Human CCL26/Eotaxin-3 Antibody AF653: R&D Systems

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class="ds_title_table"> <h1 class="ds_title">Human CCL26/Eotaxin-3 Antibody </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: AF653</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 5 Citations </a> </span> <a href="/reviews/AF653" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/human-ccl26-eotaxin-3-antibody_af653" method="post" id="atc-datasheet-form" accept-charset="UTF-8"><div><div id="atc_datasheet_block">     <table class="add_to_cart"> <thead> <tr> <th class="ds_catalog">Catalog #</th> <th class="ds_availability_column mobile-hide">Availability</th> <th class="ds_size_price_column">Size / Price</th> <th class="ds_quantity_column">Qty</th> </tr> </thead> <tbody> <tr class="et_atc_product" data-sku="AF653"> <td class="dat_catalog_column">AF653</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity ds_quantity_column"><div class="form-item form-item-catnum-af653 form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-af653" name="catnum-AF653" value="" size="5" maxlength="3" /></div></td> </tr> <tr class="et_atc_product" data-sku="AF653-SP"> <td class="dat_catalog_column">AF653-SP</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity ds_quantity_column"><div class="form-item form-item-catnum-af653-sp form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-af653-sp" name="catnum-AF653-SP" value="" size="5" maxlength="3" /></div></td> </tr> </tbody> </table> <div class="ds_atc_pre_buttons"></div> <div class="ds_atc_buttons"> <a href="/quote/instruments/ella-automated-immunoassay-system" class="btn btn-secondary atc_request_quote_simple_plex_assay" style="display:none" rel="nofollow">Request a Quote</a> <a href="/services/bulk-quote-request?catnum=AF653&product=Human CCL26/Eotaxin-3 Antibody" class="btn btn-secondary atc_hidden bulk_btn" style="display:none;" rel="nofollow" title="Competitive discounts on products purchased in bulk quantities">Bulk Orders</a> <button class="btn btn-primary atc_hidden atc_btn form-submit icon-before" style="display:none;" title="Enter quantities above and click to add to cart" type="submit" id="edit-add-to-cart" name="add_to_cart_btn" value="Add to Cart"><span class="icon glyphicon glyphicon-plus" aria-hidden="true"></span> Add to Cart</button> </div> <div class="ds_atc_post_buttons"></div> </div> <input type="hidden" name="form_build_id" value="form-vxMXVmmO-hARtAINpIJ4KzEuTxAb67yHOJV_GV6DzjU" /> <input type="hidden" name="form_id" value="atc_datasheet_form" /> </div></form> </div> </div> <div class="product_image_wrapper col-xs-12 col-md-4 col-md-pull-8"> <div class="product_image"> <span class="product_image_label"> </span> <a class="ds-image overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="Detection of Human CCL26/Eotaxin‑3 by Western Blot." data-footer="Detection of Human CCL26/Eotaxin‑3 by Western Blot." title="Cat. No. AF653 - Human CCL26/Eotaxin-3 Antibody" href="https://resources.rndsystems.com/images/datasheets/antibody/CCL26_AF653_Western_Blot_20396.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/antibody/CCL26_AF653_Western_Blot_20396.jpg" class="img-fluid" alt="Detection of Human CCL26/Eotaxin‑3 by Western Blot." title="Detection of Human CCL26/Eotaxin‑3 by Western Blot."> </a> <div class="search_image_other_count"> <span class="search_more_images">3 Images</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="CCL26/Eotaxin‑3 in Human Colon (Vasculature)." data-title="CCL26/Eotaxin‑3 in Human Colon (Vasculature)." title="Cat. No. AF653 - Human CCL26/Eotaxin-3 Antibody" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/antibody/af653_human-ccl26-eotaxin-3-affinity-purified-polyclonal-ab-immunohistochemistry-3092021152219.jpg"> <img src="https://resources.rndsystems.com/images/datasheets/antibody/af653_human-ccl26-eotaxin-3-affinity-purified-polyclonal-ab-immunohistochemistry-3092021152219.jpg" class="img-fluid" loading="lazy" alt="CCL26/Eotaxin‑3 in Human Colon (Vasculature)." title="CCL26/Eotaxin‑3 in Human Colon (Vasculature)."> </a> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody." data-title="Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody." title="Cat. No. AF653 - Human CCL26/Eotaxin-3 Antibody" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/antibody/CCL26_AF653_Block_Neutralize_8939.jpg"> <img src="https://resources.rndsystems.com/images/datasheets/antibody/CCL26_AF653_Block_Neutralize_8939.jpg" class="img-fluid" loading="lazy" alt="Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody." title="Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody."> </a> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/ccl26-eotaxin-3?category=Primary Antibodies" class="btn btn-secondary">All CCL26/Eotaxin-3 Antibodies</a> </div> </div> <div class="tabbed_content" id="rnd_antibody"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (5)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_calculator" id="product-anchor-ds_calculator">Reconstitution Calculator</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <h2 id="ds_summary">Human CCL26/Eotaxin-3 Antibody Summary</h2> <div class="datasheet_section"> <div class="ds_table summary_table"> <div class="ds_table_body">  <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Species Reactivity</div> <div class="ds_table_cell summary_table_data"> Human </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data"> Detects CCL26/Eotaxin‑3 in direct ELISAs and Western blots. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Source</div> <div class="ds_table_cell summary_table_data"> Polyclonal Goat IgG </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Purification</div> <div class="ds_table_cell summary_table_data"> Antigen Affinity-purified </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Immunogen</div> <div class="ds_table_cell summary_table_data"> <EM>E. coli</EM>-derived recombinant human CCL26/Eotaxin‑3 <br />Thr24-Leu94<br />Accession # Q9Y258 </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Formulation</div> <div class="ds_table_cell summary_table_data"> Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Endotoxin Level</div> <div class="ds_table_cell summary_table_data"> <0.10 EU per 1 μg of the antibody by the LAL method. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Label</div> <div class="ds_table_cell summary_table_data"> Unconjugated </div> </div> </div> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="AF653"></div> <div id="ds_applications" class="datasheet_section"> <h2>Applications</h2> <div class="ds_table app_table"> <div class="ds_table_head"> <div class="ds_table_head_row"> <div class="ds_table_head_cell app_table_blank"></div> <div class="ds_table_head_cell app_table_rec_conc">Recommended Concentration</div> <div class="ds_table_head_cell app_table_sample">Sample</div> </div> </div> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell app_table_name">Western Blot</div> <div class="ds_table_cell app_table_dilution">1 µg/mL</div> <div class="ds_table_cell app_table_sample">See below</div> </div> <div class="ds_table_row"> <div class="ds_table_cell app_table_name ich_cell">Immunohistochemistry</div> <div class="ds_table_cell app_table_dilution">5-15 µg/mL</div> <div class="ds_table_cell app_table_sample">Immersion fixed paraffin-embedded sections of human colon (vasculature)</div> </div> </div> </div> <div class="ds_table summary_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Neutralization</div> <div class="ds_table_cell summary_table_data">Measured by its ability to neutralize CCL26/Eotaxin‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND<sub>50</sub>) is typically 4-24 µg/mL in the presence of 1 µg/mL Recombinant Human CCL26/Eotaxin‑3. </div> </div> </div> </div> <p>Please Note: Optimal dilutions should be determined by each laboratory for each application. <a href="/resources/protocols-troubleshooting-guides">General Protocols</a> are available in the Technical Information section on our website. </p> </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> Western Blot </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="Detection of Human CCL26/Eotaxin‑3 by Western Blot." data-footer="Detection of Human CCL26/Eotaxin-3 antibody by Western Blot." title="Cat. No. AF653 - Detection of Human CCL26/Eotaxin-3 antibody by Western Blot." href="https://resources.rndsystems.com/images/datasheets/antibody/CCL26_AF653_Western_Blot_20396.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/antibody/CCL26_AF653_Western_Blot_20396.jpg" class="img-fluid" alt="Detection of Human CCL26/Eotaxin-3 antibody by Western Blot." title="Detection of Human CCL26/Eotaxin-3 antibody by Western Blot."> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Detection of Human CCL26/Eotaxin‑3 by Western Blot.</span> Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL-4 (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/recombinant-human-il-4-protein_204-il">204-IL</a>) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL26/Eotaxin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF653) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/goat-igg-hrp-conjugated-antibody_haf017">HAF017</a>). A specific band was detected for CCL26/ Eotaxin-3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using <a class="NoLineLink" href="https://www.rndsystems.com/go/westernblotconditions">Immunoblot Buffer Group 1</a>. </p> </div> </div> <div class="row data_ex_row"> <a id="ds_image_1" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> Immunohistochemistry </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-1" data-title="CCL26/Eotaxin‑3 in Human Colon (Vasculature)." data-footer="" title="Cat. No. AF653 - " href="https://resources.rndsystems.com/images/datasheets/antibody/af653_human-ccl26-eotaxin-3-affinity-purified-polyclonal-ab-immunohistochemistry-3092021152219.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/antibody/af653_human-ccl26-eotaxin-3-affinity-purified-polyclonal-ab-immunohistochemistry-3092021152219.jpg" class="img-fluid" alt="" title=""> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">CCL26/Eotaxin‑3 in Human Colon (Vasculature).</span> CCL26/Eotaxin‑3 was detected in immersion fixed paraffin-embedded sections of human colon (vasculature) using Goat Anti-Human CCL26/Eotaxin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF653) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class="NoLineLink" href="https://www.rndsystems.com/products/goat-igg-visucyte-hrp-polymer_vc004">VC004</a>). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (<a class="NoLineLink" href="https://www.rndsystems.com/products/antigen-retrieval-reagent-basic_cts013">CTS013</a>). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on endothelial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. </p> </div> </div> <div class="row data_ex_row"> <a id="ds_image_2" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> Neutralization </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-2" data-title="Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody." data-footer="Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody." title="Cat. No. AF653 - Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody." href="https://resources.rndsystems.com/images/datasheets/antibody/CCL26_AF653_Block_Neutralize_8939.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/antibody/CCL26_AF653_Block_Neutralize_8939.jpg" class="img-fluid" alt="Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody." title="Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody."> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio by Human CCL26/Eotaxin‑3 Antibody.</span> Recombinant Human CCL26/Eotaxin-3 (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/recombinant-human-ccl26-eotaxin-3-aa-24-94-protein_346-e3">346-E3</a>) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/resazurin_ar002">AR002</a>). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF653). The ND<SUB>50</SUB> is typically 4-24 µg/mL. </p> </div> </div> </div> </div> </div> <div id="ds_calculator" class="datasheet_section"> <div id="btn_calculator_wrapper"><div class="datasheet_calculator_toggle btn btn-secondary" id="btn_reconstitution">Reconstitution Calculator</div></div><div id="datasheet_calculators"><div id="btn_reconstitution_calc" class="datasheet_calculator"><h2>Reconstitution Calculator</h2><div id="reconstitution_calculator" class="calculator"> <form id="recCalc"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume (to add to vial)</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </span> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass (in vial)</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </span> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Desired Reconstitution Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="conc." class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </span> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button btn btn-secondary"/> </div> </form> </div> </div></div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Reconstitution</div> <div class="ds_table_cell prep_table_data">Reconstitute at 0.2 mg/mL in sterile PBS. <div id="reconstitution-buffer"> <div id="reconstitution-buffer-placeholder"> <div class="et_spinner_small"></div> Loading... </div> </div> </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Stability & Storage</div> <div class="ds_table_cell prep_table_data"><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL> </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: CCL26/Eotaxin-3</h2> <p><P>Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin‑3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4.</P> <P>Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. Recombinant Eotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in <EM>E. coli</EM> and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.</P></p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name"><a id="reftop"></a>References</div> <div class="ds_table_cell background_table_data"><OL> <LI>Gou, R.F. <EM>et al</EM>. (1999) Genomics <STRONG>58</STRONG>:313.</LI> <LI>Kitamura, M. <EM>et al</EM>. (1999) J. Biol. Chem. <STRONG>274</STRONG>:27975.</LI> <LI>Shinkai, A. <EM>et al</EM>. (1999) J. Immunol. <STRONG>163</STRONG>:1602.</LI></OL></div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs</div> <div class="ds_table_cell background_table_data">10344 (Human)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names</div> <div class="ds_table_cell background_table_data">CC chemokine IMAC; C-C motif chemokine 26; CCL26; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin3; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4a; MIP-4alpha; SCYA26; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; TSC-1Thymic stroma chemokine-1</div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/af653.pdf?v=20241123" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-ccl26-eotaxin-3-antibody_af653">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//aero.bio-techne.com/sds/pdf/af653?v=20241123">SDS</a> </div> </div> </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/cc-chemokines-and-receptors">CC Chemokines and Receptors</a> </li> <li> <a href="/research-area/chemokines-and-receptors">Chemokines and Receptors</a> </li> <li> <a href="/research-area/chemokines-and-receptors-in-allergy-and-asthma">Chemokines and Receptors in Allergy and Asthma</a> </li> <li> <a href="/research-area/macrophage-activation-markers">Macrophage Activation Markers</a> </li> </ul> </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for Human CCL26/Eotaxin-3 Antibody</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">5</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">5</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Human"> Human </option> </select> </div> <div class="select_wrapper filter_select"> <select id="selAntibody" title="Applications"> <option value="All">All Applications</option> <option class="citation_filterRadio" value="application" data-filter="IHC"> IHC </option> <option class="citation_filterRadio" value="application" data-filter="IHC-Fr"> IHC-Fr </option> <option class="citation_filterRadio" value="application" data-filter="IHC-P"> IHC-P </option> </select> </div> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="Whole Tissue"> Whole Tissue </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="5" data-max-show="10"> <li class="top-lined" data-species="Human" data-application="IHC-Fr" data-sampletype="Whole Tissue"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/28648867"> An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. </a> </span><br/> Authors: Rump L, Mattey D, Kehoe O, Middleton J<br/> Cytokine, 2017-09-01;97(0):133-140.<br/> Species: Human<br/> Sample Types: Whole Tissue<br/> Applications: IHC-Fr </li> <li class="top-lined" data-species="" data-application="" data-sampletype=""> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/25963082"> Clinicopathologic Analysis of Proton Pump Inhibitor-Responsive Esophageal Eosinophilia in Korean Patients </a> </span><br/> Authors: Da Hyun Jung, Gak-Won Yun, Yoo Jin Lee, Yunju Jo, Hyojin Park<br/> Gut and Liver<br/> </li> <li class="top-lined" data-species="Human" data-application="IHC" data-sampletype="Whole Tissue"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/24988451"> Proton pump inhibitors decrease eotaxin-3 expression in the proximal esophagus of children with esophageal eosinophilia. </a> </span><br/> Authors: Park, Jason Y, Zhang, Xi, Nguyen, Nathalie, Souza, Rhonda F, Spechler, Stuart J, Cheng, Edaire<br/> PLoS ONE, 2014-07-02;9(7):e101391.<br/> Species: Human<br/> Sample Types: Whole Tissue<br/> Applications: IHC </li> <li class="top-lined" data-species="Human" data-application="IHC-P" data-sampletype="Whole Tissue"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/21881593"> High Levels of CCL26 in Blister Fluid and Sera of Patients with Bullous Pemphigoid. </a> </span><br/> Authors: Kagami S, Kai H, Kakinuma T<br/> J. Invest. Dermatol., 2011-09-01;132(1):249-51.<br/> Species: Human<br/> Sample Types: Whole Tissue<br/> Applications: IHC-P </li> <li class="top-lined" data-species="" data-application="" data-sampletype=""> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/34762602"> 15LO1 dictates glutathione redox changes in asthmatic airway epithelium to worsen type-2 inflammation </a> </span><br/> Authors: Nagasaki T, Schuyler AJ, Zhao J Et al.<br/> The Journal of clinical investigation<br/> </li> </ol> </div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/primary-antibodies"> View all Antibody FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="af653" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Human CCL26/Eotaxin-3 Antibody</h3> <div class="row"> <div class="col-xs-12"> <div> <div class="row"> <div class="col-md-6"> <p>There are currently no reviews for this product. 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Human CCL26/Eotaxin-3 Antibody AF653: R&D Systems