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Search </a> </div> </div> </header> <div class="masthead-none"></div> <div class="breadcrumbs"> <div class="bound-layout"> <nav> <a href="https://asuragen.com">Home</a><a href="https://asuragen.com/portfolio/">Portfolio</a><a href="https://asuragen.com/portfolio/genetics/">Kitted Assays for Inherited Genetic Disorders</a><a>AmplideX® PCR/CE <em>HTT</em> Kit</a> </nav> </div> </div> <div class="bound-layout layout-sidebar"> <div class="content"> <div class="solutions-detail"> <div class="solution-description" id="solution-us"> <h1>AmplideX® PCR/CE <em>HTT</em> Kit</h1> <p><a href="https://asuragen.com/wp-content/uploads/2018/12/HTT-box-image-e1544544515133.jpg" target="_blank" rel="noopener"><img loading="lazy" decoding="async" class="alignleft wp-image-190044" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_358,h_293/https://asuragen.com/wp-content/uploads/2018/12/HTT-box-image-e1544544515133.jpg" alt="" width="358" height="293" /></a></p> <p> </p> <p>The AmplideX PCR/CE <em>HTT </em>Kit* makes the detection and sizing of CAG trinucleotide repeats in the <em>HTT</em> gene easier than ever. In just a single PCR reaction, the assay overcomes the challenges of adjacent SNPs and variable CCG repeats to accurately and reliably report the number of repeats in one day, from as little as 10ng gDNA.</p> <p style="text-align: center;"><a class="btn -large -underline" href="#order" rel="noopener">Order the AmplideX PCR/CE HTT Kit</a></p> <nav class="solutions-nav stacked"> <a href="#us-1">Features & Benefits</a> <a href="#us-2">Analytical Characteristics</a> <a href="#us-3">Order Now</a> <a href="#us-4">Ordering Information</a> <a href="#us-5">More Huntington's Disease Research Products</a> </nav> <div id="us-1" class="solutions-detail-content"> <h3 class="solution-subtitle">Features & Benefits</h3> <p>The AmplideX PCR/CE <em>HTT</em> Kit includes reagents to perform a two-primer, anchor-primed PCR of the <em>HTT</em> CAG repeat region followed by fragment sizing by capillary electrophoresis. This single-tube PCR reaction provides a streamlined workflow, enabling sample-to-result in approximately 6 hours using readily available laboratory equipment.</p> <h5>Reduced Complexity</h5> <ul> <li>Proprietary PCR solutions for GC-rich amplification and detection</li> <li>Eliminates need for multiple PCRs – one result, straightforward analysis</li> <li>Resolves zygosity and detects large expansions</li> </ul> <h5>Optimized Workflow</h5> <ul> <li>Fully kitted, end-to-end solutions that significantly reduce hands-on-time</li> <li>Sample-to-result possible within a single shift</li> <li>Identical PCR and CE conditions as the AmplideX PCR/CE <em>DMPK </em>Kit</li> </ul> <h5>Quality Results</h5> <ul> <li>Reliable, unambiguous results and a robust stutter peak pattern</li> <li>Accurate sizing across the entire CAG repeat range</li> <li>Software converts raw base pair data into number of repeats</li> </ul> <p><a href="http://asuragen.com/wp-content/uploads/2021/03/AmplideX-PCRCE-HTT-Kit-Brochure-RUO.pdf" target="_blank" rel="noopener"><img loading="lazy" decoding="async" class="alignnone wp-image-2980" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_109,h_55/https://asuragen.com/wp-content/uploads/2016/07/Download-Brochure-Button_Blue-300x152.png" alt="Download Brochure" width="109" height="55" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2016/07/Download-Brochure-Button_Blue-300x152.png 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_309/https://asuragen.com/wp-content/uploads/2016/07/Download-Brochure-Button_Blue.png 309w" sizes="auto, (max-width: 109px) 100vw, 109px" /></a></p> <p>*For Research Use Only. Not for use in diagnostic procedures.</p> </div> <div id="us-2" class="solutions-detail-content"> <h3 class="solution-subtitle">Analytical Characteristics</h3> <ul> <li>Excellent Concordance with Previously Characterized Samples (Table 1)</li> <li>Repeat profile is conserved across known SNPs (Figure 1)</li> <li>Clearly resolves zygosity (Figure 2)</li> </ul> <p><strong>Table 1.</strong> Comparison of CAG repeat alleles in commercially available samples, as reported by Kalman et al. (2007)</p> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-190047" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_982,h_943/https://asuragen.com/wp-content/uploads/2018/12/HTT-Comparison.jpg" alt="" width="982" height="943" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_982/https://asuragen.com/wp-content/uploads/2018/12/HTT-Comparison.jpg 982w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2018/12/HTT-Comparison-300x288.jpg 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_768/https://asuragen.com/wp-content/uploads/2018/12/HTT-Comparison-768x737.jpg 768w" sizes="auto, (max-width: 982px) 100vw, 982px" /></p> <p><strong>Figure 1.</strong> Repeat profile is conserved across known SNPs.</p> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-190048" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1826,h_745/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs.jpg" alt="" width="1826" height="745" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1826/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs.jpg 1826w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs-300x122.jpg 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_768/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs-768x313.jpg 768w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1024/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs-1024x418.jpg 1024w" sizes="auto, (max-width: 1826px) 100vw, 1826px" /></p> <p><strong>Figure 2.</strong> Zygosity is clearly resolved using the AmplideX PCR/CE<em> HTT</em> Kit.</p> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-190049" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1726,h_703/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity.jpg" alt="" width="1726" height="703" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1726/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity.jpg 1726w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity-300x122.jpg 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_768/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity-768x313.jpg 768w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1024/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity-1024x417.jpg 1024w" sizes="auto, (max-width: 1726px) 100vw, 1726px" /></p> <p><strong>Figure 3.</strong> Workflow for the AmplideX PCR/CE <em>HTT</em> Kit.</p> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-190050" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1423,h_401/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow.png" alt="" width="1423" height="401" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1423/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow.png 1423w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow-300x85.png 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_768/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow-768x216.png 768w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1024/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow-1024x289.png 1024w" sizes="auto, (max-width: 1423px) 100vw, 1423px" /></p> </div> <div id="us-3" class="solutions-detail-content"> <h3 class="solution-subtitle">Order Now</h3> <p id="order">Contact Us Here To Order</p> <p><script src="https://info.bio-techne.com/js/forms2/js/forms2.min.js"></script></p> <form id="mktoForm_9635"></form> <p><script>MktoForms2.loadForm("//info.bio-techne.com", "584-XSF-168", 9635);</script></p> </div> <div id="us-4" class="solution-ordering"> <h3 class="solution-subtitle">Ordering Information</h3> <table class="order-table"> <thead> <tr> <th>Product Name</th> <th>Number of Reactions</th> <th>Catalog Number</th> </tr> </thead> <tbody> <tr> <td>AmplideX® PCR/CE <em>HTT</em> Kit (RUO)</td> <td>32</td> <td>49657</td> </tr> </tbody> </table> <div class="contact"> <div class="direct"> <p> <strong>T</strong> 1-877-777-1874; 512-681-5200 <br> <strong>F</strong> 512-681-5202 <br> </p> </div> </div> </div> <div id="us-5" class="solutions-detail-content"> <h3 class="solution-subtitle">More Huntington's Disease Research Products</h3> <p>Looking for other Huntington’s Disease research products? <a href="https://www.tocris.com/literature/product-guides/huntingtons-disease" target="_blank" rel="noopener">See more from Bio-Techne</a>.</p> </div> </div> <div class="solution-description" id="solution-non-us"> <h1>AmplideX® PCR/CE <em>HTT</em> Kit</h1> <p><a href="https://asuragen.com/wp-content/uploads/2018/12/HTT-box-image-e1544544515133.jpg" target="_blank" rel="noopener"><img loading="lazy" decoding="async" class="alignleft wp-image-190044" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_358,h_293/https://asuragen.com/wp-content/uploads/2018/12/HTT-box-image-e1544544515133.jpg" alt="" width="358" height="293" /></a></p> <p> </p> <p>The AmplideX PCR/CE <em>HTT </em>Kit* makes the detection and sizing of CAG trinucleotide repeats in the <em>HTT</em> gene easier than ever. In just a single PCR reaction, the assay overcomes the challenges of adjacent SNPs and variable CCG repeats to accurately and reliably report the number of repeats in one day, from as little as 10ng gDNA.</p> <p style="text-align: center;"><a class="btn -large -underline" href="https://asuragen.com/contact/" rel="noopener">Order the AmplideX PCR/CE HTT Kit</a></p> <nav class="solutions-nav"> <a href="#non-us-1">Features & Benefits</a> <a href="#non-us-2">Analytical Characteristics</a> <a href="#non-us-3">Order Now</a> <a href="#non-us-4">Ordering Information</a> <a href="#non-us-5">More Huntington's Disease Research Products</a> </nav> <div id="non-us-1" class="solutions-detail-content"> <h3 class="solution-subtitle">Features & Benefits</h3> <p>The AmplideX PCR/CE <em>HTT</em> Kit includes reagents to perform a two-primer, anchor-primed PCR of the <em>HTT</em> CAG repeat region followed by fragment sizing by capillary electrophoresis. This single-tube PCR reaction provides a streamlined workflow, enabling sample-to-result in approximately 6 hours using readily available laboratory equipment.</p> <h5>Reduced Complexity</h5> <ul> <li>Proprietary PCR solutions for GC-rich amplification and detection</li> <li>Eliminates need for multiple PCRs – one result, straightforward analysis</li> <li>Resolves zygosity and detects large expansions</li> </ul> <h5>Optimized Workflow</h5> <ul> <li>Fully kitted, end-to-end solutions that significantly reduce hands-on-time</li> <li>Sample-to-result possible within a single shift</li> <li>Identical PCR and CE conditions as the AmplideX PCR/CE <em>DMPK </em>Kit</li> </ul> <h5>Quality Results</h5> <ul> <li>Reliable, unambiguous results and a robust stutter peak pattern</li> <li>Accurate sizing across the entire CAG repeat range</li> <li>Software converts raw base pair data into number of repeats</li> </ul> <p><a href="https://www2.asuragen.com/l/223762/2019-01-29/9v1kd"><img loading="lazy" decoding="async" class="alignnone wp-image-2980" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_109,h_55/https://asuragen.com/wp-content/uploads/2016/07/Download-Brochure-Button_Blue-300x152.png" alt="Download Brochure" width="109" height="55" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2016/07/Download-Brochure-Button_Blue-300x152.png 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_309/https://asuragen.com/wp-content/uploads/2016/07/Download-Brochure-Button_Blue.png 309w" sizes="auto, (max-width: 109px) 100vw, 109px" /></a></p> <p>*For Research Use Only. Not for use in diagnostic procedures.</p> </div> <div id="non-us-2" class="solutions-detail-content"> <h3 class="solution-subtitle">Analytical Characteristics</h3> <ul> <li>Excellent Concordance with Previously Characterized Samples (Table 1)</li> <li>Repeat profile is conserved across known SNPs (Figure 1)</li> <li>Clearly resolves zygosity (Figure 2)</li> </ul> <p><strong>Table 1.</strong> Comparison of CAG repeat alleles in commercially available samples, as reported by Kalman et al. (2007)</p> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-190047" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_982,h_943/https://asuragen.com/wp-content/uploads/2018/12/HTT-Comparison.jpg" alt="" width="982" height="943" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_982/https://asuragen.com/wp-content/uploads/2018/12/HTT-Comparison.jpg 982w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2018/12/HTT-Comparison-300x288.jpg 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_768/https://asuragen.com/wp-content/uploads/2018/12/HTT-Comparison-768x737.jpg 768w" sizes="auto, (max-width: 982px) 100vw, 982px" /></p> <p><strong>Figure 1.</strong> Repeat profile is conserved across known SNPs.</p> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-190048" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1826,h_745/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs.jpg" alt="" width="1826" height="745" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1826/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs.jpg 1826w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs-300x122.jpg 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_768/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs-768x313.jpg 768w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1024/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-SNPs-1024x418.jpg 1024w" sizes="auto, (max-width: 1826px) 100vw, 1826px" /></p> <p><strong>Figure 2.</strong> Zygosity is clearly resolved using the AmplideX PCR/CE<em> HTT</em> Kit.</p> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-190049" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1726,h_703/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity.jpg" alt="" width="1726" height="703" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1726/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity.jpg 1726w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity-300x122.jpg 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_768/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity-768x313.jpg 768w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1024/https://asuragen.com/wp-content/uploads/2018/12/HTT-Overcoming-Zygosity-1024x417.jpg 1024w" sizes="auto, (max-width: 1726px) 100vw, 1726px" /></p> <p><strong>Figure 3.</strong> Workflow for the AmplideX PCR/CE <em>HTT</em> Kit.</p> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-190050" src="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1423,h_401/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow.png" alt="" width="1423" height="401" srcset="https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1423/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow.png 1423w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_300/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow-300x85.png 300w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_768/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow-768x216.png 768w, https://sp-ao.shortpixel.ai/client/to_webp,q_glossy,ret_img,w_1024/https://asuragen.com/wp-content/uploads/2018/12/HTT-Workflow-1024x289.png 1024w" sizes="auto, (max-width: 1423px) 100vw, 1423px" /></p> </div> <div id="non-us-3" class="solutions-detail-content"> <h3 class="solution-subtitle">Order Now</h3> <p><a class="btn -icon -underline" href="https://asuragen.com/contact/" rel="noopener">Contact Us To Order</a></p> </div> <div id="non-us-4" class="solution-ordering"> <h3 class="solution-subtitle">Ordering Information</h3> <table class="order-table"> <thead> <tr> <th>Product Name</th> <th>Number of Reactions</th> <th>Catalog Number</th> </tr> </thead> <tbody> <tr> <td>AmplideX® PCR/CE <em>HTT</em> Kit (RUO)</td> <td>32</td> <td>49657</td> </tr> </tbody> </table> <div class="contact"> <div class="direct"> <p> <strong>T</strong> 1-877-777-1874; 512-681-5200 <br> <strong>F</strong> 1-512-681-5202 <br> </p> </div> </div> </div> <div id="non-us-5" class="solutions-detail-content"> <h3 class="solution-subtitle">More Huntington's Disease Research Products</h3> <p>Looking for other Huntington’s Disease research products? <a href="https://www.tocris.com/literature/product-guides/huntingtons-disease">See more from Bio-Techne</a>.”</p> </div> </div> </div> </div> <div class="sidebar"> 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