CINXE.COM

<!DOCTYPE html> <html lang="en" dir="ltr"> <head>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-P63WKM1TM1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-P63WKM1TM1'); </script>  <script type="text/javascript" > (function(m,e,t,r,i,k,a){m[i]=m[i]||function(){(m[i].a=m[i].a||[]).push(arguments)}; m[i].l=1*new Date(); for (var j = 0; j < document.scripts.length; j++) {if (document.scripts[j].src === r) { return; }} k=e.createElement(t),a=e.getElementsByTagName(t)[0],k.async=1,k.src=r,a.parentNode.insertBefore(k,a)}) (window, document, "script", "https://mc.yandex.ru/metrika/tag.js", "ym"); ym(55165297, "init", { clickmap:false, trackLinks:true, accurateTrackBounce:true, webvisor:false }); </script> <noscript><div><img src="https://mc.yandex.ru/watch/55165297" style="position:absolute; left:-9999px;" alt="" /></div></noscript>    <title>Search results for: corneal epithelial cells</title> <meta name="description" content="Search results for: corneal epithelial cells"> <meta name="keywords" content="corneal epithelial cells"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="corneal epithelial cells" name="q" aria-label="Search"> <button class="btn btn-light my-2 my-sm-0" type="submit"><i class="fas fa-search"></i></button> </form> </div> <div class="collapse navbar-collapse mt-1" id="navbarMenu"> <ul class="navbar-nav ml-auto align-items-center" id="mainNavMenu"> <li class="nav-item"> <a class="nav-link" href="https://waset.org/conferences" title="Conferences in 2024/2025/2026">Conferences</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/disciplines" title="Disciplines">Disciplines</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/committees" rel="nofollow">Committees</a> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownPublications" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Publications </a> <div class="dropdown-menu" aria-labelledby="navbarDropdownPublications"> <a class="dropdown-item" href="https://publications.waset.org/abstracts">Abstracts</a> <a class="dropdown-item" href="https://publications.waset.org">Periodicals</a> <a class="dropdown-item" href="https://publications.waset.org/archive">Archive</a> </div> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/page/support" title="Support">Support</a> </li> </ul> </div> </div> </nav> </div> </header> <main> <div class="container mt-4"> <div class="row"> <div class="col-md-9 mx-auto"> <form method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="corneal epithelial cells"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 3297</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: corneal epithelial cells</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3087</span> Immunoliposomes Conjugated with CD133 Antibody for Targeting Melanoma Cancer Stem Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chuan%20Yin">Chuan Yin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cancer stem cells (CSCs) represent a subpopulation of cancer cells that possess the characteristics associated with normal stem cells. CD133 is a phenotype of melanoma CSCs responsible for melanoma metastasis and drug resistance. Although adriamycin (ADR) is commonly used drug in melanoma therapy, but it is ineffective in the treatment of melanoma CSCs. In this study, we constructed CD133 antibody conjugated ADR immunoliposomes (ADR-Lip-CD133) to target CD133+ melanoma CSCs. The results showed that the immunoliposomes possessed a small particle size (~150 nm), high drug encapsulation efficiency (~90%). After 72 hr treatment on the WM266-4 melanoma tumorspheres, the IC50 values of the drug formulated in ADR-Lip-CD133, ADR-Lip (ADR liposomes) and ADR are found to be 24.42, 57.13 and 59.98 ng/ml respectively, suggesting that ADR-Lip-CD133 was more effective than ADR-Lip and ADR. Significantly, ADR-Lip-CD133 could almost completely abolish the tumorigenic ability of WM266-4 tumorspheres in vivo, and showed the best therapeutic effect in WM266-4 melanoma xenograft mice. It is noteworthy that ADR-Lip-CD133 could selectively kill CD133+ melanoma CSCs of WM266-4 cells both in vitro and in vivo. ADR-Lip-CD133 represent a potential approach in targeting and killing CD133+ melanoma CSCs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cancer%20stem%20cells" title="cancer stem cells">cancer stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=melanoma" title=" melanoma"> melanoma</a>, <a href="https://publications.waset.org/abstracts/search?q=immunoliposomes" title=" immunoliposomes"> immunoliposomes</a>, <a href="https://publications.waset.org/abstracts/search?q=CD133" title=" CD133"> CD133</a> </p> <a href="https://publications.waset.org/abstracts/32389/immunoliposomes-conjugated-with-cd133-antibody-for-targeting-melanoma-cancer-stem-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/32389.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">382</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3086</span> An Improved Circulating Tumor Cells Analysis Method for Identifying Tumorous Blood Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Salvador%20Garcia%20Bernal">Salvador Garcia Bernal</a>, <a href="https://publications.waset.org/abstracts/search?q=Chi%20Zheng"> Chi Zheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Keqi%20Zhang"> Keqi Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Lei%20Mao"> Lei Mao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Circulating Tumor Cells (CTC) is used to detect tumoral cell metastases using blood samples of patients with cancer (lung, breast, etc.). Using an immunofluorescent method a three channel image (Red, Green, and Blue) are obtained. These set of images usually overpass the 11 x 30 M pixels in size. An aided tool is designed for imaging cell analysis to segmented and identify the tumorous cell based on the three markers signals. Our Method, it is cell-based (area and cell shape) considering each channel information and extracting and making decisions if it is a valid CTC. The system also gives information about number and size of tumor cells found in the sample. We present results in real-life samples achieving acceptable performance in identifying CTCs in short time. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Circulating%20Tumor%20Cells%20%28CTC%29" title="Circulating Tumor Cells (CTC)">Circulating Tumor Cells (CTC)</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20analysis" title=" cell analysis"> cell analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=immunofluorescent" title=" immunofluorescent"> immunofluorescent</a>, <a href="https://publications.waset.org/abstracts/search?q=medical%20image%20analysis" title=" medical image analysis"> medical image analysis</a> </p> <a href="https://publications.waset.org/abstracts/81401/an-improved-circulating-tumor-cells-analysis-method-for-identifying-tumorous-blood-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/81401.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">214</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3085</span> Co-Culture of Neonate Mouse Spermatogonial Stem Cells with Sertoli Cells: Inductive Role of Melatonin following Transplantation: Adult Azoospermia Mouse Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mehdi%20Abbasi">Mehdi Abbasi</a>, <a href="https://publications.waset.org/abstracts/search?q=Shadan%20Navid"> Shadan Navid</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Pourahmadi"> Mohammad Pourahmadi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Majidi%20Zolbin"> M. Majidi Zolbin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We have recently reported that melatonin as antioxidant enhances the efficacy of colonization of spermatogonial stem cells (SSCs). Melatonin as an antioxidant plays a vital role in the development of SSCs in vitro. This study aimed to investigate evaluation of sertoli cells and melatonin simultaneously on SSC proliferation following transplantation to testis of adult mouse busulfan-treated azoospermia model. SSCs and sertoli cells were isolated from the testes of three to six-day old male mice.To determine the purity, Flow cytometry technique using PLZF antibody were evaluated. Isolated testicular cells were cultured in αMEM medium in the absence (control group) or presence (experimental group) of sertoli cells and melatonin extract for 2 weeks. We then transplanted SSCs by injection into the azoospermia mice model. Higher viability, proliferation, and Id4, Plzf, expression were observed in the presence of simultaneous sertoli cells and melatonin in vitro. Moreover, immunocytochemistry results showed higher Oct4 expression in this group. Eight weeks after transplantation, injected cells were localized at the base of seminiferous tubules in the recipient testes. The number of spermatogonia and the weight of testis were higher in the experimental group relative to control group. The results of our study suggest that this new protocol can increase the transplantation of these cells can be useful in the treatment of male infertility. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=colonization" title="colonization">colonization</a>, <a href="https://publications.waset.org/abstracts/search?q=melatonin" title=" melatonin"> melatonin</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatogonial%20stem%20cell" title=" spermatogonial stem cell"> spermatogonial stem cell</a>, <a href="https://publications.waset.org/abstracts/search?q=transplantation" title=" transplantation"> transplantation</a> </p> <a href="https://publications.waset.org/abstracts/84259/co-culture-of-neonate-mouse-spermatogonial-stem-cells-with-sertoli-cells-inductive-role-of-melatonin-following-transplantation-adult-azoospermia-mouse-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/84259.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">170</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3084</span> Comparison of Transparent Nickel Doped Cobalt Sulfide and Platinum Counter Electrodes Used in Quasi-Solid State Dye Sensitized Solar Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dimitra%20Sygkridou">Dimitra Sygkridou</a>, <a href="https://publications.waset.org/abstracts/search?q=Dimitrios%20Karageorgopoulos"> Dimitrios Karageorgopoulos</a>, <a href="https://publications.waset.org/abstracts/search?q=Elias%20Stathatos"> Elias Stathatos</a>, <a href="https://publications.waset.org/abstracts/search?q=Evangelos%20Vitoratos"> Evangelos Vitoratos</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Transparent nickel doped cobalt sulfide was fabricated on a SnO2:F electrode and tested as an efficient electrocatalyst and as an alternative to the expensive platinum counter electrode. In order to investigate how this electrode could affect the electrical characteristics of a dye-sensitized solar cell, we manufactured cells with the same TiO2 photoanode sensitized with dye (N719) and employing the same quasi-solid electrolyte, altering only the counter electrode used. The cells were electrically and electrochemically characterized and it was observed that the ones with the Ni doped CoS2 outperformed the efficiency of the cells with the Pt counter electrode (3.76% and 3.44% respectively). Particularly, the higher efficiency of the cells with the Ni doped CoS2 counter electrode (CE) is mainly because of the enhanced photocurrent density which is attributed to the enhanced electrocatalytic ability of the CE and the low charge transfer resistance at the CE/electrolyte interface. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nickel%20doped%20cobalt%20sulfide" title="nickel doped cobalt sulfide">nickel doped cobalt sulfide</a>, <a href="https://publications.waset.org/abstracts/search?q=counter%20electrodes" title=" counter electrodes"> counter electrodes</a>, <a href="https://publications.waset.org/abstracts/search?q=dye-sensitized%20solar%20cells" title=" dye-sensitized solar cells"> dye-sensitized solar cells</a>, <a href="https://publications.waset.org/abstracts/search?q=quasi-solid%20state%20electrolyte" title=" quasi-solid state electrolyte"> quasi-solid state electrolyte</a>, <a href="https://publications.waset.org/abstracts/search?q=hybrid%20organic-inorganic%20materials" title=" hybrid organic-inorganic materials"> hybrid organic-inorganic materials</a> </p> <a href="https://publications.waset.org/abstracts/29157/comparison-of-transparent-nickel-doped-cobalt-sulfide-and-platinum-counter-electrodes-used-in-quasi-solid-state-dye-sensitized-solar-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29157.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">760</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3083</span> Performance and Lifetime of Tandem Organic Solar Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Guillaume%20Schuchardt">Guillaume Schuchardt</a>, <a href="https://publications.waset.org/abstracts/search?q=Solenn%20Berson"> Solenn Berson</a>, <a href="https://publications.waset.org/abstracts/search?q=Gerard%20Perrier"> Gerard Perrier</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Multi-junction solar cell configurations, where two sub-cells with complementary absorption are stacked and connected in series, offer an exciting approach to tackle the single junction limitations of organic solar cells and improve their power conversion efficiency. However, the augmentation of the number of layers has, as a consequence, to increase the risk of reducing the lifetime of the cell due to the ageing phenomena present at the interfaces. In this work, we study the intrinsic degradation mechanisms, under continuous illumination AM1.5G, inert atmosphere and room temperature, in single and tandem organic solar cells using Impedance Spectroscopy, IV Curves, External Quantum Efficiency, Steady-State Photocarrier Grating, Scanning Kelvin Probe and UV-Visible light. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=single%20and%20tandem%20organic%20solar%20cells" title="single and tandem organic solar cells">single and tandem organic solar cells</a>, <a href="https://publications.waset.org/abstracts/search?q=intrinsic%20degradation%20mechanisms" title=" intrinsic degradation mechanisms"> intrinsic degradation mechanisms</a>, <a href="https://publications.waset.org/abstracts/search?q=characterization%3A%20SKP" title=" characterization: SKP"> characterization: SKP</a>, <a href="https://publications.waset.org/abstracts/search?q=EQE" title=" EQE"> EQE</a>, <a href="https://publications.waset.org/abstracts/search?q=SSPG" title=" SSPG"> SSPG</a>, <a href="https://publications.waset.org/abstracts/search?q=UV-Visible" title=" UV-Visible"> UV-Visible</a>, <a href="https://publications.waset.org/abstracts/search?q=Impedance%20Spectroscopy" title=" Impedance Spectroscopy"> Impedance Spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=optical%20simulation" title=" optical simulation"> optical simulation</a> </p> <a href="https://publications.waset.org/abstracts/46112/performance-and-lifetime-of-tandem-organic-solar-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46112.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">362</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3082</span> Removal of Samarium in Environmental Water Samples by Modified Yeast Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Homayon%20Ahmad%20Panahi">Homayon Ahmad Panahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Seyed%20Mehdi%20Seyed%20Nejad"> Seyed Mehdi Seyed Nejad</a>, <a href="https://publications.waset.org/abstracts/search?q=Elham%20Moniri"> Elham Moniri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A novel bio-adsorbent is fabricated by attaching a cibacron blue to yeast cells. The modified bio-sorbent has been characterized by some techniques like Fourier transform infrared spectroscopy (FT-IR) and elemental analysis (CHN) and applied for the preconcentration and determination of samarium from aqueous water samples. The best pH value for adsorption of the brilliant crecyle blue by yeast cells- cibacron blue was 7. The sorption capacity of modified biosorbent was 18.5 mg. g⁻¹. A recovery of 95.3% was obtained for Sm(III) when eluted with 0.5 M nitric acid. The method was applied for Sm(III) preconcentration and determination in river water sample. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=samarium" title="samarium">samarium</a>, <a href="https://publications.waset.org/abstracts/search?q=solid%20phase%20extraction" title=" solid phase extraction"> solid phase extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=yeast%20cells" title=" yeast cells"> yeast cells</a>, <a href="https://publications.waset.org/abstracts/search?q=water%20sample" title=" water sample"> water sample</a>, <a href="https://publications.waset.org/abstracts/search?q=removal" title=" removal"> removal</a> </p> <a href="https://publications.waset.org/abstracts/76122/removal-of-samarium-in-environmental-water-samples-by-modified-yeast-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/76122.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">255</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3081</span> Viscoelastic Separation and Concentration of Candida Using a Low Aspect Ratio Microchannel</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Seonggil%20Kim">Seonggil Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Jeonghun%20Nam"> Jeonghun Nam</a>, <a href="https://publications.waset.org/abstracts/search?q=Chae%20Seung%20Lim"> Chae Seung Lim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rapid diagnosis of fungal infections is critical for rapid antifungal therapy. However, it is difficult to detect extremely low concentration fungi in blood sample. To address the limitation, separation and concentration of fungi in blood sample are required to enhance the sensitivity of PCR analysis. In this study, we demonstrated a sheathless separation and concentration of fungi, candida cells using a viscoelastic fluid. To validate the performance of the device, microparticle mixture (2 and 13 μm) was used, and those particles were successfully separated based on the size difference at high flow rate of 100 μl/min. For the final application, successful separation of the Candida cells from the white blood cells (WBCs) was achieved. Based on the viscoelastic lateral migration toward the equilibrium position, Candida cells were separated and concentrated by center focusing, while WBCs were removed by patterning into two streams between the channel center and the sidewalls. By flow cytometric analysis, the separation efficiency and the purity were evaluated as ~99% and ~ 97%, respectively. From the results, the device can be the powerful tool for detecting extremely rare disease-related cells. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=candida%20cells" title="candida cells">candida cells</a>, <a href="https://publications.waset.org/abstracts/search?q=concentration" title=" concentration"> concentration</a>, <a href="https://publications.waset.org/abstracts/search?q=separation" title=" separation"> separation</a>, <a href="https://publications.waset.org/abstracts/search?q=viscoelastic%20fluid" title=" viscoelastic fluid"> viscoelastic fluid</a> </p> <a href="https://publications.waset.org/abstracts/90895/viscoelastic-separation-and-concentration-of-candida-using-a-low-aspect-ratio-microchannel" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/90895.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">198</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3080</span> Based on MR Spectroscopy, Metabolite Ratio Analysis of MRI Images for Metastatic Lesion</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hossain%20A">Hossain A</a>, <a href="https://publications.waset.org/abstracts/search?q=Hossain%20S."> Hossain S.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: In a small cohort, we sought to assess the magnetic resonance spectroscopy's (MRS) ability to predict the presence of metastatic lesions. Method: A Popular Diagnostic Centre Limited enrolled patients with neuroepithelial tumors. The 1H CSI MRS of the brain allows us to detect changes in the concentration of specific metabolites caused by metastatic lesions. Among these metabolites are N-acetyl-aspartate (NNA), creatine (Cr), and choline (Cho). For Cho, NAA, Cr, and Cr₂, the metabolic ratio was calculated using the division method. Results: The NAA values were 0.63 and 5.65 for tumor cells, 1.86 and 5.66 for normal cells, and 1.86 and 5.66 for normal cells 2. NAA values for normal cells 1 were 1.84, 10.6, and 1.86 for normal cells 2, respectively. Cho levels were as low as 0.8 and 10.53 in the tumor cell, compared to 1.12 and 2.7 in the normal cell 1 and 1.24 and 6.36 in the normal cell 2. Cho/Cr₂ barely distinguished itself from the other ratios in terms of significance. For tumor cells, the ratios of Cho/NAA, Cho/Cr₂, NAA/Cho, and NAA/Cr₂ were significant. Normal cell 1 had significant Cho/NAA, Cho/Cr, NAA/Cho, and NAA/Cr ratios. Conclusion: The clinical result can be improved by using 1H-MRSI to guide the size of resection for metastatic lesions. Even though it is non-invasive and doesn't present any difficulties during the procedure, MRS has been shown to predict the detection of metastatic lesions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=metabolite%20ratio" title="metabolite ratio">metabolite ratio</a>, <a href="https://publications.waset.org/abstracts/search?q=MRI%20images" title=" MRI images"> MRI images</a>, <a href="https://publications.waset.org/abstracts/search?q=metastatic%20lesion" title=" metastatic lesion"> metastatic lesion</a>, <a href="https://publications.waset.org/abstracts/search?q=MR%20spectroscopy" title=" MR spectroscopy"> MR spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=N-acetyl-aspartate" title=" N-acetyl-aspartate"> N-acetyl-aspartate</a> </p> <a href="https://publications.waset.org/abstracts/154048/based-on-mr-spectroscopy-metabolite-ratio-analysis-of-mri-images-for-metastatic-lesion" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/154048.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">96</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3079</span> Evaluation of Gene Expression after in Vitro Differentiation of Human Bone Marrow-Derived Stem Cells to Insulin-Producing Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mahmoud%20M.%20Zakaria">Mahmoud M. Zakaria</a>, <a href="https://publications.waset.org/abstracts/search?q=Omnia%20F.%20Elmoursi"> Omnia F. Elmoursi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmoud%20M.%20Gabr"> Mahmoud M. Gabr</a>, <a href="https://publications.waset.org/abstracts/search?q=Camelia%20A.%20AbdelMalak"> Camelia A. AbdelMalak</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20A.%20Ghoneim"> Mohamed A. Ghoneim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Many protocols were publicized for differentiation of human mesenchymal stem cells (MSCS) into insulin-producing cells (IPCs) in order to excrete insulin hormone ingoing to treat diabetes disease. Our aim is to evaluate relative gene expression for each independent protocol. Human bone marrow cells were derived from three volunteers that suffer diabetes disease. After expansion of mesenchymal stem cells, differentiation of these cells was done by three different protocols (the one-step protocol was used conophylline protein, the two steps protocol was depending on trichostatin-A, and the three-step protocol was started by beta-mercaptoethanol). Evaluation of gene expression was carried out by real-time PCR: Pancreatic endocrine genes, transcription factors, glucose transporter, precursor markers, pancreatic enzymes, proteolytic cleavage, extracellular matrix and cell surface protein. Quantitation of insulin secretion was detected by immunofluorescence technique in 24-well plate. Most of the genes studied were up-regulated in the in vitro differentiated cells, and also insulin production was observed in the three independent protocols. There were some slight increases in expression of endocrine mRNA of two-step protocol and its insulin production. So, the two-step protocol was showed a more efficient in expressing of pancreatic endocrine genes and its insulin production than the other two protocols. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mesenchymal%20stem%20cells" title="mesenchymal stem cells">mesenchymal stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=insulin%20producing%20cells" title=" insulin producing cells"> insulin producing cells</a>, <a href="https://publications.waset.org/abstracts/search?q=conophylline%20protein" title=" conophylline protein"> conophylline protein</a>, <a href="https://publications.waset.org/abstracts/search?q=trichostatin-A" title=" trichostatin-A"> trichostatin-A</a>, <a href="https://publications.waset.org/abstracts/search?q=beta-mercaptoethanol" title=" beta-mercaptoethanol"> beta-mercaptoethanol</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20expression" title=" gene expression"> gene expression</a>, <a href="https://publications.waset.org/abstracts/search?q=immunofluorescence%20technique" title=" immunofluorescence technique"> immunofluorescence technique</a> </p> <a href="https://publications.waset.org/abstracts/85954/evaluation-of-gene-expression-after-in-vitro-differentiation-of-human-bone-marrow-derived-stem-cells-to-insulin-producing-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/85954.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">215</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3078</span> Azadirachta indica Derived Protein Encapsulated Novel Guar Gum Nanocapsules against Colon Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Suman%20Chaudhary">Suman Chaudhary</a>, <a href="https://publications.waset.org/abstracts/search?q=Rupinder%20K.%20Kanwar"> Rupinder K. Kanwar</a>, <a href="https://publications.waset.org/abstracts/search?q=Jagat%20R.%20Kanwar"> Jagat R. Kanwar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Azadirachta indica, also known as Neem belonging to the mahogany family is actively gaining interest in the era of modern day medicine due to its extensive applications in homeopathic medicine such as Ayurveda and Unani. More than 140 phytochemicals have been extracted from neem leaves, seed, bark and flowers for agro-medicinal applications. Among the various components, neem leaf protein (NLP) is currently the most investigated active ingredient, due to its immunomodulatory activities against tumor growth. However, these therapeutic ingredients of neem are susceptible to degradation and cannot withstand the drastic pH changes under physiological environment, and therefore, there is an urgent need of an alternative strategy such as a nano-delivery system to exploit its medicinal benefits. This study hypothesizes that guar gum (GG) derived biodegradable nano-carrier based encapsulation of NLP will improve its stability, specificity and sensitivity, thus facilitating targeted anti-cancer therapeutics. GG is a galactomannan derived from the endosperm of the guar beans seeds. Synthesis of guar nanocapsules (NCs) was performed using nanoprecipitation technique where the GG was encapsulated with NLP. Preliminary experiments conducted to characterize the NCs confirmed spherical morphology with a narrow size distribution of 30-40 nm. Differential scanning colorimetric analysis (DSC) validated the stability of these NCs even at a temperature range of 50-60°C which was well within the physiological and storage conditions. Thermogravimetric (TGA) analysis indicated high decomposition temperature of these NCs ranging upto 350°C. Additionally, Fourier Transform Infrared spectroscopy (FTIR) and the SDS-PAGE data acquired confirmed the successful encapsulation of NLP in the NCs. The anti-cancerous therapeutic property of this NC was tested on colon cancer cells (caco-2) as they are one of the most prevalent form of cancer. These NCs (both NLP loaded and void) were also tested on human intestinal epithelial cells (FHs 74) cells to evaluate their effect on normal cells. Cytotoxicity evaluation of the NCs in the cell lines confirmed that the IC50 for NLP in FHs 74 cells was ~2 fold higher than in caco-2 cells, indicating that this nanoformulation system possessed biocompatible anti-cancerous properties Immunoconfocal microscopy analysis confirmed the time dependent internalization of the NCs within 6h. Recent findings performed using Annexin V and PI staining indicated a significant increase (p ≤ 0.001) in the early and late apoptotic cell population when treated with the NCs signifying the role of NLP in inducing apoptosis in caco-2 cells. This was further validated using Western blot, Polymerase chain reaction (PCR) and Fluorescence activated cell sorter (FACS) aided protein expressional analysis which presented a downregulation of survivin, an anti-apoptotic cell marker and upregulation of Bax/Bcl-2 ratio (pro-apoptotic indicator). Further, both the NLP NC and unencapsulated NLP treatment destabilized the mitochondrial membrane potential subsequently facilitating the release of the pro-apoptotic caspase cascade initiator, cytochrome-c. Future studies will be focused towards granting specificity to these NCs towards cancer cells, along with a comprehensive analysis of the anti-cancer potential of this naturally occurring compound in different cancer and in vivo animal models, will validate the clinical application of this unprecedented protein therapeutic. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anti-tumor" title="anti-tumor">anti-tumor</a>, <a href="https://publications.waset.org/abstracts/search?q=guar%20gum" title=" guar gum"> guar gum</a>, <a href="https://publications.waset.org/abstracts/search?q=nanocapsules" title=" nanocapsules"> nanocapsules</a>, <a href="https://publications.waset.org/abstracts/search?q=neem%20leaf%20protein" title=" neem leaf protein"> neem leaf protein</a> </p> <a href="https://publications.waset.org/abstracts/72624/azadirachta-indica-derived-protein-encapsulated-novel-guar-gum-nanocapsules-against-colon-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72624.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">177</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3077</span> A Novel Application of CORDYCEPIN (Cordycepssinensis Extract): Maintaining Stem Cell Pluripotency and Improving iPS Generation Efficiency</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shih-Ping%20Liu">Shih-Ping Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheng-Hsuan%20Chang"> Cheng-Hsuan Chang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu-Chuen%20Huang"> Yu-Chuen Huang</a>, <a href="https://publications.waset.org/abstracts/search?q=Shih-Yin%20Chen"> Shih-Yin Chen</a>, <a href="https://publications.waset.org/abstracts/search?q=Woei-Cherng%20Shyu"> Woei-Cherng Shyu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Embryonic stem cells (ES) and induced pluripotnet stem cells (iPS) are both pluripotent stem cells. For mouse stem cells culture technology, leukemia inhibitory factor (LIF) was used to maintain the pluripotency of stem cells in vitro. However, LIF is an expensive reagent. The goal of this study was to find out a pure compound extracted from Chinese herbal medicine that could maintain stem cells pluripotency to replace LIF and improve the iPS generation efficiency. From 20 candidates traditional Chinese medicine we found that Cordycepsmilitaris triggered the up-regulation of stem cells activating genes (Oct4 and Sox2) expression levels in MEF cells. Cordycepin, a major active component of Cordycepsmilitaris, also could up-regulate Oct4 and Sox2 gene expression. Furthermore, we used ES and iPS cells and treated them with different concentrations of Cordycepin (replaced LIF in the culture medium) to test whether it was useful to maintain the pluripotency. The results showed higher expression levels of several stem cells markers in 10 μM Cordycepin-treated ES and iPS cells compared to controls that did not contain LIF, including alkaline phosphatase, SSEA1, and Nanog. Embryonic body formation and differentiation confirmed that 10 μM Cordycepin-containing medium was capable to maintain stem cells pluripotency after four times passages. For mechanism analysis, microarray analysis indicated extracellular matrix and Jak/Stat signaling pathway as the top two deregulated pathways. In ECM pathway, we determined that the integrin αVβ5 expression levels and phosphorylated Src levels increased after Cordycepin treatment. In addition, the phosphorylated Jak2 and phosphorylated Sat3 protein levels were increased after Cordycepin treatment and suppressed with the Jak2 inhibitor, AG490. The expression of cytokines associated with Jak2/Stat3 signaling pathway were also up-regulated by Q-PCR and ELISA assay. Lastly, we used Oct4-GFP MEF cells to test iPS generation efficiency following Cordycepin treatment. We observed that 10 Μm Cordycepin significantly increased the iPS generation efficiency in day 21. In conclusion, we demonstrated Cordycepin could maintain the pluripotency of stem cells through both of ECM and Jak2/Stat3 signaling pathway and improved iPS generation efficiency. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cordycepin" title="cordycepin">cordycepin</a>, <a href="https://publications.waset.org/abstracts/search?q=iPS%20cells" title=" iPS cells"> iPS cells</a>, <a href="https://publications.waset.org/abstracts/search?q=Jak2%2FStat3%20signaling%20pathway" title=" Jak2/Stat3 signaling pathway"> Jak2/Stat3 signaling pathway</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20biology" title=" molecular biology"> molecular biology</a> </p> <a href="https://publications.waset.org/abstracts/6862/a-novel-application-of-cordycepin-cordycepssinensis-extract-maintaining-stem-cell-pluripotency-and-improving-ips-generation-efficiency" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/6862.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">438</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3076</span> The Comparison between bFGF and Small Molecules in Derivation of Chicken Primordial Germ Cells and Embryonic Germ Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Farzaneh">Maryam Farzaneh</a>, <a href="https://publications.waset.org/abstracts/search?q=Seyyedeh%20Nafiseh%20Hassani"> Seyyedeh Nafiseh Hassani</a>, <a href="https://publications.waset.org/abstracts/search?q=Hossein%20Baharvand"> Hossein Baharvand </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: Chicken gonadal tissue has a two population such primordial germ cells (PGCs) and stromal cells (somatic cells). PGCs and embryonic germ cells (EGCs) that is a pluripotent type of PGCs in long-term culture are suitable sources for the production of chicken pluripotent stem cell lines, transgenic birds, vaccine and recombinant protein production. In general, the effect of growth factors such bFGF and mouse LIF on derivation of PGCs in vitro are important and in this study we could see the unique effect of small molecules such PD032 and SB43 as a chemical, in comparison to growth factors. Materials and Methods: After incubation of fertilized chicken egg up to 6 days and isolation of primary gonadal tissues and culture of mixed cells like PGCs and stromal cells. PGCs proliferate in the present of fetal calf serum (FCS) and small molecules and in another group bFGF, that these factors are important for PGCs culture and derivation. Somatic cells produce a multilayer feeder under the PGCs in primary culture and PGCs make a small cluster under these cells. Results: In present of small molecules and high volume of FCS (15%), the present of EGCs as a pluripotent stem cells were clear four weeks, that they had a positive immune-staining and periodic acid-Schiff staining (PAS), but in present of growth factors like bFGF without any chemicals, the present of PGCs were clear but after 7 until 10 days, there were disappear. Conclusion: Until now we have seen many researches about derivation and maintenance of chicken PGCs, in the hope of understanding the mechanisms that occur during germline development and production of a therapeutic product by transgenic birds. There are still many unknowns in this area and this project will try to have efficient conditions for identification of suitable culture medium for long-term culture of PGCs in vitro without serum and feeder cells. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chicken%20gonadal%20primordial%20germ%20cells" title="chicken gonadal primordial germ cells">chicken gonadal primordial germ cells</a>, <a href="https://publications.waset.org/abstracts/search?q=pluripotent%20stem%20cells" title=" pluripotent stem cells"> pluripotent stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=growth%20factors" title=" growth factors"> growth factors</a>, <a href="https://publications.waset.org/abstracts/search?q=small%20molecules" title=" small molecules"> small molecules</a>, <a href="https://publications.waset.org/abstracts/search?q=transgenic%20birds" title=" transgenic birds"> transgenic birds</a> </p> <a href="https://publications.waset.org/abstracts/34508/the-comparison-between-bfgf-and-small-molecules-in-derivation-of-chicken-primordial-germ-cells-and-embryonic-germ-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/34508.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">434</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3075</span> Activation of Apoptosis in the Midgut Epithelium of Spodoptera exigua Hübner (Lepidoptera: Noctuidae) Exposed to Various Cadmium Concentration</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Magdalena%20Maria%20Rost-Roszkowska">Magdalena Maria Rost-Roszkowska</a>, <a href="https://publications.waset.org/abstracts/search?q=Alina%20Chachulska-%C5%BByme%C5%82ka"> Alina Chachulska-Żymełka</a>, <a href="https://publications.waset.org/abstracts/search?q=Monika%20Tarnawska"> Monika Tarnawska</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Augustyniak"> Maria Augustyniak</a>, <a href="https://publications.waset.org/abstracts/search?q=Alina%20Kafel"> Alina Kafel</a>, <a href="https://publications.waset.org/abstracts/search?q=Agnieszka%20Babczy%C5%84ska"> Agnieszka Babczyńska</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The digestive system of insects is composed of three distinct regions: fore-, mid- and hingut. The middle region (the midgut) is treated as one of the barriers which protects the organism against any stressors which originate from external environment, e.g. toxic metals. Such factors can activate the cell death in epithelial cells to preserve the entire tissue/organs against the degeneration. Different mechanisms involved in homeostasis maintenance have been described, but the studies of animals under field conditions do not give the opportunity to conclude about potential ability of subsequent generation to inherit the tolerance mechanisms. It is possible only by a multigenerational strain of an animal led under laboratory conditions, exposed to a selected toxic factor, present also in polluted ecosystems. The main purpose of the project was to check if changes, which appear in the midgut epithelium after Cd treatment, can be fixed during the following generations of insects with the special emphasis on apoptosis. As the animal for these studies we chose 5th larval stage of the beet armyworm Spodoptera exigua Hübner (Lepidoptera: Noctuidae), which is one of pest of many vegetable crops. Animals were divided into some experimental groups: K, Cd, KCd, Cd1, Cd2, Cd3. A control group (K) fed a standard diet, and was conducted for XX generations, a cadmium group (Cd), fed on standard diet supplemented with cadmium (44 mg Cd per kg of dry weight of food) for XXX generations. A reference Cd group (KCd) has been initiated: control insects were fed with Cd supplemented diet (44 mg Cd per kg of dry weight of food). Experimental groups Cd1, Cd2, Cd3 developed from the control one: 5 mg Cd per kg of dry weight of food, 10 mg Cd per kg of dry weight of food, 20 mg Cd per kg of dry weight of food. We were interested in the activation of apoptosis during following generations in all experimental groups. Therefore, during the 1st year of the experiment, the measurements were done for 6 generations in all experimental group. The intensity and the course of apoptosis have been examined using transmission electron microscope (TEM), confocal microscope and flow cytometry. During apoptosis the cell started to shrink, extracellular spaces appeared between digestive and neighboring cells, the nucleus achieved a lobular shape. Eventually, the apoptotic cells was discharged into the midgut lumen. A quantitative analysis revealed that the number of apoptotic cells depends significantly on the generation, tissue and cadmium concentration in the insect rearing medium. In the following 6 generations, we observed that the percentage of apoptotic cells in the midguts from cadmium-exposed groups decreased gradually according to the following order of strains: Cd1, Cd2, Cd3 and KCd. At the same time, it was still higher than the percentage of apoptotic cells in the same tissues of the insects from the control and multigenerational cadmium strain. The results of our studies suggest that changes caused by cadmium treatment were preserved during 6-generational development of lepidopteran larvae. The study has been financed by the National Science Centre Poland, grant no 2016/21/B/NZ8/00831. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cadmium" title="cadmium">cadmium</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20death" title=" cell death"> cell death</a>, <a href="https://publications.waset.org/abstracts/search?q=digestive%20system" title=" digestive system"> digestive system</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrastructure" title=" ultrastructure"> ultrastructure</a> </p> <a href="https://publications.waset.org/abstracts/90370/activation-of-apoptosis-in-the-midgut-epithelium-of-spodoptera-exigua-hubner-lepidoptera-noctuidae-exposed-to-various-cadmium-concentration" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/90370.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">214</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3074</span> Chemical Modification of Biosorbent for Prconcentation of Cadmium in Water Sample</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Homayon%20Ahmad%20Panahi">Homayon Ahmad Panahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Niusha%20Mohseni%20Darabi"> Niusha Mohseni Darabi</a>, <a href="https://publications.waset.org/abstracts/search?q=Elham%20Moniri"> Elham Moniri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A new biosorbent is prepared by coupling a cibacron blue to yeast cells. The modified yeast cells with cibacron blue has been characterized by Fourier transform infrared spectroscopy (FT-IR) and elemental analysis and applied for the preconcentration and solid phase extraction of trace cadmium ion from water samples. The optimum pH value for sorption of the cadmium ions by yeast cells- cibacron blue was 5.5. The sorption capacity of modified biosorbent was 45 mg. g−1. A recovery of 98.2% was obtained for Cd(II) when eluted with 0.5 M nitric acid. The method was applied for Cd(II) preconcentration and determination in sea water sample. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solid%20phase%20extraction" title="solid phase extraction">solid phase extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=yeast%20cells" title=" yeast cells"> yeast cells</a>, <a href="https://publications.waset.org/abstracts/search?q=Nickl" title=" Nickl"> Nickl</a>, <a href="https://publications.waset.org/abstracts/search?q=isotherm%20study" title=" isotherm study"> isotherm study</a> </p> <a href="https://publications.waset.org/abstracts/52871/chemical-modification-of-biosorbent-for-prconcentation-of-cadmium-in-water-sample" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/52871.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">264</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3073</span> iPSC-derived MSC Mediated Immunosuppression during Mouse Airway Transplantation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Afzal%20Khan">Mohammad Afzal Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Fatimah%20Alanazi"> Fatimah Alanazi</a>, <a href="https://publications.waset.org/abstracts/search?q=Hala%20Abdalrahman%20Ahmed"> Hala Abdalrahman Ahmed</a>, <a href="https://publications.waset.org/abstracts/search?q=Talal%20Shamma"> Talal Shamma</a>, <a href="https://publications.waset.org/abstracts/search?q=Kilian%20Kelly"> Kilian Kelly</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20A.%20Hammad"> Mohammed A. Hammad</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdullah%20O.%20Alawad"> Abdullah O. Alawad</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdullah%20Mohammed%20Assiri"> Abdullah Mohammed Assiri</a>, <a href="https://publications.waset.org/abstracts/search?q=Dieter%20Clemens%20Broering"> Dieter Clemens Broering</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lung transplantation is a life-saving surgical replacement of diseased lungs in patients with end-stage respiratory malfunctions. Despite the remarkable short-term recovery, long-term lung survival continues to face several significant challenges, including chronic rejection and severe toxic side-effects due to global immunosuppression. Stem cell-based immunotherapy has been recognized as a crucial immunoregulatory regimen in various preclinical and clinical studies. Despite initial therapeutic outcomes, conventional stem cells face key limitations. The Cymerus™ manufacturing facilitates the production of a virtually limitless supply of consistent human induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells, which could play a key role in selective immunosuppression and graft repair during rejection. Here, we demonstrated the impact of iPSC-derived human MSCs on the development of immune-tolerance and long-term graft survival in mouse orthotopic airway allografts. BALB/c→C57BL/6 allografts were reconstituted with iPSC-derived MSCs (2 million/transplant/ at d0), and allografts were examined for regulatory T cells (Tregs), oxygenation, microvascular blood flow, airway epithelium and collagen deposition during rejection. We demonstrated that iPSC-derived MSC treatment leads to significant increase in tissue expression of hTSG-6 protein, followed by an upregulation of mouse Tregs and IL-5, IL-10, IL-15 cytokines, which augments graft microvascular blood flow and oxygenation, and thereby maintained a healthy airway epithelium and prevented the subepithelial deposition of collagen at d90 post-transplantation. Collectively, these data confirmed that iPSC-derived MSC-mediated immunosuppression has potential to establish immune-tolerance and rescue allograft from sustained hypoxic/ischemic phase and subsequently limits long-term airway epithelial injury and collagen progression, which therapeutically warrant a study of Cymerus iPSC-derived MSCs as a potential management option for immunosuppression in transplant recipients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=stem%20cell%20therapy" title="stem cell therapy">stem cell therapy</a>, <a href="https://publications.waset.org/abstracts/search?q=immunotolerance" title=" immunotolerance"> immunotolerance</a>, <a href="https://publications.waset.org/abstracts/search?q=regulatory%20T%20cells" title=" regulatory T cells"> regulatory T cells</a>, <a href="https://publications.waset.org/abstracts/search?q=hypoxia%20and%20ischemia" title=" hypoxia and ischemia"> hypoxia and ischemia</a>, <a href="https://publications.waset.org/abstracts/search?q=microvasculature" title=" microvasculature"> microvasculature</a> </p> <a href="https://publications.waset.org/abstracts/114237/ipsc-derived-msc-mediated-immunosuppression-during-mouse-airway-transplantation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/114237.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">158</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3072</span> The Role of Il-6-Mediated NS5ATP9 Expression in Autophagy of Liver Cancer Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hongping%20Lu">Hongping Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=Kelbinur%20%20Tursun"> Kelbinur Tursun</a>, <a href="https://publications.waset.org/abstracts/search?q=Yaru%20Li"> Yaru Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu%20Zhang"> Yu Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Shunai%20Liu"> Shunai Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Ming%20Han"> Ming Han</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: To investigate whether NS5ATP9 is involved in IL-6 mediated autophagy and the relationship between IL-6 and NS5ATP9 in liver cancer cells. Methods: 1. Detect the mRNA and protein levels of Beclin 1 after HepG2 cells were treated with or without recombinant human IL-6 protein. 2. Measure and compare of the changes of autophagy-related genes with their respective control, after IL-6 was silenced or neutralized with monoclonal antibody against human IL-6. 3. HepG2 cells were incubated with 50 ng/ml of IL-6 in the presence or absence of PDTC. The expression of NS5ATP9 was analyzed by Western blot after 48 h. 4. After NS5ATP9-silenced HepG2 cells had been treated with 50 ng/ml recombinant IL-6 protein, we detected the Beclin 1 and LC3B (LC3Ⅱ/Ⅰ) expression. 5. HepG2 cells were transfected with pNS5ATP9, si-NS5ATP9, and their respective control. Total RNA was isolated from cells and analyzed for IL-6. 6. Silence or neutralization of IL-6 in HepG2 cells which has been transfected with NS5ATP9. Beclin 1 and LC3 protein levels were analyzed by Western blot. Result: 1. After HepG2 were treated with recombinant human IL-6 protein, the expression of endogenous Beclin 1 was up-regulated at mRNA and protein level, and the conversion of endogenous LC3-I to LC3-II was also increased. These results indicated that IL-6 could induce autophagy. 2. When HepG2 cells were treated with IL-6 siRNA or monoclonal antibody against human IL-6, the expression of autophagy-related genes were decreased. 3. Exogenous human IL-6 recombinant protein up-regulated NS5ATP9 via NF-κB activation. 4. The expression of Beclin 1 and LC3B was down-regulated after IL-6 treated NS5ATP9-silenced HepG2 cells. 5. NS5ATP9 could reverse regulates IL-6 expression in HepG2 cells. 6. Silence or neutralization of IL-6 attenuates NS5ATP9-induced autophagy slightly. Conclusion: Our results implied that in HCC patients, maybe the higher level of IL-6 in the serum promoted the expression of NS5ATP9 and induced autophagy in cancer cells. And the over-expression of NS5ATP9 which induced by IL-6, in turn, increased IL-6 expression, further, promotes the IL-6/NS5ATP9-mediated autophagy and affects the progression of tumor. Therefore, NS5ATP9 silence might be a potential target for HCC therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=autophagy" title="autophagy">autophagy</a>, <a href="https://publications.waset.org/abstracts/search?q=Hepatocellular%20carcinoma" title=" Hepatocellular carcinoma"> Hepatocellular carcinoma</a>, <a href="https://publications.waset.org/abstracts/search?q=IL-6" title=" IL-6"> IL-6</a>, <a href="https://publications.waset.org/abstracts/search?q=microenvironment" title=" microenvironment"> microenvironment</a>, <a href="https://publications.waset.org/abstracts/search?q=NS5ATP9" title=" NS5ATP9"> NS5ATP9</a> </p> <a href="https://publications.waset.org/abstracts/58075/the-role-of-il-6-mediated-ns5atp9-expression-in-autophagy-of-liver-cancer-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58075.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">250</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3071</span> Aerobic Exercise Increases Circulating Hematopoietic Stem Cells and Endothelial Progenitor Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Khaled%20A.%20shady">Khaled A. shady</a>, <a href="https://publications.waset.org/abstracts/search?q=Fagr%20B.%20Bazeed"> Fagr B. Bazeed</a>, <a href="https://publications.waset.org/abstracts/search?q=Nashwa%20K.%20Abousamra"> Nashwa K. Abousamra</a>, <a href="https://publications.waset.org/abstracts/search?q=Ihab%20H.%20Elberawe"> Ihab H. Elberawe</a>, <a href="https://publications.waset.org/abstracts/search?q=Ashraf%20E.%20shaalan"> Ashraf E. shaalan</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20A.%20Sobh"> Mohamed A. Sobh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Physical activity activates a variety of adult stem cells which might be released into the circulation or might be activated in their organ-resident state. A variety of stimuli such as metabolic, mechanical, and hormonal stimuli might by responsible for the mobilization. This study was done to know the changes in hematopoietic stem cells and endothelial progenitor in athletes in the 24 hours following 30 min of aerobic exercise. Methods: Ten healthy male's athlete's (age 20.7± 0.61 y) performed moderate running with 30 min at 80% of velocity of The IAT. Blood samples taken pre-, and immediately, 30 min, 2h, 6h and 24h post-exercise were analyzed for hematopoietic stem cells (HSCs ), endothelial progenitor cells (EPCs(, vascular endothelial growth factor (VEGF), nitric oxide (NO), lactic acid (LA), and white blood cells . HSCs and EPCs were quantified by flow cytometry. Results: After 30min of aerobic exercise significant increases in HSCs, EPC, VEGF, NO, LA and WBCs (p ˂ 0.05). This increase will be at different rates according to the timing of taking blood sample and was in the maximum rate of increase after 30 min of aerobic exercise. HSCs, EPC, NO and WBCs were in the maximum rate of increase 2h post exercise. In addition, VEGF was in the maximum rate of increase immediately post exercise and LA concentration not affected after exercise. Conclusion: These data suggest that HSCs and EPCs increased after aerobic exercise due to increase of VEGF which play an important role in mobilization of stem cells and promotes NO increase which contributes to increase EPCs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=physical%20activity" title="physical activity">physical activity</a>, <a href="https://publications.waset.org/abstracts/search?q=hematopoietic%20stem%20cells" title=" hematopoietic stem cells"> hematopoietic stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=mobilization" title=" mobilization"> mobilization</a>, <a href="https://publications.waset.org/abstracts/search?q=athletes" title=" athletes"> athletes</a> </p> <a href="https://publications.waset.org/abstracts/158031/aerobic-exercise-increases-circulating-hematopoietic-stem-cells-and-endothelial-progenitor-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/158031.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">117</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3070</span> Graphene Materials for Efficient Hybrid Solar Cells: A Spectroscopic Investigation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20Khenfouch">Mohammed Khenfouch</a>, <a href="https://publications.waset.org/abstracts/search?q=Fokotsa%20V.%20Molefe"> Fokotsa V. Molefe</a>, <a href="https://publications.waset.org/abstracts/search?q=Bakang%20M.%20Mothudi"> Bakang M. Mothudi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Nowadays, graphene and its composites are universally known as promising materials. They show their potential in a large field of applications including photovoltaics. This study reports on the role of nanohybrids and nanosystems known as strong light harvesters in the efficiency of graphene hybrid solar cells. Our system included Graphene/ZnO/Porphyrin/P3HT layers. Moreover, the physical properties including surface/interface, optical and vibrational properties were also studied. Our investigations confirmed the interaction between the different components as well as the sensitivity of their photonics to the synthesis conditions. Remarkable energy and charge transfer were detected and deeply investigated. Hence, the optimization of the conditions will lead to the fabrication of higher conversion efficiency in graphene solar cells. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=graphene" title="graphene">graphene</a>, <a href="https://publications.waset.org/abstracts/search?q=optoelectronics" title=" optoelectronics"> optoelectronics</a>, <a href="https://publications.waset.org/abstracts/search?q=nanohybrids" title=" nanohybrids"> nanohybrids</a>, <a href="https://publications.waset.org/abstracts/search?q=solar%20cells" title=" solar cells"> solar cells</a> </p> <a href="https://publications.waset.org/abstracts/80659/graphene-materials-for-efficient-hybrid-solar-cells-a-spectroscopic-investigation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80659.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">168</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3069</span> Induction of Apoptosis by Diosmin through Interleukins/STAT and Mitochondria Mediated Pathway in Hep-2 and KB Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Rajasekar">M. Rajasekar</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Suresh"> K. Suresh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diosmin is a flavonoid, most abundantly found in many citrus fruits. As a flavonoid, it possesses a multitude of biological activities including anti-hyperglycemic, anti-lipid peroxidative, anti-inflammatory, antioxidant, and anti-mutagenic properties. At this point, we established the anti-proliferative and apoptosis-inducing activities of diosmin in Hep-2 and KB cells. Diosmin has cytotoxic effects through inhibiting cellular proliferation of Hep-2 and KB cells, which leads to the induction of apoptosis, as apparent by an increase in the fraction of cells in the sub-G1phase of the cell cycle. Results exposed that inhibition of cell proliferation is associated with regulation of the Interleukins/STAT pathway. In addition, Diosmin treatment with Hep-2 and KB cells actively stimulated reactive oxygen species (ROS) and mitochondrial membrane depolarization. And also an imbalance in the Bax/Bcl-2 ratio triggered the caspase cascade and shifting the balance in favor of apoptosis. These observations conclude that Diosmin induce apoptosis via Interleukins /STAT-mediated pathway. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diosmin" title="diosmin">diosmin</a>, <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title=" apoptosis"> apoptosis</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=STAT%20pathway" title=" STAT pathway"> STAT pathway</a> </p> <a href="https://publications.waset.org/abstracts/37353/induction-of-apoptosis-by-diosmin-through-interleukinsstat-and-mitochondria-mediated-pathway-in-hep-2-and-kb-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37353.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">328</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3068</span> Following the Modulation of Transcriptional Activity of Genes by Chromatin Modifications during the Cell Cycle in Living Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sharon%20Yunger">Sharon Yunger</a>, <a href="https://publications.waset.org/abstracts/search?q=Liat%20Altman"> Liat Altman</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuval%20Garini"> Yuval Garini</a>, <a href="https://publications.waset.org/abstracts/search?q=Yaron%20Shav-Tal"> Yaron Shav-Tal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Understanding the dynamics of transcription in living cells has improved since the development of quantitative fluorescence-based imaging techniques. We established a method for following transcription from a single copy gene in living cells. A gene tagged with MS2 repeats, used for mRNA tagging, in its 3' UTR was integrated into a single genomic locus. The actively transcribing gene was detected and analyzed by fluorescence in situ hybridization (FISH) and live-cell imaging. Several cell clones were created that differed in the promoter regulating the gene. Thus, comparative analysis could be obtained without the risk of different position effects at each integration site. Cells in S/G2 phases could be detected exhibiting two adjacent transcription sites on sister chromatids. A sharp reduction in the transcription levels was observed as cells progressed along the cell cycle. We hypothesized that a change in chromatin structure acts as a general mechanism during the cell cycle leading to down-regulation in the activity of some genes. We addressed this question by treating the cells with chromatin decondensing agents. Quantifying and imaging the treated cells suggests that chromatin structure plays a role both in regulating transcriptional levels along the cell cycle, as well as in limiting an active gene from reaching its maximum transcription potential at any given time. These results contribute to understanding the role of chromatin as a regulator of gene expression. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cell%20cycle" title="cell cycle">cell cycle</a>, <a href="https://publications.waset.org/abstracts/search?q=living%20cells" title=" living cells"> living cells</a>, <a href="https://publications.waset.org/abstracts/search?q=nucleus" title=" nucleus"> nucleus</a>, <a href="https://publications.waset.org/abstracts/search?q=transcription" title=" transcription"> transcription</a> </p> <a href="https://publications.waset.org/abstracts/40812/following-the-modulation-of-transcriptional-activity-of-genes-by-chromatin-modifications-during-the-cell-cycle-in-living-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40812.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">311</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3067</span> Induction of G1 Arrest and Apoptosis in Human Cancer Cells by Panaxydol</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dong-Gyu%20Leem">Dong-Gyu Leem</a>, <a href="https://publications.waset.org/abstracts/search?q=Ji-Sun%20Shin"> Ji-Sun Shin</a>, <a href="https://publications.waset.org/abstracts/search?q=Sang%20Yoon%20Choi"> Sang Yoon Choi</a>, <a href="https://publications.waset.org/abstracts/search?q=Kyung-Tae%20Lee"> Kyung-Tae Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, we focused on the anti-proliferative effects of panaxydol, a C17 polyacetylenic compound derived from Panax ginseng roots, against various human cancer cells. We treated with panaxydol to various cancer cells and panaxydol treatment was found to significantly inhibit the proliferation of human lung cancer cells (A549) and human pancreatic cancer cells (AsPC-1 and MIA PaCa-2), of which AsPC-1 cells were most sensitive to its treatment. DNA flow cytometric analysis indicated that panaxydol blocked cell cycle progression at the G1 phase in A549 cells, which accompanied by a parallel reduction of protein expression of cyclin-dependent kinase (CDK) 2, CDK4, CDK6, cyclin D1 and cyclin E. CDK inhibitors (CDKIs), such as p21CIP1/WAF1 and p27KIP1, were gradually upregulated after panaxydol treatment at the protein levels. Furthermore, panaxydol induced the activation of p53 in A549 cells. In addition, panaxydol also induced apoptosis of AsPC-1 and MIA PaCa-2 cells, as shown by accumulation of subG1 and apoptotic cell populations. Panaxydol triggered the activation of caspase-3, -8, -9 and the cleavage of poly (ADP-ribose) polymerase (PARP). Reduction of mitochondrial transmembrane potential by panaxydol was determined by staining with dihexyloxacarbocyanine iodide. Furthermore, panaxydol suppressed the levels of anti-apoptotic proteins, XIAP and Bcl-2, and increased the levels of proapoptotic proteins, Bax and Bad. In addition, panaxydol inhibited the activation of Akt and extracellular signal-regulated kinase (ERK) and activated the p38 mitogen-activated protein kinase kinase (MAPK). Our results suggest that panaxydol is an anti-tumor compound that causes p53-mediated cell cycle arrest and apoptosis via mitochondrial apoptotic pathway in various cancer cells. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title="apoptosis">apoptosis</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer" title=" cancer"> cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=G1%20arrest" title=" G1 arrest"> G1 arrest</a>, <a href="https://publications.waset.org/abstracts/search?q=panaxydol" title=" panaxydol"> panaxydol</a> </p> <a href="https://publications.waset.org/abstracts/50141/induction-of-g1-arrest-and-apoptosis-in-human-cancer-cells-by-panaxydol" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/50141.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">322</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3066</span> Effect of Povidone Iodine in Treatment of Epidemic Keratoconjunctivitis: Clinical Trail Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Hossain%20Validad">Mohammad Hossain Validad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background and Aim: Epidemic keratoconjunctivitis is a type of conjunctivitis caused by adenoviruses that can spread rapidly through direct and indirect contact. The aim of this study was to evaluate the therapeutic effects of Povidone-Iodine 0.4% and 0.2% in improving the symptoms and signs of patients with epidemic keratoconjunctivitis. Materials and Methods: In this clinical trial study, 60 patients with a mean age of 27.8±8.4 years who were eligible for inclusion criteria were randomly divided into three groups. The first group received eye drops of Povidone-Iodine 0.4% and betamethasone 0.1%, the second group received PovidoneIodine 0.2% and betamethasone 0.1% and the third group received betamethasone 0.1%. Follow-ups were on the first, fourth, seventh and tenth days after starting treatment. Parameters examined at each examination were hyperaemia, mucopurulent discharge, eyelid edema, hemorrhage, and subepithelial infiltration. Results: The results showed that mucopurulent discharge on the fourth day of the examination (P = 0.005) and the seventh day of the examination (P = 0.001) were significantly different in the three treatment groups. Sub-epithelial infiltration on the tenth day after treatment did not show a significant difference in the 3 groups (P = 0.287). Conclusion: Based on the results of this study, Povidone-Iodine is more effective in relieving some of signs of EKC, such as reduced mucopurulent discharge than steroids alone. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=EKC" title="EKC">EKC</a>, <a href="https://publications.waset.org/abstracts/search?q=topical%20bethadine" title=" topical bethadine"> topical bethadine</a>, <a href="https://publications.waset.org/abstracts/search?q=adenovirus" title=" adenovirus"> adenovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=sub%20epithelial%20opacity" title=" sub epithelial opacity"> sub epithelial opacity</a> </p> <a href="https://publications.waset.org/abstracts/165494/effect-of-povidone-iodine-in-treatment-of-epidemic-keratoconjunctivitis-clinical-trail-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165494.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">76</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3065</span> Lipid-polymer Nanocarrier Platform Enables X-Ray Induced Photodynamic Therapy against Human Colorectal Cancer Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rui%20Sang">Rui Sang</a>, <a href="https://publications.waset.org/abstracts/search?q=Fei%20Deng"> Fei Deng</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexander%20Engel"> Alexander Engel</a>, <a href="https://publications.waset.org/abstracts/search?q=Ewa%20M.%20Goldys"> Ewa M. Goldys</a>, <a href="https://publications.waset.org/abstracts/search?q=Wei%20Deng"> Wei Deng</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, we brought together X-ray induced photodynamic therapy (X-PDT) and chemo-drug (5-FU) for the treatment on colorectal cancer cells. This was achieved by developing a lipid-polymer hybrid nanoparticle delivery system (FA-LPNPs-VP-5-FU). It was prepared by incorporating a photosensitizer (verteporfin), chemotherapy drug (5-FU), and a targeting moiety (folic acid) into one platform. The average size of these nanoparticles was around 100 nm with low polydispersity. When exposed to clinical doses of 4 Gy X-ray radiation, FA-LPNPs-VP-5-FU generated sufficient amounts of reactive oxygen species, triggering the apoptosis and necrosis pathway of cancer cells. Our combined X-PDT and chemo-drug strategy was effective in inhibiting cancer cells’ growth and proliferation. Cell cycle analyses revealed that our treatment induced G2/M and S phase arrest in HCT116 cells. Our results indicate that this combined treatment provides better antitumour effect in colorectal cancer cells than each of these modalities alone. This may offer a novel approach for effective colorectal cancer treatment with reduced off-target effect and drug toxicity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pdt" title="pdt">pdt</a>, <a href="https://publications.waset.org/abstracts/search?q=targeted%20lipid-polymer%20nanoparticles" title=" targeted lipid-polymer nanoparticles"> targeted lipid-polymer nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=verteporfin" title=" verteporfin"> verteporfin</a>, <a href="https://publications.waset.org/abstracts/search?q=colorectal%20cancer" title=" colorectal cancer"> colorectal cancer</a> </p> <a href="https://publications.waset.org/abstracts/164493/lipid-polymer-nanocarrier-platform-enables-x-ray-induced-photodynamic-therapy-against-human-colorectal-cancer-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164493.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">76</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3064</span> Hyaluronic Acid - Alginate Hydrogel for the Transdifferentiation of Testis Cells into Erythrocyte and Hepatocyte-like Cells; A Practice Within an Effective Agent Choice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Leila%20Rashki%20Ghaleno">Leila Rashki Ghaleno</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamad%20Amin%20Hajari"> Mohamad Amin Hajari</a>, <a href="https://publications.waset.org/abstracts/search?q=Leila%20Montazeri"> Leila Montazeri</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdolhossein%20Shahverdi"> Abdolhossein Shahverdi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mojtaba%20Rezazadeh%20Valojerdi"> Mojtaba Rezazadeh Valojerdi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Spermatogonia stem cells (SSCs) exhibit pluripotency, enabling them to undergo differentiation into many cell lineages, including neurons, glia, endothelial cells, and hepatocytes when cultured in vitro. Although the specific mechanisms are not yet fully understood, it has been observed that biopolymer agents, such as hyaluronic acid (HA) and alginate (Alg), have the potential to induce transdifferentiation of SSCs. The current work aimed to examine the process of in vitro spermatogenesis and the conversion of mouse testicular cells into hepatocytes and erythrocyte-like cells utilizing the HA-Alg hydrogel. Method: After being extracted from the testes of a 5-day postpartum mouse (5 DPP), the testicular cells were separated into two enzymatic stages and then put into a composite hydrogel containing 0.5% HA and 1% alginate. On days 14 and 28 of culture, the colonies' growth, the cells' viability, and their histology were assessed. Result: Despite observing significant cell proliferation on day 14 and the development of circular-shaped organoids on day 28, it was noted that the organoids generated in the HA-Alg medium tended to maintain their circular morphology on day 28. Notably, the testicular cells underwent transdifferentiation into cell types resembling erythrocytes and hepatocytes. The hepatocyte-like cells exhibited the presence of glycogen and lipid deposits, indicating their hepatocyte-like characteristics. Interestingly, immunostaining analysis revealed the secretion of albumin and the presence of VEGFR on day 14. However, on day 28, albumin expression was not detected, while the expression of Sox9 (a marker for hepatocytes), Vegf, CD34, and C-kit (markers for erythrocytes) showed increased levels in the gene expression evaluation. Conclusion: The present findings indicated that HA-Alg could be a potent and effective agent for the transdifferentiation of testis cells into erythrocyte and hepatocyte-like cells, as recent studies have confirmed the transformation of SSCs into hepatocyte cells during in vitro culture. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=3D%20culture" title="3D culture">3D culture</a>, <a href="https://publications.waset.org/abstracts/search?q=mouse%20testicular%20cell" title=" mouse testicular cell"> mouse testicular cell</a>, <a href="https://publications.waset.org/abstracts/search?q=hyaluronic%20acid" title=" hyaluronic acid"> hyaluronic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=liver%20organoids" title=" liver organoids"> liver organoids</a> </p> <a href="https://publications.waset.org/abstracts/171962/hyaluronic-acid-alginate-hydrogel-for-the-transdifferentiation-of-testis-cells-into-erythrocyte-and-hepatocyte-like-cells-a-practice-within-an-effective-agent-choice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171962.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">71</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3063</span> Simulation of Remove the Fouling on the in vivo By Using MHD </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Farhad%20Aalizadeh">Farhad Aalizadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Ali%20Moosavi"> Ali Moosavi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> When a blood vessel is injured, the cells of your blood bond together to form a blood clot. The blood clot helps you stop bleeding. Blood clots are made of a combination of blood cells, platelets(small sticky cells that speed up the clot-making process), and fibrin (protein that forms a thread-like mesh to trap cells). Doctors call this kind of blood clot a “thrombus.”We study the effects of different parameters on the deposition of Nanoparticles on the surface of a bump in the blood vessels by the magnetic field. The Maxwell and the flow equations are solved for this purpose. It is assumed that the blood is non-Newtonian and the number of particles has been considered enough to rely on the results statistically. Using MHD and its property it is possible to control the flow velocity, remove the fouling on the walls and return the system to its original form. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=MHD" title="MHD">MHD</a>, <a href="https://publications.waset.org/abstracts/search?q=fouling" title=" fouling"> fouling</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vivo" title=" in-vivo"> in-vivo</a>, <a href="https://publications.waset.org/abstracts/search?q=blood%20clots" title=" blood clots"> blood clots</a>, <a href="https://publications.waset.org/abstracts/search?q=simulation" title=" simulation"> simulation</a> </p> <a href="https://publications.waset.org/abstracts/14099/simulation-of-remove-the-fouling-on-the-in-vivo-by-using-mhd" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/14099.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">469</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3062</span> Sitagliptin-AntiCD4 Mab Conjugated T Cell Targeting Therapy for the Effective Treatment of Type I Diabetes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=T.%20Mahesh">T. Mahesh</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20K.%20Samanta"> M. K. Samanta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Antibody dug conjugate (ADC’s) concept is a less explored and more trustable for the treatment of Type 1 diabetes (T1D). T1D is thought to arise from selective immunologically mediated destruction of the insulin- producing β-cells in the pancreatic islets of Langerhans with consequent insulin deficiency. It is evident that type 1 diabetes can be conquered, by 1) to stop immune destruction of βcells, 2) to replace or regenerate β-cells, and 3) to preserve β-cell function and mass. Many studies found that the regulatory T cells (Tregs) are crucial for the maintenance of immunological tolerance. Immune tolerance is liable for the activation of the Th1 response. The important role of Th1 response in pathology of T1D entails the depletion of CD4+ T cells, which initiated the use of anti-CD4 monoclonal antibodies (mAbs) against CD4+ T cells to interfere with induction of T1D.Insulin is regulated by Glucagon-Like Peptide-1 hormone (GLP-1) which also stimulates β-cells proliferation as the half-life of GLP-1 harmone is less due to rapid degradation by DPP-IV enzyme an alternative DPP-IV-inhibitors can increase the half-life of GLP-1 through which it conquers the replacement and reserve β-cells mass. Thus in the present study Anti-CD4 mAb was conjugated with Sitagliptin which is a DPP-IV inhibitor Drug loaded in Nanoparticles through Sulfo-MBS cross-linkers. The above study can be an effective approach for treatment to overcome the Passive subcutaneous insulin therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibody%20drug%20conjugates" title="antibody drug conjugates">antibody drug conjugates</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-CD4%20Mab" title=" anti-CD4 Mab"> anti-CD4 Mab</a>, <a href="https://publications.waset.org/abstracts/search?q=DPP%20IV%20inhibitors" title=" DPP IV inhibitors"> DPP IV inhibitors</a>, <a href="https://publications.waset.org/abstracts/search?q=GLP-1" title=" GLP-1"> GLP-1</a> </p> <a href="https://publications.waset.org/abstracts/21545/sitagliptin-anticd4-mab-conjugated-t-cell-targeting-therapy-for-the-effective-treatment-of-type-i-diabetes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21545.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">389</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3061</span> Bcl-2: A Molecule to Detect Oral Cancer and Precancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vandana%20Singh">Vandana Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Subash%20Singh"> Subash Singh </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Oral squamous cell carcinoma is the most common malignant tumor of the oral cavity. Normally the death of cell and the growth are active processes and depend not only on external factors but also on the expression of genes like Bcl-2, which activate and inhibit apoptosis. The term Bcl-2 is an acronym for B-cell lymphoma/ leukemia -2 genes. Objectives: An attempt was made to evaluate Bcl-2 oncoprotein expression in patients with oral precancer and cancer and to assess possible correlation between Bcl-2 oncoprotein expression and clinicopathological features of oral precancer and cancer. Material and Methods: This is a selective prospective clinical and immunohistochemical study. Clinicopathological examination is correlated with immunohistochemical findings. The immunolocalization of Bcl-2 protein is performed using the labeled streptavidin biotin (LSAB) method. To visualize the reaction, 3, 3-diaminobenzidine (DAB) is used. Results: Bcl-2 expression was positive in 11 [36.66 %, low Bcl-2 expression 3 (10.00 %), moderate Bcl-2 expression 7 (23.33 %), and high Bcl-2 expression 1 (3.33 %)] oral cancer cases and in 14 [87.50 %, low expression 8 (50 %), moderate expression 6 (37.50 %)] precancer cases. Conclusion: On the basis of the results of our study we conclude that positive Bcl-2 expression may be an indicator of poor prognosis in oral cancer and precancer. Relevance: It has been reported that there is deregulation of Bcl-2 expression during progression from oral epithelial dysplasia to squamous cell carcinoma. It can be used for revealing progression of epithelial dysplasia to malignancy and as a prognostic marker in oral precancer and cancer. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=BcL-2" title="BcL-2">BcL-2</a>, <a href="https://publications.waset.org/abstracts/search?q=immunohistochemistry" title=" immunohistochemistry"> immunohistochemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=oral%20cancer" title=" oral cancer"> oral cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=oral%20precancer" title=" oral precancer"> oral precancer</a> </p> <a href="https://publications.waset.org/abstracts/79418/bcl-2-a-molecule-to-detect-oral-cancer-and-precancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/79418.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">269</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3060</span> Discover a New Technique for Cancer Recognition by Analysis and Determination of Fractal Dimension Images in Matlab Software</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saeedeh%20Shahbazkhany">Saeedeh Shahbazkhany</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cancer is a terrible disease that, if not diagnosed early, therapy can be difficult while it is easily medicable if it is diagnosed in early stages. So it is very important for cancer diagnosis that medical procedures are performed. In this paper we introduce a new method. In this method, we only need pictures of healthy cells and cancer cells. In fact, where we suspect cancer, we take a picture of cells or tissue in that area, and then take some pictures of the surrounding tissues. Then, fractal dimension of images are calculated and compared. Cancer can be easily detected by comparing the fractal dimension of images. In this method, we use Matlab software. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Matlab%20software" title="Matlab software">Matlab software</a>, <a href="https://publications.waset.org/abstracts/search?q=fractal%20dimension" title=" fractal dimension"> fractal dimension</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer" title=" cancer"> cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=surrounding%20tissues" title=" surrounding tissues"> surrounding tissues</a>, <a href="https://publications.waset.org/abstracts/search?q=cells%20or%20tissue" title=" cells or tissue"> cells or tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=new%20method" title=" new method"> new method</a> </p> <a href="https://publications.waset.org/abstracts/8641/discover-a-new-technique-for-cancer-recognition-by-analysis-and-determination-of-fractal-dimension-images-in-matlab-software" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/8641.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">354</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3059</span> From Dog to Dog: Potential Probiotic and Immunomodulatory Strains Isolated from Canine Milk</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Paula%20Buldres">Paula Buldres</a>, <a href="https://publications.waset.org/abstracts/search?q=Jorge%20Toledo"> Jorge Toledo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objectives: This study aimed to characterize potential probiotic strains isolated from canine breast milk for use in dogs with enteropathies. Methodology: Six canine breast milk strains, one canine colostrum strain, and one control porcine breast milk strain were characterized. According to its functional properties of resistance to acids, different concentrations of bile salts, and pancreatin, its presumptive properties of safety and inhibitory effect on pathogens, non-cytotoxic characteristics, and adhesion to the intestine. The immunomodulatory effect of formulations with better probiotic characterization in vitro and in vivo was also analyzed. Results: Two strains characterized as potential probiotics were obtained, which corresponded to the canine strains (TUCO-16 and TUCO-17), presenting resistance to acidic pH, bile salts, and pancreatin, as well as an inhibitory effect on pathogenic Escherichia coli, Salmonella sp., and Clostridium perfringens. Strains TUCO-16 and TUCO-17 induced a significant increase in the expression of TNF-α and IL-8 in canine macrophages, respectively. Expression analyses of pattern recognition receptors in DH82 cells suggest that TUCO-16 and TUCO-17 might increase the TLR2 expression marker, and porcine strain (TUCO-4) increases the NOD2 expression marker. Based on the count obtained and the encapsulation yield, the best formulations correspond to FOS-Inulin for the TUCO-17 and TUCO-4 strains; Maltodextrin-Inulin for TUCO-16. All the strains are non-cytotoxic. The strain that showed the highest adhesion to intestinal epithelial cells was TUCO-17 with the FOS-Inulin formulation. On the other hand, the probiotics decreased the expression of pro-inflammatory markers in vivo, both in the intestine and in the spleen of mice. Conclusion: The combination of these three strains under study (TUCO-16, TUCO-17, and TUCO-4) would cover the probiotic properties in formulation and immunomodulation of all the markers under study. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=probiotics" title="probiotics">probiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=gastrointestinal%20infec" title=" gastrointestinal infec"> gastrointestinal infec</a>, <a href="https://publications.waset.org/abstracts/search?q=dog" title=" dog"> dog</a>, <a href="https://publications.waset.org/abstracts/search?q=probiotic%20formulation" title=" probiotic formulation"> probiotic formulation</a>, <a href="https://publications.waset.org/abstracts/search?q=immunomodulatory%20probiotics" title=" immunomodulatory probiotics"> immunomodulatory probiotics</a> </p> <a href="https://publications.waset.org/abstracts/163977/from-dog-to-dog-potential-probiotic-and-immunomodulatory-strains-isolated-from-canine-milk" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/163977.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">68</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3058</span> Genotoxic and Cytotoxic Effects of Methidathion Pesticide</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Y.%20Alfaifi">Mohammad Y. Alfaifi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Methidathion (MTD) (Trade name Supracide®) is a non-systemic organophosphorus insecticide used intensively worldwide including Saudi Arabia. However, there is a lack in published studies about it's genotoxicity. In this study we evaluated MTD toxicity in rat bone marrow cells (in vivo) and in lymphocytes (in vitro) using different doses based on LD50. MNNCE (Micronucleated normocromatic erythrocytes) and MNPCE (Micronucleated polychromatic erythrocytes), NDI (Nuclear division index) and NDCI (nuclear division cytotoxicity index), necrotic and apoptotic cells were recorded in rat's bone marrow samples. CA, MI (number of cells undergoing mitosis) necrotic, and apoptotic cells recorded in lymphocytes. Results showed that there was a slight increase in the frequency of micronucleated bone marrow cells. However, no structural chromosomal aberrations were detected in vivo or in vitro. On the other hand, the results showed significant increase in necrotic and apoptotic cells following MTD administration in a dose-dependent manner comparing to positive and negative control groups. In light of these results, MTD can be considered highly cytotoxic and moderate genotoxic, and precaution should be taken when using MTD. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=methidathion" title="methidathion">methidathion</a>, <a href="https://publications.waset.org/abstracts/search?q=micronucleus" title=" micronucleus"> micronucleus</a>, <a href="https://publications.waset.org/abstracts/search?q=NDI" title=" NDI"> NDI</a>, <a href="https://publications.waset.org/abstracts/search?q=NDCI" title=" NDCI"> NDCI</a>, <a href="https://publications.waset.org/abstracts/search?q=toxicity" title=" toxicity"> toxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=chromosomal%20aberrations" title=" chromosomal aberrations"> chromosomal aberrations</a> </p> <a href="https://publications.waset.org/abstracts/2877/genotoxic-and-cytotoxic-effects-of-methidathion-pesticide" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2877.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">412</span> </span> </div> </div> <ul class="pagination"> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=7" rel="prev">&lsaquo;</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=1">1</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=2">2</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=6">6</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=7">7</a></li> <li class="page-item active"><span class="page-link">8</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=9">9</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=10">10</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=11">11</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=109">109</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=110">110</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=corneal%20epithelial%20cells&page=9" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); });*/ jQuery.get({ url: "https://publications.waset.org/xhr/user-menu", cache: false }).then(function(response){ jQuery('#mainNavMenu').append(response); }); }); </script> </body> </html>