ELISA calculator

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id="species-reactivity-Mouse"> <label class="form-check-label" for="species-reactivity-Mouse"> Mouse </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Rat" id="species-reactivity-Rat"> <label class="form-check-label" for="species-reactivity-Rat"> Rat </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Pig" id="species-reactivity-Pig"> <label class="form-check-label" for="species-reactivity-Pig"> Pig </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Bovine" id="species-reactivity-Bovine"> <label class="form-check-label" for="species-reactivity-Bovine"> Bovine </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Chicken" id="species-reactivity-Chicken"> <label class="form-check-label" for="species-reactivity-Chicken"> Chicken </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Zebrafish" id="species-reactivity-Zebrafish"> <label class="form-check-label" for="species-reactivity-Zebrafish"> Zebrafish </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Hamster" id="species-reactivity-Hamster"> <label class="form-check-label" for="species-reactivity-Hamster"> Hamster </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Monkey" id="species-reactivity-Monkey"> <label class="form-check-label" 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class="search-term-layout__term">Conjugate</div> <div class="search-term-layout__count">0</div> </div> </div> <div class="search__dropdown"> <ul class="list-unstyled m-0"> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Alexa Fluor® 488" id="conjugate-alexa-fluor-488"> <label class="form-check-label" for="conjugate-Alexa-Fluor-488"> Alexa Fluor® 488 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Alexa Fluor® 568" id="conjugate-alexa-fluor-568"> <label class="form-check-label" for="conjugate-Alexa-Fluor-568"> Alexa Fluor® 568 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Alexa Fluor® 647" id="conjugate-alexa-fluor-647"> <label class="form-check-label" for="conjugate-Alexa-Fluor-647"> Alexa 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id="conjugate-CoraLite-594"> <label class="form-check-label" for="conjugate-CoraLite-594"> CoraLite®594 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®647" id="conjugate-CoraLite-647"> <label class="form-check-label" for="conjugate-CoraLite-647"> CoraLite®647 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="cy3" id="conjugate-cy3"> <label class="form-check-label" for="conjugate-Cy3"> Cy3 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="FITC" id="conjugate-FITC"> <label class="form-check-label" for="conjugate-FITC"> FITC </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="HRP" id="conjugate-HRP"> <label class="form-check-label" for="conjugate-HRP"> HRP </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Rhodamine" id="conjugate-rhodamine"> <label class="form-check-label" for="conjugate-Rhodamine"> Rhodamine </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="R-PE" id="conjugate-r-pe"> <label class="form-check-label" for="conjugate-R-PE"> R-PE </label> </div> </li> </ul> <div class="search__dropdown__actions"> <button type="button" class="btn btn-sm btn-text search__dropdown__actions__clear">Clear</button> <button type="button" class="btn btn-sm btn-primary search__dropdown__actions__confirm">Confirm</button> </div> </div> </div> <div class="search__params" tabindex="0" data-search-field="compatible-species"> <div 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data-page="generic_page"> <div class="container-xl"> <div class="row justify-content-center"> <div class="col-12"> <div class="basket-progress title-block"> <div class="basket-progress-inner" style="width:100%;"> <ul class="nav" role="tablist"> <li class="current stepItem" role="presentation"> <a id="First-Step-tab" role="tab" data-bs-toggle="tab" data-bs-target="#First-Step" aria-controls="First-Step" aria-selected="true" class="stepa">Data import and value assignment</a> </li> <li class="prev stepItem" role="presentation"> <a id="Second-Step-tab" role="tab" data-bs-toggle="tab" data-bs-target="#Second-Step" aria-controls="Second-Step" aria-selected="false" class="stepa">Curve Fit</a> </li> <li class="prev stepItem" role="presentation"> <a id="Third-Step-tab" role="tab" data-bs-toggle="tab" data-bs-target="#Third-Step" aria-controls="Third-Step" aria-selected="false" class="stepa">Concentration calculation</a> </li> </ul> </div> </div> </div> <div class="tab-content"> <div id="First-Step" class="tab-pane fade show active" role="tabpanel" aria-labelledby="First-Step-tab"> <div class="p-3"> <div class="row"> <div id="frmDataAnalysis"> <div id="frmImportData" class="row">  <div class="col-12 col-lg-6" id="raw_data"> <div class="row"> <div class="col-12 col-lg-5"> <span class="stepTitle">Data Importing</span> <img name="DataImporting" class="tip-icon" src="/img/icons/icon_tip.png" /> </div> <div class="col-12 col-lg-7"> <div name="DataImporting" class="tipBox toolTipBox"> <div class="tipContent"> Enter or paste the raw OD measurement data in a 12x8 matrix, leaving a space between each value as shown in the example data (you can easily overwrite this when inputting your own) </div> </div> </div> </div> <div class="row"> <span class="titlesize"> Paste your unedited OD data from a Word or Excel table into the text box below (Numeric format only) </span> </div> <div class="row"> <textarea id="input_OD_entry" name="input_OD_entry" type="text" rows="10" cols="100" style="overflow:scroll !important; white-space:nowrap"></textarea> </div> </div>  <div class="col-12 col-lg-6" id="Conc_type"> <div class="row"> <div class="col-12 col-lg-12"> <span class="stepTitle">Standard Concentrations Settings</span> <img name="Standard" class="tip-icon" src="/img/icons/icon_tip.png" /> <div style="position:absolute;top:230px;width:600px;"> <div name="Standard" class="bottombox toolTipBox"> <div class="tipContent"> For your standard concentration, enter the first (s1) or last (s6) standard concentration and select x2 or ÷2 depending on where you want the series calculations to start. A table with the standard series values will appear below this box once you click submit, check this is correct. </div> </div> </div> </div> </div> <div class="row" id="Conc_setting"> <div class="row" id="Standard_Type"> <div class="col-4"> <input id="chk6S" name="chkType" value="1" type="radio" checked /> <span>7 Standards</span> </div> <div class="col-4"> <input id="chk11S" name="chkType" value="2" type="radio" /> <span>11 Standards</span> </div> <div class="col-4"> <input id="chkCustom" name="chkType" value="3" type="radio" /> <span>Custom Standards</span> </div> </div> <div class="row" id="standard-Concentrations-Settings-Custom" style="display:none"> <div class="row"> <div class="col-12 col-lg-4 mt-lg-0"> <span class="titlesize">Sample Gradient Direction</span> <select id="sltSampleGradientDirection"> <option value="1">→</option> <option value="2">↓</option> </select> </div> <div class="col-3 col-lg-2 mt-lg-0" title="Numeric format only!"> <span class="titlesize">Standards</span> <input id="txtStandards" type="number" min="0" /> </div> <div class="col-3 col-lg-3 mt-lg-0" title="Numeric format only!"> <span class="titlesize" style="font-size: 12px;">Standards Replications</span> <input id="txtReplications" type="number" min="0" /> </div> <div class="col-6 col-lg-3 mt-lg-0" title="Numeric format only!"> <span class="titlesize">Sample Replications</span> <input id="samReplications" type="number" min="0" /> </div> <div class="col-12 col-lg-12" style=" justify-content: space-between; align-content: space-around; flex-wrap: wrap;"> <input id="chkBlank" name="chkType" value="1" type="checkbox"> Blank <input id="chkControl" name="chkType" value="2" type="checkbox"> <span class="titlesize">Control</span> <input id="chkPlace" name="chkType" value="3" type="checkbox"> <span class="titlesize">First place?</span> </div> </div> </div> <div class="row" id="standard-Concentrations-Settings-Normal"> <div class="col-12 col-lg-7" title="Set your initial concentration!"> <div class="row"> <span class="titlesize">Initial standard concentration</span> <div class="col-4"> <select id="initConloaction"> <option value="0">s1</option> </select> </div> <div class="col-8"> <input id="txtInitCon" class="col-3" type="text" placeholder="Set your initial concentration" /> </div> </div> </div> <div class="col-12 col-lg-3"> <span class="titlesize">Change Of Gradient</span> <select id="sltGradientOperator"> <option value="0">➗</option> <option value="1">✖</option> </select> </div> <div class="col-12 col-lg-2 mt-3"> <select id="sltGradientValue" style="font-size:20px;"> <option value="0">2</option> <option value="1">3</option> <option value="2">4</option> </select> </div> </div> <div class="row"> <div class="col-12 col-lg-4" id="normal_samselect"> <span class="input-group-addon">Sample Replications:</span> <select id="normal_samrepli"> <option value="1">1</option> <option value="2">2</option> <option value="3">3</option> </select> </div> </div> <div class="row"> <div class="col-12 col-lg-12"> <div class="col-3 col-lg-3 float-end ms-2"> <button id="resetAnalysisData" type="button" class="small bg-ptg-grey-4"> Clear </button> </div> <div class="col-3 col-lg-3 float-end"> <button id="submitAnalysisData" type="button" class="small btn-primary"> Submit </button> </div> </div> </div> </div> </div> </div> <div class="row mt-2" id="Well_Ass"> <div class="row"> <div class="col-12 col-lg-4"> <span class="stepTitle">Well Assignment</span> <img name="WellAssignment" class="tip-icon" src="/img/icons/icon_tip.png" /> </div> <div class="col-12 col-lg-5"> <div name="WellAssignment" class="tipBox toolTipBox" style="top: 0; left: -30%;"> <div class="tipContent"> Ensure the “Well assignment” is laid out in the same way as the standards and samples of your experiment. You can use our suggested templates as for 7 standards or 11 standards or customise the layout with options to include blanks and controls. </div> </div> </div> </div> <div class="col-12 col-lg-6" id="seats-container"> </div> <div class="col-12 col-lg-2" id="seats-legend"> </div> <div class="col-12 col-lg-3" id="Conc_table"> </div> </div> </div> </div> </div> </div> <div id="Second-Step" class="tab-pane fade" role="tabpanel" aria-labelledby="Second-Step-tab"> <div class="p-3"> <div class="row"> <div id="frmCurveFit" class="row"> <div class="row mt-1"> <div class="col-12 col-lg-4"> <span>Regression /Fitting model</span> <img name="RegressionFitting" class="tip-icon" src="/img/icons/icon_tip.png" style="margin-bottom:0px;" /> <div style="position:absolute;top:220px;width:600px;"> <div name="RegressionFitting" class="bottomboxstep2 toolTipBox"> <div class="tipContent"> Choose which regression model best fits your data. The standard concentrations and OD values will be automatically included from the previous tab. Our recommended model for most ELISA experiments is the four-parameter logistic (4PL) curve fit. </div> </div> </div> <select id="fitType"> <option value="1">Linear Regression</option> <option selected value="2">Four Parameter Logistic (4-PL) Curve Fit</option> <option value="3">Semilogarithmic Fit</option> <option value="4">Log-Log Fit</option>  </select> </div> <div class="col-12 col-lg-4 mt-2 mt-lg-0" title="enter in or paste your concentration data"> <span>Standard concentrations</span> <input id="txtCon" class="col-6" type="text" required placeholder="enter in or paste your concentration data" /> </div> <div class="col-12 col-lg-3" title="enter in or paste your OD data"> <span>Standard OD Values</span> <input id="txtOD" class="col-8" type="text" required placeholder="enter in or paste your OD data" /> </div> </div> <div class="row"> <div class="col-12 col-lg-3"> <span>X data column</span> <img name="CurveFitXColumn" class="tip-icon" src="/img/icons/icon_tip.png" style="margin-bottom: 0px;" /> <div style="position:absolute;top:325px;width:600px;"> <div name="CurveFitXColumn" class="bottomboxstepx toolTipBox"> <div class="tipContent"> Most often, data fit best when plotted as logarithm (base 10). Select what is appropriate for your data set. </div> </div> </div> <select id="xaxisType"> <option value="1">Remain unchanged</option> <option selected value="2">Logarithm (base 10)</option> <option value="3">Logarithm (base 2)</option> </select> </div> <div class="col-12 col-lg-3 mt-2 mt-lg-0"> <span>Y data column</span> <select id="yaxisType"> <option value="1">Remain unchanged</option> <option selected value="2">Logarithm (base 10)</option> <option value="3">Logarithm (base 2)</option> </select> </div> <div class="col-4 col-lg-2 mt-2 mt-lg-0"> <span>Legend Name</span> <input type="text" id="pictName" /> </div> <div class="col-4 col-lg-2 mt-2 mt-lg-0"> <span>X axis Name</span> <input type="text" id="xaxName" /> </div> <div class="col-4 col-lg-2 mt-2 mt-lg-0"> <span>Y axis Name</span> <input type="text" id="yaxName" /> </div> </div> </div> <div class="row mt-2"> <div class="col-12 col-lg-2"> <input id="chkReleaseZero" type="checkbox" name="ReleaseZero" checked /> Deduct background <img name="Deductbackground" class="tip-icon" src="/img/icons/icon_tip.png" /> <div style="position:absolute;top:413px;width:600px;"> <div name="Deductbackground" class="bottomboxstepdb toolTipBox"> <div class="tipContent"> If your plate included blank replicates, tick the “deduct background” box to show the corrected values. </div> </div> </div> </div> <div class="col-12 col-lg-8 mt-lg-4"> <div class="col-4 col-lg-2 float-end"> <button id="clearData" type="button" class="small bg-ptg-grey-4"> Clear </button> </div> <div class="col-4 col-lg-2 float-end me-2"> <button id="submitData" type="button" class="btn-primary small"> Submit </button> </div> <div class="col-1 col-lg-1 float-end me-2"> <img name="CurvefitSubmit" class="tip-icon" src="/img/icons/icon_tip.png" /> </div> <div style="position:absolute;top:415px;width:600px;"> <div name="CurvefitSubmit" class="bottombox toolTipBox"> <div class="tipContent"> Click submit to see the standard curve. You can also click the download button on the top right of the graph to save this to your computer as an image. To use this curve to get results, you can hover your mouse over the curve to see the axis coordinate values </div> </div> </div> </div> </div> <div class="row"> <div class="column col-12 col-lg-7"> <div class="the-widget-block the-common-margin-top"> <div id="curveChart" style="width: 100%;height:400px;" margin: auto> </div> </div> </div> <div class="column col-12 col-lg-5"> <table id="table1" border="1" cellpadding="10" cellspacing="0" align="center" width="100%" hidden> <tr> <th style="width:25%">Formula</th> <td id="equation" style="width:75%"></td> </tr> <tr> <th>a value</th> <td id="Params1"></td> </tr> <tr> <th>b value</th> <td id="Params2"> </td> </tr> <tr> <th>c value</th> <td id="Params3"> </td> </tr> <tr> <th>d value</th> <td id="Params4"> </td> </tr> </table> <table id="table2" border="1" cellpadding="10" cellspacing="0" align="center" width="100%" hidden> <thead> <tr> <th style="width:25%">X</th> <th style="width:25%">Y-reaction value</th> <th style="width:25%">Y-calculated value</th> <th style="width:25%">Y-residual</th> </tr> </thead> <tbody id="tbody1"> </tbody> </table> <h4 id="sum"></h4> </div> </div> </div> </div> </div> <div id="Third-Step" class="tab-pane fade" role="tabpanel" aria-labelledby="Third-Step-tab"> <div class="p-3"> <div class="row"> <div id="frmConCaculator"> <div class="row"> <div class="col-6 col-lg-2"> <button id="btnDataSummary" type="button" class="btn-primary small"> Calculate results </button> </div> <div class="col-1 col-lg-1"> <img name="calculateResults" class="tip-icon" src="/img/icons/icon_tip.png"> </div> <div class="col-12 col-lg-5"> <div name="calculateResults" class="tipBox toolTipBox" style="top: 0; left: -10%;"> <div class="tipContent"> Use the ‘download’ button to export the table. Be mindful to adjust for dilution factors and replicate averages if not accounted for previously. </div> </div> </div> </div> <div class="row" style="margin-bottom:5px"> <div class="col-12 col-lg-7"> <div class="input-group"> <span class="input-group-addon mt-1">Total absorbance value (ODs):</span> <input id="totalOD" type="text" class="ms-1" name="totalods" placeholder="enter in or paste your OD data" readonly /> </div> </div> <div class="col-12 col-lg-5"> <div class="input-group"> <span class="input-group-addon mt-1">Blank Value </span> <input id="blank_val" type="text" class="ms-1" name="bv" /> </div> </div> </div> <div class="row" style="margin-bottom:5px"> <table id="Datasumtable" border="1" cellpadding="10" cellspacing="0" align="center" width="100%" hidden> <thead> <tr> <th>ID</th> <th>Well(s)</th> <th>Raw OD value(s)</th> <th>Replicate Average</th> <th>Infact OData</th> <th>Conc.</th> </tr> </thead> <tbody id="datasum_tbody"> </tbody> </table> </div> </div> </div> </div> </div> <div class="row" style="margin-bottom:5px"> <button id="btnExportExcel" class="btn btn-info" style="display:none;">export to Excel</button> </div> <div class="row" id="SumDataEx" style="width: 80%; margin-left: 200px; margin-top: 100px; visibility: hidden;"> <table id="SumDataExcel"> <thead> <tr><th>This report is generated by Proteintech (Proteintech ELISA Data Analyzer). https://www.ptgcn.com/products/elisa-kits/#ELISAAssayCalc</th></tr> <tr></tr> <tr><th>Experiment name</th></tr> <tr></tr> <tr><th>Standard concentration unit</th></tr> <tr></tr> <tr><th>Formula</th><th colspan='1' id="equa"></th></tr> <tr><th>a value</th><th id="Para1"></th></tr> <tr><th>b value</th><th id="Para2"></th></tr> <tr><th>c value</th><th id="Para3"></th></tr> <tr><th>d value</th><th id="Para4"></th></tr> <tr><th>R^2</th><th id="ParaR2"></th></tr> <tr></tr> <tr><th>ID</th><th>Well(s)</th><th>Raw OD value(s)</th><th>Replicate Average</th><th>Infact OData</th><th>Conc.</th></tr> </thead> <tbody class="datasum_tbody"> </tbody> </table> </div> </div> </div> </div> </section> <div class="scroll-to-top"> <a href="#top" title="Back to Top" class="btn btn-roundel"> <svg xmlns="http://www.w3.org/2000/svg" fill="currentColor" viewBox="0 0 16 16"> <path fill-rule="evenodd" d="M8 15a.5.5 0 0 0 .5-.5V2.707l3.146 3.147a.5.5 0 0 0 .708-.708l-4-4a.5.5 0 0 0-.708 0l-4 4a.5.5 0 1 0 .708.708L7.5 2.707V14.5a.5.5 0 0 0 .5.5z"></path> </svg> </a> </div> <footer class="footer"> <div class="container-xl"> <div class="row d-flex d-lg-none"> <div class="col-12 text-center"> <hr> <ul class="inline-list m-0 p-0 opacity-7"> <li class="list-inline-item"> <a href="http://www.youtube.com/proteintech"> <img loading="lazy" src="/img/logos/Icon_Youtube.svg" width="42"> </a> </li> <li class="list-inline-item"> <a href="https://www.linkedin.com/company/proteintech-group/"> <img loading="lazy" src="/img/logos/Icon_Linkedin.svg" width="42"> </a> </li> <li class="list-inline-item"> <a href="http://www.twitter.com/proteintech"> <img loading="lazy" src="/img/logos/Icon_Twitter.svg" width="42"> </a> </li> <li class="list-inline-item"> <a href="https://www.facebook.com/Proteintech-311515338877884/timeline/"> <img loading="lazy" src="/img/logos/Icon_Facebook.svg" width="42"> </a> </li> <li class="list-inline-item"> <a href="https://www.instagram.com/proteintech/"> <img loading="lazy" src="/img/logos/Icon_Instagram.svg" width="42"> </a> </li> </ul> <hr> <p class="fw-semi m-4"> <a class="mx-2" href="/contact/">Contact Us</a> <a class="mx-2" href="/news/blog/privacy-policy/">Privacy Policy</a> <a class="mx-2" href="/terms-of-use/">Terms of use</a> <a class="mx-2" href="/news/blog/product-terms/">Terms and Conditions</a> <a class="mx-2" href="/trademark-information/">Trademark Information</a> <a class="mx-2" href="/imprint-impressum/" target="_blank">Imprint (Impressum)</a> <a class="mx-2" href="/modern-slavery-statement/">Modern Slavery Statement</a> <a class="mx-2" href="/chromotek-privacy-policy/">ChromoTek GmbH Privacy Policy</a> </p> <p class="fw-semi"> Copyright © 2002-2022 Proteintech Group, Inc. 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