SARS-CoV-2 Spike S1 Subunit Alexa Fluor® 350-conjugated Antibody (FAB105403U) by R&D Systems, Part of Bio-Techne

<!DOCTYPE html> <html lang="en" dir="ltr" prefix="og: https://ogp.me/ns#"> <head> <meta charset="utf-8" /> <noscript><style>form.antibot * :not(.antibot-message) { display: none !important; }</style> </noscript><meta name="applicable-device" content="pc,mobile" /> <link rel="canonical" href="https://www.bio-techne.com/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-350-conjugated-antibody-1035206_fab105403u" /> <meta property="og:site_name" content="Bio-Techne" /> <meta property="og:type" content="Website" /> <meta property="og:url" content="https://www.bio-techne.com/" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <script type="application/ld+json">{ "@context": "https://schema.org", "@graph": [ { "@type": "WebPage", "@id": "https://www.bio-techne.com/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-350-conjugated-antibody-1035206_fab105403u", "breadcrumb": { "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Home", "item": "https://www.bio-techne.com/" }, { "@type": "ListItem", "position": 2, "name": "Spike S1 Subunit", "item": "https://www.bio-techne.com/t/spike-s1-subunit" }, { "@type": "ListItem", "position": 3, "name": "Spike S1 Subunit Antibodies", "item": "https://www.bio-techne.com/t/spike-s1-subunit/antibodies" } ] } } ] }</script> <meta http-equiv="x-dns-prefetch-control" content="on" /> <meta http-equiv="x-ua-compatible" content="ie=edge" /> <meta name="keywords" content=", Mouse Anti-SARS-CoV-2 Spike S1 Subunit Alexa&nbsp;Fluor®&nbsp;350-conjugated Monoclonal Antibody, Mouse Anti SARS-CoV-2 Spike S1 Subunit Alexa&nbsp;Fluor®&nbsp;350-conjugated Monoclonal Antibody, Anti-Spike S1 Subunit" /> <meta name="description" content="View SARS-CoV-2 Spike S1 Subunit Alexa&nbsp;Fluor®&nbsp;350-conjugated Antibody (FAB105403U) validated in SARS-CoV-2 & Flow Cytometry from R&D Systems." /> <script type="application/ld+json" id="json-ld">[{"@context":"http://schema.org","@type":"Product","additionalProperty":[{"additionalProperty":{"@type":"PropertyValue","name":"Applications","value":"Flow Cytometry"}},{"additionalProperty":{"@type":"PropertyValue","name":"Immunogen","value":"HEK293-derived SARS-CoV-2 Spike S1 Subunit proteinAccession # YP_009724390.1"}},{"additionalProperty":{"@type":"PropertyValue","name":"Specificity","value":"Detects SARS-CoV-2 Spike S1 subunit in direct ELISAs and Western blots. 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class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup 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<div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main-onedate-product-pages" class="bg-white px-4 "> <div class="container"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> Products </li> <li> <a href="/t/spike-s1-subunit">Spike S1 Subunit</a> </li> <li> <a href="/t/spike-s1-subunit/antibodies">Spike S1 Subunit Antibodies</a> </li> <li> SARS-CoV-2 Spike S1 Subunit Alexa Fluor® 350-conjugated Antibody (FAB105403U) </li> </ol> </nav> </div> </div> </div> <div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <div id="product-page-details" data-template_in_use="antibody_template" data-time_total="0.78471302986145" data-time_to_compile="0.38375496864319" data-time_to_build="0.71973896026611" data-time_datapoints="-0.31878089904785" data-time_template="0.31878089904785" data-time_post_epcc="1.1034939289093" data-time_epcc="-0.31878089904785" data-render_time="Wednesday 30th of October 2024 12:09:18 PM" data-fallback="FALSE" data-time_to_process_data="0.41279482841492" class="px-4 container" class="container"> <div id="above-fold-section"> <div class="mb-2 o__product-summary"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> SARS-CoV-2 Spike S1 Subunit Alexa Fluor® 350-conjugated Antibody </h1> <h2 class="font-weight-normal h4"> R&D Systems, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">FAB105403U</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="submit_review_wrapper" class="col-auto d-flex align-self-center"> <div id="submit_review"> <a href="/reviews/FAB105403U" class="submit_review" rel="nofollow"> <span class="icon-plus-circle d-none d-lg-inline pr-1"></span> Submit a review </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <div class="px-0 my-1" id="datasheet-summary-wrapper" data-section="section-base--top-jumplink"> <article class="top-jumplink-wrapper"> <span class="icon-icon-pdf text-error-red"></span> <a href="#product-documents" class="product-documents-top-link" rel="nofollow">Product Datasheet / COA / SDS</a> </article> </div> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" alt="R&D Systems, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> <div class="row border-top mb-2" id="images-orderdetail-wrapper"> <div class="col-xl-6 mt-2" id="images-wrapper" data-section="image_summary"> <div id="image-tree" data-section="component-product-image-tree" class="vertical lightbox" data-mdb-zoom-effect="true"> <div id="product-image-gallery" class="row"> <div class="col-md-11 border rounded d-flex align-items-center justify-content-center product-image-lightbox"> <div class="text-center my-2" data-brand="rnd"> <img src="//resources.bio-techne.com/images/1bt/bio-techne-logo.png" alt="SARS-CoV-2 Spike S1 Subunit Alexa&nbsp;Fluor®&nbsp;350-conjugated Antibody" title="SARS-CoV-2 Spike S1 Subunit Alexa&nbsp;Fluor®&nbsp;350-conjugated Antibody" data-caption="SARS-CoV-2 Spike S1 Subunit Alexa&nbsp;Fluor®&nbsp;350-conjugated Antibody" data-badges='' data-id="0" width="222" height="143" fetchpriority="high" as="image" class="search_result_thumbnail_image active" /> </div> </div> </div> </div> </div> <div class="col-xl-6 mt-2" id="orderdetails-wrapper" data-section="order_detail_block"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="FAB105403U"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-3 p-0 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-2 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="FAB105403U-100UG" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">FAB105403U-100UG</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <div class="atc-control ProductCart-control text-right"> <div class="row container d-flex justify-content-end mx-0 mb-3 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="" class="atc-print-quote-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100">Print Quote</a> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=FAB105403U" class="atc-bulk-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> <div class="atc-container order_detail_wrapper conjugates-and-formulations-wrapper my-2 position-relative"> <div class="button-group text-right pl-2"> <a href="#conjugates-FAB105403U" class="btn btn-secondary atc-view-pricing atc-view-pricing-products collapsed text-nowrap mb-2" data-id="conjugates-FAB105403U" data-toggle="collapse" aria-expanded="false" aria-controls="conjugates">View Available Conjugates </a> </div> <div id="conjugates-FAB105403U" class="conjugates-and-formulations-container collapse position-absolute w-100"> <div class="atc-container border rounded bg-white shadow p-2 mt-1"> <div class="commerce-atc-datasheet atc-block-collapsible" data-type="conjugates_and_formulations" data-blockid="fab105403u-conjugate-atc-block"> <div id="fab105403u-conjugate-atc-block" class="atc-lines"> <div class="atc-header row pb-1 m-0"> <div class="atc-head col-4 p-0 font-weight-bold d-sm-none d-md-block">Conjugate</div> <div class="atc-head col-4 p-0 font-weight-bold">Catalog #</div> <div class="atc-head col-4"></div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="MAB105403" data-titleoverwrite="Unconjugated"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-antibody-1035206_mab105403">Unconjugated</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-antibody-1035206_mab105403">MAB105403</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-antibody-1035206_mab105403" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403V" data-titleoverwrite="Alexa Fluor 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-405-conjugated-antibody-1035206_fab105403v">Alexa Fluor 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-405-conjugated-antibody-1035206_fab105403v">FAB105403V</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-405-conjugated-antibody-1035206_fab105403v" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403G" data-titleoverwrite="Alexa Fluor 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-488-conjugated-antibody-1035206_fab105403g">Alexa Fluor 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-488-conjugated-antibody-1035206_fab105403g">FAB105403G</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-488-conjugated-antibody-1035206_fab105403g" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403X" data-titleoverwrite="Alexa Fluor 532"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403x">Alexa Fluor 532</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403x">FAB105403X</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403x" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403T" data-titleoverwrite="Alexa Fluor 594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-594-conjugated-antibody-1035206_fab105403t">Alexa Fluor 594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-594-conjugated-antibody-1035206_fab105403t">FAB105403T</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-594-conjugated-antibody-1035206_fab105403t" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403R" data-titleoverwrite="Alexa Fluor 647"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-647-conjugated-antibody-1035206_fab105403r">Alexa Fluor 647</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-647-conjugated-antibody-1035206_fab105403r">FAB105403R</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-647-conjugated-antibody-1035206_fab105403r" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403N" data-titleoverwrite="Alexa Fluor 700"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-700-conjugated-antibody-1035206_fab105403n">Alexa Fluor 700</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-700-conjugated-antibody-1035206_fab105403n">FAB105403N</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-700-conjugated-antibody-1035206_fab105403n" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403S" data-titleoverwrite="Alexa Fluor 750"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-750-conjugated-antibody-1035206_fab105403s">Alexa Fluor 750</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-750-conjugated-antibody-1035206_fab105403s">FAB105403S</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-750-conjugated-antibody-1035206_fab105403s" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403A" data-titleoverwrite="Allophycocyanin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403a">Allophycocyanin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403a">FAB105403A</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403a" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403APCCY7" data-titleoverwrite="Allophycocyanin/Cy7"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403apccy7">Allophycocyanin/Cy7</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403apccy7">FAB105403APCCY7</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403apccy7" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403B" data-titleoverwrite="Biotin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403b">Biotin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403b">FAB105403B</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403b" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403CL1" data-titleoverwrite="CoraFluor 1"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403cl1">CoraFluor 1</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403cl1">FAB105403CL1</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403cl1" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403D" data-titleoverwrite="DyLight 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403d">DyLight 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403d">FAB105403D</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403d" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403E" data-titleoverwrite="DyLight 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a 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href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403l" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403M" data-titleoverwrite="DyLight 594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403m">DyLight 594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403m">FAB105403M</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403m" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div 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class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403y">DyLight 680</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403y">FAB105403Y</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403y" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403Z" data-titleoverwrite="DyLight 755"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403z">DyLight 755</a> </div> <div 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<a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403f" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403H" data-titleoverwrite="HRP"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403h">HRP</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403h">FAB105403H</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403h" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div 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mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403i">Janelia Fluor 549</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403i">FAB105403I</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403i" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403JF585" data-titleoverwrite="Janelia Fluor 585"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a 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View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403MFV450" data-titleoverwrite="mFluor Violet 450 SE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403mfv450">mFluor Violet 450 SE</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403mfv450">FAB105403MFV450</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403mfv450" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403MFV500" data-titleoverwrite="mFluor Violet 500 SE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403mfv500">mFluor Violet 500 SE</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403mfv500">FAB105403MFV500</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035206_fab105403mfv500" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105403MFV610" data-titleoverwrite="mFluor Violet 610 SE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum 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class="col-lg-9 product-data-value py-2 align-top text-break"> <div class="d-block pb-2"> Flow Cytometry </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Label</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm) </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Antibody Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal Mouse IgG<sub>1</sub> Clone # 1035206 </div> </div> </div> <div class="container"> <div class="row product-data"> <div class="col-lg-6 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4><a href="/t/spike-s1-subunit/antibodies">View all Spike S1 Subunit Antibodies »</a></h4> </div> </div> </div> </div> <div class="border-top border-bottom recommended-products-section" id="recommendation-viewed" data-product_code="FAB105403U" data-type=viewed></div> <div id="product-specifications" class="bt-pp-border-bottom py-5" data-section="section-base--product-specifications"> <h3>Product Specifications</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Immunogen</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> HEK293-derived SARS-CoV-2 Spike S1 Subunit protein<br />Accession # YP_009724390.1 </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Specificity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Detects SARS-CoV-2 Spike S1 subunit in direct ELISAs and Western blots. No cross-reactivity with SARS-CoV-2 Spike RBD is observed in direct ELISAs or Western blots. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Clonality</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Host</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Mouse </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Isotype</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> IgG<sub>1</sub> </div> </div> </div> </div> <div id="applications" class="py-5 bt-pp-border-bottom" data-anchor-label="Applications" data-section="section-antibody--applications"> <h3>Applications </h3> <div class="mt-5 py-2 container"> <div class="row"> <div class="col-lg-3 border font-weight-bold text-bg-dark py-2 align-top text-md-left text-white bg-dark-bt-blue d-none d-lg-block"> Application </div> <div class="col-lg-9 border align-top py-2 font-weight-bold text-white bg-dark-bt-blue d-none d-lg-block"> Recommended Usage </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Flow Cytometry</h4> </div> <div class="col-lg-9 border align-top py-2"> 0.25-1 µg/10<sup>6</sup> cells <br/>Sample: SARS-CoV-2 Spike protein bound to ACE-2 in live or fixed HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP </div> </div> </div> <div class="product-data-wrapper mt-5"> Please Note: Optimal dilutions of this antibody should be experimentally determined. </div> </div> <div id="preparation--storage" class="bt-pp-border-bottom py-5" data-section="section-base--preparation-storage"> <h3>Formulation, Preparation, and Storage</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Purification</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Protein A or G purified from hybridoma culture supernatant </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Formulation</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Shipping</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Stability & Storage</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <b>Protect from light. </b><b>Do not freeze.</b><UL><LI>12 months from date of receipt, 2 to 8 °C as supplied.</LI></UL> </div> </div> </div> </div> <div id="background" class="py-5 bt-pp-border-bottom" data-section="section-base--background-info"> <h3>Background: Spike S1 Subunit</h3> <article class="product-data-wrapper"> SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). Based on structural biology studies, the receptor binding domain (RBD), located in the C-terminal region of S1, can be oriented either in the up/standing or down/lying state (6). The standing state is associated with higher pathogenicity and both SARS-CoV-1 and MERS can access this state due to the flexibility in their respective RBDs. A similar two-state structure and flexibility is found in the SARS-CoV-2 RBD (7). Based on amino acid (aa) sequence homology, the SARS-CoV-2  S1 subunit has 65% identity with SARS-CoV-1 S1 subunit,  but only 22% homology with the MERS S1 subunit. The low aa sequence homology is consistent with the finding that SARS and MERS bind different cellular receptors (8). The S Protein of the SARS-CoV-2 virus, like the SARS-CoV-1 counterpart, binds Angiotensin-Converting Enzyme 2 (ACE2), but with much higher affinity and faster binding kinetics (9). Before binding to the ACE2 receptor, structural analysis of the S1 trimer shows that only one of the three RBD domains in the trimeric structure is in the "up" conformation. This is an unstable and transient state that passes between trimeric subunits but is nevertheless an exposed state to be targeted for neutralizing antibody therapy (10). Polyclonal antibodies to the RBD of the SARS-CoV-2 S1 subunit have been shown to inhibit interaction with the ACE2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy (11). There is also promising work showing that the RBD may be used to detect presence of neutralizing antibodies present in a patient's bloodstream, consistent with developed immunity after exposure to the SARS-CoV-2 virus (12). Lastly, it has been demonstrated the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (13, 14). </article> <div id="background_wrapper" class="px-3 mt-2"> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>References</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <ol><li>Wu, F. <em>et al</em>. (2020) Nature <strong>579</strong>:265.</li><li>Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. <strong>105</strong>:93.</li><li>Bosch, B.J. <em>et al</em>. (2003) J. Virol. <strong>77</strong>:8801.</li><li>Belouzard, S. <em>et al</em>. (2009) Proc. Natl. Acad. Sci. <strong>106</strong>:5871.</li><li>Millet, J.K. and G. R. Whittaker (2015) Virus Res. <strong>202</strong>:120.</li><li>Yuan, Y. <em>et al</em>. (2017) Nat. Commun. <strong>8</strong>:15092.</li><li>Walls, A.C. <em>et al</em>. (2010) Cell <strong>180</strong>:281.</li><li>Jiang, S. <em>et al</em>. (2020) Trends. Immunol. <a href="https://doi.org/10.1016/j.it.2020.03.007">https://doi.org/10.1016/j.it.2020.03.007</a>.</li><li>Ortega, J.T. <em>et al</em>. (2020) EXCLI J. <strong>19</strong>:410.</li><li>Wrapp, D. <em>et al</em>. (2020) Science <strong>367</strong>:1260.</li><li>Tai, W. <em>et al</em>. (2020) Cell. Mol. Immunol. <a href="https://doi.org/10.1016/j.it.2020.03.007">https://doi.org/10.1016/j.it.2020.03.007</a>.</li><li>Okba, N. M. A. <em>et al</em>. (2020). Emerg. Infect. Dis. <a href="https://doi.org/10.3201/eid2607.200841">https://doi.org/10.3201/eid2607.200841</a>. </li><li>Wang, X. <em>et al</em>. (2020) <a href="https://doi.org/10.1038/s41423-020-0424-9">https://doi.org/10.1038/s41423-020-0424-9</a>.</li><li>Wang, K. <em>et al</em>. (2020) bioRxiv <a href="https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1">https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1</a>. </li></ol> </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Long Name</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> Spike Protein, S1 Subunit </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Alternate Names</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> SARS-CoV-2 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>UniProt</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.uniprot.org/uniprot/?query=YP_009724390.1" class="a___link-external" target="_blank" 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id="specific-notices" class="py-5" data-section="section-base--specific-notices"> <h3>Product Specific Notices </h3> <p><br>This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. 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SARS-CoV-2 Spike S1 Subunit Alexa Fluor® 350-conjugated Antibody (FAB105403U) by R&D Systems, Part of Bio-Techne