif" /> <meta property="og:description" content="Medicinal plants have been conspicuous source of novel chemicals and bioactive compounds due to illustrious history of use in traditional medicine. Research on Nepalese medicinal plants are still limited to ethnopharmacological studies and" /> <meta property="article:author" content="https://sunmoon.academia.edu/JanardanLamichhane" /> <meta name="description" content="Medicinal plants have been conspicuous source of novel chemicals and bioactive compounds due to illustrious history of use in traditional medicine. Research on Nepalese medicinal plants are still limited to ethnopharmacological studies and" /> <title>(PDF) Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants | Janardan Lamichhane - Academia.edu</title> <link rel="canonical" href="https://www.academia.edu/68898159/Phytochemical_profiling_using_HRLCMS_and_evaluation_of_antioxidant_and_antibacterial_activities_of_Nepalese_medicinal_plants" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "single_work", 'action': "show", 'controller_action': 'single_work#show', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script> var $controller_name = 'single_work'; var $action_name = "show"; var $rails_env = 'production'; var $app_rev = '9387f500ddcbb8d05c67bef28a2fe0334f1aafb8'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.require = { config: function() { return function() {} } } </script> <script> window.Aedu = window.Aedu || {}; window.Aedu.hit_data = null; window.Aedu.serverRenderTime = new Date(1733085236000); window.Aedu.timeDifference = new Date().getTime() - 1733085236000; </script> <script type="application/ld+json">{"@context":"https://schema.org","@type":"ScholarlyArticle","abstract":"Medicinal plants have been conspicuous source of novel chemicals and bioactive compounds due to illustrious history of use in traditional medicine. Research on Nepalese medicinal plants are still limited to ethnopharmacological studies and qualitative phytochemical screening with a very few studies exploring their biological activities. This study aims to investigate biological activities of these plants and identify bioactive compounds present in each extract. A phytochemical profile of methanolic extracts of selected medicinal plants was established using high resolution (HR)-LCMS. Antioxidant activities were determined using DPPH, ABTS and FRAP assays. Highest DPPH radical scavenging was shown by Padamchal (IC50 = 3.47 ± 0.09), ABTS radical were most efficiently quenched by Pashanbed (IC50 = 3.82 ± 0.63) and the highest reducing potential was shown by Nirbikhi (FRAP = 61.76 ± 2.29 equivalent µg Fe2+/ml). The antioxidant activities of Padamchal and Pashanbed was comparable to that...","author":[{"@context":"https://schema.org","@type":"Person","name":"Janardan Lamichhane"}],"contributor":[],"dateCreated":"2022-01-20","dateModified":null,"datePublished":"2020-01-01","headline":"Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants","inLanguage":"en","keywords":["Chemistry"],"locationCreated":null,"publication":"Vegetos","publisher":{"@context":"https://schema.org","@type":"Organization","name":null},"image":null,"thumbnailUrl":null,"url":"https://www.academia.edu/68898159/Phytochemical_profiling_using_HRLCMS_and_evaluation_of_antioxidant_and_antibacterial_activities_of_Nepalese_medicinal_plants","sourceOrganization":[{"@context":"https://schema.org","@type":"EducationalOrganization","name":"sunmoon"}]}</script><link rel="stylesheet" media="all" 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{"work":{"id":68898159,"created_at":"2022-01-20T16:51:07.052-08:00","from_world_paper_id":192704893,"updated_at":"2024-11-27T07:43:07.082-08:00","_data":{"abstract":"Medicinal plants have been conspicuous source of novel chemicals and bioactive compounds due to illustrious history of use in traditional medicine. Research on Nepalese medicinal plants are still limited to ethnopharmacological studies and qualitative phytochemical screening with a very few studies exploring their biological activities. This study aims to investigate biological activities of these plants and identify bioactive compounds present in each extract. A phytochemical profile of methanolic extracts of selected medicinal plants was established using high resolution (HR)-LCMS. Antioxidant activities were determined using DPPH, ABTS and FRAP assays. Highest DPPH radical scavenging was shown by Padamchal (IC50 = 3.47 ± 0.09), ABTS radical were most efficiently quenched by Pashanbed (IC50 = 3.82 ± 0.63) and the highest reducing potential was shown by Nirbikhi (FRAP = 61.76 ± 2.29 equivalent µg Fe2+/ml). The antioxidant activities of Padamchal and Pashanbed was comparable to that...","ai_title_tag":"Phytochemical Profiling and Bioactivity of Nepalese Medicinal Plants","publication_date":"2020,,","publication_name":"Vegetos"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [1574478]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":79206839,"attachmentType":"pdf"}"><img alt="First page of “Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/79206839/mini_magick20220120-5160-cxbhyc.png?1642726391" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/assets/single_work_splash/adobe.icon-574afd46eb6b03a77a153a647fb47e30546f9215c0ee6a25df597a779717f9ef.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="1574478" href="https://sunmoon.academia.edu/JanardanLamichhane"><img alt="Profile image of Janardan Lamichhane" class="ds-work-card--author-avatar" src="https://0.academia-photos.com/1574478/552688/689726/s65_janardan.lamichhane.jpg" />Janardan Lamichhane</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2020, Vegetos</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">13 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 68898159; const worksViewsPath = "/v0/works/views?subdomain_param=api&work_ids%5B%5D=68898159"; const getWorkViews = async (workId) => { const response = await fetch(worksViewsPath); if (!response.ok) { throw new Error('Failed to load work views'); } const data = await response.json(); return data.views[workId]; }; // Get the view count for the work - we send this immediately rather than waiting for // the DOM to load, so it can be available as soon as possible (but without holding up // the backend or other resource requests, because it's a bit expensive and not critical). const viewCount = await getWorkViews(workId); const updateViewCount = (viewCount) => { const viewCountNumber = Number(viewCount); if (!viewCountNumber) { throw new Error('Failed to parse view count'); } const commaizedViewCount = viewCountNumber.toLocaleString(); const viewCountBody = document.getElementById('work-metadata-view-count'); if (viewCountBody) { viewCountBody.textContent = `${commaizedViewCount} views`; } else { throw new Error('Failed to find work views element'); } }; // If the DOM is still loading, wait for it to be ready before updating the view count. if (document.readyState === "loading") { document.addEventListener('DOMContentLoaded', () => { updateViewCount(viewCount); }); // Otherwise, just update it immediately. } else { updateViewCount(viewCount); } })();</script></div><p class="ds-work-card--work-abstract ds-work-card--detail ds2-5-body-md">Medicinal plants have been conspicuous source of novel chemicals and bioactive compounds due to illustrious history of use in traditional medicine. Research on Nepalese medicinal plants are still limited to ethnopharmacological studies and qualitative phytochemical screening with a very few studies exploring their biological activities. This study aims to investigate biological activities of these plants and identify bioactive compounds present in each extract. A phytochemical profile of methanolic extracts of selected medicinal plants was established using high resolution (HR)-LCMS. Antioxidant activities were determined using DPPH, ABTS and FRAP assays. Highest DPPH radical scavenging was shown by Padamchal (IC50 = 3.47 ± 0.09), ABTS radical were most efficiently quenched by Pashanbed (IC50 = 3.82 ± 0.63) and the highest reducing potential was shown by Nirbikhi (FRAP = 61.76 ± 2.29 equivalent µg Fe2+/ml). The antioxidant activities of Padamchal and Pashanbed was comparable to that...</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":79206839,"attachmentType":"pdf","workUrl":"https://www.academia.edu/68898159/Phytochemical_profiling_using_HRLCMS_and_evaluation_of_antioxidant_and_antibacterial_activities_of_Nepalese_medicinal_plants"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":79206839,"attachmentType":"pdf","workUrl":"https://www.academia.edu/68898159/Phytochemical_profiling_using_HRLCMS_and_evaluation_of_antioxidant_and_antibacterial_activities_of_Nepalese_medicinal_plants"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div></div><div data-auto_select="false" data-client_id="331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b" data-doc_id="79206839" data-landing_url="https://www.academia.edu/68898159/Phytochemical_profiling_using_HRLCMS_and_evaluation_of_antioxidant_and_antibacterial_activities_of_Nepalese_medicinal_plants" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="69621045" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/69621045/Evaluation_of_Phytochemical_Antioxidant_and_Antibacterial_Activities_of_Selected_Medicinal_Plants">Evaluation of Phytochemical, Antioxidant and Antibacterial Activities of Selected Medicinal Plants</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="158582682" href="https://cpds-tu.academia.edu/BishnuMarasini">Bishnu P Marasini</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2021</p><p class="ds-related-work--abstract ds2-5-body-sm">Medicinal plants are important reservoirs of bioactive compounds that need to be explored systematically. Because of their chemical diversity, natural products provide limitless possibilities for new drug discovery. This study aimed to investigate the biochemical properties of crude extracts from fifteen Nepalese medicinal plants. The total phenolic contents (TPC), total flavonoid contents (TFC), and antioxidant activity were evaluated through a colorimetric approach while the antibacterial activities were studied through the measurement of the zone of inhibition (ZoI) by agar well diffusion method along with minimum inhibitory concentrations (MIC) by broth dilution method. The methanolic extracts of Acacia catechu and Eupoterium adenophorum showed the highest TPC (55.21 ± 11.09 mg GAE/gm) and TFC (10.23 ± 1.07 mg QE/gm) among the studied plant extracts. Acacia catechu showed effective antioxidant properties with an IC50 value of 1.3 μg/mL, followed by extracts of Myrica esculenta, ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Evaluation of Phytochemical, Antioxidant and Antibacterial Activities of Selected Medicinal Plants","attachmentId":79645970,"attachmentType":"pdf","work_url":"https://www.academia.edu/69621045/Evaluation_of_Phytochemical_Antioxidant_and_Antibacterial_Activities_of_Selected_Medicinal_Plants","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/69621045/Evaluation_of_Phytochemical_Antioxidant_and_Antibacterial_Activities_of_Selected_Medicinal_Plants"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="72984339" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/72984339/Assessment_of_phytochemical_content_antioxidant_and_antibacterial_activities_of_three_medicinal_plants_of_Nepal">Assessment of phytochemical content, antioxidant and antibacterial activities of three medicinal plants of Nepal</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="20259671" href="https://independent.academia.edu/SubbaBimala">Bimala Subba</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Medicinal Plants Research</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Assessment of phytochemical content, antioxidant and antibacterial activities of three medicinal plants of Nepal","attachmentId":81688062,"attachmentType":"pdf","work_url":"https://www.academia.edu/72984339/Assessment_of_phytochemical_content_antioxidant_and_antibacterial_activities_of_three_medicinal_plants_of_Nepal","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/72984339/Assessment_of_phytochemical_content_antioxidant_and_antibacterial_activities_of_three_medicinal_plants_of_Nepal"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="30432985" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/30432985/Anti_oxidant_and_anti_microbial_properties_of_some_ethno_therapeutically_important_medicinal_plants_of_Indian_Himalayan_Region">Anti-oxidant and anti-microbial properties of some ethno-therapeutically important medicinal plants of Indian Himalayan Region</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="16900779" href="https://independent.academia.edu/SandeepRawat38">Sandeep Rawat</a></div><p class="ds-related-work--abstract ds2-5-body-sm">Therapeutic potential of medicinal plants as a source of noble natural anti-oxidants and anti-microbial agents has been well recognised all across the globe. In this study, phenolic compounds, in vitro anti-oxidant activity and anti-microbial properties have been investigated in five Himalayan medicinal plants, (e.g., Acorus calamus, Habe-naria intermedia, Hedychium spicatum, Roscoea procera and Valeriana jatamansi) in different solvent systems. R. procera exhibited significantly (p \ 0.05) higher phenolics; while H. spicatum was rich in flavonoids and V. jatamansi in anti-oxidant activity. Also, R. procera and H. spicatum were found rich in gallic acid; V. jatamansi in catechin, hydrox-ylbenzoic acid and caffeic acid and H. intermedia in hydroxyl benzoic acid. Solvent systems showed species specific response for extraction of total flavonoids and anti-oxidant activity. All the extracts were found effective against different bacterial and fungal strains in a dose dependent manner and maximum antimicrobial activity was found in R. procera as compared to other species. All the plant extracts showed greater activity against bacterial strains as compared to fungal strains. The results of this study suggest that extract of these species can be used as natural anti-oxidant to reduce free radical mediated disorders and as natural alternative for food preservation.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Anti-oxidant and anti-microbial properties of some ethno-therapeutically important medicinal plants of Indian Himalayan Region","attachmentId":50876180,"attachmentType":"pdf","work_url":"https://www.academia.edu/30432985/Anti_oxidant_and_anti_microbial_properties_of_some_ethno_therapeutically_important_medicinal_plants_of_Indian_Himalayan_Region","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/30432985/Anti_oxidant_and_anti_microbial_properties_of_some_ethno_therapeutically_important_medicinal_plants_of_Indian_Himalayan_Region"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="23901414" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/23901414/Evaluation_of_Phenolic_Content_Variability_along_with_Antioxidant_Antimicrobial_and_Cytotoxic_Potential_of_Selected_Traditional_Medicinal_Plants_from_India">Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="15857494" href="https://mzu.academia.edu/GarimaSingh">Garima Singh</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="9398562" href="https://mzu.academia.edu/snachimuthu">senthilkumar nachimuthu</a></div><p class="ds-related-work--abstract ds2-5-body-sm">Plants have been used since ancient times as an important source of biologically active substances. The aim of the present study was to investigate the phytochemical constituents (flavonoids and phenolics), antioxidant potential, cytotoxicity against HepG2 (human hepato carcinoma) cancer cell lines, and the antimicrobial activity of the methanol extract of selected traditional medicinal plants collected from Mizoram, India. A number of phenolic compounds were detected using HPLC-DAD-ESI-TOF-MS, mainly Luteolin, Kaempferol, Myricetin, Gallic Acid, Quercetin and Rutin, some of which have been described for the first time in the selected plants. The total phenolic and flavonoid contents showed high variation ranging from 4.44 to 181.91 µg of Gallic Acid equivalent per milligram DW (GAE/mg DW) and 3.17 to 102.2 µg of Quercetin/mg, respectively. The antioxidant capacity was determined by DPPH (IC 50 values ranges from 34.22 to 131.4 µg/mL), ABTS (IC 50 values ranges from 24.08 to 513.4 µg/mL), and reducing power assays. Antimicrobial activity was assayed against gram positive (Staphylococcus aureus), gram negative (Escherichia coli, Pseudomonas aeruginosa), and yeast (Candida albicans) demonstrating that the methanol extracts of some plants were efficacious antimicrobial agents. Additionally, cytotoxicity was assessed on human hepato carcinoma (HepG2) cancer cell lines and found that the extracts of Albizia lebbeck, Dillenia indica, and Bombax ceiba significantly decreased the cell viability at low concentrations with IC 50 values of 24.03, 25.09, and 29.66 µg/mL, respectively. This is the first report of detection of phenolic compounds along with antimicrobial, antioxidant and cytotoxic potential of selected medicinal plants from India, which indicates that these plants might be valuable source for human and animal health.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India","attachmentId":44290393,"attachmentType":"pdf","work_url":"https://www.academia.edu/23901414/Evaluation_of_Phenolic_Content_Variability_along_with_Antioxidant_Antimicrobial_and_Cytotoxic_Potential_of_Selected_Traditional_Medicinal_Plants_from_India","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/23901414/Evaluation_of_Phenolic_Content_Variability_along_with_Antioxidant_Antimicrobial_and_Cytotoxic_Potential_of_Selected_Traditional_Medicinal_Plants_from_India"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="44874868" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/44874868/Comparative_Analysis_of_a_few_Indian_Medicinal_Plants_for_their_Antimicrobial_and_Antioxidant_Properties">Comparative Analysis of a few Indian Medicinal Plants for their Antimicrobial and Antioxidant Properties</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34217662" href="https://technoscienceacademy.academia.edu/IJSRSET">International Journal of Scientific Research in Science, Engineering and Technology IJSRSET</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Scientific Research in Science, Engineering and Technology, 2020</p><p class="ds-related-work--abstract ds2-5-body-sm">Plant-derived substances have been used since antiquity for therapeutic and other purposes. The present study was undertaken to make a comparative analysis of antimicrobial and antioxidant properties of 8 medicinal plants, which are used in traditional medicine system. Methanolic extracts of Centella asiatica (leaves), Mentha piperita (leaves), Calotropis gigantean (leaves), Vitex nigundo (leaves), Bauhinia racemosus (leaves), Emblica officinalis (fruit), Bauhinia purpurea (leaves), Asperagus racemosus (stem) were tested for anti-microbial effects using the standard Agar Diffusion method. Antioxidant attributes of above extracts were studied by testing their ability to scavenge DPPH and ABTS radicles. Our results indicated that all tested extracts, with the exception of Bauhinia purpurea, were effective against E. Coli, while Candida albicans was only sensitive to Asperagus racemosus. Among tested extracts, Bauhinia racemosus was most potent in exhibiting radical scavenging activity. Our data provides rationale for exploitation of these plants for antimicrobial and antioxidant attributes.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Comparative Analysis of a few Indian Medicinal Plants for their Antimicrobial and Antioxidant Properties","attachmentId":65388001,"attachmentType":"pdf","work_url":"https://www.academia.edu/44874868/Comparative_Analysis_of_a_few_Indian_Medicinal_Plants_for_their_Antimicrobial_and_Antioxidant_Properties","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/44874868/Comparative_Analysis_of_a_few_Indian_Medicinal_Plants_for_their_Antimicrobial_and_Antioxidant_Properties"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="36128809" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/36128809/Antioxidant_Activity_of_Selected_Medicinal_Plants_of_Nepal">Antioxidant Activity of Selected Medicinal Plants of Nepal</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="27753359" href="https://pokhara.academia.edu/grinsunsharma">grinsun sharma</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="44219640" href="https://rguhs.academia.edu/BiswashSapkota">Biswash Sapkota</a></div><p class="ds-related-work--abstract ds2-5-body-sm">Objective The objective of the study was to investigate the antioxidant activity of selected medicinal plants. Method Collected plant species were subjected to maceration in methanol for 72 hrs. Antioxidant activity of plant extracts was assessed by using DPPH free radical scavenging method in different concentrations (1 µg/ml, 3 µg/ml, 5 µg/ ml, 7 µg/ml and 10 µg/ml) and percentage inhibition and effective concentration (EC50) values were calculated. Result Result indicates that, EC50 of D. boryanum (3.75 µg/ml) and P. guajava (3.89 µg/ml) was less, EC50 of R. nepalensis (5.03 µg/ml) and S. japonica (6.75 µg/ml) was comparable and EC50 M. macrophylla (7.86 µg/ml), B. asiatica (9.14 µg/ml), E. adenophorum (7.78 µg/ml), E. crassipes (8.21 µg/ml), and N. arbortritis (8.16 µg/ml) was slightly higher than ascorbic acid (4.73 µg/ml). Conclusion Our result shows that, D. boryanum and P. gvajava possess higher antioxidant activity than the ascorbic acid implying that, they could be good free radical scavenging agents and should be urbanized as a future pharmaceutical agent.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Antioxidant Activity of Selected Medicinal Plants of Nepal","attachmentId":56021337,"attachmentType":"pdf","work_url":"https://www.academia.edu/36128809/Antioxidant_Activity_of_Selected_Medicinal_Plants_of_Nepal","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/36128809/Antioxidant_Activity_of_Selected_Medicinal_Plants_of_Nepal"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="8572053" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/8572053/T%C3%9CB_TAK_Antioxidant_and_Free_Radical_Scavenging_Properties_of_Twelve_Traditionally_Used_Indian_Medicinal_Plants">TÜB‹TAK Antioxidant and Free Radical Scavenging Properties of Twelve Traditionally Used Indian Medicinal Plants</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="17689873" href="https://pustral-ugm.academia.edu/mustikow">mustiko w</a></div><div 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class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="20820749" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/20820749/Phytochemical_analysis_and_comprehensive_evaluation_of_antimicrobial_and_antioxidant_properties_of_61_medicinal_plant_species">Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="17269041" href="https://hu-pk.academia.edu/ihsanulhaq">ihsan ul haq</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Arabian Journal of Chemistry, 2015</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species","attachmentId":42367932,"attachmentType":"pdf","work_url":"https://www.academia.edu/20820749/Phytochemical_analysis_and_comprehensive_evaluation_of_antimicrobial_and_antioxidant_properties_of_61_medicinal_plant_species","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/20820749/Phytochemical_analysis_and_comprehensive_evaluation_of_antimicrobial_and_antioxidant_properties_of_61_medicinal_plant_species"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="79623751" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/79623751/In_vitro_antioxidative_potential_of_methanolic_aerial_extracts_from_three_ethnomedicinal_plants_of_Assam_A_Comparative_Study">In-vitro antioxidative potential of methanolic aerial extracts from three ethnomedicinal plants of Assam: A Comparative Study</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="189840811" href="https://independent.academia.edu/kabitamahato1">kabita mahato</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Applied Pharmaceutical Science, 2015</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"In-vitro antioxidative potential of methanolic aerial extracts from three ethnomedicinal plants of Assam: A Comparative Study","attachmentId":86275098,"attachmentType":"pdf","work_url":"https://www.academia.edu/79623751/In_vitro_antioxidative_potential_of_methanolic_aerial_extracts_from_three_ethnomedicinal_plants_of_Assam_A_Comparative_Study","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/79623751/In_vitro_antioxidative_potential_of_methanolic_aerial_extracts_from_three_ethnomedicinal_plants_of_Assam_A_Comparative_Study"><span class="ds2-5-text-link__content">View PDF</span><span 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the fractions were determined in comparison to positive controls such as quercetin with EC50 = 4.12±1.27, ascorbic acid with EC50 = 6.20±1.67, gallic acid with EC50 = 4.75±1.24 and α-tocopherol with EC50 = 32.50±1.57 μg/mL. The experiment showed that aqueous fractions of the bark extracts of Abies pindrow (fraction: C2) and Cedrus deodara (fraction: E2) showed significantly lower EC50 values of 2.5±0.5 and 2.5±0.6 μg/mL, respectively. In reducing power assay, lower EC50 values of 5.5 and 4.5μg/mL were recorded for the aqueous fraction (fraction: C 2) and final residue (fraction: C5), of Abies pindrow, respectively. The ethyl acetate, acetone and final fractions of knot wood of Picea smithiana were found significantly active against all bacterial strains. Of the most sensitive fractions towards all the fungal strains was ethyl acetate fraction obtained from the bark of Cedrus deodara with a zone of inhibition ranging from 75 to 88 % that was more than the standard fluconazole.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Evaluation of antioxidant and antimicrobial activities on various extracts of Himalayan medicinal plants","attachmentId":109509924,"attachmentType":"pdf","work_url":"https://www.academia.edu/112209641/Evaluation_of_antioxidant_and_antimicrobial_activities_on_various_extracts_of_Himalayan_medicinal_plants","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/112209641/Evaluation_of_antioxidant_and_antimicrobial_activities_on_various_extracts_of_Himalayan_medicinal_plants"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":79206839,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":79206839,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_79206839" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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