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lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>ChIP Kits & Reagents</a><a href="/browse?categories=Flow%20Cytometry%20Kits%20%26%20Reagents" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Flow Cytometry Kits & Reagents</a><a href="/browse?categories=Recombinant%20Proteins" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Recombinant Proteins</a><a href="/browse?categories=siRNA" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>siRNA</a><a href="/browse?categories=Cytokines%20%26%20Growth%20Factors" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Cytokines & Growth Factors</a><a href="/browse?categories=Activators%20%26%20Inhibitors" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Activators & Inhibitors</a><a href="/browse?categories=Buffers%20%26%20Dyes" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Buffers & Dyes</a><a href="/browse?formulation=BSA%20and%20Azide%20Free" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>BSA and Azide Free</a></div></div></div></div></div></li><li class="tw-group tw-px-[0.05em] lg:tw-relative nav-item-1" data-v-006bc605><a href="/applications" data-test-id="navigation-main_applications" class="navigation-main-link tw-hidden tw-p-4 lg:tw-block lg:tw-border-b-2 lg:tw-border-transparent lg:tw-py-2 lg:tw-px-0 lg:tw-uppercase lg:group-hover:tw-border-midnight-blue-500" data-v-006bc605><span class="tw-border-dusty-gray-300 lg:tw-border-r lg:tw-p-0 lg:tw-px-2 lg:group-last:tw-border-r-0" data-v-006bc605> Applications </span></a> <div class="tw-flex tw-cursor-pointer tw-items-center tw-justify-between tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 lg:tw-hidden" data-v-006bc605><span data-v-006bc605> Applications </span> <i aria-hidden="true" class="cstf cstf-angle-right tw-text-2xl" data-v-006bc605></i></div> <div class="lg:tw-hidden" style="display:none" data-v-006bc605><button class="tw-block tw-h-8 tw-w-full tw-text-left" data-v-006bc605><i aria-hidden="true" class="cstf cstf-angle-left tw-px-4 tw-text-2xl" data-v-006bc605></i></button> <a href="/applications" class="tw-block tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 tw-text-xl tw-font-bold tw-text-midnight-blue-500" data-v-006bc605> Applications </a></div> <div data-test-id="navigation-submenu_applications" class="menu-dropdown tw-bg-white lg:tw-absolute lg:tw-z-50 lg:tw-border-t-2 lg:tw-border-midnight-blue-500 lg:tw-shadow-lg" style="display:none" data-v-006bc605><div class="tw-flex tw-flex-col lg:tw-flex-row" data-v-006bc605> <div data-test-id="navigation-submenuSection_resource-centers" class="tw-flex tw-flex-col lg:tw-border-r lg:tw-py-2" data-v-006bc605><div class="tw-bg-dusty-gray-500 tw-px-2.5 tw-py-1.5 tw-text-xs tw-uppercase tw-text-white lg:tw-mx-2.5 lg:tw-mb-2.5" data-v-006bc605> Resource Centers </div>  <div class="menu-section tw-flex tw-flex-col lg:tw-flex-row resource-centers" data-v-006bc605><div class="tw-flex tw-flex-col tw-flex-wrap" data-v-006bc605><a href="/applications/chip-and-chip-seq" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>ChIP & ChIP-seq</a><a href="/applications/cut-and-run" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>CUT&RUN</a><a href="/applications/cut-tag" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>CUT&Tag</a><a href="/applications/elisa" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>ELISA</a><a href="/applications/flow-cytometry" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Flow Cytometry</a><a href="/applications/immunofluorescence" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Immunofluorescence</a><a href="/applications/immunohistochemistry" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Immunohistochemistry</a><a href="/applications/immunoprecipitation" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Immunoprecipitation</a><a href="/applications/intraseq-single-cell" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>InTraSeq™ Single Cell Analysis</a><a href="/applications/proteomics" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>LC/MS Proteomics</a><a href="/applications/multiplexing-and-spatial-biology" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Multiplexing and Spatial Biology</a><a href="/applications/signalstar-multiplex-ihc" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>SignalStar™ Multiplex IHC</a><a href="/applications/western-blot-immunoblot" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Western Blot (Immunoblot)</a></div></div></div></div></div></li><li class="tw-group tw-px-[0.05em] lg:tw-relative nav-item-2" data-v-006bc605><a href="/research" data-test-id="navigation-main_research" class="navigation-main-link tw-hidden tw-p-4 lg:tw-block lg:tw-border-b-2 lg:tw-border-transparent lg:tw-py-2 lg:tw-px-0 lg:tw-uppercase lg:group-hover:tw-border-midnight-blue-500" data-v-006bc605><span class="tw-border-dusty-gray-300 lg:tw-border-r lg:tw-p-0 lg:tw-px-2 lg:group-last:tw-border-r-0" data-v-006bc605> Research </span></a> <div class="tw-flex tw-cursor-pointer tw-items-center tw-justify-between tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 lg:tw-hidden" data-v-006bc605><span data-v-006bc605> Research </span> <i aria-hidden="true" class="cstf cstf-angle-right tw-text-2xl" data-v-006bc605></i></div> <div class="lg:tw-hidden" style="display:none" data-v-006bc605><button class="tw-block tw-h-8 tw-w-full tw-text-left" data-v-006bc605><i aria-hidden="true" class="cstf cstf-angle-left tw-px-4 tw-text-2xl" data-v-006bc605></i></button> <a href="/research" class="tw-block tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 tw-text-xl tw-font-bold tw-text-midnight-blue-500" data-v-006bc605> Research </a></div> <div data-test-id="navigation-submenu_research" class="menu-dropdown tw-bg-white lg:tw-absolute lg:tw-z-50 lg:tw-border-t-2 lg:tw-border-midnight-blue-500 lg:tw-shadow-lg" style="display:none" data-v-006bc605><div class="tw-flex tw-flex-col lg:tw-flex-row" data-v-006bc605><div data-test-id="navigation-submenuSection_research" class="menu-section tw-flex tw-flex-col tw-flex-wrap lg:tw-py-2 research" data-v-006bc605><a href="/research/cancer" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Cancer</a><a href="/research/cell-biology" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Cell Biology</a><a href="/research/developmental-biology-and-stem-cells" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Developmental Biology and Stem Cells</a><a href="/research/drug-discovery-solutions-powered-by-cst" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Drug Discovery Solutions Powered by CST</a><a href="/research/epigenetics" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Epigenetics</a><a href="/research/fibrosis" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Fibrosis</a><a href="/research/immunology-and-immuno-oncology" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Immunology and Immuno-Oncology</a><a href="/research/infectious-diseases" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Infectious Diseases / COVID-19</a><a href="/research/metabolism" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Metabolism</a><a href="/research/neuroscience" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Neuroscience</a><a href="/research/rna-regulation-and-translational-control" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>RNA Regulation and Translational Control</a></div> </div></div></li><li class="tw-group tw-px-[0.05em] lg:tw-relative nav-item-3" data-v-006bc605><a href="/pathways" data-test-id="navigation-main_pathways" class="navigation-main-link tw-hidden tw-p-4 lg:tw-block lg:tw-border-b-2 lg:tw-border-transparent lg:tw-py-2 lg:tw-px-0 lg:tw-uppercase lg:group-hover:tw-border-midnight-blue-500" data-v-006bc605><span class="tw-border-dusty-gray-300 lg:tw-border-r lg:tw-p-0 lg:tw-px-2 lg:group-last:tw-border-r-0" data-v-006bc605> Pathways </span></a> <div class="tw-flex tw-cursor-pointer tw-items-center tw-justify-between tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 lg:tw-hidden" data-v-006bc605><span data-v-006bc605> Pathways </span> <i aria-hidden="true" class="cstf cstf-angle-right tw-text-2xl" data-v-006bc605></i></div> <div class="lg:tw-hidden" style="display:none" data-v-006bc605><button class="tw-block tw-h-8 tw-w-full tw-text-left" data-v-006bc605><i aria-hidden="true" class="cstf cstf-angle-left tw-px-4 tw-text-2xl" data-v-006bc605></i></button> <a href="/pathways" class="tw-block tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 tw-text-xl tw-font-bold tw-text-midnight-blue-500" data-v-006bc605> Pathways </a></div> <div data-test-id="navigation-submenu_pathways" class="menu-dropdown tw-bg-white lg:tw-absolute lg:tw-z-50 lg:tw-border-t-2 lg:tw-border-midnight-blue-500 lg:tw-shadow-lg" style="display:none" data-v-006bc605><div class="tw-flex tw-flex-col lg:tw-flex-row" data-v-006bc605><div data-test-id="navigation-submenuSection_pathways" class="menu-section tw-flex tw-flex-col tw-flex-wrap lg:tw-py-2 pathways" data-v-006bc605><a href="/pathways/by-disease-area" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>By Disease Area</a><a href="/pathways/by-research-area" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>By Research Area</a><a href="/pathways/cellular-landscapes" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Cellular Landscapes</a></div> </div></div></li><li class="tw-group tw-px-[0.05em] lg:tw-relative nav-item-4" data-v-006bc605><a href="/services" data-test-id="navigation-main_services" class="navigation-main-link tw-hidden tw-p-4 lg:tw-block lg:tw-border-b-2 lg:tw-border-transparent lg:tw-py-2 lg:tw-px-0 lg:tw-uppercase lg:group-hover:tw-border-midnight-blue-500" data-v-006bc605><span class="tw-border-dusty-gray-300 lg:tw-border-r lg:tw-p-0 lg:tw-px-2 lg:group-last:tw-border-r-0" data-v-006bc605> Services </span></a> <div class="tw-flex tw-cursor-pointer tw-items-center tw-justify-between tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 lg:tw-hidden" data-v-006bc605><span data-v-006bc605> Services </span> <i aria-hidden="true" class="cstf cstf-angle-right tw-text-2xl" data-v-006bc605></i></div> <div class="lg:tw-hidden" style="display:none" data-v-006bc605><button class="tw-block tw-h-8 tw-w-full tw-text-left" data-v-006bc605><i aria-hidden="true" class="cstf cstf-angle-left tw-px-4 tw-text-2xl" data-v-006bc605></i></button> <a href="/services" class="tw-block tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 tw-text-xl tw-font-bold tw-text-midnight-blue-500" data-v-006bc605> Services </a></div> <div data-test-id="navigation-submenu_services" class="menu-dropdown tw-bg-white lg:tw-absolute lg:tw-z-50 lg:tw-border-t-2 lg:tw-border-midnight-blue-500 lg:tw-shadow-lg" style="display:none" data-v-006bc605><div class="tw-flex tw-flex-col lg:tw-flex-row" data-v-006bc605><div data-test-id="navigation-submenuSection_services" class="menu-section tw-flex tw-flex-col tw-flex-wrap lg:tw-py-2 services" data-v-006bc605><a href="/services/customized-and-carrier-free-formulation" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Carrier-free Custom Antibody Formulations</a><a href="/services/custom-peptides" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>AQUA and Custom Blocking Peptides</a><a href="/services/proteomics-analytical-services" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Proteomics Analytical Services</a><a href="/services/custom-conjugation-services" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Custom Conjugation & Labeling Services</a><a href="/services/bulk-quantities-and-lot-reservations" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Bulk Quantities and Lot Reservations</a></div> </div></div></li><li class="tw-group tw-px-[0.05em] 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href="/learn-and-support" class="tw-block tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 tw-text-xl tw-font-bold tw-text-midnight-blue-500" data-v-006bc605> Learn & Support </a></div> <div data-test-id="navigation-submenu_learn-support" class="menu-dropdown tw-bg-white lg:tw-absolute lg:tw-z-50 lg:tw-border-t-2 lg:tw-border-midnight-blue-500 lg:tw-shadow-lg" style="display:none" data-v-006bc605><div class="tw-flex tw-flex-col lg:tw-flex-row" data-v-006bc605> <div data-test-id="navigation-submenuSection_education-resources" class="tw-flex tw-flex-col lg:tw-border-r lg:tw-py-2" data-v-006bc605><div class="tw-bg-dusty-gray-500 tw-px-2.5 tw-py-1.5 tw-text-xs tw-uppercase tw-text-white lg:tw-mx-2.5 lg:tw-mb-2.5" data-v-006bc605> Education & Resources </div>  <div class="menu-section tw-flex tw-flex-col lg:tw-flex-row education-resources" data-v-006bc605><div class="tw-flex tw-flex-col tw-flex-wrap" data-v-006bc605><a href="/learn-and-support/antibody-conjugates" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Antibody Conjugates</a><a href="https://blog.cellsignal.com/" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Blog</a><a href="/learn-and-support/conferences-and-events" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Conferences & Events</a><a href="/learn-and-support/control-treatments-by-target" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Control Treatments by Target</a><a href="/learn-and-support/pdf-download-page" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Downloadable Literature</a><a href="/learn-and-support/phosphositeplus-ptm-database" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>PhosphoSitePlus® PTM Database</a></div><div class="tw-flex tw-flex-col tw-flex-wrap" data-v-006bc605><a href="/learn-and-support/protein-domains-and-interactions" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Protein Domains & Interactions</a><a href="/learn-and-support/protein-kinases" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Protein Kinases</a><a href="/learn-and-support/publications-and-posters" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Publications & Research Posters</a><a href="/learn-and-support/reference-tables" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Reference Tables</a><a href="/learn-and-support/scientific-web-resources" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Scientific Web Resources</a><a href="/learn-and-support/videos-and-webinars" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Videos & Webinars</a></div></div></div><div data-test-id="navigation-submenuSection_troubleshooting-support" class="tw-flex tw-flex-col lg:tw-border-r lg:tw-py-2" data-v-006bc605><div class="tw-bg-dusty-gray-500 tw-px-2.5 tw-py-1.5 tw-text-xs tw-uppercase tw-text-white lg:tw-mx-2.5 lg:tw-mb-2.5" data-v-006bc605> Troubleshooting & Support </div>  <div class="menu-section tw-flex tw-flex-col lg:tw-flex-row troubleshooting-support" data-v-006bc605><div class="tw-flex tw-flex-col tw-flex-wrap" data-v-006bc605><a href="/learn-and-support/technical-support" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Technical Support</a><a href="/learn-and-support/support-information" class="tw-block tw-border-b tw-border-l-4 tw-border-dusty-gray-300 tw-border-l-transparent tw-px-3 tw-py-2 hover:tw-border-l-midnight-blue-500 hover:tw-text-midnight-blue-500 lg:tw-mx-2.5 lg:tw-border-0 lg:tw-border-l-4 lg:tw-border-transparent lg:tw-p-2 lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Contact Support</a><a href="/learn-and-support/troubleshooting" class="tw-block tw-border-b 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lg:hover:tw-border-midnight-blue-500" data-v-006bc605>Technical Documentation</a></div></div></div></div></div></li><li class="tw-group tw-px-[0.05em] lg:tw-relative nav-item-6" data-v-006bc605><a href="/about-us" data-test-id="navigation-main_about-us" class="navigation-main-link tw-hidden tw-p-4 lg:tw-block lg:tw-border-b-2 lg:tw-border-transparent lg:tw-py-2 lg:tw-px-0 lg:tw-uppercase lg:group-hover:tw-border-midnight-blue-500" data-v-006bc605><span class="tw-border-dusty-gray-300 lg:tw-border-r lg:tw-p-0 lg:tw-px-2 lg:group-last:tw-border-r-0" data-v-006bc605> About us </span></a> <div class="tw-flex tw-cursor-pointer tw-items-center tw-justify-between tw-border-b tw-border-dusty-gray-300 tw-px-4 tw-py-2 lg:tw-hidden" data-v-006bc605><span data-v-006bc605> About us </span> <i aria-hidden="true" class="cstf cstf-angle-right tw-text-2xl" data-v-006bc605></i></div> <div class="lg:tw-hidden" style="display:none" data-v-006bc605><button class="tw-block tw-h-8 tw-w-full tw-text-left" 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src="https://media.cellsignal.com/cdn-cgi/imagedelivery/JWox9JU8xLM9DWO4l_Iqsw/56ba266a-12ed-4284-c9ce-42a53e14f400/1X" alt="1% for the planet logo" class="tw-max-h-full tw-max-w-full"></a> <div class="tw-z-20 tw-w-full tw-basis-9/12 md:tw-basis-8/12"><div class="tw-relative tw-box-border tw-flex tw-h-10 tw-flex-row tw-items-stretch tw-rounded-sm tw-border tw-border-dusty-gray md:tw-h-12" data-v-002b971a><input tabindex="0" aria-label="search input field" data-test-id="searchInputField-input" maxlength="128" class="placeholder:tw-text-sm" data-v-002b971a>  <button type="submit" id="global-search-btn" aria-label="search" title="search" class="inactive" data-v-965690b0 data-v-002b971a><i class="cstf cstf-search" data-v-965690b0 data-v-002b971a></i></button></div> </div></div></div></div> </section></header> <main class="tw-container tw-mx-auto tw-max-w-screen-lg tw-py-2 tw-px-4 lg:tw-px-2"><div id="main_template"><div data-test-id="cstSpinner-global" class="tw-fixed tw-top-0 tw-left-0 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Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). 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data-v-29c18229><span itemprop="name" data-v-29c18229>Antibody Conjugates</span></a> <meta itemprop="position" content="2" data-v-29c18229> <span class="divider" data-v-29c18229>/</span></li><li itemscope itemtype="http://schema.org/ListItem" itemprop="itemListElement" data-v-29c18229><span itemprop="name" data-v-29c18229>Phospho-Histone H3 (Ser10) (D2C8) XP<sup>®</sup> Rabbit mAb (Alexa Fluor<sup>®</sup> 647 Conjugate)</span> <meta itemprop="position" content="3" data-v-29c18229> <meta itemprop="item" content="" data-v-29c18229></li></ol> <div class="recombinant-flag" data-v-0bc1a06e><div id="recombinant-flag-rectangle" title="Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing." data-v-0bc1a06e><span id="left-side" data-v-0bc1a06e>R</span> <span id="right-side" data-v-0bc1a06e>Recombinant</span></div> <div data-test-id="recombinant-tooltip" class="recombinant-tooltip" data-v-0bc1a06e><svg viewBox="0 0 512 512" width="1.2em" height="1.2em" data-v-0bc1a06e><path fill="currentColor" d="M256 8C119.043 8 8 119.083 8 256c0 136.997 111.043 248 248 248s248-111.003 248-248C504 119.083 392.957 8 256 8zm0 110c23.196 0 42 18.804 42 42s-18.804 42-42 42s-42-18.804-42-42s18.804-42 42-42zm56 254c0 6.627-5.373 12-12 12h-88c-6.627 0-12-5.373-12-12v-24c0-6.627 5.373-12 12-12h12v-64h-12c-6.627 0-12-5.373-12-12v-24c0-6.627 5.373-12 12-12h64c6.627 0 12 5.373 12 12v100h12c6.627 0 12 5.373 12 12v24z"></path></svg> <span data-test-id="recombinant-tooltip-text" class="recombinant-tooltiptext" data-v-0bc1a06e><b data-v-0bc1a06e>Recombinant:</b> Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing. </span></div> </div>  <div class="title" data-v-60b43a68><h1 class="tw-my-3 tw-text-2xl" data-v-60b43a68> <span data-v-60b43a68>Phospho-Histone H3 (Ser10) (D2C8) XP<sup>®</sup> Rabbit mAb (Alexa Fluor<sup>®</sup> 647 Conjugate)</span> #3458 </h1></div>  <button class="citation-count tw-mb-2.5" data-v-257e77bc><i class="cstf 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<div class="tw-animate-pulse tw-bg-gray-300 tw-h-7 tw-w-full tw-animate-pulse tw-rounded-md tw-bg-gray-300"></div></div></div></div></div></section></div><div class="md:tw-flex md:tw-flex-col"><section><div class="tw-flex tw-flex-col md:tw-basis-1/1">  <section data-component="InPageNavigationPdpWrapper" templatecomponentdata="[object Object]"><div id="mobile-navigation" class="md:tw-hidden"><div class="tw-fixed tw-top-0 tw-left-0 tw-z-[50] tw-h-full tw-w-full tw-bg-[#ffffffe6]" style="display:none"><ul class="tw-fixed tw-right-7 tw-bottom-24" data-v-2b86a439></ul></div> <div class="tw-fixed tw-bottom-5 tw-right-5 tw-z-[50] tw-flex tw-items-center tw-gap-2"><p class="tw-text-base tw-text-dusty-gray-900" style="display:none">On Page Menu</p> <div class="tw-flex tw-h-14 tw-w-14 tw-items-center tw-justify-center tw-rounded-full tw-bg-danube tw-text-lg tw-text-white tw-shadow-[4px_4px_8px_0_rgba(0,0,0,.45)]"><i class="cstf cstf-protocols tw-text-3xl"></i></div></div></div> <div><div id="in-page-navigation" class="tw-w-full tw-bg-white tw-py-2 tw-z-10 tw-hidden tw-h-24 md:tw-block !tw-hidden" style="width:undefinedpx"><ul data-v-2b86a439></ul></div></div></section></div></section></div><div class="md:tw-flex md:tw-flex-col"><section class="tw-flex tw-flex-col tw-gap-8 md:tw-flex-row"><div class="tw-flex tw-flex-col md:tw-basis-2/3">  <div id="supporting-data" data-component="SupportingDataPdpWrapper" class="supporting-data tw-scroll-mt-52" data-v-f4c72556><h3 data-v-f4c72556>Supporting Data</h3> <table data-v-f4c72556><tbody data-v-f4c72556><tr data-v-f4c72556><td data-v-f4c72556>REACTIVITY</td> <td data-v-f4c72556>H M R Mk Z</td></tr><tr data-v-f4c72556><td data-v-f4c72556>SENSITIVITY</td> <td data-v-f4c72556>Endogenous</td></tr><tr data-v-f4c72556><td data-v-f4c72556>MW (kDa)</td> <td data-v-f4c72556></td></tr><tr data-v-f4c72556><td data-v-f4c72556>Source/Isotype</td> <td data-v-f4c72556>Rabbit IgG</td></tr></tbody></table> <div class="product-abbreviations" data-v-f4c72556><h5 data-v-f4c72556>Application Key:</h5> <ul data-v-f4c72556><li data-v-f4c72556><strong data-v-f4c72556>IF</strong>-Immunofluorescence </li><li data-v-f4c72556><strong data-v-f4c72556>F</strong>-Flow Cytometry </li></ul> <h5 data-v-f4c72556>Species Cross-Reactivity Key:</h5> <ul data-v-f4c72556><li data-v-f4c72556><strong data-v-f4c72556>H</strong>-Human </li><li data-v-f4c72556><strong data-v-f4c72556>M</strong>-Mouse </li><li data-v-f4c72556><strong data-v-f4c72556>R</strong>-Rat </li><li data-v-f4c72556><strong data-v-f4c72556>Mk</strong>-Monkey </li><li data-v-f4c72556><strong data-v-f4c72556>Z</strong>-Zebrafish </li></ul></div></div><div data-component="ProductListPdpWrapper" data-v-27f14d6b></div><div data-component="ProductInformationPdpWrapper" class="product-information" data-v-7e9802d4><h2 data-v-7e9802d4>Product Information</h2> <div class="description-component" data-v-c8c44b8a data-v-7e9802d4><h3 data-v-c8c44b8a>Product Description</h3> <div class="tw-mb-3" data-v-c8c44b8a>This Cell Signaling Technology antibody is conjugated to Alexa Fluor<sup>®</sup> 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Histone H3 (Ser10) (D2C8) XP<sup>®</sup> Rabbit mAb #3377.</div> <table data-v-c8c44b8a><tbody data-v-c8c44b8a>      </tbody></table></div><div id="product-usage" class="tw-scroll-mt-52" data-v-181bd034 data-v-7e9802d4><div class="border-bottom" data-v-181bd034><h3 data-v-181bd034>Product Usage Information</h3>  <table class="tw-w-full tw-text-left tw-leading-8" data-v-181bd034><thead data-v-181bd034><tr data-v-181bd034><th data-v-181bd034>Application</th> <th data-v-181bd034>Dilution</th></tr></thead> <tbody data-v-181bd034><tr data-v-181bd034><td data-v-181bd034>Immunofluorescence (Immunocytochemistry)</td> <td data-v-181bd034>1:400</td></tr><tr data-v-181bd034><td data-v-181bd034>Flow Cytometry (Fixed/Permeabilized)</td> <td data-v-181bd034>1:50</td></tr></tbody></table> </div>   <h3 data-v-181bd034>Storage</h3> <div data-v-181bd034>Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.</div></div><div id="protocols" class="tw-scroll-mt-52 protocol-component" data-v-bb7b31e0 data-v-7e9802d4><h3 data-v-bb7b31e0>Protocol</h3> <select class="tw-max-w-[16rem] tw-rounded-none tw-border-black tw-p-0" data-v-bb7b31e0><option selected data-v-bb7b31e0>Select your Protocol</option> <option value="182" data-v-bb7b31e0> Immunofluorescence (Immunocytochemistry) </option><option value="407" data-v-bb7b31e0> Flow Cytometry (Fixed/Permeabilized) </option></select>    </div><div class="specificity-or-sensitivity" data-v-24efef65 data-v-7e9802d4><h3 data-v-24efef65>Specificity / Sensitivity</h3> <div class="specificity-description" data-v-24efef65><p>Phospho-Histone H3 (Ser10) (D2C8) XP<sup>®</sup> Rabbit mAb (Alexa Fluor<sup>®</sup> 647 Conjugate) detects endogenous levels of histone H3 only when phosphorylated at Ser10. The antibody does not cross-react with other phosphorylated histones or with acetylated histones.</p><p> </p><p>Lack of staining in G2 cells has been reported in some cell lines by immunofluorescence.</p></div> <br data-v-24efef65> <p class="tw-font-bold" data-v-24efef65>Species Reactivity:</p> <div data-v-24efef65>Human, Mouse, Rat, Monkey, Zebrafish</div> </div><div class="source-or-purification" data-v-3da27cd4 data-v-7e9802d4><h3 data-v-3da27cd4>Source / Purification</h3> <div data-v-3da27cd4>Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3. This antibody was conjugated to Alexa Fluor<sup>®</sup> 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor<sup>®</sup> 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor<sup>®</sup> 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.</div></div><div id="background" class="tw-scroll-mt-52" data-v-969bc690 data-v-7e9802d4><h3 data-v-969bc690>Background</h3> <div class="tw-mb-2.5" data-v-969bc690>Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).</div> <div data-v-969bc690><ol class="OneLinkNoTx tw-pl-10" data-v-969bc690><li data-test-id="background-references_'3458_GROUP_1'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/9759497" target="_blank" class="anchorLink" data-v-969bc690>Workman, J.L. and Kingston, R.E. (1998) <cite>Annu Rev Biochem</cite> 67, 545-79.</a></li><li data-test-id="background-references_'3458_GROUP_2'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/9922128" target="_blank" class="anchorLink" data-v-969bc690>Hansen, J.C. et al. (1998) <cite>Biochemistry</cite> 37, 17637-41.</a></li><li data-test-id="background-references_'3458_GROUP_3'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/10638745" target="_blank" class="anchorLink" data-v-969bc690>Strahl, B.D. and Allis, C.D. (2000) <cite>Nature</cite> 403, 41-5.</a></li><li data-test-id="background-references_'3458_GROUP_4'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/11057899" target="_blank" class="anchorLink" data-v-969bc690>Cheung, P. et al. (2000) <cite>Cell</cite> 103, 263-71.</a></li><li data-test-id="background-references_'3458_GROUP_5'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/12445767" target="_blank" class="anchorLink" data-v-969bc690>Bernstein, B.E. and Schreiber, S.L. (2002) <cite>Chem Biol</cite> 9, 1167-73.</a></li><li data-test-id="background-references_'3458_GROUP_6'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/12724776" target="_blank" class="anchorLink" data-v-969bc690>Jaskelioff, M. and Peterson, C.L. (2003) <cite>Nat Cell Biol</cite> 5, 395-9.</a></li><li data-test-id="background-references_'3458_GROUP_7'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/2249688" target="_blank" class="anchorLink" data-v-969bc690>Thorne, A.W. et al. (1990) <cite>Eur J Biochem</cite> 193, 701-13.</a></li><li data-test-id="background-references_'3458_GROUP_8'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/9362543" target="_blank" class="anchorLink" data-v-969bc690>Hendzel, M.J. et al. (1997) <cite>Chromosoma</cite> 106, 348-60.</a></li><li data-test-id="background-references_'3458_GROUP_9'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/10464286" target="_blank" class="anchorLink" data-v-969bc690>Goto, H. et al. (1999) <cite>J Biol Chem</cite> 274, 25543-9.</a></li><li data-test-id="background-references_'3458_GROUP_10'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/12560483" target="_blank" class="anchorLink" data-v-969bc690>Preuss, U. et al. (2003) <cite>Nucleic Acids Res</cite> 31, 878-85.</a></li><li data-test-id="background-references_'3458_GROUP_11'" data-v-969bc690><a href="http://www.ncbi.nlm.nih.gov/pubmed/15681610" target="_blank" class="anchorLink" data-v-969bc690>Dai, J. et al. (2005) <cite>Genes Dev</cite> 19, 472-88.</a></li></ol></div></div><div id="pathways" class="tw-scroll-mt-52 upstream-or-downstream-pathway-image" data-v-9177d07a data-v-7e9802d4></div><div class="database-links" data-v-08014b80 data-v-7e9802d4><h3 data-v-08014b80>Database Links</h3> <p data-v-08014b80> UniProt ID:  <a href="http://www.uniprot.org/uniprot/P68431" target="_blank" class="anchorLink" data-v-08014b80> P68431 </a></p> <br data-v-08014b80> <p data-v-08014b80> Entrez-Gene Id:  <a href="http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=8350" target="_blank" class="anchorLink" data-v-08014b80> 8350 </a></p> <br data-v-08014b80> <button class="anchorLink" data-v-08014b80> View in <img src="//media.cellsignal.com/www/images/project-images/img/assets/PHosphosite_Icon.png" width="22" height="22" alt="PhosphoSitePlus<sup>®</sup> logo" class="tw-inline-block" data-v-08014b80>PhosphoSitePlus<sup data-v-08014b80>®</sup></button></div></div><div id="citations" data-component="CitationsPdpWrapper" class="tw-scroll-mt-52" data-v-ab599df2></div><div data-test-id="legalStatement-container" data-component="LegalStatementPdpWrapper" templatecomponentdata="[object Object]" class="tw-mb-10" data-v-7ca4e51c><div class="OneLinkNoTx legal-statement" data-v-7ca4e51c><h4>Limited Uses</h4><p>Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. 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